TOP1 cDNA ORF clone, Homo sapiens(Human)

The following TOP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TOP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu27246 NM_003286.4
Latest version!
Homo sapiens DNA topoisomerase I (TOP1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $265.30
$379.00
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OHu27246 NM_003286.3 Homo sapiens DNA topoisomerase I (TOP1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $265.30
$379.00
OHu27246 NM_003286.2 Homo sapiens topoisomerase (DNA) I (TOP1), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $265.30
$379.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu27246
    Clone ID Related Accession (Same CDS sequence) NM_003286.4 , NM_003286.3 , NM_003286.2
    Accession Version NM_003286.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-09
    Organism Homo sapiens(human)
    Product DNA topoisomerase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA690829.1, AK292943.1 and BC000943.2. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_003286.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U07806.1, SRR1660803.104236.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000361337.3/ ENSP00000354522.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGTGGGG ACCACCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
    TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCACAAAGA ACACAAGAAG
    GAGAAGGACC GGGAAAAGTC CAAGCATAGC AACAGTGAAC ATAAAGATTC TGAAAAGAAA
    CACAAAGAGA AGGAGAAGAC CAAACACAAA GATGGAAGCT CAGAAAAGCA TAAAGACAAA
    CATAAAGACA GAGACAAGGA AAAACGAAAA GAGGAAAAGG TTCGAGCCTC TGGGGATGCA
    AAAATAAAGA AGGAGAAGGA AAATGGCTTC TCTAGTCCAC CACAAATTAA AGATGAACCT
    GAAGATGATG GCTATTTTGT TCCTCCTAAA GAGGATATAA AGCCATTAAA GAGACCTCGA
    GATGAGGATG ATGCTGATTA TAAACCTAAG AAAATTAAAA CAGAAGATAC CAAGAAGGAG
    AAGAAAAGAA AACTAGAAGA AGAAGAGGAT GGTAAATTGA AAAAACCCAA GAATAAAGAT
    AAAGATAAAA AAGTTCCTGA GCCAGATAAC AAGAAAAAGA AGCCGAAGAA AGAAGAGGAA
    CAGAAGTGGA AATGGTGGGA AGAAGAGCGC TATCCTGAAG GCATCAAGTG GAAATTCCTA
    GAACATAAAG GTCCAGTATT TGCCCCACCA TATGAGCCTC TTCCAGAGAA TGTCAAGTTT
    TATTATGATG GTAAAGTCAT GAAGCTGAGC CCCAAAGCAG AGGAAGTAGC TACGTTCTTT
    GCAAAAATGC TCGACCATGA ATATACTACC AAGGAAATAT TTAGGAAAAA TTTCTTTAAA
    GACTGGAGAA AGGAAATGAC TAATGAAGAG AAGAATATTA TCACCAACCT AAGCAAATGT
    GATTTTACCC AGATGAGCCA GTATTTCAAA GCCCAGACGG AAGCTCGGAA ACAGATGAGC
