SPTBN2 cDNA ORF clone, Homo sapiens(human)

The following SPTBN2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu60720 XM_011545216.3
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu60721 XM_011545217.2
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu45471 NM_006946.4
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 29-31 $1301.30
$1859.00
OHu27129 NM_006946.3
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu45470 XM_006718669.3
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu45471 XM_006718671.4
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu45471 XM_017018178.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu45471 XM_017018175.2
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu45471 XM_017018174.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu45471 XM_005274193.3
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu45471 XM_017018177.2
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu45471 XM_017018176.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu45471 XM_005274192.4
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
View Old Accession Versions >>
OHu45471 XM_017018175.1 Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu45471 XM_017018177.1 Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu27129 NM_006946.2 Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $923.30
$1319.00
OHu60720 XM_011545216.2 Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu45471 XM_006718671.3 Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu60720
    Clone ID Related Accession (Same CDS sequence) XM_011545216.3 , XM_011545216.2
    Accession Version XM_011545216.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011545216.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGGGA ACGGAGTGAG CAGGGTGCAC GGGAACGGCC TGCATGAGGC CAGCGAGTTT 
    TACTATGAGG CTGTGGAAGG GGCCCACAAC CCCGGGGGCC TCCTGCTTTC ACCAGCTGCT
    TTCATCAACC CTGCTCAGTA TGCCAGCGTG CTGGAAGGAC GCTTCAAACA GCTGCAAGAT
    GAACGAGAAG CTGTGCAGAA GAAAACCTTC ACCAAGTGGG TAAACTCGCA CCTGGCCCGG
    GTCACGTGCC GGGTGGGGGA CCTGTACAGC GACCTCCGGG ACGGACGCAA CCTGCTGAGG
    CTCCTCGAGG TGCTCTCGGG AGAGATACTG CCAAAGCCTA CAAAGGGCCG CATGCGGATC
    CACTGCCTGG AGAACGTGGA CAAGGCACTG CAGTTCCTCA AGGAGCAGAA AGTGCACTTG
    GAAAACATGG GCTCCCATGA CATTGTGGAC GGAAACCACC GACTGACCCT TGGGCTGGTC
    TGGACCATCA TCCTTCGATT CCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG
    GAGAAGAAGT CAGCCAAGGA TGCCCTGCTT CTGTGGTGCC AGATGAAGAC TGCAGGTTAT
    CCCAACGTCA ATGTACACAA CTTCACCACC AGCTGGAGAG ATGGACTAGC TTTCAACGCC
    ATCGTGCATA AACACCGGCC AGACCTGCTG GATTTTGAGT CTCTGAAGAA GTGTAATGCA
    CACTATAATC TGCAGAATGC ATTCAATCTG GCTGAAAAGG AACTGGGACT TACCAAGCTG
    CTGGATCCCG AAGACGTGAA TGTGGACCAG CCAGATGAGA AGTCAATCAT TACCTATGTG
    GCTACTTACT ACCATTACTT CTCCAAGATG AAGGCCCTGG CCGTGGAAGG CAAGAGAATT
    GGCAAGGTGC TGGACCATGC CATGGAGGCA GAGCGCCTGG TGGAGAAATA CGAGTCCCTG
    GCCTCGGAGC TGCTGCAGTG GATCGAGCAA ACGATCGTGA CCCTCAATGA CCGGCAGTTG
    GCCAACTCCC TTAGCGGGGT CCAGAACCAG CTGCAGTCCT TCAACTCCTA CCGCACCGTG
    GAGAAGCCGC CCAAGTTTAC CGAGAAAGGG AACTTGGAAG TGCTGCTCTT CACCATCCAG
    AGCAAGCTTC GGGCCAACAA CCAGAAGGTC TACACGCCCC GCGAGGGCCG GCTCATCTCG
    GACATCAACA AGGCTTGGGA GCGGCTGGAG AAGGCGGAGC ACGAGCGTGA GCTGGCCCTG
    CGCACCGAGC TCATCCGCCA GGAGAAGCTG GAGCAGCTGG CCGCCCGCTT CGACCGCAAG
    GCTGCCATGC GGGAGACCTG GCTCAGCGAG AACCAGCGCC TCGTGTCCCA GGACAACTTT
    GGGCTGGAGC TGGCAGCTGT CGAGGCAGCA GTACGGAAGC ACGAAGCCAT TGAGACGGAC
    ATCGTGGCCT ACAGCGGCCG GGTGCAGGCA GTGGACGCCG TGGCTGCAGA GCTGGCCGCC
    GAGCGCTACC ACGACATCAA GCGCATCGCC GCTCGGCAGC ACAACGTGGC ACGGCTCTGG
    GACTTCTTGC GGCAGATGGT GGCCGCCCGG CGGGAGCGGC TCCTCCTCAA CCTGGAGCTG
    CAGAAGGTGT TCCAGGACCT GCTCTACCTC ATGGACTGGA TGGAAGAGAT GAAGGGCCGG
    CTGCAGTCTC AGGACCTGGG CAGGCACCTA GCAGGAGTGG AGGACCTGCT GCAGCTGCAC
    GAGCTGGTGG AGGCAGACAT CGCCGTGCAG GCCGAGAGGG TGCGGGCCGT CAGCGCCTCT
    GCCCTGCGCT TCTGCAACCC AGGGAAAGAG TATAGACCTT GCGACCCGCA GCTGGTGTCG
    GAGCGGGTGG CCAAGCTAGA GCAGAGCTAT GAGGCACTGT GCGAGTTGGC AGCGGCGCGG
    CGGGCCCGGC TGGAGGAATC ACGGCGGCTC TGGCGTTTCC TCTGGGAGGT GGGTGAAGCT
    GAGGCCTGGG TGCGGGAGCA GCAGCACCTC CTGGCCTCAG CCGACACGGG CCGAGACCTG
    ACCGGTGCCC TCCGCCTGCT CAACAAGCAC ACAGCCCTGC GGGGCGAGAT GAGCGGCCGG
    CTGGGGCCCC TGAAGCTCAC CCTGGAGCAG GGCCAGCAGT TGGTGGCCGA GGGTCACCCT
    GGGGCAAGCC AGGCCTCTGC CCGTGCAGCT GAACTCCAAG CCCAGTGGGA GCGGCTAGAG
    GCCCTGGCCG AGGAGCGTGC CCAGCGGCTG GCCCAAGCCG CCAGCCTCTA CCAGTTCCAG
    GCCGATGCAA ACGACATGGA GGCCTGGTTG GTTGACGCAC TGCGCCTGGT GTCCAGCCCC
    GAGCTGGGGC ACGACGAGTT CTCCACGCAG GCTCTAGCCA GGCAGCATCG GGCCCTGGAG
    GAGGAGATTC GAAGCCACCG GCCAACCCTG GACGCCTTGA GGGAACAGGC AGCAGCCCTG
    CCCCCCACAC TGAGCCGCAC GCCCGAGGTG CAGAGCCGGG TGCCCACCCT GGAGCGGCAC
    TACGAGGAGC TGCAGGCCCG GGCAGGCGAG CGAGCGCGGG CCTTGGAGGC AGCCCTGGCG
    CTCTACACCA TGCTCAGCGA GGCCGGGGCC TGTGGACTCT GGGTGGAGGA GAAGGAGCAG
    TGGCTCAACG GGCTGGCCCT GCCTGAACGC CTGGAGGACC TGGAGGTCGT GCAGCAGAGG
    TTCGAGACCC TGGAGCCTGA AATGAACACC CTTGCAGCAC AAATCACCGC GGTGAATGAC
    ATTGCCGAGC AGTTACTGAA GGCCAACCCC CCAGGCAAAG ACCGCATTGT CAACACCCAG
    GAGCAGCTCA ACCACAGGTG GCAGCAGTTT CGGCGTCTGG CAGACGGCAA GAAGGCAGCT
    CTCACCTCAG CCCTGAGCAT CCAGAACTAC CACTTAGAGT GCACGGAGAC CCAGGCCTGG
    ATGAGAGAGA AGACCAAAGT CATCGAGTCC ACCCAGGGCC TAGGCAACGA TCTGGCTGGG
    GTGCTGGCCC TGCAGCGCAA GCTGGCCGGC ACGGAGCGGG ACCTGGAGGC CATCGCCGCC
    CGGGTGGGCG AACTGACTCG AGAGGCAAAT GCCCTGGCTG CCGGCCATCC CGCTCAGGCA
    GTGGCCATCA ACGCCCGGCT GAGAGAGGTG CAGACCGGCT GGGAGGACCT CAGGGCCACC
    ATGCGGCGTC GAGAAGAGTC GCTGGGGGAG GCGCGGCGGC TGCAGGACTT CTTGCGCAGC
    TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACTCAGACTG CTGTGGCCTC TGAAGAAGGG
    CCGGCCACCC TGCCTGAGGC AGAGGCCCTC CTGGCCCAAC ATGCAGCCCT GCGGGGAGAG
    GTGGAGCGGG CCCAGAGCGA GTATAGCCGG CTGCGAGCCC TGGGCGAGGA GGTGACCCGG
    GACCAGGCTG ACCCCCAGTG CCTCTTCCTA CGACAGCGAC TGGAGGCCCT GGGAACTGGC
    TGGGAGGAGC TGGGCCGAAT GTGGGAGAGC CGGCAAGGTC GCCTGGCCCA GGCCCACGGC
    TTCCAGGGAT TCCTGCGGGA TGCTCGTCAG GCTGAGGGCG TGCTCAGCAG CCAGGAATAT
    GTTCTGTCTC ACACGGAGAT GCCAGGGACA CTCCAGGCTG CTGATGCTGC CATTAAAAAA
    CTGGAGGACT TCATGAGCAC CATGGACGCC AATGGGGAAC GGATCCACGG GCTCCTGGAG
    GCTGGCCGCC AGCTGGTATC TGAAGGCAAC ATCCACGCCG ACAAGATTCG GGAAAAGGCA
    GACTCCATTG AGAGGAGGCA CAAGAAGAAT CAAGACGCAG CGCAGCAATT TCTGGGCCGT
    CTTCGGGACA ACCGGGAGCA GCAGCATTTC CTGCAAGATT GTCACGAGCT GAAGCTCTGG
    ATCGACGAGA AGATGCTGAC AGCCCAGGAC GTGTCCTATG ACGAGGCCCG CAACCTGCAT
    ACTAAGTGGC AGAAGCACCA GGCATTCATG GCCGAGCTGG CTGCCAACAA AGACTGGCTG
    GACAAGGTGG ACAAGGCAGT GGGCCAGATT ACCCCGTGGG CTGTATGTAG TCTGCCAATC
    CTTATTTTCG AGTCTTGCCG TCCCTGGTTC TTGGTCTCCC ACCCAGCTGT TCCTCAAGAG
    TCAAGGACAG ATAAAGCTTT ATATCTGCTC AGCAAGTATC CCTACAGAAC ATGGTACGTG
    CTCAACAGTC TGCGAGGCAC TGCAGGGTCT ACGAAAAGAC ACTTTCCTTT TCTCAAGGGC
    ATGATGGTTG GGACAAAAGG AGACCAGTGT GCTTTCAAGT GTTTCCATTC GTTCAGCAAA
    TATTTACTTG GTGCTACTAT ATCCAAAGCA TTGTATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543518.1
    CDS2411..6769
    Translation

    Target ORF information:

    RefSeq Version XM_011545216.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545216.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGGGA ACGGAGTGAG CAGGGTGCAC GGGAACGGCC TGCATGAGGC CAGCGAGTTT 
    TACTATGAGG CTGTGGAAGG GGCCCACAAC CCCGGGGGCC TCCTGCTTTC ACCAGCTGCT
    TTCATCAACC CTGCTCAGTA TGCCAGCGTG CTGGAAGGAC GCTTCAAACA GCTGCAAGAT
    GAACGAGAAG CTGTGCAGAA GAAAACCTTC ACCAAGTGGG TAAACTCGCA CCTGGCCCGG
    GTCACGTGCC GGGTGGGGGA CCTGTACAGC GACCTCCGGG ACGGACGCAA CCTGCTGAGG
    CTCCTCGAGG TGCTCTCGGG AGAGATACTG CCAAAGCCTA CAAAGGGCCG CATGCGGATC
    CACTGCCTGG AGAACGTGGA CAAGGCACTG CAGTTCCTCA AGGAGCAGAA AGTGCACTTG
    GAAAACATGG GCTCCCATGA CATTGTGGAC GGAAACCACC GACTGACCCT TGGGCTGGTC
    TGGACCATCA TCCTTCGATT CCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG
    GAGAAGAAGT CAGCCAAGGA TGCCCTGCTT CTGTGGTGCC AGATGAAGAC TGCAGGTTAT
    CCCAACGTCA ATGTACACAA CTTCACCACC AGCTGGAGAG ATGGACTAGC TTTCAACGCC
    ATCGTGCATA AACACCGGCC AGACCTGCTG GATTTTGAGT CTCTGAAGAA GTGTAATGCA
    CACTATAATC TGCAGAATGC ATTCAATCTG GCTGAAAAGG AACTGGGACT TACCAAGCTG
    CTGGATCCCG AAGACGTGAA TGTGGACCAG CCAGATGAGA AGTCAATCAT TACCTATGTG
    GCTACTTACT ACCATTACTT CTCCAAGATG AAGGCCCTGG CCGTGGAAGG CAAGAGAATT
    GGCAAGGTGC TGGACCATGC CATGGAGGCA GAGCGCCTGG TGGAGAAATA CGAGTCCCTG
    GCCTCGGAGC TGCTGCAGTG GATCGAGCAA ACGATCGTGA CCCTCAATGA CCGGCAGTTG
    GCCAACTCCC TTAGCGGGGT CCAGAACCAG CTGCAGTCCT TCAACTCCTA CCGCACCGTG
    GAGAAGCCGC CCAAGTTTAC CGAGAAAGGG AACTTGGAAG TGCTGCTCTT CACCATCCAG
    AGCAAGCTTC GGGCCAACAA CCAGAAGGTC TACACGCCCC GCGAGGGCCG GCTCATCTCG
    GACATCAACA AGGCTTGGGA GCGGCTGGAG AAGGCGGAGC ACGAGCGTGA GCTGGCCCTG
    CGCACCGAGC TCATCCGCCA GGAGAAGCTG GAGCAGCTGG CCGCCCGCTT CGACCGCAAG
    GCTGCCATGC GGGAGACCTG GCTCAGCGAG AACCAGCGCC TCGTGTCCCA GGACAACTTT
    GGGCTGGAGC TGGCAGCTGT CGAGGCAGCA GTACGGAAGC ACGAAGCCAT TGAGACGGAC
    ATCGTGGCCT ACAGCGGCCG GGTGCAGGCA GTGGACGCCG TGGCTGCAGA GCTGGCCGCC
    GAGCGCTACC ACGACATCAA GCGCATCGCC GCTCGGCAGC ACAACGTGGC ACGGCTCTGG
    GACTTCTTGC GGCAGATGGT GGCCGCCCGG CGGGAGCGGC TCCTCCTCAA CCTGGAGCTG
    CAGAAGGTGT TCCAGGACCT GCTCTACCTC ATGGACTGGA TGGAAGAGAT GAAGGGCCGG
    CTGCAGTCTC AGGACCTGGG CAGGCACCTA GCAGGAGTGG AGGACCTGCT GCAGCTGCAC
    GAGCTGGTGG AGGCAGACAT CGCCGTGCAG GCCGAGAGGG TGCGGGCCGT CAGCGCCTCT
    GCCCTGCGCT TCTGCAACCC AGGGAAAGAG TATAGACCTT GCGACCCGCA GCTGGTGTCG
    GAGCGGGTGG CCAAGCTAGA GCAGAGCTAT GAGGCACTGT GCGAGTTGGC AGCGGCGCGG
    CGGGCCCGGC TGGAGGAATC ACGGCGGCTC TGGCGTTTCC TCTGGGAGGT GGGTGAAGCT
    GAGGCCTGGG TGCGGGAGCA GCAGCACCTC CTGGCCTCAG CCGACACGGG CCGAGACCTG
    ACCGGTGCCC TCCGCCTGCT CAACAAGCAC ACAGCCCTGC GGGGCGAGAT GAGCGGCCGG
    CTGGGGCCCC TGAAGCTCAC CCTGGAGCAG GGCCAGCAGT TGGTGGCCGA GGGTCACCCT
    GGGGCAAGCC AGGCCTCTGC CCGTGCAGCT GAACTCCAAG CCCAGTGGGA GCGGCTAGAG
    GCCCTGGCCG AGGAGCGTGC CCAGCGGCTG GCCCAAGCCG CCAGCCTCTA CCAGTTCCAG
    GCCGATGCAA ACGACATGGA GGCCTGGTTG GTTGACGCAC TGCGCCTGGT GTCCAGCCCC
    GAGCTGGGGC ACGACGAGTT CTCCACGCAG GCTCTAGCCA GGCAGCATCG GGCCCTGGAG
    GAGGAGATTC GAAGCCACCG GCCAACCCTG GACGCCTTGA GGGAACAGGC AGCAGCCCTG
    CCCCCCACAC TGAGCCGCAC GCCCGAGGTG CAGAGCCGGG TGCCCACCCT GGAGCGGCAC
    TACGAGGAGC TGCAGGCCCG GGCAGGCGAG CGAGCGCGGG CCTTGGAGGC AGCCCTGGCG
    CTCTACACCA TGCTCAGCGA GGCCGGGGCC TGTGGACTCT GGGTGGAGGA GAAGGAGCAG
    TGGCTCAACG GGCTGGCCCT GCCTGAACGC CTGGAGGACC TGGAGGTCGT GCAGCAGAGG
    TTCGAGACCC TGGAGCCTGA AATGAACACC CTTGCAGCAC AAATCACCGC GGTGAATGAC
    ATTGCCGAGC AGTTACTGAA GGCCAACCCC CCAGGCAAAG ACCGCATTGT CAACACCCAG
    GAGCAGCTCA ACCACAGGTG GCAGCAGTTT CGGCGTCTGG CAGACGGCAA GAAGGCAGCT
    CTCACCTCAG CCCTGAGCAT CCAGAACTAC CACTTAGAGT GCACGGAGAC CCAGGCCTGG
    ATGAGAGAGA AGACCAAAGT CATCGAGTCC ACCCAGGGCC TAGGCAACGA TCTGGCTGGG
    GTGCTGGCCC TGCAGCGCAA GCTGGCCGGC ACGGAGCGGG ACCTGGAGGC CATCGCCGCC
    CGGGTGGGCG AACTGACTCG AGAGGCAAAT GCCCTGGCTG CCGGCCATCC CGCTCAGGCA
    GTGGCCATCA ACGCCCGGCT GAGAGAGGTG CAGACCGGCT GGGAGGACCT CAGGGCCACC
    ATGCGGCGTC GAGAAGAGTC GCTGGGGGAG GCGCGGCGGC TGCAGGACTT CTTGCGCAGC
    TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACTCAGACTG CTGTGGCCTC TGAAGAAGGG
    CCGGCCACCC TGCCTGAGGC AGAGGCCCTC CTGGCCCAAC ATGCAGCCCT GCGGGGAGAG
    GTGGAGCGGG CCCAGAGCGA GTATAGCCGG CTGCGAGCCC TGGGCGAGGA GGTGACCCGG
    GACCAGGCTG ACCCCCAGTG CCTCTTCCTA CGACAGCGAC TGGAGGCCCT GGGAACTGGC
    TGGGAGGAGC TGGGCCGAAT GTGGGAGAGC CGGCAAGGTC GCCTGGCCCA GGCCCACGGC
    TTCCAGGGAT TCCTGCGGGA TGCTCGTCAG GCTGAGGGCG TGCTCAGCAG CCAGGAATAT
    GTTCTGTCTC ACACGGAGAT GCCAGGGACA CTCCAGGCTG CTGATGCTGC CATTAAAAAA
    CTGGAGGACT TCATGAGCAC CATGGACGCC AATGGGGAAC GGATCCACGG GCTCCTGGAG
    GCTGGCCGCC AGCTGGTATC TGAAGGCAAC ATCCACGCCG ACAAGATTCG GGAAAAGGCA
    GACTCCATTG AGAGGAGGCA CAAGAAGAAT CAAGACGCAG CGCAGCAATT TCTGGGCCGT
    CTTCGGGACA ACCGGGAGCA GCAGCATTTC CTGCAAGATT GTCACGAGCT GAAGCTCTGG
    ATCGACGAGA AGATGCTGAC AGCCCAGGAC GTGTCCTATG ACGAGGCCCG CAACCTGCAT
    ACTAAGTGGC AGAAGCACCA GGCATTCATG GCCGAGCTGG CTGCCAACAA AGACTGGCTG
    GACAAGGTGG ACAAGGCAGT GGGCCAGATT ACCCCGTGGG CTGTATGTAG TCTGCCAATC
    CTTATTTTCG AGTCTTGCCG TCCCTGGTTC TTGGTCTCCC ACCCAGCTGT TCCTCAAGAG
    TCAAGGACAG ATAAAGCTTT ATATCTGCTC AGCAAGTATC CCTACAGAAC ATGGTACGTG
    CTCAACAGTC TGCGAGGCAC TGCAGGGTCT ACGAAAAGAC ACTTTCCTTT TCTCAAGGGC
    ATGATGGTTG GGACAAAAGG AGACCAGTGT GCTTTCAAGT GTTTCCATTC GTTCAGCAAA
    TATTTACTTG GTGCTACTAT ATCCAAAGCA TTGTATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60720
    Clone ID Related Accession (Same CDS sequence) XM_011545216.3 , XM_011545216.2
    Accession Version XM_011545216.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4359bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545216.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGGGA ACGGAGTGAG CAGGGTGCAC GGGAACGGCC TGCATGAGGC CAGCGAGTTT 
    TACTATGAGG CTGTGGAAGG GGCCCACAAC CCCGGGGGCC TCCTGCTTTC ACCAGCTGCT
    TTCATCAACC CTGCTCAGTA TGCCAGCGTG CTGGAAGGAC GCTTCAAACA GCTGCAAGAT
    GAACGAGAAG CTGTGCAGAA GAAAACCTTC ACCAAGTGGG TAAACTCGCA CCTGGCCCGG
    GTCACGTGCC GGGTGGGGGA CCTGTACAGC GACCTCCGGG ACGGACGCAA CCTGCTGAGG
    CTCCTCGAGG TGCTCTCGGG AGAGATACTG CCAAAGCCTA CAAAGGGCCG CATGCGGATC
    CACTGCCTGG AGAACGTGGA CAAGGCACTG CAGTTCCTCA AGGAGCAGAA AGTGCACTTG
    GAAAACATGG GCTCCCATGA CATTGTGGAC GGAAACCACC GACTGACCCT TGGGCTGGTC
    TGGACCATCA TCCTTCGATT CCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG
    GAGAAGAAGT CAGCCAAGGA TGCCCTGCTT CTGTGGTGCC AGATGAAGAC TGCAGGTTAT
    CCCAACGTCA ATGTACACAA CTTCACCACC AGCTGGAGAG ATGGACTAGC TTTCAACGCC
    ATCGTGCATA AACACCGGCC AGACCTGCTG GATTTTGAGT CTCTGAAGAA GTGTAATGCA
    CACTATAATC TGCAGAATGC ATTCAATCTG GCTGAAAAGG AACTGGGACT TACCAAGCTG
    CTGGATCCCG AAGACGTGAA TGTGGACCAG CCAGATGAGA AGTCAATCAT TACCTATGTG
    GCTACTTACT ACCATTACTT CTCCAAGATG AAGGCCCTGG CCGTGGAAGG CAAGAGAATT
    GGCAAGGTGC TGGACCATGC CATGGAGGCA GAGCGCCTGG TGGAGAAATA CGAGTCCCTG
    GCCTCGGAGC TGCTGCAGTG GATCGAGCAA ACGATCGTGA CCCTCAATGA CCGGCAGTTG
    GCCAACTCCC TTAGCGGGGT CCAGAACCAG CTGCAGTCCT TCAACTCCTA CCGCACCGTG
    GAGAAGCCGC CCAAGTTTAC CGAGAAAGGG AACTTGGAAG TGCTGCTCTT CACCATCCAG
    AGCAAGCTTC GGGCCAACAA CCAGAAGGTC TACACGCCCC GCGAGGGCCG GCTCATCTCG
    GACATCAACA AGGCTTGGGA GCGGCTGGAG AAGGCGGAGC ACGAGCGTGA GCTGGCCCTG
    CGCACCGAGC TCATCCGCCA GGAGAAGCTG GAGCAGCTGG CCGCCCGCTT CGACCGCAAG
    GCTGCCATGC GGGAGACCTG GCTCAGCGAG AACCAGCGCC TCGTGTCCCA GGACAACTTT
    GGGCTGGAGC TGGCAGCTGT CGAGGCAGCA GTACGGAAGC ACGAAGCCAT TGAGACGGAC
    ATCGTGGCCT ACAGCGGCCG GGTGCAGGCA GTGGACGCCG TGGCTGCAGA GCTGGCCGCC
    GAGCGCTACC ACGACATCAA GCGCATCGCC GCTCGGCAGC ACAACGTGGC ACGGCTCTGG
    GACTTCTTGC GGCAGATGGT GGCCGCCCGG CGGGAGCGGC TCCTCCTCAA CCTGGAGCTG
    CAGAAGGTGT TCCAGGACCT GCTCTACCTC ATGGACTGGA TGGAAGAGAT GAAGGGCCGG
    CTGCAGTCTC AGGACCTGGG CAGGCACCTA GCAGGAGTGG AGGACCTGCT GCAGCTGCAC
    GAGCTGGTGG AGGCAGACAT CGCCGTGCAG GCCGAGAGGG TGCGGGCCGT CAGCGCCTCT
    GCCCTGCGCT TCTGCAACCC AGGGAAAGAG TATAGACCTT GCGACCCGCA GCTGGTGTCG
    GAGCGGGTGG CCAAGCTAGA GCAGAGCTAT GAGGCACTGT GCGAGTTGGC AGCGGCGCGG
    CGGGCCCGGC TGGAGGAATC ACGGCGGCTC TGGCGTTTCC TCTGGGAGGT GGGTGAAGCT
    GAGGCCTGGG TGCGGGAGCA GCAGCACCTC CTGGCCTCAG CCGACACGGG CCGAGACCTG
    ACCGGTGCCC TCCGCCTGCT CAACAAGCAC ACAGCCCTGC GGGGCGAGAT GAGCGGCCGG
    CTGGGGCCCC TGAAGCTCAC CCTGGAGCAG GGCCAGCAGT TGGTGGCCGA GGGTCACCCT
    GGGGCAAGCC AGGCCTCTGC CCGTGCAGCT GAACTCCAAG CCCAGTGGGA GCGGCTAGAG
    GCCCTGGCCG AGGAGCGTGC CCAGCGGCTG GCCCAAGCCG CCAGCCTCTA CCAGTTCCAG
    GCCGATGCAA ACGACATGGA GGCCTGGTTG GTTGACGCAC TGCGCCTGGT GTCCAGCCCC
    GAGCTGGGGC ACGACGAGTT CTCCACGCAG GCTCTAGCCA GGCAGCATCG GGCCCTGGAG
    GAGGAGATTC GAAGCCACCG GCCAACCCTG GACGCCTTGA GGGAACAGGC AGCAGCCCTG
    CCCCCCACAC TGAGCCGCAC GCCCGAGGTG CAGAGCCGGG TGCCCACCCT GGAGCGGCAC
    TACGAGGAGC TGCAGGCCCG GGCAGGCGAG CGAGCGCGGG CCTTGGAGGC AGCCCTGGCG
    CTCTACACCA TGCTCAGCGA GGCCGGGGCC TGTGGACTCT GGGTGGAGGA GAAGGAGCAG
    TGGCTCAACG GGCTGGCCCT GCCTGAACGC CTGGAGGACC TGGAGGTCGT GCAGCAGAGG
    TTCGAGACCC TGGAGCCTGA AATGAACACC CTTGCAGCAC AAATCACCGC GGTGAATGAC
    ATTGCCGAGC AGTTACTGAA GGCCAACCCC CCAGGCAAAG ACCGCATTGT CAACACCCAG
    GAGCAGCTCA ACCACAGGTG GCAGCAGTTT CGGCGTCTGG CAGACGGCAA GAAGGCAGCT
    CTCACCTCAG CCCTGAGCAT CCAGAACTAC CACTTAGAGT GCACGGAGAC CCAGGCCTGG
    ATGAGAGAGA AGACCAAAGT CATCGAGTCC ACCCAGGGCC TAGGCAACGA TCTGGCTGGG
    GTGCTGGCCC TGCAGCGCAA GCTGGCCGGC ACGGAGCGGG ACCTGGAGGC CATCGCCGCC
    CGGGTGGGCG AACTGACTCG AGAGGCAAAT GCCCTGGCTG CCGGCCATCC CGCTCAGGCA
    GTGGCCATCA ACGCCCGGCT GAGAGAGGTG CAGACCGGCT GGGAGGACCT CAGGGCCACC
    ATGCGGCGTC GAGAAGAGTC GCTGGGGGAG GCGCGGCGGC TGCAGGACTT CTTGCGCAGC
    TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACTCAGACTG CTGTGGCCTC TGAAGAAGGG
    CCGGCCACCC TGCCTGAGGC AGAGGCCCTC CTGGCCCAAC ATGCAGCCCT GCGGGGAGAG
    GTGGAGCGGG CCCAGAGCGA GTATAGCCGG CTGCGAGCCC TGGGCGAGGA GGTGACCCGG
    GACCAGGCTG ACCCCCAGTG CCTCTTCCTA CGACAGCGAC TGGAGGCCCT GGGAACTGGC
    TGGGAGGAGC TGGGCCGAAT GTGGGAGAGC CGGCAAGGTC GCCTGGCCCA GGCCCACGGC
    TTCCAGGGAT TCCTGCGGGA TGCTCGTCAG GCTGAGGGCG TGCTCAGCAG CCAGGAATAT
    GTTCTGTCTC ACACGGAGAT GCCAGGGACA CTCCAGGCTG CTGATGCTGC CATTAAAAAA
    CTGGAGGACT TCATGAGCAC CATGGACGCC AATGGGGAAC GGATCCACGG GCTCCTGGAG
    GCTGGCCGCC AGCTGGTATC TGAAGGCAAC ATCCACGCCG ACAAGATTCG GGAAAAGGCA
    GACTCCATTG AGAGGAGGCA CAAGAAGAAT CAAGACGCAG CGCAGCAATT TCTGGGCCGT
    CTTCGGGACA ACCGGGAGCA GCAGCATTTC CTGCAAGATT GTCACGAGCT GAAGCTCTGG
    ATCGACGAGA AGATGCTGAC AGCCCAGGAC GTGTCCTATG ACGAGGCCCG CAACCTGCAT
    ACTAAGTGGC AGAAGCACCA GGCATTCATG GCCGAGCTGG CTGCCAACAA AGACTGGCTG
    GACAAGGTGG ACAAGGCAGT GGGCCAGATT ACCCCGTGGG CTGTATGTAG TCTGCCAATC
    CTTATTTTCG AGTCTTGCCG TCCCTGGTTC TTGGTCTCCC ACCCAGCTGT TCCTCAAGAG
    TCAAGGACAG ATAAAGCTTT ATATCTGCTC AGCAAGTATC CCTACAGAAC ATGGTACGTG
    CTCAACAGTC TGCGAGGCAC TGCAGGGTCT ACGAAAAGAC ACTTTCCTTT TCTCAAGGGC
    ATGATGGTTG GGACAAAAGG AGACCAGTGT GCTTTCAAGT GTTTCCATTC GTTCAGCAAA
    TATTTACTTG GTGCTACTAT ATCCAAAGCA TTGTATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543518.1
    CDS2411..6769
    Translation

