TCOF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TCOF1
Entrez Gene ID	6949
Full Name	treacle ribosome biogenesis factor 1
Synonyms	MFD1,TCS,TCS1,treacle
General protein information	Preferred Names treacle ribosome biogenesis factor 1 Names treacle protein Treacher Collins syndrome protein Treacher Collins-Franceschetti syndrome 1 nucleolar trafficking phosphoprotein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q32-q33.1
Summary	This gene encodes a nucleolar protein with a LIS1 homology domain. The protein is involved in ribosomal DNA gene transcription through its interaction with upstream binding factor (UBF). Mutations in this gene have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2008].
Disorder MIM:	606847
Disorder Html:	Treacher Collins syndrome 1, 154500 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000356.4	NP_000347.2	treacle protein isoform b
XM_017009793.2	XP_016865282.1	treacle protein isoform X9
XM_005268509.4	XP_005268566.1	treacle protein isoform X12
NM_001135244.2	NP_001128716.1	treacle protein isoform e
NM_001008657.2	NP_001008657.1	treacle protein isoform c
XM_005268504.3	XP_005268561.1	treacle protein isoform X3
XM_011537678.2	XP_011535980.1	treacle protein isoform X7
NM_001135244.1	NP_001128716.1	treacle protein isoform e
NM_001371623.1	NP_001358552.1	treacle protein isoform h
NM_001008657.3	NP_001008657.1	treacle protein isoform c
NM_001135243.1	NP_001128715.1	treacle protein isoform d
XM_005268504.4	XP_005268561.1	treacle protein isoform X3
XM_017009794.2	XP_016865283.1	treacle protein isoform X10
XM_017009793.1	XP_016865282.1	treacle protein isoform X9
XM_005268502.3	XP_005268559.1	treacle protein isoform X1
XM_005268505.4	XP_005268562.1	treacle protein isoform X4
NM_001135245.1	NP_001128717.1	treacle protein isoform f
NM_001195141.1	NP_001182070.1	treacle protein isoform g
NM_001135245.2	NP_001128717.1	treacle protein isoform f
XM_005268502.4	XP_005268559.1	treacle protein isoform X1
XM_005268506.4	XP_005268563.1	treacle protein isoform X5
XM_011537678.3	XP_011535980.1	treacle protein isoform X7
XM_005268508.4	XP_005268565.1	treacle protein isoform X11
XM_017009795.2	XP_016865284.1	treacle protein isoform X13
XM_005268505.3	XP_005268562.1	treacle protein isoform X4
XM_005268506.3	XP_005268563.1	treacle protein isoform X5
XM_005268508.3	XP_005268565.1	treacle protein isoform X11
XM_017009794.1	XP_016865283.1	treacle protein isoform X10
XM_005268503.3	XP_005268560.1	treacle protein isoform X2
XM_005268503.4	XP_005268560.1	treacle protein isoform X2
XM_005268507.4	XP_005268564.1	treacle protein isoform X8
XM_017009792.1	XP_016865281.1	treacle protein isoform X6
XM_017009795.1	XP_016865284.1	treacle protein isoform X13
XM_005268509.3	XP_005268566.1	treacle protein isoform X12
XM_005268507.3	XP_005268564.1	treacle protein isoform X8
NM_000356.3	NP_000347.2	treacle protein isoform b
NM_001195141.2	NP_001182070.1	treacle protein isoform g
XM_017009792.2	XP_016865281.1	treacle protein isoform X6

Pathways from BioSystems

Homologies

Homo sapiens (human)	TCOF1	NP_001128717.1
Bos taurus (cattle)	TCOF1	XP_002689339.2
Rattus norvegicus (Norway rat)	Tcof1	NP_001099613.1
Canis lupus familiaris (dog)	TCOF1	NP_001003057.1
Pan troglodytes (chimpanzee)	TCOF1	XP_527074.4
Mus musculus (house mouse)	Tcof1	NP_001185913.1
	TCOF1	NP_001035041.1

Gene Ontology
Bibliography

Related articles in PubMed

Mutation screening of Chinese Treacher Collins syndrome patients identified novel TCOF1 mutations.
Chen Y, Guo L, Li CL, Shan J, Xu HS, Li JY, Sun S, Hao SJ, Jin L, Chai G, Zhang TY
Molecular genetics and genomics : MGG(2017 Dec)

Mutational Analysis of TCOF1, GSC, and HOXA2 in Patients With Treacher Collins Syndrome.
Hao S, Jin L, Wang H, Li C, Zheng F, Ma D, Zhang T
The Journal of craniofacial surgery27(6)e583-6(2016 Sep)

Tcof1-Related Molecular Networks in Treacher Collins Syndrome.
Dai J, Si J, Wang M, Huang L, Fang B, Shi J, Wang X, Shen G
The Journal of craniofacial surgery27(6)1420-6(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The analysis results showed that the Tcof1-related genes were enriched in various biological processes, including cell proliferation, apoptosis, cell cycle, differentiation, and migration.
Title: Tcof1-Related Molecular Networks in Treacher Collins Syndrome.

We report a clinical and extensive molecular study, including TCOF1, POLR1D, POLR1C, and EFTUD2 genes, in a series of 146 patients with TCS.
Title: Treacher Collins syndrome: a clinical and molecular study based on a large series of patients.

findings identify TCOF1 as a DDR factor that could cooperate with ATM and NBS1 to suppress inappropriate rDNA transcription and maintain genomic integrity after DNA damage.
Title: Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response.

Treacle-mediated NBS1 recruitment into the nucleoli regulates rRNA silencing in trans in the presence of distant chromosome breaks.
Title: The NBS1-Treacle complex controls ribosomal RNA transcription in response to DNA damage.

Mutations in TCOF1, POLR1C and POLR1D have all been implicated in causing TCS
Title: Treacher Collins Syndrome: the genetics of a craniofacial disease.

The following TCOF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TCOF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu39898	NM_001371623.1 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu28303	NM_001135243.1 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu28306	NM_001195141.2 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu28307	NM_000356.4 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu28308	NM_001135244.2 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu28310	NM_001135245.2 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu28312	NM_001008657.3 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu39895	XM_005268502.4 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu39896	XM_005268503.4 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu39897	XM_005268504.4 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu39898	XM_005268505.4 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu39899	XM_005268506.4 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu39900	XM_005268507.4 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu39901	XM_005268508.4 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu39902	XM_005268509.4 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu60933	XM_011537678.3 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu96032	XM_017009792.2 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu96033	XM_017009793.2 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu96034	XM_017009794.2 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu96035	XM_017009795.2 Latest version!	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
View Old Accession Versions >>
OHu28308	NM_001135244.1	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu28312	NM_001008657.2	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu39897	XM_005268504.3	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu96033	XM_017009793.1	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu28310	NM_001135245.1	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
OHu39895	XM_005268502.3	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu60933	XM_011537678.2	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu39898	XM_005268505.3	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu39899	XM_005268506.3	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu39901	XM_005268508.3	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu96034	XM_017009794.1	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu39896	XM_005268503.3	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$811.30 $1159.00
OHu96035	XM_017009795.1	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu39902	XM_005268509.3	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$475.30 $679.00
OHu39900	XM_005268507.3	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu28307	NM_000356.3	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu28306	NM_001195141.1	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
OHu96032	XM_017009792.1	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$727.30 $1039.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TCOF1 ( NM_001371623.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001358552.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 8, mRNA.

Price & Availability↑

CloneID	OHu39898
Clone ID Related Accession (Same CDS sequence)	NM_001371623.1 , XM_005268505.4 , XM_005268505.3
Accession Version	NM_001371623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	treacle protein isoform h
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011372.7. On Jul 19, 2019 this sequence version replaced XM_005268505.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001358552.1
CDS	51..4520
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	NM_001371623.1
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001371623.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( NM_001135243.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128715.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 4, mRNA.

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CloneID	OHu28303
Clone ID Related Accession (Same CDS sequence)	NM_001135243.1
Accession Version	NM_001135243.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-21
Organism	Homo sapiens(human)
Product	treacle protein isoform d
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC377932.1, AC011372.7, AY460334.1, BC016144.1, U76366.1 and BM664489.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (d). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AY460334.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGGTC CCACCCCCTC CAGGACAGAG 
ACCCTGGTGG AGGAGACCGC AGCAGAGTCC AGCGAGGATG ATGTGGTGGC GCCATCCCAG 
TCTCTCCTCT CAGGTTATAT GACCCCTGGA CTAACCCCAG CCAATTCCCA GGCCTCAAAA 
GCCACTCCCA AGCTAGACTC CAGCCCCTCA GTTTCCTCTA CTCTGGCCGC CAAAGATGAC 
CCAGATGGCA AGCAGGAGGC AAAGCCCCAA CAGGCAGCAG GCATGTTGTC CCCTAAAACA 
GGTGGAAAAG AGGCTGCTTC AGGCACCACA CCTCAGAAGT CCCGGAAGCC CAAGAAAGGG 
GCTGGGAACC CCCAAGCCTC AACCCTGGCG CTGCAAAGCA ACATCACCCA GTGCCTCCTG 
GGCCAACCCT GGCCCCTGAA TGAGGCCCAG GTGCAGGCCT CAGTGGTGAA GGTCCTGACT 
GAGCTGCTGG AACAGGAAAG AAAGAAGGTG GTGGACACCA CCAAGGAGAG CAGCAGGAAG 
GGCTGGGAGA GCCGCAAGCG GAAGCTATCG GGAGACCAGC CAGCTGCCAG GACCCCCAGG 
AGCAAGAAGA AGAAGAAGCT GGGGGCCGGG GAAGGTGGGG AGGCCTCTGT TTCCCCAGAA 
AAGACCTCCA CGACTTCCAA GGGGAAAGCA AAGAGAGACA AAGCAAGTGG TGATGTCAAG 
GAGAAGAAAG GGAAGGGGTC TCTTGGCTCC CAAGGGGCCA AGGACGAGCC AGAAGAGGAG 
CTTCAGAAGG GGATGGGGAC GGTTGAAGGT GGAGATCAAA GCAACCCAAA GAGCAAGAAG 
GAGAAGAAGA AATCCGACAA GAGAAAAAAA GACAAAGAAA AAAAAGAAAA GAAGAAGAAA 
GCAAAAAAGG CCTCAACCAA AGATTCTGAG TCACCGTCCC AGAAGAAAAA GAAGAAAAAG 
AAGAAGACAG CAGAGCAGAC TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128715.1
CDS	109..4575
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	NM_001135243.1
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135243.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGGTC CCACCCCCTC CAGGACAGAG 
ACCCTGGTGG AGGAGACCGC AGCAGAGTCC AGCGAGGATG ATGTGGTGGC GCCATCCCAG 
TCTCTCCTCT CAGGTTATAT GACCCCTGGA CTAACCCCAG CCAATTCCCA GGCCTCAAAA 
GCCACTCCCA AGCTAGACTC CAGCCCCTCA GTTTCCTCTA CTCTGGCCGC CAAAGATGAC 
CCAGATGGCA AGCAGGAGGC AAAGCCCCAA CAGGCAGCAG GCATGTTGTC CCCTAAAACA 
GGTGGAAAAG AGGCTGCTTC AGGCACCACA CCTCAGAAGT CCCGGAAGCC CAAGAAAGGG 
GCTGGGAACC CCCAAGCCTC AACCCTGGCG CTGCAAAGCA ACATCACCCA GTGCCTCCTG 
GGCCAACCCT GGCCCCTGAA TGAGGCCCAG GTGCAGGCCT CAGTGGTGAA GGTCCTGACT 
GAGCTGCTGG AACAGGAAAG AAAGAAGGTG GTGGACACCA CCAAGGAGAG CAGCAGGAAG 
GGCTGGGAGA GCCGCAAGCG GAAGCTATCG GGAGACCAGC CAGCTGCCAG GACCCCCAGG 
AGCAAGAAGA AGAAGAAGCT GGGGGCCGGG GAAGGTGGGG AGGCCTCTGT TTCCCCAGAA 
AAGACCTCCA CGACTTCCAA GGGGAAAGCA AAGAGAGACA AAGCAAGTGG TGATGTCAAG 
GAGAAGAAAG GGAAGGGGTC TCTTGGCTCC CAAGGGGCCA AGGACGAGCC AGAAGAGGAG 
CTTCAGAAGG GGATGGGGAC GGTTGAAGGT GGAGATCAAA GCAACCCAAA GAGCAAGAAG 
GAGAAGAAGA AATCCGACAA GAGAAAAAAA GACAAAGAAA AAAAAGAAAA GAAGAAGAAA 
GCAAAAAAGG CCTCAACCAA AGATTCTGAG TCACCGTCCC AGAAGAAAAA GAAGAAAAAG 
AAGAAGACAG CAGAGCAGAC TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( NM_001195141.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182070.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu28306
Clone ID Related Accession (Same CDS sequence)	NM_001195141.1 , NM_001195141.2
Accession Version	NM_001195141.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-20
Organism	Homo sapiens(human)
Product	treacle protein isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC377932.1, AC011372.7, BC016144.1, U76366.1 and BM664489.1. Transcript Variant: This variant (7) lacks an alternate in-frame exon in the 3' coding region, compared to variant 4. This results in a shorter protein (isoform g), compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAC CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGGTGAAG GGCCCCAGGG GGCCAAGTCA GCCCACACGC TGGGTCCCAC CCCCTCCAGG 
ACAGAGACCC TGGTGGAGGA GACCGCAGCA GAGTCCAGCG AGGATGATGT GGTGGCGCCA 
TCCCAGTCTC TCCTCTCAGG TTATATGACC CCTGGACTAA CCCCAGCCAA TTCCCAGGCC 
TCAAAAGCCA CTCCCAAGCT AGACTCCAGC CCCTCAGTTT CCTCTACTCT GGCCGCCAAA 
GATGACCCAG ATGGCAAGCA GGAGGCAAAG CCCCAACAGG CAGCAGGCAT GTTGTCCCCT 
AAAACAGGTG GAAAAGAGGC TGCTTCAGGC ACCACACCTC AGAAGTCCCG GAAGCCCAAG 
AAAGGGGCTG GGAACCCCCA AGCCTCAACC CTGGCGCTGC AAAGCAACAT CACCCAGTGC 
CTCCTGGGCC AACCCTGGCC CCTGAATGAG GCCCAGGTGC AGGCCTCAGT GGTGAAGGTC 
CTGACTGAGC TGCTGGAACA GGAAAGAAAG AAGGTGGTGG ACACCACCAA GGAGAGCAGC 
AGGAAGGGCT GGGAGAGCCG CAAGCGGAAG CTATCGGGAG ACCAGCCAGC TGCCAGGACC 
CCCAGGAGCA AGAAGAAGAA GAAGCTGGGG GCCGGGGAAG GTGGGGAGGC CTCTGTTTCC 
CCAGAAAAGA CCTCCACGAC TTCCAAGGGG AAAGCAAAGA GAGACAAAGC AAGTGGTGAT 
GTCAAGGAGA AGAAAGGGAA GGGGTCTCTT GGCTCCCAAG GGGCCAAGGA CGAGCCAGAA 
GAGGAGCTTC AGAAGGGGAT GGGGACGGTT GAAGGTGGAG ATCAAAGCAA CCCAAAGAGC 
AAGAAGGAGA AGAAGAAATC CGACAAGAGA AAAAAAGACA AAGAAAAAAA AGAAAAGAAG 
AAGAAAGCAA AAAAGGCCTC AACCAAAGAT TCTGAGTCAC CGTCCCAGAA GAAAAAGAAG 
AAAAAGAAGA AGACAGCAGA GCAGACTGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182070.1
CDS	109..4461
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	NM_001195141.1
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195141.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAC CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGGTGAAG GGCCCCAGGG GGCCAAGTCA GCCCACACGC TGGGTCCCAC CCCCTCCAGG 
ACAGAGACCC TGGTGGAGGA GACCGCAGCA GAGTCCAGCG AGGATGATGT GGTGGCGCCA 
TCCCAGTCTC TCCTCTCAGG TTATATGACC CCTGGACTAA CCCCAGCCAA TTCCCAGGCC 
TCAAAAGCCA CTCCCAAGCT AGACTCCAGC CCCTCAGTTT CCTCTACTCT GGCCGCCAAA 
GATGACCCAG ATGGCAAGCA GGAGGCAAAG CCCCAACAGG CAGCAGGCAT GTTGTCCCCT 
AAAACAGGTG GAAAAGAGGC TGCTTCAGGC ACCACACCTC AGAAGTCCCG GAAGCCCAAG 
AAAGGGGCTG GGAACCCCCA AGCCTCAACC CTGGCGCTGC AAAGCAACAT CACCCAGTGC 
CTCCTGGGCC AACCCTGGCC CCTGAATGAG GCCCAGGTGC AGGCCTCAGT GGTGAAGGTC 
CTGACTGAGC TGCTGGAACA GGAAAGAAAG AAGGTGGTGG ACACCACCAA GGAGAGCAGC 
AGGAAGGGCT GGGAGAGCCG CAAGCGGAAG CTATCGGGAG ACCAGCCAGC TGCCAGGACC 
CCCAGGAGCA AGAAGAAGAA GAAGCTGGGG GCCGGGGAAG GTGGGGAGGC CTCTGTTTCC 
CCAGAAAAGA CCTCCACGAC TTCCAAGGGG AAAGCAAAGA GAGACAAAGC AAGTGGTGAT 
GTCAAGGAGA AGAAAGGGAA GGGGTCTCTT GGCTCCCAAG GGGCCAAGGA CGAGCCAGAA 
GAGGAGCTTC AGAAGGGGAT GGGGACGGTT GAAGGTGGAG ATCAAAGCAA CCCAAAGAGC 
AAGAAGGAGA AGAAGAAATC CGACAAGAGA AAAAAAGACA AAGAAAAAAA AGAAAAGAAG 
AAGAAAGCAA AAAAGGCCTC AACCAAAGAT TCTGAGTCAC CGTCCCAGAA GAAAAAGAAG 
AAAAAGAAGA AGACAGCAGA GCAGACTGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( NM_001195141.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001182070.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu28306
Clone ID Related Accession (Same CDS sequence)	NM_001195141.1 , NM_001195141.2
Accession Version	NM_001195141.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4353bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	treacle protein isoform g
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011372.7, BC016144.1, U76366.1 and BM664489.1. On Jun 2, 2019 this sequence version replaced NM_001195141.1. Transcript Variant: This variant (7) lacks an alternate in-frame exon in the 3' coding region, compared to variant 4. This results in a shorter protein (isoform g), compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAC CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGGTGAAG GGCCCCAGGG GGCCAAGTCA GCCCACACGC TGGGTCCCAC CCCCTCCAGG 
ACAGAGACCC TGGTGGAGGA GACCGCAGCA GAGTCCAGCG AGGATGATGT GGTGGCGCCA 
TCCCAGTCTC TCCTCTCAGG TTATATGACC CCTGGACTAA CCCCAGCCAA TTCCCAGGCC 
TCAAAAGCCA CTCCCAAGCT AGACTCCAGC CCCTCAGTTT CCTCTACTCT GGCCGCCAAA 
GATGACCCAG ATGGCAAGCA GGAGGCAAAG CCCCAACAGG CAGCAGGCAT GTTGTCCCCT 
AAAACAGGTG GAAAAGAGGC TGCTTCAGGC ACCACACCTC AGAAGTCCCG GAAGCCCAAG 
AAAGGGGCTG GGAACCCCCA AGCCTCAACC CTGGCGCTGC AAAGCAACAT CACCCAGTGC 
CTCCTGGGCC AACCCTGGCC CCTGAATGAG GCCCAGGTGC AGGCCTCAGT GGTGAAGGTC 
CTGACTGAGC TGCTGGAACA GGAAAGAAAG AAGGTGGTGG ACACCACCAA GGAGAGCAGC 
AGGAAGGGCT GGGAGAGCCG CAAGCGGAAG CTATCGGGAG ACCAGCCAGC TGCCAGGACC 
CCCAGGAGCA AGAAGAAGAA GAAGCTGGGG GCCGGGGAAG GTGGGGAGGC CTCTGTTTCC 
CCAGAAAAGA CCTCCACGAC TTCCAAGGGG AAAGCAAAGA GAGACAAAGC AAGTGGTGAT 
GTCAAGGAGA AGAAAGGGAA GGGGTCTCTT GGCTCCCAAG GGGCCAAGGA CGAGCCAGAA 
GAGGAGCTTC AGAAGGGGAT GGGGACGGTT GAAGGTGGAG ATCAAAGCAA CCCAAAGAGC 
AAGAAGGAGA AGAAGAAATC CGACAAGAGA AAAAAAGACA AAGAAAAAAA AGAAAAGAAG 
AAGAAAGCAA AAAAGGCCTC AACCAAAGAT TCTGAGTCAC CGTCCCAGAA GAAAAAGAAG 
AAAAAGAAGA AGACAGCAGA GCAGACTGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001182070.1
CDS	51..4403
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	NM_001195141.2
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001195141.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAC CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGGTGAAG GGCCCCAGGG GGCCAAGTCA GCCCACACGC TGGGTCCCAC CCCCTCCAGG 
ACAGAGACCC TGGTGGAGGA GACCGCAGCA GAGTCCAGCG AGGATGATGT GGTGGCGCCA 
TCCCAGTCTC TCCTCTCAGG TTATATGACC CCTGGACTAA CCCCAGCCAA TTCCCAGGCC 
TCAAAAGCCA CTCCCAAGCT AGACTCCAGC CCCTCAGTTT CCTCTACTCT GGCCGCCAAA 
GATGACCCAG ATGGCAAGCA GGAGGCAAAG CCCCAACAGG CAGCAGGCAT GTTGTCCCCT 
AAAACAGGTG GAAAAGAGGC TGCTTCAGGC ACCACACCTC AGAAGTCCCG GAAGCCCAAG 
AAAGGGGCTG GGAACCCCCA AGCCTCAACC CTGGCGCTGC AAAGCAACAT CACCCAGTGC 
CTCCTGGGCC AACCCTGGCC CCTGAATGAG GCCCAGGTGC AGGCCTCAGT GGTGAAGGTC 
CTGACTGAGC TGCTGGAACA GGAAAGAAAG AAGGTGGTGG ACACCACCAA GGAGAGCAGC 
AGGAAGGGCT GGGAGAGCCG CAAGCGGAAG CTATCGGGAG ACCAGCCAGC TGCCAGGACC 
CCCAGGAGCA AGAAGAAGAA GAAGCTGGGG GCCGGGGAAG GTGGGGAGGC CTCTGTTTCC 
CCAGAAAAGA CCTCCACGAC TTCCAAGGGG AAAGCAAAGA GAGACAAAGC AAGTGGTGAT 
GTCAAGGAGA AGAAAGGGAA GGGGTCTCTT GGCTCCCAAG GGGCCAAGGA CGAGCCAGAA 
GAGGAGCTTC AGAAGGGGAT GGGGACGGTT GAAGGTGGAG ATCAAAGCAA CCCAAAGAGC 
AAGAAGGAGA AGAAGAAATC CGACAAGAGA AAAAAAGACA AAGAAAAAAA AGAAAAGAAG 
AAGAAAGCAA AAAAGGCCTC AACCAAAGAT TCTGAGTCAC CGTCCCAGAA GAAAAAGAAG 
AAAAAGAAGA AGACAGCAGA GCAGACTGTA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( NM_000356.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000347.2 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 2, mRNA.