    AAGGAAGAGA AACTGAAAAT CAAAGAGGAG AATGAAAAAT TACTGAAAGA ATATGGATTC
    TGTATTATGG ATAACCACAA AGAGAGGATT GCTAACTTCA AGATAGAGCC TCCTGGACTT
    TTCCGTGGCC GCGGCAACCA CCCCAAGATG GGCATGCTGA AGAGACGAAT CATGCCCGAG
    GATATAATCA TCAACTGTAG CAAAGATGCC AAGGTTCCTT CTCCTCCTCC AGGACATAAG
    TGGAAAGAAG TCCGGCATGA TAACAAGGTT ACTTGGCTGG TTTCCTGGAC AGAGAACATC
    CAAGGTTCCA TTAAATACAT CATGCTTAAC CCTAGTTCAC GAATCAAGGG TGAGAAGGAC
    TGGCAGAAAT ACGAGACTGC TCGGCGGCTG AAAAAATGTG TGGACAAGAT CCGGAACCAG
    TATCGAGAAG ACTGGAAGTC CAAAGAGATG AAAGTCCGGC AGAGAGCTGT AGCCCTGTAC
    TTCATCGACA AGCTTGCTCT GAGAGCAGGC AATGAAAAGG AGGAAGGAGA AACAGCGGAC
    ACTGTGGGCT GCTGCTCACT TCGTGTGGAG CACATCAATC TACACCCAGA GTTGGATGGT
    CAGGAATATG TGGTAGAGTT TGACTTCCTC GGGAAGGACT CCATCAGATA CTATAACAAG
    GTCCCTGTTG AGAAACGAGT TTTTAAGAAC CTACAACTAT TTATGGAGAA CAAGCAGCCC
    GAGGATGATC TTTTTGATAG ACTCAATACT GGTATTCTGA ATAAGCATCT TCAGGATCTC
    ATGGAGGGCT TGACAGCCAA GGTATTCCGT ACATACAATG CCTCCATCAC GCTACAGCAG
    CAGCTAAAAG AACTGACAGC CCCGGATGAG AACATCCCAG CGAAGATCCT TTCTTATAAC
    CGTGCCAATC GAGCTGTTGC AATTCTTTGT AACCATCAGA GGGCACCACC AAAAACTTTT
    GAGAAGTCTA TGATGAACTT GCAAACTAAG ATTGATGCCA AGAAGGAACA GCTAGCAGAT
    GCCCGGAGAG ACCTGAAAAG TGCTAAGGCT GATGCCAAGG TCATGAAGGA TGCAAAGACG
    AAGAAGGTAG TAGAGTCAAA GAAGAAGGCT GTTCAGAGAC TGGAGGAACA GTTGATGAAG
    CTGGAAGTTC AAGCCACAGA CCGAGAGGAA AATAAACAGA TTGCCCTGGG AACCTCCAAA
    CTCAATTATC TGGACCCTAG GATCACAGTG GCTTGGTGCA AGAAGTGGGG TGTCCCAATT
    GAGAAGATTT ACAACAAAAC CCAGCGGGAG AAGTTTGCCT GGGCCATTGA CATGGCTGAT
    GAAGACTATG AGTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003277.1
    CDS247..2544
    Translation

    Target ORF information:

    RefSeq Version NM_003286.4
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase I (TOP1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003286.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGTGGGG ACCACCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
    TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCACAAAGA ACACAAGAAG
    GAGAAGGACC GGGAAAAGTC CAAGCATAGC AACAGTGAAC ATAAAGATTC TGAAAAGAAA
    CACAAAGAGA AGGAGAAGAC CAAACACAAA GATGGAAGCT CAGAAAAGCA TAAAGACAAA
    CATAAAGACA GAGACAAGGA AAAACGAAAA GAGGAAAAGG TTCGAGCCTC TGGGGATGCA
    AAAATAAAGA AGGAGAAGGA AAATGGCTTC TCTAGTCCAC CACAAATTAA AGATGAACCT
    GAAGATGATG GCTATTTTGT TCCTCCTAAA GAGGATATAA AGCCATTAAA GAGACCTCGA
    GATGAGGATG ATGCTGATTA TAAACCTAAG AAAATTAAAA CAGAAGATAC CAAGAAGGAG
