TJP1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_017022529.1	XP_016878018.1	tight junction protein ZO-1 isoform X14
XM_017022530.1	XP_016878019.1	tight junction protein ZO-1 isoform X15
NM_001330239.1	NP_001317168.1	tight junction protein ZO-1 isoform e
NM_001355014.1	NP_001341943.1	tight junction protein ZO-1 isoform h
NM_175610.3	NP_783297.2	tight junction protein ZO-1 isoform b
XM_017022521.1	XP_016878010.1	tight junction protein ZO-1 isoform X1
NM_001301026.2	NP_001287955.1	tight junction protein ZO-1 isoform d
NM_001301025.3	NP_001287954.2	tight junction protein ZO-1 isoform c
XM_017022523.1	XP_016878012.1	tight junction protein ZO-1 isoform X3
XM_017022525.1	XP_016878014.1	tight junction protein ZO-1 isoform X6
XM_005254620.3	XP_005254677.2	tight junction protein ZO-1 isoform X10
NM_001301025.2	NP_001287954.2	tight junction protein ZO-1 isoform c
NM_001301025.1	NP_001287954.1	tight junction protein ZO-1 isoform c
XM_017022528.1	XP_016878017.1	tight junction protein ZO-1 isoform X13
NM_001301026.1	NP_001287955.1	tight junction protein ZO-1 isoform d
XM_017022522.1	XP_016878011.1	tight junction protein ZO-1 isoform X2
XM_017022527.1	XP_016878016.1	tight junction protein ZO-1 isoform X8
XM_005254619.3	XP_005254676.2	tight junction protein ZO-1 isoform X9
NM_175610.4	NP_783297.2	tight junction protein ZO-1 isoform b
NM_003257.4	NP_003248.3	tight junction protein ZO-1 isoform a
NM_001330239.2	NP_001317168.1	tight junction protein ZO-1 isoform e
XM_017022524.1	XP_016878013.1	tight junction protein ZO-1 isoform X5
XM_017022531.1	XP_016878020.1	tight junction protein ZO-1 isoform X11
NM_001330239.3	NP_001317168.1	tight junction protein ZO-1 isoform e
NM_001355014.2	NP_001341943.1	tight junction protein ZO-1 isoform h
NM_001355015.1	NP_001341944.1	tight junction protein ZO-1 isoform i
NM_001355012.1	NP_001341941.1	tight junction protein ZO-1 isoform f
XM_005254617.3	XP_005254674.2	tight junction protein ZO-1 isoform X5
XM_011521972.2	XP_011520274.2	tight junction protein ZO-1 isoform X4
XM_017022526.1	XP_016878015.1	tight junction protein ZO-1 isoform X7
NM_003257.5	NP_003248.3	tight junction protein ZO-1 isoform a
NM_001355013.1	NP_001341942.1	tight junction protein ZO-1 isoform g
NM_001355012.2	NP_001341941.1	tight junction protein ZO-1 isoform f
NM_001355015.2	NP_001341944.1	tight junction protein ZO-1 isoform i

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25467	NM_003257.5 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu25544	NM_175610.4 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu85640	XM_017022531.1 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu85637	NM_001355013.1 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85638	NM_001355014.2 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85639	NM_001355015.2 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant 9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85632	XM_017022525.1 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85633	XM_017022526.1 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85634	XM_017022527.1 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85637	XM_017022528.1 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85638	XM_017022529.1 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu85639	XM_017022530.1 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu48519	NM_001301025.1 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu48522	NM_001330239.3 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu48525	NM_001301026.2 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu102941	NM_001301025.3 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85630	NM_001355012.2 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85626	XM_017022521.1 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85627	XM_017022522.1 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85628	XM_017022523.1 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85629	XM_011521972.2 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85630	XM_005254617.3 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85631	XM_017022524.1 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85635	XM_005254619.3 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85636	XM_005254620.3 Latest version!	Homo sapiens tight junction protein 1 (TJP1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
View Old Accession Versions >>
OHu102941	NM_001301025.2	Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu48525	NM_001301026.1	Homo sapiens tight junction protein 1 (TJP1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu25544	NM_175610.3	Homo sapiens tight junction protein 1 (TJP1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu48522	NM_001330239.1	Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu48522	NM_001330239.2	Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu85638	NM_001355014.1	Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu25467	NM_003257.4	Homo sapiens tight junction protein 1 (TJP1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$679.50-$951.30 $1359.00
OHu85630	NM_001355012.1	Homo sapiens tight junction protein 1 (TJP1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$951.30 $1359.00
OHu85639	NM_001355015.1	Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

TJP1 ( NM_003257.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003248.3 Homo sapiens tight junction protein 1 (TJP1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25467
Clone ID Related Accession (Same CDS sequence)	NM_003257.4 , NM_003257.5
Accession Version	NM_003257.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AC022613.13, BX640879.1 and AF035298.1. On Jul 18, 2014 this sequence version replaced NM_003257.3. Transcript Variant: This variant (1, also known as ZO-1alpha+) encodes isoform a, which is found in most epithelial cell junctions. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC111712.1, AK304758.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003248.3
CDS	476..5722
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_003257.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003257.4

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_003257.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_003248.3 Homo sapiens tight junction protein 1 (TJP1), transcript variant 1, mRNA.

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CloneID	OHu25467
Clone ID Related Accession (Same CDS sequence)	NM_003257.4 , NM_003257.5
Accession Version	NM_003257.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5247bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AC022613.13, BX640879.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_003257.4. Transcript Variant: This variant (1, also known as ZO-1alpha+) encodes isoform a, which is found in most epithelial cell junctions. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC111712.1, AK304758.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003248.3
CDS	441..5687
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_003257.5
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003257.5

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_175610.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_783297.2 Homo sapiens tight junction protein 1 (TJP1), transcript variant 2, mRNA.

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CloneID	OHu25544
Clone ID Related Accession (Same CDS sequence)	NM_175610.3 , NM_175610.4
Accession Version	NM_175610.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-12
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AK302971.1, AC022613.13, BX640879.1 and AF035298.1. On Jul 18, 2014 this sequence version replaced NM_175610.2. Transcript Variant: This variant (2, also known as ZO-1alpha-) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Isoform b is found in both endothelial cells and the highly specialized epithelial junctions of renal glomeruli and sertoli cells of the seminiferous tubules. The exon combination of this variant is inferred based on partial transcript alignments and data in PMID:1590354. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.267789.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_783297.2
CDS	476..5482
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_175610.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175610.3

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_175610.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_783297.2 Homo sapiens tight junction protein 1 (TJP1), transcript variant 2, mRNA.

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CloneID	OHu25544
Clone ID Related Accession (Same CDS sequence)	NM_175610.3 , NM_175610.4
Accession Version	NM_175610.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5007bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AK302971.1, AC022613.13, BX640879.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_175610.3. Transcript Variant: This variant (2, also known as ZO-1alpha-) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in an isoform (b) that is shorter than isoform a. Isoform b is found in both endothelial cells and the highly specialized epithelial junctions of renal glomeruli and sertoli cells of the seminiferous tubules. The exon combination of this variant is inferred based on partial transcript alignments and data in PMID:1590354. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.267789.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_783297.2
CDS	441..5447
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_175610.4
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175610.4

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017022531.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878020.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu85640
Clone ID Related Accession (Same CDS sequence)	XM_017022531.1
Accession Version	XM_017022531.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4359bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACACACA GTCTCCGGAG TAGAGATGAA GAGAGAATTT CTAAACCTGG GGCTGTCTCA 
ACTCCTGTAA AGCATGCTGA TGATCACACA CCTAAAACAG TGGAAGAAGT TACAGTTGAA 
AGAAATGAGA AACAAACACC TTCTCTTCCA GAACCAAAGC CTGTGTATGC CCAAGTTGGG 
CAACCAGATG TGGATTTACC TGTCAGTCCA TCTGATGGTG TCCTACCTAA TTCAACTCAT 
GAAGATGGGA TTCTTCGGCC CAGCATGAAA TTGGTAAAAT TCAGAAAAGG AGATAGTGTG 
GGTTTGCGGC TGGCTGGTGG AAATGATGTT GGAATATTTG TAGCTGGCGT TCTAGAAGAT 
AGCCCTGCAG CCAAGGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT AAACAACGTA 
GATTTTACAA ATATCATAAG AGAAGAAGCC GTCCTTTTCC TGCTTGACCT CCCTAAAGGA 
GAAGAAGTGA CCATATTGGC TCAGAAGAAG AAGGATGTTT ATCGTCGCAT TGTAGAATCA 
GATGTAGGAG ATTCTTTCTA TATTAGAACC CATTTTGAAT ATGAAAAGGA ATCTCCCTAT 
GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGTGTTGTGG ATACCTTGTA CAATGGAAAA 
CTGGGCTCTT GGCTTGCTAT TCGAATTGGT AAAAATCATA AGGAGGTAGA ACGAGGCATC 
ATCCCTAATA AGAACAGAGC TGAGCAGCTA GCCAGTGTAC AGTATACACT TCCAAAAACA 
GCAGGCGGAG ACCGTGCTGA CTTCTGGAGA TTCAGAGGTC TTCGCAGCTC CAAGAGAAAT 
CTTCGAAAAA GCAGAGAGGA TTTGTCCGCT CAGCCTGTTC AAACAAAGTT TCCAGCTTAT 
GAAAGAGTGG TTCTTCGAGA AGCTGGATTT CTGAGGCCTG TAACCATTTT TGGACCAATA 
GCTGATGTTG CCAGAGAAAA GCTGGCAAGA GAAGAACCAG ATATTTATCA AATTGCAAAG 
AGTGAACCAC GAGACGCTGG AACTGACCAA CGTAGCTCTG GCATTATTCG CCTGCATACA 
ATAAAGCAAA TCATAGATCA AGACAAACAT GCTTTATTAG ATGTAACACC AAATGCAGTT 
GATCGTCTTA ACTATGCCCA GTGGTATCCA ATTGTTGTAT TTCTTAACCC TGATTCTAAG 
CAAGGAGTAA AAACAATGAG AATGAGGTTA TGTCCAGAAT CTCGGAAAAG TGCCAGGAAG 
TTATACGAGC GATCTCATAA ACTTCGTAAA AATAATCACC ATCTTTTTAC AACTACAATT 
AACTTAAATT CAATGAATGA TGGTTGGTAT GGTGCGCTGA AAGAAGCAAT TCAACAACAG 
CAAAACCAGC TGGTATGGGT TTCCGAGGGA AAGGCGGATG GTGCTACAAG TGATGACCTT 
GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCTCCAG GTAGTGAATA CTCAATGTAT 
AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ACACAGAAGG CGGGGCCTAC 
ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTTG GGACTCCACC GGAGTCTGCC 
ATTACACGGT CCTCTGAGCC TGTAAGAGAG GACTCCTCTG GAATGCATCA TGAAAACCAA 
ACATATCCTC CTTACTCACC ACAAGCGCAG CCACAACCAA TTCATAGAAT AGACTCCCCT 
GGATTTAAGC CAGCCTCTCA ACAGAAAGCA GAAGCTTCAT CTCCAGTCCC TTACCTTTCG 
CCTGAAACAA ACCCAGCATC ATCAACCTCT GCTGTTAATC ATAATGTAAA TTTAACTAAT 
GTCAGACTGG AGGAGCCCAC CCCAGCTCCT TCCACCTCTT ACTCACCACA AGCTGATTCT 
TTAAGAACAC CAAGTACTGA GGCAGCTCAC ATAATGCTAA GAGATCAAGA ACCATCATTG 
TCGTCGCATG TAGATCCAAC AAAGGTGTAT AGAAAGGATC CATATCCCGA GGAAATGATG 
AGGCAGAACC ATGTTTTGAA ACAGCCAGCC GTTAGTCACC CAGGGCACAG GCCAGACAAA 
GAGCCTAATC TGACCTATGA ACCCCAACTC CCATACGTAG AGAAACAAGC CAGCAGAGAC 
CTCGAGCAGC CCACATACAG ATACGAGTCC TCAAGCTATA CGGACCAGTT TTCTCGAAAC 
TATGAACATC GTCTGCGATA CGAAGATCGC GTCCCCATGT ATGAAGAACA GTGGTCATAT 
TATGATGACA AACAGCCCTA CCCATCTCGG CCACCTTTTG ATAATCAGCA CTCTCAAGAC 
CTTGACTCCA GACAGCATCC CGAAGAGTCC TCAGAACGAG GGTACTTTCC ACGTTTTGAA 
GAGCCAGCCC CTCTGTCTTA CGACAGCAGA CCACGTTACG AACAGGCACC TAGAGCATCC 
GCCCTGCGGC ACGAAGAGCA GCCAGCTCCT GGGTATGACA CACATGGTAG ACTCAGACCG 
GAAGCCCAGC CCCACCCTTC AGCAGGGCCC AAGCCTGCAG AGTCCAAGCA GTATTTTGAG 
CAATATTCAC GCAGTTACGA GCAAGTACCA CCCCAAGGAT TTACCTCTAG AGCAGGTCAT 
TTTGAGCCTC TCCATGGTGC TGCAGCTGTC CCTCCGCTGA TACCTTCATC TCAGCATAAG 
CCAGAAGCTC TGCCTTCAAA CACCAAACCA CTGCCTCCAC CCCCAACTCA AACCGAAGAA 
GAGGAAGATC CAGCAATGAA GCCACAGTCT GTACTCACCA GAGTTAAGAT GTTTGAAAAC 
AAAAGATCTG CATCCTTAGA GACCAAGAAG GATGTAAATG ACACTGGCAG TTTTAAGCCT 
CCAGAAGTAG CATCTAAACC TTCAGGTGCT CCCATCATTG GTCCCAAACC CACTTCTCAG 
AATCAATTCA GTGAACATGA CAAAACTCTG TACAGGATCC CAGAACCTCA AAAACCTCAA 
CTGAAGCCAC CTGAAGATAT TGTTCGGTCC AATCATTATG ACCCTGAAGA AGATGAAGAA 
TATTATCGAA AACAGCTGTC ATACTTTGAC CGAAGAAGTT TTGAGAATAA GCCTCCTGCA 
CACATTGCCG CCAGCCATCT CTCCGAGCCT GCAAAGCCAG CGCATTCTCA GAATCAATCA 
AATTTTTCTA GTTATTCTTC AAAATTTCTT GGCTCTTACA CTAGCTATGA CTACTTGAAA 
AGGAACGTCA AGTGGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878020.1
CDS	1189..5547
Translation	MTHSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKFLGSYTSYDYLKRNVKWGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017022531.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022531.1

ORF Insert Sequence:

ATGACACACA GTCTCCGGAG TAGAGATGAA GAGAGAATTT CTAAACCTGG GGCTGTCTCA 
ACTCCTGTAA AGCATGCTGA TGATCACACA CCTAAAACAG TGGAAGAAGT TACAGTTGAA 
AGAAATGAGA AACAAACACC TTCTCTTCCA GAACCAAAGC CTGTGTATGC CCAAGTTGGG 
CAACCAGATG TGGATTTACC TGTCAGTCCA TCTGATGGTG TCCTACCTAA TTCAACTCAT 
GAAGATGGGA TTCTTCGGCC CAGCATGAAA TTGGTAAAAT TCAGAAAAGG AGATAGTGTG 
GGTTTGCGGC TGGCTGGTGG AAATGATGTT GGAATATTTG TAGCTGGCGT TCTAGAAGAT 
AGCCCTGCAG CCAAGGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT AAACAACGTA 
GATTTTACAA ATATCATAAG AGAAGAAGCC GTCCTTTTCC TGCTTGACCT CCCTAAAGGA 
GAAGAAGTGA CCATATTGGC TCAGAAGAAG AAGGATGTTT ATCGTCGCAT TGTAGAATCA 
GATGTAGGAG ATTCTTTCTA TATTAGAACC CATTTTGAAT ATGAAAAGGA ATCTCCCTAT 
GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGTGTTGTGG ATACCTTGTA CAATGGAAAA 
CTGGGCTCTT GGCTTGCTAT TCGAATTGGT AAAAATCATA AGGAGGTAGA ACGAGGCATC 
ATCCCTAATA AGAACAGAGC TGAGCAGCTA GCCAGTGTAC AGTATACACT TCCAAAAACA 
GCAGGCGGAG ACCGTGCTGA CTTCTGGAGA TTCAGAGGTC TTCGCAGCTC CAAGAGAAAT 
CTTCGAAAAA GCAGAGAGGA TTTGTCCGCT CAGCCTGTTC AAACAAAGTT TCCAGCTTAT 
GAAAGAGTGG TTCTTCGAGA AGCTGGATTT CTGAGGCCTG TAACCATTTT TGGACCAATA 
GCTGATGTTG CCAGAGAAAA GCTGGCAAGA GAAGAACCAG ATATTTATCA AATTGCAAAG 
AGTGAACCAC GAGACGCTGG AACTGACCAA CGTAGCTCTG GCATTATTCG CCTGCATACA 
ATAAAGCAAA TCATAGATCA AGACAAACAT GCTTTATTAG ATGTAACACC AAATGCAGTT 
GATCGTCTTA ACTATGCCCA GTGGTATCCA ATTGTTGTAT TTCTTAACCC TGATTCTAAG 
CAAGGAGTAA AAACAATGAG AATGAGGTTA TGTCCAGAAT CTCGGAAAAG TGCCAGGAAG 
TTATACGAGC GATCTCATAA ACTTCGTAAA AATAATCACC ATCTTTTTAC AACTACAATT 
AACTTAAATT CAATGAATGA TGGTTGGTAT GGTGCGCTGA AAGAAGCAAT TCAACAACAG 
CAAAACCAGC TGGTATGGGT TTCCGAGGGA AAGGCGGATG GTGCTACAAG TGATGACCTT 
GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCTCCAG GTAGTGAATA CTCAATGTAT 
AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ACACAGAAGG CGGGGCCTAC 
ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTTG GGACTCCACC GGAGTCTGCC 
ATTACACGGT CCTCTGAGCC TGTAAGAGAG GACTCCTCTG GAATGCATCA TGAAAACCAA 
ACATATCCTC CTTACTCACC ACAAGCGCAG CCACAACCAA TTCATAGAAT AGACTCCCCT 
GGATTTAAGC CAGCCTCTCA ACAGAAAGCA GAAGCTTCAT CTCCAGTCCC TTACCTTTCG 
CCTGAAACAA ACCCAGCATC ATCAACCTCT GCTGTTAATC ATAATGTAAA TTTAACTAAT 
GTCAGACTGG AGGAGCCCAC CCCAGCTCCT TCCACCTCTT ACTCACCACA AGCTGATTCT 
TTAAGAACAC CAAGTACTGA GGCAGCTCAC ATAATGCTAA GAGATCAAGA ACCATCATTG 
TCGTCGCATG TAGATCCAAC AAAGGTGTAT AGAAAGGATC CATATCCCGA GGAAATGATG 
AGGCAGAACC ATGTTTTGAA ACAGCCAGCC GTTAGTCACC CAGGGCACAG GCCAGACAAA 
GAGCCTAATC TGACCTATGA ACCCCAACTC CCATACGTAG AGAAACAAGC CAGCAGAGAC 
CTCGAGCAGC CCACATACAG ATACGAGTCC TCAAGCTATA CGGACCAGTT TTCTCGAAAC 
TATGAACATC GTCTGCGATA CGAAGATCGC GTCCCCATGT ATGAAGAACA GTGGTCATAT 
TATGATGACA AACAGCCCTA CCCATCTCGG CCACCTTTTG ATAATCAGCA CTCTCAAGAC 
CTTGACTCCA GACAGCATCC CGAAGAGTCC TCAGAACGAG GGTACTTTCC ACGTTTTGAA 
GAGCCAGCCC CTCTGTCTTA CGACAGCAGA CCACGTTACG AACAGGCACC TAGAGCATCC 
GCCCTGCGGC ACGAAGAGCA GCCAGCTCCT GGGTATGACA CACATGGTAG ACTCAGACCG 
GAAGCCCAGC CCCACCCTTC AGCAGGGCCC AAGCCTGCAG AGTCCAAGCA GTATTTTGAG 
CAATATTCAC GCAGTTACGA GCAAGTACCA CCCCAAGGAT TTACCTCTAG AGCAGGTCAT 
TTTGAGCCTC TCCATGGTGC TGCAGCTGTC CCTCCGCTGA TACCTTCATC TCAGCATAAG 
CCAGAAGCTC TGCCTTCAAA CACCAAACCA CTGCCTCCAC CCCCAACTCA AACCGAAGAA 
GAGGAAGATC CAGCAATGAA GCCACAGTCT GTACTCACCA GAGTTAAGAT GTTTGAAAAC 
AAAAGATCTG CATCCTTAGA GACCAAGAAG GATGTAAATG ACACTGGCAG TTTTAAGCCT 
CCAGAAGTAG CATCTAAACC TTCAGGTGCT CCCATCATTG GTCCCAAACC CACTTCTCAG 
AATCAATTCA GTGAACATGA CAAAACTCTG TACAGGATCC CAGAACCTCA AAAACCTCAA 
CTGAAGCCAC CTGAAGATAT TGTTCGGTCC AATCATTATG ACCCTGAAGA AGATGAAGAA 
TATTATCGAA AACAGCTGTC ATACTTTGAC CGAAGAAGTT TTGAGAATAA GCCTCCTGCA 
CACATTGCCG CCAGCCATCT CTCCGAGCCT GCAAAGCCAG CGCATTCTCA GAATCAATCA 
AATTTTTCTA GTTATTCTTC AAAATTTCTT GGCTCTTACA CTAGCTATGA CTACTTGAAA 
AGGAACGTCA AGTGGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001355013.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341942.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 7, mRNA.

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CloneID	OHu85637
Clone ID Related Accession (Same CDS sequence)	XM_017022528.1 , NM_001355013.1
Accession Version	NM_001355013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022613.13, BC111712.1, BU618228.1, BX640879.1 and AF035298.1. On Sep 1, 2017 this sequence version replaced XM_017022528.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1803612.77932.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA 
AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG 
GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA 
CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT 
GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC 
GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341942.1
CDS	110..5389
Translation	MERNTFSMDCHSKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001355013.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355013.1

ORF Insert Sequence:

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA 
AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG 
GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA 
CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT 
GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC 
GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001355014.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341943.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA.

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CloneID	OHu85638
Clone ID Related Accession (Same CDS sequence)	XM_017022529.1 , NM_001355014.1 , NM_001355014.2
Accession Version	NM_001355014.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AC022613.13, BU618228.1, BX640879.1 and AF035298.1. On Sep 1, 2017 this sequence version replaced XM_017022529.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.97584.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT 
TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341943.1
CDS	476..5743
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001355014.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355014.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT 
TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001355014.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341943.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA.

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CloneID	OHu85638
Clone ID Related Accession (Same CDS sequence)	XM_017022529.1 , NM_001355014.1 , NM_001355014.2
Accession Version	NM_001355014.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AC022613.13, BU618228.1, BX640879.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_001355014.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.97584.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT 
TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341943.1
CDS	441..5708
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001355014.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355014.2

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT 
TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001355015.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341944.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA.

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CloneID	OHu85639
Clone ID Related Accession (Same CDS sequence)	XM_017022530.1 , NM_001355015.1 , NM_001355015.2
Accession Version	NM_001355015.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-12
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AK302971.1, AC022613.13, BM722482.1, BX640879.1 and AF035298.1. On Sep 1, 2017 this sequence version replaced XM_017022530.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.127.1, SRR1803614.6915.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341944.1
CDS	476..5542
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001355015.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355015.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001355015.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341944.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 9, mRNA.

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CloneID	OHu85639
Clone ID Related Accession (Same CDS sequence)	XM_017022530.1 , NM_001355015.1 , NM_001355015.2
Accession Version	NM_001355015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform i
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from L14837.1, BC111712.1, AK302971.1, AC022613.13, BM722482.1, BX640879.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_001355015.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.127.1, SRR1803614.260442.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341944.1
CDS	441..5507
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001355015.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355015.2

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017022525.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878014.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant X6, mRNA.

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CloneID	OHu85632
Clone ID Related Accession (Same CDS sequence)	XM_017022525.1
Accession Version	XM_017022525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA 
AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG 
GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA 
CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT 
GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAATTTCT TGGCTCTTAC ACTAGCTATG ACTACTTGAA AAGGAACGTC 
AAGTGGGGAA AGCCTCCTGA AGCTGATGGT GTGGATAGAT CATTTGGCGA GAAACGCTAT 
GAACCCATCC AGGCCACTCC CCCTCCTCCT CCATTGCCCT CGCAGTATGC CCAGCCATCT 
CAGCCTGTCA CCAGCGCGTC TCTCCACATA CATTCTAAGG GAGCACATGG TGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA GAATTCCTTA GTGTCCAAAC CAGACCCACC TCCATCTCAG 
AATAAGCCAG CAACTTTCAG ACCACCAAAC CGAGAAGATA CTGCTCAGGC AGCTTTCTAT 
CCCCAGAAAA GTTTTCCAGA TAAAGCCCCA GTTAATGGAA CTGAACAGAC TCAGAAAACA 
GTCACTCCAG CATACAATCG ATTCACACCA AAACCATATA CAAGTTCTGC CCGACCATTT 
GAACGCAAGT TTGAAAGTCC TAAATTCAAT CACAATCTTC TGCCAAGTGA AACTGCACAT 
AAACCTGACT TGTCTTCAAA AACTCCCACT TCTCCAAAAA CTCTTGTGAA ATCGCACAGT 
TTGGCACAGC CTCCTGAGTT TGACAGTGGA GTTGAAACTT TCTCTATCCA TGCAGAGAAG 
CCTAAATATC AAATAAATAA TATCAGCACA GTGCCTAAAG CTATTCCTGT GAGTCCTTCA 
GCTGTGGAAG AGGATGAAGA TGAAGATGGT CATACTGTGG TGGCCACAGC CCGAGGCATA 
TTTAACAGCA ATGGGGGCGT GCTGAGTTCC ATAGAAACTG GTGTTAGTAT AATTATCCCT 
CAAGGAGCCA TTCCCGAAGG AGTTGAGCAG GAAATCTATT TCAAGGTCTG CCGGGACAAC 
AGCATCCTTC CACCTTTAGA TAAAGAGAAA GGTGAAACAC TGCTGAGTCC TTTGGTGATG 
TGTGGTCCCC ATGGCCTCAA GTTCCTGAAG CCTGTGGAGC TGCGCTTACC ACACTGTGCG 
TCCATGACTC CTGACGGTTG GTCTTTTGCT CTAAAATCAT CCGACTCCTC GTCGGGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCC GGAGATCCAA ATTATCTCGT TGGAGCAAAC 
TGTGTTTCTG TCCTTATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878014.1
CDS	110..5479
Translation	MERNTFSMDCHSKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKFLGSYTSYDYLKRNVKWGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017022525.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022525.1

ORF Insert Sequence:

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA 
AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG 
GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA 
CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT 
GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAATTTCT TGGCTCTTAC ACTAGCTATG ACTACTTGAA AAGGAACGTC 
AAGTGGGGAA AGCCTCCTGA AGCTGATGGT GTGGATAGAT CATTTGGCGA GAAACGCTAT 
GAACCCATCC AGGCCACTCC CCCTCCTCCT CCATTGCCCT CGCAGTATGC CCAGCCATCT 
CAGCCTGTCA CCAGCGCGTC TCTCCACATA CATTCTAAGG GAGCACATGG TGAAGGTAAT 
TCAGTGTCAT TGGATTTTCA GAATTCCTTA GTGTCCAAAC CAGACCCACC TCCATCTCAG 
AATAAGCCAG CAACTTTCAG ACCACCAAAC CGAGAAGATA CTGCTCAGGC AGCTTTCTAT 
CCCCAGAAAA GTTTTCCAGA TAAAGCCCCA GTTAATGGAA CTGAACAGAC TCAGAAAACA 
GTCACTCCAG CATACAATCG ATTCACACCA AAACCATATA CAAGTTCTGC CCGACCATTT 
GAACGCAAGT TTGAAAGTCC TAAATTCAAT CACAATCTTC TGCCAAGTGA AACTGCACAT 
AAACCTGACT TGTCTTCAAA AACTCCCACT TCTCCAAAAA CTCTTGTGAA ATCGCACAGT 
TTGGCACAGC CTCCTGAGTT TGACAGTGGA GTTGAAACTT TCTCTATCCA TGCAGAGAAG 
CCTAAATATC AAATAAATAA TATCAGCACA GTGCCTAAAG CTATTCCTGT GAGTCCTTCA 
GCTGTGGAAG AGGATGAAGA TGAAGATGGT CATACTGTGG TGGCCACAGC CCGAGGCATA 
TTTAACAGCA ATGGGGGCGT GCTGAGTTCC ATAGAAACTG GTGTTAGTAT AATTATCCCT 
CAAGGAGCCA TTCCCGAAGG AGTTGAGCAG GAAATCTATT TCAAGGTCTG CCGGGACAAC 
AGCATCCTTC CACCTTTAGA TAAAGAGAAA GGTGAAACAC TGCTGAGTCC TTTGGTGATG 
TGTGGTCCCC ATGGCCTCAA GTTCCTGAAG CCTGTGGAGC TGCGCTTACC ACACTGTGCG 
TCCATGACTC CTGACGGTTG GTCTTTTGCT CTAAAATCAT CCGACTCCTC GTCGGGTGAT 
CCTAAAACCT GGCAAAACAA GTGTCTTCCC GGAGATCCAA ATTATCTCGT TGGAGCAAAC 
TGTGTTTCTG TCCTTATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017022526.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878015.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu85633
Clone ID Related Accession (Same CDS sequence)	XM_017022526.1
Accession Version	XM_017022526.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAATTTCTTG GCTCTTACAC TAGCTATGAC TACTTGAAAA GGAACGTCAA GTGGGGAAAG 
CCTCCTGAAG CTGATGGTGT GGATAGATCA TTTGGCGAGA AACGCTATGA ACCCATCCAG 
GCCACTCCCC CTCCTCCTCC ATTGCCCTCG CAGTATGCCC AGCCATCTCA GCCTGTCACC 
AGCGCGTCTC TCCACATACA TTCTAAGGGA GCACATGGTG AAGGTAATTC AGTGTCATTG 
GATTTTCAGA ATTCCTTAGT GTCCAAACCA GACCCACCTC CATCTCAGAA TAAGCCAGCA 
ACTTTCAGAC CACCAAACCG AGAAGATACT GCTCAGGCAG CTTTCTATCC CCAGAAAAGT 
TTTCCAGATA AAGCCCCAGT TAATGGAACT GAACAGACTC AGAAAACAGT CACTCCAGCA 
TACAATCGAT TCACACCAAA ACCATATACA AGTTCTGCCC GACCATTTGA ACGCAAGTTT 
GAAAGTCCTA AATTCAATCA CAATCTTCTG CCAAGTGAAA CTGCACATAA ACCTGACTTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTGTGAAAT CGCACAGTTT GGCACAGCCT 
CCTGAGTTTG ACAGTGGAGT TGAAACTTTC TCTATCCATG CAGAGAAGCC TAAATATCAA 
ATAAATAATA TCAGCACAGT GCCTAAAGCT ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG 
GATGAAGATG AAGATGGTCA TACTGTGGTG GCCACAGCCC GAGGCATATT TAACAGCAAT 
GGGGGCGTGC TGAGTTCCAT AGAAACTGGT GTTAGTATAA TTATCCCTCA AGGAGCCATT 
CCCGAAGGAG TTGAGCAGGA AATCTATTTC AAGGTCTGCC GGGACAACAG CATCCTTCCA 
CCTTTAGATA AAGAGAAAGG TGAAACACTG CTGAGTCCTT TGGTGATGTG TGGTCCCCAT 
GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG CGCTTACCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCCGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC 
CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878015.1
CDS	107..5464
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKFLGSYTSYDYLKRNVKWGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017022526.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022526.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAATTTCTTG GCTCTTACAC TAGCTATGAC TACTTGAAAA GGAACGTCAA GTGGGGAAAG 
CCTCCTGAAG CTGATGGTGT GGATAGATCA TTTGGCGAGA AACGCTATGA ACCCATCCAG 
GCCACTCCCC CTCCTCCTCC ATTGCCCTCG CAGTATGCCC AGCCATCTCA GCCTGTCACC 
AGCGCGTCTC TCCACATACA TTCTAAGGGA GCACATGGTG AAGGTAATTC AGTGTCATTG 
GATTTTCAGA ATTCCTTAGT GTCCAAACCA GACCCACCTC CATCTCAGAA TAAGCCAGCA 
ACTTTCAGAC CACCAAACCG AGAAGATACT GCTCAGGCAG CTTTCTATCC CCAGAAAAGT 
TTTCCAGATA AAGCCCCAGT TAATGGAACT GAACAGACTC AGAAAACAGT CACTCCAGCA 
TACAATCGAT TCACACCAAA ACCATATACA AGTTCTGCCC GACCATTTGA ACGCAAGTTT 
GAAAGTCCTA AATTCAATCA CAATCTTCTG CCAAGTGAAA CTGCACATAA ACCTGACTTG 
TCTTCAAAAA CTCCCACTTC TCCAAAAACT CTTGTGAAAT CGCACAGTTT GGCACAGCCT 
CCTGAGTTTG ACAGTGGAGT TGAAACTTTC TCTATCCATG CAGAGAAGCC TAAATATCAA 
ATAAATAATA TCAGCACAGT GCCTAAAGCT ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG 
GATGAAGATG AAGATGGTCA TACTGTGGTG GCCACAGCCC GAGGCATATT TAACAGCAAT 
GGGGGCGTGC TGAGTTCCAT AGAAACTGGT GTTAGTATAA TTATCCCTCA AGGAGCCATT 
CCCGAAGGAG TTGAGCAGGA AATCTATTTC AAGGTCTGCC GGGACAACAG CATCCTTCCA 
CCTTTAGATA AAGAGAAAGG TGAAACACTG CTGAGTCCTT TGGTGATGTG TGGTCCCCAT 
GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG CGCTTACCAC ACTGTGCGTC CATGACTCCT 
GACGGTTGGT CTTTTGCTCT AAAATCATCC GACTCCTCGT CGGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCCGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC 
CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017022527.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878016.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant X8, mRNA.

