VAMP2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VAMP2
Entrez Gene ID	6844
Full Name	vesicle associated membrane protein 2
Synonyms	SYB2,VAMP-2
General protein information	Preferred Names vesicle associated membrane protein 2 Names vesicle-associated membrane protein 2 synaptobrevin 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.1
Summary	The protein encoded by this gene is a member of the vesicle-associated membrane protein (VAMP)/synaptobrevin family. Synaptobrevins/VAMPs, syntaxins, and the 25-kD synaptosomal-associated protein SNAP25 are the main components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. This gene is thought to participate in neurotransmitter release at a step between docking and fusion. The protein forms a stable complex with syntaxin, synaptosomal-associated protein, 25 kD, and synaptotagmin. It also forms a distinct complex with synaptophysin. It is a likely candidate gene for familial infantile myasthenia (FIMG) because of its map location and because it encodes a synaptic vesicle protein of the type that has been implicated in the pathogenesis of FIMG. [provided by RefSeq, Jul 2008].
Disorder MIM:	185881

mRNA and Protein(s)

mRNA	Protein	Name
NM_001330125.1	NP_001317054.1	vesicle-associated membrane protein 2 isoform 2
NM_014232.2	NP_055047.2	vesicle-associated membrane protein 2 isoform 1
NM_014232.3	NP_055047.2	vesicle-associated membrane protein 2 isoform 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	VAMP2	NP_776908.1
Drosophila melanogaster (fruit fly)	n-syb	NP_001261270.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005507	XP_001688670.1
Pan troglodytes (chimpanzee)	VAMP2	XP_001156324.1
Mus musculus (house mouse)	Vamp2	NP_033523.1
Danio rerio (zebrafish)	vamp2	NP_956299.1
	VAMP2	NP_001027992.1
Canis lupus familiaris (dog)	VAMP2	XP_005620068.1
Rattus norvegicus (Norway rat)	Vamp2	NP_036795.1
Gallus gallus (chicken)	VAMP2	XP_422640.2
Xenopus tropicalis (tropical clawed frog)	vamp2	NP_989031.1
Homo sapiens (human)	VAMP2	NP_055047.2

Gene Ontology
Bibliography

Related articles in PubMed

MicroRNA-493-5p promotes apoptosis and suppresses proliferation and invasion in liver cancer cells by targeting VAMP2.
Wang G, Fang X, Han M, Wang X, Huang Q
International journal of molecular medicine41(3)1740-1748(2018 Mar)

?-adrenoceptor activation-increased VAMP-2 and syntaxin-4 in secretory granules are involved in protein secretion of submandibular gland through the PKA/F-actin pathway.
Ding C, Cong X, Zhang Y, Li SL, Wu LL, Yu GY
Bioscience reports(2018 Jan)

?-Synuclein may cross-bridge v-SNARE and acidic phospholipids to facilitate SNARE-dependent vesicle docking.
Lou X, Kim J, Hawk BJ, Shin YK
The Biochemical journal474(12)2039-2049(2017 06)

VAMP2 is implicated in the secretion of antibodies by human plasma cells and can be replaced by other synaptobrevins.
G?mez-Jaramillo L, Romero-Garc?a R, Jim?nez-G?mez G, Riegle L, Ramos-Amaya AB, Brieva JA, Kelly-Worden M, Campos-Caro A
Cellular & molecular immunology(2016 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that A-syn (alpha-synuclein) promotes SNARE-dependent vesicle docking; phosphatidylserine (PS) removal from t-SNARE-bearing vesicles causes A-syn to inhibit vesicle docking; PS removal from v-SNARE-bearing vesicles promotes vesicle docking; the C-terminal 45 residues of A-syn are required for promotion of vesicle docking. (Here, t-SNARE is SNAP-25; v-SNARE is VAMP2.)
Title: ?-Synuclein may cross-bridge v-SNARE and acidic phospholipids to facilitate SNARE-dependent vesicle docking.

A significant interactive two-locus model of STX1A_rs4363087|VAMP2_rs2278637 (presynaptic genes) was observed among SVC variants in all epilepsy cases.
Title: Synergistic association of STX1A and VAMP2 with cryptogenic epilepsy in North Indian population.

VAMP2 is a promising new plasma cell marker
Title: An Immunohistochemical Survey of SNARE Proteins Shows Distinct Patterns of Expression in Hematolymphoid Neoplasia.

The present study addressed for the first time the unique substrate recognition mechanism of LC/F5 substrate cleavage of VAMP-2 by Botulinum Neurotoxin subtype F5.
Title: Mechanism of substrate recognition by the novel Botulinum Neurotoxin subtype F5.