    AAGAAAAGAA AACTAGAAGA AGAAGAGGAT GGTAAATTGA AAAAACCCAA GAATAAAGAT
    AAAGATAAAA AAGTTCCTGA GCCAGATAAC AAGAAAAAGA AGCCGAAGAA AGAAGAGGAA
    CAGAAGTGGA AATGGTGGGA AGAAGAGCGC TATCCTGAAG GCATCAAGTG GAAATTCCTA
    GAACATAAAG GTCCAGTATT TGCCCCACCA TATGAGCCTC TTCCAGAGAA TGTCAAGTTT
    TATTATGATG GTAAAGTCAT GAAGCTGAGC CCCAAAGCAG AGGAAGTAGC TACGTTCTTT
    GCAAAAATGC TCGACCATGA ATATACTACC AAGGAAATAT TTAGGAAAAA TTTCTTTAAA
    GACTGGAGAA AGGAAATGAC TAATGAAGAG AAGAATATTA TCACCAACCT AAGCAAATGT
    GATTTTACCC AGATGAGCCA GTATTTCAAA GCCCAGACGG AAGCTCGGAA ACAGATGAGC
    AAGGAAGAGA AACTGAAAAT CAAAGAGGAG AATGAAAAAT TACTGAAAGA ATATGGATTC
    TGTATTATGG ATAACCACAA AGAGAGGATT GCTAACTTCA AGATAGAGCC TCCTGGACTT
    TTCCGTGGCC GCGGCAACCA CCCCAAGATG GGCATGCTGA AGAGACGAAT CATGCCCGAG
    GATATAATCA TCAACTGTAG CAAAGATGCC AAGGTTCCTT CTCCTCCTCC AGGACATAAG
    TGGAAAGAAG TCCGGCATGA TAACAAGGTT ACTTGGCTGG TTTCCTGGAC AGAGAACATC
    CAAGGTTCCA TTAAATACAT CATGCTTAAC CCTAGTTCAC GAATCAAGGG TGAGAAGGAC
    TGGCAGAAAT ACGAGACTGC TCGGCGGCTG AAAAAATGTG TGGACAAGAT CCGGAACCAG
    TATCGAGAAG ACTGGAAGTC CAAAGAGATG AAAGTCCGGC AGAGAGCTGT AGCCCTGTAC
    TTCATCGACA AGCTTGCTCT GAGAGCAGGC AATGAAAAGG AGGAAGGAGA AACAGCGGAC
    ACTGTGGGCT GCTGCTCACT TCGTGTGGAG CACATCAATC TACACCCAGA GTTGGATGGT
    CAGGAATATG TGGTAGAGTT TGACTTCCTC GGGAAGGACT CCATCAGATA CTATAACAAG
    GTCCCTGTTG AGAAACGAGT TTTTAAGAAC CTACAACTAT TTATGGAGAA CAAGCAGCCC
    GAGGATGATC TTTTTGATAG ACTCAATACT GGTATTCTGA ATAAGCATCT TCAGGATCTC
    ATGGAGGGCT TGACAGCCAA GGTATTCCGT ACATACAATG CCTCCATCAC GCTACAGCAG
    CAGCTAAAAG AACTGACAGC CCCGGATGAG AACATCCCAG CGAAGATCCT TTCTTATAAC
    CGTGCCAATC GAGCTGTTGC AATTCTTTGT AACCATCAGA GGGCACCACC AAAAACTTTT
    GAGAAGTCTA TGATGAACTT GCAAACTAAG ATTGATGCCA AGAAGGAACA GCTAGCAGAT
    GCCCGGAGAG ACCTGAAAAG TGCTAAGGCT GATGCCAAGG TCATGAAGGA TGCAAAGACG
    AAGAAGGTAG TAGAGTCAAA GAAGAAGGCT GTTCAGAGAC TGGAGGAACA GTTGATGAAG
    CTGGAAGTTC AAGCCACAGA CCGAGAGGAA AATAAACAGA TTGCCCTGGG AACCTCCAAA
    CTCAATTATC TGGACCCTAG GATCACAGTG GCTTGGTGCA AGAAGTGGGG TGTCCCAATT
    GAGAAGATTT ACAACAAAAC CCAGCGGGAG AAGTTTGCCT GGGCCATTGA CATGGCTGAT
    GAAGACTATG AGTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27246
    Clone ID Related Accession (Same CDS sequence) NM_003286.4 , NM_003286.3 , NM_003286.2
    Accession Version NM_003286.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-10
    Organism Homo sapiens(human)