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CloneID	OHu85634
Clone ID Related Accession (Same CDS sequence)	XM_017022527.1
Accession Version	XM_017022527.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGTATT ACTTATTTAA GAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
ACAGTGACGC TTCACAGGGC TCCTGGATTT GGATTTGGAA TTGCAATATC TGGTGGACGA 
GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA 
GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA 
ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA 
AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA 
CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC 
CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT 
AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCTGCT 
AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG 
GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT 
ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG 
ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA 
GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT 
GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA 
TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT 
TCTGATCATT CCAGGCACTC GCCGCAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT 
GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC 
ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT 
CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAACCAG ATGTGGATTT ACCTGTCAGT 
CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG 
AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT 
GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG 
GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA 
GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG 
AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA 
ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG 
TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT 
GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG 
CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG 
AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC 
GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA 
TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA 
AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC 
CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA 
CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT 
CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG 
TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATACG AGCGATCTCA TAAACTTCGT 
AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG 
TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG 
GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC 
CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC 
TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT 
AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA 
GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG 
CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA 
GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC 
TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT 
CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT 
CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG 
TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA 
GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA 
CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG 
TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT 
CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT 
CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG 
TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC 
AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT 
CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG 
CCCAAGCCTG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA 
CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT 
GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA 
CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG 
TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG 
AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT 
GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAAT TCAGTGAACA TGACAAAACT 
CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATTGTTCGG 
TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT 
GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG 
CCTGCAAAGC CAGCGCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAATTT 
CTTGGCTCTT ACACTAGCTA TGACTACTTG AAAAGGAACG TCAAGTGGGG AAAGCCTCCT 
GAAGCTGATG GTGTGGATAG ATCATTTGGC GAGAAACGCT ATGAACCCAT CCAGGCCACT 
CCCCCTCCTC CTCCATTGCC CTCGCAGTAT GCCCAGCCAT CTCAGCCTGT CACCAGCGCG 
TCTCTCCACA TACATTCTAA GGGAGCACAT GGTGAAGGTA ATTCAGTGTC ATTGGATTTT 
CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC 
AGACCACCAA ACCGAGAAGA TACTGCTCAG GCAGCTTTCT ATCCCCAGAA AAGTTTTCCA 
GATAAAGCCC CAGTTAATGG AACTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT 
CGATTCACAC CAAAACCATA TACAAGTTCT GCCCGACCAT TTGAACGCAA GTTTGAAAGT 
CCTAAATTCA ATCACAATCT TCTGCCAAGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA 
AAAACTCCCA CTTCTCCAAA AACTCTTGTG AAATCGCACA GTTTGGCACA GCCTCCTGAG 
TTTGACAGTG GAGTTGAAAC TTTCTCTATC CATGCAGAGA AGCCTAAATA TCAAATAAAT 
AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTGTGGA AGAGGATGAA 
GATGAAGATG GTCATACTGT GGTGGCCACA GCCCGAGGCA TATTTAACAG CAATGGGGGC 
GTGCTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CTCAAGGAGC CATTCCCGAA 
GGAGTTGAGC AGGAAATCTA TTTCAAGGTC TGCCGGGACA ACAGCATCCT TCCACCTTTA 
GATAAAGAGA AAGGTGAAAC ACTGCTGAGT CCTTTGGTGA TGTGTGGTCC CCATGGCCTC 
AAGTTCCTGA AGCCTGTGGA GCTGCGCTTA CCACACTGTG CGTCCATGAC TCCTGACGGT 
TGGTCTTTTG CTCTAAAATC ATCCGACTCC TCGTCGGGTG ATCCTAAAAC CTGGCAAAAC 
AAGTGTCTTC CCGGAGATCC AAATTATCTC GTTGGAGCAA ACTGTGTTTC TGTCCTTATT 
GACCACTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878016.1
CDS	154..5505
Translation	MLYYLFKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKFLGSYTSYDYLKRNVKWGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017022527.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022527.1

ORF Insert Sequence:

ATGCTGTATT ACTTATTTAA GAGCACAGCA ATGGAGGAAA CAGCTATATG GGAACAACAT 
ACAGTGACGC TTCACAGGGC TCCTGGATTT GGATTTGGAA TTGCAATATC TGGTGGACGA 
GATAATCCTC ATTTTCAGAG TGGGGAAACG TCAATAGTGA TTTCAGATGT GCTGAAAGGA 
GGACCAGCTG AAGGACAGCT ACAGGAAAAT GACCGAGTTG CAATGGTTAA CGGAGTTTCA 
ATGGATAATG TTGAACATGC TTTTGCTGTT CAGCAACTAA GGAAAAGTGG GAAAAATGCA 
AAAATTACAA TTAGAAGGAA GAAGAAAGTT CAAATACCAG TAAGTCGTCC TGATCCTGAA 
CCAGTATCTG ATAATGAAGA AGATAGTTAT GATGAGGAAA TACATGATCC AAGAAGTGGC 
CGGAGTGGTG TGGTTAACAG AAGGAGTGAG AAGATTTGGC CGAGGGATAG AAGTGCAAGT 
AGAGAGAGGA GCTTGTCCCC GCGGTCAGAC AGGCGGTCAG TGGCTTCCAG CCAGCCTGCT 
AAACCTACTA AAGTCACACT GGTGAAATCC CGGAAAAATG AAGAATATGG TCTTCGATTG 
GCAAGCCATA TATTTGTTAA GGAAATTTCA CAAGATAGTT TGGCAGCAAG AGATGGCAAT 
ATTCAAGAAG GTGATGTTGT ATTGAAGATA AATGGTACTG TGACAGAAAA TATGTCATTG 
ACAGATGCAA AGACATTGAT AGAAAGGTCT AAAGGCAAAT TAAAAATGGT AGTTCAAAGA 
GATGAACGGG CTACGCTATT GAATGTCCCT GATCTTTCTG ACAGCATCCA CTCTGCTAAT 
GCCTCTGAGA GAGACGACAT TTCAGAAATT CAGTCACTGG CATCAGATCA TTCTGGTCGA 
TCACACGATA GGCCTCCCCG CCGCAGCCGG TCACGATCTC CTGACCAGCG GTCAGAGCCT 
TCTGATCATT CCAGGCACTC GCCGCAGCAG CCAAGCAATG GCAGTCTCCG GAGTAGAGAT 
GAAGAGAGAA TTTCTAAACC TGGGGCTGTC TCAACTCCTG TAAAGCATGC TGATGATCAC 
ACACCTAAAA CAGTGGAAGA AGTTACAGTT GAAAGAAATG AGAAACAAAC ACCTTCTCTT 
CCAGAACCAA AGCCTGTGTA TGCCCAAGTT GGGCAACCAG ATGTGGATTT ACCTGTCAGT 
CCATCTGATG GTGTCCTACC TAATTCAACT CATGAAGATG GGATTCTTCG GCCCAGCATG 
AAATTGGTAA AATTCAGAAA AGGAGATAGT GTGGGTTTGC GGCTGGCTGG TGGAAATGAT 
GTTGGAATAT TTGTAGCTGG CGTTCTAGAA GATAGCCCTG CAGCCAAGGA AGGCTTAGAG 
GAAGGTGATC AAATTCTCAG GGTAAACAAC GTAGATTTTA CAAATATCAT AAGAGAAGAA 
GCCGTCCTTT TCCTGCTTGA CCTCCCTAAA GGAGAAGAAG TGACCATATT GGCTCAGAAG 
AAGAAGGATG TTTATCGTCG CATTGTAGAA TCAGATGTAG GAGATTCTTT CTATATTAGA 
ACCCATTTTG AATATGAAAA GGAATCTCCC TATGGACTTA GTTTTAACAA AGGAGAGGTG 
TTCCGTGTTG TGGATACCTT GTACAATGGA AAACTGGGCT CTTGGCTTGC TATTCGAATT 
GGTAAAAATC ATAAGGAGGT AGAACGAGGC ATCATCCCTA ATAAGAACAG AGCTGAGCAG 
CTAGCCAGTG TACAGTATAC ACTTCCAAAA ACAGCAGGCG GAGACCGTGC TGACTTCTGG 
AGATTCAGAG GTCTTCGCAG CTCCAAGAGA AATCTTCGAA AAAGCAGAGA GGATTTGTCC 
GCTCAGCCTG TTCAAACAAA GTTTCCAGCT TATGAAAGAG TGGTTCTTCG AGAAGCTGGA 
TTTCTGAGGC CTGTAACCAT TTTTGGACCA ATAGCTGATG TTGCCAGAGA AAAGCTGGCA 
AGAGAAGAAC CAGATATTTA TCAAATTGCA AAGAGTGAAC CACGAGACGC TGGAACTGAC 
CAACGTAGCT CTGGCATTAT TCGCCTGCAT ACAATAAAGC AAATCATAGA TCAAGACAAA 
CATGCTTTAT TAGATGTAAC ACCAAATGCA GTTGATCGTC TTAACTATGC CCAGTGGTAT 
CCAATTGTTG TATTTCTTAA CCCTGATTCT AAGCAAGGAG TAAAAACAAT GAGAATGAGG 
TTATGTCCAG AATCTCGGAA AAGTGCCAGG AAGTTATACG AGCGATCTCA TAAACTTCGT 
AAAAATAATC ACCATCTTTT TACAACTACA ATTAACTTAA ATTCAATGAA TGATGGTTGG 
TATGGTGCGC TGAAAGAAGC AATTCAACAA CAGCAAAACC AGCTGGTATG GGTTTCCGAG 
GGAAAGGCGG ATGGTGCTAC AAGTGATGAC CTTGATTTGC ATGATGATCG TCTGTCCTAC 
CTGTCAGCTC CAGGTAGTGA ATACTCAATG TATAGCACGG ACAGTAGACA CACTTCTGAC 
TATGAAGACA CAGACACAGA AGGCGGGGCC TACACTGATC AAGAACTAGA TGAAACTCTT 
AATGATGAGG TTGGGACTCC ACCGGAGTCT GCCATTACAC GGTCCTCTGA GCCTGTAAGA 
GAGGACTCCT CTGGAATGCA TCATGAAAAC CAAACATATC CTCCTTACTC ACCACAAGCG 
CAGCCACAAC CAATTCATAG AATAGACTCC CCTGGATTTA AGCCAGCCTC TCAACAGAAA 
GCAGAAGCTT CATCTCCAGT CCCTTACCTT TCGCCTGAAA CAAACCCAGC ATCATCAACC 
TCTGCTGTTA ATCATAATGT AAATTTAACT AATGTCAGAC TGGAGGAGCC CACCCCAGCT 
CCTTCCACCT CTTACTCACC ACAAGCTGAT TCTTTAAGAA CACCAAGTAC TGAGGCAGCT 
CACATAATGC TAAGAGATCA AGAACCATCA TTGTCGTCGC ATGTAGATCC AACAAAGGTG 
TATAGAAAGG ATCCATATCC CGAGGAAATG ATGAGGCAGA ACCATGTTTT GAAACAGCCA 
GCCGTTAGTC ACCCAGGGCA CAGGCCAGAC AAAGAGCCTA ATCTGACCTA TGAACCCCAA 
CTCCCATACG TAGAGAAACA AGCCAGCAGA GACCTCGAGC AGCCCACATA CAGATACGAG 
TCCTCAAGCT ATACGGACCA GTTTTCTCGA AACTATGAAC ATCGTCTGCG ATACGAAGAT 
CGCGTCCCCA TGTATGAAGA ACAGTGGTCA TATTATGATG ACAAACAGCC CTACCCATCT 
CGGCCACCTT TTGATAATCA GCACTCTCAA GACCTTGACT CCAGACAGCA TCCCGAAGAG 
TCCTCAGAAC GAGGGTACTT TCCACGTTTT GAAGAGCCAG CCCCTCTGTC TTACGACAGC 
AGACCACGTT ACGAACAGGC ACCTAGAGCA TCCGCCCTGC GGCACGAAGA GCAGCCAGCT 
CCTGGGTATG ACACACATGG TAGACTCAGA CCGGAAGCCC AGCCCCACCC TTCAGCAGGG 
CCCAAGCCTG CAGAGTCCAA GCAGTATTTT GAGCAATATT CACGCAGTTA CGAGCAAGTA 
CCACCCCAAG GATTTACCTC TAGAGCAGGT CATTTTGAGC CTCTCCATGG TGCTGCAGCT 
GTCCCTCCGC TGATACCTTC ATCTCAGCAT AAGCCAGAAG CTCTGCCTTC AAACACCAAA 
CCACTGCCTC CACCCCCAAC TCAAACCGAA GAAGAGGAAG ATCCAGCAAT GAAGCCACAG 
TCTGTACTCA CCAGAGTTAA GATGTTTGAA AACAAAAGAT CTGCATCCTT AGAGACCAAG 
AAGGATGTAA ATGACACTGG CAGTTTTAAG CCTCCAGAAG TAGCATCTAA ACCTTCAGGT 
GCTCCCATCA TTGGTCCCAA ACCCACTTCT CAGAATCAAT TCAGTGAACA TGACAAAACT 
CTGTACAGGA TCCCAGAACC TCAAAAACCT CAACTGAAGC CACCTGAAGA TATTGTTCGG 
TCCAATCATT ATGACCCTGA AGAAGATGAA GAATATTATC GAAAACAGCT GTCATACTTT 
GACCGAAGAA GTTTTGAGAA TAAGCCTCCT GCACACATTG CCGCCAGCCA TCTCTCCGAG 
CCTGCAAAGC CAGCGCATTC TCAGAATCAA TCAAATTTTT CTAGTTATTC TTCAAAATTT 
CTTGGCTCTT ACACTAGCTA TGACTACTTG AAAAGGAACG TCAAGTGGGG AAAGCCTCCT 
GAAGCTGATG GTGTGGATAG ATCATTTGGC GAGAAACGCT ATGAACCCAT CCAGGCCACT 
CCCCCTCCTC CTCCATTGCC CTCGCAGTAT GCCCAGCCAT CTCAGCCTGT CACCAGCGCG 
TCTCTCCACA TACATTCTAA GGGAGCACAT GGTGAAGGTA ATTCAGTGTC ATTGGATTTT 
CAGAATTCCT TAGTGTCCAA ACCAGACCCA CCTCCATCTC AGAATAAGCC AGCAACTTTC 
AGACCACCAA ACCGAGAAGA TACTGCTCAG GCAGCTTTCT ATCCCCAGAA AAGTTTTCCA 
GATAAAGCCC CAGTTAATGG AACTGAACAG ACTCAGAAAA CAGTCACTCC AGCATACAAT 
CGATTCACAC CAAAACCATA TACAAGTTCT GCCCGACCAT TTGAACGCAA GTTTGAAAGT 
CCTAAATTCA ATCACAATCT TCTGCCAAGT GAAACTGCAC ATAAACCTGA CTTGTCTTCA 
AAAACTCCCA CTTCTCCAAA AACTCTTGTG AAATCGCACA GTTTGGCACA GCCTCCTGAG 
TTTGACAGTG GAGTTGAAAC TTTCTCTATC CATGCAGAGA AGCCTAAATA TCAAATAAAT 
AATATCAGCA CAGTGCCTAA AGCTATTCCT GTGAGTCCTT CAGCTGTGGA AGAGGATGAA 
GATGAAGATG GTCATACTGT GGTGGCCACA GCCCGAGGCA TATTTAACAG CAATGGGGGC 
GTGCTGAGTT CCATAGAAAC TGGTGTTAGT ATAATTATCC CTCAAGGAGC CATTCCCGAA 
GGAGTTGAGC AGGAAATCTA TTTCAAGGTC TGCCGGGACA ACAGCATCCT TCCACCTTTA 
GATAAAGAGA AAGGTGAAAC ACTGCTGAGT CCTTTGGTGA TGTGTGGTCC CCATGGCCTC 
AAGTTCCTGA AGCCTGTGGA GCTGCGCTTA CCACACTGTG CGTCCATGAC TCCTGACGGT 
TGGTCTTTTG CTCTAAAATC ATCCGACTCC TCGTCGGGTG ATCCTAAAAC CTGGCAAAAC 
AAGTGTCTTC CCGGAGATCC AAATTATCTC GTTGGAGCAA ACTGTGTTTC TGTCCTTATT 
GACCACTTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017022528.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878017.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant X13, mRNA.

