SPTA1 gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
XM_011509918.2	XP_011508220.1	spectrin alpha chain, erythrocytic 1 isoform X3
XM_011509919.2	XP_011508221.1	spectrin alpha chain, erythrocytic 1 isoform X4
XM_011509917.3	XP_011508219.1	spectrin alpha chain, erythrocytic 1 isoform X2
XM_011509918.3	XP_011508220.1	spectrin alpha chain, erythrocytic 1 isoform X3
NM_003126.4	NP_003117.2	spectrin alpha chain, erythrocytic 1
XM_011509916.2	XP_011508218.1	spectrin alpha chain, erythrocytic 1 isoform X1
XM_011509919.3	XP_011508221.1	spectrin alpha chain, erythrocytic 1 isoform X4
NM_003126.2	NP_003117.2	spectrin alpha chain, erythrocytic 1
NM_003126.3	NP_003117.2	spectrin alpha chain, erythrocytic 1
XM_011509917.2	XP_011508219.1	spectrin alpha chain, erythrocytic 1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu60718	XM_011509919.3 Latest version!	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu28487	NM_003126.4 Latest version!	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu28487	XM_011509916.2 Latest version!	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu60716	XM_011509917.3 Latest version!	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu60717	XM_011509918.3 Latest version!	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
View Old Accession Versions >>
OHu60717	XM_011509918.2	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1091.30 $1559.00
OHu60718	XM_011509919.2	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu28487	NM_003126.2	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu28487	NM_003126.3	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
OHu60716	XM_011509917.2	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1301.30 $1859.00
Hide Old Accession Versions >>

SPTA1 ( XM_011509919.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508221.1 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu60718
Clone ID Related Accession (Same CDS sequence)	XM_011509919.2 , XM_011509919.3
Accession Version	XM_011509919.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509919.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GGAGAAGTTG ATACGAGTGA GCTCCCAGGA CTATGGGAGA GATCTTCAGG GGGTTCAGAA 
CTTGCTGAAG AAGCACAAAC GCCTAGAGGG GGAGCTGGTG GCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508221.1
CDS	116..5203
Translation	MEQFPKETVVESSGPKVLETAEEIQERRQEVLTRYQSFKERVAERGQKLEDSYHLQVFKRDADDLGKWIMEKVNILTDKSYEDPTNIQGKYQKHQSLEAEVQTKSRLMSELEKTREERFTMGHSAHEETKAHIEELRHLWDLLLELTLEKGDQLLRALKFQQYVQECADILEWIGDKEAIATSVELGEDWERTEVLHKKFEDFQVELVAKEGRVVEVNQYANECAEENHPDLPLIQSKQNEVNAAWERLRGLALQRQKALSNAANLQRFKRDVTEAIQWIKEKEPVLTSEDYGKDLVASEGLFHSHKGLERNLAVMSDKVKELCAKAEKLTLSHPSDAPQIQEMKEDLVSSWEHIRALATSRYEKLQATYWYHRFSSDFDELSGWMNEKTAAINADELPTDVAGGEVLLDRHQQHKHEIDSYDDRFQSADETGQDLVNANHEASDEVREKMEILDNNWTALLELWDERHRQYEQCLDFHLFYRDSEQVDSWMSRQEAFLENEDLGNSLGSAEALLQKHEDFEEAFTAQEEKIITVDKTATKLIGDDHYDSENIKAIRDGLLARRDALREKAATRRRLLKESLLLQKLYEDSDDLKNWINKKKKLADDEDYKDIQNLKSRVQKQQVFEKELAVNKTQLENIQKTGQEMIEGGHYASDNVTTRLSEVASLWEELLEATKQKGTQLHEANQQLQFENNAEDLQRWLEDVEWQVTSEDYGKGLAEVQNRLRKHGLLESAVAARQDQVDILTDLAAYFEEIGHPDSKDIRARQESLVCRFEALKEPLATRKKKLLDLLHLQLICRDTEDEEAWIQETEPSATSTYLGKDLIASKKLLNRHRVILENIASHEPRIQEITERGNKMVEEGHFAAEDVASRVKSLNQNMESLRARAARRQNDLEANVQFQQYLADLHEAETWIREKEPIVDNTNYGADEEAAGALLKKHEAFLLDLNSFGDSMKALRNQANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAADHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKVADDLLFEGLLTPEGAQIRQELNSRWGSLQRLADEQRQLLGSAHAVEVFHREADDTKEQIEKKCQALSAADPGSDLFSVQALQRRHEGFERDLVPLGDKVTILGETAERLSESHPDATEDLQRQKMELNEAWEDLQGRTKDRKESLNEAQKFYLFLSKARDLQNWISSIGGMVSSQELAEDLTGIEILLERHQEHRADMEAEAPTFQALEDFSAELIDSGHHASPEIEKKLQAVKLERDDLEKAWEKRKKILDQCLELQMFQGNCDQVESWMVARENSLRSDDKSSLDSLEALMKKRDDLDKAITAQEGKITDLEHFAESLIADEHYAKEEIATRLQRVLDRWKALKAQLIDERTKLGDYANLKQFYRDLEELEEWISEMLPTACDESYKDATNIQRKYLKHQTFAHEVDGRSEQVHGVINLGNSLIECSACDGNEEAMKEQLEQLKEHWDHLLERTNDKGKKLNEASRQQRFNTSIRDFEFWLSEAETLLAMKDQARDLASAGNLLKKHQLLEREMLAREGEVDTSELPGLWERSSGGSELAEEAQTPRGGAGGP

Target ORF information:

RefSeq Version	XM_011509919.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509919.2

ORF Insert Sequence:

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GGAGAAGTTG ATACGAGTGA GCTCCCAGGA CTATGGGAGA GATCTTCAGG GGGTTCAGAA 
CTTGCTGAAG AAGCACAAAC GCCTAGAGGG GGAGCTGGTG GCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTA1 ( XM_011509919.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508221.1 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OHu60718
Clone ID Related Accession (Same CDS sequence)	XM_011509919.2 , XM_011509919.3
Accession Version	XM_011509919.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, erythrocytic 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509919.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GGAGAAGTTG ATACGAGTGA GCTCCCAGGA CTATGGGAGA GATCTTCAGG GGGTTCAGAA 
CTTGCTGAAG AAGCACAAAC GCCTAGAGGG GGAGCTGGTG GCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508221.1
CDS	127..5214
Translation	MEQFPKETVVESSGPKVLETAEEIQERRQEVLTRYQSFKERVAERGQKLEDSYHLQVFKRDADDLGKWIMEKVNILTDKSYEDPTNIQGKYQKHQSLEAEVQTKSRLMSELEKTREERFTMGHSAHEETKAHIEELRHLWDLLLELTLEKGDQLLRALKFQQYVQECADILEWIGDKEAIATSVELGEDWERTEVLHKKFEDFQVELVAKEGRVVEVNQYANECAEENHPDLPLIQSKQNEVNAAWERLRGLALQRQKALSNAANLQRFKRDVTEAIQWIKEKEPVLTSEDYGKDLVASEGLFHSHKGLERNLAVMSDKVKELCAKAEKLTLSHPSDAPQIQEMKEDLVSSWEHIRALATSRYEKLQATYWYHRFSSDFDELSGWMNEKTAAINADELPTDVAGGEVLLDRHQQHKHEIDSYDDRFQSADETGQDLVNANHEASDEVREKMEILDNNWTALLELWDERHRQYEQCLDFHLFYRDSEQVDSWMSRQEAFLENEDLGNSLGSAEALLQKHEDFEEAFTAQEEKIITVDKTATKLIGDDHYDSENIKAIRDGLLARRDALREKAATRRRLLKESLLLQKLYEDSDDLKNWINKKKKLADDEDYKDIQNLKSRVQKQQVFEKELAVNKTQLENIQKTGQEMIEGGHYASDNVTTRLSEVASLWEELLEATKQKGTQLHEANQQLQFENNAEDLQRWLEDVEWQVTSEDYGKGLAEVQNRLRKHGLLESAVAARQDQVDILTDLAAYFEEIGHPDSKDIRARQESLVCRFEALKEPLATRKKKLLDLLHLQLICRDTEDEEAWIQETEPSATSTYLGKDLIASKKLLNRHRVILENIASHEPRIQEITERGNKMVEEGHFAAEDVASRVKSLNQNMESLRARAARRQNDLEANVQFQQYLADLHEAETWIREKEPIVDNTNYGADEEAAGALLKKHEAFLLDLNSFGDSMKALRNQANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAADHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKVADDLLFEGLLTPEGAQIRQELNSRWGSLQRLADEQRQLLGSAHAVEVFHREADDTKEQIEKKCQALSAADPGSDLFSVQALQRRHEGFERDLVPLGDKVTILGETAERLSESHPDATEDLQRQKMELNEAWEDLQGRTKDRKESLNEAQKFYLFLSKARDLQNWISSIGGMVSSQELAEDLTGIEILLERHQEHRADMEAEAPTFQALEDFSAELIDSGHHASPEIEKKLQAVKLERDDLEKAWEKRKKILDQCLELQMFQGNCDQVESWMVARENSLRSDDKSSLDSLEALMKKRDDLDKAITAQEGKITDLEHFAESLIADEHYAKEEIATRLQRVLDRWKALKAQLIDERTKLGDYANLKQFYRDLEELEEWISEMLPTACDESYKDATNIQRKYLKHQTFAHEVDGRSEQVHGVINLGNSLIECSACDGNEEAMKEQLEQLKEHWDHLLERTNDKGKKLNEASRQQRFNTSIRDFEFWLSEAETLLAMKDQARDLASAGNLLKKHQLLEREMLAREGEVDTSELPGLWERSSGGSELAEEAQTPRGGAGGP

Target ORF information:

RefSeq Version	XM_011509919.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509919.3

ORF Insert Sequence:

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GGAGAAGTTG ATACGAGTGA GCTCCCAGGA CTATGGGAGA GATCTTCAGG GGGTTCAGAA 
CTTGCTGAAG AAGCACAAAC GCCTAGAGGG GGAGCTGGTG GCCCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTA1 ( NM_003126.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_003117.2 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA.