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CloneID	OHu28307
Clone ID Related Accession (Same CDS sequence)	NM_000356.4 , NM_000356.3
Accession Version	NM_000356.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	treacle protein isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from U76366.1, BC016144.1 and BM664489.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_000356.3. Transcript Variant: This variant (2) lacks the alternate in-frame exon 7, also known as VIa, in the 5' coding region, compared to variant 4. The resulting isoform (b) lacks an internal segment but has the same N- and C-termini, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U40847.1, U76366.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGGT GATTAAACCC 
CCTCTGATTT TTGTCGACCC TAATCGTAGT CCAGCTGGCC CAGCTGCTAC ACCCGCACAA 
GCCCAGGCTG CAAGCACCCC GAGGAAGGCC CGAGCCTCGG AGAGCACAGC CAGGAGCTCC 
TCCTCCGAGA GCGAGGATGA GGACGTGATC CCCGCTACAC AGTGCTTGAC TCCTGGCATC 
AGAACCAATG TGGTGACCAT GCCCACTGCC CACCCAAGAA TAGCCCCCAA AGCCAGCATG 
GCTGGGGCCA GCAGCAGCAA GGAGTCCAGT CGGATATCAG ATGGCAAGAA ACAGGAGGGA 
CCAGCCACTC AGGTGTCAAA GAAGAACCCA GCTTCCCTCC CACTGACCCA GGCTGCCCTG 
AAGGTCCTCG CCCAGAAAGC CAGTGAGGCT CAGCCTCCTG TTGCCAGGAC CCAGCCTTCA 
AGTGGGGTTG ACAGTGCTGT GGGAACACTC CCTGCAACAA GTCCCCAGAG CACCTCCGTC 
CAGGCCAAAG GGACCAACAA GCTCAGAAAA CCTAAGCTTC CTGAGGTCCA GCAGGCCACC 
AAAGCCCCTG AGAGCTCAGA TGACAGTGAG GACAGCAGCG ACAGTTCTTC AGGGAGTGAG 
GAAGATGGTG AAGGGCCCCA GGGGGCCAAG TCAGCCCACA CGCTGGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCACCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGG AAGCTATCGG GAGACCAGCC AGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCGGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCAAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGACG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000347.2
CDS	51..4286
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	NM_000356.4
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000356.4

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGGT GATTAAACCC 
CCTCTGATTT TTGTCGACCC TAATCGTAGT CCAGCTGGCC CAGCTGCTAC ACCCGCACAA 
GCCCAGGCTG CAAGCACCCC GAGGAAGGCC CGAGCCTCGG AGAGCACAGC CAGGAGCTCC 
TCCTCCGAGA GCGAGGATGA GGACGTGATC CCCGCTACAC AGTGCTTGAC TCCTGGCATC 
AGAACCAATG TGGTGACCAT GCCCACTGCC CACCCAAGAA TAGCCCCCAA AGCCAGCATG 
GCTGGGGCCA GCAGCAGCAA GGAGTCCAGT CGGATATCAG ATGGCAAGAA ACAGGAGGGA 
CCAGCCACTC AGGTGTCAAA GAAGAACCCA GCTTCCCTCC CACTGACCCA GGCTGCCCTG 
AAGGTCCTCG CCCAGAAAGC CAGTGAGGCT CAGCCTCCTG TTGCCAGGAC CCAGCCTTCA 
AGTGGGGTTG ACAGTGCTGT GGGAACACTC CCTGCAACAA GTCCCCAGAG CACCTCCGTC 
CAGGCCAAAG GGACCAACAA GCTCAGAAAA CCTAAGCTTC CTGAGGTCCA GCAGGCCACC 
AAAGCCCCTG AGAGCTCAGA TGACAGTGAG GACAGCAGCG ACAGTTCTTC AGGGAGTGAG 
GAAGATGGTG AAGGGCCCCA GGGGGCCAAG TCAGCCCACA CGCTGGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCACCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGG AAGCTATCGG GAGACCAGCC AGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCGGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCAAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGACG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( NM_000356.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000347.2 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 2, mRNA.

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CloneID	OHu28307
Clone ID Related Accession (Same CDS sequence)	NM_000356.4 , NM_000356.3
Accession Version	NM_000356.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4236bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-19
Organism	Homo sapiens(human)
Product	treacle protein isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC377932.1, AC011372.7, U76366.1, BC016144.1 and BM664489.1. This sequence is a reference standard in the RefSeqGene project. On Sep 30, 2008 this sequence version replaced NM_000356.2. Transcript Variant: This variant (2) lacks the alternate in-frame exon 7, also known as VIa, in the 5' coding region, compared to variant 4. The resulting isoform (b) lacks an internal segment but has the same N- and C-termini, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U76366.1, U40847.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGGT GATTAAACCC 
CCTCTGATTT TTGTCGACCC TAATCGTAGT CCAGCTGGCC CAGCTGCTAC ACCCGCACAA 
GCCCAGGCTG CAAGCACCCC GAGGAAGGCC CGAGCCTCGG AGAGCACAGC CAGGAGCTCC 
TCCTCCGAGA GCGAGGATGA GGACGTGATC CCCGCTACAC AGTGCTTGAC TCCTGGCATC 
AGAACCAATG TGGTGACCAT GCCCACTGCC CACCCAAGAA TAGCCCCCAA AGCCAGCATG 
GCTGGGGCCA GCAGCAGCAA GGAGTCCAGT CGGATATCAG ATGGCAAGAA ACAGGAGGGA 
CCAGCCACTC AGGTGTCAAA GAAGAACCCA GCTTCCCTCC CACTGACCCA GGCTGCCCTG 
AAGGTCCTCG CCCAGAAAGC CAGTGAGGCT CAGCCTCCTG TTGCCAGGAC CCAGCCTTCA 
AGTGGGGTTG ACAGTGCTGT GGGAACACTC CCTGCAACAA GTCCCCAGAG CACCTCCGTC 
CAGGCCAAAG GGACCAACAA GCTCAGAAAA CCTAAGCTTC CTGAGGTCCA GCAGGCCACC 
AAAGCCCCTG AGAGCTCAGA TGACAGTGAG GACAGCAGCG ACAGTTCTTC AGGGAGTGAG 
GAAGATGGTG AAGGGCCCCA GGGGGCCAAG TCAGCCCACA CGCTGGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCACCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGG AAGCTATCGG GAGACCAGCC AGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCGGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCAAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGACG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000347.2
CDS	109..4344
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	NM_000356.3
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000356.3

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGGT GATTAAACCC 
CCTCTGATTT TTGTCGACCC TAATCGTAGT CCAGCTGGCC CAGCTGCTAC ACCCGCACAA 
GCCCAGGCTG CAAGCACCCC GAGGAAGGCC CGAGCCTCGG AGAGCACAGC CAGGAGCTCC 
TCCTCCGAGA GCGAGGATGA GGACGTGATC CCCGCTACAC AGTGCTTGAC TCCTGGCATC 
AGAACCAATG TGGTGACCAT GCCCACTGCC CACCCAAGAA TAGCCCCCAA AGCCAGCATG 
GCTGGGGCCA GCAGCAGCAA GGAGTCCAGT CGGATATCAG ATGGCAAGAA ACAGGAGGGA 
CCAGCCACTC AGGTGTCAAA GAAGAACCCA GCTTCCCTCC CACTGACCCA GGCTGCCCTG 
AAGGTCCTCG CCCAGAAAGC CAGTGAGGCT CAGCCTCCTG TTGCCAGGAC CCAGCCTTCA 
AGTGGGGTTG ACAGTGCTGT GGGAACACTC CCTGCAACAA GTCCCCAGAG CACCTCCGTC 
CAGGCCAAAG GGACCAACAA GCTCAGAAAA CCTAAGCTTC CTGAGGTCCA GCAGGCCACC 
AAAGCCCCTG AGAGCTCAGA TGACAGTGAG GACAGCAGCG ACAGTTCTTC AGGGAGTGAG 
GAAGATGGTG AAGGGCCCCA GGGGGCCAAG TCAGCCCACA CGCTGGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCACCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGG AAGCTATCGG GAGACCAGCC AGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCGGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCAAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGACG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( NM_001135244.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128716.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu28308
Clone ID Related Accession (Same CDS sequence)	NM_001135244.2 , NM_001135244.1
Accession Version	NM_001135244.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	treacle protein isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011372.7, AK303611.1, BC016144.1, U76366.1 and BM664489.1. On Jun 2, 2019 this sequence version replaced NM_001135244.1. Transcript Variant: This variant (5) lacks the alternate in-frame exon 21, also known as XIX, and uses an alternate in-frame splice site in the central coding region, compared to variant 4. The resulting isoform (e) lacks an internal segment, contains a one residue insertion, and has the same N- and C-termini, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAC CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGGTGAAG GGCCCCAGGG GGCCAAGTCA GCCCACACGC TGGTAGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCACCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGG AAGCTATCGG GAGACCAGCC AGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCGGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCAAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGACG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128716.1
CDS	51..4406
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	NM_001135244.2
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135244.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAC CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGGTGAAG GGCCCCAGGG GGCCAAGTCA GCCCACACGC TGGTAGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCACCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGG AAGCTATCGG GAGACCAGCC AGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCGGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCAAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGACG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( NM_001135244.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128716.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu28308
Clone ID Related Accession (Same CDS sequence)	NM_001135244.2 , NM_001135244.1
Accession Version	NM_001135244.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4356bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-19
Organism	Homo sapiens(human)
Product	treacle protein isoform e
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC377932.1, AC011372.7, AK303611.1, BC016144.1, U76366.1 and BM664489.1. Transcript Variant: This variant (5) lacks the alternate in-frame exon 21, also known as XIX, and uses an alternate in-frame splice site in the central coding region, compared to variant 4. The resulting isoform (e) lacks an internal segment, contains a one residue insertion, and has the same N- and C-termini, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAC CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGGTGAAG GGCCCCAGGG GGCCAAGTCA GCCCACACGC TGGTAGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCACCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGG AAGCTATCGG GAGACCAGCC AGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCGGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCAAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGACG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128716.1
CDS	109..4464
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	NM_001135244.1
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135244.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAC CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGGTGAAG GGCCCCAGGG GGCCAAGTCA GCCCACACGC TGGTAGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCACCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGG AAGCTATCGG GAGACCAGCC AGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCGGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCAAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGACG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( NM_001135245.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128717.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 6, mRNA.