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CloneID	OHu85637
Clone ID Related Accession (Same CDS sequence)	XM_017022528.1 , NM_001355013.1
Accession Version	XM_017022528.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5280bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	tight junction protein ZO-1 isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA 
AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG 
GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA 
CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT 
GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC 
GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878017.1
CDS	110..5389
Translation	MERNTFSMDCHSKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017022528.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022528.1

ORF Insert Sequence:

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGAAAGC AGAAGCTTCA TCTCCAGTCC CTTACCTTTC GCCTGAAACA 
AACCCAGCAT CATCAACCTC TGCTGTTAAT CATAATGTAA ATTTAACTAA TGTCAGACTG 
GAGGAGCCCA CCCCAGCTCC TTCCACCTCT TACTCACCAC AAGCTGATTC TTTAAGAACA 
CCAAGTACTG AGGCAGCTCA CATAATGCTA AGAGATCAAG AACCATCATT GTCGTCGCAT 
GTAGATCCAA CAAAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTGAT CCTAAAACCT GGCAAAACAA GTGTCTTCCC 
GGAGATCCAA ATTATCTCGT TGGAGCAAAC TGTGTTTCTG TCCTTATTGA CCACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017022529.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878018.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant X14, mRNA.

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CloneID	OHu85638
Clone ID Related Accession (Same CDS sequence)	XM_017022529.1 , NM_001355014.1 , NM_001355014.2
Accession Version	XM_017022529.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5268bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	tight junction protein ZO-1 isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT 
TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878018.1
CDS	107..5374
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017022529.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022529.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT 
TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017022530.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878019.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant X15, mRNA.

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CloneID	OHu85639
Clone ID Related Accession (Same CDS sequence)	XM_017022530.1 , NM_001355015.1 , NM_001355015.2
Accession Version	XM_017022530.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5067bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	tight junction protein ZO-1 isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878019.1
CDS	107..5173
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017022530.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022530.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001301025.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287954.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA.

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CloneID	OHu48519
Clone ID Related Accession (Same CDS sequence)	NM_001301025.1
Accession Version	NM_001301025.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5508bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-22
Organism	Homo sapiens(Human)
Product	tight junction protein ZO-1 isoform c
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CN372462.1, AB370197.1, AC022613.13 and AF035298.1. On or before Jul 18, 2014 this sequence version replaced XM_006725553.1, XM_005254616.1. Transcript Variant: This variant (3) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, and it uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is longer than isoform a. The exon combination of this variant is inferred based on partial transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAAGTACC AGAAATACCT GACGGTGCTG CAGATGGCCA TCGGCGTCAC CCCCTCCAAC 
CGCGGCAGCC TCCTGCCGCT CAAGAGGAAG CTGTGGGTAA CGCCATCCTC TGAAAATCCT 
AATGGTGCTA CTTCTAGTGT CAGCCAAGGA AAACCCTCTT TAAGACGAAT TAAAGGGAGA 
TTACACAGAA GCAAAAGCCT TGATAGCATG GATTTCTGTG AGCTCACTAG CACAGCAATG 
GAGGAAACAG CTATATGGGA ACAACATACA GTGACGCTTC ACAGGGCTCC TGGATTTGGA 
TTTGGAATTG CAATATCTGG TGGACGAGAT AATCCTCATT TTCAGAGTGG GGAAACGTCA 
ATAGTGATTT CAGATGTGCT GAAAGGAGGA CCAGCTGAAG GACAGCTACA GGAAAATGAC 
CGAGTTGCAA TGGTTAACGG AGTTTCAATG GATAATGTTG AACATGCTTT TGCTGTTCAG 
CAACTAAGGA AAAGTGGGAA AAATGCAAAA ATTACAATTA GAAGGAAGAA GAAAGTTCAA 
ATACCAGTAA GTCGTCCTGA TCCTGAACCA GTATCTGATA ATGAAGAAGA TAGTTATGAT 
GAGGAAATAC ATGATCCAAG AAGTGGCCGG AGTGGTGTGG TTAACAGAAG GAGTGAGAAG 
ATTTGGCCGA GGGATAGAAG TGCAAGTAGA GAGAGGAGCT TGTCCCCGCG GTCAGACAGG 
CGGTCAGTGG CTTCCAGCCA GCCTGCTAAA CCTACTAAAG TCACACTGGT GAAATCCCGG 
AAAAATGAAG AATATGGTCT TCGATTGGCA AGCCATATAT TTGTTAAGGA AATTTCACAA 
GATAGTTTGG CAGCAAGAGA TGGCAATATT CAAGAAGGTG ATGTTGTATT GAAGATAAAT 
GGTACTGTGA CAGAAAATAT GTCATTGACA GATGCAAAGA CATTGATAGA AAGGTCTAAA 
GGCAAATTAA AAATGGTAGT TCAAAGAGAT GAACGGGCTA CGCTATTGAA TGTCCCTGAT 
CTTTCTGACA GCATCCACTC TGCTAATGCC TCTGAGAGAG ACGACATTTC AGAAATTCAG 
TCACTGGCAT CAGATCATTC TGGTCGATCA CACGATAGGC CTCCCCGCCG CAGCCGGTCA 
CGATCTCCTG ACCAGCGGTC AGAGCCTTCT GATCATTCCA GGCACTCGCC GCAGCAGCCA 
AGCAATGGCA GTCTCCGGAG TAGAGATGAA GAGAGAATTT CTAAACCTGG GGCTGTCTCA 
ACTCCTGTAA AGCATGCTGA TGATCACACA CCTAAAACAG TGGAAGAAGT TACAGTTGAA 
AGAAATGAGA AACAAACACC TTCTCTTCCA GAACCAAAGC CTGTGTATGC CCAAGTTGGG 
CAACCAGATG TGGATTTACC TGTCAGTCCA TCTGATGGTG TCCTACCTAA TTCAACTCAT 
GAAGATGGGA TTCTTCGGCC CAGCATGAAA TTGGTAAAAT TCAGAAAAGG AGATAGTGTG 
GGTTTGCGGC TGGCTGGTGG AAATGATGTT GGAATATTTG TAGCTGGCGT TCTAGAAGAT 
AGCCCTGCAG CCAAGGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT AAACAACGTA 
GATTTTACAA ATATCATAAG AGAAGAAGCC GTCCTTTTCC TGCTTGACCT CCCTAAAGGA 
GAAGAAGTGA CCATATTGGC TCAGAAGAAG AAGGATGTTT ATCGTCGCAT TGTAGAATCA 
GATGTAGGAG ATTCTTTCTA TATTAGAACC CATTTTGAAT ATGAAAAGGA ATCTCCCTAT 
GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGTGTTGTGG ATACCTTGTA CAATGGAAAA 
CTGGGCTCTT GGCTTGCTAT TCGAATTGGT AAAAATCATA AGGAGGTAGA ACGAGGCATC 
ATCCCTAATA AGAACAGAGC TGAGCAGCTA GCCAGTGTAC AGTATACACT TCCAAAAACA 
GCAGGCGGAG ACCGTGCTGA CTTCTGGAGA TTCAGAGGTC TTCGCAGCTC CAAGAGAAAT 
CTTCGAAAAA GCAGAGAGGA TTTGTCCGCT CAGCCTGTTC AAACAAAGTT TCCAGCTTAT 
GAAAGAGTGG TTCTTCGAGA AGCTGGATTT CTGAGGCCTG TAACCATTTT TGGACCAATA 
GCTGATGTTG CCAGAGAAAA GCTGGCAAGA GAAGAACCAG ATATTTATCA AATTGCAAAG 
AGTGAACCAC GAGACGCTGG AACTGACCAA CGTAGCTCTG GCATTATTCG CCTGCATACA 
ATAAAGCAAA TCATAGATCA AGACAAACAT GCTTTATTAG ATGTAACACC AAATGCAGTT 
GATCGTCTTA ACTATGCCCA GTGGTATCCA ATTGTTGTAT TTCTTAACCC TGATTCTAAG 
CAAGGAGTAA AAACAATGAG AATGAGGTTA TGTCCAGAAT CTCGGAAAAG TGCCAGGAAG 
TTATACGAGC GATCTCATAA ACTTCGTAAA AATAATCACC ATCTTTTTAC AACTACAATT 
AACTTAAATT CAATGAATGA TGGTTGGTAT GGTGCGCTGA AAGAAGCAAT TCAACAACAG 
CAAAACCAGC TGGTATGGGT TTCCGAGGGA AAGGCGGATG GTGCTACAAG TGATGACCTT 
GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCTCCAG GTAGTGAATA CTCAATGTAT 
AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ACACAGAAGG CGGGGCCTAC 
ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTTG GGACTCCACC GGAGTCTGCC 
ATTACACGGT CCTCTGAGCC TGTAAGAGAG GACTCCTCTG GAATGCATCA TGAAAACCAA 
ACATATCCTC CTTACTCACC ACAAGCGCAG CCACAACCAA TTCATAGAAT AGACTCCCCT 
GGATTTAAGC CAGCCTCTCA ACAGAAAGCA GAAGCTTCAT CTCCAGTCCC TTACCTTTCG 
CCTGAAACAA ACCCAGCATC ATCAACCTCT GCTGTTAATC ATAATGTAAA TTTAACTAAT 
GTCAGACTGG AGGAGCCCAC CCCAGCTCCT TCCACCTCTT ACTCACCACA AGCTGATTCT 
TTAAGAACAC CAAGTACTGA GGCAGCTCAC ATAATGCTAA GAGATCAAGA ACCATCATTG 
TCGTCGCATG TAGATCCAAC AAAGGTGTAT AGAAAGGATC CATATCCCGA GGAAATGATG 
AGGCAGAACC ATGTTTTGAA ACAGCCAGCC GTTAGTCACC CAGGGCACAG GCCAGACAAA 
GAGCCTAATC TGACCTATGA ACCCCAACTC CCATACGTAG AGAAACAAGC CAGCAGAGAC 
CTCGAGCAGC CCACATACAG ATACGAGTCC TCAAGCTATA CGGACCAGTT TTCTCGAAAC 
TATGAACATC GTCTGCGATA CGAAGATCGC GTCCCCATGT ATGAAGAACA GTGGTCATAT 
TATGATGACA AACAGCCCTA CCCATCTCGG CCACCTTTTG ATAATCAGCA CTCTCAAGAC 
CTTGACTCCA GACAGCATCC CGAAGAGTCC TCAGAACGAG GGTACTTTCC ACGTTTTGAA 
GAGCCAGCCC CTCTGTCTTA CGACAGCAGA CCACGTTACG AACAGGCACC TAGAGCATCC 
GCCCTGCGGC ACGAAGAGCA GCCAGCTCCT GGGTATGACA CACATGGTAG ACTCAGACCG 
GAAGCCCAGC CCCACCCTTC AGCAGGGCCC AAGCCTGCAG AGTCCAAGCA GTATTTTGAG 
CAATATTCAC GCAGTTACGA GCAAGTACCA CCCCAAGGAT TTACCTCTAG AGCAGGTCAT 
TTTGAGCCTC TCCATGGTGC TGCAGCTGTC CCTCCGCTGA TACCTTCATC TCAGCATAAG 
CCAGAAGCTC TGCCTTCAAA CACCAAACCA CTGCCTCCAC CCCCAACTCA AACCGAAGAA 
GAGGAAGATC CAGCAATGAA GCCACAGTCT GTACTCACCA GAGTTAAGAT GTTTGAAAAC 
AAAAGATCTG CATCCTTAGA GACCAAGAAG GATGTAAATG ACACTGGCAG TTTTAAGCCT 
CCAGAAGTAG CATCTAAACC TTCAGGTGCT CCCATCATTG GTCCCAAACC CACTTCTCAG 
AATCAATTCA GTGAACATGA CAAAACTCTG TACAGGATCC CAGAACCTCA AAAACCTCAA 
CTGAAGCCAC CTGAAGATAT TGTTCGGTCC AATCATTATG ACCCTGAAGA AGATGAAGAA 
TATTATCGAA AACAGCTGTC ATACTTTGAC CGAAGAAGTT TTGAGAATAA GCCTCCTGCA 
CACATTGCCG CCAGCCATCT CTCCGAGCCT GCAAAGCCAG CGCATTCTCA GAATCAATCA 
AATTTTTCTA GTTATTCTTC AAAGGGAAAG CCTCCTGAAG CTGATGGTGT GGATAGATCA 
TTTGGCGAGA AACGCTATGA ACCCATCCAG GCCACTCCCC CTCCTCCTCC ATTGCCCTCG 
CAGTATGCCC AGCCATCTCA GCCTGTCACC AGCGCGTCTC TCCACATACA TTCTAAGGGA 
GCACATGGTG AAGGTAATTC AGTGTCATTG GATTTTCAGA ATTCCTTAGT GTCCAAACCA 
GACCCACCTC CATCTCAGAA TAAGCCAGCA ACTTTCAGAC CACCAAACCG AGAAGATACT 
GCTCAGGCAG CTTTCTATCC CCAGAAAAGT TTTCCAGATA AAGCCCCAGT TAATGGAACT 
GAACAGACTC AGAAAACAGT CACTCCAGCA TACAATCGAT TCACACCAAA ACCATATACA 
AGTTCTGCCC GACCATTTGA ACGCAAGTTT GAAAGTCCTA AATTCAATCA CAATCTTCTG 
CCAAGTGAAA CTGCACATAA ACCTGACTTG TCTTCAAAAA CTCCCACTTC TCCAAAAACT 
CTTGTGAAAT CGCACAGTTT GGCACAGCCT CCTGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTATCCATG CAGAGAAGCC TAAATATCAA ATAAATAATA TCAGCACAGT GCCTAAAGCT 
ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG GATGAAGATG AAGATGGTCA TACTGTGGTG 
GCCACAGCCC GAGGCATATT TAACAGCAAT GGGGGCGTGC TGAGTTCCAT AGAAACTGGT 
GTTAGTATAA TTATCCCTCA AGGAGCCATT CCCGAAGGAG TTGAGCAGGA AATCTATTTC 
AAGGTCTGCC GGGACAACAG CATCCTTCCA CCTTTAGATA AAGAGAAAGG TGAAACACTG 
CTGAGTCCTT TGGTGATGTG TGGTCCCCAT GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG 
CGCTTACCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC 
GACTCCTCGT CGGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT 
TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287954.1
CDS	39..5546
Translation	MKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001301025.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301025.1