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CloneID	OHu28487
Clone ID Related Accession (Same CDS sequence)	NM_003126.4 , XM_011509916.2 , NM_003126.2 , NM_003126.3
Accession Version	NM_003126.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-10
Organism	Homo sapiens(human)
Product	spectrin alpha chain, erythrocytic 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL353894.14, M61877.1, CD616351.1 and BU665064.1. On May 17, 2019 this sequence version replaced NM_003126.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M61877.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000643759.2/ ENSP00000495214.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA 
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG 
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA 
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC 
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA 
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG 
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT 
CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA 
AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG 
GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG 
CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC 
CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT 
AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG 
AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG 
AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT 
GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC 
TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT 
ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT 
CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003117.2
CDS	199..7458
Translation	MEQFPKETVVESSGPKVLETAEEIQERRQEVLTRYQSFKERVAERGQKLEDSYHLQVFKRDADDLGKWIMEKVNILTDKSYEDPTNIQGKYQKHQSLEAEVQTKSRLMSELEKTREERFTMGHSAHEETKAHIEELRHLWDLLLELTLEKGDQLLRALKFQQYVQECADILEWIGDKEAIATSVELGEDWERTEVLHKKFEDFQVELVAKEGRVVEVNQYANECAEENHPDLPLIQSKQNEVNAAWERLRGLALQRQKALSNAANLQRFKRDVTEAIQWIKEKEPVLTSEDYGKDLVASEGLFHSHKGLERNLAVMSDKVKELCAKAEKLTLSHPSDAPQIQEMKEDLVSSWEHIRALATSRYEKLQATYWYHRFSSDFDELSGWMNEKTAAINADELPTDVAGGEVLLDRHQQHKHEIDSYDDRFQSADETGQDLVNANHEASDEVREKMEILDNNWTALLELWDERHRQYEQCLDFHLFYRDSEQVDSWMSRQEAFLENEDLGNSLGSAEALLQKHEDFEEAFTAQEEKIITVDKTATKLIGDDHYDSENIKAIRDGLLARRDALREKAATRRRLLKESLLLQKLYEDSDDLKNWINKKKKLADDEDYKDIQNLKSRVQKQQVFEKELAVNKTQLENIQKTGQEMIEGGHYASDNVTTRLSEVASLWEELLEATKQKGTQLHEANQQLQFENNAEDLQRWLEDVEWQVTSEDYGKGLAEVQNRLRKHGLLESAVAARQDQVDILTDLAAYFEEIGHPDSKDIRARQESLVCRFEALKEPLATRKKKLLDLLHLQLICRDTEDEEAWIQETEPSATSTYLGKDLIASKKLLNRHRVILENIASHEPRIQEITERGNKMVEEGHFAAEDVASRVKSLNQNMESLRARAARRQNDLEANVQFQQYLADLHEAETWIREKEPIVDNTNYGADEEAAGALLKKHEAFLLDLNSFGDSMKALRNQANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAADHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKVADDLLFEGLLTPEGAQIRQELNSRWGSLQRLADEQRQLLGSAHAVEVFHREADDTKEQIEKKCQALSAADPGSDLFSVQALQRRHEGFERDLVPLGDKVTILGETAERLSESHPDATEDLQRQKMELNEAWEDLQGRTKDRKESLNEAQKFYLFLSKARDLQNWISSIGGMVSSQELAEDLTGIEILLERHQEHRADMEAEAPTFQALEDFSAELIDSGHHASPEIEKKLQAVKLERDDLEKAWEKRKKILDQCLELQMFQGNCDQVESWMVARENSLRSDDKSSLDSLEALMKKRDDLDKAITAQEGKITDLEHFAESLIADEHYAKEEIATRLQRVLDRWKALKAQLIDERTKLGDYANLKQFYRDLEELEEWISEMLPTACDESYKDATNIQRKYLKHQTFAHEVDGRSEQVHGVINLGNSLIECSACDGNEEAMKEQLEQLKEHWDHLLERTNDKGKKLNEASRQQRFNTSIRDFEFWLSEAETLLAMKDQARDLASAGNLLKKHQLLEREMLAREDALKDLNTLAEDLLSSGTFNVDQIVKKKDNVNKRFLNVQELAAAHHEKLKEAYALFQFFQDLDDEESWIEEKLIRVSSQDYGRDLQGVQNLLKKHKRLEGELVAHEPAIQNVLDMAEKLKDKAAVGQEEIQLRLAQFVEHWEKLKELAKARGLKLEESLEYLQFMQNAEEEEAWINEKNALAVRGDCGDTLAATQSLLMKHEALENDFAVHETRVQNVCAQGEDILNKVLQEESQNKEISSKIEALNEKTPSLAKAIAAWKLQLEDDYAFQEFNWKADVVEAWIADKETSLKTNGNGADLGDFLTLLAKQDTLDASLQSFQQERLPEITDLKDKLISAQHNQSKAIEERYAALLKRWEQLLEASAVHRQKLLEKQLPLQKAEDLFVEFAHKASALNNWCEKMEENLSEPVHCVSLNEIRQLQKDHEDFLASLARAQADFKCLLELDQQIKALGVPSSPYTWLTVEVLERTWKHLSDIIEEREQELQKEEARQVKNFEMCQEFEQNASTFLQWILETRAYFLDGSLLKETGTLESQLEANKRKQKEIQAMKRQLTKIVDLGDNLEDALILDIKYSTIGLAQQWDQLYQLGLRMQHNLEQQIQAKDIKGVSEETLKEFSTIYKHFDENLTGRLTHKEFRSCLRGLNYYLPMVEEDEHEPKFEKFLDAVDPGRKGYVSLEDYTAFLIDKESENIKSSDEIENAFQALAEGKSYITKEDMKQALTPEQVSFCATHMQQYMDPRGRSHLSGYDYVGFTNSYFGN

Target ORF information:

RefSeq Version	NM_003126.4
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003126.4

ORF Insert Sequence:

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA 
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG 
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA 
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC 
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA 
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG 
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT 
CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA 
AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG 
GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG 
CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC 
CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT 
AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG 
AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG 
AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT 
GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC 
TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT 
ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT 
CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTA1 ( NM_003126.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003117.2 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA.