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CloneID	OHu28310
Clone ID Related Accession (Same CDS sequence)	NM_001135245.1 , NM_001135245.2
Accession Version	NM_001135245.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-10-19
Organism	Homo sapiens(human)
Product	treacle protein isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC377932.1, AC011372.7, BC027252.1, AK299167.1, U76366.1 and BM664489.1. Transcript Variant: This variant (6) lacks the alternate in-frame exon 7, also known as VIa, in the 5' coding region and uses an alternate in-frame splice site in the central coding region, compared to variant 4. The resulting isoform (f) lacks an internal segment, contains a one residue insertion, and has the same N- and C-termini, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGGT GATTAAACCC 
CCTCTGATTT TTGTCGACCC TAATCGTAGT CCAGCTGGCC CAGCTGCTAC ACCCGCACAA 
GCCCAGGCTG CAAGCACCCC GAGGAAGGCC CGAGCCTCGG AGAGCACAGC CAGGAGCTCC 
TCCTCCGAGA GCGAGGATGA GGACGTGATC CCCGCTACAC AGTGCTTGAC TCCTGGCATC 
AGAACCAATG TGGTGACCAT GCCCACTGCC CACCCAAGAA TAGCCCCCAA AGCCAGCATG 
GCTGGGGCCA GCAGCAGCAA GGAGTCCAGT CGGATATCAG ATGGCAAGAA ACAGGAGGGA 
CCAGCCACTC AGGTGTCAAA GAAGAACCCA GCTTCCCTCC CACTGACCCA GGCTGCCCTG 
AAGGTCCTCG CCCAGAAAGC CAGTGAGGCT CAGCCTCCTG TTGCCAGGAC CCAGCCTTCA 
AGTGGGGTTG ACAGTGCTGT GGGAACACTC CCTGCAACAA GTCCCCAGAG CACCTCCGTC 
CAGGCCAAAG GGACCAACAA GCTCAGAAAA CCTAAGCTTC CTGAGGTCCA GCAGGCCACC 
AAAGCCCCTG AGAGCTCAGA TGACAGTGAG GACAGCAGCG ACAGTTCTTC AGGGAGTGAG 
GAAGATGGTG AAGGGCCCCA GGGGGCCAAG TCAGCCCACA CGCTGGTAGG TCCCACCCCC 
TCCAGGACAG AGACCCTGGT GGAGGAGACC GCAGCAGAGT CCAGCGAGGA TGATGTGGTG 
GCGCCATCCC AGTCTCTCCT CTCAGGTTAT ATGACCCCTG GACTAACCCC AGCCAATTCC 
CAGGCCTCAA AAGCCACTCC CAAGCTAGAC TCCAGCCCCT CAGTTTCCTC TACTCTGGCC 
GCCAAAGATG ACCCAGATGG CAAGCAGGAG GCAAAGCCCC AACAGGCAGC AGGCATGTTG 
TCCCCTAAAA CAGGTGGAAA AGAGGCTGCT TCAGGCACCA CACCTCAGAA GTCCCGGAAG 
CCCAAGAAAG GGGCTGGGAA CCCCCAAGCC TCAACCCTGG CGCTGCAAAG CAACATCACC 
CAGTGCCTCC TGGGCCAACC CTGGCCCCTG AATGAGGCCC AGGTGCAGGC CTCAGTGGTG 
AAGGTCCTGA CTGAGCTGCT GGAACAGGAA AGAAAGAAGG TGGTGGACAC CACCAAGGAG 
AGCAGCAGGA AGGGCTGGGA GAGCCGCAAG CGGAAGCTAT CGGGAGACCA GCCAGCTGCC 
AGGACCCCCA GGAGCAAGAA GAAGAAGAAG CTGGGGGCCG GGGAAGGTGG GGAGGCCTCT 
GTTTCCCCAG AAAAGACCTC CACGACTTCC AAGGGGAAAG CAAAGAGAGA CAAAGCAAGT 
GGTGATGTCA AGGAGAAGAA AGGGAAGGGG TCTCTTGGCT CCCAAGGGGC CAAGGACGAG 
CCAGAAGAGG AGCTTCAGAA GGGGATGGGG ACGGTTGAAG GTGGAGATCA AAGCAACCCA 
AAGAGCAAGA AGGAGAAGAA GAAATCCGAC AAGAGAAAAA AAGACAAAGA AAAAAAAGAA 
AAGAAGAAGA AAGCAAAAAA GGCCTCAACC AAAGATTCTG AGTCACCGTC CCAGAAGAAA 
AAGAAGAAAA AGAAGAAGAC AGCAGAGCAG ACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128717.1
CDS	109..4347
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	NM_001135245.1
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135245.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGGT GATTAAACCC 
CCTCTGATTT TTGTCGACCC TAATCGTAGT CCAGCTGGCC CAGCTGCTAC ACCCGCACAA 
GCCCAGGCTG CAAGCACCCC GAGGAAGGCC CGAGCCTCGG AGAGCACAGC CAGGAGCTCC 
TCCTCCGAGA GCGAGGATGA GGACGTGATC CCCGCTACAC AGTGCTTGAC TCCTGGCATC 
AGAACCAATG TGGTGACCAT GCCCACTGCC CACCCAAGAA TAGCCCCCAA AGCCAGCATG 
GCTGGGGCCA GCAGCAGCAA GGAGTCCAGT CGGATATCAG ATGGCAAGAA ACAGGAGGGA 
CCAGCCACTC AGGTGTCAAA GAAGAACCCA GCTTCCCTCC CACTGACCCA GGCTGCCCTG 
AAGGTCCTCG CCCAGAAAGC CAGTGAGGCT CAGCCTCCTG TTGCCAGGAC CCAGCCTTCA 
AGTGGGGTTG ACAGTGCTGT GGGAACACTC CCTGCAACAA GTCCCCAGAG CACCTCCGTC 
CAGGCCAAAG GGACCAACAA GCTCAGAAAA CCTAAGCTTC CTGAGGTCCA GCAGGCCACC 
AAAGCCCCTG AGAGCTCAGA TGACAGTGAG GACAGCAGCG ACAGTTCTTC AGGGAGTGAG 
GAAGATGGTG AAGGGCCCCA GGGGGCCAAG TCAGCCCACA CGCTGGTAGG TCCCACCCCC 
TCCAGGACAG AGACCCTGGT GGAGGAGACC GCAGCAGAGT CCAGCGAGGA TGATGTGGTG 
GCGCCATCCC AGTCTCTCCT CTCAGGTTAT ATGACCCCTG GACTAACCCC AGCCAATTCC 
CAGGCCTCAA AAGCCACTCC CAAGCTAGAC TCCAGCCCCT CAGTTTCCTC TACTCTGGCC 
GCCAAAGATG ACCCAGATGG CAAGCAGGAG GCAAAGCCCC AACAGGCAGC AGGCATGTTG 
TCCCCTAAAA CAGGTGGAAA AGAGGCTGCT TCAGGCACCA CACCTCAGAA GTCCCGGAAG 
CCCAAGAAAG GGGCTGGGAA CCCCCAAGCC TCAACCCTGG CGCTGCAAAG CAACATCACC 
CAGTGCCTCC TGGGCCAACC CTGGCCCCTG AATGAGGCCC AGGTGCAGGC CTCAGTGGTG 
AAGGTCCTGA CTGAGCTGCT GGAACAGGAA AGAAAGAAGG TGGTGGACAC CACCAAGGAG 
AGCAGCAGGA AGGGCTGGGA GAGCCGCAAG CGGAAGCTAT CGGGAGACCA GCCAGCTGCC 
AGGACCCCCA GGAGCAAGAA GAAGAAGAAG CTGGGGGCCG GGGAAGGTGG GGAGGCCTCT 
GTTTCCCCAG AAAAGACCTC CACGACTTCC AAGGGGAAAG CAAAGAGAGA CAAAGCAAGT 
GGTGATGTCA AGGAGAAGAA AGGGAAGGGG TCTCTTGGCT CCCAAGGGGC CAAGGACGAG 
CCAGAAGAGG AGCTTCAGAA GGGGATGGGG ACGGTTGAAG GTGGAGATCA AAGCAACCCA 
AAGAGCAAGA AGGAGAAGAA GAAATCCGAC AAGAGAAAAA AAGACAAAGA AAAAAAAGAA 
AAGAAGAAGA AAGCAAAAAA GGCCTCAACC AAAGATTCTG AGTCACCGTC CCAGAAGAAA 
AAGAAGAAAA AGAAGAAGAC AGCAGAGCAG ACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( NM_001135245.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001128717.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 6, mRNA.

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CloneID	OHu28310
Clone ID Related Accession (Same CDS sequence)	NM_001135245.1 , NM_001135245.2
Accession Version	NM_001135245.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4239bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-26
Organism	Homo sapiens(human)
Product	treacle protein isoform f
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011372.7, BC027252.1, AK299167.1, U76366.1 and BM664489.1. On Jun 2, 2019 this sequence version replaced NM_001135245.1. Transcript Variant: This variant (6) lacks the alternate in-frame exon 7, also known as VIa, in the 5' coding region and uses an alternate in-frame splice site in the central coding region, compared to variant 4. The resulting isoform (f) lacks an internal segment, contains a one residue insertion, and has the same N- and C-termini, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGGT GATTAAACCC 
CCTCTGATTT TTGTCGACCC TAATCGTAGT CCAGCTGGCC CAGCTGCTAC ACCCGCACAA 
GCCCAGGCTG CAAGCACCCC GAGGAAGGCC CGAGCCTCGG AGAGCACAGC CAGGAGCTCC 
TCCTCCGAGA GCGAGGATGA GGACGTGATC CCCGCTACAC AGTGCTTGAC TCCTGGCATC 
AGAACCAATG TGGTGACCAT GCCCACTGCC CACCCAAGAA TAGCCCCCAA AGCCAGCATG 
GCTGGGGCCA GCAGCAGCAA GGAGTCCAGT CGGATATCAG ATGGCAAGAA ACAGGAGGGA 
CCAGCCACTC AGGTGTCAAA GAAGAACCCA GCTTCCCTCC CACTGACCCA GGCTGCCCTG 
AAGGTCCTCG CCCAGAAAGC CAGTGAGGCT CAGCCTCCTG TTGCCAGGAC CCAGCCTTCA 
AGTGGGGTTG ACAGTGCTGT GGGAACACTC CCTGCAACAA GTCCCCAGAG CACCTCCGTC 
CAGGCCAAAG GGACCAACAA GCTCAGAAAA CCTAAGCTTC CTGAGGTCCA GCAGGCCACC 
AAAGCCCCTG AGAGCTCAGA TGACAGTGAG GACAGCAGCG ACAGTTCTTC AGGGAGTGAG 
GAAGATGGTG AAGGGCCCCA GGGGGCCAAG TCAGCCCACA CGCTGGTAGG TCCCACCCCC 
TCCAGGACAG AGACCCTGGT GGAGGAGACC GCAGCAGAGT CCAGCGAGGA TGATGTGGTG 
GCGCCATCCC AGTCTCTCCT CTCAGGTTAT ATGACCCCTG GACTAACCCC AGCCAATTCC 
CAGGCCTCAA AAGCCACTCC CAAGCTAGAC TCCAGCCCCT CAGTTTCCTC TACTCTGGCC 
GCCAAAGATG ACCCAGATGG CAAGCAGGAG GCAAAGCCCC AACAGGCAGC AGGCATGTTG 
TCCCCTAAAA CAGGTGGAAA AGAGGCTGCT TCAGGCACCA CACCTCAGAA GTCCCGGAAG 
CCCAAGAAAG GGGCTGGGAA CCCCCAAGCC TCAACCCTGG CGCTGCAAAG CAACATCACC 
CAGTGCCTCC TGGGCCAACC CTGGCCCCTG AATGAGGCCC AGGTGCAGGC CTCAGTGGTG 
AAGGTCCTGA CTGAGCTGCT GGAACAGGAA AGAAAGAAGG TGGTGGACAC CACCAAGGAG 
AGCAGCAGGA AGGGCTGGGA GAGCCGCAAG CGGAAGCTAT CGGGAGACCA GCCAGCTGCC 
AGGACCCCCA GGAGCAAGAA GAAGAAGAAG CTGGGGGCCG GGGAAGGTGG GGAGGCCTCT 
GTTTCCCCAG AAAAGACCTC CACGACTTCC AAGGGGAAAG CAAAGAGAGA CAAAGCAAGT 
GGTGATGTCA AGGAGAAGAA AGGGAAGGGG TCTCTTGGCT CCCAAGGGGC CAAGGACGAG 
CCAGAAGAGG AGCTTCAGAA GGGGATGGGG ACGGTTGAAG GTGGAGATCA AAGCAACCCA 
AAGAGCAAGA AGGAGAAGAA GAAATCCGAC AAGAGAAAAA AAGACAAAGA AAAAAAAGAA 
AAGAAGAAGA AAGCAAAAAA GGCCTCAACC AAAGATTCTG AGTCACCGTC CCAGAAGAAA 
AAGAAGAAAA AGAAGAAGAC AGCAGAGCAG ACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001128717.1
CDS	51..4289
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	NM_001135245.2
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001135245.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGGT GATTAAACCC 
CCTCTGATTT TTGTCGACCC TAATCGTAGT CCAGCTGGCC CAGCTGCTAC ACCCGCACAA 
GCCCAGGCTG CAAGCACCCC GAGGAAGGCC CGAGCCTCGG AGAGCACAGC CAGGAGCTCC 
TCCTCCGAGA GCGAGGATGA GGACGTGATC CCCGCTACAC AGTGCTTGAC TCCTGGCATC 
AGAACCAATG TGGTGACCAT GCCCACTGCC CACCCAAGAA TAGCCCCCAA AGCCAGCATG 
GCTGGGGCCA GCAGCAGCAA GGAGTCCAGT CGGATATCAG ATGGCAAGAA ACAGGAGGGA 
CCAGCCACTC AGGTGTCAAA GAAGAACCCA GCTTCCCTCC CACTGACCCA GGCTGCCCTG 
AAGGTCCTCG CCCAGAAAGC CAGTGAGGCT CAGCCTCCTG TTGCCAGGAC CCAGCCTTCA 
AGTGGGGTTG ACAGTGCTGT GGGAACACTC CCTGCAACAA GTCCCCAGAG CACCTCCGTC 
CAGGCCAAAG GGACCAACAA GCTCAGAAAA CCTAAGCTTC CTGAGGTCCA GCAGGCCACC 
AAAGCCCCTG AGAGCTCAGA TGACAGTGAG GACAGCAGCG ACAGTTCTTC AGGGAGTGAG 
GAAGATGGTG AAGGGCCCCA GGGGGCCAAG TCAGCCCACA CGCTGGTAGG TCCCACCCCC 
TCCAGGACAG AGACCCTGGT GGAGGAGACC GCAGCAGAGT CCAGCGAGGA TGATGTGGTG 
GCGCCATCCC AGTCTCTCCT CTCAGGTTAT ATGACCCCTG GACTAACCCC AGCCAATTCC 
CAGGCCTCAA AAGCCACTCC CAAGCTAGAC TCCAGCCCCT CAGTTTCCTC TACTCTGGCC 
GCCAAAGATG ACCCAGATGG CAAGCAGGAG GCAAAGCCCC AACAGGCAGC AGGCATGTTG 
TCCCCTAAAA CAGGTGGAAA AGAGGCTGCT TCAGGCACCA CACCTCAGAA GTCCCGGAAG 
CCCAAGAAAG GGGCTGGGAA CCCCCAAGCC TCAACCCTGG CGCTGCAAAG CAACATCACC 
CAGTGCCTCC TGGGCCAACC CTGGCCCCTG AATGAGGCCC AGGTGCAGGC CTCAGTGGTG 
AAGGTCCTGA CTGAGCTGCT GGAACAGGAA AGAAAGAAGG TGGTGGACAC CACCAAGGAG 
AGCAGCAGGA AGGGCTGGGA GAGCCGCAAG CGGAAGCTAT CGGGAGACCA GCCAGCTGCC 
AGGACCCCCA GGAGCAAGAA GAAGAAGAAG CTGGGGGCCG GGGAAGGTGG GGAGGCCTCT 
GTTTCCCCAG AAAAGACCTC CACGACTTCC AAGGGGAAAG CAAAGAGAGA CAAAGCAAGT 
GGTGATGTCA AGGAGAAGAA AGGGAAGGGG TCTCTTGGCT CCCAAGGGGC CAAGGACGAG 
CCAGAAGAGG AGCTTCAGAA GGGGATGGGG ACGGTTGAAG GTGGAGATCA AAGCAACCCA 
AAGAGCAAGA AGGAGAAGAA GAAATCCGAC AAGAGAAAAA AAGACAAAGA AAAAAAAGAA 
AAGAAGAAGA AAGCAAAAAA GGCCTCAACC AAAGATTCTG AGTCACCGTC CCAGAAGAAA 
AAGAAGAAAA AGAAGAAGAC AGCAGAGCAG ACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( NM_001008657.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001008657.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28312
Clone ID Related Accession (Same CDS sequence)	NM_001008657.2 , NM_001008657.3
Accession Version	NM_001008657.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-19
Organism	Homo sapiens(human)
Product	treacle protein isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC377932.1, AC011372.7, BC011764.2, AI939467.1 and AW135418.1. On Sep 30, 2008 this sequence version replaced NM_001008657.1. Transcript Variant: This variant (3) uses an alternate terminal exon and lacks 10 3' exons, compared to variant 4. The resulting isoform (c) has a significantly shorter and distinct C-terminus, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.240507.1, BC011764.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG ACCAGGAGTC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008657.1
CDS	109..2985
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQDQESS

Target ORF information:

RefSeq Version	NM_001008657.2
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008657.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG ACCAGGAGTC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( NM_001008657.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001008657.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu28312
Clone ID Related Accession (Same CDS sequence)	NM_001008657.2 , NM_001008657.3
Accession Version	NM_001008657.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	treacle protein isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC011372.7, BC011764.2, AI939467.1 and AW135418.1. On Jun 2, 2019 this sequence version replaced NM_001008657.2. Transcript Variant: This variant (3) uses an alternate terminal exon and lacks 10 3' exons, compared to variant 4. The resulting isoform (c) has a significantly shorter and distinct C-terminus, compared to isoform d. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.240507.1, BC014559.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG ACCAGGAGTC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001008657.1
CDS	51..2927
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQDQESS

Target ORF information:

RefSeq Version	NM_001008657.3
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001008657.3

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG ACCAGGAGTC TTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268502.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268559.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu39895
Clone ID Related Accession (Same CDS sequence)	XM_005268502.3 , XM_005268502.4
Accession Version	XM_005268502.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268502.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTGCCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCACCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGG TGAAGGGCCC 
CAGGGGGCCA AGTCAGCCCA CACGCTGGTA GGTCCCACCC CCTCCAGGAC AGAGACCCTG 
GTGGAGGAGA CCGCAGCAGA GTCCAGCGAG GATGATGTGG TGGCGCCATC CCAGTCTCTC 
CTCTCAGGTT ATATGACCCC TGGACTAACC CCAGCCAATT CCCAGGCCTC AAAAGCCACT 
CCCAAGCTAG ACTCCAGCCC CTCAGTTTCC TCTACTCTGG CCGCCAAAGA TGACCCAGAT 
GGCAAGCAGG AGGCAAAGCC CCAACAGGCA GCAGGCATGT TGTCCCCTAA AACAGGTGGA 
AAAGAGGCTG CTTCAGGCAC CACACCTCAG AAGTCCCGGA AGCCCAAGAA AGGGGCTGGG 
AACCCCCAAG CCTCAACCCT GGCGCTGCAA AGCAACATCA CCCAGTGCCT CCTGGGCCAA 
CCCTGGCCCC TGAATGAGGC CCAGGTGCAG GCCTCAGTGG TGAAGGTCCT GACTGAGCTG 
CTGGAACAGG AAAGAAAGAA GGTGGTGGAC ACCACCAAGG AGAGCAGCAG GAAGGGCTGG 
GAGAGCCGCA AGCGGAAGCT ATCGGGAGAC CAGCCAGCTG CCAGGACCCC CAGGAGCAAG 
AAGAAGAAGA AGCTGGGGGC CGGGGAAGGT GGGGAGGCCT CTGTTTCCCC AGAAAAGACC 
TCCACGACTT CCAAGGGGAA AGCAAAGAGA GACAAAGCAA GTGGTGATGT CAAGGAGAAG 
AAAGGGAAGG GGTCTCTTGG CTCCCAAGGG GCCAAGGACG AGCCAGAAGA GGAGCTTCAG 
AAGGGGATGG GGACGGTTGA AGGTGGAGAT CAAAGCAACC CAAAGAGCAA GAAGGAGAAG 
AAGAAATCCG ACAAGAGAAA AAAAGACAAA GAAAAAAAAG AAAAGAAGAA GAAAGCAAAA 
AAGGCCTCAA CCAAAGATTC TGAGTCACCG TCCCAGAAGA AAAAGAAGAA AAAGAAGAAG 
ACAGCAGAGC AGACTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268559.1
CDS	46..4626
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005268502.3
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268502.3

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTGCCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCACCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGG TGAAGGGCCC 
CAGGGGGCCA AGTCAGCCCA CACGCTGGTA GGTCCCACCC CCTCCAGGAC AGAGACCCTG 
GTGGAGGAGA CCGCAGCAGA GTCCAGCGAG GATGATGTGG TGGCGCCATC CCAGTCTCTC 
CTCTCAGGTT ATATGACCCC TGGACTAACC CCAGCCAATT CCCAGGCCTC AAAAGCCACT 
CCCAAGCTAG ACTCCAGCCC CTCAGTTTCC TCTACTCTGG CCGCCAAAGA TGACCCAGAT 
GGCAAGCAGG AGGCAAAGCC CCAACAGGCA GCAGGCATGT TGTCCCCTAA AACAGGTGGA 
AAAGAGGCTG CTTCAGGCAC CACACCTCAG AAGTCCCGGA AGCCCAAGAA AGGGGCTGGG 
AACCCCCAAG CCTCAACCCT GGCGCTGCAA AGCAACATCA CCCAGTGCCT CCTGGGCCAA 
CCCTGGCCCC TGAATGAGGC CCAGGTGCAG GCCTCAGTGG TGAAGGTCCT GACTGAGCTG 
CTGGAACAGG AAAGAAAGAA GGTGGTGGAC ACCACCAAGG AGAGCAGCAG GAAGGGCTGG 
GAGAGCCGCA AGCGGAAGCT ATCGGGAGAC CAGCCAGCTG CCAGGACCCC CAGGAGCAAG 
AAGAAGAAGA AGCTGGGGGC CGGGGAAGGT GGGGAGGCCT CTGTTTCCCC AGAAAAGACC 
TCCACGACTT CCAAGGGGAA AGCAAAGAGA GACAAAGCAA GTGGTGATGT CAAGGAGAAG 
AAAGGGAAGG GGTCTCTTGG CTCCCAAGGG GCCAAGGACG AGCCAGAAGA GGAGCTTCAG 
AAGGGGATGG GGACGGTTGA AGGTGGAGAT CAAAGCAACC CAAAGAGCAA GAAGGAGAAG 
AAGAAATCCG ACAAGAGAAA AAAAGACAAA GAAAAAAAAG AAAAGAAGAA GAAAGCAAAA 
AAGGCCTCAA CCAAAGATTC TGAGTCACCG TCCCAGAAGA AAAAGAAGAA AAAGAAGAAG 
ACAGCAGAGC AGACTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268502.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268559.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu39895
Clone ID Related Accession (Same CDS sequence)	XM_005268502.3 , XM_005268502.4
Accession Version	XM_005268502.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4581bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005268502.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTGCCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCACCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGG TGAAGGGCCC 
CAGGGGGCCA AGTCAGCCCA CACGCTGGTA GGTCCCACCC CCTCCAGGAC AGAGACCCTG 
GTGGAGGAGA CCGCAGCAGA GTCCAGCGAG GATGATGTGG TGGCGCCATC CCAGTCTCTC 
CTCTCAGGTT ATATGACCCC TGGACTAACC CCAGCCAATT CCCAGGCCTC AAAAGCCACT 
CCCAAGCTAG ACTCCAGCCC CTCAGTTTCC TCTACTCTGG CCGCCAAAGA TGACCCAGAT 
GGCAAGCAGG AGGCAAAGCC CCAACAGGCA GCAGGCATGT TGTCCCCTAA AACAGGTGGA 
AAAGAGGCTG CTTCAGGCAC CACACCTCAG AAGTCCCGGA AGCCCAAGAA AGGGGCTGGG 
AACCCCCAAG CCTCAACCCT GGCGCTGCAA AGCAACATCA CCCAGTGCCT CCTGGGCCAA 
CCCTGGCCCC TGAATGAGGC CCAGGTGCAG GCCTCAGTGG TGAAGGTCCT GACTGAGCTG 
CTGGAACAGG AAAGAAAGAA GGTGGTGGAC ACCACCAAGG AGAGCAGCAG GAAGGGCTGG 
GAGAGCCGCA AGCGGAAGCT ATCGGGAGAC CAGCCAGCTG CCAGGACCCC CAGGAGCAAG 
AAGAAGAAGA AGCTGGGGGC CGGGGAAGGT GGGGAGGCCT CTGTTTCCCC AGAAAAGACC 
TCCACGACTT CCAAGGGGAA AGCAAAGAGA GACAAAGCAA GTGGTGATGT CAAGGAGAAG 
AAAGGGAAGG GGTCTCTTGG CTCCCAAGGG GCCAAGGACG AGCCAGAAGA GGAGCTTCAG 
AAGGGGATGG GGACGGTTGA AGGTGGAGAT CAAAGCAACC CAAAGAGCAA GAAGGAGAAG 
AAGAAATCCG ACAAGAGAAA AAAAGACAAA GAAAAAAAAG AAAAGAAGAA GAAAGCAAAA 
AAGGCCTCAA CCAAAGATTC TGAGTCACCG TCCCAGAAGA AAAAGAAGAA AAAGAAGAAG 
ACAGCAGAGC AGACTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268559.1
CDS	48..4628
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005268502.4
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268502.4

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTGCCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCACCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGG TGAAGGGCCC 
CAGGGGGCCA AGTCAGCCCA CACGCTGGTA GGTCCCACCC CCTCCAGGAC AGAGACCCTG 
GTGGAGGAGA CCGCAGCAGA GTCCAGCGAG GATGATGTGG TGGCGCCATC CCAGTCTCTC 
CTCTCAGGTT ATATGACCCC TGGACTAACC CCAGCCAATT CCCAGGCCTC AAAAGCCACT 
CCCAAGCTAG ACTCCAGCCC CTCAGTTTCC TCTACTCTGG CCGCCAAAGA TGACCCAGAT 
GGCAAGCAGG AGGCAAAGCC CCAACAGGCA GCAGGCATGT TGTCCCCTAA AACAGGTGGA 
AAAGAGGCTG CTTCAGGCAC CACACCTCAG AAGTCCCGGA AGCCCAAGAA AGGGGCTGGG 
AACCCCCAAG CCTCAACCCT GGCGCTGCAA AGCAACATCA CCCAGTGCCT CCTGGGCCAA 
CCCTGGCCCC TGAATGAGGC CCAGGTGCAG GCCTCAGTGG TGAAGGTCCT GACTGAGCTG 
CTGGAACAGG AAAGAAAGAA GGTGGTGGAC ACCACCAAGG AGAGCAGCAG GAAGGGCTGG 
GAGAGCCGCA AGCGGAAGCT ATCGGGAGAC CAGCCAGCTG CCAGGACCCC CAGGAGCAAG 
AAGAAGAAGA AGCTGGGGGC CGGGGAAGGT GGGGAGGCCT CTGTTTCCCC AGAAAAGACC 
TCCACGACTT CCAAGGGGAA AGCAAAGAGA GACAAAGCAA GTGGTGATGT CAAGGAGAAG 
AAAGGGAAGG GGTCTCTTGG CTCCCAAGGG GCCAAGGACG AGCCAGAAGA GGAGCTTCAG 
AAGGGGATGG GGACGGTTGA AGGTGGAGAT CAAAGCAACC CAAAGAGCAA GAAGGAGAAG 
AAGAAATCCG ACAAGAGAAA AAAAGACAAA GAAAAAAAAG AAAAGAAGAA GAAAGCAAAA 
AAGGCCTCAA CCAAAGATTC TGAGTCACCG TCCCAGAAGA AAAAGAAGAA AAAGAAGAAG 
ACAGCAGAGC AGACTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268503.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268560.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.

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CloneID	OHu39896
Clone ID Related Accession (Same CDS sequence)	XM_005268503.3 , XM_005268503.4
Accession Version	XM_005268503.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268503.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG ACAGTAACTC CAAACCTGCC 
AGAAGCAAGA CCCTGGCCCC AGCACCCCCA GAGAGGAACA CGGAGGGGTC CTCGGAGAGC 
AGTGAGGAAG AGCTGCCACT GACCCAGGTG ATTAAACCCC CTCTGATTTT TGTCGACCCT 
AATCGTAGTC CAGCTGGCCC AGCTGCTACA CCCGCACAAG CCCAGGCTGC AAGCACCCCG 
AGGAAGGCCC GAGCCTCGGA GAGCACAGCC AGGAGCTCCT CCTCCGAGAG CGAGGATGAG 
GACGTGATCC CCGCTACACA GTGCTTGACT CCTGGCATCA GAACCAATGT GGTGACCATG 
CCCACTGCCC ACCCAAGAAT AGCCCCCAAA GCCAGCATGG CTGGGGCCAG CAGCAGCAAG 
GAGTCCAGTC GGATATCAGA TGGCAAGAAA CAGGAGGGAC CAGCCACTCA GGTGTCAAAG 
AAGAACCCAG CTTCCCTCCC ACTGACCCAG GCTGCCCTGA AGGTCCTCGC CCAGAAAGCC 
AGTGAGGCTC AGCCTCCTGT TGCCAGGACC CAGCCTTCAA GTGGGGTTGA CAGTGCTGTG 
GGAACACTCC CTGCAACAAG TCCCCAGAGC ACCTCCGTCC AGGCCAAAGG GACCAACAAG 
CTCAGAAAAC CTAAGCTTCC TGAGGTCCAG CAGGCCACCA AAGCCCCTGA GAGCTCAGAT 
GACAGTGAGG ACAGCAGCGA CAGTTCTTCA GGGAGTGAGG AAGATGGTGA AGGGCCCCAG 
GGGGCCAAGT CAGCCCACAC GCTGGTAGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCACCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GGAAGCTATC GGGAGACCAG CCAGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCGG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCAAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA CGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268560.1
CDS	46..4623
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005268503.3
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268503.3

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG ACAGTAACTC CAAACCTGCC 
AGAAGCAAGA CCCTGGCCCC AGCACCCCCA GAGAGGAACA CGGAGGGGTC CTCGGAGAGC 
AGTGAGGAAG AGCTGCCACT GACCCAGGTG ATTAAACCCC CTCTGATTTT TGTCGACCCT 
AATCGTAGTC CAGCTGGCCC AGCTGCTACA CCCGCACAAG CCCAGGCTGC AAGCACCCCG 
AGGAAGGCCC GAGCCTCGGA GAGCACAGCC AGGAGCTCCT CCTCCGAGAG CGAGGATGAG 
GACGTGATCC CCGCTACACA GTGCTTGACT CCTGGCATCA GAACCAATGT GGTGACCATG 
CCCACTGCCC ACCCAAGAAT AGCCCCCAAA GCCAGCATGG CTGGGGCCAG CAGCAGCAAG 
GAGTCCAGTC GGATATCAGA TGGCAAGAAA CAGGAGGGAC CAGCCACTCA GGTGTCAAAG 
AAGAACCCAG CTTCCCTCCC ACTGACCCAG GCTGCCCTGA AGGTCCTCGC CCAGAAAGCC 
AGTGAGGCTC AGCCTCCTGT TGCCAGGACC CAGCCTTCAA GTGGGGTTGA CAGTGCTGTG 
GGAACACTCC CTGCAACAAG TCCCCAGAGC ACCTCCGTCC AGGCCAAAGG GACCAACAAG 
CTCAGAAAAC CTAAGCTTCC TGAGGTCCAG CAGGCCACCA AAGCCCCTGA GAGCTCAGAT 
GACAGTGAGG ACAGCAGCGA CAGTTCTTCA GGGAGTGAGG AAGATGGTGA AGGGCCCCAG 
GGGGCCAAGT CAGCCCACAC GCTGGTAGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCACCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GGAAGCTATC GGGAGACCAG CCAGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCGG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCAAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA CGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268503.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268560.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.

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CloneID	OHu39896
Clone ID Related Accession (Same CDS sequence)	XM_005268503.3 , XM_005268503.4
Accession Version	XM_005268503.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005268503.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG ACAGTAACTC CAAACCTGCC 
AGAAGCAAGA CCCTGGCCCC AGCACCCCCA GAGAGGAACA CGGAGGGGTC CTCGGAGAGC 
AGTGAGGAAG AGCTGCCACT GACCCAGGTG ATTAAACCCC CTCTGATTTT TGTCGACCCT 
AATCGTAGTC CAGCTGGCCC AGCTGCTACA CCCGCACAAG CCCAGGCTGC AAGCACCCCG 
AGGAAGGCCC GAGCCTCGGA GAGCACAGCC AGGAGCTCCT CCTCCGAGAG CGAGGATGAG 
GACGTGATCC CCGCTACACA GTGCTTGACT CCTGGCATCA GAACCAATGT GGTGACCATG 
CCCACTGCCC ACCCAAGAAT AGCCCCCAAA GCCAGCATGG CTGGGGCCAG CAGCAGCAAG 
GAGTCCAGTC GGATATCAGA TGGCAAGAAA CAGGAGGGAC CAGCCACTCA GGTGTCAAAG 
AAGAACCCAG CTTCCCTCCC ACTGACCCAG GCTGCCCTGA AGGTCCTCGC CCAGAAAGCC 
AGTGAGGCTC AGCCTCCTGT TGCCAGGACC CAGCCTTCAA GTGGGGTTGA CAGTGCTGTG 
GGAACACTCC CTGCAACAAG TCCCCAGAGC ACCTCCGTCC AGGCCAAAGG GACCAACAAG 
CTCAGAAAAC CTAAGCTTCC TGAGGTCCAG CAGGCCACCA AAGCCCCTGA GAGCTCAGAT 
GACAGTGAGG ACAGCAGCGA CAGTTCTTCA GGGAGTGAGG AAGATGGTGA AGGGCCCCAG 
GGGGCCAAGT CAGCCCACAC GCTGGTAGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCACCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GGAAGCTATC GGGAGACCAG CCAGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCGG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCAAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA CGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268560.1
CDS	48..4625
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005268503.4
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268503.4