ORF Insert Sequence:

ATGAAGTACC AGAAATACCT GACGGTGCTG CAGATGGCCA TCGGCGTCAC CCCCTCCAAC 
CGCGGCAGCC TCCTGCCGCT CAAGAGGAAG CTGTGGGTAA CGCCATCCTC TGAAAATCCT 
AATGGTGCTA CTTCTAGTGT CAGCCAAGGA AAACCCTCTT TAAGACGAAT TAAAGGGAGA 
TTACACAGAA GCAAAAGCCT TGATAGCATG GATTTCTGTG AGCTCACTAG CACAGCAATG 
GAGGAAACAG CTATATGGGA ACAACATACA GTGACGCTTC ACAGGGCTCC TGGATTTGGA 
TTTGGAATTG CAATATCTGG TGGACGAGAT AATCCTCATT TTCAGAGTGG GGAAACGTCA 
ATAGTGATTT CAGATGTGCT GAAAGGAGGA CCAGCTGAAG GACAGCTACA GGAAAATGAC 
CGAGTTGCAA TGGTTAACGG AGTTTCAATG GATAATGTTG AACATGCTTT TGCTGTTCAG 
CAACTAAGGA AAAGTGGGAA AAATGCAAAA ATTACAATTA GAAGGAAGAA GAAAGTTCAA 
ATACCAGTAA GTCGTCCTGA TCCTGAACCA GTATCTGATA ATGAAGAAGA TAGTTATGAT 
GAGGAAATAC ATGATCCAAG AAGTGGCCGG AGTGGTGTGG TTAACAGAAG GAGTGAGAAG 
ATTTGGCCGA GGGATAGAAG TGCAAGTAGA GAGAGGAGCT TGTCCCCGCG GTCAGACAGG 
CGGTCAGTGG CTTCCAGCCA GCCTGCTAAA CCTACTAAAG TCACACTGGT GAAATCCCGG 
AAAAATGAAG AATATGGTCT TCGATTGGCA AGCCATATAT TTGTTAAGGA AATTTCACAA 
GATAGTTTGG CAGCAAGAGA TGGCAATATT CAAGAAGGTG ATGTTGTATT GAAGATAAAT 
GGTACTGTGA CAGAAAATAT GTCATTGACA GATGCAAAGA CATTGATAGA AAGGTCTAAA 
GGCAAATTAA AAATGGTAGT TCAAAGAGAT GAACGGGCTA CGCTATTGAA TGTCCCTGAT 
CTTTCTGACA GCATCCACTC TGCTAATGCC TCTGAGAGAG ACGACATTTC AGAAATTCAG 
TCACTGGCAT CAGATCATTC TGGTCGATCA CACGATAGGC CTCCCCGCCG CAGCCGGTCA 
CGATCTCCTG ACCAGCGGTC AGAGCCTTCT GATCATTCCA GGCACTCGCC GCAGCAGCCA 
AGCAATGGCA GTCTCCGGAG TAGAGATGAA GAGAGAATTT CTAAACCTGG GGCTGTCTCA 
ACTCCTGTAA AGCATGCTGA TGATCACACA CCTAAAACAG TGGAAGAAGT TACAGTTGAA 
AGAAATGAGA AACAAACACC TTCTCTTCCA GAACCAAAGC CTGTGTATGC CCAAGTTGGG 
CAACCAGATG TGGATTTACC TGTCAGTCCA TCTGATGGTG TCCTACCTAA TTCAACTCAT 
GAAGATGGGA TTCTTCGGCC CAGCATGAAA TTGGTAAAAT TCAGAAAAGG AGATAGTGTG 
GGTTTGCGGC TGGCTGGTGG AAATGATGTT GGAATATTTG TAGCTGGCGT TCTAGAAGAT 
AGCCCTGCAG CCAAGGAAGG CTTAGAGGAA GGTGATCAAA TTCTCAGGGT AAACAACGTA 
GATTTTACAA ATATCATAAG AGAAGAAGCC GTCCTTTTCC TGCTTGACCT CCCTAAAGGA 
GAAGAAGTGA CCATATTGGC TCAGAAGAAG AAGGATGTTT ATCGTCGCAT TGTAGAATCA 
GATGTAGGAG ATTCTTTCTA TATTAGAACC CATTTTGAAT ATGAAAAGGA ATCTCCCTAT 
GGACTTAGTT TTAACAAAGG AGAGGTGTTC CGTGTTGTGG ATACCTTGTA CAATGGAAAA 
CTGGGCTCTT GGCTTGCTAT TCGAATTGGT AAAAATCATA AGGAGGTAGA ACGAGGCATC 
ATCCCTAATA AGAACAGAGC TGAGCAGCTA GCCAGTGTAC AGTATACACT TCCAAAAACA 
GCAGGCGGAG ACCGTGCTGA CTTCTGGAGA TTCAGAGGTC TTCGCAGCTC CAAGAGAAAT 
CTTCGAAAAA GCAGAGAGGA TTTGTCCGCT CAGCCTGTTC AAACAAAGTT TCCAGCTTAT 
GAAAGAGTGG TTCTTCGAGA AGCTGGATTT CTGAGGCCTG TAACCATTTT TGGACCAATA 
GCTGATGTTG CCAGAGAAAA GCTGGCAAGA GAAGAACCAG ATATTTATCA AATTGCAAAG 
AGTGAACCAC GAGACGCTGG AACTGACCAA CGTAGCTCTG GCATTATTCG CCTGCATACA 
ATAAAGCAAA TCATAGATCA AGACAAACAT GCTTTATTAG ATGTAACACC AAATGCAGTT 
GATCGTCTTA ACTATGCCCA GTGGTATCCA ATTGTTGTAT TTCTTAACCC TGATTCTAAG 
CAAGGAGTAA AAACAATGAG AATGAGGTTA TGTCCAGAAT CTCGGAAAAG TGCCAGGAAG 
TTATACGAGC GATCTCATAA ACTTCGTAAA AATAATCACC ATCTTTTTAC AACTACAATT 
AACTTAAATT CAATGAATGA TGGTTGGTAT GGTGCGCTGA AAGAAGCAAT TCAACAACAG 
CAAAACCAGC TGGTATGGGT TTCCGAGGGA AAGGCGGATG GTGCTACAAG TGATGACCTT 
GATTTGCATG ATGATCGTCT GTCCTACCTG TCAGCTCCAG GTAGTGAATA CTCAATGTAT 
AGCACGGACA GTAGACACAC TTCTGACTAT GAAGACACAG ACACAGAAGG CGGGGCCTAC 
ACTGATCAAG AACTAGATGA AACTCTTAAT GATGAGGTTG GGACTCCACC GGAGTCTGCC 
ATTACACGGT CCTCTGAGCC TGTAAGAGAG GACTCCTCTG GAATGCATCA TGAAAACCAA 
ACATATCCTC CTTACTCACC ACAAGCGCAG CCACAACCAA TTCATAGAAT AGACTCCCCT 
GGATTTAAGC CAGCCTCTCA ACAGAAAGCA GAAGCTTCAT CTCCAGTCCC TTACCTTTCG 
CCTGAAACAA ACCCAGCATC ATCAACCTCT GCTGTTAATC ATAATGTAAA TTTAACTAAT 
GTCAGACTGG AGGAGCCCAC CCCAGCTCCT TCCACCTCTT ACTCACCACA AGCTGATTCT 
TTAAGAACAC CAAGTACTGA GGCAGCTCAC ATAATGCTAA GAGATCAAGA ACCATCATTG 
TCGTCGCATG TAGATCCAAC AAAGGTGTAT AGAAAGGATC CATATCCCGA GGAAATGATG 
AGGCAGAACC ATGTTTTGAA ACAGCCAGCC GTTAGTCACC CAGGGCACAG GCCAGACAAA 
GAGCCTAATC TGACCTATGA ACCCCAACTC CCATACGTAG AGAAACAAGC CAGCAGAGAC 
CTCGAGCAGC CCACATACAG ATACGAGTCC TCAAGCTATA CGGACCAGTT TTCTCGAAAC 
TATGAACATC GTCTGCGATA CGAAGATCGC GTCCCCATGT ATGAAGAACA GTGGTCATAT 
TATGATGACA AACAGCCCTA CCCATCTCGG CCACCTTTTG ATAATCAGCA CTCTCAAGAC 
CTTGACTCCA GACAGCATCC CGAAGAGTCC TCAGAACGAG GGTACTTTCC ACGTTTTGAA 
GAGCCAGCCC CTCTGTCTTA CGACAGCAGA CCACGTTACG AACAGGCACC TAGAGCATCC 
GCCCTGCGGC ACGAAGAGCA GCCAGCTCCT GGGTATGACA CACATGGTAG ACTCAGACCG 
GAAGCCCAGC CCCACCCTTC AGCAGGGCCC AAGCCTGCAG AGTCCAAGCA GTATTTTGAG 
CAATATTCAC GCAGTTACGA GCAAGTACCA CCCCAAGGAT TTACCTCTAG AGCAGGTCAT 
TTTGAGCCTC TCCATGGTGC TGCAGCTGTC CCTCCGCTGA TACCTTCATC TCAGCATAAG 
CCAGAAGCTC TGCCTTCAAA CACCAAACCA CTGCCTCCAC CCCCAACTCA AACCGAAGAA 
GAGGAAGATC CAGCAATGAA GCCACAGTCT GTACTCACCA GAGTTAAGAT GTTTGAAAAC 
AAAAGATCTG CATCCTTAGA GACCAAGAAG GATGTAAATG ACACTGGCAG TTTTAAGCCT 
CCAGAAGTAG CATCTAAACC TTCAGGTGCT CCCATCATTG GTCCCAAACC CACTTCTCAG 
AATCAATTCA GTGAACATGA CAAAACTCTG TACAGGATCC CAGAACCTCA AAAACCTCAA 
CTGAAGCCAC CTGAAGATAT TGTTCGGTCC AATCATTATG ACCCTGAAGA AGATGAAGAA 
TATTATCGAA AACAGCTGTC ATACTTTGAC CGAAGAAGTT TTGAGAATAA GCCTCCTGCA 
CACATTGCCG CCAGCCATCT CTCCGAGCCT GCAAAGCCAG CGCATTCTCA GAATCAATCA 
AATTTTTCTA GTTATTCTTC AAAGGGAAAG CCTCCTGAAG CTGATGGTGT GGATAGATCA 
TTTGGCGAGA AACGCTATGA ACCCATCCAG GCCACTCCCC CTCCTCCTCC ATTGCCCTCG 
CAGTATGCCC AGCCATCTCA GCCTGTCACC AGCGCGTCTC TCCACATACA TTCTAAGGGA 
GCACATGGTG AAGGTAATTC AGTGTCATTG GATTTTCAGA ATTCCTTAGT GTCCAAACCA 
GACCCACCTC CATCTCAGAA TAAGCCAGCA ACTTTCAGAC CACCAAACCG AGAAGATACT 
GCTCAGGCAG CTTTCTATCC CCAGAAAAGT TTTCCAGATA AAGCCCCAGT TAATGGAACT 
GAACAGACTC AGAAAACAGT CACTCCAGCA TACAATCGAT TCACACCAAA ACCATATACA 
AGTTCTGCCC GACCATTTGA ACGCAAGTTT GAAAGTCCTA AATTCAATCA CAATCTTCTG 
CCAAGTGAAA CTGCACATAA ACCTGACTTG TCTTCAAAAA CTCCCACTTC TCCAAAAACT 
CTTGTGAAAT CGCACAGTTT GGCACAGCCT CCTGAGTTTG ACAGTGGAGT TGAAACTTTC 
TCTATCCATG CAGAGAAGCC TAAATATCAA ATAAATAATA TCAGCACAGT GCCTAAAGCT 
ATTCCTGTGA GTCCTTCAGC TGTGGAAGAG GATGAAGATG AAGATGGTCA TACTGTGGTG 
GCCACAGCCC GAGGCATATT TAACAGCAAT GGGGGCGTGC TGAGTTCCAT AGAAACTGGT 
GTTAGTATAA TTATCCCTCA AGGAGCCATT CCCGAAGGAG TTGAGCAGGA AATCTATTTC 
AAGGTCTGCC GGGACAACAG CATCCTTCCA CCTTTAGATA AAGAGAAAGG TGAAACACTG 
CTGAGTCCTT TGGTGATGTG TGGTCCCCAT GGCCTCAAGT TCCTGAAGCC TGTGGAGCTG 
CGCTTACCAC ACTGTGCGTC CATGACTCCT GACGGTTGGT CTTTTGCTCT AAAATCATCC 
GACTCCTCGT CGGGTGATCC TAAAACCTGG CAAAACAAGT GTCTTCCCGG AGATCCAAAT 
TATCTCGTTG GAGCAAACTG TGTTTCTGTC CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001330239.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317168.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA.

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CloneID	OHu48522
Clone ID Related Accession (Same CDS sequence)	NM_001330239.1 , NM_001330239.2 , NM_001330239.3
Accession Version	NM_001330239.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-23
Organism	Homo sapiens(Human)
Product	tight junction protein ZO-1 isoform e
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC022613.13. On Aug 20, 2016 this sequence version replaced XM_005254618.3. Transcript Variant: This variant (5) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (e) is longer than isoform a. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.266936.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317168.1
CDS	476..5782
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001330239.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330239.1

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001330239.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317168.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA.

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CloneID	OHu48522
Clone ID Related Accession (Same CDS sequence)	NM_001330239.1 , NM_001330239.2 , NM_001330239.3
Accession Version	NM_001330239.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022613.13, AB370197.1, CV571135.1, BE819398.1, DA261260.1 and AF035298.1. On Sep 1, 2017 this sequence version replaced NM_001330239.1. Transcript Variant: This variant (5) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (e) is longer than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.266936.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317168.1
CDS	476..5782
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001330239.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330239.2

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001330239.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317168.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA.

