MED22 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED22
Entrez Gene ID	6837
Full Name	mediator complex subunit 22
Synonyms	MED24,SRB6,SURF5,surf-5
General protein information	Preferred Names mediator complex subunit 22 Names mediator of RNA polymerase II transcription subunit 22 surfeit locus protein 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 9 Map Location: 9q34.2
Summary	This gene encodes a protein component of the mediator complex, which functions in the regulation of transcription by bridging interactions between gene-specific regulatory factors, RNA polymerase II, and general transcription factors. Alternatively spliced transcript variants encoding different isoforms have been observed. [provided by RefSeq, Jul 2013].
Disorder MIM:	185641

mRNA and Protein(s)

mRNA	Protein	Name
NM_133640.4	NP_598395.1	mediator of RNA polymerase II transcription subunit 22 isoform b
NM_133640.5	NP_598395.1	mediator of RNA polymerase II transcription subunit 22 isoform b
NM_181491.2	NP_852468.1	mediator of RNA polymerase II transcription subunit 22 isoform c

Pathways from BioSystems

Homologies

Homo sapiens (human)	MED22	NP_598395.1
Bos taurus (cattle)	MED22	NP_001029914.1
Mus musculus (house mouse)	Med22	NP_001029080.1
Pan troglodytes (chimpanzee)	MED22	XP_528453.2
Gallus gallus (chicken)	MED22	NP_001039295.1
Xenopus tropicalis (tropical clawed frog)	med22	NP_001120562.1
Canis lupus familiaris (dog)	MED22	XP_005625201.1
Rattus norvegicus (Norway rat)	Med22	NP_001071147.1
Rattus norvegicus (Norway rat)	LOC100911917	XP_003749478.1
Anopheles gambiae (African malaria mosquito)	MED22_ANOGA	XP_313084.3
Danio rerio (zebrafish)	med22	NP_001002144.1
	MED22	XP_001101391.1
Drosophila melanogaster (fruit fly)	MED22	NP_569870.1

Gene Ontology
Bibliography

Related articles in PubMed

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Tsai KL, Tomomori-Sato C, Sato S, Conaway RC, Conaway JW, Asturias FJ
Cell157(6)1430-44(2014 Jun)

Mediator subunits MED1 and MED24 cooperatively contribute to pubertal mammary gland development and growth of breast carcinoma cells.
Hasegawa N, Sumitomo A, Fujita A, Aritome N, Mizuta S, Matsui K, Ishino R, Inoue K, Urahama N, Nose J, Mukohara T, Kamoshida S, Roeder RG, Ito M
Molecular and cellular biology32(8)1483-95(2012 Apr)

The transcriptome of human CD34+ hematopoietic stem-progenitor cells.
Kim YC, Wu Q, Chen J, Xuan Z, Jung YC, Zhang MQ, Rowley JD, Wang SM
Proceedings of the National Academy of Sciences of the United States of America106(20)8278-83(2009 May)

MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription.
Zhang X, Krutchinsky A, Fukuda A, Chen W, Yamamura S, Chait BT, Roeder RG
Molecular cell19(1)89-100(2005 Jul)

A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.
Sato S, Tomomori-Sato C, Parmely TJ, Florens L, Zybailov B, Swanson SK, Banks CA, Jin J, Cai Y, Washburn MP, Conaway JW, Conaway RC
Molecular cell14(5)685-91(2004 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

functional communications between the MED1 subunit and the MED24-containing submodule that mediate estrogen receptor functions and growth of both normal mammary epithelial cells and breast carcinoma cells.
Title: Mediator subunits MED1 and MED24 cooperatively contribute to pubertal mammary gland development and growth of breast carcinoma cells.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

Observational study of gene-disease association. (HuGE Navigator)
Title: Gene-centric association signals for lipids and apolipoproteins identified via the HumanCVD BeadChip.

Hu-Surf5 encodes three different transcripts, Surf-5a, Surf-5b, and Surf-5c, which result from alternative splicing.
Title: The human homologue of the mouse Surf5 gene encodes multiple alternatively spliced transcripts.

This publication also contains human sequence.
Title: Surf5: a gene in the tightly clustered mouse surfeit locus is highly conserved and transcribed divergently from the rpL7A (Surf3) gene.

