SPRR3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPRR3
Entrez Gene ID	6707
Full Name	small proline rich protein 3
General protein information	Preferred Names small proline rich protein 3 Names small proline-rich protein 3 22 kDa pancornulin cornifin beta esophagin
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q21.3
Disorder MIM:	182271

mRNA and Protein(s)

mRNA	Protein	Name
NM_001097589.1	NP_001091058.1	small proline-rich protein 3
NM_005416.2	NP_005407.1	small proline-rich protein 3
NM_001097589.2	NP_001091058.1	small proline-rich protein 3

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Sprr3	NP_001101156.1
Mus musculus (house mouse)	Sprr3	NP_035608.1
Homo sapiens (human)	SPRR3	NP_005407.1
Canis lupus familiaris (dog)	LOC100685649	XP_005630895.1
Pan troglodytes (chimpanzee)	SPRR3	XP_003949601.1
	LOC713857	XP_001109921.1

Gene Ontology
Bibliography

Related articles in PubMed

Expression of Cornified Envelope Proteins in Skin and Its Relationship with Atopic Dermatitis Phenotype.
Trzeciak M, Sakowicz-Burkiewicz M, Wesserling M, Dobaczewska D, Gle? J, Nowicki R, Pawelczyk T
Acta dermato-venereologica97(1)36-41(2017 01)

Small proline-rich repeat protein 3 enhances the sensitivity of esophageal cancer cells in response to DNA damage-induced apoptosis.
Luo A, Chen H, Ding F, Zhang Y, Wang M, Xiao Z, Liu Z
Molecular oncology7(5)955-67(2013 Oct)

Expression of SPRR3 is associated with tumor cell proliferation in less advanced stages of breast cancer.
Kim JC, Yu JH, Cho YK, Jung CS, Ahn SH, Gong G, Kim YS, Cho DH
Breast cancer research and treatment133(3)909-16(2012 Jun)

Quantitative evaluation of SPRR3 expression in esophageal squamous cell carcinoma by qPCR and its potential use as a biomarker.
de A Sim?o T, Souza-Santos PT, de Oliveira DS, Bernardo V, Lima SC, Rapozo DC, Kruel CD, Faria PA, Ribeiro Pinto LF, Albano RM
Experimental and molecular pathology91(2)584-9(2011 Oct)

Association screening in the Epidermal Differentiation Complex (EDC) identifies an SPRR3 repeat number variant as a risk factor for eczema.
Marenholz I, Rivera VA, Esparza-Gordillo J, Bauerfeind A, Lee-Kirsch MA, Ciechanowicz A, Kurek M, Piskackova T, Macek M, Lee YA
The Journal of investigative dermatology131(8)1644-9(2011 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SPRR3v2 mRNA level in non-lesional AD skin correlated with severity of AD, and SPRR3 protein level in non-lesional AD skin correlated inversely with pruritus.
Title: Expression of Cornified Envelope Proteins in Skin and Its Relationship with Atopic Dermatitis Phenotype.

Results implicate that SPRR3 might serve as a radiation-sensitive predictor of ESCC.
Title: Small proline-rich repeat protein 3 enhances the sensitivity of esophageal cancer cells in response to DNA damage-induced apoptosis.

This study demonstrates that SPRR3 promotes BC cell proliferation by enhancing p53 degradation via the AKT and MAPK pathways
Title: Expression of SPRR3 is associated with tumor cell proliferation in less advanced stages of breast cancer.

The quantitative analysis of SPRR3 mRNA is a robust and reliable method to monitor the malignant transformation of the healthy esophageal mucosa into esophageal squamous cell carcinoma.
Title: Quantitative evaluation of SPRR3 expression in esophageal squamous cell carcinoma by qPCR and its potential use as a biomarker.

The SPRR3 variant associated with eczema carried an extra 24-bp repeat in the central domain, which may alter the physical properties of the cornified cell envelope
Title: Association screening in the Epidermal Differentiation Complex (EDC) identifies an SPRR3 repeat number variant as a risk factor for eczema.