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG ACAGTAACTC CAAACCTGCC 
AGAAGCAAGA CCCTGGCCCC AGCACCCCCA GAGAGGAACA CGGAGGGGTC CTCGGAGAGC 
AGTGAGGAAG AGCTGCCACT GACCCAGGTG ATTAAACCCC CTCTGATTTT TGTCGACCCT 
AATCGTAGTC CAGCTGGCCC AGCTGCTACA CCCGCACAAG CCCAGGCTGC AAGCACCCCG 
AGGAAGGCCC GAGCCTCGGA GAGCACAGCC AGGAGCTCCT CCTCCGAGAG CGAGGATGAG 
GACGTGATCC CCGCTACACA GTGCTTGACT CCTGGCATCA GAACCAATGT GGTGACCATG 
CCCACTGCCC ACCCAAGAAT AGCCCCCAAA GCCAGCATGG CTGGGGCCAG CAGCAGCAAG 
GAGTCCAGTC GGATATCAGA TGGCAAGAAA CAGGAGGGAC CAGCCACTCA GGTGTCAAAG 
AAGAACCCAG CTTCCCTCCC ACTGACCCAG GCTGCCCTGA AGGTCCTCGC CCAGAAAGCC 
AGTGAGGCTC AGCCTCCTGT TGCCAGGACC CAGCCTTCAA GTGGGGTTGA CAGTGCTGTG 
GGAACACTCC CTGCAACAAG TCCCCAGAGC ACCTCCGTCC AGGCCAAAGG GACCAACAAG 
CTCAGAAAAC CTAAGCTTCC TGAGGTCCAG CAGGCCACCA AAGCCCCTGA GAGCTCAGAT 
GACAGTGAGG ACAGCAGCGA CAGTTCTTCA GGGAGTGAGG AAGATGGTGA AGGGCCCCAG 
GGGGCCAAGT CAGCCCACAC GCTGGTAGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCACCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GGAAGCTATC GGGAGACCAG CCAGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCGG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCAAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA CGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268504.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268561.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu39897
Clone ID Related Accession (Same CDS sequence)	XM_005268504.3 , XM_005268504.4
Accession Version	XM_005268504.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268504.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTGCCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCACCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGG TGAAGGGCCC 
CAGGGGGCCA AGTCAGCCCA CACGCTGGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCACCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GGAAGCTATC GGGAGACCAG CCAGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCGG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCAAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA CGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268561.1
CDS	46..4623
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005268504.3
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268504.3

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTGCCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCACCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGG TGAAGGGCCC 
CAGGGGGCCA AGTCAGCCCA CACGCTGGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCACCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GGAAGCTATC GGGAGACCAG CCAGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCGG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCAAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA CGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268504.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268561.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu39897
Clone ID Related Accession (Same CDS sequence)	XM_005268504.3 , XM_005268504.4
Accession Version	XM_005268504.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4578bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005268504.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTGCCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCACCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGG TGAAGGGCCC 
CAGGGGGCCA AGTCAGCCCA CACGCTGGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCACCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GGAAGCTATC GGGAGACCAG CCAGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCGG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCAAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA CGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268561.1
CDS	48..4625
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005268504.4
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268504.4

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTGCCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCACCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGG TGAAGGGCCC 
CAGGGGGCCA AGTCAGCCCA CACGCTGGGT CCCACCCCCT CCAGGACAGA GACCCTGGTG 
GAGGAGACCG CAGCAGAGTC CAGCGAGGAT GATGTGGTGG CGCCATCCCA GTCTCTCCTC 
TCAGGTTATA TGACCCCTGG ACTAACCCCA GCCAATTCCC AGGCCTCAAA AGCCACTCCC 
AAGCTAGACT CCAGCCCCTC AGTTTCCTCT ACTCTGGCCG CCAAAGATGA CCCAGATGGC 
AAGCAGGAGG CAAAGCCCCA ACAGGCAGCA GGCATGTTGT CCCCTAAAAC AGGTGGAAAA 
GAGGCTGCTT CAGGCACCAC ACCTCAGAAG TCCCGGAAGC CCAAGAAAGG GGCTGGGAAC 
CCCCAAGCCT CAACCCTGGC GCTGCAAAGC AACATCACCC AGTGCCTCCT GGGCCAACCC 
TGGCCCCTGA ATGAGGCCCA GGTGCAGGCC TCAGTGGTGA AGGTCCTGAC TGAGCTGCTG 
GAACAGGAAA GAAAGAAGGT GGTGGACACC ACCAAGGAGA GCAGCAGGAA GGGCTGGGAG 
AGCCGCAAGC GGAAGCTATC GGGAGACCAG CCAGCTGCCA GGACCCCCAG GAGCAAGAAG 
AAGAAGAAGC TGGGGGCCGG GGAAGGTGGG GAGGCCTCTG TTTCCCCAGA AAAGACCTCC 
ACGACTTCCA AGGGGAAAGC AAAGAGAGAC AAAGCAAGTG GTGATGTCAA GGAGAAGAAA 
GGGAAGGGGT CTCTTGGCTC CCAAGGGGCC AAGGACGAGC CAGAAGAGGA GCTTCAGAAG 
GGGATGGGGA CGGTTGAAGG TGGAGATCAA AGCAACCCAA AGAGCAAGAA GGAGAAGAAG 
AAATCCGACA AGAGAAAAAA AGACAAAGAA AAAAAAGAAA AGAAGAAGAA AGCAAAAAAG 
GCCTCAACCA AAGATTCTGA GTCACCGTCC CAGAAGAAAA AGAAGAAAAA GAAGAAGACA 
GCAGAGCAGA CTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268505.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268562.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.

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CloneID	OHu39898
Clone ID Related Accession (Same CDS sequence)	NM_001371623.1 , XM_005268505.4 , XM_005268505.3
Accession Version	XM_005268505.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	treacle protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005268505.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268562.1
CDS	48..4517
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005268505.4
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268505.4

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268505.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268562.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.

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CloneID	OHu39898
Clone ID Related Accession (Same CDS sequence)	NM_001371623.1 , XM_005268505.4 , XM_005268505.3
Accession Version	XM_005268505.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268505.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268562.1
CDS	46..4515
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005268505.3
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268505.3

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268506.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268563.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu39899
Clone ID Related Accession (Same CDS sequence)	XM_005268506.4 , XM_005268506.3
Accession Version	XM_005268506.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005268506.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTTGAC 
AGTGCTGTGG GAACACTCCC TGCAACAAGT CCCCAGAGCA CCTCCGTCCA GGCCAAAGGG 
ACCAACAAGC TCAGAAAACC TAAGCTTCCT GAGGTCCAGC AGGCCACCAA AGCCCCTGAG 
AGCTCAGATG ACAGTGAGGA CAGCAGCGAC AGTTCTTCAG GGAGTGAGGA AGATGGTGAA 
GGGCCCCAGG GGGCCAAGTC AGCCCACACG CTGGTAGGTC CCACCCCCTC CAGGACAGAG 
ACCCTGGTGG AGGAGACCGC AGCAGAGTCC AGCGAGGATG ATGTGGTGGC GCCATCCCAG 
TCTCTCCTCT CAGGTTATAT GACCCCTGGA CTAACCCCAG CCAATTCCCA GGCCTCAAAA 
GCCACTCCCA AGCTAGACTC CAGCCCCTCA GTTTCCTCTA CTCTGGCCGC CAAAGATGAC 
CCAGATGGCA AGCAGGAGGC AAAGCCCCAA CAGGCAGCAG GCATGTTGTC CCCTAAAACA 
GGTGGAAAAG AGGCTGCTTC AGGCACCACA CCTCAGAAGT CCCGGAAGCC CAAGAAAGGG 
GCTGGGAACC CCCAAGCCTC AACCCTGGCG CTGCAAAGCA ACATCACCCA GTGCCTCCTG 
GGCCAACCCT GGCCCCTGAA TGAGGCCCAG GTGCAGGCCT CAGTGGTGAA GGTCCTGACT 
GAGCTGCTGG AACAGGAAAG AAAGAAGGTG GTGGACACCA CCAAGGAGAG CAGCAGGAAG 
GGCTGGGAGA GCCGCAAGCG GAAGCTATCG GGAGACCAGC CAGCTGCCAG GACCCCCAGG 
AGCAAGAAGA AGAAGAAGCT GGGGGCCGGG GAAGGTGGGG AGGCCTCTGT TTCCCCAGAA 
AAGACCTCCA CGACTTCCAA GGGGAAAGCA AAGAGAGACA AAGCAAGTGG TGATGTCAAG 
GAGAAGAAAG GGAAGGGGTC TCTTGGCTCC CAAGGGGCCA AGGACGAGCC AGAAGAGGAG 
CTTCAGAAGG GGATGGGGAC GGTTGAAGGT GGAGATCAAA GCAACCCAAA GAGCAAGAAG 
GAGAAGAAGA AATCCGACAA GAGAAAAAAA GACAAAGAAA AAAAAGAAAA GAAGAAGAAA 
GCAAAAAAGG CCTCAACCAA AGATTCTGAG TCACCGTCCC AGAAGAAAAA GAAGAAAAAG 
AAGAAGACAG CAGAGCAGAC TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268563.1
CDS	48..4514
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005268506.4
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268506.4

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTTGAC 
AGTGCTGTGG GAACACTCCC TGCAACAAGT CCCCAGAGCA CCTCCGTCCA GGCCAAAGGG 
ACCAACAAGC TCAGAAAACC TAAGCTTCCT GAGGTCCAGC AGGCCACCAA AGCCCCTGAG 
AGCTCAGATG ACAGTGAGGA CAGCAGCGAC AGTTCTTCAG GGAGTGAGGA AGATGGTGAA 
GGGCCCCAGG GGGCCAAGTC AGCCCACACG CTGGTAGGTC CCACCCCCTC CAGGACAGAG 
ACCCTGGTGG AGGAGACCGC AGCAGAGTCC AGCGAGGATG ATGTGGTGGC GCCATCCCAG 
TCTCTCCTCT CAGGTTATAT GACCCCTGGA CTAACCCCAG CCAATTCCCA GGCCTCAAAA 
GCCACTCCCA AGCTAGACTC CAGCCCCTCA GTTTCCTCTA CTCTGGCCGC CAAAGATGAC 
CCAGATGGCA AGCAGGAGGC AAAGCCCCAA CAGGCAGCAG GCATGTTGTC CCCTAAAACA 
GGTGGAAAAG AGGCTGCTTC AGGCACCACA CCTCAGAAGT CCCGGAAGCC CAAGAAAGGG 
GCTGGGAACC CCCAAGCCTC AACCCTGGCG CTGCAAAGCA ACATCACCCA GTGCCTCCTG 
GGCCAACCCT GGCCCCTGAA TGAGGCCCAG GTGCAGGCCT CAGTGGTGAA GGTCCTGACT 
GAGCTGCTGG AACAGGAAAG AAAGAAGGTG GTGGACACCA CCAAGGAGAG CAGCAGGAAG 
GGCTGGGAGA GCCGCAAGCG GAAGCTATCG GGAGACCAGC CAGCTGCCAG GACCCCCAGG 
AGCAAGAAGA AGAAGAAGCT GGGGGCCGGG GAAGGTGGGG AGGCCTCTGT TTCCCCAGAA 
AAGACCTCCA CGACTTCCAA GGGGAAAGCA AAGAGAGACA AAGCAAGTGG TGATGTCAAG 
GAGAAGAAAG GGAAGGGGTC TCTTGGCTCC CAAGGGGCCA AGGACGAGCC AGAAGAGGAG 
CTTCAGAAGG GGATGGGGAC GGTTGAAGGT GGAGATCAAA GCAACCCAAA GAGCAAGAAG 
GAGAAGAAGA AATCCGACAA GAGAAAAAAA GACAAAGAAA AAAAAGAAAA GAAGAAGAAA 
GCAAAAAAGG CCTCAACCAA AGATTCTGAG TCACCGTCCC AGAAGAAAAA GAAGAAAAAG 
AAGAAGACAG CAGAGCAGAC TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268506.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268563.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu39899
Clone ID Related Accession (Same CDS sequence)	XM_005268506.4 , XM_005268506.3
Accession Version	XM_005268506.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4467bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268506.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTTGAC 
AGTGCTGTGG GAACACTCCC TGCAACAAGT CCCCAGAGCA CCTCCGTCCA GGCCAAAGGG 
ACCAACAAGC TCAGAAAACC TAAGCTTCCT GAGGTCCAGC AGGCCACCAA AGCCCCTGAG 
AGCTCAGATG ACAGTGAGGA CAGCAGCGAC AGTTCTTCAG GGAGTGAGGA AGATGGTGAA 
GGGCCCCAGG GGGCCAAGTC AGCCCACACG CTGGTAGGTC CCACCCCCTC CAGGACAGAG 
ACCCTGGTGG AGGAGACCGC AGCAGAGTCC AGCGAGGATG ATGTGGTGGC GCCATCCCAG 
TCTCTCCTCT CAGGTTATAT GACCCCTGGA CTAACCCCAG CCAATTCCCA GGCCTCAAAA 
GCCACTCCCA AGCTAGACTC CAGCCCCTCA GTTTCCTCTA CTCTGGCCGC CAAAGATGAC 
CCAGATGGCA AGCAGGAGGC AAAGCCCCAA CAGGCAGCAG GCATGTTGTC CCCTAAAACA 
GGTGGAAAAG AGGCTGCTTC AGGCACCACA CCTCAGAAGT CCCGGAAGCC CAAGAAAGGG 
GCTGGGAACC CCCAAGCCTC AACCCTGGCG CTGCAAAGCA ACATCACCCA GTGCCTCCTG 
GGCCAACCCT GGCCCCTGAA TGAGGCCCAG GTGCAGGCCT CAGTGGTGAA GGTCCTGACT 
GAGCTGCTGG AACAGGAAAG AAAGAAGGTG GTGGACACCA CCAAGGAGAG CAGCAGGAAG 
GGCTGGGAGA GCCGCAAGCG GAAGCTATCG GGAGACCAGC CAGCTGCCAG GACCCCCAGG 
AGCAAGAAGA AGAAGAAGCT GGGGGCCGGG GAAGGTGGGG AGGCCTCTGT TTCCCCAGAA 
AAGACCTCCA CGACTTCCAA GGGGAAAGCA AAGAGAGACA AAGCAAGTGG TGATGTCAAG 
GAGAAGAAAG GGAAGGGGTC TCTTGGCTCC CAAGGGGCCA AGGACGAGCC AGAAGAGGAG 
CTTCAGAAGG GGATGGGGAC GGTTGAAGGT GGAGATCAAA GCAACCCAAA GAGCAAGAAG 
GAGAAGAAGA AATCCGACAA GAGAAAAAAA GACAAAGAAA AAAAAGAAAA GAAGAAGAAA 
GCAAAAAAGG CCTCAACCAA AGATTCTGAG TCACCGTCCC AGAAGAAAAA GAAGAAAAAG 
AAGAAGACAG CAGAGCAGAC TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268563.1
CDS	46..4512
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005268506.3
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268506.3

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTTGAC 
AGTGCTGTGG GAACACTCCC TGCAACAAGT CCCCAGAGCA CCTCCGTCCA GGCCAAAGGG 
ACCAACAAGC TCAGAAAACC TAAGCTTCCT GAGGTCCAGC AGGCCACCAA AGCCCCTGAG 
AGCTCAGATG ACAGTGAGGA CAGCAGCGAC AGTTCTTCAG GGAGTGAGGA AGATGGTGAA 
GGGCCCCAGG GGGCCAAGTC AGCCCACACG CTGGTAGGTC CCACCCCCTC CAGGACAGAG 
ACCCTGGTGG AGGAGACCGC AGCAGAGTCC AGCGAGGATG ATGTGGTGGC GCCATCCCAG 
TCTCTCCTCT CAGGTTATAT GACCCCTGGA CTAACCCCAG CCAATTCCCA GGCCTCAAAA 
GCCACTCCCA AGCTAGACTC CAGCCCCTCA GTTTCCTCTA CTCTGGCCGC CAAAGATGAC 
CCAGATGGCA AGCAGGAGGC AAAGCCCCAA CAGGCAGCAG GCATGTTGTC CCCTAAAACA 
GGTGGAAAAG AGGCTGCTTC AGGCACCACA CCTCAGAAGT CCCGGAAGCC CAAGAAAGGG 
GCTGGGAACC CCCAAGCCTC AACCCTGGCG CTGCAAAGCA ACATCACCCA GTGCCTCCTG 
GGCCAACCCT GGCCCCTGAA TGAGGCCCAG GTGCAGGCCT CAGTGGTGAA GGTCCTGACT 
GAGCTGCTGG AACAGGAAAG AAAGAAGGTG GTGGACACCA CCAAGGAGAG CAGCAGGAAG 
GGCTGGGAGA GCCGCAAGCG GAAGCTATCG GGAGACCAGC CAGCTGCCAG GACCCCCAGG 
AGCAAGAAGA AGAAGAAGCT GGGGGCCGGG GAAGGTGGGG AGGCCTCTGT TTCCCCAGAA 
AAGACCTCCA CGACTTCCAA GGGGAAAGCA AAGAGAGACA AAGCAAGTGG TGATGTCAAG 
GAGAAGAAAG GGAAGGGGTC TCTTGGCTCC CAAGGGGCCA AGGACGAGCC AGAAGAGGAG 
CTTCAGAAGG GGATGGGGAC GGTTGAAGGT GGAGATCAAA GCAACCCAAA GAGCAAGAAG 
GAGAAGAAGA AATCCGACAA GAGAAAAAAA GACAAAGAAA AAAAAGAAAA GAAGAAGAAA 
GCAAAAAAGG CCTCAACCAA AGATTCTGAG TCACCGTCCC AGAAGAAAAA GAAGAAAAAG 
AAGAAGACAG CAGAGCAGAC TGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268507.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268564.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.