    Target ORF information:

    RefSeq Version XM_011545216.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545216.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGAATGGGA ACGGAGTGAG CAGGGTGCAC GGGAACGGCC TGCATGAGGC CAGCGAGTTT 
    TACTATGAGG CTGTGGAAGG GGCCCACAAC CCCGGGGGCC TCCTGCTTTC ACCAGCTGCT
    TTCATCAACC CTGCTCAGTA TGCCAGCGTG CTGGAAGGAC GCTTCAAACA GCTGCAAGAT
    GAACGAGAAG CTGTGCAGAA GAAAACCTTC ACCAAGTGGG TAAACTCGCA CCTGGCCCGG
    GTCACGTGCC GGGTGGGGGA CCTGTACAGC GACCTCCGGG ACGGACGCAA CCTGCTGAGG
    CTCCTCGAGG TGCTCTCGGG AGAGATACTG CCAAAGCCTA CAAAGGGCCG CATGCGGATC
    CACTGCCTGG AGAACGTGGA CAAGGCACTG CAGTTCCTCA AGGAGCAGAA AGTGCACTTG
    GAAAACATGG GCTCCCATGA CATTGTGGAC GGAAACCACC GACTGACCCT TGGGCTGGTC
    TGGACCATCA TCCTTCGATT CCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG
    GAGAAGAAGT CAGCCAAGGA TGCCCTGCTT CTGTGGTGCC AGATGAAGAC TGCAGGTTAT
    CCCAACGTCA ATGTACACAA CTTCACCACC AGCTGGAGAG ATGGACTAGC TTTCAACGCC
    ATCGTGCATA AACACCGGCC AGACCTGCTG GATTTTGAGT CTCTGAAGAA GTGTAATGCA
    CACTATAATC TGCAGAATGC ATTCAATCTG GCTGAAAAGG AACTGGGACT TACCAAGCTG
    CTGGATCCCG AAGACGTGAA TGTGGACCAG CCAGATGAGA AGTCAATCAT TACCTATGTG
    GCTACTTACT ACCATTACTT CTCCAAGATG AAGGCCCTGG CCGTGGAAGG CAAGAGAATT
    GGCAAGGTGC TGGACCATGC CATGGAGGCA GAGCGCCTGG TGGAGAAATA CGAGTCCCTG
    GCCTCGGAGC TGCTGCAGTG GATCGAGCAA ACGATCGTGA CCCTCAATGA CCGGCAGTTG
    GCCAACTCCC TTAGCGGGGT CCAGAACCAG CTGCAGTCCT TCAACTCCTA CCGCACCGTG
    GAGAAGCCGC CCAAGTTTAC CGAGAAAGGG AACTTGGAAG TGCTGCTCTT CACCATCCAG
    AGCAAGCTTC GGGCCAACAA CCAGAAGGTC TACACGCCCC GCGAGGGCCG GCTCATCTCG
    GACATCAACA AGGCTTGGGA GCGGCTGGAG AAGGCGGAGC ACGAGCGTGA GCTGGCCCTG
    CGCACCGAGC TCATCCGCCA GGAGAAGCTG GAGCAGCTGG CCGCCCGCTT CGACCGCAAG
    GCTGCCATGC GGGAGACCTG GCTCAGCGAG AACCAGCGCC TCGTGTCCCA GGACAACTTT
    GGGCTGGAGC TGGCAGCTGT CGAGGCAGCA GTACGGAAGC ACGAAGCCAT TGAGACGGAC
    ATCGTGGCCT ACAGCGGCCG GGTGCAGGCA GTGGACGCCG TGGCTGCAGA GCTGGCCGCC
    GAGCGCTACC ACGACATCAA GCGCATCGCC GCTCGGCAGC ACAACGTGGC ACGGCTCTGG
    GACTTCTTGC GGCAGATGGT GGCCGCCCGG CGGGAGCGGC TCCTCCTCAA CCTGGAGCTG
    CAGAAGGTGT TCCAGGACCT GCTCTACCTC ATGGACTGGA TGGAAGAGAT GAAGGGCCGG
    CTGCAGTCTC AGGACCTGGG CAGGCACCTA GCAGGAGTGG AGGACCTGCT GCAGCTGCAC
    GAGCTGGTGG AGGCAGACAT CGCCGTGCAG GCCGAGAGGG TGCGGGCCGT CAGCGCCTCT
    GCCCTGCGCT TCTGCAACCC AGGGAAAGAG TATAGACCTT GCGACCCGCA GCTGGTGTCG
    GAGCGGGTGG CCAAGCTAGA GCAGAGCTAT GAGGCACTGT GCGAGTTGGC AGCGGCGCGG
    CGGGCCCGGC TGGAGGAATC ACGGCGGCTC TGGCGTTTCC TCTGGGAGGT GGGTGAAGCT
    GAGGCCTGGG TGCGGGAGCA GCAGCACCTC CTGGCCTCAG CCGACACGGG CCGAGACCTG
    ACCGGTGCCC TCCGCCTGCT CAACAAGCAC ACAGCCCTGC GGGGCGAGAT GAGCGGCCGG
    CTGGGGCCCC TGAAGCTCAC CCTGGAGCAG GGCCAGCAGT TGGTGGCCGA GGGTCACCCT
    GGGGCAAGCC AGGCCTCTGC CCGTGCAGCT GAACTCCAAG CCCAGTGGGA GCGGCTAGAG
    GCCCTGGCCG AGGAGCGTGC CCAGCGGCTG GCCCAAGCCG CCAGCCTCTA CCAGTTCCAG
    GCCGATGCAA ACGACATGGA GGCCTGGTTG GTTGACGCAC TGCGCCTGGT GTCCAGCCCC
    GAGCTGGGGC ACGACGAGTT CTCCACGCAG GCTCTAGCCA GGCAGCATCG GGCCCTGGAG
    GAGGAGATTC GAAGCCACCG GCCAACCCTG GACGCCTTGA GGGAACAGGC AGCAGCCCTG
    CCCCCCACAC TGAGCCGCAC GCCCGAGGTG CAGAGCCGGG TGCCCACCCT GGAGCGGCAC
    TACGAGGAGC TGCAGGCCCG GGCAGGCGAG CGAGCGCGGG CCTTGGAGGC AGCCCTGGCG
    CTCTACACCA TGCTCAGCGA GGCCGGGGCC TGTGGACTCT GGGTGGAGGA GAAGGAGCAG
    TGGCTCAACG GGCTGGCCCT GCCTGAACGC CTGGAGGACC TGGAGGTCGT GCAGCAGAGG
    TTCGAGACCC TGGAGCCTGA AATGAACACC CTTGCAGCAC AAATCACCGC GGTGAATGAC
    ATTGCCGAGC AGTTACTGAA GGCCAACCCC CCAGGCAAAG ACCGCATTGT CAACACCCAG
    GAGCAGCTCA ACCACAGGTG GCAGCAGTTT CGGCGTCTGG CAGACGGCAA GAAGGCAGCT
    CTCACCTCAG CCCTGAGCAT CCAGAACTAC CACTTAGAGT GCACGGAGAC CCAGGCCTGG
    ATGAGAGAGA AGACCAAAGT CATCGAGTCC ACCCAGGGCC TAGGCAACGA TCTGGCTGGG
    GTGCTGGCCC TGCAGCGCAA GCTGGCCGGC ACGGAGCGGG ACCTGGAGGC CATCGCCGCC
    CGGGTGGGCG AACTGACTCG AGAGGCAAAT GCCCTGGCTG CCGGCCATCC CGCTCAGGCA
    GTGGCCATCA ACGCCCGGCT GAGAGAGGTG CAGACCGGCT GGGAGGACCT CAGGGCCACC
    ATGCGGCGTC GAGAAGAGTC GCTGGGGGAG GCGCGGCGGC TGCAGGACTT CTTGCGCAGC
    TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACTCAGACTG CTGTGGCCTC TGAAGAAGGG
    CCGGCCACCC TGCCTGAGGC AGAGGCCCTC CTGGCCCAAC ATGCAGCCCT GCGGGGAGAG
    GTGGAGCGGG CCCAGAGCGA GTATAGCCGG CTGCGAGCCC TGGGCGAGGA GGTGACCCGG
    GACCAGGCTG ACCCCCAGTG CCTCTTCCTA CGACAGCGAC TGGAGGCCCT GGGAACTGGC
    TGGGAGGAGC TGGGCCGAAT GTGGGAGAGC CGGCAAGGTC GCCTGGCCCA GGCCCACGGC
    TTCCAGGGAT TCCTGCGGGA TGCTCGTCAG GCTGAGGGCG TGCTCAGCAG CCAGGAATAT
    GTTCTGTCTC ACACGGAGAT GCCAGGGACA CTCCAGGCTG CTGATGCTGC CATTAAAAAA
    CTGGAGGACT TCATGAGCAC CATGGACGCC AATGGGGAAC GGATCCACGG GCTCCTGGAG
    GCTGGCCGCC AGCTGGTATC TGAAGGCAAC ATCCACGCCG ACAAGATTCG GGAAAAGGCA
    GACTCCATTG AGAGGAGGCA CAAGAAGAAT CAAGACGCAG CGCAGCAATT TCTGGGCCGT
    CTTCGGGACA ACCGGGAGCA GCAGCATTTC CTGCAAGATT GTCACGAGCT GAAGCTCTGG
    ATCGACGAGA AGATGCTGAC AGCCCAGGAC GTGTCCTATG ACGAGGCCCG CAACCTGCAT
    ACTAAGTGGC AGAAGCACCA GGCATTCATG GCCGAGCTGG CTGCCAACAA AGACTGGCTG
    GACAAGGTGG ACAAGGCAGT GGGCCAGATT ACCCCGTGGG CTGTATGTAG TCTGCCAATC
    CTTATTTTCG AGTCTTGCCG TCCCTGGTTC TTGGTCTCCC ACCCAGCTGT TCCTCAAGAG
    TCAAGGACAG ATAAAGCTTT ATATCTGCTC AGCAAGTATC CCTACAGAAC ATGGTACGTG
    CTCAACAGTC TGCGAGGCAC TGCAGGGTCT ACGAAAAGAC ACTTTCCTTT TCTCAAGGGC
    ATGATGGTTG GGACAAAAGG AGACCAGTGT GCTTTCAAGT GTTTCCATTC GTTCAGCAAA
    TATTTACTTG GTGCTACTAT ATCCAAAGCA TTGTATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60721
    Clone ID Related Accession (Same CDS sequence) XM_011545217.2
    Accession Version XM_011545217.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011545217.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGGGA ACGGAGTGAG CAGGGTGCAC GGGAACGGCC TGCATGAGGC CAGCGAGTTT 
    TACTATGAGG CTGTGGAAGG GGCCCACAAC CCCGGGGGCC TCCTGCTTTC ACCAGCTGCT
    TTCATCAACC CTGCTCAGTA TGCCAGCGTG CTGGAAGGAC GCTTCAAACA GCTGCAAGAT
    GAACGAGAAG CTGTGCAGAA GAAAACCTTC ACCAAGTGGG TAAACTCGCA CCTGGCCCGG
    GTCACGTGCC GGGTGGGGGA CCTGTACAGC GACCTCCGGG ACGGACGCAA CCTGCTGAGG
    CTCCTCGAGG TGCTCTCGGG AGAGATACTG CCAAAGCCTA CAAAGGGCCG CATGCGGATC
    CACTGCCTGG AGAACGTGGA CAAGGCACTG CAGTTCCTCA AGGAGCAGAA AGTGCACTTG
    GAAAACATGG GCTCCCATGA CATTGTGGAC GGAAACCACC GACTGACCCT TGGGCTGGTC
    TGGACCATCA TCCTTCGATT CCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG
    GAGAAGAAGT CAGCCAAGGA TGCCCTGCTT CTGTGGTGCC AGATGAAGAC TGCAGGTTAT
    CCCAACGTCA ATGTACACAA CTTCACCACC AGCTGGAGAG ATGGACTAGC TTTCAACGCC
    ATCGTGCATA AACACCGGCC AGACCTGCTG GATTTTGAGT CTCTGAAGAA GTGTAATGCA
    CACTATAATC TGCAGAATGC ATTCAATCTG GCTGAAAAGG AACTGGGACT TACCAAGCTG
    CTGGATCCCG AAGACGTGAA TGTGGACCAG CCAGATGAGA AGTCAATCAT TACCTATGTG
    GCTACTTACT ACCATTACTT CTCCAAGATG AAGGCCCTGG CCGTGGAAGG CAAGAGAATT
    GGCAAGGTGC TGGACCATGC CATGGAGGCA GAGCGCCTGG TGGAGAAATA CGAGTCCCTG
    GCCTCGGAGC TGCTGCAGTG GATCGAGCAA ACGATCGTGA CCCTCAATGA CCGGCAGTTG
    GCCAACTCCC TTAGCGGGGT CCAGAACCAG CTGCAGTCCT TCAACTCCTA CCGCACCGTG
    GAGAAGCCGC CCAAGTTTAC CGAGAAAGGG AACTTGGAAG TGCTGCTCTT CACCATCCAG
    AGCAAGCTTC GGGCCAACAA CCAGAAGGTC TACACGCCCC GCGAGGGCCG GCTCATCTCG
    GACATCAACA AGGCTTGGGA GCGGCTGGAG AAGGCGGAGC ACGAGCGTGA GCTGGCCCTG
    CGCACCGAGC TCATCCGCCA GGAGAAGCTG GAGCAGCTGG CCGCCCGCTT CGACCGCAAG
    GCTGCCATGC GGGAGACCTG GCTCAGCGAG AACCAGCGCC TCGTGTCCCA GGACAACTTT
    GGGCTGGAGC TGGCAGCTGT CGAGGCAGCA GTACGGAAGC ACGAAGCCAT TGAGACGGAC
    ATCGTGGCCT ACAGCGGCCG GGTGCAGGCA GTGGACGCCG TGGCTGCAGA GCTGGCCGCC
    GAGCGCTACC ACGACATCAA GCGCATCGCC GCTCGGCAGC ACAACGTGGC ACGGCTCTGG
    GACTTCTTGC GGCAGATGGT GGCCGCCCGG CGGGAGCGGC TCCTCCTCAA CCTGGAGCTG
    CAGAAGGTGT TCCAGGACCT GCTCTACCTC ATGGACTGGA TGGAAGAGAT GAAGGGCCGG
    CTGCAGTCTC AGGACCTGGG CAGGCACCTA GCAGGAGTGG AGGACCTGCT GCAGCTGCAC
    GAGCTGGTGG AGGCAGACAT CGCCGTGCAG GCCGAGAGGG TGCGGGCCGT CAGCGCCTCT
    GCCCTGCGCT TCTGCAACCC AGGGAAAGAG TATAGACCTT GCGACCCGCA GCTGGTGTCG
    GAGCGGGTGG CCAAGCTAGA GCAGAGCTAT GAGGCACTGT GCGAGTTGGC AGCGGCGCGG
    CGGGCCCGGC TGGAGGAATC ACGGCGGCTC TGGCGTTTCC TCTGGGAGGT GGGTGAAGCT
    GAGGCCTGGG TGCGGGAGCA GCAGCACCTC CTGGCCTCAG CCGACACGGG CCGAGACCTG
    ACCGGTGCCC TCCGCCTGCT CAACAAGCAC ACAGCCCTGC GGGGCGAGAT GAGCGGCCGG
    CTGGGGCCCC TGAAGCTCAC CCTGGAGCAG GGCCAGCAGT TGGTGGCCGA GGGTCACCCT
    GGGGCAAGCC AGGCCTCTGC CCGTGCAGCT GAACTCCAAG CCCAGTGGGA GCGGCTAGAG
    GCCCTGGCCG AGGAGCGTGC CCAGCGGCTG GCCCAAGCCG CCAGCCTCTA CCAGTTCCAG
    GCCGATGCAA ACGACATGGA GGCCTGGTTG GTTGACGCAC TGCGCCTGGT GTCCAGCCCC
    GAGCTGGGGC ACGACGAGTT CTCCACGCAG GCTCTAGCCA GGCAGCATCG GGCCCTGGAG
    GAGGAGATTC GAAGCCACCG GCCAACCCTG GACGCCTTGA GGGAACAGGC AGCAGCCCTG
    CCCCCCACAC TGAGCCGCAC GCCCGAGGTG CAGAGCCGGG TGCCCACCCT GGAGCGGCAC
    TACGAGGAGC TGCAGGCCCG GGCAGGCGAG CGAGCGCGGG CCTTGGAGGC AGCCCTGGCG
    CTCTACACCA TGCTCAGCGA GGCCGGGGCC TGTGGACTCT GGGTGGAGGA GAAGGAGCAG
    TGGCTCAACG GGCTGGCCCT GCCTGAACGC CTGGAGGACC TGGAGGTCGT GCAGCAGAGG
    TTCGAGACCC TGGAGCCTGA AATGAACACC CTTGCAGCAC AAATCACCGC GGTGAATGAC
    ATTGCCGAGC AGTTACTGAA GGCCAACCCC CCAGGCAAAG ACCGCATTGT CAACACCCAG
    GAGCAGCTCA ACCACAGGTG GCAGCAGTTT CGGCGTCTGG CAGACGGCAA GAAGGCAGCT
    CTCACCTCAG CCCTGAGCAT CCAGAACTAC CACTTAGAGT GCACGGAGAC CCAGGCCTGG
    ATGAGAGAGA AGACCAAAGT CATCGAGTCC ACCCAGGGCC TAGGCAACGA TCTGGCTGGG
    GTGCTGGCCC TGCAGCGCAA GCTGGCCGGC ACGGAGCGGG ACCTGGAGGC CATCGCCGCC
    CGGGTGGGCG AACTGACTCG AGAGGCAAAT GCCCTGGCTG CCGGCCATCC CGCTCAGGCA
    GTGGCCATCA ACGCCCGGCT GAGAGAGGTG CAGACCGGCT GGGAGGACCT CAGGGCCACC
    ATGCGGCGTC GAGAAGAGTC GCTGGGGGAG GCGCGGCGGC TGCAGGACTT CTTGCGCAGC
    TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACTCAGACTG CTGTGGCCTC TGAAGAAGGG
    CCGGCCACCC TGCCTGAGGC AGAGGCCCTC CTGGCCCAAC ATGCAGCCCT GCGGGGAGAG
    GTGGAGCGGG CCCAGAGCGA GTATAGCCGG CTGCGAGCCC TGGGCGAGGA GGTGACCCGG
    GACCAGGCTG ACCCCCAGTG CCTCTTCCTA CGACAGCGAC TGGAGGCCCT GGGAACTGGC
    TGGGAGGAGC TGGGCCGAAT GTGGGAGAGC CGGCAAGGTC GCCTGGCCCA GGCCCACGGC
    TTCCAGGGAT TCCTGCGGGA TGCTCGTCAG GCTGAGGGCG TGCTCAGCAG CCAGGAATAT
    GTTCTGTCTC ACACGGAGAT GCCAGGGACA CTCCAGGCTG CTGATGCTGC CATTAAAAAA
    CTGGAGGACT TCATGAGCAC CATGGACGCC AATGGGGAAC GGATCCACGG GCTCCTGGAG
    GCTGGCCGCC AGCTGGTATC TGAAGGCAAC ATCCACGCCG ACAAGATTCG GGAAAAGGCA
    GACTCCATTG AGAGGAGGCA CAAGAAGAAT CAAGACGCAG CGCAGCAATT TCTGGGCCGT
    CTTCGGGACA ACCGGGAGCA GCAGCATTTC CTGCAAGATT GTCACGAGCT GAAGCTCTGG
    ATCGACGAGA AGATGCTGAC AGCCCAGGAC GTGTCCTATG ACGAGGCCCG CAACCTGCAT
    ACTAAGTGGC AGAAGCACCA GGCATTCATG GCCGAGCTGG CTGCCAACAA AGACTGGCTG
    GACAAGGTGG ACAAGGCAGT GGGCCAGATT ACCCCGTGGG CTGTATGTAG TCTGCCAATC
    CTTATTTTCG AGTCTTGCCG TCCCTGGTTC TTGGTCTCCC ACCCAGCTGT TCCTCAAGAG
    TCAAGGACAG ATAAAGCTTT ATATCTGCTC AGCAAGTATC CCTACAGAAC ATGTTCAAGA
    GAGCACACTT CCCTCACCAC CCCAGACACA GAAATCCTCA AGCAGAAAAG CTGGAAGTGG
    GGAAACCAGG TGGGACTCAA CTTGCTATCA CTGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011543519.1
    CDS2411..6709
    Translation

