TJP1 cDNA ORF clone, Homo sapiens(human)

The following TJP1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TJP1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu25467 NM_003257.5
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$679.50-$951.30
$1359.00
OHu25544 NM_175610.4
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu85640 XM_017022531.1
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu85637 NM_001355013.1
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant 7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu85638 NM_001355014.2
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu85639 NM_001355015.2
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant 9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu85632 XM_017022525.1
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu85633 XM_017022526.1
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu85634 XM_017022527.1
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu85637 XM_017022528.1
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu85638 XM_017022529.1
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu85639 XM_017022530.1
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X15, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu48519 NM_001301025.1
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu48522 NM_001330239.3
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu48525 NM_001301026.2
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu102941 NM_001301025.3
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu85630 NM_001355012.2
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu85626 XM_017022521.1
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu85627 XM_017022522.1
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu85628 XM_017022523.1
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu85629 XM_011521972.2
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu85630 XM_005254617.3
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu85631 XM_017022524.1
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu85635 XM_005254619.3
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu85636 XM_005254620.3
Latest version!
Homo sapiens tight junction protein 1 (TJP1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
View Old Accession Versions >>
OHu102941 NM_001301025.2 Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu48525 NM_001301026.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu25544 NM_175610.3 Homo sapiens tight junction protein 1 (TJP1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$659.50-$923.30
$1319.00
OHu48522 NM_001330239.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu48522 NM_001330239.2 Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu85638 NM_001355014.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
OHu25467 NM_003257.4 Homo sapiens tight junction protein 1 (TJP1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$679.50-$951.30
$1359.00
OHu85630 NM_001355012.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $951.30
$1359.00
OHu85639 NM_001355015.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $951.30
$1359.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu25467
    Clone ID Related Accession (Same CDS sequence) NM_003257.4 , NM_003257.5
    Accession Version NM_003257.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-23
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AC022613.13, BX640879.1 and AF035298.1. On Jul 18, 2014 this sequence version replaced NM_003257.3. Transcript Variant: This variant (1, also known as ZO-1alpha+) encodes isoform a, which is found in most epithelial cell junctions. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC111712.1, AK304758.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003248.3
    CDS476..5722
    Translation

    Target ORF information:

    RefSeq Version NM_003257.4
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003257.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25467
    Clone ID Related Accession (Same CDS sequence) NM_003257.4 , NM_003257.5
    Accession Version NM_003257.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5247bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AC022613.13, BX640879.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_003257.4. Transcript Variant: This variant (1, also known as ZO-1alpha+) encodes isoform a, which is found in most epithelial cell junctions. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC111712.1, AK304758.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003248.3
    CDS441..5687
    Translation

    Target ORF information:

    RefSeq Version NM_003257.5
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003257.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25544
    Clone ID Related Accession (Same CDS sequence) NM_175610.3 , NM_175610.4
    Accession Version NM_175610.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5007bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-12
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AK302971.1, AC022613.13, BX640879.1 and AF035298.1. On Jul 18, 2014 this sequence version replaced NM_175610.2. Transcript Variant: This variant (2, also known as ZO-1alpha-) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Isoform b is found in both endothelial cells and the highly specialized epithelial junctions of renal glomeruli and sertoli cells of the seminiferous tubules. The exon combination of this variant is inferred based on partial transcript alignments and data in PMID:1590354. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.267789.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_783297.2
    CDS476..5482
    Translation

    Target ORF information:

    RefSeq Version NM_175610.3
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_175610.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25544
    Clone ID Related Accession (Same CDS sequence) NM_175610.3 , NM_175610.4
    Accession Version NM_175610.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5007bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AK302971.1, AC022613.13, BX640879.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_175610.3. Transcript Variant: This variant (2, also known as ZO-1alpha-) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Isoform b is found in both endothelial cells and the highly specialized epithelial junctions of renal glomeruli and sertoli cells of the seminiferous tubules. The exon combination of this variant is inferred based on partial transcript alignments and data in PMID:1590354. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.267789.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_783297.2
    CDS441..5447
    Translation

    Target ORF information:

    RefSeq Version NM_175610.4
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_175610.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85640
    Clone ID Related Accession (Same CDS sequence) XM_017022531.1
    Accession Version XM_017022531.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGACACACA GTCTCCGGAG TAGAGATGAA GAGAGAATTT CTAAACCTGG GGCTGTCTCA 
    ACTCCTGTAA AGCATGCTGA TGATCACACA CCTAAAACAG TGGAAGAAGT TACAGTTGAA
    AGAAATGAGA AACAAACACC TTCTCTTCCA GAACCAAAGC CTGTGTATGC CCAAGTTGGG
    CAACCAGATG TGGATTTACC TGTCAGTCCA TCTGATGGTG TCCTACCTAA TTCAACTCAT
    GAAGATGGGA TTCTTCGGCC CAGCATGAAA TTGGTAAAAT TCAGAAAAGG AGATAGTGTG
    GGTTTGCGGC TGGCTGGTGG AAATGATGTT GGAATATTTG TAGCTGGCGT TCTAGAAGAT
    AGCCCTGCAG CCAAGGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT AAACAACGTA
    GATTTTACAA ATATCATAAG AGAAGAAGCC GTCCTTTTCC TGCTTGACCT CCCTAAAGGA
    GAAGAAGTGA CCATATTGGC TCAGAAGAAG AAGGATGTTT ATCGTCGCAT TGTAGAATCA
    GATGTAGGAG ATTCTTTCTA TATTAGAACC CATTTTGAAT ATGAAAAGGA ATCTCCCTAT
    GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGTGTTGTGG ATACCTTGTA CAATGGAAAA
    CTGGGCTCTT GGCTTGCTAT TCGAATTGGT AAAAATCATA AGGAGGTAGA ACGAGGCATC
    ATCCCTAATA AGAACAGAGC TGAGCAGCTA GCCAGTGTAC AGTATACACT TCCAAAAACA
    GCAGGCGGAG ACCGTGCTGA CTTCTGGAGA TTCAGAGGTC TTCGCAGCTC CAAGAGAAAT
    CTTCGAAAAA GCAGAGAGGA TTTGTCCGCT CAGCCTGTTC AAACAAAGTT TCCAGCTTAT
    GAAAGAGTGG TTCTTCGAGA AGCTGGATTT CTGAGGCCTG TAACCATTTT TGGACCAATA
    GCTGATGTTG CCAGAGAAAA GCTGGCAAGA GAAGAACCAG ATATTTATCA AATTGCAAAG
    AGTGAACCAC GAGACGCTGG AACTGACCAA CGTAGCTCTG GCATTATTCG CCTGCATACA
    ATAAAGCAAA TCATAGATCA AGACAAACAT GCTTTATTAG ATGTAACACC AAATGCAGTT
    GATCGTCTTA ACTATGCCCA GTGGTATCCA ATTGTTGTAT TTCTTAACCC TGATTCTAAG
    CAAGGAGTAA AAACAATGAG AATGAGGTTA TGTCCAGAAT CTCGGAAAAG TGCCAGGAAG
    TTATACGAGC GATCTCATAA ACTTCGTAAA AATAATCACC ATCTTTTTAC AACTACAATT
    AACTTAAATT CAATGAATGA TGGTTGGTAT GGTGCGCTGA AAGAAGCAAT TCAACAACAG
    CAAAACCAGC TGGTATGGGT TTCCGAGGGA AAGGCGGATG GTGCTACAAG TGATGACCTT
    GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCTCCAG GTAGTGAATA CTCAATGTAT
    AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ACACAGAAGG CGGGGCCTAC
    ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTTG GGACTCCACC GGAGTCTGCC
    ATTACACGGT CCTCTGAGCC TGTAAGAGAG GACTCCTCTG GAATGCATCA TGAAAACCAA
    ACATATCCTC CTTACTCACC ACAAGCGCAG CCACAACCAA TTCATAGAAT AGACTCCCCT
    GGATTTAAGC CAGCCTCTCA ACAGAAAGCA GAAGCTTCAT CTCCAGTCCC TTACCTTTCG
    CCTGAAACAA ACCCAGCATC ATCAACCTCT GCTGTTAATC ATAATGTAAA TTTAACTAAT
    GTCAGACTGG AGGAGCCCAC CCCAGCTCCT TCCACCTCTT ACTCACCACA AGCTGATTCT
    TTAAGAACAC CAAGTACTGA GGCAGCTCAC ATAATGCTAA GAGATCAAGA ACCATCATTG
    TCGTCGCATG TAGATCCAAC AAAGGTGTAT AGAAAGGATC CATATCCCGA GGAAATGATG
    AGGCAGAACC ATGTTTTGAA ACAGCCAGCC GTTAGTCACC CAGGGCACAG GCCAGACAAA
    GAGCCTAATC TGACCTATGA ACCCCAACTC CCATACGTAG AGAAACAAGC CAGCAGAGAC
    CTCGAGCAGC CCACATACAG ATACGAGTCC TCAAGCTATA CGGACCAGTT TTCTCGAAAC
    TATGAACATC GTCTGCGATA CGAAGATCGC GTCCCCATGT ATGAAGAACA GTGGTCATAT
    TATGATGACA AACAGCCCTA CCCATCTCGG CCACCTTTTG ATAATCAGCA CTCTCAAGAC
    CTTGACTCCA GACAGCATCC CGAAGAGTCC TCAGAACGAG GGTACTTTCC ACGTTTTGAA
    GAGCCAGCCC CTCTGTCTTA CGACAGCAGA CCACGTTACG AACAGGCACC TAGAGCATCC
    GCCCTGCGGC ACGAAGAGCA GCCAGCTCCT GGGTATGACA CACATGGTAG ACTCAGACCG
    GAAGCCCAGC CCCACCCTTC AGCAGGGCCC AAGCCTGCAG AGTCCAAGCA GTATTTTGAG
    CAATATTCAC GCAGTTACGA GCAAGTACCA CCCCAAGGAT TTACCTCTAG AGCAGGTCAT
    TTTGAGCCTC TCCATGGTGC TGCAGCTGTC CCTCCGCTGA TACCTTCATC TCAGCATAAG
    CCAGAAGCTC TGCCTTCAAA CACCAAACCA CTGCCTCCAC CCCCAACTCA AACCGAAGAA
    GAGGAAGATC CAGCAATGAA GCCACAGTCT GTACTCACCA GAGTTAAGAT GTTTGAAAAC
    AAAAGATCTG CATCCTTAGA GACCAAGAAG GATGTAAATG ACACTGGCAG TTTTAAGCCT
    CCAGAAGTAG CATCTAAACC TTCAGGTGCT CCCATCATTG GTCCCAAACC CACTTCTCAG
    AATCAATTCA GTGAACATGA CAAAACTCTG TACAGGATCC CAGAACCTCA AAAACCTCAA
    CTGAAGCCAC CTGAAGATAT TGTTCGGTCC AATCATTATG ACCCTGAAGA AGATGAAGAA
    TATTATCGAA AACAGCTGTC ATACTTTGAC CGAAGAAGTT TTGAGAATAA GCCTCCTGCA
    CACATTGCCG CCAGCCATCT CTCCGAGCCT GCAAAGCCAG CGCATTCTCA GAATCAATCA
    AATTTTTCTA GTTATTCTTC AAAATTTCTT GGCTCTTACA CTAGCTATGA CTACTTGAAA
    AGGAACGTCA AGTGGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878020.1
    CDS1189..5547
    Translation

