SPAST gene cDNA ORF clone, Homo sapiens(human)

mRNA	Protein	Name
NM_001363875.1	NP_001350804.1	spastin isoform 4
XM_017004778.1	XP_016860267.1	spastin isoform X4
NM_199436.1	NP_955468.1	spastin isoform 2
XM_005264516.4	XP_005264573.1	spastin isoform X1
XM_017004778.2	XP_016860267.1	spastin isoform X4
XM_017004777.1	XP_016860266.1	spastin isoform X2
NM_014946.3	NP_055761.2	spastin isoform 1
NM_001363823.1	NP_001350752.1	spastin isoform 3
XM_005264516.5	XP_005264573.1	spastin isoform X1
XM_011533067.2	XP_011531369.1	spastin isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu25324	NM_014946.3 Latest version!	Homo sapiens spastin (SPAST), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu25537	NM_199436.1 Latest version!	Homo sapiens spastin (SPAST), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu35573	NM_001363823.1 Latest version!	Homo sapiens spastin (SPAST), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu89825	NM_001363875.1 Latest version!	Homo sapiens spastin (SPAST), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu35573	XM_005264516.5 Latest version!	Homo sapiens spastin (SPAST), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu60708	XM_011533067.2 Latest version!	Homo sapiens spastin (SPAST), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu89825	XM_017004777.1 Latest version!	Homo sapiens spastin (SPAST), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu89826	XM_017004778.2 Latest version!	Homo sapiens spastin (SPAST), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
View Old Accession Versions >>
OHu89826	XM_017004778.1	Homo sapiens spastin (SPAST), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
OHu35573	XM_005264516.4	Homo sapiens spastin (SPAST), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$307.30 $439.00
Hide Old Accession Versions >>

SPAST ( NM_014946.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_055761.2 Homo sapiens spastin (SPAST), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu25324
Clone ID Related Accession (Same CDS sequence)	NM_014946.3
Accession Version	NM_014946.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	spastin isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB029006.1, AJ246001.1, BG532310.1 and AA830062.1. This sequence is a reference standard in the RefSeqGene project. On Jan 13, 2004 this sequence version replaced NM_014946.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1; also known as M1). It also encodes a shorter isoform (M87) from an alternate, in-frame, downstream translation initiation site. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.105119.1, SRR1803614.230472.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA 
CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA 
GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA 
ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAGAA GATGCAACCA 
GTTTTGCCAT TTTCCAAGTC ACAAACGGAC GTCTATAATG ACAGTACTAA CTTGGCATGC 
CGCAATGGAC ATCTCCAGTC AGAAAGTGGA GCTGTTCCAA AAAGAAAAGA CCCCTTAACA 
CACACTAGTA ATTCACTGCC TCGTTCAAAA ACAGTTATGA AAACTGGATC TGCAGGCCTT 
TCAGGCCACC ATAGAGCACC TAGTTACAGT GGTTTATCCA TGGTTTCTGG AGTGAAACAG 
GGATCTGGTC CTGCTCCTAC CACTCATAAG GGTACTCCGA AAACAAATAG GACAAATAAA 
CCTTCTACCC CTACAACTGC TACTCGTAAG AAAAAAGACT TGAAGAATTT TAGGAATGTG 
GACAGCAACC TTGCTAACCT TATAATGAAT GAAATTGTGG ACAATGGAAC AGCTGTTAAA 
TTTGATGATA TAGCTGGTCA AGACTTGGCA AAACAAGCAT TGCAAGAAAT TGTTATTCTT 
CCTTCTCTGA GGCCTGAGTT GTTCACAGGG CTTAGAGCTC CTGCCAGAGG GCTGTTACTC 
TTTGGTCCAC CTGGGAATGG GAAGACAATG CTGGCTAAAG CAGTAGCTGC AGAATCGAAT 
GCAACCTTCT TTAATATAAG TGCTGCAAGT TTAACTTCAA AATACGTGGG AGAAGGAGAG 
AAATTGGTGA GGGCTCTTTT TGCTGTGGCT CGAGAACTTC AACCTTCTAT AATTTTTATA 
GATGAAGTTG ATAGCCTTTT GTGTGAAAGA AGAGAAGGGG AGCACGATGC TAGTAGACGC 
CTAAAAACTG AATTTCTAAT AGAATTTGAT GGTGTACAGT CTGCTGGAGA TGACAGAGTA 
CTTGTAATGG GTGCAACTAA TAGGCCACAA GAGCTTGATG AGGCTGTTCT CAGGCGTTTC 
ATCAAACGGG TATATGTGTC TTTACCAAAT GAGGAGACAA GACTACTTTT GCTTAAAAAT 
CTGTTATGTA AACAAGGAAG TCCATTGACC CAAAAAGAAC TAGCACAACT TGCTAGAATG 
ACTGATGGAT ACTCAGGAAG TGACCTAACA GCTTTGGCAA AAGATGCAGC ACTGGGTCCT 
ATCCGAGAAC TAAAACCAGA ACAGGTGAAG AATATGTCTG CCAGTGAGAT GAGAAATATT 
CGATTATCTG ACTTCACTGA ATCCTTGAAA AAAATAAAAC GCAGCGTCAG CCCTCAAACT 
TTAGAAGCGT ACATACGTTG GAACAAGGAC TTTGGAGATA CCACTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_055761.2
CDS	222..2072
Translation	MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLEKMQPVLPFSKSQTDVYNDSTNLACRNGHLQSESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	NM_014946.3
Organism	Homo sapiens(human)
Definition	Homo sapiens spastin (SPAST), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_014946.3