The following SPRR3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPRR3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17324	NM_001097589.2 Latest version!	Homo sapiens small proline rich protein 3 (SPRR3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu17324	NM_005416.2 Latest version!	Homo sapiens small proline rich protein 3 (SPRR3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu17324	NM_001097589.1	Homo sapiens small proline rich protein 3 (SPRR3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPRR3 ( NM_001097589.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091058.1 Homo sapiens small proline rich protein 3 (SPRR3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17324
Clone ID Related Accession (Same CDS sequence)	NM_001097589.1 , NM_005416.2 , NM_001097589.2
Accession Version	NM_001097589.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	small proline-rich protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA451130.1, DA763011.1, AL356867.23 and BM955458.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279835.110.1, ERR279855.2864.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTCTT ACCAGCAGAA GCAGACCTTT ACCCCACCAC CTCAGCTTCA ACAGCAGCAG 
GTGAAACAAC CCAGCCAGCC TCCACCTCAG GAAATATTTG TTCCCACAAC CAAGGAGCCA 
TGCCACTCAA AGGTTCCACA ACCTGGAAAC ACAAAGATTC CAGAGCCAGG CTGTACCAAG 
GTCCCTGAGC CAGGCTGTAC CAAGGTCCCT GAGCCAGGCT GTACCAAGGT CCCTGAGCCA 
GGTTGTACCA AGGTCCCTGA GCCAGGCTGT ACCAAGGTCC CTGAGCCAGG TTGTACCAAG 
GTCCCTGAGC CAGGCTACAC CAAGGTCCCT GAACCAGGCA GCATCAAGGT CCCTGACCAA 
GGCTTCATCA AGTTTCCTGA GCCAGGTGCC ATCAAAGTTC CTGAGCAAGG ATACACCAAA 
GTTCCTGTGC CAGGCTACAC AAAGCTACCA GAGCCATGTC CTTCAACGGT CACTCCAGGC 
CCAGCTCAGC AGAAGACCAA GCAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091058.1
CDS	67..576
Translation	MSSYQQKQTFTPPPQLQQQQVKQPSQPPPQEIFVPTTKEPCHSKVPQPGNTKIPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGYTKVPEPGSIKVPDQGFIKFPEPGAIKVPEQGYTKVPVPGYTKLPEPCPSTVTPGPAQQKTKQK

Target ORF information:

RefSeq Version	NM_001097589.1
Organism	Homo sapiens(human)
Definition	Homo sapiens small proline rich protein 3 (SPRR3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097589.1

ORF Insert Sequence:

ATGAGTTCTT ACCAGCAGAA GCAGACCTTT ACCCCACCAC CTCAGCTTCA ACAGCAGCAG 
GTGAAACAAC CCAGCCAGCC TCCACCTCAG GAAATATTTG TTCCCACAAC CAAGGAGCCA 
TGCCACTCAA AGGTTCCACA ACCTGGAAAC ACAAAGATTC CAGAGCCAGG CTGTACCAAG 
GTCCCTGAGC CAGGCTGTAC CAAGGTCCCT GAGCCAGGCT GTACCAAGGT CCCTGAGCCA 
GGTTGTACCA AGGTCCCTGA GCCAGGCTGT ACCAAGGTCC CTGAGCCAGG TTGTACCAAG 
GTCCCTGAGC CAGGCTACAC CAAGGTCCCT GAACCAGGCA GCATCAAGGT CCCTGACCAA 
GGCTTCATCA AGTTTCCTGA GCCAGGTGCC ATCAAAGTTC CTGAGCAAGG ATACACCAAA 
GTTCCTGTGC CAGGCTACAC AAAGCTACCA GAGCCATGTC CTTCAACGGT CACTCCAGGC 
CCAGCTCAGC AGAAGACCAA GCAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPRR3 ( NM_005416.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_005407.1 Homo sapiens small proline rich protein 3 (SPRR3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu17324
Clone ID Related Accession (Same CDS sequence)	NM_001097589.1 , NM_005416.2 , NM_001097589.2
Accession Version	NM_005416.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Homo sapiens(human)
Product	small proline-rich protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA451130.1, BF826427.1, AL356867.23 and BM955458.1. On May 22, 2007 this sequence version replaced NM_005416.1. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: ERR279830.1690.1, ERR279875.5344.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2144333, SAMEA2147975 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTTCTT ACCAGCAGAA GCAGACCTTT ACCCCACCAC CTCAGCTTCA ACAGCAGCAG 
GTGAAACAAC CCAGCCAGCC TCCACCTCAG GAAATATTTG TTCCCACAAC CAAGGAGCCA 
TGCCACTCAA AGGTTCCACA ACCTGGAAAC ACAAAGATTC CAGAGCCAGG CTGTACCAAG 
GTCCCTGAGC CAGGCTGTAC CAAGGTCCCT GAGCCAGGCT GTACCAAGGT CCCTGAGCCA 
GGTTGTACCA AGGTCCCTGA GCCAGGCTGT ACCAAGGTCC CTGAGCCAGG TTGTACCAAG 
GTCCCTGAGC CAGGCTACAC CAAGGTCCCT GAACCAGGCA GCATCAAGGT CCCTGACCAA 
GGCTTCATCA AGTTTCCTGA GCCAGGTGCC ATCAAAGTTC CTGAGCAAGG ATACACCAAA 
GTTCCTGTGC CAGGCTACAC AAAGCTACCA GAGCCATGTC CTTCAACGGT CACTCCAGGC 
CCAGCTCAGC AGAAGACCAA GCAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005407.1
CDS	151..660
Translation	MSSYQQKQTFTPPPQLQQQQVKQPSQPPPQEIFVPTTKEPCHSKVPQPGNTKIPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGYTKVPEPGSIKVPDQGFIKFPEPGAIKVPEQGYTKVPVPGYTKLPEPCPSTVTPGPAQQKTKQK