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CloneID	OHu28487
Clone ID Related Accession (Same CDS sequence)	NM_003126.4 , XM_011509916.2 , NM_003126.2 , NM_003126.3
Accession Version	NM_003126.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-27
Organism	Homo sapiens(Human)
Product	spectrin alpha chain, erythrocytic 1
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL353894.14, M61877.1, CD616351.1 and BU665064.1. This sequence is a reference standard in the RefSeqGene project. On Sep 22, 2006 this sequence version replaced NM_003126.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M61877.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA 
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG 
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA 
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC 
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA 
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG 
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT 
CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA 
AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG 
GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG 
CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC 
CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT 
AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG 
AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG 
AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT 
GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC 
TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT 
ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT 
CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003117.2
CDS	200..7459
Translation	MEQFPKETVVESSGPKVLETAEEIQERRQEVLTRYQSFKERVAERGQKLEDSYHLQVFKRDADDLGKWIMEKVNILTDKSYEDPTNIQGKYQKHQSLEAEVQTKSRLMSELEKTREERFTMGHSAHEETKAHIEELRHLWDLLLELTLEKGDQLLRALKFQQYVQECADILEWIGDKEAIATSVELGEDWERTEVLHKKFEDFQVELVAKEGRVVEVNQYANECAEENHPDLPLIQSKQNEVNAAWERLRGLALQRQKALSNAANLQRFKRDVTEAIQWIKEKEPVLTSEDYGKDLVASEGLFHSHKGLERNLAVMSDKVKELCAKAEKLTLSHPSDAPQIQEMKEDLVSSWEHIRALATSRYEKLQATYWYHRFSSDFDELSGWMNEKTAAINADELPTDVAGGEVLLDRHQQHKHEIDSYDDRFQSADETGQDLVNANHEASDEVREKMEILDNNWTALLELWDERHRQYEQCLDFHLFYRDSEQVDSWMSRQEAFLENEDLGNSLGSAEALLQKHEDFEEAFTAQEEKIITVDKTATKLIGDDHYDSENIKAIRDGLLARRDALREKAATRRRLLKESLLLQKLYEDSDDLKNWINKKKKLADDEDYKDIQNLKSRVQKQQVFEKELAVNKTQLENIQKTGQEMIEGGHYASDNVTTRLSEVASLWEELLEATKQKGTQLHEANQQLQFENNAEDLQRWLEDVEWQVTSEDYGKGLAEVQNRLRKHGLLESAVAARQDQVDILTDLAAYFEEIGHPDSKDIRARQESLVCRFEALKEPLATRKKKLLDLLHLQLICRDTEDEEAWIQETEPSATSTYLGKDLIASKKLLNRHRVILENIASHEPRIQEITERGNKMVEEGHFAAEDVASRVKSLNQNMESLRARAARRQNDLEANVQFQQYLADLHEAETWIREKEPIVDNTNYGADEEAAGALLKKHEAFLLDLNSFGDSMKALRNQANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAADHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKVADDLLFEGLLTPEGAQIRQELNSRWGSLQRLADEQRQLLGSAHAVEVFHREADDTKEQIEKKCQALSAADPGSDLFSVQALQRRHEGFERDLVPLGDKVTILGETAERLSESHPDATEDLQRQKMELNEAWEDLQGRTKDRKESLNEAQKFYLFLSKARDLQNWISSIGGMVSSQELAEDLTGIEILLERHQEHRADMEAEAPTFQALEDFSAELIDSGHHASPEIEKKLQAVKLERDDLEKAWEKRKKILDQCLELQMFQGNCDQVESWMVARENSLRSDDKSSLDSLEALMKKRDDLDKAITAQEGKITDLEHFAESLIADEHYAKEEIATRLQRVLDRWKALKAQLIDERTKLGDYANLKQFYRDLEELEEWISEMLPTACDESYKDATNIQRKYLKHQTFAHEVDGRSEQVHGVINLGNSLIECSACDGNEEAMKEQLEQLKEHWDHLLERTNDKGKKLNEASRQQRFNTSIRDFEFWLSEAETLLAMKDQARDLASAGNLLKKHQLLEREMLAREDALKDLNTLAEDLLSSGTFNVDQIVKKKDNVNKRFLNVQELAAAHHEKLKEAYALFQFFQDLDDEESWIEEKLIRVSSQDYGRDLQGVQNLLKKHKRLEGELVAHEPAIQNVLDMAEKLKDKAAVGQEEIQLRLAQFVEHWEKLKELAKARGLKLEESLEYLQFMQNAEEEEAWINEKNALAVRGDCGDTLAATQSLLMKHEALENDFAVHETRVQNVCAQGEDILNKVLQEESQNKEISSKIEALNEKTPSLAKAIAAWKLQLEDDYAFQEFNWKADVVEAWIADKETSLKTNGNGADLGDFLTLLAKQDTLDASLQSFQQERLPEITDLKDKLISAQHNQSKAIEERYAALLKRWEQLLEASAVHRQKLLEKQLPLQKAEDLFVEFAHKASALNNWCEKMEENLSEPVHCVSLNEIRQLQKDHEDFLASLARAQADFKCLLELDQQIKALGVPSSPYTWLTVEVLERTWKHLSDIIEEREQELQKEEARQVKNFEMCQEFEQNASTFLQWILETRAYFLDGSLLKETGTLESQLEANKRKQKEIQAMKRQLTKIVDLGDNLEDALILDIKYSTIGLAQQWDQLYQLGLRMQHNLEQQIQAKDIKGVSEETLKEFSTIYKHFDENLTGRLTHKEFRSCLRGLNYYLPMVEEDEHEPKFEKFLDAVDPGRKGYVSLEDYTAFLIDKESENIKSSDEIENAFQALAEGKSYITKEDMKQALTPEQVSFCATHMQQYMDPRGRSHLSGYDYVGFTNSYFGN

Target ORF information:

RefSeq Version	NM_003126.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003126.2

ORF Insert Sequence:

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA 
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG 
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA 
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC 
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA 
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG 
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT 
CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA 
AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG 
GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG 
CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC 
CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT 
AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG 
AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG 
AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT 
GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC 
TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT 
ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT 
CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTA1 ( NM_003126.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003117.2 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA.

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CloneID	OHu28487
Clone ID Related Accession (Same CDS sequence)	NM_003126.4 , XM_011509916.2 , NM_003126.2 , NM_003126.3
Accession Version	NM_003126.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-26
Organism	Homo sapiens(human)
Product	spectrin alpha chain, erythrocytic 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL353894.14, M61877.1, CD616351.1 and BU665064.1. This sequence is a reference standard in the RefSeqGene project. On Aug 24, 2017 this sequence version replaced NM_003126.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M61877.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1968189 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA 
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG 
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA 
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC 
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA 
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG 
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT 
CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA 
AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG 
GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG 
CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC 
CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT 
AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG 
AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG 
AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT 
GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC 
TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT 
ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT 
CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003117.2
CDS	199..7458
Translation	MEQFPKETVVESSGPKVLETAEEIQERRQEVLTRYQSFKERVAERGQKLEDSYHLQVFKRDADDLGKWIMEKVNILTDKSYEDPTNIQGKYQKHQSLEAEVQTKSRLMSELEKTREERFTMGHSAHEETKAHIEELRHLWDLLLELTLEKGDQLLRALKFQQYVQECADILEWIGDKEAIATSVELGEDWERTEVLHKKFEDFQVELVAKEGRVVEVNQYANECAEENHPDLPLIQSKQNEVNAAWERLRGLALQRQKALSNAANLQRFKRDVTEAIQWIKEKEPVLTSEDYGKDLVASEGLFHSHKGLERNLAVMSDKVKELCAKAEKLTLSHPSDAPQIQEMKEDLVSSWEHIRALATSRYEKLQATYWYHRFSSDFDELSGWMNEKTAAINADELPTDVAGGEVLLDRHQQHKHEIDSYDDRFQSADETGQDLVNANHEASDEVREKMEILDNNWTALLELWDERHRQYEQCLDFHLFYRDSEQVDSWMSRQEAFLENEDLGNSLGSAEALLQKHEDFEEAFTAQEEKIITVDKTATKLIGDDHYDSENIKAIRDGLLARRDALREKAATRRRLLKESLLLQKLYEDSDDLKNWINKKKKLADDEDYKDIQNLKSRVQKQQVFEKELAVNKTQLENIQKTGQEMIEGGHYASDNVTTRLSEVASLWEELLEATKQKGTQLHEANQQLQFENNAEDLQRWLEDVEWQVTSEDYGKGLAEVQNRLRKHGLLESAVAARQDQVDILTDLAAYFEEIGHPDSKDIRARQESLVCRFEALKEPLATRKKKLLDLLHLQLICRDTEDEEAWIQETEPSATSTYLGKDLIASKKLLNRHRVILENIASHEPRIQEITERGNKMVEEGHFAAEDVASRVKSLNQNMESLRARAARRQNDLEANVQFQQYLADLHEAETWIREKEPIVDNTNYGADEEAAGALLKKHEAFLLDLNSFGDSMKALRNQANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAADHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKVADDLLFEGLLTPEGAQIRQELNSRWGSLQRLADEQRQLLGSAHAVEVFHREADDTKEQIEKKCQALSAADPGSDLFSVQALQRRHEGFERDLVPLGDKVTILGETAERLSESHPDATEDLQRQKMELNEAWEDLQGRTKDRKESLNEAQKFYLFLSKARDLQNWISSIGGMVSSQELAEDLTGIEILLERHQEHRADMEAEAPTFQALEDFSAELIDSGHHASPEIEKKLQAVKLERDDLEKAWEKRKKILDQCLELQMFQGNCDQVESWMVARENSLRSDDKSSLDSLEALMKKRDDLDKAITAQEGKITDLEHFAESLIADEHYAKEEIATRLQRVLDRWKALKAQLIDERTKLGDYANLKQFYRDLEELEEWISEMLPTACDESYKDATNIQRKYLKHQTFAHEVDGRSEQVHGVINLGNSLIECSACDGNEEAMKEQLEQLKEHWDHLLERTNDKGKKLNEASRQQRFNTSIRDFEFWLSEAETLLAMKDQARDLASAGNLLKKHQLLEREMLAREDALKDLNTLAEDLLSSGTFNVDQIVKKKDNVNKRFLNVQELAAAHHEKLKEAYALFQFFQDLDDEESWIEEKLIRVSSQDYGRDLQGVQNLLKKHKRLEGELVAHEPAIQNVLDMAEKLKDKAAVGQEEIQLRLAQFVEHWEKLKELAKARGLKLEESLEYLQFMQNAEEEEAWINEKNALAVRGDCGDTLAATQSLLMKHEALENDFAVHETRVQNVCAQGEDILNKVLQEESQNKEISSKIEALNEKTPSLAKAIAAWKLQLEDDYAFQEFNWKADVVEAWIADKETSLKTNGNGADLGDFLTLLAKQDTLDASLQSFQQERLPEITDLKDKLISAQHNQSKAIEERYAALLKRWEQLLEASAVHRQKLLEKQLPLQKAEDLFVEFAHKASALNNWCEKMEENLSEPVHCVSLNEIRQLQKDHEDFLASLARAQADFKCLLELDQQIKALGVPSSPYTWLTVEVLERTWKHLSDIIEEREQELQKEEARQVKNFEMCQEFEQNASTFLQWILETRAYFLDGSLLKETGTLESQLEANKRKQKEIQAMKRQLTKIVDLGDNLEDALILDIKYSTIGLAQQWDQLYQLGLRMQHNLEQQIQAKDIKGVSEETLKEFSTIYKHFDENLTGRLTHKEFRSCLRGLNYYLPMVEEDEHEPKFEKFLDAVDPGRKGYVSLEDYTAFLIDKESENIKSSDEIENAFQALAEGKSYITKEDMKQALTPEQVSFCATHMQQYMDPRGRSHLSGYDYVGFTNSYFGN

Target ORF information:

RefSeq Version	NM_003126.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003126.3

ORF Insert Sequence:

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA 
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG 
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA 
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC 
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA 
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG 
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT 
CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA 
AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG 
GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG 
CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC 
CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT 
AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG 
AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG 
AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT 
GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC 
TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT 
ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT 
CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTA1 ( XM_011509916.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508218.1 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA.