    Product DNA topoisomerase 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA690829.1, AK292943.1 and BC000943.2. This sequence is a reference standard in the RefSeqGene project. On Sep 26, 2016 this sequence version replaced NM_003286.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U07806.1, SRR1803611.24870.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGTGGGG ACCACCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
    TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCACAAAGA ACACAAGAAG
    GAGAAGGACC GGGAAAAGTC CAAGCATAGC AACAGTGAAC ATAAAGATTC TGAAAAGAAA
    CACAAAGAGA AGGAGAAGAC CAAACACAAA GATGGAAGCT CAGAAAAGCA TAAAGACAAA
    CATAAAGACA GAGACAAGGA AAAACGAAAA GAGGAAAAGG TTCGAGCCTC TGGGGATGCA
    AAAATAAAGA AGGAGAAGGA AAATGGCTTC TCTAGTCCAC CACAAATTAA AGATGAACCT
    GAAGATGATG GCTATTTTGT TCCTCCTAAA GAGGATATAA AGCCATTAAA GAGACCTCGA
    GATGAGGATG ATGCTGATTA TAAACCTAAG AAAATTAAAA CAGAAGATAC CAAGAAGGAG
    AAGAAAAGAA AACTAGAAGA AGAAGAGGAT GGTAAATTGA AAAAACCCAA GAATAAAGAT
    AAAGATAAAA AAGTTCCTGA GCCAGATAAC AAGAAAAAGA AGCCGAAGAA AGAAGAGGAA
    CAGAAGTGGA AATGGTGGGA AGAAGAGCGC TATCCTGAAG GCATCAAGTG GAAATTCCTA
    GAACATAAAG GTCCAGTATT TGCCCCACCA TATGAGCCTC TTCCAGAGAA TGTCAAGTTT
    TATTATGATG GTAAAGTCAT GAAGCTGAGC CCCAAAGCAG AGGAAGTAGC TACGTTCTTT
    GCAAAAATGC TCGACCATGA ATATACTACC AAGGAAATAT TTAGGAAAAA TTTCTTTAAA
    GACTGGAGAA AGGAAATGAC TAATGAAGAG AAGAATATTA TCACCAACCT AAGCAAATGT
    GATTTTACCC AGATGAGCCA GTATTTCAAA GCCCAGACGG AAGCTCGGAA ACAGATGAGC
    AAGGAAGAGA AACTGAAAAT CAAAGAGGAG AATGAAAAAT TACTGAAAGA ATATGGATTC
    TGTATTATGG ATAACCACAA AGAGAGGATT GCTAACTTCA AGATAGAGCC TCCTGGACTT
    TTCCGTGGCC GCGGCAACCA CCCCAAGATG GGCATGCTGA AGAGACGAAT CATGCCCGAG
    GATATAATCA TCAACTGTAG CAAAGATGCC AAGGTTCCTT CTCCTCCTCC AGGACATAAG
    TGGAAAGAAG TCCGGCATGA TAACAAGGTT ACTTGGCTGG TTTCCTGGAC AGAGAACATC
    CAAGGTTCCA TTAAATACAT CATGCTTAAC CCTAGTTCAC GAATCAAGGG TGAGAAGGAC
    TGGCAGAAAT ACGAGACTGC TCGGCGGCTG AAAAAATGTG TGGACAAGAT CCGGAACCAG
    TATCGAGAAG ACTGGAAGTC CAAAGAGATG AAAGTCCGGC AGAGAGCTGT AGCCCTGTAC
    TTCATCGACA AGCTTGCTCT GAGAGCAGGC AATGAAAAGG AGGAAGGAGA AACAGCGGAC
    ACTGTGGGCT GCTGCTCACT TCGTGTGGAG CACATCAATC TACACCCAGA GTTGGATGGT
    CAGGAATATG TGGTAGAGTT TGACTTCCTC GGGAAGGACT CCATCAGATA CTATAACAAG
    GTCCCTGTTG AGAAACGAGT TTTTAAGAAC CTACAACTAT TTATGGAGAA CAAGCAGCCC
    GAGGATGATC TTTTTGATAG ACTCAATACT GGTATTCTGA ATAAGCATCT TCAGGATCTC
    ATGGAGGGCT TGACAGCCAA GGTATTCCGT ACATACAATG CCTCCATCAC GCTACAGCAG