Target ORF information:

RefSeq Version	NM_005416.2
Organism	Homo sapiens(human)
Definition	Homo sapiens small proline rich protein 3 (SPRR3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005416.2

ORF Insert Sequence:

ATGAGTTCTT ACCAGCAGAA GCAGACCTTT ACCCCACCAC CTCAGCTTCA ACAGCAGCAG 
GTGAAACAAC CCAGCCAGCC TCCACCTCAG GAAATATTTG TTCCCACAAC CAAGGAGCCA 
TGCCACTCAA AGGTTCCACA ACCTGGAAAC ACAAAGATTC CAGAGCCAGG CTGTACCAAG 
GTCCCTGAGC CAGGCTGTAC CAAGGTCCCT GAGCCAGGCT GTACCAAGGT CCCTGAGCCA 
GGTTGTACCA AGGTCCCTGA GCCAGGCTGT ACCAAGGTCC CTGAGCCAGG TTGTACCAAG 
GTCCCTGAGC CAGGCTACAC CAAGGTCCCT GAACCAGGCA GCATCAAGGT CCCTGACCAA 
GGCTTCATCA AGTTTCCTGA GCCAGGTGCC ATCAAAGTTC CTGAGCAAGG ATACACCAAA 
GTTCCTGTGC CAGGCTACAC AAAGCTACCA GAGCCATGTC CTTCAACGGT CACTCCAGGC 
CCAGCTCAGC AGAAGACCAA GCAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPRR3 ( NM_001097589.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001091058.1 Homo sapiens small proline rich protein 3 (SPRR3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu17324
Clone ID Related Accession (Same CDS sequence)	NM_001097589.1 , NM_005416.2 , NM_001097589.2
Accession Version	NM_001097589.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 510bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-23
Organism	Homo sapiens(human)
Product	small proline-rich protein 3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA763011.1, AL356867.23 and BM955458.1. On Nov 22, 2018 this sequence version replaced NM_001097589.1. Transcript Variant: This variant (2) lacks an alternate exon in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017802.1, ERR279875.6200.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2144335 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000295367.5/ ENSP00000295367.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGAGTTCTT ACCAGCAGAA GCAGACCTTT ACCCCACCAC CTCAGCTTCA ACAGCAGCAG 
GTGAAACAAC CCAGCCAGCC TCCACCTCAG GAAATATTTG TTCCCACAAC CAAGGAGCCA 
TGCCACTCAA AGGTTCCACA ACCTGGAAAC ACAAAGATTC CAGAGCCAGG CTGTACCAAG 
GTCCCTGAGC CAGGCTGTAC CAAGGTCCCT GAGCCAGGCT GTACCAAGGT CCCTGAGCCA 
GGTTGTACCA AGGTCCCTGA GCCAGGCTGT ACCAAGGTCC CTGAGCCAGG TTGTACCAAG 
GTCCCTGAGC CAGGCTACAC CAAGGTCCCT GAACCAGGCA GCATCAAGGT CCCTGACCAA 
GGCTTCATCA AGTTTCCTGA GCCAGGTGCC ATCAAAGTTC CTGAGCAAGG ATACACCAAA 
GTTCCTGTGC CAGGCTACAC AAAGCTACCA GAGCCATGTC CTTCAACGGT CACTCCAGGC 
CCAGCTCAGC AGAAGACCAA GCAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001091058.1
CDS	65..574
Translation	MSSYQQKQTFTPPPQLQQQQVKQPSQPPPQEIFVPTTKEPCHSKVPQPGNTKIPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGCTKVPEPGYTKVPEPGSIKVPDQGFIKFPEPGAIKVPEQGYTKVPVPGYTKLPEPCPSTVTPGPAQQKTKQK