    Target ORF information:

    RefSeq Version XM_011545217.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011545217.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAATGGGA ACGGAGTGAG CAGGGTGCAC GGGAACGGCC TGCATGAGGC CAGCGAGTTT 
    TACTATGAGG CTGTGGAAGG GGCCCACAAC CCCGGGGGCC TCCTGCTTTC ACCAGCTGCT
    TTCATCAACC CTGCTCAGTA TGCCAGCGTG CTGGAAGGAC GCTTCAAACA GCTGCAAGAT
    GAACGAGAAG CTGTGCAGAA GAAAACCTTC ACCAAGTGGG TAAACTCGCA CCTGGCCCGG
    GTCACGTGCC GGGTGGGGGA CCTGTACAGC GACCTCCGGG ACGGACGCAA CCTGCTGAGG
    CTCCTCGAGG TGCTCTCGGG AGAGATACTG CCAAAGCCTA CAAAGGGCCG CATGCGGATC
    CACTGCCTGG AGAACGTGGA CAAGGCACTG CAGTTCCTCA AGGAGCAGAA AGTGCACTTG
    GAAAACATGG GCTCCCATGA CATTGTGGAC GGAAACCACC GACTGACCCT TGGGCTGGTC
    TGGACCATCA TCCTTCGATT CCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG
    GAGAAGAAGT CAGCCAAGGA TGCCCTGCTT CTGTGGTGCC AGATGAAGAC TGCAGGTTAT
    CCCAACGTCA ATGTACACAA CTTCACCACC AGCTGGAGAG ATGGACTAGC TTTCAACGCC
    ATCGTGCATA AACACCGGCC AGACCTGCTG GATTTTGAGT CTCTGAAGAA GTGTAATGCA
    CACTATAATC TGCAGAATGC ATTCAATCTG GCTGAAAAGG AACTGGGACT TACCAAGCTG
    CTGGATCCCG AAGACGTGAA TGTGGACCAG CCAGATGAGA AGTCAATCAT TACCTATGTG
    GCTACTTACT ACCATTACTT CTCCAAGATG AAGGCCCTGG CCGTGGAAGG CAAGAGAATT
    GGCAAGGTGC TGGACCATGC CATGGAGGCA GAGCGCCTGG TGGAGAAATA CGAGTCCCTG
    GCCTCGGAGC TGCTGCAGTG GATCGAGCAA ACGATCGTGA CCCTCAATGA CCGGCAGTTG
    GCCAACTCCC TTAGCGGGGT CCAGAACCAG CTGCAGTCCT TCAACTCCTA CCGCACCGTG
    GAGAAGCCGC CCAAGTTTAC CGAGAAAGGG AACTTGGAAG TGCTGCTCTT CACCATCCAG
    AGCAAGCTTC GGGCCAACAA CCAGAAGGTC TACACGCCCC GCGAGGGCCG GCTCATCTCG
    GACATCAACA AGGCTTGGGA GCGGCTGGAG AAGGCGGAGC ACGAGCGTGA GCTGGCCCTG
    CGCACCGAGC TCATCCGCCA GGAGAAGCTG GAGCAGCTGG CCGCCCGCTT CGACCGCAAG
    GCTGCCATGC GGGAGACCTG GCTCAGCGAG AACCAGCGCC TCGTGTCCCA GGACAACTTT
    GGGCTGGAGC TGGCAGCTGT CGAGGCAGCA GTACGGAAGC ACGAAGCCAT TGAGACGGAC
    ATCGTGGCCT ACAGCGGCCG GGTGCAGGCA GTGGACGCCG TGGCTGCAGA GCTGGCCGCC
    GAGCGCTACC ACGACATCAA GCGCATCGCC GCTCGGCAGC ACAACGTGGC ACGGCTCTGG
    GACTTCTTGC GGCAGATGGT GGCCGCCCGG CGGGAGCGGC TCCTCCTCAA CCTGGAGCTG
    CAGAAGGTGT TCCAGGACCT GCTCTACCTC ATGGACTGGA TGGAAGAGAT GAAGGGCCGG
    CTGCAGTCTC AGGACCTGGG CAGGCACCTA GCAGGAGTGG AGGACCTGCT GCAGCTGCAC
    GAGCTGGTGG AGGCAGACAT CGCCGTGCAG GCCGAGAGGG TGCGGGCCGT CAGCGCCTCT
    GCCCTGCGCT TCTGCAACCC AGGGAAAGAG TATAGACCTT GCGACCCGCA GCTGGTGTCG
    GAGCGGGTGG CCAAGCTAGA GCAGAGCTAT GAGGCACTGT GCGAGTTGGC AGCGGCGCGG
    CGGGCCCGGC TGGAGGAATC ACGGCGGCTC TGGCGTTTCC TCTGGGAGGT GGGTGAAGCT
    GAGGCCTGGG TGCGGGAGCA GCAGCACCTC CTGGCCTCAG CCGACACGGG CCGAGACCTG
    ACCGGTGCCC TCCGCCTGCT CAACAAGCAC ACAGCCCTGC GGGGCGAGAT GAGCGGCCGG
    CTGGGGCCCC TGAAGCTCAC CCTGGAGCAG GGCCAGCAGT TGGTGGCCGA GGGTCACCCT
    GGGGCAAGCC AGGCCTCTGC CCGTGCAGCT GAACTCCAAG CCCAGTGGGA GCGGCTAGAG
    GCCCTGGCCG AGGAGCGTGC CCAGCGGCTG GCCCAAGCCG CCAGCCTCTA CCAGTTCCAG
    GCCGATGCAA ACGACATGGA GGCCTGGTTG GTTGACGCAC TGCGCCTGGT GTCCAGCCCC
    GAGCTGGGGC ACGACGAGTT CTCCACGCAG GCTCTAGCCA GGCAGCATCG GGCCCTGGAG
    GAGGAGATTC GAAGCCACCG GCCAACCCTG GACGCCTTGA GGGAACAGGC AGCAGCCCTG
    CCCCCCACAC TGAGCCGCAC GCCCGAGGTG CAGAGCCGGG TGCCCACCCT GGAGCGGCAC
    TACGAGGAGC TGCAGGCCCG GGCAGGCGAG CGAGCGCGGG CCTTGGAGGC AGCCCTGGCG
    CTCTACACCA TGCTCAGCGA GGCCGGGGCC TGTGGACTCT GGGTGGAGGA GAAGGAGCAG
    TGGCTCAACG GGCTGGCCCT GCCTGAACGC CTGGAGGACC TGGAGGTCGT GCAGCAGAGG
    TTCGAGACCC TGGAGCCTGA AATGAACACC CTTGCAGCAC AAATCACCGC GGTGAATGAC
    ATTGCCGAGC AGTTACTGAA GGCCAACCCC CCAGGCAAAG ACCGCATTGT CAACACCCAG
    GAGCAGCTCA ACCACAGGTG GCAGCAGTTT CGGCGTCTGG CAGACGGCAA GAAGGCAGCT
    CTCACCTCAG CCCTGAGCAT CCAGAACTAC CACTTAGAGT GCACGGAGAC CCAGGCCTGG
    ATGAGAGAGA AGACCAAAGT CATCGAGTCC ACCCAGGGCC TAGGCAACGA TCTGGCTGGG
    GTGCTGGCCC TGCAGCGCAA GCTGGCCGGC ACGGAGCGGG ACCTGGAGGC CATCGCCGCC
    CGGGTGGGCG AACTGACTCG AGAGGCAAAT GCCCTGGCTG CCGGCCATCC CGCTCAGGCA
    GTGGCCATCA ACGCCCGGCT GAGAGAGGTG CAGACCGGCT GGGAGGACCT CAGGGCCACC
    ATGCGGCGTC GAGAAGAGTC GCTGGGGGAG GCGCGGCGGC TGCAGGACTT CTTGCGCAGC
    TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACTCAGACTG CTGTGGCCTC TGAAGAAGGG
    CCGGCCACCC TGCCTGAGGC AGAGGCCCTC CTGGCCCAAC ATGCAGCCCT GCGGGGAGAG
    GTGGAGCGGG CCCAGAGCGA GTATAGCCGG CTGCGAGCCC TGGGCGAGGA GGTGACCCGG
    GACCAGGCTG ACCCCCAGTG CCTCTTCCTA CGACAGCGAC TGGAGGCCCT GGGAACTGGC
    TGGGAGGAGC TGGGCCGAAT GTGGGAGAGC CGGCAAGGTC GCCTGGCCCA GGCCCACGGC
    TTCCAGGGAT TCCTGCGGGA TGCTCGTCAG GCTGAGGGCG TGCTCAGCAG CCAGGAATAT
    GTTCTGTCTC ACACGGAGAT GCCAGGGACA CTCCAGGCTG CTGATGCTGC CATTAAAAAA
    CTGGAGGACT TCATGAGCAC CATGGACGCC AATGGGGAAC GGATCCACGG GCTCCTGGAG
    GCTGGCCGCC AGCTGGTATC TGAAGGCAAC ATCCACGCCG ACAAGATTCG GGAAAAGGCA
    GACTCCATTG AGAGGAGGCA CAAGAAGAAT CAAGACGCAG CGCAGCAATT TCTGGGCCGT
    CTTCGGGACA ACCGGGAGCA GCAGCATTTC CTGCAAGATT GTCACGAGCT GAAGCTCTGG
    ATCGACGAGA AGATGCTGAC AGCCCAGGAC GTGTCCTATG ACGAGGCCCG CAACCTGCAT
    ACTAAGTGGC AGAAGCACCA GGCATTCATG GCCGAGCTGG CTGCCAACAA AGACTGGCTG
    GACAAGGTGG ACAAGGCAGT GGGCCAGATT ACCCCGTGGG CTGTATGTAG TCTGCCAATC
    CTTATTTTCG AGTCTTGCCG TCCCTGGTTC TTGGTCTCCC ACCCAGCTGT TCCTCAAGAG
    TCAAGGACAG ATAAAGCTTT ATATCTGCTC AGCAAGTATC CCTACAGAAC ATGTTCAAGA
    GAGCACACTT CCCTCACCAC CCCAGACACA GAAATCCTCA AGCAGAAAAG CTGGAAGTGG
    GGAAACCAGG TGGGACTCAA CTTGCTATCA CTGAAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45471
    Clone ID Related Accession (Same CDS sequence) XM_017018176.1 , XM_005274193.3 , XM_017018175.1 , XM_017018177.1 , XM_017018174.1 , XM_017018175.2 , XM_006718671.4 , XM_017018177.2 , XM_006718671.3 , XM_005274192.4 , XM_017018178.1 , NM_006946.4
    Accession Version NM_006946.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-16
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AP001157.4 and AB008567.1. On or before Dec 17, 2019 this sequence version replaced XM_005274192.4, NM_006946.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AB008567.1 [ECO:0000331] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_008877.2
    CDS590..7762
    Translation