This study showed that decreased Levels of VAMP2 correlate with Duration of Dementia.
Title: Decreased Levels of VAMP2 and Monomeric Alpha-Synuclein Correlate with Duration of Dementia.

The following VAMP2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VAMP2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu06270	NM_014232.3 Latest version!	Homo sapiens vesicle associated membrane protein 2 (VAMP2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu49924	NM_001330125.1 Latest version!	Homo sapiens vesicle associated membrane protein 2 (VAMP2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu06270	NM_014232.2	Homo sapiens vesicle associated membrane protein 2 (VAMP2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VAMP2 ( NM_014232.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_055047.2 Homo sapiens vesicle associated membrane protein 2 (VAMP2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06270
Clone ID Related Accession (Same CDS sequence)	NM_014232.2 , NM_014232.3
Accession Version	NM_014232.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK225777.1, AK021522.1, AC129492.6 and BU625107.1. On Apr 4, 2008 this sequence version replaced NM_014232.1. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225777.1, ERR279830.349.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGCTA CCGCTGCCAC GGCCCCCCCT GCTGCCCCGG CTGGGGAGGG TGGTCCCCCT 
GCACCCCCTC CAAACCTCAC CAGTAACAGG AGACTGCAGC AGACCCAGGC CCAGGTGGAT 
GAGGTGGTGG ACATCATGAG GGTGAACGTG GACAAGGTCC TGGAGCGAGA CCAGAAGCTG 
TCGGAGCTGG ACGACCGTGC AGATGCACTC CAGGCGGGGG CCTCCCAGTT TGAAACAAGC 
GCAGCCAAGC TCAAGCGCAA ATACTGGTGG AAAAACCTCA AGATGATGAT CATCTTGGGA 
GTGATTTGCG CCATCATCCT CATCATCATC ATAGTTTACT TCAGCACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055047.2
CDS	97..447
Translation	MSATAATAPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIVYFST

Target ORF information:

RefSeq Version	NM_014232.2
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 2 (VAMP2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014232.2

ORF Insert Sequence:

ATGTCTGCTA CCGCTGCCAC GGCCCCCCCT GCTGCCCCGG CTGGGGAGGG TGGTCCCCCT 
GCACCCCCTC CAAACCTCAC CAGTAACAGG AGACTGCAGC AGACCCAGGC CCAGGTGGAT 
GAGGTGGTGG ACATCATGAG GGTGAACGTG GACAAGGTCC TGGAGCGAGA CCAGAAGCTG 
TCGGAGCTGG ACGACCGTGC AGATGCACTC CAGGCGGGGG CCTCCCAGTT TGAAACAAGC 
GCAGCCAAGC TCAAGCGCAA ATACTGGTGG AAAAACCTCA AGATGATGAT CATCTTGGGA 
GTGATTTGCG CCATCATCCT CATCATCATC ATAGTTTACT TCAGCACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP2 ( NM_014232.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055047.2 Homo sapiens vesicle associated membrane protein 2 (VAMP2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu06270
Clone ID Related Accession (Same CDS sequence)	NM_014232.2 , NM_014232.3
Accession Version	NM_014232.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 351bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK225777.1, AK021522.1, AC129492.6 and BU625107.1. On Nov 23, 2018 this sequence version replaced NM_014232.2. Transcript Variant: This variant (1) encodes isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.329161.1, SRR3476690.844899.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000316509.11/ ENSP00000314214.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGCTA CCGCTGCCAC GGCCCCCCCT GCTGCCCCGG CTGGGGAGGG TGGTCCCCCT 
GCACCCCCTC CAAACCTCAC CAGTAACAGG AGACTGCAGC AGACCCAGGC CCAGGTGGAT 
GAGGTGGTGG ACATCATGAG GGTGAACGTG GACAAGGTCC TGGAGCGAGA CCAGAAGCTG 
TCGGAGCTGG ACGACCGTGC AGATGCACTC CAGGCGGGGG CCTCCCAGTT TGAAACAAGC 
GCAGCCAAGC TCAAGCGCAA ATACTGGTGG AAAAACCTCA AGATGATGAT CATCTTGGGA 
GTGATTTGCG CCATCATCCT CATCATCATC ATAGTTTACT TCAGCACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055047.2
CDS	70..420
Translation	MSATAATAPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIVYFST

Target ORF information:

RefSeq Version	NM_014232.3
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 2 (VAMP2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014232.3

ORF Insert Sequence:

ATGTCTGCTA CCGCTGCCAC GGCCCCCCCT GCTGCCCCGG CTGGGGAGGG TGGTCCCCCT 
GCACCCCCTC CAAACCTCAC CAGTAACAGG AGACTGCAGC AGACCCAGGC CCAGGTGGAT 
GAGGTGGTGG ACATCATGAG GGTGAACGTG GACAAGGTCC TGGAGCGAGA CCAGAAGCTG 
TCGGAGCTGG ACGACCGTGC AGATGCACTC CAGGCGGGGG CCTCCCAGTT TGAAACAAGC 
GCAGCCAAGC TCAAGCGCAA ATACTGGTGG AAAAACCTCA AGATGATGAT CATCTTGGGA 
GTGATTTGCG CCATCATCCT CATCATCATC ATAGTTTACT TCAGCACTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VAMP2 ( NM_001330125.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317054.1 Homo sapiens vesicle associated membrane protein 2 (VAMP2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu49924
Clone ID Related Accession (Same CDS sequence)	NM_001330125.1
Accession Version	NM_001330125.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 357bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-15
Organism	Homo sapiens(human)
Product	vesicle-associated membrane protein 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC129492.6 and BU625107.1. On Aug 17, 2016 this sequence version replaced XM_005256775.4. Transcript Variant: This variant (2) differs in the 5' UTR and the 5' coding region compared to variant 1. The encoded isoform (2) has a longer and distinct N-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.306618.1, AA371959.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2144333 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACAGGT CTGCTACCGC TGCCACGGCC CCCCCTGCTG CCCCGGCTGG GGAGGGTGGT 
CCCCCTGCAC CCCCTCCAAA CCTCACCAGT AACAGGAGAC TGCAGCAGAC CCAGGCCCAG 
GTGGATGAGG TGGTGGACAT CATGAGGGTG AACGTGGACA AGGTCCTGGA GCGAGACCAG 
AAGCTGTCGG AGCTGGACGA CCGTGCAGAT GCACTCCAGG CGGGGGCCTC CCAGTTTGAA 
ACAAGCGCAG CCAAGCTCAA GCGCAAATAC TGGTGGAAAA ACCTCAAGAT GATGATCATC 
TTGGGAGTGA TTTGCGCCAT CATCCTCATC ATCATCATAG TTTACTTCAG CACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317054.1
CDS	426..782
Translation	MDRSATAATAPPAAPAGEGGPPAPPPNLTSNRRLQQTQAQVDEVVDIMRVNVDKVLERDQKLSELDDRADALQAGASQFETSAAKLKRKYWWKNLKMMIILGVICAIILIIIIVYFST

Target ORF information:

RefSeq Version	NM_001330125.1
Organism	Homo sapiens(human)
Definition	Homo sapiens vesicle associated membrane protein 2 (VAMP2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001330125.1

ORF Insert Sequence:

ATGGACAGGT CTGCTACCGC TGCCACGGCC CCCCCTGCTG CCCCGGCTGG GGAGGGTGGT 
CCCCCTGCAC CCCCTCCAAA CCTCACCAGT AACAGGAGAC TGCAGCAGAC CCAGGCCCAG 
GTGGATGAGG TGGTGGACAT CATGAGGGTG AACGTGGACA AGGTCCTGGA GCGAGACCAG 
AAGCTGTCGG AGCTGGACGA CCGTGCAGAT GCACTCCAGG CGGGGGCCTC CCAGTTTGAA 
ACAAGCGCAG CCAAGCTCAA GCGCAAATAC TGGTGGAAAA ACCTCAAGAT GATGATCATC 
TTGGGAGTGA TTTGCGCCAT CATCCTCATC ATCATCATAG TTTACTTCAG CACTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

MicroRNA-493-5p promotes apoptosis and suppresses proliferation and invasion in liver cancer cells by targeting VAMP2.
International journal of molecular medicine41(3)1740-1748(2018 Mar)
Wang G,Fang X,Han M,Wang X,Huang Q

?-adrenoceptor activation-increased VAMP-2 and syntaxin-4 in secretory granules are involved in protein secretion of submandibular gland through the PKA/F-actin pathway.
Bioscience reports(2018 Jan)
Ding C,Cong X,Zhang Y,Li SL,Wu LL,Yu GY

?-Synuclein may cross-bridge v-SNARE and acidic phospholipids to facilitate SNARE-dependent vesicle docking.
The Biochemical journal474(12)2039-2049(2017 06)
Lou X,Kim J,Hawk BJ,Shin YK

VAMP2 is implicated in the secretion of antibodies by human plasma cells and can be replaced by other synaptobrevins.
Cellular & molecular immunology(2016 Sep)
G?mez-Jaramillo L,Romero-Garc?a R,Jim?nez-G?mez G,Riegle L,Ramos-Amaya AB,Brieva JA,Kelly-Worden M,Campos-Caro A

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VAMP2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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