    Target ORF information:

    RefSeq Version XM_017022531.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022531.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGACACACA GTCTCCGGAG TAGAGATGAA GAGAGAATTT CTAAACCTGG GGCTGTCTCA 
    ACTCCTGTAA AGCATGCTGA TGATCACACA CCTAAAACAG TGGAAGAAGT TACAGTTGAA
    AGAAATGAGA AACAAACACC TTCTCTTCCA GAACCAAAGC CTGTGTATGC CCAAGTTGGG
    CAACCAGATG TGGATTTACC TGTCAGTCCA TCTGATGGTG TCCTACCTAA TTCAACTCAT
    GAAGATGGGA TTCTTCGGCC CAGCATGAAA TTGGTAAAAT TCAGAAAAGG AGATAGTGTG
    GGTTTGCGGC TGGCTGGTGG AAATGATGTT GGAATATTTG TAGCTGGCGT TCTAGAAGAT
    AGCCCTGCAG CCAAGGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT AAACAACGTA
    GATTTTACAA ATATCATAAG AGAAGAAGCC GTCCTTTTCC TGCTTGACCT CCCTAAAGGA
    GAAGAAGTGA CCATATTGGC TCAGAAGAAG AAGGATGTTT ATCGTCGCAT TGTAGAATCA
    GATGTAGGAG ATTCTTTCTA TATTAGAACC CATTTTGAAT ATGAAAAGGA ATCTCCCTAT
    GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGTGTTGTGG ATACCTTGTA CAATGGAAAA
    CTGGGCTCTT GGCTTGCTAT TCGAATTGGT AAAAATCATA AGGAGGTAGA ACGAGGCATC
    ATCCCTAATA AGAACAGAGC TGAGCAGCTA GCCAGTGTAC AGTATACACT TCCAAAAACA
    GCAGGCGGAG ACCGTGCTGA CTTCTGGAGA TTCAGAGGTC TTCGCAGCTC CAAGAGAAAT
    CTTCGAAAAA GCAGAGAGGA TTTGTCCGCT CAGCCTGTTC AAACAAAGTT TCCAGCTTAT
    GAAAGAGTGG TTCTTCGAGA AGCTGGATTT CTGAGGCCTG TAACCATTTT TGGACCAATA
    GCTGATGTTG CCAGAGAAAA GCTGGCAAGA GAAGAACCAG ATATTTATCA AATTGCAAAG
    AGTGAACCAC GAGACGCTGG AACTGACCAA CGTAGCTCTG GCATTATTCG CCTGCATACA
    ATAAAGCAAA TCATAGATCA AGACAAACAT GCTTTATTAG ATGTAACACC AAATGCAGTT
    GATCGTCTTA ACTATGCCCA GTGGTATCCA ATTGTTGTAT TTCTTAACCC TGATTCTAAG
    CAAGGAGTAA AAACAATGAG AATGAGGTTA TGTCCAGAAT CTCGGAAAAG TGCCAGGAAG
    TTATACGAGC GATCTCATAA ACTTCGTAAA AATAATCACC ATCTTTTTAC AACTACAATT
    AACTTAAATT CAATGAATGA TGGTTGGTAT GGTGCGCTGA AAGAAGCAAT TCAACAACAG
    CAAAACCAGC TGGTATGGGT TTCCGAGGGA AAGGCGGATG GTGCTACAAG TGATGACCTT
    GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCTCCAG GTAGTGAATA CTCAATGTAT
    AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ACACAGAAGG CGGGGCCTAC
    ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTTG GGACTCCACC GGAGTCTGCC
    ATTACACGGT CCTCTGAGCC TGTAAGAGAG GACTCCTCTG GAATGCATCA TGAAAACCAA
    ACATATCCTC CTTACTCACC ACAAGCGCAG CCACAACCAA TTCATAGAAT AGACTCCCCT
    GGATTTAAGC CAGCCTCTCA ACAGAAAGCA GAAGCTTCAT CTCCAGTCCC TTACCTTTCG
    CCTGAAACAA ACCCAGCATC ATCAACCTCT GCTGTTAATC ATAATGTAAA TTTAACTAAT
    GTCAGACTGG AGGAGCCCAC CCCAGCTCCT TCCACCTCTT ACTCACCACA AGCTGATTCT
    TTAAGAACAC CAAGTACTGA GGCAGCTCAC ATAATGCTAA GAGATCAAGA ACCATCATTG
    TCGTCGCATG TAGATCCAAC AAAGGTGTAT AGAAAGGATC CATATCCCGA GGAAATGATG
    AGGCAGAACC ATGTTTTGAA ACAGCCAGCC GTTAGTCACC CAGGGCACAG GCCAGACAAA
    GAGCCTAATC TGACCTATGA ACCCCAACTC CCATACGTAG AGAAACAAGC CAGCAGAGAC
    CTCGAGCAGC CCACATACAG ATACGAGTCC TCAAGCTATA CGGACCAGTT TTCTCGAAAC
    TATGAACATC GTCTGCGATA CGAAGATCGC GTCCCCATGT ATGAAGAACA GTGGTCATAT
    TATGATGACA AACAGCCCTA CCCATCTCGG CCACCTTTTG ATAATCAGCA CTCTCAAGAC
    CTTGACTCCA GACAGCATCC CGAAGAGTCC TCAGAACGAG GGTACTTTCC ACGTTTTGAA
    GAGCCAGCCC CTCTGTCTTA CGACAGCAGA CCACGTTACG AACAGGCACC TAGAGCATCC
    GCCCTGCGGC ACGAAGAGCA GCCAGCTCCT GGGTATGACA CACATGGTAG ACTCAGACCG
    GAAGCCCAGC CCCACCCTTC AGCAGGGCCC AAGCCTGCAG AGTCCAAGCA GTATTTTGAG
    CAATATTCAC GCAGTTACGA GCAAGTACCA CCCCAAGGAT TTACCTCTAG AGCAGGTCAT
    TTTGAGCCTC TCCATGGTGC TGCAGCTGTC CCTCCGCTGA TACCTTCATC TCAGCATAAG
    CCAGAAGCTC TGCCTTCAAA CACCAAACCA CTGCCTCCAC CCCCAACTCA AACCGAAGAA
    GAGGAAGATC CAGCAATGAA GCCACAGTCT GTACTCACCA GAGTTAAGAT GTTTGAAAAC
    AAAAGATCTG CATCCTTAGA GACCAAGAAG GATGTAAATG ACACTGGCAG TTTTAAGCCT
    CCAGAAGTAG CATCTAAACC TTCAGGTGCT CCCATCATTG GTCCCAAACC CACTTCTCAG
    AATCAATTCA GTGAACATGA CAAAACTCTG TACAGGATCC CAGAACCTCA AAAACCTCAA
    CTGAAGCCAC CTGAAGATAT TGTTCGGTCC AATCATTATG ACCCTGAAGA AGATGAAGAA
    TATTATCGAA AACAGCTGTC ATACTTTGAC CGAAGAAGTT TTGAGAATAA GCCTCCTGCA
    CACATTGCCG CCAGCCATCT CTCCGAGCCT GCAAAGCCAG CGCATTCTCA GAATCAATCA
    AATTTTTCTA GTTATTCTTC AAAATTTCTT GGCTCTTACA CTAGCTATGA CTACTTGAAA
    AGGAACGTCA AGTGGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85637
    Clone ID Related Accession (Same CDS sequence) XM_017022528.1 , NM_001355013.1
    Accession Version NM_001355013.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform g
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022613.13, BC111712.1, BU618228.1, BX640879.1 and AF035298.1. On Sep 1, 2017 this sequence version replaced XM_017022528.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1803612.77932.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA
    AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG
    GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA
    CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT
    GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC
    GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341942.1
    CDS110..5389
    Translation

    Target ORF information:

    RefSeq Version NM_001355013.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355013.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA
    AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG
    GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA
    CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT
    GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC
    GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85638
    Clone ID Related Accession (Same CDS sequence) XM_017022529.1 , NM_001355014.1 , NM_001355014.2
    Accession Version NM_001355014.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform h
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AC022613.13, BU618228.1, BX640879.1 and AF035298.1. On Sep 1, 2017 this sequence version replaced XM_017022529.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.97584.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT
    TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341943.1
    CDS476..5743
    Translation

    Target ORF information:

    RefSeq Version NM_001355014.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355014.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT
    TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85638
    Clone ID Related Accession (Same CDS sequence) XM_017022529.1 , NM_001355014.1 , NM_001355014.2
    Accession Version NM_001355014.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform h
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AC022613.13, BU618228.1, BX640879.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_001355014.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.97584.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT
    TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341943.1
    CDS441..5708
    Translation

    Target ORF information:

    RefSeq Version NM_001355014.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355014.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT
    TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85639
    Clone ID Related Accession (Same CDS sequence) XM_017022530.1 , NM_001355015.1 , NM_001355015.2
    Accession Version NM_001355015.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-12
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform i
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AK302971.1, AC022613.13, BM722482.1, BX640879.1 and AF035298.1. On Sep 1, 2017 this sequence version replaced XM_017022530.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.127.1, SRR1803614.6915.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341944.1
    CDS476..5542
    Translation

    Target ORF information:

    RefSeq Version NM_001355015.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85639
    Clone ID Related Accession (Same CDS sequence) XM_017022530.1 , NM_001355015.1 , NM_001355015.2
    Accession Version NM_001355015.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform i
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AK302971.1, AC022613.13, BM722482.1, BX640879.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_001355015.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.127.1, SRR1803614.260442.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341944.1
    CDS441..5507
    Translation

    Target ORF information:

    RefSeq Version NM_001355015.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355015.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85632
    Clone ID Related Accession (Same CDS sequence) XM_017022525.1
    Accession Version XM_017022525.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5370bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA
    AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG
    GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA
    CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT
    GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAATTTCT TGGCTCTTAC ACTAGCTATG ACTACTTGAA AAGGAACGTC
    AAGTGGGGAA AGCCTCCTGA AGCTGATGGT GTGGATAGAT CATTTGGCGA GAAACGCTAT
    GAACCCATCC AGGCCACTCC CCCTCCTCCT CCATTGCCCT CGCAGTATGC CCAGCCATCT
    CAGCCTGTCA CCAGCGCGTC TCTCCACATA CATTCTAAGG GAGCACATGG TGAAGGTAAT
    TCAGTGTCAT TGGATTTTCA GAATTCCTTA GTGTCCAAAC CAGACCCACC TCCATCTCAG
    AATAAGCCAG CAACTTTCAG ACCACCAAAC CGAGAAGATA CTGCTCAGGC AGCTTTCTAT
    CCCCAGAAAA GTTTTCCAGA TAAAGCCCCA GTTAATGGAA CTGAACAGAC TCAGAAAACA
    GTCACTCCAG CATACAATCG ATTCACACCA AAACCATATA CAAGTTCTGC CCGACCATTT
    GAACGCAAGT TTGAAAGTCC TAAATTCAAT CACAATCTTC TGCCAAGTGA AACTGCACAT
    AAACCTGACT TGTCTTCAAA AACTCCCACT TCTCCAAAAA CTCTTGTGAA ATCGCACAGT
    TTGGCACAGC CTCCTGAGTT TGACAGTGGA GTTGAAACTT TCTCTATCCA TGCAGAGAAG
    CCTAAATATC AAATAAATAA TATCAGCACA GTGCCTAAAG CTATTCCTGT GAGTCCTTCA
    GCTGTGGAAG AGGATGAAGA TGAAGATGGT CATACTGTGG TGGCCACAGC CCGAGGCATA
    TTTAACAGCA ATGGGGGCGT GCTGAGTTCC ATAGAAACTG GTGTTAGTAT AATTATCCCT
    CAAGGAGCCA TTCCCGAAGG AGTTGAGCAG GAAATCTATT TCAAGGTCTG CCGGGACAAC
    AGCATCCTTC CACCTTTAGA TAAAGAGAAA GGTGAAACAC TGCTGAGTCC TTTGGTGATG
    TGTGGTCCCC ATGGCCTCAA GTTCCTGAAG CCTGTGGAGC TGCGCTTACC ACACTGTGCG
    TCCATGACTC CTGACGGTTG GTCTTTTGCT CTAAAATCAT CCGACTCCTC GTCGGGTGAT
    CCTAAAACCT GGCAAAACAA GTGTCTTCCC GGAGATCCAA ATTATCTCGT TGGAGCAAAC
    TGTGTTTCTG TCCTTATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878014.1
    CDS110..5479
    Translation

    Target ORF information:

    RefSeq Version XM_017022525.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022525.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA
    AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG
    GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA
    CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT
    GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAATTTCT TGGCTCTTAC ACTAGCTATG ACTACTTGAA AAGGAACGTC
    AAGTGGGGAA AGCCTCCTGA AGCTGATGGT GTGGATAGAT CATTTGGCGA GAAACGCTAT
    GAACCCATCC AGGCCACTCC CCCTCCTCCT CCATTGCCCT CGCAGTATGC CCAGCCATCT
    CAGCCTGTCA CCAGCGCGTC TCTCCACATA CATTCTAAGG GAGCACATGG TGAAGGTAAT
    TCAGTGTCAT TGGATTTTCA GAATTCCTTA GTGTCCAAAC CAGACCCACC TCCATCTCAG
    AATAAGCCAG CAACTTTCAG ACCACCAAAC CGAGAAGATA CTGCTCAGGC AGCTTTCTAT
    CCCCAGAAAA GTTTTCCAGA TAAAGCCCCA GTTAATGGAA CTGAACAGAC TCAGAAAACA
    GTCACTCCAG CATACAATCG ATTCACACCA AAACCATATA CAAGTTCTGC CCGACCATTT
    GAACGCAAGT TTGAAAGTCC TAAATTCAAT CACAATCTTC TGCCAAGTGA AACTGCACAT
    AAACCTGACT TGTCTTCAAA AACTCCCACT TCTCCAAAAA CTCTTGTGAA ATCGCACAGT
    TTGGCACAGC CTCCTGAGTT TGACAGTGGA GTTGAAACTT TCTCTATCCA TGCAGAGAAG
    CCTAAATATC AAATAAATAA TATCAGCACA GTGCCTAAAG CTATTCCTGT GAGTCCTTCA
    GCTGTGGAAG AGGATGAAGA TGAAGATGGT CATACTGTGG TGGCCACAGC CCGAGGCATA
    TTTAACAGCA ATGGGGGCGT GCTGAGTTCC ATAGAAACTG GTGTTAGTAT AATTATCCCT
    CAAGGAGCCA TTCCCGAAGG AGTTGAGCAG GAAATCTATT TCAAGGTCTG CCGGGACAAC
    AGCATCCTTC CACCTTTAGA TAAAGAGAAA GGTGAAACAC TGCTGAGTCC TTTGGTGATG
    TGTGGTCCCC ATGGCCTCAA GTTCCTGAAG CCTGTGGAGC TGCGCTTACC ACACTGTGCG
    TCCATGACTC CTGACGGTTG GTCTTTTGCT CTAAAATCAT CCGACTCCTC GTCGGGTGAT
    CCTAAAACCT GGCAAAACAA GTGTCTTCCC GGAGATCCAA ATTATCTCGT TGGAGCAAAC
    TGTGTTTCTG TCCTTATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85633
    Clone ID Related Accession (Same CDS sequence) XM_017022526.1
    Accession Version XM_017022526.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5358bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAATTTCTTG GCTCTTACAC TAGCTATGAC TACTTGAAAA GGAACGTCAA GTGGGGAAAG
    CCTCCTGAAG CTGATGGTGT GGATAGATCA TTTGGCGAGA AACGCTATGA ACCCATCCAG
    GCCACTCCCC CTCCTCCTCC ATTGCCCTCG CAGTATGCCC AGCCATCTCA GCCTGTCACC
    AGCGCGTCTC TCCACATACA TTCTAAGGGA GCACATGGTG AAGGTAATTC AGTGTCATTG
    GATTTTCAGA ATTCCTTAGT GTCCAAACCA GACCCACCTC CATCTCAGAA TAAGCCAGCA
    ACTTTCAGAC CACCAAACCG AGAAGATACT GCTCAGGCAG CTTTCTATCC CCAGAAAAGT
    TTTCCAGATA AAGCCCCAGT TAATGGAACT GAACAGACTC AGAAAACAGT CACTCCAGCA
    TACAATCGAT TCACACCAAA ACCATATACA AGTTCTGCCC GACCATTTGA ACGCAAGTTT
    GAAAGTCCTA AATTCAATCA CAATCTTCTG CCAAGTGAAA CTGCACATAA ACCTGACTTG
    TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTGTGAAAT CGCACAGTTT GGCACAGCCT
    CCTGAGTTTG ACAGTGGAGT TGAAACTTTC TCTATCCATG CAGAGAAGCC TAAATATCAA
    ATAAATAATA TCAGCACAGT GCCTAAAGCT ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG
    GATGAAGATG AAGATGGTCA TACTGTGGTG GCCACAGCCC GAGGCATATT TAACAGCAAT
    GGGGGCGTGC TGAGTTCCAT AGAAACTGGT GTTAGTATAA TTATCCCTCA AGGAGCCATT
    CCCGAAGGAG TTGAGCAGGA AATCTATTTC AAGGTCTGCC GGGACAACAG CATCCTTCCA
    CCTTTAGATA AAGAGAAAGG TGAAACACTG CTGAGTCCTT TGGTGATGTG TGGTCCCCAT
    GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG CGCTTACCAC ACTGTGCGTC CATGACTCCT
    GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG
    CAAAACAAGT GTCTTCCCGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC
    CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878015.1
    CDS107..5464
    Translation