    Target ORF information:

    RefSeq Version NM_006946.4
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006946.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27129
    Clone ID Related Accession (Same CDS sequence) NM_006946.2 , NM_006946.3
    Accession Version NM_006946.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-10-05
    Organism Homo sapiens(Human)
    Product spectrin beta chain, non-erythrocytic 2
    Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB008567.1 and AF026487.1. This sequence is a reference standard in the RefSeqGene project. On Aug 29, 2008 this sequence version replaced NM_006946.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB008567.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGGGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_008877.1
    CDS73..7245
    Translation

    Target ORF information:

    RefSeq Version NM_006946.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006946.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGGGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu27129
    Clone ID Related Accession (Same CDS sequence) NM_006946.2 , NM_006946.3
    Accession Version NM_006946.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB008567.1, AF026487.1 and AP001157.4. This sequence is a reference standard in the RefSeqGene project. On Aug 24, 2017 this sequence version replaced NM_006946.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB008567.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGGGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_008877.1
    CDS73..7245
    Translation

    Target ORF information:

    RefSeq Version NM_006946.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_006946.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGGGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45470
    Clone ID Related Accession (Same CDS sequence) XM_006718669.3
    Accession Version XM_006718669.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7194bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718669.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATGGGA ACGGAGTGAG CAGGGTGCAC GGGAACGGCC TGCATGAGGC CAGCGAGTTT 
    TACTATGAGG CTGTGGAAGG GGCCCACAAC CCCGGGGGCC TCCTGCTTTC ACCAGCTGCT
    TTCATCAACC CTGCTCAGTA TGCCAGCGTG CTGGAAGGAC GCTTCAAACA GCTGCAAGAT
    GAACGAGAAG CTGTGCAGAA GAAAACCTTC ACCAAGTGGG TAAACTCGCA CCTGGCCCGG
    GTCACGTGCC GGGTGGGGGA CCTGTACAGC GACCTCCGGG ACGGACGCAA CCTGCTGAGG
    CTCCTCGAGG TGCTCTCGGG AGAGATACTG CCAAAGCCTA CAAAGGGCCG CATGCGGATC
    CACTGCCTGG AGAACGTGGA CAAGGCACTG CAGTTCCTCA AGGAGCAGAA AGTGCACTTG
    GAAAACATGG GCTCCCATGA CATTGTGGAC GGAAACCACC GACTGACCCT TGGGCTGGTC
    TGGACCATCA TCCTTCGATT CCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG
    GAGAAGAAGT CAGCCAAGGA TGCCCTGCTT CTGTGGTGCC AGATGAAGAC TGCAGGTTAT
    CCCAACGTCA ATGTACACAA CTTCACCACC AGCTGGAGAG ATGGACTAGC TTTCAACGCC
    ATCGTGCATA AACACCGGCC AGACCTGCTG GATTTTGAGT CTCTGAAGAA GTGTAATGCA
    CACTATAATC TGCAGAATGC ATTCAATCTG GCTGAAAAGG AACTGGGACT TACCAAGCTG
    CTGGATCCCG AAGACGTGAA TGTGGACCAG CCAGATGAGA AGTCAATCAT TACCTATGTG
    GCTACTTACT ACCATTACTT CTCCAAGATG AAGGCCCTGG CCGTGGAAGG CAAGAGAATT
    GGCAAGGTGC TGGACCATGC CATGGAGGCA GAGCGCCTGG TGGAGAAATA CGAGTCCCTG
    GCCTCGGAGC TGCTGCAGTG GATCGAGCAA ACGATCGTGA CCCTCAATGA CCGGCAGTTG
    GCCAACTCCC TTAGCGGGGT CCAGAACCAG CTGCAGTCCT TCAACTCCTA CCGCACCGTG
    GAGAAGCCGC CCAAGTTTAC CGAGAAAGGG AACTTGGAAG TGCTGCTCTT CACCATCCAG
    AGCAAGCTTC GGGCCAACAA CCAGAAGGTC TACACGCCCC GCGAGGGCCG GCTCATCTCG
    GACATCAACA AGGCTTGGGA GCGGCTGGAG AAGGCGGAGC ACGAGCGTGA GCTGGCCCTG
    CGCACCGAGC TCATCCGCCA GGAGAAGCTG GAGCAGCTGG CCGCCCGCTT CGACCGCAAG
    GCTGCCATGC GGGAGACCTG GCTCAGCGAG AACCAGCGCC TCGTGTCCCA GGACAACTTT
    GGGCTGGAGC TGGCAGCTGT CGAGGCAGCA GTACGGAAGC ACGAAGCCAT TGAGACGGAC
    ATCGTGGCCT ACAGCGGCCG GGTGCAGGCA GTGGACGCCG TGGCTGCAGA GCTGGCCGCC
    GAGCGCTACC ACGACATCAA GCGCATCGCC GCTCGGCAGC ACAACGTGGC ACGGCTCTGG
    GACTTCTTGC GGCAGATGGT GGCCGCCCGG CGGGAGCGGC TCCTCCTCAA CCTGGAGCTG
    CAGAAGGTGT TCCAGGACCT GCTCTACCTC ATGGACTGGA TGGAAGAGAT GAAGGGCCGG
    CTGCAGTCTC AGGACCTGGG CAGGCACCTA GCAGGAGTGG AGGACCTGCT GCAGCTGCAC
    GAGCTGGTGG AGGCAGACAT CGCCGTGCAG GCCGAGAGGG TGCGGGCCGT CAGCGCCTCT
    GCCCTGCGCT TCTGCAACCC AGGGAAAGAG TATAGACCTT GCGACCCGCA GCTGGTGTCG
    GAGCGGGTGG CCAAGCTAGA GCAGAGCTAT GAGGCACTGT GCGAGTTGGC AGCGGCGCGG
    CGGGCCCGGC TGGAGGAATC ACGGCGGCTC TGGCGTTTCC TCTGGGAGGT GGGTGAAGCT
    GAGGCCTGGG TGCGGGAGCA GCAGCACCTC CTGGCCTCAG CCGACACGGG CCGAGACCTG
    ACCGGTGCCC TCCGCCTGCT CAACAAGCAC ACAGCCCTGC GGGGCGAGAT GAGCGGCCGG
    CTGGGGCCCC TGAAGCTCAC CCTGGAGCAG GGCCAGCAGT TGGTGGCCGA GGGTCACCCT
    GGGGCAAGCC AGGCCTCTGC CCGTGCAGCT GAACTCCAAG CCCAGTGGGA GCGGCTAGAG
    GCCCTGGCCG AGGAGCGTGC CCAGCGGCTG GCCCAAGCCG CCAGCCTCTA CCAGTTCCAG
    GCCGATGCAA ACGACATGGA GGCCTGGTTG GTTGACGCAC TGCGCCTGGT GTCCAGCCCC
    GAGCTGGGGC ACGACGAGTT CTCCACGCAG GCTCTAGCCA GGCAGCATCG GGCCCTGGAG
    GAGGAGATTC GAAGCCACCG GCCAACCCTG GACGCCTTGA GGGAACAGGC AGCAGCCCTG
    CCCCCCACAC TGAGCCGCAC GCCCGAGGTG CAGAGCCGGG TGCCCACCCT GGAGCGGCAC
    TACGAGGAGC TGCAGGCCCG GGCAGGCGAG CGAGCGCGGG CCTTGGAGGC AGCCCTGGCG
    CTCTACACCA TGCTCAGCGA GGCCGGGGCC TGTGGACTCT GGGTGGAGGA GAAGGAGCAG
    TGGCTCAACG GGCTGGCCCT GCCTGAACGC CTGGAGGACC TGGAGGTCGT GCAGCAGAGG
    TTCGAGACCC TGGAGCCTGA AATGAACACC CTTGCAGCAC AAATCACCGC GGTGAATGAC
    ATTGCCGAGC AGTTACTGAA GGCCAACCCC CCAGGCAAAG ACCGCATTGT CAACACCCAG
    GAGCAGCTCA ACCACAGGTG GCAGCAGTTT CGGCGTCTGG CAGACGGCAA GAAGGCAGCT
    CTCACCTCAG CCCTGAGCAT CCAGAACTAC CACTTAGAGT GCACGGAGAC CCAGGCCTGG
    ATGAGAGAGA AGACCAAAGT CATCGAGTCC ACCCAGGGCC TAGGCAACGA TCTGGCTGGG
    GTGCTGGCCC TGCAGCGCAA GCTGGCCGGC ACGGAGCGGG ACCTGGAGGC CATCGCCGCC
    CGGGTGGGCG AACTGACTCG AGAGGCAAAT GCCCTGGCTG CCGGCCATCC CGCTCAGGCA
    GTGGCCATCA ACGCCCGGCT GAGAGAGGTG CAGACCGGCT GGGAGGACCT CAGGGCCACC
    ATGCGGCGTC GAGAAGAGTC GCTGGGGGAG GCGCGGCGGC TGCAGGACTT CTTGCGCAGC
    TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACTCAGACTG CTGTGGCCTC TGAAGAAGGG
    CCGGCCACCC TGCCTGAGGC AGAGGCCCTC CTGGCCCAAC ATGCAGCCCT GCGGGGAGAG
    GTGGAGCGGG CCCAGAGCGA GTATAGCCGG CTGCGAGCCC TGGGCGAGGA GGTGACCCGG
    GACCAGGCTG ACCCCCAGTG CCTCTTCCTA CGACAGCGAC TGGAGGCCCT GGGAACTGGC
    TGGGAGGAGC TGGGCCGAAT GTGGGAGAGC CGGCAAGGTC GCCTGGCCCA GGCCCACGGC
    TTCCAGGGAT TCCTGCGGGA TGCTCGTCAG GCTGAGGGCG TGCTCAGCAG CCAGGAATAT
    GTTCTGTCTC ACACGGAGAT GCCAGGGACA CTCCAGGCTG CTGATGCTGC CATTAAAAAA
    CTGGAGGACT TCATGAGCAC CATGGACGCC AATGGGGAAC GGATCCACGG GCTCCTGGAG
    GCTGGCCGCC AGCTGGTATC TGAAGGCAAC ATCCACGCCG ACAAGATTCG GGAAAAGGCA
    GACTCCATTG AGAGGAGGCA CAAGAAGAAT CAAGACGCAG CGCAGCAATT TCTGGGCCGT
    CTTCGGGACA ACCGGGAGCA GCAGCATTTC CTGCAAGATT GTCACGAGCT GAAGCTCTGG
    ATCGACGAGA AGATGCTGAC AGCCCAGGAC GTGTCCTATG ACGAGGCCCG CAACCTGCAT
    ACTAAGTGGC AGAAGCACCA GGCATTCATG GCCGAGCTGG CTGCCAACAA AGACTGGCTG
    GACAAGGTGG ACAAGGAAGG GCGAGAGCTC ACCCTTGAGA AGCCAGAGCT GAAAGCCCTG
    GTGTCGGAGA AGCTGAGAGA CCTGCACAGG CGCTGGGACG AGCTGGAGAC CACCACCCAA
    GCCAAGGCCC GCAGCCTCTT TGATGCCAAC CGAGCTGAGC TGTTTGCCCA GAGCTGCTGT
    GCCCTGGAGA GCTGGCTGGA GAGCCTGCAG GCCCAGCTGC ACTCGGATGA CTACGGCAAG
    GACCTCACCA GCGTCAACAT CCTGCTCAAG AAGCAGCAGA TGCTGGAATG GGAGATGGCT
    GTGAGAGAGA AGGAGGTGGA GGCAATCCAG GCCCAGGCCA AAGCACTGGC CCAGGAGGAC
    CAGGGTGCAG GGGAGGTGGA GAGAACCTCG AGGGCCGTGG AGGAGAAGTT CAGGGCCTTG
    TGCCAGCCCA TGCGGGAACG CTGCCGGCGC CTGCAGGCTT CTCGCGAGCA GCACCAGTTC
    CACCGCGATG TGGAAGATGA GATTTTGTGG GTGACAGAGC GGCTGCCCAT GGCCAGCTCC
    ATGGAGCATG GCAAGGACCT GCCCAGCGTC CAGCTTCTCA TGAAGAAAAA CCAGACCCTG
    CAGAAAGAGA TTCAGGGCCA TGAGCCCCGG ATCGCGGACC TGAGGGAGCG GCAGCGTGCT
    CTAGGTGCAG CAGCAGCAGG TCCAGAGCTG GCTGAGCTGC AGGAAATGTG GAAACGCCTG
    GGCCACGAGC TGGAACTTCG AGGGAAGCGA CTGGAGGATG CCCTGCGAGC CCAGCAGTTC
    TACCGCGATG CCGCCGAGGC GGAGGCCTGG ATGGGCGAGC AGGAATTACA CATGATGGGC
    CAGGAGAAGG CCAAGGATGA GCTGAGTGCC CAGGCAGAGG TGAAGAAGCA CCAGGTGCTG
    GAGCAAGCCC TGGCCGACTA CGCGCAGACC ATCCACCAGC TGGCGGCCAG CAGCCAGGAC
    ATGATTGACC ACGAGCACCC AGAGAGCACT CGGATATCCA TCCGCCAAGC CCAGGTGGAC
    AAGCTGTATG CCGGCCTGAA GGAGCTGGCT GGAGAGCGGC GGGAGCGCCT GCAGGAGCAC
    CTCCGGCTGT GCCAGCTCCG CCGCGAGCTG GATGACCTGG AACAGTGGAT CCAGGAGCGC
    GAGGTGGTGG CGGCCTCCCA CGAGCTGGGC CAGGACTACG AGCATGTGAC TATGCTCCGA
    GACAAATTCC GAGAGTTCTC CCGGGACACA AGCACCATCG GTCAGGAGCG CGTAGATAGC
    GCCAATGCGC TGGCCAATGG GCTCATTGCT GGGGGCCATG CTGCACGGGC CACCGTGGCC
    GAGTGGAAGG ACAGTCTCAA CGAGGCCTGG GCTGACCTGC TTGAGCTGCT GGACACACGG
    GGTCAGGTGC TGGCCGCGGC GTACGAGCTG CAGCGCTTCC TGCACGGGGC ACGCCAAGCC
    CTGGCGCGGG TGCAGCACAA GCAGCAGCAG CTTCCGGACG GGACTGGCCG CGACCTCAAC
    GCTGCCGAGG CCCTGCAGCG CCGACACTGT GCCTACGAGC ATGACATTCA GGCCCTCAGC
    CCCCAGGTCC AGCAGGTGCA GGACGACGGC CACCGGCTCC AGAAGGCCTA CGCTGGAGAC
    AAGGCTGAGG AGATCGGCCG CCACATGCAG GCCGTGGCCG AGGCCTGGGC CCAGCTTCAG
    GGAAGCTCTG CCGCCCGCCG GCAGCTGCTG CTGGACACCA CAGACAAGTT CCGCTTCTTC
    AAGGCTGTCC GGGAACTGAT GCTCTGGATG GATGAGGTCA ACCTGCAGAT GGATGCCCAG
    GAGCGTCCCC GGGATGTGTC CTCCGCGGAT CTAGTCATCA AGAACCAGCA AGGCATCAAG
    GCAGAGATAG AGGCCCGGGC AGACCGCTTC TCCTCCTGCA TCGACATGGG GAAGGAGCTG
    CTGGCCAGGA GCCACTATGC GGCCGAGGAG ATCTCAGAGA AGCTGTCTCA GCTGCAGGCA
    CGGCGCCAGG AGACAGCTGA GAAGTGGCAG GAGAAGATGG ACTGGCTTCA GCTGGTTTTG
    GAGGTGCTTG TGTTTGGAAG AGATGCAGGG ATGGCAGAGG CCTGGCTCTG CAGCCAGGAG
    CCACTGGTGC GCAGCGCTGA GCTGGGTTGC ACGGTCGACG AAGTTGAGAG CCTCATCAAG
    CGGCACGAGG CCTTCCAGAA GTCAGCAGTG GCCTGGGAGG AGCGATTCTG TGCGCTGGAG
    AAGCTTACTG CGCTAGAGGA GCGGGAGAAG GAGCGAAAGA GAAAGAGGGA GGAGGAGGAG
    CGGCGGAAAC AGCCGCCTGC TCCCGAACCC ACAGCCAGTG TGCCTCCAGG GGACCTGGTG
    GGCGGCCAGA CAGCTTCTGA CACCACCTGG GACGGAACCC AGCCACGGCC ACCACCATCC
    ACACAAGCAC CCAGTGTTAA TGGAGTCTGC ACAGATGGAG AGCCCTCACA GCCCCTGCTG
    GGACAACAGA GACTTGAGCA CAGCAGCTTC CCCGAAGGGC CGGGACCTGG CTCAGGGGAC
    GAAGCCAATG GGCCCCGGGG AGAGAGGCAG ACCCGGACTC GGGGCCCGGC CCCATCTGCA
    ATGCCCCAGA GCAGGTCTAC CGAGTCAGCC CATGCTGCCA CCCTGCCGCC TCGAGGCCCA
    GAGCCATCTG CCCAGGAGCA GATGGAGGGG ATGCTGTGCC GCAAGCAGGA GATGGAGGCC
    TTCGGGAAGA AGGCTGCCAA CAGGTCCTGG CAGAACGTGT ACTGTGTCCT GCGGCGTGGG
    AGCCTCGGCT TTTACAAGGA TGCCAAGGCA GCCAGCGCGG GAGTGCCATA CCACGGAGAA
    GTGCCTGTCA GCCTGGCCAG GGCCCAGGGC AGCGTCGCCT TTGATTACCG AAAGCGCAAA
    CATGTCTTCA AGCTGGGCTT ACAGGATGGA AAAGAATATT TATTCCAGGC CAAGGATGAG
    GCAGAGATGA GCTCGTGGCT ACGGGTGGTG AATGCAGCCA TTGCCACAGC GTCTTCTGCC
    TCTGGAGAGC CTGAAGAGCC GGTGGTGCCC AGCACCACCC GGGGCATGAC CCGGGCCATG
    ACCATGCCCC CAGTGTCACC CGTCGGGGCT GAGGGGCCTG TTGTGCTCCG CAGCAAAGAC
    GGCAGAGAAC GAGAGCGAGA AAAACGCTTC AGCTTCTTTA AGAAGAACAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718732.1
    CDS2401..9594
    Translation