    CAGCTAAAAG AACTGACAGC CCCGGATGAG AACATCCCAG CGAAGATCCT TTCTTATAAC
    CGTGCCAATC GAGCTGTTGC AATTCTTTGT AACCATCAGA GGGCACCACC AAAAACTTTT
    GAGAAGTCTA TGATGAACTT GCAAACTAAG ATTGATGCCA AGAAGGAACA GCTAGCAGAT
    GCCCGGAGAG ACCTGAAAAG TGCTAAGGCT GATGCCAAGG TCATGAAGGA TGCAAAGACG
    AAGAAGGTAG TAGAGTCAAA GAAGAAGGCT GTTCAGAGAC TGGAGGAACA GTTGATGAAG
    CTGGAAGTTC AAGCCACAGA CCGAGAGGAA AATAAACAGA TTGCCCTGGG AACCTCCAAA
    CTCAATTATC TGGACCCTAG GATCACAGTG GCTTGGTGCA AGAAGTGGGG TGTCCCAATT
    GAGAAGATTT ACAACAAAAC CCAGCGGGAG AAGTTTGCCT GGGCCATTGA CATGGCTGAT
    GAAGACTATG AGTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003277.1
    CDS251..2548
    Translation

    Target ORF information:

    RefSeq Version NM_003286.3
    Organism Homo sapiens(human)
    Definition Homo sapiens DNA topoisomerase I (TOP1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003286.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGTGGGG ACCACCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
    TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCACAAAGA ACACAAGAAG
    GAGAAGGACC GGGAAAAGTC CAAGCATAGC AACAGTGAAC ATAAAGATTC TGAAAAGAAA
    CACAAAGAGA AGGAGAAGAC CAAACACAAA GATGGAAGCT CAGAAAAGCA TAAAGACAAA
    CATAAAGACA GAGACAAGGA AAAACGAAAA GAGGAAAAGG TTCGAGCCTC TGGGGATGCA
    AAAATAAAGA AGGAGAAGGA AAATGGCTTC TCTAGTCCAC CACAAATTAA AGATGAACCT
    GAAGATGATG GCTATTTTGT TCCTCCTAAA GAGGATATAA AGCCATTAAA GAGACCTCGA
    GATGAGGATG ATGCTGATTA TAAACCTAAG AAAATTAAAA CAGAAGATAC CAAGAAGGAG
    AAGAAAAGAA AACTAGAAGA AGAAGAGGAT GGTAAATTGA AAAAACCCAA GAATAAAGAT
    AAAGATAAAA AAGTTCCTGA GCCAGATAAC AAGAAAAAGA AGCCGAAGAA AGAAGAGGAA
    CAGAAGTGGA AATGGTGGGA AGAAGAGCGC TATCCTGAAG GCATCAAGTG GAAATTCCTA
    GAACATAAAG GTCCAGTATT TGCCCCACCA TATGAGCCTC TTCCAGAGAA TGTCAAGTTT
    TATTATGATG GTAAAGTCAT GAAGCTGAGC CCCAAAGCAG AGGAAGTAGC TACGTTCTTT
    GCAAAAATGC TCGACCATGA ATATACTACC AAGGAAATAT TTAGGAAAAA TTTCTTTAAA
    GACTGGAGAA AGGAAATGAC TAATGAAGAG AAGAATATTA TCACCAACCT AAGCAAATGT
    GATTTTACCC AGATGAGCCA GTATTTCAAA GCCCAGACGG AAGCTCGGAA ACAGATGAGC
    AAGGAAGAGA AACTGAAAAT CAAAGAGGAG AATGAAAAAT TACTGAAAGA ATATGGATTC
    TGTATTATGG ATAACCACAA AGAGAGGATT GCTAACTTCA AGATAGAGCC TCCTGGACTT
    TTCCGTGGCC GCGGCAACCA CCCCAAGATG GGCATGCTGA AGAGACGAAT CATGCCCGAG
    GATATAATCA TCAACTGTAG CAAAGATGCC AAGGTTCCTT CTCCTCCTCC AGGACATAAG
    TGGAAAGAAG TCCGGCATGA TAACAAGGTT ACTTGGCTGG TTTCCTGGAC AGAGAACATC
    CAAGGTTCCA TTAAATACAT CATGCTTAAC CCTAGTTCAC GAATCAAGGG TGAGAAGGAC