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CloneID	OHu48522
Clone ID Related Accession (Same CDS sequence)	NM_001330239.1 , NM_001330239.2 , NM_001330239.3
Accession Version	NM_001330239.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022613.13, AB370197.1, CV571135.1, BE819398.1, DA261260.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_001330239.2. Transcript Variant: This variant (5) uses an alternate in-frame splice site in the 3' coding region compared to variant 1. The encoded isoform (e) is longer than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.266936.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317168.1
CDS	441..5747
Translation	MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001330239.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330239.3

ORF Insert Sequence:

ATGTCCGCCA GAGCTGCGGC CGCCAAGAGC ACAGCAATGG AGGAAACAGC TATATGGGAA 
CAACATACAG TGACGCTTCA CAGGGCTCCT GGATTTGGAT TTGGAATTGC AATATCTGGT 
GGACGAGATA ATCCTCATTT TCAGAGTGGG GAAACGTCAA TAGTGATTTC AGATGTGCTG 
AAAGGAGGAC CAGCTGAAGG ACAGCTACAG GAAAATGACC GAGTTGCAAT GGTTAACGGA 
GTTTCAATGG ATAATGTTGA ACATGCTTTT GCTGTTCAGC AACTAAGGAA AAGTGGGAAA 
AATGCAAAAA TTACAATTAG AAGGAAGAAG AAAGTTCAAA TACCAGTAAG TCGTCCTGAT 
CCTGAACCAG TATCTGATAA TGAAGAAGAT AGTTATGATG AGGAAATACA TGATCCAAGA 
AGTGGCCGGA GTGGTGTGGT TAACAGAAGG AGTGAGAAGA TTTGGCCGAG GGATAGAAGT 
GCAAGTAGAG AGAGGAGCTT GTCCCCGCGG TCAGACAGGC GGTCAGTGGC TTCCAGCCAG 
CCTGCTAAAC CTACTAAAGT CACACTGGTG AAATCCCGGA AAAATGAAGA ATATGGTCTT 
CGATTGGCAA GCCATATATT TGTTAAGGAA ATTTCACAAG ATAGTTTGGC AGCAAGAGAT 
GGCAATATTC AAGAAGGTGA TGTTGTATTG AAGATAAATG GTACTGTGAC AGAAAATATG 
TCATTGACAG ATGCAAAGAC ATTGATAGAA AGGTCTAAAG GCAAATTAAA AATGGTAGTT 
CAAAGAGATG AACGGGCTAC GCTATTGAAT GTCCCTGATC TTTCTGACAG CATCCACTCT 
GCTAATGCCT CTGAGAGAGA CGACATTTCA GAAATTCAGT CACTGGCATC AGATCATTCT 
GGTCGATCAC ACGATAGGCC TCCCCGCCGC AGCCGGTCAC GATCTCCTGA CCAGCGGTCA 
GAGCCTTCTG ATCATTCCAG GCACTCGCCG CAGCAGCCAA GCAATGGCAG TCTCCGGAGT 
AGAGATGAAG AGAGAATTTC TAAACCTGGG GCTGTCTCAA CTCCTGTAAA GCATGCTGAT 
GATCACACAC CTAAAACAGT GGAAGAAGTT ACAGTTGAAA GAAATGAGAA ACAAACACCT 
TCTCTTCCAG AACCAAAGCC TGTGTATGCC CAAGTTGGGC AACCAGATGT GGATTTACCT 
GTCAGTCCAT CTGATGGTGT CCTACCTAAT TCAACTCATG AAGATGGGAT TCTTCGGCCC 
AGCATGAAAT TGGTAAAATT CAGAAAAGGA GATAGTGTGG GTTTGCGGCT GGCTGGTGGA 
AATGATGTTG GAATATTTGT AGCTGGCGTT CTAGAAGATA GCCCTGCAGC CAAGGAAGGC 
TTAGAGGAAG GTGATCAAAT TCTCAGGGTA AACAACGTAG ATTTTACAAA TATCATAAGA 
GAAGAAGCCG TCCTTTTCCT GCTTGACCTC CCTAAAGGAG AAGAAGTGAC CATATTGGCT 
CAGAAGAAGA AGGATGTTTA TCGTCGCATT GTAGAATCAG ATGTAGGAGA TTCTTTCTAT 
ATTAGAACCC ATTTTGAATA TGAAAAGGAA TCTCCCTATG GACTTAGTTT TAACAAAGGA 
GAGGTGTTCC GTGTTGTGGA TACCTTGTAC AATGGAAAAC TGGGCTCTTG GCTTGCTATT 
CGAATTGGTA AAAATCATAA GGAGGTAGAA CGAGGCATCA TCCCTAATAA GAACAGAGCT 
GAGCAGCTAG CCAGTGTACA GTATACACTT CCAAAAACAG CAGGCGGAGA CCGTGCTGAC 
TTCTGGAGAT TCAGAGGTCT TCGCAGCTCC AAGAGAAATC TTCGAAAAAG CAGAGAGGAT 
TTGTCCGCTC AGCCTGTTCA AACAAAGTTT CCAGCTTATG AAAGAGTGGT TCTTCGAGAA 
GCTGGATTTC TGAGGCCTGT AACCATTTTT GGACCAATAG CTGATGTTGC CAGAGAAAAG 
CTGGCAAGAG AAGAACCAGA TATTTATCAA ATTGCAAAGA GTGAACCACG AGACGCTGGA 
ACTGACCAAC GTAGCTCTGG CATTATTCGC CTGCATACAA TAAAGCAAAT CATAGATCAA 
GACAAACATG CTTTATTAGA TGTAACACCA AATGCAGTTG ATCGTCTTAA CTATGCCCAG 
TGGTATCCAA TTGTTGTATT TCTTAACCCT GATTCTAAGC AAGGAGTAAA AACAATGAGA 
ATGAGGTTAT GTCCAGAATC TCGGAAAAGT GCCAGGAAGT TATACGAGCG ATCTCATAAA 
CTTCGTAAAA ATAATCACCA TCTTTTTACA ACTACAATTA ACTTAAATTC AATGAATGAT 
GGTTGGTATG GTGCGCTGAA AGAAGCAATT CAACAACAGC AAAACCAGCT GGTATGGGTT 
TCCGAGGGAA AGGCGGATGG TGCTACAAGT GATGACCTTG ATTTGCATGA TGATCGTCTG 
TCCTACCTGT CAGCTCCAGG TAGTGAATAC TCAATGTATA GCACGGACAG TAGACACACT 
TCTGACTATG AAGACACAGA CACAGAAGGC GGGGCCTACA CTGATCAAGA ACTAGATGAA 
ACTCTTAATG ATGAGGTTGG GACTCCACCG GAGTCTGCCA TTACACGGTC CTCTGAGCCT 
GTAAGAGAGG ACTCCTCTGG AATGCATCAT GAAAACCAAA CATATCCTCC TTACTCACCA 
CAAGCGCAGC CACAACCAAT TCATAGAATA GACTCCCCTG GATTTAAGCC AGCCTCTCAA 
CAGAAAGCAG AAGCTTCATC TCCAGTCCCT TACCTTTCGC CTGAAACAAA CCCAGCATCA 
TCAACCTCTG CTGTTAATCA TAATGTAAAT TTAACTAATG TCAGACTGGA GGAGCCCACC 
CCAGCTCCTT CCACCTCTTA CTCACCACAA GCTGATTCTT TAAGAACACC AAGTACTGAG 
GCAGCTCACA TAATGCTAAG AGATCAAGAA CCATCATTGT CGTCGCATGT AGATCCAACA 
AAGGTGTATA GAAAGGATCC ATATCCCGAG GAAATGATGA GGCAGAACCA TGTTTTGAAA 
CAGCCAGCCG TTAGTCACCC AGGGCACAGG CCAGACAAAG AGCCTAATCT GACCTATGAA 
CCCCAACTCC CATACGTAGA GAAACAAGCC AGCAGAGACC TCGAGCAGCC CACATACAGA 
TACGAGTCCT CAAGCTATAC GGACCAGTTT TCTCGAAACT ATGAACATCG TCTGCGATAC 
GAAGATCGCG TCCCCATGTA TGAAGAACAG TGGTCATATT ATGATGACAA ACAGCCCTAC 
CCATCTCGGC CACCTTTTGA TAATCAGCAC TCTCAAGACC TTGACTCCAG ACAGCATCCC 
GAAGAGTCCT CAGAACGAGG GTACTTTCCA CGTTTTGAAG AGCCAGCCCC TCTGTCTTAC 
GACAGCAGAC CACGTTACGA ACAGGCACCT AGAGCATCCG CCCTGCGGCA CGAAGAGCAG 
CCAGCTCCTG GGTATGACAC ACATGGTAGA CTCAGACCGG AAGCCCAGCC CCACCCTTCA 
GCAGGGCCCA AGCCTGCAGA GTCCAAGCAG TATTTTGAGC AATATTCACG CAGTTACGAG 
CAAGTACCAC CCCAAGGATT TACCTCTAGA GCAGGTCATT TTGAGCCTCT CCATGGTGCT 
GCAGCTGTCC CTCCGCTGAT ACCTTCATCT CAGCATAAGC CAGAAGCTCT GCCTTCAAAC 
ACCAAACCAC TGCCTCCACC CCCAACTCAA ACCGAAGAAG AGGAAGATCC AGCAATGAAG 
CCACAGTCTG TACTCACCAG AGTTAAGATG TTTGAAAACA AAAGATCTGC ATCCTTAGAG 
ACCAAGAAGG ATGTAAATGA CACTGGCAGT TTTAAGCCTC CAGAAGTAGC ATCTAAACCT 
TCAGGTGCTC CCATCATTGG TCCCAAACCC ACTTCTCAGA ATCAATTCAG TGAACATGAC 
AAAACTCTGT ACAGGATCCC AGAACCTCAA AAACCTCAAC TGAAGCCACC TGAAGATATT 
GTTCGGTCCA ATCATTATGA CCCTGAAGAA GATGAAGAAT ATTATCGAAA ACAGCTGTCA 
TACTTTGACC GAAGAAGTTT TGAGAATAAG CCTCCTGCAC ACATTGCCGC CAGCCATCTC 
TCCGAGCCTG CAAAGCCAGC GCATTCTCAG AATCAATCAA ATTTTTCTAG TTATTCTTCA 
AAGGGAAAGC CTCCTGAAGC TGATGGTGTG GATAGATCAT TTGGCGAGAA ACGCTATGAA 
CCCATCCAGG CCACTCCCCC TCCTCCTCCA TTGCCCTCGC AGTATGCCCA GCCATCTCAG 
CCTGTCACCA GCGCGTCTCT CCACATACAT TCTAAGGGAG CACATGGTGA AGGTAATTCA 
GTGTCATTGG ATTTTCAGAA TTCCTTAGTG TCCAAACCAG ACCCACCTCC ATCTCAGAAT 
AAGCCAGCAA CTTTCAGACC ACCAAACCGA GAAGATACTG CTCAGGCAGC TTTCTATCCC 
CAGAAAAGTT TTCCAGATAA AGCCCCAGTT AATGGAACTG AACAGACTCA GAAAACAGTC 
ACTCCAGCAT ACAATCGATT CACACCAAAA CCATATACAA GTTCTGCCCG ACCATTTGAA 
CGCAAGTTTG AAAGTCCTAA ATTCAATCAC AATCTTCTGC CAAGTGAAAC TGCACATAAA 
CCTGACTTGT CTTCAAAAAC TCCCACTTCT CCAAAAACTC TTGTGAAATC GCACAGTTTG 
GCACAGCCTC CTGAGTTTGA CAGTGGAGTT GAAACTTTCT CTATCCATGC AGAGAAGCCT 
AAATATCAAA TAAATAATAT CAGCACAGTG CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT 
GTGGAAGAGG ATGAAGATGA AGATGGTCAT ACTGTGGTGG CCACAGCCCG AGGCATATTT 
AACAGCAATG GGGGCGTGCT GAGTTCCATA GAAACTGGTG TTAGTATAAT TATCCCTCAA 
GGAGCCATTC CCGAAGGAGT TGAGCAGGAA ATCTATTTCA AGGTCTGCCG GGACAACAGC 
ATCCTTCCAC CTTTAGATAA AGAGAAAGGT GAAACACTGC TGAGTCCTTT GGTGATGTGT 
GGTCCCCATG GCCTCAAGTT CCTGAAGCCT GTGGAGCTGC GCTTACCACA CTGTGCGTCC 
ATGACTCCTG ACGGTTGGTC TTTTGCTCTA AAATCATCCG ACTCCTCGTC GGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001301026.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287955.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 4, mRNA.

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CloneID	OHu48525
Clone ID Related Accession (Same CDS sequence)	NM_001301026.2 , NM_001301026.1
Accession Version	NM_001301026.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640879.1, AC022613.13, AB370197.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_001301026.1. Transcript Variant: This variant (4) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, and it lacks an alternate in-frame exon in the central coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX640879.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287955.1
CDS	174..5252
Translation	MERNTFSMDCHSKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001301026.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301026.2

ORF Insert Sequence:

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001301026.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287955.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 4, mRNA.

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CloneID	OHu48525
Clone ID Related Accession (Same CDS sequence)	NM_001301026.2 , NM_001301026.1
Accession Version	NM_001301026.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5079bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-12
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX640879.1, AC022613.13, AB370197.1 and AF035298.1. On or before Jul 18, 2014 this sequence version replaced XM_006725559.1, XM_006720661.1. Transcript Variant: This variant (4) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, and it lacks an alternate in-frame exon in the central coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (d) has a distinct N-terminus and is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX640879.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287955.1
CDS	243..5321
Translation	MERNTFSMDCHSKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001301026.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301026.1

ORF Insert Sequence:

ATGGAGAGAA ATACGTTTTC AATGGATTGC CACAGTAAGA GCACAGCAAT GGAGGAAACA 
GCTATATGGG AACAACATAC AGTGACGCTT CACAGGGCTC CTGGATTTGG ATTTGGAATT 
GCAATATCTG GTGGACGAGA TAATCCTCAT TTTCAGAGTG GGGAAACGTC AATAGTGATT 
TCAGATGTGC TGAAAGGAGG ACCAGCTGAA GGACAGCTAC AGGAAAATGA CCGAGTTGCA 
ATGGTTAACG GAGTTTCAAT GGATAATGTT GAACATGCTT TTGCTGTTCA GCAACTAAGG 
AAAAGTGGGA AAAATGCAAA AATTACAATT AGAAGGAAGA AGAAAGTTCA AATACCAGTA 
AGTCGTCCTG ATCCTGAACC AGTATCTGAT AATGAAGAAG ATAGTTATGA TGAGGAAATA 
CATGATCCAA GAAGTGGCCG GAGTGGTGTG GTTAACAGAA GGAGTGAGAA GATTTGGCCG 
AGGGATAGAA GTGCAAGTAG AGAGAGGAGC TTGTCCCCGC GGTCAGACAG GCGGTCAGTG 
GCTTCCAGCC AGCCTGCTAA ACCTACTAAA GTCACACTGG TGAAATCCCG GAAAAATGAA 
GAATATGGTC TTCGATTGGC AAGCCATATA TTTGTTAAGG AAATTTCACA AGATAGTTTG 
GCAGCAAGAG ATGGCAATAT TCAAGAAGGT GATGTTGTAT TGAAGATAAA TGGTACTGTG 
ACAGAAAATA TGTCATTGAC AGATGCAAAG ACATTGATAG AAAGGTCTAA AGGCAAATTA 
AAAATGGTAG TTCAAAGAGA TGAACGGGCT ACGCTATTGA ATGTCCCTGA TCTTTCTGAC 
AGCATCCACT CTGCTAATGC CTCTGAGAGA GACGACATTT CAGAAATTCA GTCACTGGCA 
TCAGATCATT CTGGTCGATC ACACGATAGG CCTCCCCGCC GCAGCCGGTC ACGATCTCCT 
GACCAGCGGT CAGAGCCTTC TGATCATTCC AGGCACTCGC CGCAGCAGCC AAGCAATGGC 
AGTCTCCGGA GTAGAGATGA AGAGAGAATT TCTAAACCTG GGGCTGTCTC AACTCCTGTA 
AAGCATGCTG ATGATCACAC ACCTAAAACA GTGGAAGAAG TTACAGTTGA AAGAAATGAG 
AAACAAACAC CTTCTCTTCC AGAACCAAAG CCTGTGTATG CCCAAGTTGG GCAACCAGAT 
GTGGATTTAC CTGTCAGTCC ATCTGATGGT GTCCTACCTA ATTCAACTCA TGAAGATGGG 
ATTCTTCGGC CCAGCATGAA ATTGGTAAAA TTCAGAAAAG GAGATAGTGT GGGTTTGCGG 
CTGGCTGGTG GAAATGATGT TGGAATATTT GTAGCTGGCG TTCTAGAAGA TAGCCCTGCA 
GCCAAGGAAG GCTTAGAGGA AGGTGATCAA ATTCTCAGGG TAAACAACGT AGATTTTACA 
AATATCATAA GAGAAGAAGC CGTCCTTTTC CTGCTTGACC TCCCTAAAGG AGAAGAAGTG 
ACCATATTGG CTCAGAAGAA GAAGGATGTT TATCGTCGCA TTGTAGAATC AGATGTAGGA 
GATTCTTTCT ATATTAGAAC CCATTTTGAA TATGAAAAGG AATCTCCCTA TGGACTTAGT 
TTTAACAAAG GAGAGGTGTT CCGTGTTGTG GATACCTTGT ACAATGGAAA ACTGGGCTCT 
TGGCTTGCTA TTCGAATTGG TAAAAATCAT AAGGAGGTAG AACGAGGCAT CATCCCTAAT 
AAGAACAGAG CTGAGCAGCT AGCCAGTGTA CAGTATACAC TTCCAAAAAC AGCAGGCGGA 
GACCGTGCTG ACTTCTGGAG ATTCAGAGGT CTTCGCAGCT CCAAGAGAAA TCTTCGAAAA 
AGCAGAGAGG ATTTGTCCGC TCAGCCTGTT CAAACAAAGT TTCCAGCTTA TGAAAGAGTG 
GTTCTTCGAG AAGCTGGATT TCTGAGGCCT GTAACCATTT TTGGACCAAT AGCTGATGTT 
GCCAGAGAAA AGCTGGCAAG AGAAGAACCA GATATTTATC AAATTGCAAA GAGTGAACCA 
CGAGACGCTG GAACTGACCA ACGTAGCTCT GGCATTATTC GCCTGCATAC AATAAAGCAA 
ATCATAGATC AAGACAAACA TGCTTTATTA GATGTAACAC CAAATGCAGT TGATCGTCTT 
AACTATGCCC AGTGGTATCC AATTGTTGTA TTTCTTAACC CTGATTCTAA GCAAGGAGTA 
AAAACAATGA GAATGAGGTT ATGTCCAGAA TCTCGGAAAA GTGCCAGGAA GTTATACGAG 
CGATCTCATA AACTTCGTAA AAATAATCAC CATCTTTTTA CAACTACAAT TAACTTAAAT 
TCAATGAATG ATGGTTGGTA TGGTGCGCTG AAAGAAGCAA TTCAACAACA GCAAAACCAG 
CTGGTATGGG TTTCCGAGGG AAAGGCGGAT GGTGCTACAA GTGATGACCT TGATTTGCAT 
GATGATCGTC TGTCCTACCT GTCAGCTCCA GGTAGTGAAT ACTCAATGTA TAGCACGGAC 
AGTAGACACA CTTCTGACTA TGAAGACACA GACACAGAAG GCGGGGCCTA CACTGATCAA 
GAACTAGATG AAACTCTTAA TGATGAGGTT GGGACTCCAC CGGAGTCTGC CATTACACGG 
TCCTCTGAGC CTGTAAGAGA GGACTCCTCT GGAATGCATC ATGAAAACCA AACATATCCT 
CCTTACTCAC CACAAGCGCA GCCACAACCA ATTCATAGAA TAGACTCCCC TGGATTTAAG 
CCAGCCTCTC AACAGGTGTA TAGAAAGGAT CCATATCCCG AGGAAATGAT GAGGCAGAAC 
CATGTTTTGA AACAGCCAGC CGTTAGTCAC CCAGGGCACA GGCCAGACAA AGAGCCTAAT 
CTGACCTATG AACCCCAACT CCCATACGTA GAGAAACAAG CCAGCAGAGA CCTCGAGCAG 
CCCACATACA GATACGAGTC CTCAAGCTAT ACGGACCAGT TTTCTCGAAA CTATGAACAT 
CGTCTGCGAT ACGAAGATCG CGTCCCCATG TATGAAGAAC AGTGGTCATA TTATGATGAC 
AAACAGCCCT ACCCATCTCG GCCACCTTTT GATAATCAGC ACTCTCAAGA CCTTGACTCC 
AGACAGCATC CCGAAGAGTC CTCAGAACGA GGGTACTTTC CACGTTTTGA AGAGCCAGCC 
CCTCTGTCTT ACGACAGCAG ACCACGTTAC GAACAGGCAC CTAGAGCATC CGCCCTGCGG 
CACGAAGAGC AGCCAGCTCC TGGGTATGAC ACACATGGTA GACTCAGACC GGAAGCCCAG 
CCCCACCCTT CAGCAGGGCC CAAGCCTGCA GAGTCCAAGC AGTATTTTGA GCAATATTCA 
CGCAGTTACG AGCAAGTACC ACCCCAAGGA TTTACCTCTA GAGCAGGTCA TTTTGAGCCT 
CTCCATGGTG CTGCAGCTGT CCCTCCGCTG ATACCTTCAT CTCAGCATAA GCCAGAAGCT 
CTGCCTTCAA ACACCAAACC ACTGCCTCCA CCCCCAACTC AAACCGAAGA AGAGGAAGAT 
CCAGCAATGA AGCCACAGTC TGTACTCACC AGAGTTAAGA TGTTTGAAAA CAAAAGATCT 
GCATCCTTAG AGACCAAGAA GGATGTAAAT GACACTGGCA GTTTTAAGCC TCCAGAAGTA 
GCATCTAAAC CTTCAGGTGC TCCCATCATT GGTCCCAAAC CCACTTCTCA GAATCAATTC 
AGTGAACATG ACAAAACTCT GTACAGGATC CCAGAACCTC AAAAACCTCA ACTGAAGCCA 
CCTGAAGATA TTGTTCGGTC CAATCATTAT GACCCTGAAG AAGATGAAGA ATATTATCGA 
AAACAGCTGT CATACTTTGA CCGAAGAAGT TTTGAGAATA AGCCTCCTGC ACACATTGCC 
GCCAGCCATC TCTCCGAGCC TGCAAAGCCA GCGCATTCTC AGAATCAATC AAATTTTTCT 
AGTTATTCTT CAAAGGGAAA GCCTCCTGAA GCTGATGGTG TGGATAGATC ATTTGGCGAG 
AAACGCTATG AACCCATCCA GGCCACTCCC CCTCCTCCTC CATTGCCCTC GCAGTATGCC 
CAGCCATCTC AGCCTGTCAC CAGCGCGTCT CTCCACATAC ATTCTAAGGG AGCACATGGT 
GAAGGTAATT CAGTGTCATT GGATTTTCAG AATTCCTTAG TGTCCAAACC AGACCCACCT 
CCATCTCAGA ATAAGCCAGC AACTTTCAGA CCACCAAACC GAGAAGATAC TGCTCAGGCA 
GCTTTCTATC CCCAGAAAAG TTTTCCAGAT AAAGCCCCAG TTAATGGAAC TGAACAGACT 
CAGAAAACAG TCACTCCAGC ATACAATCGA TTCACACCAA AACCATATAC AAGTTCTGCC 
CGACCATTTG AACGCAAGTT TGAAAGTCCT AAATTCAATC ACAATCTTCT GCCAAGTGAA 
ACTGCACATA AACCTGACTT GTCTTCAAAA ACTCCCACTT CTCCAAAAAC TCTTGTGAAA 
TCGCACAGTT TGGCACAGCC TCCTGAGTTT GACAGTGGAG TTGAAACTTT CTCTATCCAT 
GCAGAGAAGC CTAAATATCA AATAAATAAT ATCAGCACAG TGCCTAAAGC TATTCCTGTG 
AGTCCTTCAG CTGTGGAAGA GGATGAAGAT GAAGATGGTC ATACTGTGGT GGCCACAGCC 
CGAGGCATAT TTAACAGCAA TGGGGGCGTG CTGAGTTCCA TAGAAACTGG TGTTAGTATA 
ATTATCCCTC AAGGAGCCAT TCCCGAAGGA GTTGAGCAGG AAATCTATTT CAAGGTCTGC 
CGGGACAACA GCATCCTTCC ACCTTTAGAT AAAGAGAAAG GTGAAACACT GCTGAGTCCT 
TTGGTGATGT GTGGTCCCCA TGGCCTCAAG TTCCTGAAGC CTGTGGAGCT GCGCTTACCA 
CACTGTGCGT CCATGACTCC TGACGGTTGG TCTTTTGCTC TAAAATCATC CGACTCCTCG 
TCGGGTGATC CTAAAACCTG GCAAAACAAG TGTCTTCCCG GAGATCCAAA TTATCTCGTT 
GGAGCAAACT GTGTTTCTGT CCTTATTGAC CACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001301025.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287954.2 Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA.

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CloneID	OHu102941
Clone ID Related Accession (Same CDS sequence)	NM_001301025.3 , NM_001301025.2
Accession Version	NM_001301025.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087455.7, AC022613.13 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_001301025.2. Transcript Variant: This variant (3) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, and it uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is longer than isoform a. The exon combination of this variant is inferred based on partial transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.29109.1, SRR1803611.232016.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287954.2
CDS	211..5796
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001301025.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301025.3

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001301025.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001287954.2 Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA.

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CloneID	OHu102941
Clone ID Related Accession (Same CDS sequence)	NM_001301025.3 , NM_001301025.2
Accession Version	NM_001301025.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5586bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-12
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087455.7, AC022613.13 and AF035298.1. On Sep 1, 2017 this sequence version replaced NM_001301025.1. Transcript Variant: This variant (3) contains alternate 5' exon structure and it thus differs in the 5' UTR and 5' coding region, and it uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (c) has a distinct N-terminus and is longer than isoform a. The exon combination of this variant is inferred based on partial transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.29109.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001287954.2
CDS	86..5671
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001301025.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001301025.2

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001355012.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341941.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 6, mRNA.

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CloneID	OHu85630
Clone ID Related Accession (Same CDS sequence)	NM_001355012.1 , XM_005254617.3 , NM_001355012.2
Accession Version	NM_001355012.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-12
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087455.7, CN372462.1, CN372473.1, CD653673.1, BC111712.1, BQ434619.1, AL707462.1, BE082525.1, HY197781.1, AA101028.1, BX504167.1, DB089107.1, CV571135.1, BQ575449.1, AC022613.13, BE819398.1, BI914113.1, BM459712.1, BX640879.1 and AF035298.1. On Sep 1, 2017 this sequence version replaced XM_005254617.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.27168.1, SRR1803617.114641.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341941.1
CDS	86..5611
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001355012.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355012.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( NM_001355012.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001341941.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant 6, mRNA.

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CloneID	OHu85630
Clone ID Related Accession (Same CDS sequence)	NM_001355012.1 , XM_005254617.3 , NM_001355012.2
Accession Version	NM_001355012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC087455.7, CN372462.1, CN372473.1, CD653673.1, BC111712.1, BQ434619.1, AL707462.1, BE082525.1, HY197781.1, AA101028.1, BX504167.1, DB089107.1, CV571135.1, BQ575449.1, AC022613.13, BE819398.1, BI914113.1, BM459712.1, BX640879.1 and AF035298.1. On Jun 2, 2019 this sequence version replaced NM_001355012.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.27168.1, SRR1803617.114641.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001341941.1
CDS	211..5736
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	NM_001355012.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355012.2

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017022521.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878010.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant X1, mRNA.

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CloneID	OHu85626
Clone ID Related Accession (Same CDS sequence)	XM_017022521.1
Accession Version	XM_017022521.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5637bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT 
AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG 
GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA 
TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT 
TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA 
GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT 
AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA 
CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC 
AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT 
CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG 
CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT 
ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA 
GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA 
ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT 
GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTACCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA 
AAATCATCCG ACTCCTCGTC GGGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCCGGA 
GATCCAAATT ATCTCGTTGG AGCAAACTGT GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878010.1
CDS	81..5717
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKFLGSYTSYDYLKRNVKWGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017022521.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022521.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT 
AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG 
GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA 
TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT 
TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA 
GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT 
AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA 
CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC 
AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT 
CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG 
CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT 
ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA 
GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA 
ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT 
GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTACCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA 
AAATCATCCG ACTCCTCGTC GGGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCCGGA 
GATCCAAATT ATCTCGTTGG AGCAAACTGT GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017022522.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878011.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant X2, mRNA.

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CloneID	OHu85627
Clone ID Related Accession (Same CDS sequence)	XM_017022522.1
Accession Version	XM_017022522.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5598bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT 
AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG 
GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA 
TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT 
TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA 
GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT 
AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA 
CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC 
AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT 
CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG 
CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT 
ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA 
GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA 
ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT 
GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTACCACA CTGTGCGTCC ATGACTCCTG ACGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCCGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC 
CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878011.1
CDS	81..5678
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKFLGSYTSYDYLKRNVKWGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017022522.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022522.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT 
AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG 
GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA 
TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT 
TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA 
GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT 
AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA 
CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC 
AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT 
CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG 
CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT 
ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA 
GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA 
ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT 
GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTACCACA CTGTGCGTCC ATGACTCCTG ACGGTGATCC TAAAACCTGG 
CAAAACAAGT GTCTTCCCGG AGATCCAAAT TATCTCGTTG GAGCAAACTG TGTTTCTGTC 
CTTATTGACC ACTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017022523.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878012.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant X3, mRNA.

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CloneID	OHu85628
Clone ID Related Accession (Same CDS sequence)	XM_017022523.1
Accession Version	XM_017022523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5577bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT 
AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG 
GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA 
TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT 
TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA 
GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT 
AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA 
CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC 
AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT 
CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG 
CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT 
ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA 
GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA 
ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT 
GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTACCACA CTGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCCGGA 
GATCCAAATT ATCTCGTTGG AGCAAACTGT GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878012.1
CDS	81..5657
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKFLGSYTSYDYLKRNVKWGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017022523.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022523.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT 
AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG 
GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA 
TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT 
TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA 
GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT 
AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA 
CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC 
AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT 
CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG 
CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT 
ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA 
GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA 
ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT 
GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTACCACA CTGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCCGGA 
GATCCAAATT ATCTCGTTGG AGCAAACTGT GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_011521972.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520274.2 Homo sapiens tight junction protein 1 (TJP1), transcript variant X4, mRNA.

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CloneID	OHu85629
Clone ID Related Accession (Same CDS sequence)	XM_011521972.2
Accession Version	XM_011521972.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5547bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521972.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520274.2
CDS	81..5627
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_011521972.2
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521972.2

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTGATCCT 
AAAACCTGGC AAAACAAGTG TCTTCCCGGA GATCCAAATT ATCTCGTTGG AGCAAACTGT 
GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_005254617.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254674.2 Homo sapiens tight junction protein 1 (TJP1), transcript variant X5, mRNA.

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CloneID	OHu85630
Clone ID Related Accession (Same CDS sequence)	NM_001355012.1 , XM_005254617.3 , NM_001355012.2
Accession Version	XM_005254617.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5526bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(Human)
Product	tight junction protein ZO-1 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254617.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254674.2
CDS	81..5606
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_005254617.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254617.3

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGAAAGCAGA AGCTTCATCT 
CCAGTCCCTT ACCTTTCGCC TGAAACAAAC CCAGCATCAT CAACCTCTGC TGTTAATCAT 
AATGTAAATT TAACTAATGT CAGACTGGAG GAGCCCACCC CAGCTCCTTC CACCTCTTAC 
TCACCACAAG CTGATTCTTT AAGAACACCA AGTACTGAGG CAGCTCACAT AATGCTAAGA 
GATCAAGAAC CATCATTGTC GTCGCATGTA GATCCAACAA AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_017022524.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016878013.1 Homo sapiens tight junction protein 1 (TJP1), transcript variant X5, mRNA.

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CloneID	OHu85631
Clone ID Related Accession (Same CDS sequence)	XM_017022524.1
Accession Version	XM_017022524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5397bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT 
AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG 
GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA 
TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT 
TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA 
GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT 
AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA 
CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC 
AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT 
CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG 
CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT 
ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA 
GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA 
ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT 
GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTACCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA 
AAATCATCCG ACTCCTCGTC GGGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCCGGA 
GATCCAAATT ATCTCGTTGG AGCAAACTGT GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016878013.1
CDS	81..5477
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKFLGSYTSYDYLKRNVKWGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_017022524.1
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022524.1

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AATTTCTTGG CTCTTACACT 
AGCTATGACT ACTTGAAAAG GAACGTCAAG TGGGGAAAGC CTCCTGAAGC TGATGGTGTG 
GATAGATCAT TTGGCGAGAA ACGCTATGAA CCCATCCAGG CCACTCCCCC TCCTCCTCCA 
TTGCCCTCGC AGTATGCCCA GCCATCTCAG CCTGTCACCA GCGCGTCTCT CCACATACAT 
TCTAAGGGAG CACATGGTGA AGGTAATTCA GTGTCATTGG ATTTTCAGAA TTCCTTAGTG 
TCCAAACCAG ACCCACCTCC ATCTCAGAAT AAGCCAGCAA CTTTCAGACC ACCAAACCGA 
GAAGATACTG CTCAGGCAGC TTTCTATCCC CAGAAAAGTT TTCCAGATAA AGCCCCAGTT 
AATGGAACTG AACAGACTCA GAAAACAGTC ACTCCAGCAT ACAATCGATT CACACCAAAA 
CCATATACAA GTTCTGCCCG ACCATTTGAA CGCAAGTTTG AAAGTCCTAA ATTCAATCAC 
AATCTTCTGC CAAGTGAAAC TGCACATAAA CCTGACTTGT CTTCAAAAAC TCCCACTTCT 
CCAAAAACTC TTGTGAAATC GCACAGTTTG GCACAGCCTC CTGAGTTTGA CAGTGGAGTT 
GAAACTTTCT CTATCCATGC AGAGAAGCCT AAATATCAAA TAAATAATAT CAGCACAGTG 
CCTAAAGCTA TTCCTGTGAG TCCTTCAGCT GTGGAAGAGG ATGAAGATGA AGATGGTCAT 
ACTGTGGTGG CCACAGCCCG AGGCATATTT AACAGCAATG GGGGCGTGCT GAGTTCCATA 
GAAACTGGTG TTAGTATAAT TATCCCTCAA GGAGCCATTC CCGAAGGAGT TGAGCAGGAA 
ATCTATTTCA AGGTCTGCCG GGACAACAGC ATCCTTCCAC CTTTAGATAA AGAGAAAGGT 
GAAACACTGC TGAGTCCTTT GGTGATGTGT GGTCCCCATG GCCTCAAGTT CCTGAAGCCT 
GTGGAGCTGC GCTTACCACA CTGTGCGTCC ATGACTCCTG ACGGTTGGTC TTTTGCTCTA 
AAATCATCCG ACTCCTCGTC GGGTGATCCT AAAACCTGGC AAAACAAGTG TCTTCCCGGA 
GATCCAAATT ATCTCGTTGG AGCAAACTGT GTTTCTGTCC TTATTGACCA CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_005254619.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254676.2 Homo sapiens tight junction protein 1 (TJP1), transcript variant X9, mRNA.