    Target ORF information:

    RefSeq Version XM_006718669.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718669.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAATGGGA ACGGAGTGAG CAGGGTGCAC GGGAACGGCC TGCATGAGGC CAGCGAGTTT 
    TACTATGAGG CTGTGGAAGG GGCCCACAAC CCCGGGGGCC TCCTGCTTTC ACCAGCTGCT
    TTCATCAACC CTGCTCAGTA TGCCAGCGTG CTGGAAGGAC GCTTCAAACA GCTGCAAGAT
    GAACGAGAAG CTGTGCAGAA GAAAACCTTC ACCAAGTGGG TAAACTCGCA CCTGGCCCGG
    GTCACGTGCC GGGTGGGGGA CCTGTACAGC GACCTCCGGG ACGGACGCAA CCTGCTGAGG
    CTCCTCGAGG TGCTCTCGGG AGAGATACTG CCAAAGCCTA CAAAGGGCCG CATGCGGATC
    CACTGCCTGG AGAACGTGGA CAAGGCACTG CAGTTCCTCA AGGAGCAGAA AGTGCACTTG
    GAAAACATGG GCTCCCATGA CATTGTGGAC GGAAACCACC GACTGACCCT TGGGCTGGTC
    TGGACCATCA TCCTTCGATT CCAGATCCAA GACATCAGTG TGGAGACAGA AGACAACAAG
    GAGAAGAAGT CAGCCAAGGA TGCCCTGCTT CTGTGGTGCC AGATGAAGAC TGCAGGTTAT
    CCCAACGTCA ATGTACACAA CTTCACCACC AGCTGGAGAG ATGGACTAGC TTTCAACGCC
    ATCGTGCATA AACACCGGCC AGACCTGCTG GATTTTGAGT CTCTGAAGAA GTGTAATGCA
    CACTATAATC TGCAGAATGC ATTCAATCTG GCTGAAAAGG AACTGGGACT TACCAAGCTG
    CTGGATCCCG AAGACGTGAA TGTGGACCAG CCAGATGAGA AGTCAATCAT TACCTATGTG
    GCTACTTACT ACCATTACTT CTCCAAGATG AAGGCCCTGG CCGTGGAAGG CAAGAGAATT
    GGCAAGGTGC TGGACCATGC CATGGAGGCA GAGCGCCTGG TGGAGAAATA CGAGTCCCTG
    GCCTCGGAGC TGCTGCAGTG GATCGAGCAA ACGATCGTGA CCCTCAATGA CCGGCAGTTG
    GCCAACTCCC TTAGCGGGGT CCAGAACCAG CTGCAGTCCT TCAACTCCTA CCGCACCGTG
    GAGAAGCCGC CCAAGTTTAC CGAGAAAGGG AACTTGGAAG TGCTGCTCTT CACCATCCAG
    AGCAAGCTTC GGGCCAACAA CCAGAAGGTC TACACGCCCC GCGAGGGCCG GCTCATCTCG
    GACATCAACA AGGCTTGGGA GCGGCTGGAG AAGGCGGAGC ACGAGCGTGA GCTGGCCCTG
    CGCACCGAGC TCATCCGCCA GGAGAAGCTG GAGCAGCTGG CCGCCCGCTT CGACCGCAAG
    GCTGCCATGC GGGAGACCTG GCTCAGCGAG AACCAGCGCC TCGTGTCCCA GGACAACTTT
    GGGCTGGAGC TGGCAGCTGT CGAGGCAGCA GTACGGAAGC ACGAAGCCAT TGAGACGGAC
    ATCGTGGCCT ACAGCGGCCG GGTGCAGGCA GTGGACGCCG TGGCTGCAGA GCTGGCCGCC
    GAGCGCTACC ACGACATCAA GCGCATCGCC GCTCGGCAGC ACAACGTGGC ACGGCTCTGG
    GACTTCTTGC GGCAGATGGT GGCCGCCCGG CGGGAGCGGC TCCTCCTCAA CCTGGAGCTG
    CAGAAGGTGT TCCAGGACCT GCTCTACCTC ATGGACTGGA TGGAAGAGAT GAAGGGCCGG
    CTGCAGTCTC AGGACCTGGG CAGGCACCTA GCAGGAGTGG AGGACCTGCT GCAGCTGCAC
    GAGCTGGTGG AGGCAGACAT CGCCGTGCAG GCCGAGAGGG TGCGGGCCGT CAGCGCCTCT
    GCCCTGCGCT TCTGCAACCC AGGGAAAGAG TATAGACCTT GCGACCCGCA GCTGGTGTCG
    GAGCGGGTGG CCAAGCTAGA GCAGAGCTAT GAGGCACTGT GCGAGTTGGC AGCGGCGCGG
    CGGGCCCGGC TGGAGGAATC ACGGCGGCTC TGGCGTTTCC TCTGGGAGGT GGGTGAAGCT
    GAGGCCTGGG TGCGGGAGCA GCAGCACCTC CTGGCCTCAG CCGACACGGG CCGAGACCTG
    ACCGGTGCCC TCCGCCTGCT CAACAAGCAC ACAGCCCTGC GGGGCGAGAT GAGCGGCCGG
    CTGGGGCCCC TGAAGCTCAC CCTGGAGCAG GGCCAGCAGT TGGTGGCCGA GGGTCACCCT
    GGGGCAAGCC AGGCCTCTGC CCGTGCAGCT GAACTCCAAG CCCAGTGGGA GCGGCTAGAG
    GCCCTGGCCG AGGAGCGTGC CCAGCGGCTG GCCCAAGCCG CCAGCCTCTA CCAGTTCCAG
    GCCGATGCAA ACGACATGGA GGCCTGGTTG GTTGACGCAC TGCGCCTGGT GTCCAGCCCC
    GAGCTGGGGC ACGACGAGTT CTCCACGCAG GCTCTAGCCA GGCAGCATCG GGCCCTGGAG
    GAGGAGATTC GAAGCCACCG GCCAACCCTG GACGCCTTGA GGGAACAGGC AGCAGCCCTG
    CCCCCCACAC TGAGCCGCAC GCCCGAGGTG CAGAGCCGGG TGCCCACCCT GGAGCGGCAC
    TACGAGGAGC TGCAGGCCCG GGCAGGCGAG CGAGCGCGGG CCTTGGAGGC AGCCCTGGCG
    CTCTACACCA TGCTCAGCGA GGCCGGGGCC TGTGGACTCT GGGTGGAGGA GAAGGAGCAG
    TGGCTCAACG GGCTGGCCCT GCCTGAACGC CTGGAGGACC TGGAGGTCGT GCAGCAGAGG
    TTCGAGACCC TGGAGCCTGA AATGAACACC CTTGCAGCAC AAATCACCGC GGTGAATGAC
    ATTGCCGAGC AGTTACTGAA GGCCAACCCC CCAGGCAAAG ACCGCATTGT CAACACCCAG
    GAGCAGCTCA ACCACAGGTG GCAGCAGTTT CGGCGTCTGG CAGACGGCAA GAAGGCAGCT
    CTCACCTCAG CCCTGAGCAT CCAGAACTAC CACTTAGAGT GCACGGAGAC CCAGGCCTGG
    ATGAGAGAGA AGACCAAAGT CATCGAGTCC ACCCAGGGCC TAGGCAACGA TCTGGCTGGG
    GTGCTGGCCC TGCAGCGCAA GCTGGCCGGC ACGGAGCGGG ACCTGGAGGC CATCGCCGCC
    CGGGTGGGCG AACTGACTCG AGAGGCAAAT GCCCTGGCTG CCGGCCATCC CGCTCAGGCA
    GTGGCCATCA ACGCCCGGCT GAGAGAGGTG CAGACCGGCT GGGAGGACCT CAGGGCCACC
    ATGCGGCGTC GAGAAGAGTC GCTGGGGGAG GCGCGGCGGC TGCAGGACTT CTTGCGCAGC
    TTGGATGACT TCCAGGCCTG GCTAGGCCGC ACTCAGACTG CTGTGGCCTC TGAAGAAGGG
    CCGGCCACCC TGCCTGAGGC AGAGGCCCTC CTGGCCCAAC ATGCAGCCCT GCGGGGAGAG
    GTGGAGCGGG CCCAGAGCGA GTATAGCCGG CTGCGAGCCC TGGGCGAGGA GGTGACCCGG
    GACCAGGCTG ACCCCCAGTG CCTCTTCCTA CGACAGCGAC TGGAGGCCCT GGGAACTGGC
    TGGGAGGAGC TGGGCCGAAT GTGGGAGAGC CGGCAAGGTC GCCTGGCCCA GGCCCACGGC
    TTCCAGGGAT TCCTGCGGGA TGCTCGTCAG GCTGAGGGCG TGCTCAGCAG CCAGGAATAT
    GTTCTGTCTC ACACGGAGAT GCCAGGGACA CTCCAGGCTG CTGATGCTGC CATTAAAAAA
    CTGGAGGACT TCATGAGCAC CATGGACGCC AATGGGGAAC GGATCCACGG GCTCCTGGAG
    GCTGGCCGCC AGCTGGTATC TGAAGGCAAC ATCCACGCCG ACAAGATTCG GGAAAAGGCA
    GACTCCATTG AGAGGAGGCA CAAGAAGAAT CAAGACGCAG CGCAGCAATT TCTGGGCCGT
    CTTCGGGACA ACCGGGAGCA GCAGCATTTC CTGCAAGATT GTCACGAGCT GAAGCTCTGG
    ATCGACGAGA AGATGCTGAC AGCCCAGGAC GTGTCCTATG ACGAGGCCCG CAACCTGCAT
    ACTAAGTGGC AGAAGCACCA GGCATTCATG GCCGAGCTGG CTGCCAACAA AGACTGGCTG
    GACAAGGTGG ACAAGGAAGG GCGAGAGCTC ACCCTTGAGA AGCCAGAGCT GAAAGCCCTG
    GTGTCGGAGA AGCTGAGAGA CCTGCACAGG CGCTGGGACG AGCTGGAGAC CACCACCCAA
    GCCAAGGCCC GCAGCCTCTT TGATGCCAAC CGAGCTGAGC TGTTTGCCCA GAGCTGCTGT
    GCCCTGGAGA GCTGGCTGGA GAGCCTGCAG GCCCAGCTGC ACTCGGATGA CTACGGCAAG
    GACCTCACCA GCGTCAACAT CCTGCTCAAG AAGCAGCAGA TGCTGGAATG GGAGATGGCT
    GTGAGAGAGA AGGAGGTGGA GGCAATCCAG GCCCAGGCCA AAGCACTGGC CCAGGAGGAC
    CAGGGTGCAG GGGAGGTGGA GAGAACCTCG AGGGCCGTGG AGGAGAAGTT CAGGGCCTTG
    TGCCAGCCCA TGCGGGAACG CTGCCGGCGC CTGCAGGCTT CTCGCGAGCA GCACCAGTTC
    CACCGCGATG TGGAAGATGA GATTTTGTGG GTGACAGAGC GGCTGCCCAT GGCCAGCTCC
    ATGGAGCATG GCAAGGACCT GCCCAGCGTC CAGCTTCTCA TGAAGAAAAA CCAGACCCTG
    CAGAAAGAGA TTCAGGGCCA TGAGCCCCGG ATCGCGGACC TGAGGGAGCG GCAGCGTGCT
    CTAGGTGCAG CAGCAGCAGG TCCAGAGCTG GCTGAGCTGC AGGAAATGTG GAAACGCCTG
    GGCCACGAGC TGGAACTTCG AGGGAAGCGA CTGGAGGATG CCCTGCGAGC CCAGCAGTTC
    TACCGCGATG CCGCCGAGGC GGAGGCCTGG ATGGGCGAGC AGGAATTACA CATGATGGGC
    CAGGAGAAGG CCAAGGATGA GCTGAGTGCC CAGGCAGAGG TGAAGAAGCA CCAGGTGCTG
    GAGCAAGCCC TGGCCGACTA CGCGCAGACC ATCCACCAGC TGGCGGCCAG CAGCCAGGAC
    ATGATTGACC ACGAGCACCC AGAGAGCACT CGGATATCCA TCCGCCAAGC CCAGGTGGAC
    AAGCTGTATG CCGGCCTGAA GGAGCTGGCT GGAGAGCGGC GGGAGCGCCT GCAGGAGCAC
    CTCCGGCTGT GCCAGCTCCG CCGCGAGCTG GATGACCTGG AACAGTGGAT CCAGGAGCGC
    GAGGTGGTGG CGGCCTCCCA CGAGCTGGGC CAGGACTACG AGCATGTGAC TATGCTCCGA
    GACAAATTCC GAGAGTTCTC CCGGGACACA AGCACCATCG GTCAGGAGCG CGTAGATAGC
    GCCAATGCGC TGGCCAATGG GCTCATTGCT GGGGGCCATG CTGCACGGGC CACCGTGGCC
    GAGTGGAAGG ACAGTCTCAA CGAGGCCTGG GCTGACCTGC TTGAGCTGCT GGACACACGG
    GGTCAGGTGC TGGCCGCGGC GTACGAGCTG CAGCGCTTCC TGCACGGGGC ACGCCAAGCC
    CTGGCGCGGG TGCAGCACAA GCAGCAGCAG CTTCCGGACG GGACTGGCCG CGACCTCAAC
    GCTGCCGAGG CCCTGCAGCG CCGACACTGT GCCTACGAGC ATGACATTCA GGCCCTCAGC
    CCCCAGGTCC AGCAGGTGCA GGACGACGGC CACCGGCTCC AGAAGGCCTA CGCTGGAGAC
    AAGGCTGAGG AGATCGGCCG CCACATGCAG GCCGTGGCCG AGGCCTGGGC CCAGCTTCAG
    GGAAGCTCTG CCGCCCGCCG GCAGCTGCTG CTGGACACCA CAGACAAGTT CCGCTTCTTC
    AAGGCTGTCC GGGAACTGAT GCTCTGGATG GATGAGGTCA ACCTGCAGAT GGATGCCCAG
    GAGCGTCCCC GGGATGTGTC CTCCGCGGAT CTAGTCATCA AGAACCAGCA AGGCATCAAG
    GCAGAGATAG AGGCCCGGGC AGACCGCTTC TCCTCCTGCA TCGACATGGG GAAGGAGCTG
    CTGGCCAGGA GCCACTATGC GGCCGAGGAG ATCTCAGAGA AGCTGTCTCA GCTGCAGGCA
    CGGCGCCAGG AGACAGCTGA GAAGTGGCAG GAGAAGATGG ACTGGCTTCA GCTGGTTTTG
    GAGGTGCTTG TGTTTGGAAG AGATGCAGGG ATGGCAGAGG CCTGGCTCTG CAGCCAGGAG
    CCACTGGTGC GCAGCGCTGA GCTGGGTTGC ACGGTCGACG AAGTTGAGAG CCTCATCAAG
    CGGCACGAGG CCTTCCAGAA GTCAGCAGTG GCCTGGGAGG AGCGATTCTG TGCGCTGGAG
    AAGCTTACTG CGCTAGAGGA GCGGGAGAAG GAGCGAAAGA GAAAGAGGGA GGAGGAGGAG
    CGGCGGAAAC AGCCGCCTGC TCCCGAACCC ACAGCCAGTG TGCCTCCAGG GGACCTGGTG
    GGCGGCCAGA CAGCTTCTGA CACCACCTGG GACGGAACCC AGCCACGGCC ACCACCATCC
    ACACAAGCAC CCAGTGTTAA TGGAGTCTGC ACAGATGGAG AGCCCTCACA GCCCCTGCTG
    GGACAACAGA GACTTGAGCA CAGCAGCTTC CCCGAAGGGC CGGGACCTGG CTCAGGGGAC
    GAAGCCAATG GGCCCCGGGG AGAGAGGCAG ACCCGGACTC GGGGCCCGGC CCCATCTGCA
    ATGCCCCAGA GCAGGTCTAC CGAGTCAGCC CATGCTGCCA CCCTGCCGCC TCGAGGCCCA
    GAGCCATCTG CCCAGGAGCA GATGGAGGGG ATGCTGTGCC GCAAGCAGGA GATGGAGGCC
    TTCGGGAAGA AGGCTGCCAA CAGGTCCTGG CAGAACGTGT ACTGTGTCCT GCGGCGTGGG
    AGCCTCGGCT TTTACAAGGA TGCCAAGGCA GCCAGCGCGG GAGTGCCATA CCACGGAGAA
    GTGCCTGTCA GCCTGGCCAG GGCCCAGGGC AGCGTCGCCT TTGATTACCG AAAGCGCAAA
    CATGTCTTCA AGCTGGGCTT ACAGGATGGA AAAGAATATT TATTCCAGGC CAAGGATGAG
    GCAGAGATGA GCTCGTGGCT ACGGGTGGTG AATGCAGCCA TTGCCACAGC GTCTTCTGCC
    TCTGGAGAGC CTGAAGAGCC GGTGGTGCCC AGCACCACCC GGGGCATGAC CCGGGCCATG
    ACCATGCCCC CAGTGTCACC CGTCGGGGCT GAGGGGCCTG TTGTGCTCCG CAGCAAAGAC
    GGCAGAGAAC GAGAGCGAGA AAAACGCTTC AGCTTCTTTA AGAAGAACAA GTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45471
    Clone ID Related Accession (Same CDS sequence) XM_017018176.1 , XM_005274193.3 , XM_017018175.1 , XM_017018177.1 , XM_017018174.1 , XM_017018175.2 , XM_006718671.4 , XM_017018177.2 , XM_006718671.3 , XM_005274192.4 , XM_017018178.1 , NM_006946.4
    Accession Version XM_017018176.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873665.1
    CDS176..7348
    Translation