The following MED22 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED22 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu12174	NM_181491.2 Latest version!	Homo sapiens mediator complex subunit 22 (MED22), transcript variant c, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu06018	NM_133640.5 Latest version!	Homo sapiens mediator complex subunit 22 (MED22), transcript variant b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
View Old Accession Versions >>
OHu06018	NM_133640.4	Homo sapiens mediator complex subunit 22 (MED22), transcript variant b, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED22 ( NM_181491.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_852468.1 Homo sapiens mediator complex subunit 22 (MED22), transcript variant c, mRNA.

Price & Availability↑

CloneID	OHu12174
Clone ID Related Accession (Same CDS sequence)	NM_181491.2
Accession Version	NM_181491.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 423bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-04
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 22 isoform c
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY154869.1, BC024225.2, AL772161.10 and GD160955.1. On Jul 18, 2013 this sequence version replaced NM_181491.1. Transcript Variant: This variant (c) differs in the 3' UTR and its transcription extends past a splice site used in variant b, resulting in a distinct 3' coding region. The encoded isoform (c) is shorter and has a distinct C-terminus, compared to isoform b. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY107566.1, BC040111.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156266, SAMEA2158800 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGC AGAGAGCCCT GCCCCAGAGC AAGGAGACGC TGCTGCAGTC CTACAACAAG 
CGGCTGAAGG ACGACATTAA GTCCATCATG GACAACTTCA CCGAGATCAT CAAGACCGCC 
AAGATTGAGG ACGAGACGCA GGTGTCACGG GCCACTCAGG GTGAACAGGA CAATTACGAG 
ATGCATGTGC GAGCCGCCAA CATCGTCCGA GCCGGCGAGT CCCTGATGAA GCTGGTGTCC 
GACCTCAAGC AGTTCCTGAT CCTCAATGAC TTCCCCTCCG TGAACGAGGC CATTGACCAG 
CGCAACCAGC AGCTGCGCAC ACTGCAGGAG GAGTGCGACC GGAAGCTCAT CACGCTGCGA 
GACGAGATCT CCATTGACCT CTACGAGCTG GAGGAGGAGT ATTACTCGTC CAGGTATAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_852468.1
CDS	274..696
Translation	MAQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISIDLYELEEEYYSSRYK

Target ORF information:

RefSeq Version	NM_181491.2
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 22 (MED22), transcript variant c, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181491.2

ORF Insert Sequence:

ATGGCCCAGC AGAGAGCCCT GCCCCAGAGC AAGGAGACGC TGCTGCAGTC CTACAACAAG 
CGGCTGAAGG ACGACATTAA GTCCATCATG GACAACTTCA CCGAGATCAT CAAGACCGCC 
AAGATTGAGG ACGAGACGCA GGTGTCACGG GCCACTCAGG GTGAACAGGA CAATTACGAG 
ATGCATGTGC GAGCCGCCAA CATCGTCCGA GCCGGCGAGT CCCTGATGAA GCTGGTGTCC 
GACCTCAAGC AGTTCCTGAT CCTCAATGAC TTCCCCTCCG TGAACGAGGC CATTGACCAG 
CGCAACCAGC AGCTGCGCAC ACTGCAGGAG GAGTGCGACC GGAAGCTCAT CACGCTGCGA 
GACGAGATCT CCATTGACCT CTACGAGCTG GAGGAGGAGT ATTACTCGTC CAGGTATAAA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED22 ( NM_133640.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_598395.1 Homo sapiens mediator complex subunit 22 (MED22), transcript variant b, mRNA.