    TGGCAGAAAT ACGAGACTGC TCGGCGGCTG AAAAAATGTG TGGACAAGAT CCGGAACCAG
    TATCGAGAAG ACTGGAAGTC CAAAGAGATG AAAGTCCGGC AGAGAGCTGT AGCCCTGTAC
    TTCATCGACA AGCTTGCTCT GAGAGCAGGC AATGAAAAGG AGGAAGGAGA AACAGCGGAC
    ACTGTGGGCT GCTGCTCACT TCGTGTGGAG CACATCAATC TACACCCAGA GTTGGATGGT
    CAGGAATATG TGGTAGAGTT TGACTTCCTC GGGAAGGACT CCATCAGATA CTATAACAAG
    GTCCCTGTTG AGAAACGAGT TTTTAAGAAC CTACAACTAT TTATGGAGAA CAAGCAGCCC
    GAGGATGATC TTTTTGATAG ACTCAATACT GGTATTCTGA ATAAGCATCT TCAGGATCTC
    ATGGAGGGCT TGACAGCCAA GGTATTCCGT ACATACAATG CCTCCATCAC GCTACAGCAG
    CAGCTAAAAG AACTGACAGC CCCGGATGAG AACATCCCAG CGAAGATCCT TTCTTATAAC
    CGTGCCAATC GAGCTGTTGC AATTCTTTGT AACCATCAGA GGGCACCACC AAAAACTTTT
    GAGAAGTCTA TGATGAACTT GCAAACTAAG ATTGATGCCA AGAAGGAACA GCTAGCAGAT
    GCCCGGAGAG ACCTGAAAAG TGCTAAGGCT GATGCCAAGG TCATGAAGGA TGCAAAGACG
    AAGAAGGTAG TAGAGTCAAA GAAGAAGGCT GTTCAGAGAC TGGAGGAACA GTTGATGAAG
    CTGGAAGTTC AAGCCACAGA CCGAGAGGAA AATAAACAGA TTGCCCTGGG AACCTCCAAA
    CTCAATTATC TGGACCCTAG GATCACAGTG GCTTGGTGCA AGAAGTGGGG TGTCCCAATT
    GAGAAGATTT ACAACAAAAC CCAGCGGGAG AAGTTTGCCT GGGCCATTGA CATGGCTGAT
    GAAGACTATG AGTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27246
    Clone ID Related Accession (Same CDS sequence) NM_003286.4 , NM_003286.3 , NM_003286.2
    Accession Version NM_003286.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2298bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-14
    Organism Homo sapiens(Human)
    Product DNA topoisomerase 1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGTGGGG ACCACCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
    TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCACAAAGA ACACAAGAAG
    GAGAAGGACC GGGAAAAGTC CAAGCATAGC AACAGTGAAC ATAAAGATTC TGAAAAGAAA
    CACAAAGAGA AGGAGAAGAC CAAACACAAA GATGGAAGCT CAGAAAAGCA TAAAGACAAA
    CATAAAGACA GAGACAAGGA AAAACGAAAA GAGGAAAAGG TTCGAGCCTC TGGGGATGCA
    AAAATAAAGA AGGAGAAGGA AAATGGCTTC TCTAGTCCAC CACAAATTAA AGATGAACCT
    GAAGATGATG GCTATTTTGT TCCTCCTAAA GAGGATATAA AGCCATTAAA GAGACCTCGA
    GATGAGGATG ATGCTGATTA TAAACCTAAG AAAATTAAAA CAGAAGATAC CAAGAAGGAG
    AAGAAAAGAA AACTAGAAGA AGAAGAGGAT GGTAAATTGA AAAAACCCAA GAATAAAGAT
    AAAGATAAAA AAGTTCCTGA GCCAGATAAC AAGAAAAAGA AGCCGAAGAA AGAAGAGGAA
    CAGAAGTGGA AATGGTGGGA AGAAGAGCGC TATCCTGAAG GCATCAAGTG GAAATTCCTA
    GAACATAAAG GTCCAGTATT TGCCCCACCA TATGAGCCTC TTCCAGAGAA TGTCAAGTTT