    Target ORF information:

    RefSeq Version XM_017022526.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022526.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAATTTCTTG GCTCTTACAC TAGCTATGAC TACTTGAAAA GGAACGTCAA GTGGGGAAAG
    CCTCCTGAAG CTGATGGTGT GGATAGATCA TTTGGCGAGA AACGCTATGA ACCCATCCAG
    GCCACTCCCC CTCCTCCTCC ATTGCCCTCG CAGTATGCCC AGCCATCTCA GCCTGTCACC
    AGCGCGTCTC TCCACATACA TTCTAAGGGA GCACATGGTG AAGGTAATTC AGTGTCATTG
    GATTTTCAGA ATTCCTTAGT GTCCAAACCA GACCCACCTC CATCTCAGAA TAAGCCAGCA
    ACTTTCAGAC CACCAAACCG AGAAGATACT GCTCAGGCAG CTTTCTATCC CCAGAAAAGT
    TTTCCAGATA AAGCCCCAGT TAATGGAACT GAACAGACTC AGAAAACAGT CACTCCAGCA
    TACAATCGAT TCACACCAAA ACCATATACA AGTTCTGCCC GACCATTTGA ACGCAAGTTT
    GAAAGTCCTA AATTCAATCA CAATCTTCTG CCAAGTGAAA CTGCACATAA ACCTGACTTG
    TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTGTGAAAT CGCACAGTTT GGCACAGCCT
    CCTGAGTTTG ACAGTGGAGT TGAAACTTTC TCTATCCATG CAGAGAAGCC TAAATATCAA
    ATAAATAATA TCAGCACAGT GCCTAAAGCT ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG
    GATGAAGATG AAGATGGTCA TACTGTGGTG GCCACAGCCC GAGGCATATT TAACAGCAAT
    GGGGGCGTGC TGAGTTCCAT AGAAACTGGT GTTAGTATAA TTATCCCTCA AGGAGCCATT
    CCCGAAGGAG TTGAGCAGGA AATCTATTTC AAGGTCTGCC GGGACAACAG CATCCTTCCA
    CCTTTAGATA AAGAGAAAGG TGAAACACTG CTGAGTCCTT TGGTGATGTG TGGTCCCCAT
    GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG CGCTTACCAC ACTGTGCGTC CATGACTCCT
    GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG
    CAAAACAAGT GTCTTCCCGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC
    CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85634
    Clone ID Related Accession (Same CDS sequence) XM_017022527.1
    Accession Version XM_017022527.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5352bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTGTATT ACTTATTTAA GAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
    ACAGTGACGC TTCACAGGGC TCCTGGATTT GGATTTGGAA TTGCAATATC TGGTGGACGA
    GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA
    GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA
    ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA
    AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA
    CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC
    CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT
    AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCTGCT
    AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG
    GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT
    ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG
    ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA
    GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT
    GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA
    TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT
    TCTGATCATT CCAGGCACTC GCCGCAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT
    GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC
    ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT
    CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAACCAG ATGTGGATTT ACCTGTCAGT
    CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG
    AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT
    GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG
    GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA
    GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG
    AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA
    ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG
    TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT
    GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG
    CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG
    AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC
    GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA
    TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA
    AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC
    CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA
    CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT
    CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG
    TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATACG AGCGATCTCA TAAACTTCGT
    AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG
    TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG
    GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC
    CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC
    TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT
    AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA
    GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG
    CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA
    GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC
    TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT
    CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT
    CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG
    TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA
    GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA
    CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG
    TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT
    CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT
    CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG
    TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC
    AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT
    CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG
    CCCAAGCCTG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA
    CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT
    GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA
    CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG
    TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG
    AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT
    GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAAT TCAGTGAACA TGACAAAACT
    CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATTGTTCGG
    TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT
    GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG
    CCTGCAAAGC CAGCGCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAATTT
    CTTGGCTCTT ACACTAGCTA TGACTACTTG AAAAGGAACG TCAAGTGGGG AAAGCCTCCT
    GAAGCTGATG GTGTGGATAG ATCATTTGGC GAGAAACGCT ATGAACCCAT CCAGGCCACT
    CCCCCTCCTC CTCCATTGCC CTCGCAGTAT GCCCAGCCAT CTCAGCCTGT CACCAGCGCG
    TCTCTCCACA TACATTCTAA GGGAGCACAT GGTGAAGGTA ATTCAGTGTC ATTGGATTTT
    CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC
    AGACCACCAA ACCGAGAAGA TACTGCTCAG GCAGCTTTCT ATCCCCAGAA AAGTTTTCCA
    GATAAAGCCC CAGTTAATGG AACTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT
    CGATTCACAC CAAAACCATA TACAAGTTCT GCCCGACCAT TTGAACGCAA GTTTGAAAGT
    CCTAAATTCA ATCACAATCT TCTGCCAAGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA
    AAAACTCCCA CTTCTCCAAA AACTCTTGTG AAATCGCACA GTTTGGCACA GCCTCCTGAG
    TTTGACAGTG GAGTTGAAAC TTTCTCTATC CATGCAGAGA AGCCTAAATA TCAAATAAAT
    AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTGTGGA AGAGGATGAA
    GATGAAGATG GTCATACTGT GGTGGCCACA GCCCGAGGCA TATTTAACAG CAATGGGGGC
    GTGCTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CTCAAGGAGC CATTCCCGAA
    GGAGTTGAGC AGGAAATCTA TTTCAAGGTC TGCCGGGACA ACAGCATCCT TCCACCTTTA
    GATAAAGAGA AAGGTGAAAC ACTGCTGAGT CCTTTGGTGA TGTGTGGTCC CCATGGCCTC
    AAGTTCCTGA AGCCTGTGGA GCTGCGCTTA CCACACTGTG CGTCCATGAC TCCTGACGGT
    TGGTCTTTTG CTCTAAAATC ATCCGACTCC TCGTCGGGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CCGGAGATCC AAATTATCTC GTTGGAGCAA ACTGTGTTTC TGTCCTTATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878016.1
    CDS154..5505
    Translation

    Target ORF information:

    RefSeq Version XM_017022527.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022527.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCTGTATT ACTTATTTAA GAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
    ACAGTGACGC TTCACAGGGC TCCTGGATTT GGATTTGGAA TTGCAATATC TGGTGGACGA
    GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA
    GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA
    ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA
    AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA
    CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC
    CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT
    AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCTGCT
    AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG
    GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT
    ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG
    ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA
    GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT
    GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA
    TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT
    TCTGATCATT CCAGGCACTC GCCGCAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT
    GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC
    ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT
    CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAACCAG ATGTGGATTT ACCTGTCAGT
    CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG
    AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT
    GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG
    GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA
    GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG
    AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA
    ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG
    TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT
    GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG
    CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG
    AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC
    GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA
    TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA
    AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC
    CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA
    CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT
    CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG
    TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATACG AGCGATCTCA TAAACTTCGT
    AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG
    TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG
    GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC
    CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC
    TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT
    AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA
    GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG
    CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA
    GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC
    TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT
    CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT
    CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG
    TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA
    GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA
    CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG
    TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT
    CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT
    CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG
    TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC
    AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT
    CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG
    CCCAAGCCTG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA
    CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT
    GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA
    CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG
    TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG
    AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT
    GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAAT TCAGTGAACA TGACAAAACT
    CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATTGTTCGG
    TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT
    GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG
    CCTGCAAAGC CAGCGCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAATTT
    CTTGGCTCTT ACACTAGCTA TGACTACTTG AAAAGGAACG TCAAGTGGGG AAAGCCTCCT
    GAAGCTGATG GTGTGGATAG ATCATTTGGC GAGAAACGCT ATGAACCCAT CCAGGCCACT
    CCCCCTCCTC CTCCATTGCC CTCGCAGTAT GCCCAGCCAT CTCAGCCTGT CACCAGCGCG
    TCTCTCCACA TACATTCTAA GGGAGCACAT GGTGAAGGTA ATTCAGTGTC ATTGGATTTT
    CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC
    AGACCACCAA ACCGAGAAGA TACTGCTCAG GCAGCTTTCT ATCCCCAGAA AAGTTTTCCA
    GATAAAGCCC CAGTTAATGG AACTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT
    CGATTCACAC CAAAACCATA TACAAGTTCT GCCCGACCAT TTGAACGCAA GTTTGAAAGT
    CCTAAATTCA ATCACAATCT TCTGCCAAGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA
    AAAACTCCCA CTTCTCCAAA AACTCTTGTG AAATCGCACA GTTTGGCACA GCCTCCTGAG
    TTTGACAGTG GAGTTGAAAC TTTCTCTATC CATGCAGAGA AGCCTAAATA TCAAATAAAT
    AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTGTGGA AGAGGATGAA
    GATGAAGATG GTCATACTGT GGTGGCCACA GCCCGAGGCA TATTTAACAG CAATGGGGGC
    GTGCTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CTCAAGGAGC CATTCCCGAA
    GGAGTTGAGC AGGAAATCTA TTTCAAGGTC TGCCGGGACA ACAGCATCCT TCCACCTTTA
    GATAAAGAGA AAGGTGAAAC ACTGCTGAGT CCTTTGGTGA TGTGTGGTCC CCATGGCCTC
    AAGTTCCTGA AGCCTGTGGA GCTGCGCTTA CCACACTGTG CGTCCATGAC TCCTGACGGT
    TGGTCTTTTG CTCTAAAATC ATCCGACTCC TCGTCGGGTG ATCCTAAAAC CTGGCAAAAC
    AAGTGTCTTC CCGGAGATCC AAATTATCTC GTTGGAGCAA ACTGTGTTTC TGTCCTTATT
    GACCACTTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85637
    Clone ID Related Accession (Same CDS sequence) XM_017022528.1 , NM_001355013.1
    Accession Version XM_017022528.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5280bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product tight junction protein ZO-1 isoform X13
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA
    AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG
    GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA
    CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT
    GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC
    GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878017.1
    CDS110..5389
    Translation