Price & Availability↑

CloneID	OHu06018
Clone ID Related Accession (Same CDS sequence)	NM_133640.4 , NM_133640.5
Accession Version	NM_133640.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-25
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 22 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY154869.1, AJ224359.1 and GD160955.1. On Jul 18, 2013 this sequence version replaced NM_133640.3. Transcript Variant: This variant (b) represents the shorter transcript and encodes the longer isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ224359.1, BI488406.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGC AGAGAGCCCT GCCCCAGAGC AAGGAGACGC TGCTGCAGTC CTACAACAAG 
CGGCTGAAGG ACGACATTAA GTCCATCATG GACAACTTCA CCGAGATCAT CAAGACCGCC 
AAGATTGAGG ACGAGACGCA GGTGTCACGG GCCACTCAGG GTGAACAGGA CAATTACGAG 
ATGCATGTGC GAGCCGCCAA CATCGTCCGA GCCGGCGAGT CCCTGATGAA GCTGGTGTCC 
GACCTCAAGC AGTTCCTGAT CCTCAATGAC TTCCCCTCCG TGAACGAGGC CATTGACCAG 
CGCAACCAGC AGCTGCGCAC ACTGCAGGAG GAGTGCGACC GGAAGCTCAT CACGCTGCGA 
GACGAGATCT CCATTGACCT CTACGAGCTG GAGGAGGAGT ATTACTCGTC CAGCTCAAGC 
CTTTGCGAAG CTAATGACCT GCCTCTGTGC GAAGCTTACG GGAGGCTGGA CCTCGACACA 
GACTCTGCTG ATGGCCTCTC GGCCCCTCTG CTGGCGTCCC CGGAGCCCAG TGCTGGCCCC 
CTACAGGTGG CAGCCCCTGC CCACTCCCAT GCTGGTGGCC CTGGCCCCAC TGAGCACGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598395.1
CDS	274..876
Translation	MAQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISIDLYELEEEYYSSSSSLCEANDLPLCEAYGRLDLDTDSADGLSAPLLASPEPSAGPLQVAAPAHSHAGGPGPTEHA

Target ORF information:

RefSeq Version	NM_133640.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 22 (MED22), transcript variant b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133640.4

ORF Insert Sequence:

ATGGCCCAGC AGAGAGCCCT GCCCCAGAGC AAGGAGACGC TGCTGCAGTC CTACAACAAG 
CGGCTGAAGG ACGACATTAA GTCCATCATG GACAACTTCA CCGAGATCAT CAAGACCGCC 
AAGATTGAGG ACGAGACGCA GGTGTCACGG GCCACTCAGG GTGAACAGGA CAATTACGAG 
ATGCATGTGC GAGCCGCCAA CATCGTCCGA GCCGGCGAGT CCCTGATGAA GCTGGTGTCC 
GACCTCAAGC AGTTCCTGAT CCTCAATGAC TTCCCCTCCG TGAACGAGGC CATTGACCAG 
CGCAACCAGC AGCTGCGCAC ACTGCAGGAG GAGTGCGACC GGAAGCTCAT CACGCTGCGA 
GACGAGATCT CCATTGACCT CTACGAGCTG GAGGAGGAGT ATTACTCGTC CAGCTCAAGC 
CTTTGCGAAG CTAATGACCT GCCTCTGTGC GAAGCTTACG GGAGGCTGGA CCTCGACACA 
GACTCTGCTG ATGGCCTCTC GGCCCCTCTG CTGGCGTCCC CGGAGCCCAG TGCTGGCCCC 
CTACAGGTGG CAGCCCCTGC CCACTCCCAT GCTGGTGGCC CTGGCCCCAC TGAGCACGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED22 ( NM_133640.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_598395.1 Homo sapiens mediator complex subunit 22 (MED22), transcript variant b, mRNA.

Price & Availability↑

CloneID	OHu06018
Clone ID Related Accession (Same CDS sequence)	NM_133640.4 , NM_133640.5
Accession Version	NM_133640.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	mediator of RNA polymerase II transcription subunit 22 isoform b
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from HY154869.1, AJ224359.1, GD160955.1 and AL772161.10. On May 17, 2019 this sequence version replaced NM_133640.4. Transcript Variant: This variant (b) represents the shorter transcript and encodes the longer isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7410570.438444.1, SRR3476690.583551.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000343730.10/ ENSP00000342343.5 RefSeq Select criteria :: based on conservation, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCCAGC AGAGAGCCCT GCCCCAGAGC AAGGAGACGC TGCTGCAGTC CTACAACAAG 
CGGCTGAAGG ACGACATTAA GTCCATCATG GACAACTTCA CCGAGATCAT CAAGACCGCC 
AAGATTGAGG ACGAGACGCA GGTGTCACGG GCCACTCAGG GTGAACAGGA CAATTACGAG 
ATGCATGTGC GAGCCGCCAA CATCGTCCGA GCCGGCGAGT CCCTGATGAA GCTGGTGTCC 
GACCTCAAGC AGTTCCTGAT CCTCAATGAC TTCCCCTCCG TGAACGAGGC CATTGACCAG 
CGCAACCAGC AGCTGCGCAC ACTGCAGGAG GAGTGCGACC GGAAGCTCAT CACGCTGCGA 
GACGAGATCT CCATTGACCT CTACGAGCTG GAGGAGGAGT ATTACTCGTC CAGCTCAAGC 
CTTTGCGAAG CTAATGACCT GCCTCTGTGC GAAGCTTACG GGAGGCTGGA CCTCGACACA 
GACTCTGCTG ATGGCCTCTC GGCCCCTCTG CTGGCGTCCC CGGAGCCCAG TGCTGGCCCC 
CTACAGGTGG CAGCCCCTGC CCACTCCCAT GCTGGTGGCC CTGGCCCCAC TGAGCACGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_598395.1
CDS	249..851
Translation	MAQQRALPQSKETLLQSYNKRLKDDIKSIMDNFTEIIKTAKIEDETQVSRATQGEQDNYEMHVRAANIVRAGESLMKLVSDLKQFLILNDFPSVNEAIDQRNQQLRTLQEECDRKLITLRDEISIDLYELEEEYYSSSSSLCEANDLPLCEAYGRLDLDTDSADGLSAPLLASPEPSAGPLQVAAPAHSHAGGPGPTEHA