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CloneID	OHu85635
Clone ID Related Accession (Same CDS sequence)	XM_005254619.3
Accession Version	XM_005254619.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254619.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254676.2
CDS	81..5426
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCASMTPDGWSFALKSSDSSSGDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_005254619.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254619.3

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGCGTCCA TGACTCCTGA CGGTTGGTCT 
TTTGCTCTAA AATCATCCGA CTCCTCGTCG GGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TJP1 ( XM_005254620.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005254677.2 Homo sapiens tight junction protein 1 (TJP1), transcript variant X10, mRNA.

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CloneID	OHu85636
Clone ID Related Accession (Same CDS sequence)	XM_005254620.3
Accession Version	XM_005254620.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5286bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	tight junction protein ZO-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005254620.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005254677.2
CDS	81..5366
Translation	MRRLEGLCLRRRRRRRGPARERSGDKMKYQKYLTVLQMAIGVTPSNRGSLLPLKRKLWVTPSSENPNGATSSVSQGKPSLRRIKGRLHRSKSLDSMDFCELTSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Target ORF information:

RefSeq Version	XM_005254620.3
Organism	Homo sapiens(human)
Definition	Homo sapiens tight junction protein 1 (TJP1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005254620.3

ORF Insert Sequence:

ATGCGGCGCC TGGAGGGGCT GTGTCTGCGG CGGCGGCGGA GGCGGCGGGG CCCTGCCCGC 
GAGCGGAGCG GGGACAAGAT GAAGTACCAG AAATACCTGA CGGTGCTGCA GATGGCCATC 
GGCGTCACCC CCTCCAACCG CGGCAGCCTC CTGCCGCTCA AGAGGAAGCT GTGGGTAACG 
CCATCCTCTG AAAATCCTAA TGGTGCTACT TCTAGTGTCA GCCAAGGAAA ACCCTCTTTA 
AGACGAATTA AAGGGAGATT ACACAGAAGC AAAAGCCTTG ATAGCATGGA TTTCTGTGAG 
CTCACTAGCA CAGCAATGGA GGAAACAGCT ATATGGGAAC AACATACAGT GACGCTTCAC 
AGGGCTCCTG GATTTGGATT TGGAATTGCA ATATCTGGTG GACGAGATAA TCCTCATTTT 
CAGAGTGGGG AAACGTCAAT AGTGATTTCA GATGTGCTGA AAGGAGGACC AGCTGAAGGA 
CAGCTACAGG AAAATGACCG AGTTGCAATG GTTAACGGAG TTTCAATGGA TAATGTTGAA 
CATGCTTTTG CTGTTCAGCA ACTAAGGAAA AGTGGGAAAA ATGCAAAAAT TACAATTAGA 
AGGAAGAAGA AAGTTCAAAT ACCAGTAAGT CGTCCTGATC CTGAACCAGT ATCTGATAAT 
GAAGAAGATA GTTATGATGA GGAAATACAT GATCCAAGAA GTGGCCGGAG TGGTGTGGTT 
AACAGAAGGA GTGAGAAGAT TTGGCCGAGG GATAGAAGTG CAAGTAGAGA GAGGAGCTTG 
TCCCCGCGGT CAGACAGGCG GTCAGTGGCT TCCAGCCAGC CTGCTAAACC TACTAAAGTC 
ACACTGGTGA AATCCCGGAA AAATGAAGAA TATGGTCTTC GATTGGCAAG CCATATATTT 
GTTAAGGAAA TTTCACAAGA TAGTTTGGCA GCAAGAGATG GCAATATTCA AGAAGGTGAT 
GTTGTATTGA AGATAAATGG TACTGTGACA GAAAATATGT CATTGACAGA TGCAAAGACA 
TTGATAGAAA GGTCTAAAGG CAAATTAAAA ATGGTAGTTC AAAGAGATGA ACGGGCTACG 
CTATTGAATG TCCCTGATCT TTCTGACAGC ATCCACTCTG CTAATGCCTC TGAGAGAGAC 
GACATTTCAG AAATTCAGTC ACTGGCATCA GATCATTCTG GTCGATCACA CGATAGGCCT 
CCCCGCCGCA GCCGGTCACG ATCTCCTGAC CAGCGGTCAG AGCCTTCTGA TCATTCCAGG 
CACTCGCCGC AGCAGCCAAG CAATGGCAGT CTCCGGAGTA GAGATGAAGA GAGAATTTCT 
AAACCTGGGG CTGTCTCAAC TCCTGTAAAG CATGCTGATG ATCACACACC TAAAACAGTG 
GAAGAAGTTA CAGTTGAAAG AAATGAGAAA CAAACACCTT CTCTTCCAGA ACCAAAGCCT 
GTGTATGCCC AAGTTGGGCA ACCAGATGTG GATTTACCTG TCAGTCCATC TGATGGTGTC 
CTACCTAATT CAACTCATGA AGATGGGATT CTTCGGCCCA GCATGAAATT GGTAAAATTC 
AGAAAAGGAG ATAGTGTGGG TTTGCGGCTG GCTGGTGGAA ATGATGTTGG AATATTTGTA 
GCTGGCGTTC TAGAAGATAG CCCTGCAGCC AAGGAAGGCT TAGAGGAAGG TGATCAAATT 
CTCAGGGTAA ACAACGTAGA TTTTACAAAT ATCATAAGAG AAGAAGCCGT CCTTTTCCTG 
CTTGACCTCC CTAAAGGAGA AGAAGTGACC ATATTGGCTC AGAAGAAGAA GGATGTTTAT 
CGTCGCATTG TAGAATCAGA TGTAGGAGAT TCTTTCTATA TTAGAACCCA TTTTGAATAT 
GAAAAGGAAT CTCCCTATGG ACTTAGTTTT AACAAAGGAG AGGTGTTCCG TGTTGTGGAT 
ACCTTGTACA ATGGAAAACT GGGCTCTTGG CTTGCTATTC GAATTGGTAA AAATCATAAG 
GAGGTAGAAC GAGGCATCAT CCCTAATAAG AACAGAGCTG AGCAGCTAGC CAGTGTACAG 
TATACACTTC CAAAAACAGC AGGCGGAGAC CGTGCTGACT TCTGGAGATT CAGAGGTCTT 
CGCAGCTCCA AGAGAAATCT TCGAAAAAGC AGAGAGGATT TGTCCGCTCA GCCTGTTCAA 
ACAAAGTTTC CAGCTTATGA AAGAGTGGTT CTTCGAGAAG CTGGATTTCT GAGGCCTGTA 
ACCATTTTTG GACCAATAGC TGATGTTGCC AGAGAAAAGC TGGCAAGAGA AGAACCAGAT 
ATTTATCAAA TTGCAAAGAG TGAACCACGA GACGCTGGAA CTGACCAACG TAGCTCTGGC 
ATTATTCGCC TGCATACAAT AAAGCAAATC ATAGATCAAG ACAAACATGC TTTATTAGAT 
GTAACACCAA ATGCAGTTGA TCGTCTTAAC TATGCCCAGT GGTATCCAAT TGTTGTATTT 
CTTAACCCTG ATTCTAAGCA AGGAGTAAAA ACAATGAGAA TGAGGTTATG TCCAGAATCT 
CGGAAAAGTG CCAGGAAGTT ATACGAGCGA TCTCATAAAC TTCGTAAAAA TAATCACCAT 
CTTTTTACAA CTACAATTAA CTTAAATTCA ATGAATGATG GTTGGTATGG TGCGCTGAAA 
GAAGCAATTC AACAACAGCA AAACCAGCTG GTATGGGTTT CCGAGGGAAA GGCGGATGGT 
GCTACAAGTG ATGACCTTGA TTTGCATGAT GATCGTCTGT CCTACCTGTC AGCTCCAGGT 
AGTGAATACT CAATGTATAG CACGGACAGT AGACACACTT CTGACTATGA AGACACAGAC 
ACAGAAGGCG GGGCCTACAC TGATCAAGAA CTAGATGAAA CTCTTAATGA TGAGGTTGGG 
ACTCCACCGG AGTCTGCCAT TACACGGTCC TCTGAGCCTG TAAGAGAGGA CTCCTCTGGA 
ATGCATCATG AAAACCAAAC ATATCCTCCT TACTCACCAC AAGCGCAGCC ACAACCAATT 
CATAGAATAG ACTCCCCTGG ATTTAAGCCA GCCTCTCAAC AGGTGTATAG AAAGGATCCA 
TATCCCGAGG AAATGATGAG GCAGAACCAT GTTTTGAAAC AGCCAGCCGT TAGTCACCCA 
GGGCACAGGC CAGACAAAGA GCCTAATCTG ACCTATGAAC CCCAACTCCC ATACGTAGAG 
AAACAAGCCA GCAGAGACCT CGAGCAGCCC ACATACAGAT ACGAGTCCTC AAGCTATACG 
GACCAGTTTT CTCGAAACTA TGAACATCGT CTGCGATACG AAGATCGCGT CCCCATGTAT 
GAAGAACAGT GGTCATATTA TGATGACAAA CAGCCCTACC CATCTCGGCC ACCTTTTGAT 
AATCAGCACT CTCAAGACCT TGACTCCAGA CAGCATCCCG AAGAGTCCTC AGAACGAGGG 
TACTTTCCAC GTTTTGAAGA GCCAGCCCCT CTGTCTTACG ACAGCAGACC ACGTTACGAA 
CAGGCACCTA GAGCATCCGC CCTGCGGCAC GAAGAGCAGC CAGCTCCTGG GTATGACACA 
CATGGTAGAC TCAGACCGGA AGCCCAGCCC CACCCTTCAG CAGGGCCCAA GCCTGCAGAG 
TCCAAGCAGT ATTTTGAGCA ATATTCACGC AGTTACGAGC AAGTACCACC CCAAGGATTT 
ACCTCTAGAG CAGGTCATTT TGAGCCTCTC CATGGTGCTG CAGCTGTCCC TCCGCTGATA 
CCTTCATCTC AGCATAAGCC AGAAGCTCTG CCTTCAAACA CCAAACCACT GCCTCCACCC 
CCAACTCAAA CCGAAGAAGA GGAAGATCCA GCAATGAAGC CACAGTCTGT ACTCACCAGA 
GTTAAGATGT TTGAAAACAA AAGATCTGCA TCCTTAGAGA CCAAGAAGGA TGTAAATGAC 
ACTGGCAGTT TTAAGCCTCC AGAAGTAGCA TCTAAACCTT CAGGTGCTCC CATCATTGGT 
CCCAAACCCA CTTCTCAGAA TCAATTCAGT GAACATGACA AAACTCTGTA CAGGATCCCA 
GAACCTCAAA AACCTCAACT GAAGCCACCT GAAGATATTG TTCGGTCCAA TCATTATGAC 
CCTGAAGAAG ATGAAGAATA TTATCGAAAA CAGCTGTCAT ACTTTGACCG AAGAAGTTTT 
GAGAATAAGC CTCCTGCACA CATTGCCGCC AGCCATCTCT CCGAGCCTGC AAAGCCAGCG 
CATTCTCAGA ATCAATCAAA TTTTTCTAGT TATTCTTCAA AGGGAAAGCC TCCTGAAGCT 
GATGGTGTGG ATAGATCATT TGGCGAGAAA CGCTATGAAC CCATCCAGGC CACTCCCCCT 
CCTCCTCCAT TGCCCTCGCA GTATGCCCAG CCATCTCAGC CTGTCACCAG CGCGTCTCTC 
CACATACATT CTAAGGGAGC ACATGGTGAA GGTAATTCAG TGTCATTGGA TTTTCAGAAT 
TCCTTAGTGT CCAAACCAGA CCCACCTCCA TCTCAGAATA AGCCAGCAAC TTTCAGACCA 
CCAAACCGAG AAGATACTGC TCAGGCAGCT TTCTATCCCC AGAAAAGTTT TCCAGATAAA 
GCCCCAGTTA ATGGAACTGA ACAGACTCAG AAAACAGTCA CTCCAGCATA CAATCGATTC 
ACACCAAAAC CATATACAAG TTCTGCCCGA CCATTTGAAC GCAAGTTTGA AAGTCCTAAA 
TTCAATCACA ATCTTCTGCC AAGTGAAACT GCACATAAAC CTGACTTGTC TTCAAAAACT 
CCCACTTCTC CAAAAACTCT TGTGAAATCG CACAGTTTGG CACAGCCTCC TGAGTTTGAC 
AGTGGAGTTG AAACTTTCTC TATCCATGCA GAGAAGCCTA AATATCAAAT AAATAATATC 
AGCACAGTGC CTAAAGCTAT TCCTGTGAGT CCTTCAGCTG TGGAAGAGGA TGAAGATGAA 
GATGGTCATA CTGTGGTGGC CACAGCCCGA GGCATATTTA ACAGCAATGG GGGCGTGCTG 
AGTTCCATAG AAACTGGTGT TAGTATAATT ATCCCTCAAG GAGCCATTCC CGAAGGAGTT 
GAGCAGGAAA TCTATTTCAA GGTCTGCCGG GACAACAGCA TCCTTCCACC TTTAGATAAA 
GAGAAAGGTG AAACACTGCT GAGTCCTTTG GTGATGTGTG GTCCCCATGG CCTCAAGTTC 
CTGAAGCCTG TGGAGCTGCG CTTACCACAC TGTGATCCTA AAACCTGGCA AAACAAGTGT 
CTTCCCGGAG ATCCAAATTA TCTCGTTGGA GCAAACTGTG TTTCTGTCCT TATTGACCAC 
TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TJP1
Entrez Gene ID	7082
Full Name	tight junction protein 1
Synonyms	ZO-1
General protein information	Preferred Names tight junction protein 1 Names tight junction protein ZO-1 zona occludens 1 zonula occludens 1 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q13.1
Summary	This gene encodes a member of the membrane-associated guanylate kinase (MAGUK) family of proteins, and acts as a tight junction adaptor protein that also regulates adherens junctions. Tight junctions regulate the movement of ions and macromolecules between endothelial and epithelial cells. The multidomain structure of this scaffold protein, including a postsynaptic density 95/disc-large/zona occludens (PDZ) domain, a Src homology (SH3) domain, a guanylate kinase (GuK) domain and unique (U) motifs all help to co-ordinate binding of transmembrane proteins, cytosolic proteins, and F-actin, which are required for tight junction function. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2017].
Disorder MIM:	601009

Homo sapiens (human)	TJP1	NP_003248.3
	TJP1	XP_002804738.1
Canis lupus familiaris (dog)	TJP1	NP_001003140.1
Rattus norvegicus (Norway rat)	Tjp1	NP_001099736.1
Danio rerio (zebrafish)	LOC565459	XP_003201509.2
Bos taurus (cattle)	TJP1	XP_002696696.3
Gallus gallus (chicken)	TJP1	XP_413773.4
Danio rerio (zebrafish)	tjp1a	XP_005168990.1
Pan troglodytes (chimpanzee)	TJP1	XP_001163120.1
Mus musculus (house mouse)	Tjp1	NP_033412.2
Xenopus tropicalis (tropical clawed frog)	tjp1	XP_004917990.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TJP1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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