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CloneID	OHu39900
Clone ID Related Accession (Same CDS sequence)	XM_005268507.4 , XM_005268507.3
Accession Version	XM_005268507.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005268507.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGAA GGACAGTAAC 
TCCAAACCTG CCAGAAGCAA GACCCTGGCC CCAGCACCCC CAGAGAGGAA CACGGAGGGG 
TCCTCGGAGA GCAGTGAGGA AGAGCTGCCA CTGACCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268564.1
CDS	48..4397
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005268507.4
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268507.4

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGAA GGACAGTAAC 
TCCAAACCTG CCAGAAGCAA GACCCTGGCC CCAGCACCCC CAGAGAGGAA CACGGAGGGG 
TCCTCGGAGA GCAGTGAGGA AGAGCTGCCA CTGACCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268507.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268564.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.

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CloneID	OHu39900
Clone ID Related Accession (Same CDS sequence)	XM_005268507.4 , XM_005268507.3
Accession Version	XM_005268507.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4350bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268507.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGAA GGACAGTAAC 
TCCAAACCTG CCAGAAGCAA GACCCTGGCC CCAGCACCCC CAGAGAGGAA CACGGAGGGG 
TCCTCGGAGA GCAGTGAGGA AGAGCTGCCA CTGACCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268564.1
CDS	46..4395
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_005268507.3
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268507.3

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGAA GGACAGTAAC 
TCCAAACCTG CCAGAAGCAA GACCCTGGCC CCAGCACCCC CAGAGAGGAA CACGGAGGGG 
TCCTCGGAGA GCAGTGAGGA AGAGCTGCCA CTGACCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268508.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268565.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu39901
Clone ID Related Accession (Same CDS sequence)	XM_005268508.4 , XM_005268508.3
Accession Version	XM_005268508.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005268508.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GACCAGGAGT CTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268565.1
CDS	48..3035
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQDQESS

Target ORF information:

RefSeq Version	XM_005268508.4
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268508.4

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GACCAGGAGT CTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268508.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268565.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu39901
Clone ID Related Accession (Same CDS sequence)	XM_005268508.4 , XM_005268508.3
Accession Version	XM_005268508.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2988bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268508.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GACCAGGAGT CTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268565.1
CDS	46..3033
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQDQESS

Target ORF information:

RefSeq Version	XM_005268508.3
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268508.3

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GACCAGGAGT CTTCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268509.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268566.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu39902
Clone ID Related Accession (Same CDS sequence)	XM_005268509.4 , XM_005268509.3
Accession Version	XM_005268509.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005268509.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG ACAGTAACTC CAAACCTGCC 
AGAAGCAAGA CCCTGGCCCC AGCACCCCCA GAGAGGAACA CGGAGGGGTC CTCGGAGAGC 
AGTGAGGAAG AGCTGCCACT GACCCAGGAC CAGGAGTCTT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268566.1
CDS	48..3032
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQDQESS

Target ORF information:

RefSeq Version	XM_005268509.4
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268509.4

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG ACAGTAACTC CAAACCTGCC 
AGAAGCAAGA CCCTGGCCCC AGCACCCCCA GAGAGGAACA CGGAGGGGTC CTCGGAGAGC 
AGTGAGGAAG AGCTGCCACT GACCCAGGAC CAGGAGTCTT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_005268509.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005268566.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu39902
Clone ID Related Accession (Same CDS sequence)	XM_005268509.4 , XM_005268509.3
Accession Version	XM_005268509.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2985bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005268509.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG ACAGTAACTC CAAACCTGCC 
AGAAGCAAGA CCCTGGCCCC AGCACCCCCA GAGAGGAACA CGGAGGGGTC CTCGGAGAGC 
AGTGAGGAAG AGCTGCCACT GACCCAGGAC CAGGAGTCTT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005268566.1
CDS	46..3030
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQDQESS

Target ORF information:

RefSeq Version	XM_005268509.3
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005268509.3

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG ACAGTAACTC CAAACCTGCC 
AGAAGCAAGA CCCTGGCCCC AGCACCCCCA GAGAGGAACA CGGAGGGGTC CTCGGAGAGC 
AGTGAGGAAG AGCTGCCACT GACCCAGGAC CAGGAGTCTT CTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_011537678.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535980.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.

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CloneID	OHu60933
Clone ID Related Accession (Same CDS sequence)	XM_011537678.2 , XM_011537678.3
Accession Version	XM_011537678.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011537678.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTGCCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCACCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGG TGAAGGGCCC 
CAGGGGGCCA AGTCAGCCCA CACGCTGGTA GGTCCCACCC CCTCCAGGAC AGAGACCCTG 
GTGGAGGAGA CCGCAGCAGA GTCCAGCGAG GATGATGTGG TGGCGCCATC CCAGTCTCTC 
CTCTCAGGTT ATATGACCCC TGGACTAACC CCAGCCAATT CCCAGGCCTC AAAAGCCACT 
CCCAAGCTAG ACTCCAGCCC CTCAGTTTCC TCTACTCTGG CCGCCAAAGA TGACCCAGAT 
GGCAAGCAGG AGGCAAAGCC CCAACAGGCA GCAGGCATGT TGTCCCCTAA AACAGGTGGA 
AAAGAGGCTG CTTCAGGCAC CACACCTCAG AAGTCCCGGA AGCCCAAGAA AGGGGCTGGG 
AACCCCCAAG CCTCAACCCT GGCGCTGCAA AGCAACATCA CCCAGTGCCT CCTGGGCCAA 
CCCTGGCCCC TGAATGAGGC CCAGGTGCAG GCCTCAGTGG TGAAGGTCCT GACTGAGCTG 
CTGGAACAGG AAAGAAAGAA GGTGGTGGAC ACCACCAAGG AGAGCAGCAG GAAGGGCTGG 
GAGAGCCGCA AGCGGAAGCT ATCGGGAGAC CAGCCAGCTG CCAGGACCCC CAGGAGCAAG 
AAGAAGAAGA AGCTGGGGGC CGGGGAAGGT GGGGAGGCCT CTGTTTCCCC AGAAAAGACC 
TCCACGACTT CCAAGGGGAA AGCAAAGAGA GACAAAGCAA GTGGTGATGT CAAGGAGAAG 
AAAGGGAAGG GGTCTCTTGG CTCCCAAGGG GCCAAGGACG AGCCAGAAGA GGAGCTTCAG 
AAGGGGATGG GGACGGTTGA AGGTGGAGAT CAAAGCAACC CAAAGAGCAA GAAGGAGAAG 
AAGAAATCCG ACAAGAGAAA AAAAGACAAA GAAAAAAAAG AAAAGAAGAA GAAAGCAAAA 
AAGGCCTCAA CCAAAGATTC TGAGTCACCG TCCCAGAAGA AAAAGAAGAA AAAGAAGAAG 
ACAGCAGAGC AGACTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535980.1
CDS	46..4446
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_011537678.2
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537678.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTGCCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCACCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGG TGAAGGGCCC 
CAGGGGGCCA AGTCAGCCCA CACGCTGGTA GGTCCCACCC CCTCCAGGAC AGAGACCCTG 
GTGGAGGAGA CCGCAGCAGA GTCCAGCGAG GATGATGTGG TGGCGCCATC CCAGTCTCTC 
CTCTCAGGTT ATATGACCCC TGGACTAACC CCAGCCAATT CCCAGGCCTC AAAAGCCACT 
CCCAAGCTAG ACTCCAGCCC CTCAGTTTCC TCTACTCTGG CCGCCAAAGA TGACCCAGAT 
GGCAAGCAGG AGGCAAAGCC CCAACAGGCA GCAGGCATGT TGTCCCCTAA AACAGGTGGA 
AAAGAGGCTG CTTCAGGCAC CACACCTCAG AAGTCCCGGA AGCCCAAGAA AGGGGCTGGG 
AACCCCCAAG CCTCAACCCT GGCGCTGCAA AGCAACATCA CCCAGTGCCT CCTGGGCCAA 
CCCTGGCCCC TGAATGAGGC CCAGGTGCAG GCCTCAGTGG TGAAGGTCCT GACTGAGCTG 
CTGGAACAGG AAAGAAAGAA GGTGGTGGAC ACCACCAAGG AGAGCAGCAG GAAGGGCTGG 
GAGAGCCGCA AGCGGAAGCT ATCGGGAGAC CAGCCAGCTG CCAGGACCCC CAGGAGCAAG 
AAGAAGAAGA AGCTGGGGGC CGGGGAAGGT GGGGAGGCCT CTGTTTCCCC AGAAAAGACC 
TCCACGACTT CCAAGGGGAA AGCAAAGAGA GACAAAGCAA GTGGTGATGT CAAGGAGAAG 
AAAGGGAAGG GGTCTCTTGG CTCCCAAGGG GCCAAGGACG AGCCAGAAGA GGAGCTTCAG 
AAGGGGATGG GGACGGTTGA AGGTGGAGAT CAAAGCAACC CAAAGAGCAA GAAGGAGAAG 
AAGAAATCCG ACAAGAGAAA AAAAGACAAA GAAAAAAAAG AAAAGAAGAA GAAAGCAAAA 
AAGGCCTCAA CCAAAGATTC TGAGTCACCG TCCCAGAAGA AAAAGAAGAA AAAGAAGAAG 
ACAGCAGAGC AGACTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_011537678.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011535980.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu60933
Clone ID Related Accession (Same CDS sequence)	XM_011537678.2 , XM_011537678.3
Accession Version	XM_011537678.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4401bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011537678.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTGCCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCACCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGG TGAAGGGCCC 
CAGGGGGCCA AGTCAGCCCA CACGCTGGTA GGTCCCACCC CCTCCAGGAC AGAGACCCTG 
GTGGAGGAGA CCGCAGCAGA GTCCAGCGAG GATGATGTGG TGGCGCCATC CCAGTCTCTC 
CTCTCAGGTT ATATGACCCC TGGACTAACC CCAGCCAATT CCCAGGCCTC AAAAGCCACT 
CCCAAGCTAG ACTCCAGCCC CTCAGTTTCC TCTACTCTGG CCGCCAAAGA TGACCCAGAT 
GGCAAGCAGG AGGCAAAGCC CCAACAGGCA GCAGGCATGT TGTCCCCTAA AACAGGTGGA 
AAAGAGGCTG CTTCAGGCAC CACACCTCAG AAGTCCCGGA AGCCCAAGAA AGGGGCTGGG 
AACCCCCAAG CCTCAACCCT GGCGCTGCAA AGCAACATCA CCCAGTGCCT CCTGGGCCAA 
CCCTGGCCCC TGAATGAGGC CCAGGTGCAG GCCTCAGTGG TGAAGGTCCT GACTGAGCTG 
CTGGAACAGG AAAGAAAGAA GGTGGTGGAC ACCACCAAGG AGAGCAGCAG GAAGGGCTGG 
GAGAGCCGCA AGCGGAAGCT ATCGGGAGAC CAGCCAGCTG CCAGGACCCC CAGGAGCAAG 
AAGAAGAAGA AGCTGGGGGC CGGGGAAGGT GGGGAGGCCT CTGTTTCCCC AGAAAAGACC 
TCCACGACTT CCAAGGGGAA AGCAAAGAGA GACAAAGCAA GTGGTGATGT CAAGGAGAAG 
AAAGGGAAGG GGTCTCTTGG CTCCCAAGGG GCCAAGGACG AGCCAGAAGA GGAGCTTCAG 
AAGGGGATGG GGACGGTTGA AGGTGGAGAT CAAAGCAACC CAAAGAGCAA GAAGGAGAAG 
AAGAAATCCG ACAAGAGAAA AAAAGACAAA GAAAAAAAAG AAAAGAAGAA GAAAGCAAAA 
AAGGCCTCAA CCAAAGATTC TGAGTCACCG TCCCAGAAGA AAAAGAAGAA AAAGAAGAAG 
ACAGCAGAGC AGACTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011535980.1
CDS	48..4448
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_011537678.3
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011537678.3

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTGTCA 
AAGAAGAACC CAGCTTCCCT CCCACTGACC CAGGCTGCCC TGAAGGTCCT CGCCCAGAAA 
GCCAGTGAGG CTCAGCCTCC TGTTGCCAGG ACCCAGCCTT CAAGTGGGGT TGACAGTGCT 
GTGGGAACAC TCCCTGCAAC AAGTCCCCAG AGCACCTCCG TCCAGGCCAA AGGGACCAAC 
AAGCTCAGAA AACCTAAGCT TCCTGAGGTC CAGCAGGCCA CCAAAGCCCC TGAGAGCTCA 
GATGACAGTG AGGACAGCAG CGACAGTTCT TCAGGGAGTG AGGAAGATGG TGAAGGGCCC 
CAGGGGGCCA AGTCAGCCCA CACGCTGGTA GGTCCCACCC CCTCCAGGAC AGAGACCCTG 
GTGGAGGAGA CCGCAGCAGA GTCCAGCGAG GATGATGTGG TGGCGCCATC CCAGTCTCTC 
CTCTCAGGTT ATATGACCCC TGGACTAACC CCAGCCAATT CCCAGGCCTC AAAAGCCACT 
CCCAAGCTAG ACTCCAGCCC CTCAGTTTCC TCTACTCTGG CCGCCAAAGA TGACCCAGAT 
GGCAAGCAGG AGGCAAAGCC CCAACAGGCA GCAGGCATGT TGTCCCCTAA AACAGGTGGA 
AAAGAGGCTG CTTCAGGCAC CACACCTCAG AAGTCCCGGA AGCCCAAGAA AGGGGCTGGG 
AACCCCCAAG CCTCAACCCT GGCGCTGCAA AGCAACATCA CCCAGTGCCT CCTGGGCCAA 
CCCTGGCCCC TGAATGAGGC CCAGGTGCAG GCCTCAGTGG TGAAGGTCCT GACTGAGCTG 
CTGGAACAGG AAAGAAAGAA GGTGGTGGAC ACCACCAAGG AGAGCAGCAG GAAGGGCTGG 
GAGAGCCGCA AGCGGAAGCT ATCGGGAGAC CAGCCAGCTG CCAGGACCCC CAGGAGCAAG 
AAGAAGAAGA AGCTGGGGGC CGGGGAAGGT GGGGAGGCCT CTGTTTCCCC AGAAAAGACC 
TCCACGACTT CCAAGGGGAA AGCAAAGAGA GACAAAGCAA GTGGTGATGT CAAGGAGAAG 
AAAGGGAAGG GGTCTCTTGG CTCCCAAGGG GCCAAGGACG AGCCAGAAGA GGAGCTTCAG 
AAGGGGATGG GGACGGTTGA AGGTGGAGAT CAAAGCAACC CAAAGAGCAA GAAGGAGAAG 
AAGAAATCCG ACAAGAGAAA AAAAGACAAA GAAAAAAAAG AAAAGAAGAA GAAAGCAAAA 
AAGGCCTCAA CCAAAGATTC TGAGTCACCG TCCCAGAAGA AAAAGAAGAA AAAGAAGAAG 
ACAGCAGAGC AGACTGTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_017009792.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865281.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OHu96032
Clone ID Related Accession (Same CDS sequence)	XM_017009792.1 , XM_017009792.2
Accession Version	XM_017009792.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTTGAC 
AGTGCTGTGG GAACACTCCC TGCAACAAGT CCCCAGAGCA CCTCCGTCCA GGCCAAAGGG 
ACCAACAAGC TCAGAAAACC TAAGCTTCCT GAGGTCCAGC AGGCCACCAA AGCCCCTGAG 
AGCTCAGATG ACAGTGAGGA CAGCAGCGAC AGTTCTTCAG GGAGTGAGGA AGATGGTGAA 
GGGCCCCAGG GGGCCAAGTC AGCCCACACG CTGGGTCCCA CCCCCTCCAG GACAGAGACC 
CTGGTGGAGG AGACCGCAGC AGAGTCCAGC GAGGATGATG TGGTGGCGCC ATCCCAGTCT 
CTCCTCTCAG GTTATATGAC CCCTGGACTA ACCCCAGCCA ATTCCCAGGC CTCAAAAGCC 
ACTCCCAAGC TAGACTCCAG CCCCTCAGTT TCCTCTACTC TGGCCGCCAA AGATGACCCA 
GATGGCAAGC AGGAGGCAAA GCCCCAACAG GCAGCAGGCA TGTTGTCCCC TAAAACAGGT 
GGAAAAGAGG CTGCTTCAGG CACCACACCT CAGAAGTCCC GGAAGCCCAA GAAAGGGGCT 
GGGAACCCCC AAGCCTCAAC CCTGGCGCTG CAAAGCAACA TCACCCAGTG CCTCCTGGGC 
CAACCCTGGC CCCTGAATGA GGCCCAGGTG CAGGCCTCAG TGGTGAAGGT CCTGACTGAG 
CTGCTGGAAC AGGAAAGAAA GAAGGTGGTG GACACCACCA AGGAGAGCAG CAGGAAGGGC 
TGGGAGAGCC GCAAGCGGAA GCTATCGGGA GACCAGCCAG CTGCCAGGAC CCCCAGGAGC 
AAGAAGAAGA AGAAGCTGGG GGCCGGGGAA GGTGGGGAGG CCTCTGTTTC CCCAGAAAAG 
ACCTCCACGA CTTCCAAGGG GAAAGCAAAG AGAGACAAAG CAAGTGGTGA TGTCAAGGAG 
AAGAAAGGGA AGGGGTCTCT TGGCTCCCAA GGGGCCAAGG ACGAGCCAGA AGAGGAGCTT 
CAGAAGGGGA TGGGGACGGT TGAAGGTGGA GATCAAAGCA ACCCAAAGAG CAAGAAGGAG 
AAGAAGAAAT CCGACAAGAG AAAAAAAGAC AAAGAAAAAA AAGAAAAGAA GAAGAAAGCA 
AAAAAGGCCT CAACCAAAGA TTCTGAGTCA CCGTCCCAGA AGAAAAAGAA GAAAAAGAAG 
AAGACAGCAG AGCAGACTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865281.1
CDS	46..4509
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_017009792.1
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009792.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTTGAC 
AGTGCTGTGG GAACACTCCC TGCAACAAGT CCCCAGAGCA CCTCCGTCCA GGCCAAAGGG 
ACCAACAAGC TCAGAAAACC TAAGCTTCCT GAGGTCCAGC AGGCCACCAA AGCCCCTGAG 
AGCTCAGATG ACAGTGAGGA CAGCAGCGAC AGTTCTTCAG GGAGTGAGGA AGATGGTGAA 
GGGCCCCAGG GGGCCAAGTC AGCCCACACG CTGGGTCCCA CCCCCTCCAG GACAGAGACC 
CTGGTGGAGG AGACCGCAGC AGAGTCCAGC GAGGATGATG TGGTGGCGCC ATCCCAGTCT 
CTCCTCTCAG GTTATATGAC CCCTGGACTA ACCCCAGCCA ATTCCCAGGC CTCAAAAGCC 
ACTCCCAAGC TAGACTCCAG CCCCTCAGTT TCCTCTACTC TGGCCGCCAA AGATGACCCA 
GATGGCAAGC AGGAGGCAAA GCCCCAACAG GCAGCAGGCA TGTTGTCCCC TAAAACAGGT 
GGAAAAGAGG CTGCTTCAGG CACCACACCT CAGAAGTCCC GGAAGCCCAA GAAAGGGGCT 
GGGAACCCCC AAGCCTCAAC CCTGGCGCTG CAAAGCAACA TCACCCAGTG CCTCCTGGGC 
CAACCCTGGC CCCTGAATGA GGCCCAGGTG CAGGCCTCAG TGGTGAAGGT CCTGACTGAG 
CTGCTGGAAC AGGAAAGAAA GAAGGTGGTG GACACCACCA AGGAGAGCAG CAGGAAGGGC 
TGGGAGAGCC GCAAGCGGAA GCTATCGGGA GACCAGCCAG CTGCCAGGAC CCCCAGGAGC 
AAGAAGAAGA AGAAGCTGGG GGCCGGGGAA GGTGGGGAGG CCTCTGTTTC CCCAGAAAAG 
ACCTCCACGA CTTCCAAGGG GAAAGCAAAG AGAGACAAAG CAAGTGGTGA TGTCAAGGAG 
AAGAAAGGGA AGGGGTCTCT TGGCTCCCAA GGGGCCAAGG ACGAGCCAGA AGAGGAGCTT 
CAGAAGGGGA TGGGGACGGT TGAAGGTGGA GATCAAAGCA ACCCAAAGAG CAAGAAGGAG 
AAGAAGAAAT CCGACAAGAG AAAAAAAGAC AAAGAAAAAA AAGAAAAGAA GAAGAAAGCA 
AAAAAGGCCT CAACCAAAGA TTCTGAGTCA CCGTCCCAGA AGAAAAAGAA GAAAAAGAAG 
AAGACAGCAG AGCAGACTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_017009792.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865281.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.