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CloneID	OHu28487
Clone ID Related Accession (Same CDS sequence)	NM_003126.4 , XM_011509916.2 , NM_003126.2 , NM_003126.3
Accession Version	XM_011509916.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7260bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, erythrocytic 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509916.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA 
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG 
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA 
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC 
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA 
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG 
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT 
CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA 
AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG 
GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG 
CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC 
CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT 
AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG 
AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG 
AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT 
GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC 
TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT 
ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT 
CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508218.1
CDS	78..7337
Translation	MEQFPKETVVESSGPKVLETAEEIQERRQEVLTRYQSFKERVAERGQKLEDSYHLQVFKRDADDLGKWIMEKVNILTDKSYEDPTNIQGKYQKHQSLEAEVQTKSRLMSELEKTREERFTMGHSAHEETKAHIEELRHLWDLLLELTLEKGDQLLRALKFQQYVQECADILEWIGDKEAIATSVELGEDWERTEVLHKKFEDFQVELVAKEGRVVEVNQYANECAEENHPDLPLIQSKQNEVNAAWERLRGLALQRQKALSNAANLQRFKRDVTEAIQWIKEKEPVLTSEDYGKDLVASEGLFHSHKGLERNLAVMSDKVKELCAKAEKLTLSHPSDAPQIQEMKEDLVSSWEHIRALATSRYEKLQATYWYHRFSSDFDELSGWMNEKTAAINADELPTDVAGGEVLLDRHQQHKHEIDSYDDRFQSADETGQDLVNANHEASDEVREKMEILDNNWTALLELWDERHRQYEQCLDFHLFYRDSEQVDSWMSRQEAFLENEDLGNSLGSAEALLQKHEDFEEAFTAQEEKIITVDKTATKLIGDDHYDSENIKAIRDGLLARRDALREKAATRRRLLKESLLLQKLYEDSDDLKNWINKKKKLADDEDYKDIQNLKSRVQKQQVFEKELAVNKTQLENIQKTGQEMIEGGHYASDNVTTRLSEVASLWEELLEATKQKGTQLHEANQQLQFENNAEDLQRWLEDVEWQVTSEDYGKGLAEVQNRLRKHGLLESAVAARQDQVDILTDLAAYFEEIGHPDSKDIRARQESLVCRFEALKEPLATRKKKLLDLLHLQLICRDTEDEEAWIQETEPSATSTYLGKDLIASKKLLNRHRVILENIASHEPRIQEITERGNKMVEEGHFAAEDVASRVKSLNQNMESLRARAARRQNDLEANVQFQQYLADLHEAETWIREKEPIVDNTNYGADEEAAGALLKKHEAFLLDLNSFGDSMKALRNQANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAADHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKVADDLLFEGLLTPEGAQIRQELNSRWGSLQRLADEQRQLLGSAHAVEVFHREADDTKEQIEKKCQALSAADPGSDLFSVQALQRRHEGFERDLVPLGDKVTILGETAERLSESHPDATEDLQRQKMELNEAWEDLQGRTKDRKESLNEAQKFYLFLSKARDLQNWISSIGGMVSSQELAEDLTGIEILLERHQEHRADMEAEAPTFQALEDFSAELIDSGHHASPEIEKKLQAVKLERDDLEKAWEKRKKILDQCLELQMFQGNCDQVESWMVARENSLRSDDKSSLDSLEALMKKRDDLDKAITAQEGKITDLEHFAESLIADEHYAKEEIATRLQRVLDRWKALKAQLIDERTKLGDYANLKQFYRDLEELEEWISEMLPTACDESYKDATNIQRKYLKHQTFAHEVDGRSEQVHGVINLGNSLIECSACDGNEEAMKEQLEQLKEHWDHLLERTNDKGKKLNEASRQQRFNTSIRDFEFWLSEAETLLAMKDQARDLASAGNLLKKHQLLEREMLAREDALKDLNTLAEDLLSSGTFNVDQIVKKKDNVNKRFLNVQELAAAHHEKLKEAYALFQFFQDLDDEESWIEEKLIRVSSQDYGRDLQGVQNLLKKHKRLEGELVAHEPAIQNVLDMAEKLKDKAAVGQEEIQLRLAQFVEHWEKLKELAKARGLKLEESLEYLQFMQNAEEEEAWINEKNALAVRGDCGDTLAATQSLLMKHEALENDFAVHETRVQNVCAQGEDILNKVLQEESQNKEISSKIEALNEKTPSLAKAIAAWKLQLEDDYAFQEFNWKADVVEAWIADKETSLKTNGNGADLGDFLTLLAKQDTLDASLQSFQQERLPEITDLKDKLISAQHNQSKAIEERYAALLKRWEQLLEASAVHRQKLLEKQLPLQKAEDLFVEFAHKASALNNWCEKMEENLSEPVHCVSLNEIRQLQKDHEDFLASLARAQADFKCLLELDQQIKALGVPSSPYTWLTVEVLERTWKHLSDIIEEREQELQKEEARQVKNFEMCQEFEQNASTFLQWILETRAYFLDGSLLKETGTLESQLEANKRKQKEIQAMKRQLTKIVDLGDNLEDALILDIKYSTIGLAQQWDQLYQLGLRMQHNLEQQIQAKDIKGVSEETLKEFSTIYKHFDENLTGRLTHKEFRSCLRGLNYYLPMVEEDEHEPKFEKFLDAVDPGRKGYVSLEDYTAFLIDKESENIKSSDEIENAFQALAEGKSYITKEDMKQALTPEQVSFCATHMQQYMDPRGRSHLSGYDYVGFTNSYFGN

Target ORF information:

RefSeq Version	XM_011509916.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509916.2

ORF Insert Sequence:

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA 
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG 
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA 
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC 
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA 
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG 
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG GGCTTACTTT 
CTGGATGGAT CATTGCTCAA AGAAACAGGA ACTCTGGAAT CTCAGCTGGA AGCAAATAAA 
AGAAAACAGA AGGAGATCCA GGCGATGAAG CGTCAACTAA CCAAGATTGT GGACCTGGGG 
GACAACTTGG AAGACGCTCT GATCCTTGAT ATCAAATACA GCACCATTGG ATTGGCTCAG 
CAGTGGGACC AGCTCTACCA GCTTGGGTTG CGGATGCAAC ACAACCTGGA GCAACAGATC 
CAGGCCAAGG ACATCAAAGG TGTGAGTGAA GAGACTCTAA AGGAATTTAG CACAATCTAT 
AAACACTTTG ATGAGAATTT GACAGGGCGC CTGACTCACA AAGAGTTCCG GTCCTGCCTG 
AGAGGACTCA ATTACTACTT GCCCATGGTG GAGGAGGATG AACATGAGCC CAAGTTTGAG 
AAGTTCCTGG ATGCTGTGGA TCCAGGGAGG AAGGGCTATG TCTCACTGGA GGACTATACT 
GCTTTCCTGA TTGACAAGGA GTCAGAAAAC ATCAAGTCCA GTGATGAAAT AGAGAATGCC 
TTCCAAGCCC TGGCAGAGGG CAAGTCATAT ATTACCAAAG AAGACATGAA GCAGGCCCTT 
ACCCCAGAGC AAGTGTCATT CTGTGCCACA CATATGCAGC AATATATGGA CCCACGGGGT 
CGAAGCCATC TCTCTGGCTA TGACTACGTT GGCTTCACCA ATTCCTACTT TGGCAACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTA1 ( XM_011509917.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508219.1 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.