Target ORF information:

RefSeq Version	NM_133640.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mediator complex subunit 22 (MED22), transcript variant b, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_133640.5

ORF Insert Sequence:

ATGGCCCAGC AGAGAGCCCT GCCCCAGAGC AAGGAGACGC TGCTGCAGTC CTACAACAAG 
CGGCTGAAGG ACGACATTAA GTCCATCATG GACAACTTCA CCGAGATCAT CAAGACCGCC 
AAGATTGAGG ACGAGACGCA GGTGTCACGG GCCACTCAGG GTGAACAGGA CAATTACGAG 
ATGCATGTGC GAGCCGCCAA CATCGTCCGA GCCGGCGAGT CCCTGATGAA GCTGGTGTCC 
GACCTCAAGC AGTTCCTGAT CCTCAATGAC TTCCCCTCCG TGAACGAGGC CATTGACCAG 
CGCAACCAGC AGCTGCGCAC ACTGCAGGAG GAGTGCGACC GGAAGCTCAT CACGCTGCGA 
GACGAGATCT CCATTGACCT CTACGAGCTG GAGGAGGAGT ATTACTCGTC CAGCTCAAGC 
CTTTGCGAAG CTAATGACCT GCCTCTGTGC GAAGCTTACG GGAGGCTGGA CCTCGACACA 
GACTCTGCTG ATGGCCTCTC GGCCCCTCTG CTGGCGTCCC CGGAGCCCAG TGCTGGCCCC 
CTACAGGTGG CAGCCCCTGC CCACTCCCAT GCTGGTGGCC CTGGCCCCAC TGAGCACGCC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Subunit architecture and functional modular rearrangements of the transcriptional mediator complex.
Cell157(6)1430-44(2014 Jun)
Tsai KL,Tomomori-Sato C,Sato S,Conaway RC,Conaway JW,Asturias FJ

Mediator subunits MED1 and MED24 cooperatively contribute to pubertal mammary gland development and growth of breast carcinoma cells.
Molecular and cellular biology32(8)1483-95(2012 Apr)
Hasegawa N,Sumitomo A,Fujita A,Aritome N,Mizuta S,Matsui K,Ishino R,Inoue K,Urahama N,Nose J,Mukohara T,Kamoshida S,Roeder RG,Ito M

The transcriptome of human CD34+ hematopoietic stem-progenitor cells.
Proceedings of the National Academy of Sciences of the United States of America106(20)8278-83(2009 May)
Kim YC,Wu Q,Chen J,Xuan Z,Jung YC,Zhang MQ,Rowley JD,Wang SM

MED1/TRAP220 exists predominantly in a TRAP/ Mediator subpopulation enriched in RNA polymerase II and is required for ER-mediated transcription.
Molecular cell19(1)89-100(2005 Jul)
Zhang X,Krutchinsky A,Fukuda A,Chen W,Yamamura S,Chait BT,Roeder RG

A set of consensus mammalian mediator subunits identified by multidimensional protein identification technology.
Molecular cell14(5)685-91(2004 Jun)
Sato S,Tomomori-Sato C,Parmely TJ,Florens L,Zybailov B,Swanson SK,Banks CA,Jin J,Cai Y,Washburn MP,Conaway JW,Conaway RC

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MED22 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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