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CloneID	OHu96032
Clone ID Related Accession (Same CDS sequence)	XM_017009792.1 , XM_017009792.2
Accession Version	XM_017009792.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4464bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTTGAC 
AGTGCTGTGG GAACACTCCC TGCAACAAGT CCCCAGAGCA CCTCCGTCCA GGCCAAAGGG 
ACCAACAAGC TCAGAAAACC TAAGCTTCCT GAGGTCCAGC AGGCCACCAA AGCCCCTGAG 
AGCTCAGATG ACAGTGAGGA CAGCAGCGAC AGTTCTTCAG GGAGTGAGGA AGATGGTGAA 
GGGCCCCAGG GGGCCAAGTC AGCCCACACG CTGGGTCCCA CCCCCTCCAG GACAGAGACC 
CTGGTGGAGG AGACCGCAGC AGAGTCCAGC GAGGATGATG TGGTGGCGCC ATCCCAGTCT 
CTCCTCTCAG GTTATATGAC CCCTGGACTA ACCCCAGCCA ATTCCCAGGC CTCAAAAGCC 
ACTCCCAAGC TAGACTCCAG CCCCTCAGTT TCCTCTACTC TGGCCGCCAA AGATGACCCA 
GATGGCAAGC AGGAGGCAAA GCCCCAACAG GCAGCAGGCA TGTTGTCCCC TAAAACAGGT 
GGAAAAGAGG CTGCTTCAGG CACCACACCT CAGAAGTCCC GGAAGCCCAA GAAAGGGGCT 
GGGAACCCCC AAGCCTCAAC CCTGGCGCTG CAAAGCAACA TCACCCAGTG CCTCCTGGGC 
CAACCCTGGC CCCTGAATGA GGCCCAGGTG CAGGCCTCAG TGGTGAAGGT CCTGACTGAG 
CTGCTGGAAC AGGAAAGAAA GAAGGTGGTG GACACCACCA AGGAGAGCAG CAGGAAGGGC 
TGGGAGAGCC GCAAGCGGAA GCTATCGGGA GACCAGCCAG CTGCCAGGAC CCCCAGGAGC 
AAGAAGAAGA AGAAGCTGGG GGCCGGGGAA GGTGGGGAGG CCTCTGTTTC CCCAGAAAAG 
ACCTCCACGA CTTCCAAGGG GAAAGCAAAG AGAGACAAAG CAAGTGGTGA TGTCAAGGAG 
AAGAAAGGGA AGGGGTCTCT TGGCTCCCAA GGGGCCAAGG ACGAGCCAGA AGAGGAGCTT 
CAGAAGGGGA TGGGGACGGT TGAAGGTGGA GATCAAAGCA ACCCAAAGAG CAAGAAGGAG 
AAGAAGAAAT CCGACAAGAG AAAAAAAGAC AAAGAAAAAA AAGAAAAGAA GAAGAAAGCA 
AAAAAGGCCT CAACCAAAGA TTCTGAGTCA CCGTCCCAGA AGAAAAAGAA GAAAAAGAAG 
AAGACAGCAG AGCAGACTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865281.1
CDS	48..4511
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQKDSNSKPARSKTLAPAPPERNTEGSSESSEEELPLTQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_017009792.2
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009792.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCACCA GTGAGAAACC CCCAGAACAG TACCGTCTTG 
GCGAGGGGCC CAGCATCTGT GCCATCTGTG GGGAAGGCCG TGGCTACAGC AGCTCAGGCC 
CAGACAGGGC CAGAGGAGGA CTCAGGGAGC AGTGAGGAGG AGTCAGACAG TGAGGAGGAG 
GCGGAGACGC TGGCTCAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGA AGGACAGTAA CTCCAAACCT 
GCCAGAAGCA AGACCCTGGC CCCAGCACCC CCAGAGAGGA ACACGGAGGG GTCCTCGGAG 
AGCAGTGAGG AAGAGCTGCC ACTGACCCAG GTGATTAAAC CCCCTCTGAT TTTTGTCGAC 
CCTAATCGTA GTCCAGCTGG CCCAGCTGCT ACACCCGCAC AAGCCCAGGC TGCAAGCACC 
CCGAGGAAGG CCCGAGCCTC GGAGAGCACA GCCAGGAGCT CCTCCTCCGA GAGCGAGGAT 
GAGGACGTGA TCCCCGCTAC ACAGTGCTTG ACTCCTGGCA TCAGAACCAA TGTGGTGACC 
ATGCCCACTG CCCACCCAAG AATAGCCCCC AAAGCCAGCA TGGCTGGGGC CAGCAGCAGC 
AAGGAGTCCA GTCGGATATC AGATGGCAAG AAACAGGAGG GACCAGCCAC TCAGGTTGAC 
AGTGCTGTGG GAACACTCCC TGCAACAAGT CCCCAGAGCA CCTCCGTCCA GGCCAAAGGG 
ACCAACAAGC TCAGAAAACC TAAGCTTCCT GAGGTCCAGC AGGCCACCAA AGCCCCTGAG 
AGCTCAGATG ACAGTGAGGA CAGCAGCGAC AGTTCTTCAG GGAGTGAGGA AGATGGTGAA 
GGGCCCCAGG GGGCCAAGTC AGCCCACACG CTGGGTCCCA CCCCCTCCAG GACAGAGACC 
CTGGTGGAGG AGACCGCAGC AGAGTCCAGC GAGGATGATG TGGTGGCGCC ATCCCAGTCT 
CTCCTCTCAG GTTATATGAC CCCTGGACTA ACCCCAGCCA ATTCCCAGGC CTCAAAAGCC 
ACTCCCAAGC TAGACTCCAG CCCCTCAGTT TCCTCTACTC TGGCCGCCAA AGATGACCCA 
GATGGCAAGC AGGAGGCAAA GCCCCAACAG GCAGCAGGCA TGTTGTCCCC TAAAACAGGT 
GGAAAAGAGG CTGCTTCAGG CACCACACCT CAGAAGTCCC GGAAGCCCAA GAAAGGGGCT 
GGGAACCCCC AAGCCTCAAC CCTGGCGCTG CAAAGCAACA TCACCCAGTG CCTCCTGGGC 
CAACCCTGGC CCCTGAATGA GGCCCAGGTG CAGGCCTCAG TGGTGAAGGT CCTGACTGAG 
CTGCTGGAAC AGGAAAGAAA GAAGGTGGTG GACACCACCA AGGAGAGCAG CAGGAAGGGC 
TGGGAGAGCC GCAAGCGGAA GCTATCGGGA GACCAGCCAG CTGCCAGGAC CCCCAGGAGC 
AAGAAGAAGA AGAAGCTGGG GGCCGGGGAA GGTGGGGAGG CCTCTGTTTC CCCAGAAAAG 
ACCTCCACGA CTTCCAAGGG GAAAGCAAAG AGAGACAAAG CAAGTGGTGA TGTCAAGGAG 
AAGAAAGGGA AGGGGTCTCT TGGCTCCCAA GGGGCCAAGG ACGAGCCAGA AGAGGAGCTT 
CAGAAGGGGA TGGGGACGGT TGAAGGTGGA GATCAAAGCA ACCCAAAGAG CAAGAAGGAG 
AAGAAGAAAT CCGACAAGAG AAAAAAAGAC AAAGAAAAAA AAGAAAAGAA GAAGAAAGCA 
AAAAAGGCCT CAACCAAAGA TTCTGAGTCA CCGTCCCAGA AGAAAAAGAA GAAAAAGAAG 
AAGACAGCAG AGCAGACTGT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_017009793.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865282.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X9, mRNA.

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CloneID	OHu96033
Clone ID Related Accession (Same CDS sequence)	XM_017009793.2 , XM_017009793.1
Accession Version	XM_017009793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865282.1
CDS	48..4337
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_017009793.2
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009793.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_017009793.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865282.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu96033
Clone ID Related Accession (Same CDS sequence)	XM_017009793.2 , XM_017009793.1
Accession Version	XM_017009793.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4290bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865282.1
CDS	46..4335
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVSKKNPASLPLTQAALKVLAQKASEAQPPVARTQPSSGVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_017009793.1
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009793.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTGTCAA AGAAGAACCC AGCTTCCCTC CCACTGACCC AGGCTGCCCT GAAGGTCCTC 
GCCCAGAAAG CCAGTGAGGC TCAGCCTCCT GTTGCCAGGA CCCAGCCTTC AAGTGGGGTT 
GACAGTGCTG TGGGAACACT CCCTGCAACA AGTCCCCAGA GCACCTCCGT CCAGGCCAAA 
GGGACCAACA AGCTCAGAAA ACCTAAGCTT CCTGAGGTCC AGCAGGCCAC CAAAGCCCCT 
GAGAGCTCAG ATGACAGTGA GGACAGCAGC GACAGTTCTT CAGGGAGTGA GGAAGATGGT 
GAAGGGCCCC AGGGGGCCAA GTCAGCCCAC ACGCTGGTAG GTCCCACCCC CTCCAGGACA 
GAGACCCTGG TGGAGGAGAC CGCAGCAGAG TCCAGCGAGG ATGATGTGGT GGCGCCATCC 
CAGTCTCTCC TCTCAGGTTA TATGACCCCT GGACTAACCC CAGCCAATTC CCAGGCCTCA 
AAAGCCACTC CCAAGCTAGA CTCCAGCCCC TCAGTTTCCT CTACTCTGGC CGCCAAAGAT 
GACCCAGATG GCAAGCAGGA GGCAAAGCCC CAACAGGCAG CAGGCATGTT GTCCCCTAAA 
ACAGGTGGAA AAGAGGCTGC TTCAGGCACC ACACCTCAGA AGTCCCGGAA GCCCAAGAAA 
GGGGCTGGGA ACCCCCAAGC CTCAACCCTG GCGCTGCAAA GCAACATCAC CCAGTGCCTC 
CTGGGCCAAC CCTGGCCCCT GAATGAGGCC CAGGTGCAGG CCTCAGTGGT GAAGGTCCTG 
ACTGAGCTGC TGGAACAGGA AAGAAAGAAG GTGGTGGACA CCACCAAGGA GAGCAGCAGG 
AAGGGCTGGG AGAGCCGCAA GCGGAAGCTA TCGGGAGACC AGCCAGCTGC CAGGACCCCC 
AGGAGCAAGA AGAAGAAGAA GCTGGGGGCC GGGGAAGGTG GGGAGGCCTC TGTTTCCCCA 
GAAAAGACCT CCACGACTTC CAAGGGGAAA GCAAAGAGAG ACAAAGCAAG TGGTGATGTC 
AAGGAGAAGA AAGGGAAGGG GTCTCTTGGC TCCCAAGGGG CCAAGGACGA GCCAGAAGAG 
GAGCTTCAGA AGGGGATGGG GACGGTTGAA GGTGGAGATC AAAGCAACCC AAAGAGCAAG 
AAGGAGAAGA AGAAATCCGA CAAGAGAAAA AAAGACAAAG AAAAAAAAGA AAAGAAGAAG 
AAAGCAAAAA AGGCCTCAAC CAAAGATTCT GAGTCACCGT CCCAGAAGAA AAAGAAGAAA 
AAGAAGAAGA CAGCAGAGCA GACTGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_017009794.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865283.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu96034
Clone ID Related Accession (Same CDS sequence)	XM_017009794.2 , XM_017009794.1
Accession Version	XM_017009794.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009794.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAC CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGGTGAAG GGCCCCAGGG GGCCAAGTCA GCCCACACGC TGGTAGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCACCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGG AAGCTATCGG GAGACCAGCC AGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCGGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCAAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGACG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865283.1
CDS	48..4223
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_017009794.2
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009794.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAC CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGGTGAAG GGCCCCAGGG GGCCAAGTCA GCCCACACGC TGGTAGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCACCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGG AAGCTATCGG GAGACCAGCC AGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCGGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCAAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGACG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_017009794.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865283.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X10, mRNA.