    TATTATGATG GTAAAGTCAT GAAGCTGAGC CCCAAAGCAG AGGAAGTAGC TACGTTCTTT
    GCAAAAATGC TCGACCATGA ATATACTACC AAGGAAATAT TTAGGAAAAA TTTCTTTAAA
    GACTGGAGAA AGGAAATGAC TAATGAAGAG AAGAATATTA TCACCAACCT AAGCAAATGT
    GATTTTACCC AGATGAGCCA GTATTTCAAA GCCCAGACGG AAGCTCGGAA ACAGATGAGC
    AAGGAAGAGA AACTGAAAAT CAAAGAGGAG AATGAAAAAT TACTGAAAGA ATATGGATTC
    TGTATTATGG ATAACCACAA AGAGAGGATT GCTAACTTCA AGATAGAGCC TCCTGGACTT
    TTCCGTGGCC GCGGCAACCA CCCCAAGATG GGCATGCTGA AGAGACGAAT CATGCCCGAG
    GATATAATCA TCAACTGTAG CAAAGATGCC AAGGTTCCTT CTCCTCCTCC AGGACATAAG
    TGGAAAGAAG TCCGGCATGA TAACAAGGTT ACTTGGCTGG TTTCCTGGAC AGAGAACATC
    CAAGGTTCCA TTAAATACAT CATGCTTAAC CCTAGTTCAC GAATCAAGGG TGAGAAGGAC
    TGGCAGAAAT ACGAGACTGC TCGGCGGCTG AAAAAATGTG TGGACAAGAT CCGGAACCAG
    TATCGAGAAG ACTGGAAGTC CAAAGAGATG AAAGTCCGGC AGAGAGCTGT AGCCCTGTAC
    TTCATCGACA AGCTTGCTCT GAGAGCAGGC AATGAAAAGG AGGAAGGAGA AACAGCGGAC
    ACTGTGGGCT GCTGCTCACT TCGTGTGGAG CACATCAATC TACACCCAGA GTTGGATGGT
    CAGGAATATG TGGTAGAGTT TGACTTCCTC GGGAAGGACT CCATCAGATA CTATAACAAG
    GTCCCTGTTG AGAAACGAGT TTTTAAGAAC CTACAACTAT TTATGGAGAA CAAGCAGCCC
    GAGGATGATC TTTTTGATAG ACTCAATACT GGTATTCTGA ATAAGCATCT TCAGGATCTC
    ATGGAGGGCT TGACAGCCAA GGTATTCCGT ACATACAATG CCTCCATCAC GCTACAGCAG
    CAGCTAAAAG AACTGACAGC CCCGGATGAG AACATCCCAG CGAAGATCCT TTCTTATAAC
    CGTGCCAATC GAGCTGTTGC AATTCTTTGT AACCATCAGA GGGCACCACC AAAAACTTTT
    GAGAAGTCTA TGATGAACTT GCAAACTAAG ATTGATGCCA AGAAGGAACA GCTAGCAGAT
    GCCCGGAGAG ACCTGAAAAG TGCTAAGGCT GATGCCAAGG TCATGAAGGA TGCAAAGACG
    AAGAAGGTAG TAGAGTCAAA GAAGAAGGCT GTTCAGAGAC TGGAGGAACA GTTGATGAAG
    CTGGAAGTTC AAGCCACAGA CCGAGAGGAA AATAAACAGA TTGCCCTGGG AACCTCCAAA
    CTCAATTATC TGGACCCTAG GATCACAGTG GCTTGGTGCA AGAAGTGGGG TGTCCCAATT
    GAGAAGATTT ACAACAAAAC CCAGCGGGAG AAGTTTGCCT GGGCCATTGA CATGGCTGAT
    GAAGACTATG AGTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003277.1
    Translation

    Target ORF information:

    RefSeq Version NM_003286.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens topoisomerase (DNA) I (TOP1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003286.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    ATGAGTGGGG ACCACCTCCA CAACGATTCC CAGATCGAAG CGGATTTCCG ATTGAATGAT 
    TCTCATAAAC ACAAAGATAA ACACAAAGAT CGAGAACACC GGCACAAAGA ACACAAGAAG
    GAGAAGGACC GGGAAAAGTC CAAGCATAGC AACAGTGAAC ATAAAGATTC TGAAAAGAAA
    CACAAAGAGA AGGAGAAGAC CAAACACAAA GATGGAAGCT CAGAAAAGCA TAAAGACAAA