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CloneID	OHu60716
Clone ID Related Accession (Same CDS sequence)	XM_011509917.3 , XM_011509917.2
Accession Version	XM_011509917.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509917.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA 
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG 
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA 
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC 
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA 
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG 
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC 
AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC 
CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT 
CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC 
CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACATCAAA 
GGTGTGAGTG AAGAGACTCT AAAGGAATTT AGCACAATCT ATAAACACTT TGATGAGAAT 
TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC 
TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG 
GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG 
GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCAGAG 
GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA 
TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC 
TATGACTACG TTGGCTTCAC CAATTCCTAC TTTGGCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508219.1
CDS	126..7367
Translation	MEQFPKETVVESSGPKVLETAEEIQERRQEVLTRYQSFKERVAERGQKLEDSYHLQVFKRDADDLGKWIMEKVNILTDKSYEDPTNIQGKYQKHQSLEAEVQTKSRLMSELEKTREERFTMGHSAHEETKAHIEELRHLWDLLLELTLEKGDQLLRALKFQQYVQECADILEWIGDKEAIATSVELGEDWERTEVLHKKFEDFQVELVAKEGRVVEVNQYANECAEENHPDLPLIQSKQNEVNAAWERLRGLALQRQKALSNAANLQRFKRDVTEAIQWIKEKEPVLTSEDYGKDLVASEGLFHSHKGLERNLAVMSDKVKELCAKAEKLTLSHPSDAPQIQEMKEDLVSSWEHIRALATSRYEKLQATYWYHRFSSDFDELSGWMNEKTAAINADELPTDVAGGEVLLDRHQQHKHEIDSYDDRFQSADETGQDLVNANHEASDEVREKMEILDNNWTALLELWDERHRQYEQCLDFHLFYRDSEQVDSWMSRQEAFLENEDLGNSLGSAEALLQKHEDFEEAFTAQEEKIITVDKTATKLIGDDHYDSENIKAIRDGLLARRDALREKAATRRRLLKESLLLQKLYEDSDDLKNWINKKKKLADDEDYKDIQNLKSRVQKQQVFEKELAVNKTQLENIQKTGQEMIEGGHYASDNVTTRLSEVASLWEELLEATKQKGTQLHEANQQLQFENNAEDLQRWLEDVEWQVTSEDYGKGLAEVQNRLRKHGLLESAVAARQDQVDILTDLAAYFEEIGHPDSKDIRARQESLVCRFEALKEPLATRKKKLLDLLHLQLICRDTEDEEAWIQETEPSATSTYLGKDLIASKKLLNRHRVILENIASHEPRIQEITERGNKMVEEGHFAAEDVASRVKSLNQNMESLRARAARRQNDLEANVQFQQYLADLHEAETWIREKEPIVDNTNYGADEEAAGALLKKHEAFLLDLNSFGDSMKALRNQANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAADHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKVADDLLFEGLLTPEGAQIRQELNSRWGSLQRLADEQRQLLGSAHAVEVFHREADDTKEQIEKKCQALSAADPGSDLFSVQALQRRHEGFERDLVPLGDKVTILGETAERLSESHPDATEDLQRQKMELNEAWEDLQGRTKDRKESLNEAQKFYLFLSKARDLQNWISSIGGMVSSQELAEDLTGIEILLERHQEHRADMEAEAPTFQALEDFSAELIDSGHHASPEIEKKLQAVKLERDDLEKAWEKRKKILDQCLELQMFQGNCDQVESWMVARENSLRSDDKSSLDSLEALMKKRDDLDKAITAQEGKITDLEHFAESLIADEHYAKEEIATRLQRVLDRWKALKAQLIDERTKLGDYANLKQFYRDLEELEEWISEMLPTACDESYKDATNIQRKYLKHQTFAHEVDGRSEQVHGVINLGNSLIECSACDGNEEAMKEQLEQLKEHWDHLLERTNDKGKKLNEASRQQRFNTSIRDFEFWLSEAETLLAMKDQARDLASAGNLLKKHQLLEREMLAREDALKDLNTLAEDLLSSGTFNVDQIVKKKDNVNKRFLNVQELAAAHHEKLKEAYALFQFFQDLDDEESWIEEKLIRVSSQDYGRDLQGVQNLLKKHKRLEGELVAHEPAIQNVLDMAEKLKDKAAVGQEEIQLRLAQFVEHWEKLKELAKARGLKLEESLEYLQFMQNAEEEEAWINEKNALAVRGDCGDTLAATQSLLMKHEALENDFAVHETRVQNVCAQGEDILNKVLQEESQNKEISSKIEALNEKTPSLAKAIAAWKLQLEDDYAFQEFNWKADVVEAWIADKETSLKTNGNGADLGDFLTLLAKQDTLDASLQSFQQERLPEITDLKDKLISAQHNQSKAIEERYAALLKRWEQLLEASAVHRQKLLEKQLPLQKAEDLFVEFAHKASALNNWCEKMEENLSEPVHCVSLNEIRQLQKDHEDFLASLARAQADFKCLLELDQQIKALGVPSSPYTWLTVEVLERTWKHLSDIIEEREQELQKEEARQVKNFEMCQEFEQNASTFLQWILETRSLLKETGTLESQLEANKRKQKEIQAMKRQLTKIVDLGDNLEDALILDIKYSTIGLAQQWDQLYQLGLRMQHNLEQQIQAKDIKGVSEETLKEFSTIYKHFDENLTGRLTHKEFRSCLRGLNYYLPMVEEDEHEPKFEKFLDAVDPGRKGYVSLEDYTAFLIDKESENIKSSDEIENAFQALAEGKSYITKEDMKQALTPEQVSFCATHMQQYMDPRGRSHLSGYDYVGFTNSYFGN

Target ORF information:

RefSeq Version	XM_011509917.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509917.3

ORF Insert Sequence:

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA 
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG 
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA 
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC 
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA 
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG 
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC 
AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC 
CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT 
CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC 
CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACATCAAA 
GGTGTGAGTG AAGAGACTCT AAAGGAATTT AGCACAATCT ATAAACACTT TGATGAGAAT 
TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC 
TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG 
GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG 
GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCAGAG 
GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA 
TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC 
TATGACTACG TTGGCTTCAC CAATTCCTAC TTTGGCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTA1 ( XM_011509917.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508219.1 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.

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CloneID	OHu60716
Clone ID Related Accession (Same CDS sequence)	XM_011509917.3 , XM_011509917.2
Accession Version	XM_011509917.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, erythrocytic 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509917.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA 
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG 
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA 
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC 
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA 
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG 
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC 
AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC 
CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT 
CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC 
CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACATCAAA 
GGTGTGAGTG AAGAGACTCT AAAGGAATTT AGCACAATCT ATAAACACTT TGATGAGAAT 
TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC 
TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG 
GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG 
GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCAGAG 
GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA 
TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC 
TATGACTACG TTGGCTTCAC CAATTCCTAC TTTGGCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508219.1
CDS	114..7355
Translation	MEQFPKETVVESSGPKVLETAEEIQERRQEVLTRYQSFKERVAERGQKLEDSYHLQVFKRDADDLGKWIMEKVNILTDKSYEDPTNIQGKYQKHQSLEAEVQTKSRLMSELEKTREERFTMGHSAHEETKAHIEELRHLWDLLLELTLEKGDQLLRALKFQQYVQECADILEWIGDKEAIATSVELGEDWERTEVLHKKFEDFQVELVAKEGRVVEVNQYANECAEENHPDLPLIQSKQNEVNAAWERLRGLALQRQKALSNAANLQRFKRDVTEAIQWIKEKEPVLTSEDYGKDLVASEGLFHSHKGLERNLAVMSDKVKELCAKAEKLTLSHPSDAPQIQEMKEDLVSSWEHIRALATSRYEKLQATYWYHRFSSDFDELSGWMNEKTAAINADELPTDVAGGEVLLDRHQQHKHEIDSYDDRFQSADETGQDLVNANHEASDEVREKMEILDNNWTALLELWDERHRQYEQCLDFHLFYRDSEQVDSWMSRQEAFLENEDLGNSLGSAEALLQKHEDFEEAFTAQEEKIITVDKTATKLIGDDHYDSENIKAIRDGLLARRDALREKAATRRRLLKESLLLQKLYEDSDDLKNWINKKKKLADDEDYKDIQNLKSRVQKQQVFEKELAVNKTQLENIQKTGQEMIEGGHYASDNVTTRLSEVASLWEELLEATKQKGTQLHEANQQLQFENNAEDLQRWLEDVEWQVTSEDYGKGLAEVQNRLRKHGLLESAVAARQDQVDILTDLAAYFEEIGHPDSKDIRARQESLVCRFEALKEPLATRKKKLLDLLHLQLICRDTEDEEAWIQETEPSATSTYLGKDLIASKKLLNRHRVILENIASHEPRIQEITERGNKMVEEGHFAAEDVASRVKSLNQNMESLRARAARRQNDLEANVQFQQYLADLHEAETWIREKEPIVDNTNYGADEEAAGALLKKHEAFLLDLNSFGDSMKALRNQANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAADHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKVADDLLFEGLLTPEGAQIRQELNSRWGSLQRLADEQRQLLGSAHAVEVFHREADDTKEQIEKKCQALSAADPGSDLFSVQALQRRHEGFERDLVPLGDKVTILGETAERLSESHPDATEDLQRQKMELNEAWEDLQGRTKDRKESLNEAQKFYLFLSKARDLQNWISSIGGMVSSQELAEDLTGIEILLERHQEHRADMEAEAPTFQALEDFSAELIDSGHHASPEIEKKLQAVKLERDDLEKAWEKRKKILDQCLELQMFQGNCDQVESWMVARENSLRSDDKSSLDSLEALMKKRDDLDKAITAQEGKITDLEHFAESLIADEHYAKEEIATRLQRVLDRWKALKAQLIDERTKLGDYANLKQFYRDLEELEEWISEMLPTACDESYKDATNIQRKYLKHQTFAHEVDGRSEQVHGVINLGNSLIECSACDGNEEAMKEQLEQLKEHWDHLLERTNDKGKKLNEASRQQRFNTSIRDFEFWLSEAETLLAMKDQARDLASAGNLLKKHQLLEREMLAREDALKDLNTLAEDLLSSGTFNVDQIVKKKDNVNKRFLNVQELAAAHHEKLKEAYALFQFFQDLDDEESWIEEKLIRVSSQDYGRDLQGVQNLLKKHKRLEGELVAHEPAIQNVLDMAEKLKDKAAVGQEEIQLRLAQFVEHWEKLKELAKARGLKLEESLEYLQFMQNAEEEEAWINEKNALAVRGDCGDTLAATQSLLMKHEALENDFAVHETRVQNVCAQGEDILNKVLQEESQNKEISSKIEALNEKTPSLAKAIAAWKLQLEDDYAFQEFNWKADVVEAWIADKETSLKTNGNGADLGDFLTLLAKQDTLDASLQSFQQERLPEITDLKDKLISAQHNQSKAIEERYAALLKRWEQLLEASAVHRQKLLEKQLPLQKAEDLFVEFAHKASALNNWCEKMEENLSEPVHCVSLNEIRQLQKDHEDFLASLARAQADFKCLLELDQQIKALGVPSSPYTWLTVEVLERTWKHLSDIIEEREQELQKEEARQVKNFEMCQEFEQNASTFLQWILETRSLLKETGTLESQLEANKRKQKEIQAMKRQLTKIVDLGDNLEDALILDIKYSTIGLAQQWDQLYQLGLRMQHNLEQQIQAKDIKGVSEETLKEFSTIYKHFDENLTGRLTHKEFRSCLRGLNYYLPMVEEDEHEPKFEKFLDAVDPGRKGYVSLEDYTAFLIDKESENIKSSDEIENAFQALAEGKSYITKEDMKQALTPEQVSFCATHMQQYMDPRGRSHLSGYDYVGFTNSYFGN