Target ORF information:

RefSeq Version	NM_001097589.2
Organism	Homo sapiens(human)
Definition	Homo sapiens small proline rich protein 3 (SPRR3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001097589.2

ORF Insert Sequence:

ATGAGTTCTT ACCAGCAGAA GCAGACCTTT ACCCCACCAC CTCAGCTTCA ACAGCAGCAG 
GTGAAACAAC CCAGCCAGCC TCCACCTCAG GAAATATTTG TTCCCACAAC CAAGGAGCCA 
TGCCACTCAA AGGTTCCACA ACCTGGAAAC ACAAAGATTC CAGAGCCAGG CTGTACCAAG 
GTCCCTGAGC CAGGCTGTAC CAAGGTCCCT GAGCCAGGCT GTACCAAGGT CCCTGAGCCA 
GGTTGTACCA AGGTCCCTGA GCCAGGCTGT ACCAAGGTCC CTGAGCCAGG TTGTACCAAG 
GTCCCTGAGC CAGGCTACAC CAAGGTCCCT GAACCAGGCA GCATCAAGGT CCCTGACCAA 
GGCTTCATCA AGTTTCCTGA GCCAGGTGCC ATCAAAGTTC CTGAGCAAGG ATACACCAAA 
GTTCCTGTGC CAGGCTACAC AAAGCTACCA GAGCCATGTC CTTCAACGGT CACTCCAGGC 
CCAGCTCAGC AGAAGACCAA GCAGAAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Expression of Cornified Envelope Proteins in Skin and Its Relationship with Atopic Dermatitis Phenotype.
Acta dermato-venereologica97(1)36-41(2017 01)
Trzeciak M,Sakowicz-Burkiewicz M,Wesserling M,Dobaczewska D,Gle? J,Nowicki R,Pawelczyk T

Small proline-rich repeat protein 3 enhances the sensitivity of esophageal cancer cells in response to DNA damage-induced apoptosis.
Molecular oncology7(5)955-67(2013 Oct)
Luo A,Chen H,Ding F,Zhang Y,Wang M,Xiao Z,Liu Z

Expression of SPRR3 is associated with tumor cell proliferation in less advanced stages of breast cancer.
Breast cancer research and treatment133(3)909-16(2012 Jun)
Kim JC,Yu JH,Cho YK,Jung CS,Ahn SH,Gong G,Kim YS,Cho DH

Quantitative evaluation of SPRR3 expression in esophageal squamous cell carcinoma by qPCR and its potential use as a biomarker.
Experimental and molecular pathology91(2)584-9(2011 Oct)
de A Sim?o T,Souza-Santos PT,de Oliveira DS,Bernardo V,Lima SC,Rapozo DC,Kruel CD,Faria PA,Ribeiro Pinto LF,Albano RM

Association screening in the Epidermal Differentiation Complex (EDC) identifies an SPRR3 repeat number variant as a risk factor for eczema.
The Journal of investigative dermatology131(8)1644-9(2011 Aug)
Marenholz I,Rivera VA,Esparza-Gordillo J,Bauerfeind A,Lee-Kirsch MA,Ciechanowicz A,Kurek M,Piskackova T,Macek M,Lee YA

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SPRR3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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