    Target ORF information:

    RefSeq Version XM_017022528.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022528.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA
    AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG
    GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA
    CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT
    GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC
    GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85638
    Clone ID Related Accession (Same CDS sequence) XM_017022529.1 , NM_001355014.1 , NM_001355014.2
    Accession Version XM_017022529.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5268bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product tight junction protein ZO-1 isoform X14
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT
    TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878018.1
    CDS107..5374
    Translation

    Target ORF information:

    RefSeq Version XM_017022529.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022529.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT
    TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85639
    Clone ID Related Accession (Same CDS sequence) XM_017022530.1 , NM_001355015.1 , NM_001355015.2
    Accession Version XM_017022530.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5067bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product tight junction protein ZO-1 isoform X15
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878019.1
    CDS107..5173
    Translation

    Target ORF information:

    RefSeq Version XM_017022530.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X15, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022530.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48519
    Clone ID Related Accession (Same CDS sequence) NM_001301025.1
    Accession Version NM_001301025.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5508bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-22
    Organism Homo sapiens(Human)
    Product tight junction protein ZO-1 isoform c
    Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN372462.1, AB370197.1, AC022613.13 and AF035298.1. On or before Jul 18, 2014 this sequence version replaced XM_006725553.1, XM_005254616.1. Transcript Variant: This variant (3) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, and it uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is longer than isoform a. The exon combination of this variant is inferred based on partial transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGTACC AGAAATACCT GACGGTGCTG CAGATGGCCA TCGGCGTCAC CCCCTCCAAC 
    CGCGGCAGCC TCCTGCCGCT CAAGAGGAAG CTGTGGGTAA CGCCATCCTC TGAAAATCCT
    AATGGTGCTA CTTCTAGTGT CAGCCAAGGA AAACCCTCTT TAAGACGAAT TAAAGGGAGA
    TTACACAGAA GCAAAAGCCT TGATAGCATG GATTTCTGTG AGCTCACTAG CACAGCAATG
    GAGGAAACAG CTATATGGGA ACAACATACA GTGACGCTTC ACAGGGCTCC TGGATTTGGA
    TTTGGAATTG CAATATCTGG TGGACGAGAT AATCCTCATT TTCAGAGTGG GGAAACGTCA
    ATAGTGATTT CAGATGTGCT GAAAGGAGGA CCAGCTGAAG GACAGCTACA GGAAAATGAC
    CGAGTTGCAA TGGTTAACGG AGTTTCAATG GATAATGTTG AACATGCTTT TGCTGTTCAG
    CAACTAAGGA AAAGTGGGAA AAATGCAAAA ATTACAATTA GAAGGAAGAA GAAAGTTCAA
    ATACCAGTAA GTCGTCCTGA TCCTGAACCA GTATCTGATA ATGAAGAAGA TAGTTATGAT
    GAGGAAATAC ATGATCCAAG AAGTGGCCGG AGTGGTGTGG TTAACAGAAG GAGTGAGAAG
    ATTTGGCCGA GGGATAGAAG TGCAAGTAGA GAGAGGAGCT TGTCCCCGCG GTCAGACAGG
    CGGTCAGTGG CTTCCAGCCA GCCTGCTAAA CCTACTAAAG TCACACTGGT GAAATCCCGG
    AAAAATGAAG AATATGGTCT TCGATTGGCA AGCCATATAT TTGTTAAGGA AATTTCACAA
    GATAGTTTGG CAGCAAGAGA TGGCAATATT CAAGAAGGTG ATGTTGTATT GAAGATAAAT
    GGTACTGTGA CAGAAAATAT GTCATTGACA GATGCAAAGA CATTGATAGA AAGGTCTAAA
    GGCAAATTAA AAATGGTAGT TCAAAGAGAT GAACGGGCTA CGCTATTGAA TGTCCCTGAT
    CTTTCTGACA GCATCCACTC TGCTAATGCC TCTGAGAGAG ACGACATTTC AGAAATTCAG
    TCACTGGCAT CAGATCATTC TGGTCGATCA CACGATAGGC CTCCCCGCCG CAGCCGGTCA
    CGATCTCCTG ACCAGCGGTC AGAGCCTTCT GATCATTCCA GGCACTCGCC GCAGCAGCCA
    AGCAATGGCA GTCTCCGGAG TAGAGATGAA GAGAGAATTT CTAAACCTGG GGCTGTCTCA
    ACTCCTGTAA AGCATGCTGA TGATCACACA CCTAAAACAG TGGAAGAAGT TACAGTTGAA
    AGAAATGAGA AACAAACACC TTCTCTTCCA GAACCAAAGC CTGTGTATGC CCAAGTTGGG
    CAACCAGATG TGGATTTACC TGTCAGTCCA TCTGATGGTG TCCTACCTAA TTCAACTCAT
    GAAGATGGGA TTCTTCGGCC CAGCATGAAA TTGGTAAAAT TCAGAAAAGG AGATAGTGTG
    GGTTTGCGGC TGGCTGGTGG AAATGATGTT GGAATATTTG TAGCTGGCGT TCTAGAAGAT
    AGCCCTGCAG CCAAGGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT AAACAACGTA
    GATTTTACAA ATATCATAAG AGAAGAAGCC GTCCTTTTCC TGCTTGACCT CCCTAAAGGA
    GAAGAAGTGA CCATATTGGC TCAGAAGAAG AAGGATGTTT ATCGTCGCAT TGTAGAATCA
    GATGTAGGAG ATTCTTTCTA TATTAGAACC CATTTTGAAT ATGAAAAGGA ATCTCCCTAT
    GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGTGTTGTGG ATACCTTGTA CAATGGAAAA
    CTGGGCTCTT GGCTTGCTAT TCGAATTGGT AAAAATCATA AGGAGGTAGA ACGAGGCATC
    ATCCCTAATA AGAACAGAGC TGAGCAGCTA GCCAGTGTAC AGTATACACT TCCAAAAACA
    GCAGGCGGAG ACCGTGCTGA CTTCTGGAGA TTCAGAGGTC TTCGCAGCTC CAAGAGAAAT
    CTTCGAAAAA GCAGAGAGGA TTTGTCCGCT CAGCCTGTTC AAACAAAGTT TCCAGCTTAT
    GAAAGAGTGG TTCTTCGAGA AGCTGGATTT CTGAGGCCTG TAACCATTTT TGGACCAATA
    GCTGATGTTG CCAGAGAAAA GCTGGCAAGA GAAGAACCAG ATATTTATCA AATTGCAAAG
    AGTGAACCAC GAGACGCTGG AACTGACCAA CGTAGCTCTG GCATTATTCG CCTGCATACA
    ATAAAGCAAA TCATAGATCA AGACAAACAT GCTTTATTAG ATGTAACACC AAATGCAGTT
    GATCGTCTTA ACTATGCCCA GTGGTATCCA ATTGTTGTAT TTCTTAACCC TGATTCTAAG
    CAAGGAGTAA AAACAATGAG AATGAGGTTA TGTCCAGAAT CTCGGAAAAG TGCCAGGAAG
    TTATACGAGC GATCTCATAA ACTTCGTAAA AATAATCACC ATCTTTTTAC AACTACAATT
    AACTTAAATT CAATGAATGA TGGTTGGTAT GGTGCGCTGA AAGAAGCAAT TCAACAACAG
    CAAAACCAGC TGGTATGGGT TTCCGAGGGA AAGGCGGATG GTGCTACAAG TGATGACCTT
    GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCTCCAG GTAGTGAATA CTCAATGTAT
    AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ACACAGAAGG CGGGGCCTAC
    ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTTG GGACTCCACC GGAGTCTGCC
    ATTACACGGT CCTCTGAGCC TGTAAGAGAG GACTCCTCTG GAATGCATCA TGAAAACCAA
    ACATATCCTC CTTACTCACC ACAAGCGCAG CCACAACCAA TTCATAGAAT AGACTCCCCT
    GGATTTAAGC CAGCCTCTCA ACAGAAAGCA GAAGCTTCAT CTCCAGTCCC TTACCTTTCG
    CCTGAAACAA ACCCAGCATC ATCAACCTCT GCTGTTAATC ATAATGTAAA TTTAACTAAT
    GTCAGACTGG AGGAGCCCAC CCCAGCTCCT TCCACCTCTT ACTCACCACA AGCTGATTCT
    TTAAGAACAC CAAGTACTGA GGCAGCTCAC ATAATGCTAA GAGATCAAGA ACCATCATTG
    TCGTCGCATG TAGATCCAAC AAAGGTGTAT AGAAAGGATC CATATCCCGA GGAAATGATG
    AGGCAGAACC ATGTTTTGAA ACAGCCAGCC GTTAGTCACC CAGGGCACAG GCCAGACAAA
    GAGCCTAATC TGACCTATGA ACCCCAACTC CCATACGTAG AGAAACAAGC CAGCAGAGAC
    CTCGAGCAGC CCACATACAG ATACGAGTCC TCAAGCTATA CGGACCAGTT TTCTCGAAAC
    TATGAACATC GTCTGCGATA CGAAGATCGC GTCCCCATGT ATGAAGAACA GTGGTCATAT
    TATGATGACA AACAGCCCTA CCCATCTCGG CCACCTTTTG ATAATCAGCA CTCTCAAGAC
    CTTGACTCCA GACAGCATCC CGAAGAGTCC TCAGAACGAG GGTACTTTCC ACGTTTTGAA
    GAGCCAGCCC CTCTGTCTTA CGACAGCAGA CCACGTTACG AACAGGCACC TAGAGCATCC
    GCCCTGCGGC ACGAAGAGCA GCCAGCTCCT GGGTATGACA CACATGGTAG ACTCAGACCG
    GAAGCCCAGC CCCACCCTTC AGCAGGGCCC AAGCCTGCAG AGTCCAAGCA GTATTTTGAG
    CAATATTCAC GCAGTTACGA GCAAGTACCA CCCCAAGGAT TTACCTCTAG AGCAGGTCAT
    TTTGAGCCTC TCCATGGTGC TGCAGCTGTC CCTCCGCTGA TACCTTCATC TCAGCATAAG
    CCAGAAGCTC TGCCTTCAAA CACCAAACCA CTGCCTCCAC CCCCAACTCA AACCGAAGAA
    GAGGAAGATC CAGCAATGAA GCCACAGTCT GTACTCACCA GAGTTAAGAT GTTTGAAAAC
    AAAAGATCTG CATCCTTAGA GACCAAGAAG GATGTAAATG ACACTGGCAG TTTTAAGCCT
    CCAGAAGTAG CATCTAAACC TTCAGGTGCT CCCATCATTG GTCCCAAACC CACTTCTCAG
    AATCAATTCA GTGAACATGA CAAAACTCTG TACAGGATCC CAGAACCTCA AAAACCTCAA
    CTGAAGCCAC CTGAAGATAT TGTTCGGTCC AATCATTATG ACCCTGAAGA AGATGAAGAA
    TATTATCGAA AACAGCTGTC ATACTTTGAC CGAAGAAGTT TTGAGAATAA GCCTCCTGCA
    CACATTGCCG CCAGCCATCT CTCCGAGCCT GCAAAGCCAG CGCATTCTCA GAATCAATCA
    AATTTTTCTA GTTATTCTTC AAAGGGAAAG CCTCCTGAAG CTGATGGTGT GGATAGATCA
    TTTGGCGAGA AACGCTATGA ACCCATCCAG GCCACTCCCC CTCCTCCTCC ATTGCCCTCG
    CAGTATGCCC AGCCATCTCA GCCTGTCACC AGCGCGTCTC TCCACATACA TTCTAAGGGA
    GCACATGGTG AAGGTAATTC AGTGTCATTG GATTTTCAGA ATTCCTTAGT GTCCAAACCA
    GACCCACCTC CATCTCAGAA TAAGCCAGCA ACTTTCAGAC CACCAAACCG AGAAGATACT
    GCTCAGGCAG CTTTCTATCC CCAGAAAAGT TTTCCAGATA AAGCCCCAGT TAATGGAACT
    GAACAGACTC AGAAAACAGT CACTCCAGCA TACAATCGAT TCACACCAAA ACCATATACA
    AGTTCTGCCC GACCATTTGA ACGCAAGTTT GAAAGTCCTA AATTCAATCA CAATCTTCTG
    CCAAGTGAAA CTGCACATAA ACCTGACTTG TCTTCAAAAA CTCCCACTTC TCCAAAAACT
    CTTGTGAAAT CGCACAGTTT GGCACAGCCT CCTGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTATCCATG CAGAGAAGCC TAAATATCAA ATAAATAATA TCAGCACAGT GCCTAAAGCT
    ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG GATGAAGATG AAGATGGTCA TACTGTGGTG
    GCCACAGCCC GAGGCATATT TAACAGCAAT GGGGGCGTGC TGAGTTCCAT AGAAACTGGT
    GTTAGTATAA TTATCCCTCA AGGAGCCATT CCCGAAGGAG TTGAGCAGGA AATCTATTTC
    AAGGTCTGCC GGGACAACAG CATCCTTCCA CCTTTAGATA AAGAGAAAGG TGAAACACTG
    CTGAGTCCTT TGGTGATGTG TGGTCCCCAT GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG
    CGCTTACCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT
    TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287954.1
    CDS39..5546
    Translation