    Target ORF information:

    RefSeq Version XM_017018176.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018176.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45471
    Clone ID Related Accession (Same CDS sequence) XM_017018176.1 , XM_005274193.3 , XM_017018175.1 , XM_017018177.1 , XM_017018174.1 , XM_017018175.2 , XM_006718671.4 , XM_017018177.2 , XM_006718671.3 , XM_005274192.4 , XM_017018178.1 , NM_006946.4
    Accession Version XM_005274193.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005274193.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005274250.1
    CDS509..7681
    Translation

    Target ORF information:

    RefSeq Version XM_005274193.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005274193.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45471
    Clone ID Related Accession (Same CDS sequence) XM_017018176.1 , XM_005274193.3 , XM_017018175.1 , XM_017018177.1 , XM_017018174.1 , XM_017018175.2 , XM_006718671.4 , XM_017018177.2 , XM_006718671.3 , XM_005274192.4 , XM_017018178.1 , NM_006946.4
    Accession Version XM_017018175.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873664.1
    CDS456..7628
    Translation

    Target ORF information:

    RefSeq Version XM_017018175.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018175.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45471
    Clone ID Related Accession (Same CDS sequence) XM_017018176.1 , XM_005274193.3 , XM_017018175.1 , XM_017018177.1 , XM_017018174.1 , XM_017018175.2 , XM_006718671.4 , XM_017018177.2 , XM_006718671.3 , XM_005274192.4 , XM_017018178.1 , NM_006946.4
    Accession Version XM_017018177.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873666.1
    CDS365..7537
    Translation

    Target ORF information:

    RefSeq Version XM_017018177.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018177.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45471
    Clone ID Related Accession (Same CDS sequence) XM_017018176.1 , XM_005274193.3 , XM_017018175.1 , XM_017018177.1 , XM_017018174.1 , XM_017018175.2 , XM_006718671.4 , XM_017018177.2 , XM_006718671.3 , XM_005274192.4 , XM_017018178.1 , NM_006946.4
    Accession Version XM_017018174.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873663.1
    CDS827..7999
    Translation

    Target ORF information:

    RefSeq Version XM_017018174.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018174.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45471
    Clone ID Related Accession (Same CDS sequence) XM_017018176.1 , XM_005274193.3 , XM_017018175.1 , XM_017018177.1 , XM_017018174.1 , XM_017018175.2 , XM_006718671.4 , XM_017018177.2 , XM_006718671.3 , XM_005274192.4 , XM_017018178.1 , NM_006946.4
    Accession Version XM_017018175.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018175.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873664.1
    CDS457..7629
    Translation

    Target ORF information:

    RefSeq Version XM_017018175.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018175.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45471
    Clone ID Related Accession (Same CDS sequence) XM_017018176.1 , XM_005274193.3 , XM_017018175.1 , XM_017018177.1 , XM_017018174.1 , XM_017018175.2 , XM_006718671.4 , XM_017018177.2 , XM_006718671.3 , XM_005274192.4 , XM_017018178.1 , NM_006946.4
    Accession Version XM_006718671.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006718671.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718734.1
    CDS136..7308
    Translation

    Target ORF information:

    RefSeq Version XM_006718671.4
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718671.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45471
    Clone ID Related Accession (Same CDS sequence) XM_017018176.1 , XM_005274193.3 , XM_017018175.1 , XM_017018177.1 , XM_017018174.1 , XM_017018175.2 , XM_006718671.4 , XM_017018177.2 , XM_006718671.3 , XM_005274192.4 , XM_017018178.1 , NM_006946.4
    Accession Version XM_017018177.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017018177.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
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    3421
    3481
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    3601
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    3721
    3781
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    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873666.1
    CDS366..7538
    Translation

    Target ORF information:

    RefSeq Version XM_017018177.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018177.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45471
    Clone ID Related Accession (Same CDS sequence) XM_017018176.1 , XM_005274193.3 , XM_017018175.1 , XM_017018177.1 , XM_017018174.1 , XM_017018175.2 , XM_006718671.4 , XM_017018177.2 , XM_006718671.3 , XM_005274192.4 , XM_017018178.1 , NM_006946.4
    Accession Version XM_006718671.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006718671.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006718734.1
    CDS135..7307
    Translation

    Target ORF information:

    RefSeq Version XM_006718671.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006718671.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45471
    Clone ID Related Accession (Same CDS sequence) XM_017018176.1 , XM_005274193.3 , XM_017018175.1 , XM_017018177.1 , XM_017018174.1 , XM_017018175.2 , XM_006718671.4 , XM_017018177.2 , XM_006718671.3 , XM_005274192.4 , XM_017018178.1 , NM_006946.4
    Accession Version XM_005274192.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-12
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005274192.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005274249.1
    CDS735..7907
    Translation

    Target ORF information:

    RefSeq Version XM_005274192.4
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005274192.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu45471
    Clone ID Related Accession (Same CDS sequence) XM_017018176.1 , XM_005274193.3 , XM_017018175.1 , XM_017018177.1 , XM_017018174.1 , XM_017018175.2 , XM_006718671.4 , XM_017018177.2 , XM_006718671.3 , XM_005274192.4 , XM_017018178.1 , NM_006946.4
    Accession Version XM_017018178.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7173bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016873667.1
    CDS48..7220
    Translation

    Target ORF information:

    RefSeq Version XM_017018178.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 2 (SPTBN2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017018178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    ATGAGCAGCA CGCTGTCACC CACAGACTTT GACAGCTTGG AAATCCAGGG CCAGTACAGT 
    GACATCAACA ACCGCTGGGA CCTTCCTGAC TCGGACTGGG ACAATGACAG CAGCTCGGCC
    CGCCTCTTTG AGAGGTCTCG CATTAAGGCT CTGGCAGATG AACGAGAAGC TGTGCAGAAG
    AAAACCTTCA CCAAGTGGGT AAACTCGCAC CTGGCCCGGG TCACGTGCCG GGTGGGGGAC
    CTGTACAGCG ACCTCCGGGA CGGACGCAAC CTGCTGAGGC TCCTCGAGGT GCTCTCGGGA
    GAGATACTGC CAAAGCCTAC AAAGGGCCGC ATGCGGATCC ACTGCCTGGA GAACGTGGAC
    AAGGCACTGC AGTTCCTCAA GGAGCAGAAA GTGCACTTGG AAAACATGGG CTCCCATGAC
    ATTGTGGACG GAAACCACCG ACTGACCCTT GGGCTGGTCT GGACCATCAT CCTTCGATTC
    CAGATCCAAG ACATCAGTGT GGAGACAGAA GACAACAAGG AGAAGAAGTC AGCCAAGGAT
    GCCCTGCTTC TGTGGTGCCA GATGAAGACT GCAGGTTATC CCAACGTCAA TGTACACAAC
    TTCACCACCA GCTGGAGAGA TGGACTAGCT TTCAACGCCA TCGTGCATAA ACACCGGCCA
    GACCTGCTGG ATTTTGAGTC TCTGAAGAAG TGTAATGCAC ACTATAATCT GCAGAATGCA
    TTCAATCTGG CTGAAAAGGA ACTGGGACTT ACCAAGCTGC TGGATCCCGA AGACGTGAAT
    GTGGACCAGC CAGATGAGAA GTCAATCATT ACCTATGTGG CTACTTACTA CCATTACTTC
    TCCAAGATGA AGGCCCTGGC CGTGGAAGGC AAGAGAATTG GCAAGGTGCT GGACCATGCC
    ATGGAGGCAG AGCGCCTGGT GGAGAAATAC GAGTCCCTGG CCTCGGAGCT GCTGCAGTGG
    ATCGAGCAAA CGATCGTGAC CCTCAATGAC CGGCAGTTGG CCAACTCCCT TAGCGGGGTC
    CAGAACCAGC TGCAGTCCTT CAACTCCTAC CGCACCGTGG AGAAGCCGCC CAAGTTTACC
    GAGAAAGGGA ACTTGGAAGT GCTGCTCTTC ACCATCCAGA GCAAGCTTCG GGCCAACAAC
    CAGAAGGTCT ACACGCCCCG CGAGGGCCGG CTCATCTCGG ACATCAACAA GGCTTGGGAG
    CGGCTGGAGA AGGCGGAGCA CGAGCGTGAG CTGGCCCTGC GCACCGAGCT CATCCGCCAG
    GAGAAGCTGG AGCAGCTGGC CGCCCGCTTC GACCGCAAGG CTGCCATGCG GGAGACCTGG
    CTCAGCGAGA ACCAGCGCCT CGTGTCCCAG GACAACTTTG GGCTGGAGCT GGCAGCTGTC
    GAGGCAGCAG TACGGAAGCA CGAAGCCATT GAGACGGACA TCGTGGCCTA CAGCGGCCGG
    GTGCAGGCAG TGGACGCCGT GGCTGCAGAG CTGGCCGCCG AGCGCTACCA CGACATCAAG
    CGCATCGCCG CTCGGCAGCA CAACGTGGCA CGGCTCTGGG ACTTCTTGCG GCAGATGGTG
    GCCGCCCGGC GGGAGCGGCT CCTCCTCAAC CTGGAGCTGC AGAAGGTGTT CCAGGACCTG
    CTCTACCTCA TGGACTGGAT GGAAGAGATG AAGGGCCGGC TGCAGTCTCA GGACCTGGGC
    AGGCACCTAG CAGGAGTGGA GGACCTGCTG CAGCTGCACG AGCTGGTGGA GGCAGACATC
    GCCGTGCAGG CCGAGAGGGT GCGGGCCGTC AGCGCCTCTG CCCTGCGCTT CTGCAACCCA
    GGGAAAGAGT ATAGACCTTG CGACCCGCAG CTGGTGTCGG AGCGGGTGGC CAAGCTAGAG
    CAGAGCTATG AGGCACTGTG CGAGTTGGCA GCGGCGCGGC GGGCCCGGCT GGAGGAATCA
    CGGCGGCTCT GGCGTTTCCT CTGGGAGGTG GGTGAAGCTG AGGCCTGGGT GCGGGAGCAG
    CAGCACCTCC TGGCCTCAGC CGACACGGGC CGAGACCTGA CCGGTGCCCT CCGCCTGCTC
    AACAAGCACA CAGCCCTGCG GGGCGAGATG AGCGGCCGGC TGGGGCCCCT GAAGCTCACC
    CTGGAGCAGG GCCAGCAGTT GGTGGCCGAG GGTCACCCTG GGGCAAGCCA GGCCTCTGCC
    CGTGCAGCTG AACTCCAAGC CCAGTGGGAG CGGCTAGAGG CCCTGGCCGA GGAGCGTGCC
    CAGCGGCTGG CCCAAGCCGC CAGCCTCTAC CAGTTCCAGG CCGATGCAAA CGACATGGAG
    GCCTGGTTGG TTGACGCACT GCGCCTGGTG TCCAGCCCCG AGCTGGGGCA CGACGAGTTC
    TCCACGCAGG CTCTAGCCAG GCAGCATCGG GCCCTGGAGG AGGAGATTCG AAGCCACCGG
    CCAACCCTGG ACGCCTTGAG GGAACAGGCA GCAGCCCTGC CCCCCACACT GAGCCGCACG
    CCCGAGGTGC AGAGCCGGGT GCCCACCCTG GAGCGGCACT ACGAGGAGCT GCAGGCCCGG
    GCAGGCGAGC GAGCGCGGGC CTTGGAGGCA GCCCTGGCGC TCTACACCAT GCTCAGCGAG
    GCCGGGGCCT GTGGACTCTG GGTGGAGGAG AAGGAGCAGT GGCTCAACGG GCTGGCCCTG
    CCTGAACGCC TGGAGGACCT GGAGGTCGTG CAGCAGAGGT TCGAGACCCT GGAGCCTGAA
    ATGAACACCC TTGCAGCACA AATCACCGCG GTGAATGACA TTGCCGAGCA GTTACTGAAG
    GCCAACCCCC CAGGCAAAGA CCGCATTGTC AACACCCAGG AGCAGCTCAA CCACAGGTGG
    CAGCAGTTTC GGCGTCTGGC AGACGGCAAG AAGGCAGCTC TCACCTCAGC CCTGAGCATC
    CAGAACTACC ACTTAGAGTG CACGGAGACC CAGGCCTGGA TGAGAGAGAA GACCAAAGTC
    ATCGAGTCCA CCCAGGGCCT AGGCAACGAT CTGGCTGGGG TGCTGGCCCT GCAGCGCAAG
    CTGGCCGGCA CGGAGCGGGA CCTGGAGGCC ATCGCCGCCC GGGTGGGCGA ACTGACTCGA
    GAGGCAAATG CCCTGGCTGC CGGCCATCCC GCTCAGGCAG TGGCCATCAA CGCCCGGCTG
    AGAGAGGTGC AGACCGGCTG GGAGGACCTC AGGGCCACCA TGCGGCGTCG AGAAGAGTCG
    CTGGGGGAGG CGCGGCGGCT GCAGGACTTC TTGCGCAGCT TGGATGACTT CCAGGCCTGG
    CTAGGCCGCA CTCAGACTGC TGTGGCCTCT GAAGAAGGGC CGGCCACCCT GCCTGAGGCA
    GAGGCCCTCC TGGCCCAACA TGCAGCCCTG CGGGGAGAGG TGGAGCGGGC CCAGAGCGAG
    TATAGCCGGC TGCGAGCCCT GGGCGAGGAG GTGACCCGGG ACCAGGCTGA CCCCCAGTGC
    CTCTTCCTAC GACAGCGACT GGAGGCCCTG GGAACTGGCT GGGAGGAGCT GGGCCGAATG
    TGGGAGAGCC GGCAAGGTCG CCTGGCCCAG GCCCACGGCT TCCAGGGATT CCTGCGGGAT
    GCTCGTCAGG CTGAGGGCGT GCTCAGCAGC CAGGAATATG TTCTGTCTCA CACGGAGATG
    CCAGGGACAC TCCAGGCTGC TGATGCTGCC ATTAAAAAAC TGGAGGACTT CATGAGCACC
    ATGGACGCCA ATGGGGAACG GATCCACGGG CTCCTGGAGG CTGGCCGCCA GCTGGTATCT
    GAAGGCAACA TCCACGCCGA CAAGATTCGG GAAAAGGCAG ACTCCATTGA GAGGAGGCAC
    AAGAAGAATC AAGACGCAGC GCAGCAATTT CTGGGCCGTC TTCGGGACAA CCGGGAGCAG
    CAGCATTTCC TGCAAGATTG TCACGAGCTG AAGCTCTGGA TCGACGAGAA GATGCTGACA
    GCCCAGGACG TGTCCTATGA CGAGGCCCGC AACCTGCATA CTAAGTGGCA GAAGCACCAG
    GCATTCATGG CCGAGCTGGC TGCCAACAAA GACTGGCTGG ACAAGGTGGA CAAGGAAGGG
    CGAGAGCTCA CCCTTGAGAA GCCAGAGCTG AAAGCCCTGG TGTCGGAGAA GCTGAGAGAC
    CTGCACAGGC GCTGGGACGA GCTGGAGACC ACCACCCAAG CCAAGGCCCG CAGCCTCTTT
    GATGCCAACC GAGCTGAGCT GTTTGCCCAG AGCTGCTGTG CCCTGGAGAG CTGGCTGGAG
    AGCCTGCAGG CCCAGCTGCA CTCGGATGAC TACGGCAAGG ACCTCACCAG CGTCAACATC
    CTGCTCAAGA AGCAGCAGAT GCTGGAATGG GAGATGGCTG TGAGAGAGAA GGAGGTGGAG
    GCAATCCAGG CCCAGGCCAA AGCACTGGCC CAGGAGGACC AGGGTGCAGG GGAGGTGGAG
    AGAACCTCGA GGGCCGTGGA GGAGAAGTTC AGGGCCTTGT GCCAGCCCAT GCGGGAACGC
    TGCCGGCGCC TGCAGGCTTC TCGCGAGCAG CACCAGTTCC ACCGCGATGT GGAAGATGAG
    ATTTTGTGGG TGACAGAGCG GCTGCCCATG GCCAGCTCCA TGGAGCATGG CAAGGACCTG
    CCCAGCGTCC AGCTTCTCAT GAAGAAAAAC CAGACCCTGC AGAAAGAGAT TCAGGGCCAT
    GAGCCCCGGA TCGCGGACCT GAGGGAGCGG CAGCGTGCTC TAGGTGCAGC AGCAGCAGGT
    CCAGAGCTGG CTGAGCTGCA GGAAATGTGG AAACGCCTGG GCCACGAGCT GGAACTTCGA
    GGGAAGCGAC TGGAGGATGC CCTGCGAGCC CAGCAGTTCT ACCGCGATGC CGCCGAGGCG
    GAGGCCTGGA TGGGCGAGCA GGAATTACAC ATGATGGGCC AGGAGAAGGC CAAGGATGAG
    CTGAGTGCCC AGGCAGAGGT GAAGAAGCAC CAGGTGCTGG AGCAAGCCCT GGCCGACTAC
    GCGCAGACCA TCCACCAGCT GGCGGCCAGC AGCCAGGACA TGATTGACCA CGAGCACCCA
    GAGAGCACTC GGATATCCAT CCGCCAAGCC CAGGTGGACA AGCTGTATGC CGGCCTGAAG
    GAGCTGGCTG GAGAGCGGCG GGAGCGCCTG CAGGAGCACC TCCGGCTGTG CCAGCTCCGC
    CGCGAGCTGG ATGACCTGGA ACAGTGGATC CAGGAGCGCG AGGTGGTGGC GGCCTCCCAC
    GAGCTGGGCC AGGACTACGA GCATGTGACT ATGCTCCGAG ACAAATTCCG AGAGTTCTCC
    CGGGACACAA GCACCATCGG TCAGGAGCGC GTAGATAGCG CCAATGCGCT GGCCAATGGG
    CTCATTGCTG GGGGCCATGC TGCACGGGCC ACCGTGGCCG AGTGGAAGGA CAGTCTCAAC
    GAGGCCTGGG CTGACCTGCT TGAGCTGCTG GACACACGGG GTCAGGTGCT GGCCGCGGCG
    TACGAGCTGC AGCGCTTCCT GCACGGGGCA CGCCAAGCCC TGGCGCGGGT GCAGCACAAG
    CAGCAGCAGC TTCCGGACGG GACTGGCCGC GACCTCAACG CTGCCGAGGC CCTGCAGCGC
    CGACACTGTG CCTACGAGCA TGACATTCAG GCCCTCAGCC CCCAGGTCCA GCAGGTGCAG
    GACGACGGCC ACCGGCTCCA GAAGGCCTAC GCTGGAGACA AGGCTGAGGA GATCGGCCGC
    CACATGCAGG CCGTGGCCGA GGCCTGGGCC CAGCTTCAGG GAAGCTCTGC CGCCCGCCGG
    CAGCTGCTGC TGGACACCAC AGACAAGTTC CGCTTCTTCA AGGCTGTCCG GGAACTGATG
    CTCTGGATGG ATGAGGTCAA CCTGCAGATG GATGCCCAGG AGCGTCCCCG GGATGTGTCC
    TCCGCGGATC TAGTCATCAA GAACCAGCAA GGCATCAAGG CAGAGATAGA GGCCCGGGCA
    GACCGCTTCT CCTCCTGCAT CGACATGGGG AAGGAGCTGC TGGCCAGGAG CCACTATGCG
    GCCGAGGAGA TCTCAGAGAA GCTGTCTCAG CTGCAGGCAC GGCGCCAGGA GACAGCTGAG
    AAGTGGCAGG AGAAGATGGA CTGGCTTCAG CTGGTTTTGG AGGTGCTTGT GTTTGGAAGA
    GATGCAGGGA TGGCAGAGGC CTGGCTCTGC AGCCAGGAGC CACTGGTGCG CAGCGCTGAG
    CTGGGTTGCA CGGTCGACGA AGTTGAGAGC CTCATCAAGC GGCACGAGGC CTTCCAGAAG
    TCAGCAGTGG CCTGGGAGGA GCGATTCTGT GCGCTGGAGA AGCTTACTGC GCTAGAGGAG
    CGGGAGAAGG AGCGAAAGAG AAAGAGGGAG GAGGAGGAGC GGCGGAAACA GCCGCCTGCT
    CCCGAACCCA CAGCCAGTGT GCCTCCAGGG GACCTGGTGG GCGGCCAGAC AGCTTCTGAC
    ACCACCTGGG ACGGAACCCA GCCACGGCCA CCACCATCCA CACAAGCACC CAGTGTTAAT
    GGAGTCTGCA CAGATGGAGA GCCCTCACAG CCCCTGCTGG GACAACAGAG ACTTGAGCAC
    AGCAGCTTCC CCGAAGGGCC GGGACCTGGC TCAGGGGACG AAGCCAATGG GCCCCGGGGA
    GAGAGGCAGA CCCGGACTCG GGGCCCGGCC CCATCTGCAA TGCCCCAGAG CAGGTCTACC
    GAGTCAGCCC ATGCTGCCAC CCTGCCGCCT CGAGGCCCAG AGCCATCTGC CCAGGAGCAG
    ATGGAGGGGA TGCTGTGCCG CAAGCAGGAG ATGGAGGCCT TCGGGAAGAA GGCTGCCAAC
    AGGTCCTGGC AGAACGTGTA CTGTGTCCTG CGGCGTGGGA GCCTCGGCTT TTACAAGGAT
    GCCAAGGCAG CCAGCGCGGG AGTGCCATAC CACGGAGAAG TGCCTGTCAG CCTGGCCAGG
    GCCCAGGGCA GCGTCGCCTT TGATTACCGA AAGCGCAAAC ATGTCTTCAA GCTGGGCTTA
    CAGGATGGAA AAGAATATTT ATTCCAGGCC AAGGATGAGG CAGAGATGAG CTCGTGGCTA
    CGGGTGGTGA ATGCAGCCAT TGCCACAGCG TCTTCTGCCT CTGGAGAGCC TGAAGAGCCG
    GTGGTGCCCA GCACCACCCG GGGCATGACC CGGGCCATGA CCATGCCCCC AGTGTCACCC
    GTCGGGGCTG AGGGGCCTGT TGTGCTCCGC AGCAAAGACG GCAGAGAACG AGAGCGAGAA
    AAACGCTTCA GCTTCTTTAA GAAGAACAAG TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.