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CloneID	OHu96034
Clone ID Related Accession (Same CDS sequence)	XM_017009794.2 , XM_017009794.1
Accession Version	XM_017009794.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4176bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAC CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGGTGAAG GGCCCCAGGG GGCCAAGTCA GCCCACACGC TGGTAGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCACCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGG AAGCTATCGG GAGACCAGCC AGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCGGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCAAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGACG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865283.1
CDS	46..4221
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQVIKPPLIFVDPNRSPAGPAATPAQAQAASTPRKARASESTARSSSSESEDEDVIPATQCLTPGIRTNVVTMPTAHPRIAPKASMAGASSSKESSRISDGKKQEGPATQVDSAVGTLPATSPQSTSVQAKGTNKLRKPKLPEVQQATKAPESSDDSEDSSDSSSGSEEDGEGPQGAKSAHTLVGPTPSRTETLVEETAAESSEDDVVAPSQSLLSGYMTPGLTPANSQASKATPKLDSSPSVSSTLAAKDDPDGKQEAKPQQAAGMLSPKTGGKEAASGTTPQKSRKPKKGAGNPQASTLALQSNITQCLLGQPWPLNEAQVQASVVKVLTELLEQERKKVVDTTKESSRKGWESRKRKLSGDQPAARTPRSKKKKKLGAGEGGEASVSPEKTSTTSKGKAKRDKASGDVKEKKGKGSLGSQGAKDEPEEELQKGMGTVEGGDQSNPKSKKEKKKSDKRKKDKEKKEKKKKAKKASTKDSESPSQKKKKKKKKTAEQTV

Target ORF information:

RefSeq Version	XM_017009794.1
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009794.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG GGAAACCCTC AGTAAAACCA 
GCCCAGGTCA AAGCCTCATC AGTTTCTACT AAGGAGTCTC CAGCAAGAAA GGCGGCCCCA 
GCCCCTGGGA AGGTGGGGGA TGTGACACCC CAGGTCAAAG GAGGGGCCCT GCCCCCAGCC 
AAGAGGGCCA AGAAGCCAGA AGAGGAGTCA GAGAGTAGTG AGGAGGGATC TGAAAGTGAG 
GAGGAGGCCC CTGCAGGGAC ACGAAGCCAG GTAAAGGCCT CTGAAAAAAT TCTCCAGGTC 
AGAGCTGCCT CAGCCCCTGC CAAGGGGACC CCTGGGAAAG GGGCTACCCC AGCACCCCCT 
GGGAAGGCAG GGGCTGTAGC CTCCCAGACC AAGGCAGGGA AGCCAGAGGA GGACTCAGAG 
AGCAGCAGCG AGGAGTCATC TGACAGTGAG GAGGAGACGC CAGCTGCCAA GGCCCTGCTT 
CAGGCGAAGG CCTCAGGAAA AACCTCTCAG GTCGGAGCTG CCTCAGCCCC TGCCAAGGAG 
TCCCCCAGGA AAGGAGCTGC CCCAGCGCCC CCTGGGAAGA CAGGGCCTGC AGTTGCCAAG 
GCCCAGGCGG GGAAGCGGGA GGAGGACTCG CAGAGCAGCA GCGAGGAATC GGACAGTGAG 
GAGGAGGCGC CTGCTCAGGC GAAGCCTTCA GGGAAGGCCC CCCAGGTCAG AGCCGCCTCG 
GCCCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAG CACCTCCTAG GAAAACAGGG 
CCTGCAGCCG CCCAGGTCCA GGTGGGGAAG CAGGAGGAGG ACTCAAGAAG CAGCAGCGAG 
GAGTCAGACA GTGACAGAGA GGCACTGGCA GCCATGAATG CAGCTCAGGT GAAGCCCTTG 
GGGAAAAGCC CCCAGGTGAA ACCTGCCTCT ACCATGGGCA TGGGGCCCTT GGGGAAAGGC 
GCCGGCCCAG TGCCACCCGG GAAGGTGGGG CCTGCAACCC CCTCAGCCCA GGTGGGGAAG 
TGGGAGGAGG ACTCAGAGAG CAGTAGTGAG GAGTCATCAG ACAGCAGTGA TGGAGAGGTG 
CCCACAGCTG TGGCCCCGGC TCAGGAAAAG TCCTTGGGGA ACATCCTCCA GGCCAAACCC 
ACCTCCAGTC CTGCCAAGGG GCCCCCTCAG AAGGCAGGGC CTGTAGCCGT CCAGGTCAAG 
GCTGAAAAGC CCATGGACAA CTCGGAGAGC AGCGAGGAGT CATCGGACAG TGCGGACAGT 
GAGGAGGCAC CAGCAGCCAT GACTGCAGCT CAGGCAAAAC CAGCTCTGAA AATTCCTCAG 
ACCAAGGCCT GCCCAAAGAA AACCAATACC ACTGCATCTG CCAAGGTCGC CCCTGTGCGA 
GTGGGCACCC AAGCCCCCCG GAAAGCAGGA ACTGCGACTT CTCCAGCAGG CTCATCCCCA 
GCTGTGGCTG GGGGCACCCA GAGACCAGCA GAGGATTCTT CAAGCAGTGA GGAATCAGAT 
AGTGAGGAAG AGAAGACAGG TCTTGCAGTA ACCGTGGGAC AGGCAAAGTC TGTGGGGAAA 
GGCCTCCAGG TGAAAGCAGC CTCAGTGCCT GTCAAGGGGT CCTTGGGGCA AGGGACTGCT 
CCAGTACTCC CTGGGAAGAC GGGGCCTACA GTCACCCAGG TGAAAGCTGA AAAGCAGGAA 
GACTCTGAGA GCAGTGAGGA GGAATCAGAC AGTGAGGAAG CAGCTGCATC TCCAGCACAG 
GTGAAAACCT CAGTAAAGAA AACCCAGGCC AAAGCCAACC CAGCTGCCGC CAGAGCACCT 
TCAGCAAAAG GGACAATTTC AGCCCCTGGA AAAGTTGTCA CTGCAGCTGC TCAAGCCAAG 
CAGAGGTCTC CATCCAAGGT GAAGCCTTCA GGGAAGACCC ACCAGATCAG AGCTGCCTTG 
GCTCCTGCCA AGGAGTCCCC CAGGAAAGGG GCTGCCCCAA CACCTCCTGG GAAGACAGGG 
CCTTCGGCTG CCCAGGCAGG GAAGCAGGAT GACTCAGGGA GCAGCAGCGA GGAATCAGAC 
AGTGATGGGG AGGCACCGGC AGCTGTGACC TCTGCCCAGG TGATTAAACC CCCTCTGATT 
TTTGTCGACC CTAATCGTAG TCCAGCTGGC CCAGCTGCTA CACCCGCACA AGCCCAGGCT 
GCAAGCACCC CGAGGAAGGC CCGAGCCTCG GAGAGCACAG CCAGGAGCTC CTCCTCCGAG 
AGCGAGGATG AGGACGTGAT CCCCGCTACA CAGTGCTTGA CTCCTGGCAT CAGAACCAAT 
GTGGTGACCA TGCCCACTGC CCACCCAAGA ATAGCCCCCA AAGCCAGCAT GGCTGGGGCC 
AGCAGCAGCA AGGAGTCCAG TCGGATATCA GATGGCAAGA AACAGGAGGG ACCAGCCACT 
CAGGTTGACA GTGCTGTGGG AACACTCCCT GCAACAAGTC CCCAGAGCAC CTCCGTCCAG 
GCCAAAGGGA CCAACAAGCT CAGAAAACCT AAGCTTCCTG AGGTCCAGCA GGCCACCAAA 
GCCCCTGAGA GCTCAGATGA CAGTGAGGAC AGCAGCGACA GTTCTTCAGG GAGTGAGGAA 
GATGGTGAAG GGCCCCAGGG GGCCAAGTCA GCCCACACGC TGGTAGGTCC CACCCCCTCC 
AGGACAGAGA CCCTGGTGGA GGAGACCGCA GCAGAGTCCA GCGAGGATGA TGTGGTGGCG 
CCATCCCAGT CTCTCCTCTC AGGTTATATG ACCCCTGGAC TAACCCCAGC CAATTCCCAG 
GCCTCAAAAG CCACTCCCAA GCTAGACTCC AGCCCCTCAG TTTCCTCTAC TCTGGCCGCC 
AAAGATGACC CAGATGGCAA GCAGGAGGCA AAGCCCCAAC AGGCAGCAGG CATGTTGTCC 
CCTAAAACAG GTGGAAAAGA GGCTGCTTCA GGCACCACAC CTCAGAAGTC CCGGAAGCCC 
AAGAAAGGGG CTGGGAACCC CCAAGCCTCA ACCCTGGCGC TGCAAAGCAA CATCACCCAG 
TGCCTCCTGG GCCAACCCTG GCCCCTGAAT GAGGCCCAGG TGCAGGCCTC AGTGGTGAAG 
GTCCTGACTG AGCTGCTGGA ACAGGAAAGA AAGAAGGTGG TGGACACCAC CAAGGAGAGC 
AGCAGGAAGG GCTGGGAGAG CCGCAAGCGG AAGCTATCGG GAGACCAGCC AGCTGCCAGG 
ACCCCCAGGA GCAAGAAGAA GAAGAAGCTG GGGGCCGGGG AAGGTGGGGA GGCCTCTGTT 
TCCCCAGAAA AGACCTCCAC GACTTCCAAG GGGAAAGCAA AGAGAGACAA AGCAAGTGGT 
GATGTCAAGG AGAAGAAAGG GAAGGGGTCT CTTGGCTCCC AAGGGGCCAA GGACGAGCCA 
GAAGAGGAGC TTCAGAAGGG GATGGGGACG GTTGAAGGTG GAGATCAAAG CAACCCAAAG 
AGCAAGAAGG AGAAGAAGAA ATCCGACAAG AGAAAAAAAG ACAAAGAAAA AAAAGAAAAG 
AAGAAGAAAG CAAAAAAGGC CTCAACCAAA GATTCTGAGT CACCGTCCCA GAAGAAAAAG 
AAGAAAAAGA AGAAGACAGC AGAGCAGACT GTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_017009795.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865284.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu96035
Clone ID Related Accession (Same CDS sequence)	XM_017009795.2 , XM_017009795.1
Accession Version	XM_017009795.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017009795.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGGA CCAGGAGTCT 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865284.1
CDS	48..2693
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQDQESS

Target ORF information:

RefSeq Version	XM_017009795.2
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009795.2

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGGA CCAGGAGTCT 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TCOF1 ( XM_017009795.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016865284.1 Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu96035
Clone ID Related Accession (Same CDS sequence)	XM_017009795.2 , XM_017009795.1
Accession Version	XM_017009795.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2646bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	treacle protein isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGGA CCAGGAGTCT 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016865284.1
CDS	46..2691
Translation	MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSEEETPAAKALLQAKASGKTSQVGAASAPAKESPRKGAAPAPPGKTGPAVAKAQAGKREEDSQSSSEESDSEEEAPAQAKPSGKAPQVRAASAPAKESPRKGAAPAPPRKTGPAAAQVQVGKQEEDSRSSSEESDSDREALAAMNAAQVKPLGKSPQVKPASTMGMGPLGKGAGPVPPGKVGPATPSAQVGKWEEDSESSSEESSDSSDGEVPTAVAPAQEKSLGNILQAKPTSSPAKGPPQKAGPVAVQVKAEKPMDNSESSEESSDSADSEEAPAAMTAAQAKPALKIPQTKACPKKTNTTASAKVAPVRVGTQAPRKAGTATSPAGSSPAVAGGTQRPAEDSSSSEESDSEEEKTGLAVTVGQAKSVGKGLQVKAASVPVKGSLGQGTAPVLPGKTGPTVTQVKAEKQEDSESSEEESDSEEAAASPAQVKTSVKKTQAKANPAAARAPSAKGTISAPGKVVTAAAQAKQRSPSKVKPPVRNPQNSTVLARGPASVPSVGKAVATAAQAQTGPEEDSGSSEEESDSEEEAETLAQVKPSGKTHQIRAALAPAKESPRKGAAPTPPGKTGPSAAQAGKQDDSGSSSEESDSDGEAPAAVTSAQDQESS

Target ORF information:

RefSeq Version	XM_017009795.1
Organism	Homo sapiens(human)
Definition	Homo sapiens treacle ribosome biogenesis factor 1 (TCOF1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017009795.1

ORF Insert Sequence:

ATGGCCGAGG CCAGGAAGCG GCGGGAGCTA CTTCCCCTGA TCTACCACCA TCTGCTGCGG 
GCTGGCTATG TGCGTGCGGC GCGGGAAGTG AAGGAGCAGA GCGGCCAGAA GTGTTTCCTG 
GCTCAGCCCG TAACCCTTCT GGACATCTAT ACACACTGGC AACAAACCTC AGAGCTTGGT 
CGGAAGCGGA AGGCAGAGGA AGATGCGGCA CTGCAAGCTA AGAAAACCCG TGTGTCAGAC 
CCCATCAGCA CCTCGGAGAG CTCGGAAGAG GAGGAAGAAG CAGAAGCCGA AACCGCCAAA 
GCCACCCCAA GACTAGCATC TACCAACTCC TCAGTCCTGG GGGCGGACTT GCCATCAAGC 
ATGAAAGAAA AAGCCAAGGC AGAGACAGAG AAAGCTGGCA AGACTGGGAA TTCCATGCCA 
CACCCTGCCA CTGGGAAGAC GGTGGCCAAC CTTCTTTCTG GGAAGTCTCC CAGGAAGTCA 
GCAGAGCCCT CAGCAAATAC TACGTTGGTC TCAGAAACTG AGGAGGAGGG CAGCGTCCCG 
GCCTTTGGAG CTGCTGCCAA GCCTGGGATG GTGTCAGCGG GCCAGGCCGA CAGCTCCAGC 
GAGGACACCT CCAGCTCCAG TGATGAGACA GACGTGGAGG TAAAGGCCTC TGAAAAAATT 
CTCCAGGTCA GAGCTGCCTC AGCCCCTGCC AAGGGGACCC CTGGGAAAGG GGCTACCCCA 
GCACCCCCTG GGAAGGCAGG GGCTGTAGCC TCCCAGACCA AGGCAGGGAA GCCAGAGGAG 
GACTCAGAGA GCAGCAGCGA GGAGTCATCT GACAGTGAGG AGGAGACGCC AGCTGCCAAG 
GCCCTGCTTC AGGCGAAGGC CTCAGGAAAA ACCTCTCAGG TCGGAGCTGC CTCAGCCCCT 
GCCAAGGAGT CCCCCAGGAA AGGAGCTGCC CCAGCGCCCC CTGGGAAGAC AGGGCCTGCA 
GTTGCCAAGG CCCAGGCGGG GAAGCGGGAG GAGGACTCGC AGAGCAGCAG CGAGGAATCG 
GACAGTGAGG AGGAGGCGCC TGCTCAGGCG AAGCCTTCAG GGAAGGCCCC CCAGGTCAGA 
GCCGCCTCGG CCCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAGC ACCTCCTAGG 
AAAACAGGGC CTGCAGCCGC CCAGGTCCAG GTGGGGAAGC AGGAGGAGGA CTCAAGAAGC 
AGCAGCGAGG AGTCAGACAG TGACAGAGAG GCACTGGCAG CCATGAATGC AGCTCAGGTG 
AAGCCCTTGG GGAAAAGCCC CCAGGTGAAA CCTGCCTCTA CCATGGGCAT GGGGCCCTTG 
GGGAAAGGCG CCGGCCCAGT GCCACCCGGG AAGGTGGGGC CTGCAACCCC CTCAGCCCAG 
GTGGGGAAGT GGGAGGAGGA CTCAGAGAGC AGTAGTGAGG AGTCATCAGA CAGCAGTGAT 
GGAGAGGTGC CCACAGCTGT GGCCCCGGCT CAGGAAAAGT CCTTGGGGAA CATCCTCCAG 
GCCAAACCCA CCTCCAGTCC TGCCAAGGGG CCCCCTCAGA AGGCAGGGCC TGTAGCCGTC 
CAGGTCAAGG CTGAAAAGCC CATGGACAAC TCGGAGAGCA GCGAGGAGTC ATCGGACAGT 
GCGGACAGTG AGGAGGCACC AGCAGCCATG ACTGCAGCTC AGGCAAAACC AGCTCTGAAA 
ATTCCTCAGA CCAAGGCCTG CCCAAAGAAA ACCAATACCA CTGCATCTGC CAAGGTCGCC 
CCTGTGCGAG TGGGCACCCA AGCCCCCCGG AAAGCAGGAA CTGCGACTTC TCCAGCAGGC 
TCATCCCCAG CTGTGGCTGG GGGCACCCAG AGACCAGCAG AGGATTCTTC AAGCAGTGAG 
GAATCAGATA GTGAGGAAGA GAAGACAGGT CTTGCAGTAA CCGTGGGACA GGCAAAGTCT 
GTGGGGAAAG GCCTCCAGGT GAAAGCAGCC TCAGTGCCTG TCAAGGGGTC CTTGGGGCAA 
GGGACTGCTC CAGTACTCCC TGGGAAGACG GGGCCTACAG TCACCCAGGT GAAAGCTGAA 
AAGCAGGAAG ACTCTGAGAG CAGTGAGGAG GAATCAGACA GTGAGGAAGC AGCTGCATCT 
CCAGCACAGG TGAAAACCTC AGTAAAGAAA ACCCAGGCCA AAGCCAACCC AGCTGCCGCC 
AGAGCACCTT CAGCAAAAGG GACAATTTCA GCCCCTGGAA AAGTTGTCAC TGCAGCTGCT 
CAAGCCAAGC AGAGGTCTCC ATCCAAGGTG AAGCCACCAG TGAGAAACCC CCAGAACAGT 
ACCGTCTTGG CGAGGGGCCC AGCATCTGTG CCATCTGTGG GGAAGGCCGT GGCTACAGCA 
GCTCAGGCCC AGACAGGGCC AGAGGAGGAC TCAGGGAGCA GTGAGGAGGA GTCAGACAGT 
GAGGAGGAGG CGGAGACGCT GGCTCAGGTG AAGCCTTCAG GGAAGACCCA CCAGATCAGA 
GCTGCCTTGG CTCCTGCCAA GGAGTCCCCC AGGAAAGGGG CTGCCCCAAC ACCTCCTGGG 
AAGACAGGGC CTTCGGCTGC CCAGGCAGGG AAGCAGGATG ACTCAGGGAG CAGCAGCGAG 
GAATCAGACA GTGATGGGGA GGCACCGGCA GCTGTGACCT CTGCCCAGGA CCAGGAGTCT 
TCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Mutation screening of Chinese Treacher Collins syndrome patients identified novel TCOF1 mutations.
Molecular genetics and genomics : MGG(2017 Dec)
Chen Y,Guo L,Li CL,Shan J,Xu HS,Li JY,Sun S,Hao SJ,Jin L,Chai G,Zhang TY

Mutational Analysis of TCOF1, GSC, and HOXA2 in Patients With Treacher Collins Syndrome.
The Journal of craniofacial surgery27(6)e583-6(2016 Sep)
Hao S,Jin L,Wang H,Li C,Zheng F,Ma D,Zhang T

Tcof1-Related Molecular Networks in Treacher Collins Syndrome.
The Journal of craniofacial surgery27(6)1420-6(2016 Sep)
Dai J,Si J,Wang M,Huang L,Fang B,Shi J,Wang X,Shen G

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TCOF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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