Target ORF information:

RefSeq Version	XM_011509917.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509917.2

ORF Insert Sequence:

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GCTGAGGACC TGTTCGTGGA ATTTGCACAT AAGGCTTCAG CTTTGAACAA CTGGTGTGAA 
AAGATGGAAG AAAACTTGTC AGAGCCTGTG CACTGTGTCT CCCTGAATGA AATTCGGCAG 
CTGCAGAAAG ACCATGAGGA CTTCTTGGCC TCCCTGGCTA GGGCTCAAGC AGACTTTAAA 
TGTTTGCTGG AGCTAGACCA GCAGATTAAG GCCTTAGGTG TGCCTTCCAG CCCTTATACC 
TGGTTAACAG TGGAGGTGCT GGAAAGGACC TGGAAGCACC TATCTGACAT CATTGAGGAA 
CGGGAGCAGG AGCTGCAAAA GGAAGAGGCA AGACAGGTCA AGAACTTTGA GATGTGTCAG 
GAGTTTGAAC AGAATGCCAG TACCTTCCTT CAATGGATCC TGGAAACCAG ATCATTGCTC 
AAAGAAACAG GAACTCTGGA ATCTCAGCTG GAAGCAAATA AAAGAAAACA GAAGGAGATC 
CAGGCGATGA AGCGTCAACT AACCAAGATT GTGGACCTGG GGGACAACTT GGAAGACGCT 
CTGATCCTTG ATATCAAATA CAGCACCATT GGATTGGCTC AGCAGTGGGA CCAGCTCTAC 
CAGCTTGGGT TGCGGATGCA ACACAACCTG GAGCAACAGA TCCAGGCCAA GGACATCAAA 
GGTGTGAGTG AAGAGACTCT AAAGGAATTT AGCACAATCT ATAAACACTT TGATGAGAAT 
TTGACAGGGC GCCTGACTCA CAAAGAGTTC CGGTCCTGCC TGAGAGGACT CAATTACTAC 
TTGCCCATGG TGGAGGAGGA TGAACATGAG CCCAAGTTTG AGAAGTTCCT GGATGCTGTG 
GATCCAGGGA GGAAGGGCTA TGTCTCACTG GAGGACTATA CTGCTTTCCT GATTGACAAG 
GAGTCAGAAA ACATCAAGTC CAGTGATGAA ATAGAGAATG CCTTCCAAGC CCTGGCAGAG 
GGCAAGTCAT ATATTACCAA AGAAGACATG AAGCAGGCCC TTACCCCAGA GCAAGTGTCA 
TTCTGTGCCA CACATATGCA GCAATATATG GACCCACGGG GTCGAAGCCA TCTCTCTGGC 
TATGACTACG TTGGCTTCAC CAATTCCTAC TTTGGCAACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTA1 ( XM_011509918.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508220.1 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA.

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CloneID	OHu60717
Clone ID Related Accession (Same CDS sequence)	XM_011509918.2 , XM_011509918.3
Accession Version	XM_011509918.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011509918.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508220.1
CDS	114..6239
Translation	MEQFPKETVVESSGPKVLETAEEIQERRQEVLTRYQSFKERVAERGQKLEDSYHLQVFKRDADDLGKWIMEKVNILTDKSYEDPTNIQGKYQKHQSLEAEVQTKSRLMSELEKTREERFTMGHSAHEETKAHIEELRHLWDLLLELTLEKGDQLLRALKFQQYVQECADILEWIGDKEAIATSVELGEDWERTEVLHKKFEDFQVELVAKEGRVVEVNQYANECAEENHPDLPLIQSKQNEVNAAWERLRGLALQRQKALSNAANLQRFKRDVTEAIQWIKEKEPVLTSEDYGKDLVASEGLFHSHKGLERNLAVMSDKVKELCAKAEKLTLSHPSDAPQIQEMKEDLVSSWEHIRALATSRYEKLQATYWYHRFSSDFDELSGWMNEKTAAINADELPTDVAGGEVLLDRHQQHKHEIDSYDDRFQSADETGQDLVNANHEASDEVREKMEILDNNWTALLELWDERHRQYEQCLDFHLFYRDSEQVDSWMSRQEAFLENEDLGNSLGSAEALLQKHEDFEEAFTAQEEKIITVDKTATKLIGDDHYDSENIKAIRDGLLARRDALREKAATRRRLLKESLLLQKLYEDSDDLKNWINKKKKLADDEDYKDIQNLKSRVQKQQVFEKELAVNKTQLENIQKTGQEMIEGGHYASDNVTTRLSEVASLWEELLEATKQKGTQLHEANQQLQFENNAEDLQRWLEDVEWQVTSEDYGKGLAEVQNRLRKHGLLESAVAARQDQVDILTDLAAYFEEIGHPDSKDIRARQESLVCRFEALKEPLATRKKKLLDLLHLQLICRDTEDEEAWIQETEPSATSTYLGKDLIASKKLLNRHRVILENIASHEPRIQEITERGNKMVEEGHFAAEDVASRVKSLNQNMESLRARAARRQNDLEANVQFQQYLADLHEAETWIREKEPIVDNTNYGADEEAAGALLKKHEAFLLDLNSFGDSMKALRNQANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAADHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKVADDLLFEGLLTPEGAQIRQELNSRWGSLQRLADEQRQLLGSAHAVEVFHREADDTKEQIEKKCQALSAADPGSDLFSVQALQRRHEGFERDLVPLGDKVTILGETAERLSESHPDATEDLQRQKMELNEAWEDLQGRTKDRKESLNEAQKFYLFLSKARDLQNWISSIGGMVSSQELAEDLTGIEILLERHQEHRADMEAEAPTFQALEDFSAELIDSGHHASPEIEKKLQAVKLERDDLEKAWEKRKKILDQCLELQMFQGNCDQVESWMVARENSLRSDDKSSLDSLEALMKKRDDLDKAITAQEGKITDLEHFAESLIADEHYAKEEIATRLQRVLDRWKALKAQLIDERTKLGDYANLKQFYRDLEELEEWISEMLPTACDESYKDATNIQRKYLKHQTFAHEVDGRSEQVHGVINLGNSLIECSACDGNEEAMKEQLEQLKEHWDHLLERTNDKGKKLNEASRQQRFNTSIRDFEFWLSEAETLLAMKDQARDLASAGNLLKKHQLLEREMLAREDALKDLNTLAEDLLSSGTFNVDQIVKKKDNVNKRFLNVQELAAAHHEKLKEAYALFQFFQDLDDEESWIEEKLIRVSSQDYGRDLQGVQNLLKKHKRLEGELVAHEPAIQNVLDMAEKLKDKAAVGQEEIQLRLAQFVEHWEKLKELAKARGLKLEESLEYLQFMQNAEEEEAWINEKNALAVRGDCGDTLAATQSLLMKHEALENDFAVHETRVQNVCAQGEDILNKVLQEESQNKEISSKIEALNEKTPSLAKAIAAWKLQLEDDYAFQEFNWKADVVEAWIADKETSLKTNGNGADLGDFLTLLAKQDTLDASLQSFQQERLPEITDLKDKLISAQHNQSKAIEERYAALLKRWEQLLEASAVHRQKLLEKQLPLQKV

Target ORF information:

RefSeq Version	XM_011509918.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509918.2

ORF Insert Sequence:

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPTA1 ( XM_011509918.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011508220.1 Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA.

