SPTA1 cDNA ORF clone, Homo sapiens(human)

The following SPTA1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTA1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu60718 XM_011509919.3
Latest version!
Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu28487 NM_003126.4
Latest version!
Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu28487 XM_011509916.2
Latest version!
Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu60716 XM_011509917.3
Latest version!
Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu60717 XM_011509918.3
Latest version!
Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
View Old Accession Versions >>
OHu60717 XM_011509918.2 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1091.30
$1559.00
OHu60718 XM_011509919.2 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu28487 NM_003126.2 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu28487 NM_003126.3 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
OHu60716 XM_011509917.2 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 30 $1301.30
$1859.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu60718
    Clone ID Related Accession (Same CDS sequence) XM_011509919.2 , XM_011509919.3
    Accession Version XM_011509919.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spectrin alpha chain, erythrocytic 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509919.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GGAGAAGTTG ATACGAGTGA GCTCCCAGGA CTATGGGAGA GATCTTCAGG GGGTTCAGAA
    CTTGCTGAAG AAGCACAAAC GCCTAGAGGG GGAGCTGGTG GCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508221.1
    CDS116..5203
    Translation

    Target ORF information:

    RefSeq Version XM_011509919.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011509919.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GGAGAAGTTG ATACGAGTGA GCTCCCAGGA CTATGGGAGA GATCTTCAGG GGGTTCAGAA
    CTTGCTGAAG AAGCACAAAC GCCTAGAGGG GGAGCTGGTG GCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60718
    Clone ID Related Accession (Same CDS sequence) XM_011509919.2 , XM_011509919.3
    Accession Version XM_011509919.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5088bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin alpha chain, erythrocytic 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509919.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GGAGAAGTTG ATACGAGTGA GCTCCCAGGA CTATGGGAGA GATCTTCAGG GGGTTCAGAA
    CTTGCTGAAG AAGCACAAAC GCCTAGAGGG GGAGCTGGTG GCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508221.1
    CDS127..5214
    Translation

    Target ORF information:

    RefSeq Version XM_011509919.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011509919.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GGAGAAGTTG ATACGAGTGA GCTCCCAGGA CTATGGGAGA GATCTTCAGG GGGTTCAGAA
    CTTGCTGAAG AAGCACAAAC GCCTAGAGGG GGAGCTGGTG GCCCATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28487
    Clone ID Related Accession (Same CDS sequence) NM_003126.4 , XM_011509916.2 , NM_003126.2 , NM_003126.3
    Accession Version NM_003126.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-10
    Organism Homo sapiens(human)
    Product spectrin alpha chain, erythrocytic 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL353894.14, M61877.1, CD616351.1 and BU665064.1. On May 17, 2019 this sequence version replaced NM_003126.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M61877.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000643759.2/ ENSP00000495214.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
    CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
    AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
    GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
    CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
    CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT
    AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
    AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
    AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
    GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
    TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
    ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
    CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003117.2
    CDS199..7458
    Translation

    Target ORF information:

    RefSeq Version NM_003126.4
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003126.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
    CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
    AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
    GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
    CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
    CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT
    AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
    AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
    AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
    GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
    TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
    ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
    CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28487
    Clone ID Related Accession (Same CDS sequence) NM_003126.4 , XM_011509916.2 , NM_003126.2 , NM_003126.3
    Accession Version NM_003126.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-04-27
    Organism Homo sapiens(Human)
    Product spectrin alpha chain, erythrocytic 1
    Comment REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL353894.14, M61877.1, CD616351.1 and BU665064.1. This sequence is a reference standard in the RefSeqGene project. On Sep 22, 2006 this sequence version replaced NM_003126.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M61877.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
    CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
    AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
    GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
    CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
    CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT
    AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
    AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
    AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
    GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
    TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
    ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
    CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003117.2
    CDS200..7459
    Translation

    Target ORF information:

    RefSeq Version NM_003126.2
    Organism Homo sapiens(Human)
    Definition Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003126.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
    CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
    AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
    GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
    CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
    CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT
    AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
    AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
    AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
    GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
    TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
    ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
    CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28487
    Clone ID Related Accession (Same CDS sequence) NM_003126.4 , XM_011509916.2 , NM_003126.2 , NM_003126.3
    Accession Version NM_003126.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product spectrin alpha chain, erythrocytic 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL353894.14, M61877.1, CD616351.1 and BU665064.1. This sequence is a reference standard in the RefSeqGene project. On Aug 24, 2017 this sequence version replaced NM_003126.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M61877.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
    CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
    AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
    GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
    CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
    CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT
    AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
    AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
    AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
    GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
    TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
    ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
    CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_003117.2
    CDS199..7458
    Translation

    Target ORF information:

    RefSeq Version NM_003126.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_003126.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
    CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
    AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
    GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
    CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
    CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT
    AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
    AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
    AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
    GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
    TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
    ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
    CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu28487
    Clone ID Related Accession (Same CDS sequence) NM_003126.4 , XM_011509916.2 , NM_003126.2 , NM_003126.3
    Accession Version XM_011509916.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin alpha chain, erythrocytic 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509916.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
    CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
    AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
    GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
    CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
    CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT
    AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
    AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
    AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
    GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
    TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
    ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
    CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508218.1
    CDS78..7337
    Translation

    Target ORF information:

    RefSeq Version XM_011509916.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011509916.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT
    CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA
    AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG
    GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG
    CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC
    CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT
    AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG
    AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG
    AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT
    GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC
    TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT
    ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT
    CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60716
    Clone ID Related Accession (Same CDS sequence) XM_011509917.3 , XM_011509917.2
    Accession Version XM_011509917.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin alpha chain, erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509917.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC
    AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC
    CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT
    CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC
    CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACATCAAA
    GGTGTGAGTG AAGAGACTCT AAAGGAATTT AGCACAATCT ATAAACACTT TGATGAGAAT
    TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC
    TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG
    GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG
    GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCAGAG
    GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA
    TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC
    TATGACTACG TTGGCTTCAC CAATTCCTAC TTTGGCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508219.1
    CDS126..7367
    Translation

    Target ORF information:

    RefSeq Version XM_011509917.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011509917.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC
    AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC
    CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT
    CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC
    CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACATCAAA
    GGTGTGAGTG AAGAGACTCT AAAGGAATTT AGCACAATCT ATAAACACTT TGATGAGAAT
    TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC
    TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG
    GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG
    GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCAGAG
    GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA
    TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC
    TATGACTACG TTGGCTTCAC CAATTCCTAC TTTGGCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60716
    Clone ID Related Accession (Same CDS sequence) XM_011509917.3 , XM_011509917.2
    Accession Version XM_011509917.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7242bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spectrin alpha chain, erythrocytic 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509917.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC
    AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC
    CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT
    CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC
    CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACATCAAA
    GGTGTGAGTG AAGAGACTCT AAAGGAATTT AGCACAATCT ATAAACACTT TGATGAGAAT
    TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC
    TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG
    GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG
    GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCAGAG
    GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA
    TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC
    TATGACTACG TTGGCTTCAC CAATTCCTAC TTTGGCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508219.1
    CDS114..7355
    Translation

    Target ORF information:

    RefSeq Version XM_011509917.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011509917.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA
    AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG
    CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA
    TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC
    TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA
    CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG
    GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC
    AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC
    CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT
    CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC
    CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACATCAAA
    GGTGTGAGTG AAGAGACTCT AAAGGAATTT AGCACAATCT ATAAACACTT TGATGAGAAT
    TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC
    TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG
    GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG
    GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCAGAG
    GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA
    TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC
    TATGACTACG TTGGCTTCAC CAATTCCTAC TTTGGCAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60717
    Clone ID Related Accession (Same CDS sequence) XM_011509918.2 , XM_011509918.3
    Accession Version XM_011509918.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6126bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spectrin alpha chain, erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508220.1
    CDS114..6239
    Translation

    Target ORF information:

    RefSeq Version XM_011509918.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011509918.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60717
    Clone ID Related Accession (Same CDS sequence) XM_011509918.2 , XM_011509918.3
    Accession Version XM_011509918.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6126bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin alpha chain, erythrocytic 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509918.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011508220.1
    CDS126..6251
    Translation

    Target ORF information:

    RefSeq Version XM_011509918.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011509918.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
    GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG
    CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA
    GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC
    TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG
    GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC
    ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG
    GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC
    CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA
    GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT
    GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT
    GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT
    GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG
    TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC
    AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA
    GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG
    AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG
    ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC
    AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT
    GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA
    GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC
    TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT
    CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC
    CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC
    TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA
    AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC
    TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC
    AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG
    TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG
    TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG
    AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT
    CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA
    CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT
    CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG
    ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG
    CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC
    GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG
    GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT
    TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG
    CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA
    GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC
    CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG
    AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA
    GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT
    ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG
    TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT
    ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG
    CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT
    CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA
    AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA
    GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT
    GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG
    ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT
    GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA
    CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG
    AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT
    GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA
    GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA
    TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC
    AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG
    AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT
    CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC
    ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG
    CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT
    CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT
    CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC
    TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA
    GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC
    CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG
    GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC
    CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA
    GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC
    AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC
    ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC
    AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC
    AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG
    CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA
    CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC
    CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG
    CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG
    AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG
    TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT
    TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG
    GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC
    GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA
    TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG
    GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC
    TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG
    GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA
    GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC
    TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA
    TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT
    TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG
    CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA
    CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT
    TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG
    AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA
    GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT
    GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC
    CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC
    AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG
    CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG
    GTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.