    Target ORF information:

    RefSeq Version NM_001301025.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001301025.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    ATGAAGTACC AGAAATACCT GACGGTGCTG CAGATGGCCA TCGGCGTCAC CCCCTCCAAC 
    CGCGGCAGCC TCCTGCCGCT CAAGAGGAAG CTGTGGGTAA CGCCATCCTC TGAAAATCCT
    AATGGTGCTA CTTCTAGTGT CAGCCAAGGA AAACCCTCTT TAAGACGAAT TAAAGGGAGA
    TTACACAGAA GCAAAAGCCT TGATAGCATG GATTTCTGTG AGCTCACTAG CACAGCAATG
    GAGGAAACAG CTATATGGGA ACAACATACA GTGACGCTTC ACAGGGCTCC TGGATTTGGA
    TTTGGAATTG CAATATCTGG TGGACGAGAT AATCCTCATT TTCAGAGTGG GGAAACGTCA
    ATAGTGATTT CAGATGTGCT GAAAGGAGGA CCAGCTGAAG GACAGCTACA GGAAAATGAC
    CGAGTTGCAA TGGTTAACGG AGTTTCAATG GATAATGTTG AACATGCTTT TGCTGTTCAG
    CAACTAAGGA AAAGTGGGAA AAATGCAAAA ATTACAATTA GAAGGAAGAA GAAAGTTCAA
    ATACCAGTAA GTCGTCCTGA TCCTGAACCA GTATCTGATA ATGAAGAAGA TAGTTATGAT
    GAGGAAATAC ATGATCCAAG AAGTGGCCGG AGTGGTGTGG TTAACAGAAG GAGTGAGAAG
    ATTTGGCCGA GGGATAGAAG TGCAAGTAGA GAGAGGAGCT TGTCCCCGCG GTCAGACAGG
    CGGTCAGTGG CTTCCAGCCA GCCTGCTAAA CCTACTAAAG TCACACTGGT GAAATCCCGG
    AAAAATGAAG AATATGGTCT TCGATTGGCA AGCCATATAT TTGTTAAGGA AATTTCACAA
    GATAGTTTGG CAGCAAGAGA TGGCAATATT CAAGAAGGTG ATGTTGTATT GAAGATAAAT
    GGTACTGTGA CAGAAAATAT GTCATTGACA GATGCAAAGA CATTGATAGA AAGGTCTAAA
    GGCAAATTAA AAATGGTAGT TCAAAGAGAT GAACGGGCTA CGCTATTGAA TGTCCCTGAT
    CTTTCTGACA GCATCCACTC TGCTAATGCC TCTGAGAGAG ACGACATTTC AGAAATTCAG
    TCACTGGCAT CAGATCATTC TGGTCGATCA CACGATAGGC CTCCCCGCCG CAGCCGGTCA
    CGATCTCCTG ACCAGCGGTC AGAGCCTTCT GATCATTCCA GGCACTCGCC GCAGCAGCCA
    AGCAATGGCA GTCTCCGGAG TAGAGATGAA GAGAGAATTT CTAAACCTGG GGCTGTCTCA
    ACTCCTGTAA AGCATGCTGA TGATCACACA CCTAAAACAG TGGAAGAAGT TACAGTTGAA
    AGAAATGAGA AACAAACACC TTCTCTTCCA GAACCAAAGC CTGTGTATGC CCAAGTTGGG
    CAACCAGATG TGGATTTACC TGTCAGTCCA TCTGATGGTG TCCTACCTAA TTCAACTCAT
    GAAGATGGGA TTCTTCGGCC CAGCATGAAA TTGGTAAAAT TCAGAAAAGG AGATAGTGTG
    GGTTTGCGGC TGGCTGGTGG AAATGATGTT GGAATATTTG TAGCTGGCGT TCTAGAAGAT
    AGCCCTGCAG CCAAGGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT AAACAACGTA
    GATTTTACAA ATATCATAAG AGAAGAAGCC GTCCTTTTCC TGCTTGACCT CCCTAAAGGA
    GAAGAAGTGA CCATATTGGC TCAGAAGAAG AAGGATGTTT ATCGTCGCAT TGTAGAATCA
    GATGTAGGAG ATTCTTTCTA TATTAGAACC CATTTTGAAT ATGAAAAGGA ATCTCCCTAT
    GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGTGTTGTGG ATACCTTGTA CAATGGAAAA
    CTGGGCTCTT GGCTTGCTAT TCGAATTGGT AAAAATCATA AGGAGGTAGA ACGAGGCATC
    ATCCCTAATA AGAACAGAGC TGAGCAGCTA GCCAGTGTAC AGTATACACT TCCAAAAACA
    GCAGGCGGAG ACCGTGCTGA CTTCTGGAGA TTCAGAGGTC TTCGCAGCTC CAAGAGAAAT
    CTTCGAAAAA GCAGAGAGGA TTTGTCCGCT CAGCCTGTTC AAACAAAGTT TCCAGCTTAT
    GAAAGAGTGG TTCTTCGAGA AGCTGGATTT CTGAGGCCTG TAACCATTTT TGGACCAATA
    GCTGATGTTG CCAGAGAAAA GCTGGCAAGA GAAGAACCAG ATATTTATCA AATTGCAAAG
    AGTGAACCAC GAGACGCTGG AACTGACCAA CGTAGCTCTG GCATTATTCG CCTGCATACA
    ATAAAGCAAA TCATAGATCA AGACAAACAT GCTTTATTAG ATGTAACACC AAATGCAGTT
    GATCGTCTTA ACTATGCCCA GTGGTATCCA ATTGTTGTAT TTCTTAACCC TGATTCTAAG
    CAAGGAGTAA AAACAATGAG AATGAGGTTA TGTCCAGAAT CTCGGAAAAG TGCCAGGAAG
    TTATACGAGC GATCTCATAA ACTTCGTAAA AATAATCACC ATCTTTTTAC AACTACAATT
    AACTTAAATT CAATGAATGA TGGTTGGTAT GGTGCGCTGA AAGAAGCAAT TCAACAACAG
    CAAAACCAGC TGGTATGGGT TTCCGAGGGA AAGGCGGATG GTGCTACAAG TGATGACCTT
    GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCTCCAG GTAGTGAATA CTCAATGTAT
    AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ACACAGAAGG CGGGGCCTAC
    ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTTG GGACTCCACC GGAGTCTGCC
    ATTACACGGT CCTCTGAGCC TGTAAGAGAG GACTCCTCTG GAATGCATCA TGAAAACCAA
    ACATATCCTC CTTACTCACC ACAAGCGCAG CCACAACCAA TTCATAGAAT AGACTCCCCT
    GGATTTAAGC CAGCCTCTCA ACAGAAAGCA GAAGCTTCAT CTCCAGTCCC TTACCTTTCG
    CCTGAAACAA ACCCAGCATC ATCAACCTCT GCTGTTAATC ATAATGTAAA TTTAACTAAT
    GTCAGACTGG AGGAGCCCAC CCCAGCTCCT TCCACCTCTT ACTCACCACA AGCTGATTCT
    TTAAGAACAC CAAGTACTGA GGCAGCTCAC ATAATGCTAA GAGATCAAGA ACCATCATTG
    TCGTCGCATG TAGATCCAAC AAAGGTGTAT AGAAAGGATC CATATCCCGA GGAAATGATG
    AGGCAGAACC ATGTTTTGAA ACAGCCAGCC GTTAGTCACC CAGGGCACAG GCCAGACAAA
    GAGCCTAATC TGACCTATGA ACCCCAACTC CCATACGTAG AGAAACAAGC CAGCAGAGAC
    CTCGAGCAGC CCACATACAG ATACGAGTCC TCAAGCTATA CGGACCAGTT TTCTCGAAAC
    TATGAACATC GTCTGCGATA CGAAGATCGC GTCCCCATGT ATGAAGAACA GTGGTCATAT
    TATGATGACA AACAGCCCTA CCCATCTCGG CCACCTTTTG ATAATCAGCA CTCTCAAGAC
    CTTGACTCCA GACAGCATCC CGAAGAGTCC TCAGAACGAG GGTACTTTCC ACGTTTTGAA
    GAGCCAGCCC CTCTGTCTTA CGACAGCAGA CCACGTTACG AACAGGCACC TAGAGCATCC
    GCCCTGCGGC ACGAAGAGCA GCCAGCTCCT GGGTATGACA CACATGGTAG ACTCAGACCG
    GAAGCCCAGC CCCACCCTTC AGCAGGGCCC AAGCCTGCAG AGTCCAAGCA GTATTTTGAG
    CAATATTCAC GCAGTTACGA GCAAGTACCA CCCCAAGGAT TTACCTCTAG AGCAGGTCAT
    TTTGAGCCTC TCCATGGTGC TGCAGCTGTC CCTCCGCTGA TACCTTCATC TCAGCATAAG
    CCAGAAGCTC TGCCTTCAAA CACCAAACCA CTGCCTCCAC CCCCAACTCA AACCGAAGAA
    GAGGAAGATC CAGCAATGAA GCCACAGTCT GTACTCACCA GAGTTAAGAT GTTTGAAAAC
    AAAAGATCTG CATCCTTAGA GACCAAGAAG GATGTAAATG ACACTGGCAG TTTTAAGCCT
    CCAGAAGTAG CATCTAAACC TTCAGGTGCT CCCATCATTG GTCCCAAACC CACTTCTCAG
    AATCAATTCA GTGAACATGA CAAAACTCTG TACAGGATCC CAGAACCTCA AAAACCTCAA
    CTGAAGCCAC CTGAAGATAT TGTTCGGTCC AATCATTATG ACCCTGAAGA AGATGAAGAA
    TATTATCGAA AACAGCTGTC ATACTTTGAC CGAAGAAGTT TTGAGAATAA GCCTCCTGCA
    CACATTGCCG CCAGCCATCT CTCCGAGCCT GCAAAGCCAG CGCATTCTCA GAATCAATCA
    AATTTTTCTA GTTATTCTTC AAAGGGAAAG CCTCCTGAAG CTGATGGTGT GGATAGATCA
    TTTGGCGAGA AACGCTATGA ACCCATCCAG GCCACTCCCC CTCCTCCTCC ATTGCCCTCG
    CAGTATGCCC AGCCATCTCA GCCTGTCACC AGCGCGTCTC TCCACATACA TTCTAAGGGA
    GCACATGGTG AAGGTAATTC AGTGTCATTG GATTTTCAGA ATTCCTTAGT GTCCAAACCA
    GACCCACCTC CATCTCAGAA TAAGCCAGCA ACTTTCAGAC CACCAAACCG AGAAGATACT
    GCTCAGGCAG CTTTCTATCC CCAGAAAAGT TTTCCAGATA AAGCCCCAGT TAATGGAACT
    GAACAGACTC AGAAAACAGT CACTCCAGCA TACAATCGAT TCACACCAAA ACCATATACA
    AGTTCTGCCC GACCATTTGA ACGCAAGTTT GAAAGTCCTA AATTCAATCA CAATCTTCTG
    CCAAGTGAAA CTGCACATAA ACCTGACTTG TCTTCAAAAA CTCCCACTTC TCCAAAAACT
    CTTGTGAAAT CGCACAGTTT GGCACAGCCT CCTGAGTTTG ACAGTGGAGT TGAAACTTTC
    TCTATCCATG CAGAGAAGCC TAAATATCAA ATAAATAATA TCAGCACAGT GCCTAAAGCT
    ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG GATGAAGATG AAGATGGTCA TACTGTGGTG
    GCCACAGCCC GAGGCATATT TAACAGCAAT GGGGGCGTGC TGAGTTCCAT AGAAACTGGT
    GTTAGTATAA TTATCCCTCA AGGAGCCATT CCCGAAGGAG TTGAGCAGGA AATCTATTTC
    AAGGTCTGCC GGGACAACAG CATCCTTCCA CCTTTAGATA AAGAGAAAGG TGAAACACTG
    CTGAGTCCTT TGGTGATGTG TGGTCCCCAT GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG
    CGCTTACCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC
    GACTCCTCGT CGGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT
    TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48522
    Clone ID Related Accession (Same CDS sequence) NM_001330239.1 , NM_001330239.2 , NM_001330239.3
    Accession Version NM_001330239.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-23
    Organism Homo sapiens(Human)
    Product tight junction protein ZO-1 isoform e
    Comment VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC022613.13. On Aug 20, 2016 this sequence version replaced XM_005254618.3. Transcript Variant: This variant (5) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (e) is longer than isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.266936.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317168.1
    CDS476..5782
    Translation

    Target ORF information:

    RefSeq Version NM_001330239.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330239.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48522
    Clone ID Related Accession (Same CDS sequence) NM_001330239.1 , NM_001330239.2 , NM_001330239.3
    Accession Version NM_001330239.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022613.13, AB370197.1, CV571135.1, BE819398.1, DA261260.1 and AF035298.1. On Sep 1, 2017 this sequence version replaced NM_001330239.1. Transcript Variant: This variant (5) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (e) is longer than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.266936.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317168.1
    CDS476..5782
    Translation

    Target ORF information:

    RefSeq Version NM_001330239.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330239.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48522
    Clone ID Related Accession (Same CDS sequence) NM_001330239.1 , NM_001330239.2 , NM_001330239.3
    Accession Version NM_001330239.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5307bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform e
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022613.13, AB370197.1, CV571135.1, BE819398.1, DA261260.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_001330239.2. Transcript Variant: This variant (5) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (e) is longer than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.266936.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001317168.1
    CDS441..5747
    Translation

    Target ORF information:

    RefSeq Version NM_001330239.3
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001330239.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
    CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT
    GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG
    AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA
    GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA
    AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT
    CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA
    AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT
    GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG
    CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT
    CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT
    GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG
    TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT
    CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT
    GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT
    GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA
    GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT
    AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT
    GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT
    TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT
    GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC
    AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA
    AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC
    TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA
    GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT
    CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT
    ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA
    GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT
    CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT
    GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC
    TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT
    TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA
    GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG
    CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA
    ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA
    GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG
    TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA
    ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA
    CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT
    GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT
    TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG
    TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT
    TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA
    ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT
    GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA
    CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA
    CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA
    TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC
    CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG
    GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA
    AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA
    CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA
    CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA
    TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC
    GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC
    CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC
    GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC
    GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG
    CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA
    GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG
    CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT
    GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC
    ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG
    CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG
    ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT
    TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC
    AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT
    GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA
    TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC
    TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA
    AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA
    CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG
    CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA
    GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT
    AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC
    CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC
    ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA
    CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA
    CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG
    GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT
    AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT
    GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT
    AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA
    GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC
    ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT
    GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC
    ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48525
    Clone ID Related Accession (Same CDS sequence) NM_001301026.2 , NM_001301026.1
    Accession Version NM_001301026.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640879.1, AC022613.13, AB370197.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_001301026.1. Transcript Variant: This variant (4) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, and it lacks an alternate in-frame exon in the central coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX640879.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287955.1
    CDS174..5252
    Translation

    Target ORF information:

    RefSeq Version NM_001301026.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001301026.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu48525
    Clone ID Related Accession (Same CDS sequence) NM_001301026.2 , NM_001301026.1
    Accession Version NM_001301026.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5079bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-12
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640879.1, AC022613.13, AB370197.1 and AF035298.1. On or before Jul 18, 2014 this sequence version replaced XM_006725559.1, XM_006720661.1. Transcript Variant: This variant (4) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, and it lacks an alternate in-frame exon in the central coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX640879.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287955.1
    CDS243..5321
    Translation

    Target ORF information:

    RefSeq Version NM_001301026.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001301026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
    GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT
    GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT
    TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA
    ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG
    AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA
    AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA
    CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG
    AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG
    GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA
    GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG
    GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG
    ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA
    AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC
    AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA
    TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT
    GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC
    AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA
    AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG
    AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT
    GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG
    ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG
    CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA
    GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA
    AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG
    ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA
    GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT
    TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT
    TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT
    AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA
    GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA
    AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG
    GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT
    GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA
    CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA
    ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT
    AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA
    AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG
    CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT
    TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG
    CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT
    GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC
    AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA
    GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG
    TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT
    CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG
    CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC
    CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT
    CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG
    CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT
    CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC
    AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC
    AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC
    CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG
    CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG
    CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA
    CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT
    CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT
    CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT
    CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT
    GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA
    GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC
    AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA
    CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA
    AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC
    GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT
    AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG
    AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC
    CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT
    GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT
    CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA
    GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT
    CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC
    CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA
    ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA
    TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT
    GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG
    AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC
    CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA
    ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC
    CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT
    TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA
    CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG
    TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT
    GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102941
    Clone ID Related Accession (Same CDS sequence) NM_001301025.3 , NM_001301025.2
    Accession Version NM_001301025.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087455.7, AC022613.13 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_001301025.2. Transcript Variant: This variant (3) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, and it uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is longer than isoform a. The exon combination of this variant is inferred based on partial transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.29109.1, SRR1803611.232016.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287954.2
    CDS211..5796
    Translation

    Target ORF information:

    RefSeq Version NM_001301025.3
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001301025.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu102941
    Clone ID Related Accession (Same CDS sequence) NM_001301025.3 , NM_001301025.2
    Accession Version NM_001301025.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5586bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-12
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087455.7, AC022613.13 and AF035298.1. On Sep 1, 2017 this sequence version replaced NM_001301025.1. Transcript Variant: This variant (3) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, and it uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is longer than isoform a. The exon combination of this variant is inferred based on partial transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.29109.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001287954.2
    CDS86..5671
    Translation

    Target ORF information:

    RefSeq Version NM_001301025.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001301025.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85630
    Clone ID Related Accession (Same CDS sequence) NM_001355012.1 , XM_005254617.3 , NM_001355012.2
    Accession Version NM_001355012.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-12
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087455.7, CN372462.1, CN372473.1, CD653673.1, BC111712.1, BQ434619.1, AL707462.1, BE082525.1, HY197781.1, AA101028.1, BX504167.1, DB089107.1, CV571135.1, BQ575449.1, AC022613.13, BE819398.1, BI914113.1, BM459712.1, BX640879.1 and AF035298.1. On Sep 1, 2017 this sequence version replaced XM_005254617.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.27168.1, SRR1803617.114641.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341941.1
    CDS86..5611
    Translation

    Target ORF information:

    RefSeq Version NM_001355012.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355012.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85630
    Clone ID Related Accession (Same CDS sequence) NM_001355012.1 , XM_005254617.3 , NM_001355012.2
    Accession Version NM_001355012.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-15
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform f
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087455.7, CN372462.1, CN372473.1, CD653673.1, BC111712.1, BQ434619.1, AL707462.1, BE082525.1, HY197781.1, AA101028.1, BX504167.1, DB089107.1, CV571135.1, BQ575449.1, AC022613.13, BE819398.1, BI914113.1, BM459712.1, BX640879.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_001355012.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.27168.1, SRR1803617.114641.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001341941.1
    CDS211..5736
    Translation

    Target ORF information:

    RefSeq Version NM_001355012.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001355012.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85626
    Clone ID Related Accession (Same CDS sequence) XM_017022521.1
    Accession Version XM_017022521.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5637bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT
    AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG
    GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA
    TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT
    TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT
    AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA
    CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT
    CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT
    ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT
    GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT
    GTGGAGCTGC GCTTACCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCCGGA
    GATCCAAATT ATCTCGTTGG AGCAAACTGT GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878010.1
    CDS81..5717
    Translation

    Target ORF information:

    RefSeq Version XM_017022521.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022521.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT
    AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG
    GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA
    TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT
    TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT
    AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA
    CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT
    CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT
    ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT
    GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT
    GTGGAGCTGC GCTTACCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCCGGA
    GATCCAAATT ATCTCGTTGG AGCAAACTGT GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85627
    Clone ID Related Accession (Same CDS sequence) XM_017022522.1
    Accession Version XM_017022522.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5598bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT
    AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG
    GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA
    TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT
    TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT
    AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA
    CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT
    CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT
    ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT
    GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT
    GTGGAGCTGC GCTTACCACA CTGTGCGTCC ATGACTCCTG ACGGTGATCC TAAAACCTGG
    CAAAACAAGT GTCTTCCCGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC
    CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878011.1
    CDS81..5678
    Translation

    Target ORF information:

    RefSeq Version XM_017022522.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022522.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT
    AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG
    GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA
    TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT
    TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT
    AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA
    CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT
    CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT
    ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT
    GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT
    GTGGAGCTGC GCTTACCACA CTGTGCGTCC ATGACTCCTG ACGGTGATCC TAAAACCTGG
    CAAAACAAGT GTCTTCCCGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC
    CTTATTGACC ACTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85628
    Clone ID Related Accession (Same CDS sequence) XM_017022523.1
    Accession Version XM_017022523.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5577bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT
    AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG
    GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA
    TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT
    TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT
    AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA
    CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT
    CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT
    ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT
    GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT
    GTGGAGCTGC GCTTACCACA CTGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCCGGA
    GATCCAAATT ATCTCGTTGG AGCAAACTGT GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878012.1
    CDS81..5657
    Translation

    Target ORF information:

    RefSeq Version XM_017022523.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022523.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT
    AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG
    GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA
    TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT
    TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT
    AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA
    CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT
    CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT
    ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT
    GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT
    GTGGAGCTGC GCTTACCACA CTGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCCGGA
    GATCCAAATT ATCTCGTTGG AGCAAACTGT GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85629
    Clone ID Related Accession (Same CDS sequence) XM_011521972.2
    Accession Version XM_011521972.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5547bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011520274.2
    CDS81..5627
    Translation

    Target ORF information:

    RefSeq Version XM_011521972.2
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011521972.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT
    AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT
    GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85630
    Clone ID Related Accession (Same CDS sequence) NM_001355012.1 , XM_005254617.3 , NM_001355012.2
    Accession Version XM_005254617.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5526bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(Human)
    Product tight junction protein ZO-1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254617.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254674.2
    CDS81..5606
    Translation

    Target ORF information:

    RefSeq Version XM_005254617.3
    Organism Homo sapiens(Human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254617.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT
    CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT
    AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC
    TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA
    GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85631
    Clone ID Related Accession (Same CDS sequence) XM_017022524.1
    Accession Version XM_017022524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5397bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT
    AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG
    GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA
    TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT
    TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT
    AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA
    CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT
    CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT
    ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT
    GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT
    GTGGAGCTGC GCTTACCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCCGGA
    GATCCAAATT ATCTCGTTGG AGCAAACTGT GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016878013.1
    CDS81..5477
    Translation

    Target ORF information:

    RefSeq Version XM_017022524.1
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017022524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT
    AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG
    GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA
    TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT
    TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG
    TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA
    GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT
    AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA
    CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC
    AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT
    CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT
    GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG
    CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT
    ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA
    GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA
    ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT
    GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT
    GTGGAGCTGC GCTTACCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA
    AAATCATCCG ACTCCTCGTC GGGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCCGGA
    GATCCAAATT ATCTCGTTGG AGCAAACTGT GTTTCTGTCC TTATTGACCA CTTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85635
    Clone ID Related Accession (Same CDS sequence) XM_005254619.3
    Accession Version XM_005254619.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5346bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254619.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254676.2
    CDS81..5426
    Translation

    Target ORF information:

    RefSeq Version XM_005254619.3
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254619.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT
    TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu85636
    Clone ID Related Accession (Same CDS sequence) XM_005254620.3
    Accession Version XM_005254620.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5286bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product tight junction protein ZO-1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254620.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005254677.2
    CDS81..5366
    Translation

    Target ORF information:

    RefSeq Version XM_005254620.3
    Organism Homo sapiens(human)
    Definition Homo sapiens tight junction protein 1 (TJP1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005254620.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
    GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC
    GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG
    CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA
    AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG
    CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC
    AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT
    CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA
    CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA
    CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA
    AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT
    GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT
    AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG
    TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC
    ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT
    GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT
    GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA
    TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG
    CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC
    GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT
    CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG
    CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT
    AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG
    GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT
    GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC
    CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC
    AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA
    GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT
    CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG
    CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT
    CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT
    GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT
    ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG
    GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG
    TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT
    CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA
    ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA
    ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT
    ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC
    ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT
    GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT
    CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT
    CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT
    CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA
    GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT
    GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT
    AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC
    ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG
    ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA
    ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT
    CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA
    TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA
    GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG
    AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG
    GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT
    GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT
    AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG
    TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA
    CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA
    CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG
    TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT
    ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA
    CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC
    CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA
    GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC
    ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT
    CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA
    GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC
    CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT
    GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG
    CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT
    GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT
    CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC
    CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT
    TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA
    CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA
    GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC
    ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA
    TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT
    CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC
    AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC
    AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA
    GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG
    AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT
    GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA
    GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC
    CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT
    CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC
    TTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.