    CATAAAGACA GAGACAAGGA AAAACGAAAA GAGGAAAAGG TTCGAGCCTC TGGGGATGCA
    AAAATAAAGA AGGAGAAGGA AAATGGCTTC TCTAGTCCAC CACAAATTAA AGATGAACCT
    GAAGATGATG GCTATTTTGT TCCTCCTAAA GAGGATATAA AGCCATTAAA GAGACCTCGA
    GATGAGGATG ATGCTGATTA TAAACCTAAG AAAATTAAAA CAGAAGATAC CAAGAAGGAG
    AAGAAAAGAA AACTAGAAGA AGAAGAGGAT GGTAAATTGA AAAAACCCAA GAATAAAGAT
    AAAGATAAAA AAGTTCCTGA GCCAGATAAC AAGAAAAAGA AGCCGAAGAA AGAAGAGGAA
    CAGAAGTGGA AATGGTGGGA AGAAGAGCGC TATCCTGAAG GCATCAAGTG GAAATTCCTA
    GAACATAAAG GTCCAGTATT TGCCCCACCA TATGAGCCTC TTCCAGAGAA TGTCAAGTTT
    TATTATGATG GTAAAGTCAT GAAGCTGAGC CCCAAAGCAG AGGAAGTAGC TACGTTCTTT
    GCAAAAATGC TCGACCATGA ATATACTACC AAGGAAATAT TTAGGAAAAA TTTCTTTAAA
    GACTGGAGAA AGGAAATGAC TAATGAAGAG AAGAATATTA TCACCAACCT AAGCAAATGT
    GATTTTACCC AGATGAGCCA GTATTTCAAA GCCCAGACGG AAGCTCGGAA ACAGATGAGC
    AAGGAAGAGA AACTGAAAAT CAAAGAGGAG AATGAAAAAT TACTGAAAGA ATATGGATTC
    TGTATTATGG ATAACCACAA AGAGAGGATT GCTAACTTCA AGATAGAGCC TCCTGGACTT
    TTCCGTGGCC GCGGCAACCA CCCCAAGATG GGCATGCTGA AGAGACGAAT CATGCCCGAG
    GATATAATCA TCAACTGTAG CAAAGATGCC AAGGTTCCTT CTCCTCCTCC AGGACATAAG
    TGGAAAGAAG TCCGGCATGA TAACAAGGTT ACTTGGCTGG TTTCCTGGAC AGAGAACATC
    CAAGGTTCCA TTAAATACAT CATGCTTAAC CCTAGTTCAC GAATCAAGGG TGAGAAGGAC
    TGGCAGAAAT ACGAGACTGC TCGGCGGCTG AAAAAATGTG TGGACAAGAT CCGGAACCAG
    TATCGAGAAG ACTGGAAGTC CAAAGAGATG AAAGTCCGGC AGAGAGCTGT AGCCCTGTAC
    TTCATCGACA AGCTTGCTCT GAGAGCAGGC AATGAAAAGG AGGAAGGAGA AACAGCGGAC
    ACTGTGGGCT GCTGCTCACT TCGTGTGGAG CACATCAATC TACACCCAGA GTTGGATGGT
    CAGGAATATG TGGTAGAGTT TGACTTCCTC GGGAAGGACT CCATCAGATA CTATAACAAG
    GTCCCTGTTG AGAAACGAGT TTTTAAGAAC CTACAACTAT TTATGGAGAA CAAGCAGCCC
    GAGGATGATC TTTTTGATAG ACTCAATACT GGTATTCTGA ATAAGCATCT TCAGGATCTC
    ATGGAGGGCT TGACAGCCAA GGTATTCCGT ACATACAATG CCTCCATCAC GCTACAGCAG
    CAGCTAAAAG AACTGACAGC CCCGGATGAG AACATCCCAG CGAAGATCCT TTCTTATAAC
    CGTGCCAATC GAGCTGTTGC AATTCTTTGT AACCATCAGA GGGCACCACC AAAAACTTTT
    GAGAAGTCTA TGATGAACTT GCAAACTAAG ATTGATGCCA AGAAGGAACA GCTAGCAGAT
    GCCCGGAGAG ACCTGAAAAG TGCTAAGGCT GATGCCAAGG TCATGAAGGA TGCAAAGACG
    AAGAAGGTAG TAGAGTCAAA GAAGAAGGCT GTTCAGAGAC TGGAGGAACA GTTGATGAAG
    CTGGAAGTTC AAGCCACAGA CCGAGAGGAA AATAAACAGA TTGCCCTGGG AACCTCCAAA
    CTCAATTATC TGGACCCTAG GATCACAGTG GCTTGGTGCA AGAAGTGGGG TGTCCCAATT
    GAGAAGATTT ACAACAAAAC CCAGCGGGAG AAGTTTGCCT GGGCCATTGA CATGGCTGAT
    GAAGACTATG AGTTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.