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CloneID	OHu60717
Clone ID Related Accession (Same CDS sequence)	XM_011509918.2 , XM_011509918.3
Accession Version	XM_011509918.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6126bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spectrin alpha chain, erythrocytic 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000001.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011509918.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011508220.1
CDS	126..6251
Translation	MEQFPKETVVESSGPKVLETAEEIQERRQEVLTRYQSFKERVAERGQKLEDSYHLQVFKRDADDLGKWIMEKVNILTDKSYEDPTNIQGKYQKHQSLEAEVQTKSRLMSELEKTREERFTMGHSAHEETKAHIEELRHLWDLLLELTLEKGDQLLRALKFQQYVQECADILEWIGDKEAIATSVELGEDWERTEVLHKKFEDFQVELVAKEGRVVEVNQYANECAEENHPDLPLIQSKQNEVNAAWERLRGLALQRQKALSNAANLQRFKRDVTEAIQWIKEKEPVLTSEDYGKDLVASEGLFHSHKGLERNLAVMSDKVKELCAKAEKLTLSHPSDAPQIQEMKEDLVSSWEHIRALATSRYEKLQATYWYHRFSSDFDELSGWMNEKTAAINADELPTDVAGGEVLLDRHQQHKHEIDSYDDRFQSADETGQDLVNANHEASDEVREKMEILDNNWTALLELWDERHRQYEQCLDFHLFYRDSEQVDSWMSRQEAFLENEDLGNSLGSAEALLQKHEDFEEAFTAQEEKIITVDKTATKLIGDDHYDSENIKAIRDGLLARRDALREKAATRRRLLKESLLLQKLYEDSDDLKNWINKKKKLADDEDYKDIQNLKSRVQKQQVFEKELAVNKTQLENIQKTGQEMIEGGHYASDNVTTRLSEVASLWEELLEATKQKGTQLHEANQQLQFENNAEDLQRWLEDVEWQVTSEDYGKGLAEVQNRLRKHGLLESAVAARQDQVDILTDLAAYFEEIGHPDSKDIRARQESLVCRFEALKEPLATRKKKLLDLLHLQLICRDTEDEEAWIQETEPSATSTYLGKDLIASKKLLNRHRVILENIASHEPRIQEITERGNKMVEEGHFAAEDVASRVKSLNQNMESLRARAARRQNDLEANVQFQQYLADLHEAETWIREKEPIVDNTNYGADEEAAGALLKKHEAFLLDLNSFGDSMKALRNQANACQQQQAAPVEGVAGEQRVMALYDFQARSPREVTMKKGDVLTLLSSINKDWWKVEAADHQGIVPAVYVRRLAHDEFPMLPQRRREEPGNITQRQEQIENQYRSLLDRAEERRRRLLQRYNEFLLAYEAGDMLEWIQEKKAENTGVELDDVWELQKKFDEFQKDLNTNEPRLRDINKVADDLLFEGLLTPEGAQIRQELNSRWGSLQRLADEQRQLLGSAHAVEVFHREADDTKEQIEKKCQALSAADPGSDLFSVQALQRRHEGFERDLVPLGDKVTILGETAERLSESHPDATEDLQRQKMELNEAWEDLQGRTKDRKESLNEAQKFYLFLSKARDLQNWISSIGGMVSSQELAEDLTGIEILLERHQEHRADMEAEAPTFQALEDFSAELIDSGHHASPEIEKKLQAVKLERDDLEKAWEKRKKILDQCLELQMFQGNCDQVESWMVARENSLRSDDKSSLDSLEALMKKRDDLDKAITAQEGKITDLEHFAESLIADEHYAKEEIATRLQRVLDRWKALKAQLIDERTKLGDYANLKQFYRDLEELEEWISEMLPTACDESYKDATNIQRKYLKHQTFAHEVDGRSEQVHGVINLGNSLIECSACDGNEEAMKEQLEQLKEHWDHLLERTNDKGKKLNEASRQQRFNTSIRDFEFWLSEAETLLAMKDQARDLASAGNLLKKHQLLEREMLAREDALKDLNTLAEDLLSSGTFNVDQIVKKKDNVNKRFLNVQELAAAHHEKLKEAYALFQFFQDLDDEESWIEEKLIRVSSQDYGRDLQGVQNLLKKHKRLEGELVAHEPAIQNVLDMAEKLKDKAAVGQEEIQLRLAQFVEHWEKLKELAKARGLKLEESLEYLQFMQNAEEEEAWINEKNALAVRGDCGDTLAATQSLLMKHEALENDFAVHETRVQNVCAQGEDILNKVLQEESQNKEISSKIEALNEKTPSLAKAIAAWKLQLEDDYAFQEFNWKADVVEAWIADKETSLKTNGNGADLGDFLTLLAKQDTLDASLQSFQQERLPEITDLKDKLISAQHNQSKAIEERYAALLKRWEQLLEASAVHRQKLLEKQLPLQKV

Target ORF information:

RefSeq Version	XM_011509918.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spectrin alpha, erythrocytic 1 (SPTA1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011509918.3

ORF Insert Sequence:

ATGGAGCAAT TTCCAAAGGA AACCGTTGTG GAGAGCAGTG GGCCAAAGGT TTTGGAAACA 
GCAGAAGAGA TCCAGGAGAG GCGTCAGGAA GTGTTGACTC GGTATCAAAG TTTCAAGGAG 
CGGGTCGCTG AGAGGGGTCA GAAGCTTGAG GATTCCTATC ACTTACAAGT TTTCAAGCGA 
GATGCAGATG ATCTGGGGAA GTGGATCATG GAGAAAGTCA ATATCTTAAC CGATAAGAGC 
TATGAAGACC CAACTAATAT ACAGGGGAAA TATCAGAAGC ATCAATCCCT TGAAGCAGAG 
GTGCAAACAA AATCAAGACT CATGTCTGAA CTGGAAAAAA CAAGGGAAGA ACGATTTACC 
ATGGGTCATT CTGCCCACGA AGAAACGAAG GCCCATATAG AGGAGCTACG CCACCTGTGG 
GACCTGCTGT TAGAGCTGAC CCTGGAGAAG GGTGACCAGT TGCTGCGGGC CCTGAAGTTC 
CAGCAGTATG TACAGGAGTG TGCTGACATC TTAGAGTGGA TTGGAGACAA GGAGGCTATA 
GCGACATCAG TGGAGCTAGG TGAAGACTGG GAGCGCACCG AAGTTCTGCA TAAGAAATTT 
GAAGACTTCC AAGTGGAGCT GGTAGCTAAA GAAGGGAGAG TTGTTGAAGT GAACCAATAT 
GCCAATGAGT GTGCCGAGGA AAACCATCCT GACCTACCCT TAATTCAGTC TAAGCAAAAT 
GAGGTGAATG CTGCCTGGGA GCGCCTTCGT GGTTTGGCTC TCCAGAGACA GAAAGCTCTG 
TCCAATGCTG CAAACTTACA ACGATTCAAA AGGGATGTGA CTGAAGCCAT CCAGTGGATC 
AAGGAGAAGG AACCTGTACT CACCTCTGAG GACTATGGCA AAGACCTTGT TGCCTCTGAA 
GGACTGTTTC ACAGTCACAA GGGACTTGAG AGAAATCTTG CTGTCATGAG TGACAAGGTG 
AAGGAGTTAT GTGCTAAAGC AGAGAAGCTG ACACTTTCCC ATCCTTCAGA TGCACCTCAG 
ATCCAGGAGA TGAAAGAAGA TCTGGTCTCC AGCTGGGAGC ATATTCGTGC CCTGGCCACC 
AGCAGATATG AAAAACTGCA GGCTACTTAT TGGTACCATC GATTTTCATC TGACTTTGAT 
GAACTCTCAG GCTGGATGAA CGAGAAGACT GCTGCGATCA ATGCTGATGA GCTGCCAACA 
GATGTGGCTG GTGGAGAAGT TCTGCTGGAC AGGCATCAGC AGCATAAGCA TGAGATTGAC 
TCTTACGATG ACCGATTTCA ATCTGCTGAT GAGACTGGTC AAGACCTCGT GAATGCCAAT 
CATGAAGCCT CTGATGAAGT TCGGGAAAAG ATGGAAATAC TTGACAACAA CTGGACTGCC 
CTGCTGGAAC TGTGGGACGA GCGTCATCGT CAGTATGAGC AGTGCTTGGA CTTTCATCTC 
TTCTACAGAG ACAGTGAGCA AGTGGACAGT TGGATGAGTA GACAAGAGGC CTTCCTGGAA 
AACGAGGATC TGGGAAACTC ACTGGGCAGT GCAGAAGCCC TTCTTCAGAA GCATGAAGAC 
TTTGAGGAAG CCTTTACTGC CCAGGAAGAG AAGATCATAA CTGTAGACAA GACTGCAACC 
AAATTGATTG GTGATGACCA TTATGATTCA GAGAACATCA AGGCTATCCG TGACGGGCTG 
TTAGCCCGGC GGGATGCCCT ACGTGAAAAG GCTGCCACTA GACGTAGATT GCTGAAGGAG 
TCATTGCTTC TGCAAAAACT GTATGAGGAC TCAGATGACC TAAAGAACTG GATCAACAAG 
AAGAAAAAGT TGGCAGATGA TGAAGATTAC AAGGACATAC AGAACTTGAA GAGCAGGGTT 
CAAAAGCAGC AAGTCTTTGA AAAGGAGTTG GCAGTTAATA AGACCCAGCT GGAAAACATA 
CAGAAAACTG GCCAAGAGAT GATTGAGGGT GGTCACTATG CCTCTGACAA TGTGACCACT 
CGTCTGAGTG AAGTTGCCAG CCTCTGGGAG GAGTTGCTGG AGGCTACAAA ACAGAAAGGG 
ACCCAGTTGC ATGAGGCCAA CCAGCAGCTG CAATTTGAAA ATAATGCAGA AGATTTGCAG 
CGCTGGCTGG AGGATGTTGA GTGGCAAGTC ACCTCTGAGG ATTATGGGAA AGGCCTGGCC 
GAGGTACAGA ATCGACTCAG GAAACACGGC CTCCTGGAGT CGGCTGTGGC TGCTCGTCAG 
GATCAGGTGG ATATCCTTAC AGACCTGGCT GCATATTTTG AAGAAATAGG CCATCCTGAT 
TCTAAGGATA TAAGGGCAAG GCAAGAGTCC TTGGTATGCC GATTTGAAGC TCTGAAAGAG 
CCACTGGCCA CCCGAAAGAA GAAGCTCTTA GACCTTCTCC ATCTGCAGCT GATTTGTAGA 
GACACAGAGG ATGAGGAGGC CTGGATCCAA GAGACTGAAC CCTCAGCTAC TTCCACCTAC 
CTTGGAAAGG ACCTGATTGC TTCCAAAAAG CTTCTGAATA GGCATAGAGT CATCCTGGAG 
AACATTGCCA GCCATGAACC ACGCATTCAA GAGATAACAG AAAGGGGAAA CAAAATGGTA 
GAGGAAGGAC ACTTTGCTGC AGAAGATGTG GCCTCTAGGG TCAAGAGTTT GAACCAGAAT 
ATGGAGTCTC TCCGTGCTCG AGCTGCTAGG CGACAAAATG ATCTTGAAGC CAATGTCCAG 
TTCCAGCAGT ACCTGGCTGA CCTGCATGAA GCAGAAACAT GGATCAGAGA GAAGGAACCT 
ATTGTAGATA ATACTAACTA TGGTGCTGAT GAAGAAGCAG CTGGGGCTCT TCTAAAGAAG 
CATGAGGCCT TTCTATTAGA TCTCAATTCA TTTGGAGACA GTATGAAAGC TCTGCGGAAT 
CAGGCAAACG CCTGCCAGCA ACAACAGGCT GCACCAGTGG AGGGAGTTGC TGGAGAACAA 
AGGGTCATGG CTTTATATGA CTTCCAGGCC CGCAGCCCCC GAGAAGTCAC CATGAAGAAA 
GGTGATGTCT TAACGCTGCT CAGTTCCATC AATAAGGACT GGTGGAAGGT GGAAGCTGCT 
GATCATCAGG GCATTGTCCC AGCTGTCTAT GTCAGAAGAC TGGCCCACGA TGAGTTCCCG 
ATGCTCCCAC AGCGGCGACG AGAAGAGCCA GGAAACATCA CCCAGCGCCA GGAGCAGATT 
GAGAACCAAT ACCGCTCCCT CTTGGATCGG GCAGAAGAAC GCAGACGTCG TCTATTGCAA 
CGTTATAATG AATTTTTATT GGCCTATGAG GCAGGAGACA TGCTGGAATG GATTCAAGAG 
AAAAAGGCAG AAAACACTGG AGTGGAACTA GATGATGTTT GGGAGCTGCA GAAAAAGTTT 
GATGAGTTCC AAAAGGATTT GAATACCAAT GAGCCTCGGC TAAGGGATAT CAACAAGGTA 
GCTGATGATC TACTATTTGA AGGACTTCTA ACACCAGAAG GAGCTCAAAT CCGGCAGGAA 
TTGAATTCCC GCTGGGGTTC TTTGCAGAGG CTTGCAGATG AACAGCGGCA GCTGCTGGGC 
AGTGCCCATG CTGTTGAAGT GTTTCACAGA GAAGCAGATG ACACGAAGGA GCAGATTGAG 
AAGAAATGCC AGGCCCTCAG TGCTGCAGAC CCTGGCTCAG ATCTGTTCAG TGTTCAGGCT 
CTTCAGCGAC GGCATGAGGG CTTTGAAAGG GACCTCGTAC CCCTGGGAGA TAAGGTGACC 
ATACTGGGGG AGACAGCAGA GCGGCTCAGT GAGTCCCATC CAGATGCCAC TGAGGACCTG 
CAGAGACAGA AAATGGAGCT GAATGAGGCC TGGGAAGACC TGCAGGGGCG TACAAAGGAT 
CGTAAGGAGA GCCTAAATGA GGCCCAGAAA TTCTACCTGT TCCTCAGCAA GGCCAGGGAT 
CTGCAGAACT GGATCAGTAG CATTGGTGGC ATGGTATCAT CACAGGAGCT GGCCGAAGAC 
TTAACTGGCA TAGAGATCTT GCTGGAGAGA CATCAGGAGC ACCGTGCTGA CATGGAGGCA 
GAGGCTCCCA CCTTCCAGGC CTTAGAGGAC TTCAGTGCAG AACTTATCGA CAGTGGGCAC 
CATGCTAGCC CTGAAATTGA AAAAAAGCTT CAAGCTGTCA AGCTAGAGAG AGATGATTTG 
GAGAAGGCTT GGGAAAAACG CAAGAAGATC CTAGACCAGT GCCTGGAGTT GCAGATGTTC 
CAGGGGAACT GTGATCAAGT TGAGAGCTGG ATGGTGGCAC GTGAGAATTC CCTGAGGTCA 
GATGACAAAA GTTCCTTAGA CAGTCTGGAG GCTTTGATGA AGAAACGGGA CGATTTGGAC 
AAAGCAATCA CTGCCCAGGA AGGGAAGATC ACTGACCTAG AACATTTTGC TGAGAGCCTC 
ATTGCTGATG AACACTATGC CAAAGAAGAG ATTGCTACGC GGCTCCAACG TGTACTAGAC 
AGGTGGAAGG CTCTCAAAGC ACAACTGATT GATGAGCGGA CAAAGCTTGG AGACTATGCC 
AACCTAAAAC AATTCTACCG AGACCTTGAG GAGCTGGAAG AATGGATCAG TGAGATGCTG 
CCCACAGCCT GTGATGAATC CTACAAAGAC GCCACTAACA TTCAGAGGAA ATACCTGAAA 
CACCAGACCT TTGCACATGA AGTCGATGGC CGATCTGAGC AGGTGCATGG CGTCATCAAC 
CTGGGGAACT CCCTGATTGA GTGTAGCGCT TGTGATGGCA ATGAAGAGGC CATGAAGGAG 
CAACTGGAAC AGCTGAAGGA ACATTGGGAT CATCTGCTTG AGAGAACAAA TGACAAAGGG 
AAGAAGCTCA ATGAGGCCAG TCGTCAACAG AGGTTCAACA CAAGCATCCG GGACTTTGAG 
TTCTGGCTCT CAGAGGCAGA GACATTGCTG GCCATGAAAG ATCAGGCCAG GGACTTGGCT 
TCAGCAGGAA ACCTACTCAA GAAGCATCAG CTATTGGAGA GAGAGATGTT GGCTCGAGAG 
GATGCACTCA AGGACCTGAA TACATTGGCT GAAGATTTGC TCTCCAGCGG GACTTTCAAC 
GTTGATCAGA TTGTGAAGAA AAAAGATAAT GTCAACAAGC GTTTCCTGAA TGTCCAAGAA 
TTGGCAGCTG CACACCACGA AAAATTGAAA GAGGCCTATG CCTTGTTCCA GTTCTTCCAG 
GATCTAGATG ATGAGGAATC CTGGATAGAG GAGAAGTTGA TACGAGTGAG CTCCCAGGAC 
TATGGGAGAG ATCTTCAGGG GGTTCAGAAC TTGCTGAAGA AGCACAAACG CCTAGAGGGG 
GAGCTGGTGG CCCATGAGCC TGCCATCCAG AATGTGCTGG ATATGGCAGA GAAGCTGAAA 
GACAAGGCTG CTGTGGGGCA AGAGGAGATC CAGTTGCGGC TGGCTCAGTT TGTTGAACAC 
TGGGAGAAGC TCAAAGAGTT GGCCAAGGCC CGAGGACTTA AGTTGGAAGA ATCCCTAGAA 
TACTTGCAAT TCATGCAGAA TGCTGAGGAA GAGGAAGCTT GGATCAATGA AAAGAATGCT 
TTGGCTGTCC GAGGAGATTG TGGAGATACA TTAGCTGCTA CTCAGAGCTT GCTAATGAAG 
CATGAAGCTT TGGAAAATGA CTTTGCTGTC CATGAGACCC GAGTACAAAA TGTGTGTGCA 
CAAGGAGAAG ACATCCTAAA TAAGGTGTTG CAGGAGGAAA GTCAGAACAA AGAGATTTCT 
TCCAAGATAG AGGCTCTGAA TGAAAAGACC CCTTCTCTGG CTAAGGCAAT AGCTGCTTGG 
AAGTTGCAAT TGGAAGACGA TTATGCCTTT CAGGAATTCA ACTGGAAGGC TGATGTGGTA 
GAGGCTTGGA TAGCTGATAA GGAAACAAGC CTAAAGACCA ATGGCAATGG TGCAGACCTT 
GGTGACTTCC TCACTCTTCT GGCAAAACAG GACACTCTGG ATGCCAGTCT GCAGAGTTTC 
CAGCAAGAGA GACTTCCCGA GATCACTGAC CTGAAGGACA AACTGATTTC TGCTCAACAC 
AACCAGTCTA AAGCCATTGA AGAGCGTTAT GCCGCTCTGC TGAAGCGCTG GGAACAGTTG 
CTGGAAGCCT CGGCAGTCCA CAGACAGAAA TTGCTGGAGA AACAGCTGCC TCTACAGAAG 
GTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPTA1
Entrez Gene ID	6708
Full Name	spectrin alpha, erythrocytic 1
Synonyms	EL2,HPP,HS3,SPH3,SPTA
General protein information	Preferred Names spectrin alpha, erythrocytic 1 Names spectrin alpha chain, erythrocytic 1 alpha-I spectrin elliptocytosis 2 erythroid alpha-spectrin spectrin alpha chain, erythrocyte
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q23.1
Summary	This gene encodes a member of a family of molecular scaffold proteins that link the plasma membrane to the actin cytoskeleton and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. The encoded protein is primarily composed of 22 spectrin repeats which are involved in dimer formation. It forms a component of the erythrocyte plasma membrane. Mutations in this gene result in a variety of hereditary red blood cell disorders, including elliptocytosis-2, pyropoikilocytosis, and spherocytosis, type 3. [provided by RefSeq, Aug 2017].
Disorder MIM:	182860
Disorder Html:	Elliptocytosis-2, 130600 (3); Pyropoikilocytosis, 266140 (3); Spherocytosis, type 3, 270970 (3)

Homo sapiens (human)	SPTA1	NP_003117.2
	SPTA1	XP_001117115.1
Bos taurus (cattle)	SPTA1	NP_001193517.1
Mus musculus (house mouse)	Spta1	NP_035595.2
Pan troglodytes (chimpanzee)	SPTA1	XP_001169940.1
Canis lupus familiaris (dog)	SPTA1	XP_545733.3
Rattus norvegicus (Norway rat)	Spta1	NP_001011908.2

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPTA1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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