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA 
CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA 
GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA 
ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAGAA GATGCAACCA 
GTTTTGCCAT TTTCCAAGTC ACAAACGGAC GTCTATAATG ACAGTACTAA CTTGGCATGC 
CGCAATGGAC ATCTCCAGTC AGAAAGTGGA GCTGTTCCAA AAAGAAAAGA CCCCTTAACA 
CACACTAGTA ATTCACTGCC TCGTTCAAAA ACAGTTATGA AAACTGGATC TGCAGGCCTT 
TCAGGCCACC ATAGAGCACC TAGTTACAGT GGTTTATCCA TGGTTTCTGG AGTGAAACAG 
GGATCTGGTC CTGCTCCTAC CACTCATAAG GGTACTCCGA AAACAAATAG GACAAATAAA 
CCTTCTACCC CTACAACTGC TACTCGTAAG AAAAAAGACT TGAAGAATTT TAGGAATGTG 
GACAGCAACC TTGCTAACCT TATAATGAAT GAAATTGTGG ACAATGGAAC AGCTGTTAAA 
TTTGATGATA TAGCTGGTCA AGACTTGGCA AAACAAGCAT TGCAAGAAAT TGTTATTCTT 
CCTTCTCTGA GGCCTGAGTT GTTCACAGGG CTTAGAGCTC CTGCCAGAGG GCTGTTACTC 
TTTGGTCCAC CTGGGAATGG GAAGACAATG CTGGCTAAAG CAGTAGCTGC AGAATCGAAT 
GCAACCTTCT TTAATATAAG TGCTGCAAGT TTAACTTCAA AATACGTGGG AGAAGGAGAG 
AAATTGGTGA GGGCTCTTTT TGCTGTGGCT CGAGAACTTC AACCTTCTAT AATTTTTATA 
GATGAAGTTG ATAGCCTTTT GTGTGAAAGA AGAGAAGGGG AGCACGATGC TAGTAGACGC 
CTAAAAACTG AATTTCTAAT AGAATTTGAT GGTGTACAGT CTGCTGGAGA TGACAGAGTA 
CTTGTAATGG GTGCAACTAA TAGGCCACAA GAGCTTGATG AGGCTGTTCT CAGGCGTTTC 
ATCAAACGGG TATATGTGTC TTTACCAAAT GAGGAGACAA GACTACTTTT GCTTAAAAAT 
CTGTTATGTA AACAAGGAAG TCCATTGACC CAAAAAGAAC TAGCACAACT TGCTAGAATG 
ACTGATGGAT ACTCAGGAAG TGACCTAACA GCTTTGGCAA AAGATGCAGC ACTGGGTCCT 
ATCCGAGAAC TAAAACCAGA ACAGGTGAAG AATATGTCTG CCAGTGAGAT GAGAAATATT 
CGATTATCTG ACTTCACTGA ATCCTTGAAA AAAATAAAAC GCAGCGTCAG CCCTCAAACT 
TTAGAAGCGT ACATACGTTG GAACAAGGAC TTTGGAGATA CCACTGTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( NM_199436.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_955468.1 Homo sapiens spastin (SPAST), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu25537
Clone ID Related Accession (Same CDS sequence)	NM_199436.1
Accession Version	NM_199436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1755bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	spastin isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB029006.1, BG532310.1 and AA830062.1. Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (2), compared to isoform 1. This variant is also predicted to use an alternate, in-frame, downstream translation initiation site to encode an even shorter isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150260.1, SRR1660805.241629.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA 
CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA 
GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA 
ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAAAG TGGAGCTGTT 
CCAAAAAGAA AAGACCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT 
ATGAAAACTG GATCTGCAGG CCTTTCAGGC CACCATAGAG CACCTAGTTA CAGTGGTTTA 
TCCATGGTTT CTGGAGTGAA ACAGGGATCT GGTCCTGCTC CTACCACTCA TAAGGGTACT 
CCGAAAACAA ATAGGACAAA TAAACCTTCT ACCCCTACAA CTGCTACTCG TAAGAAAAAA 
GACTTGAAGA ATTTTAGGAA TGTGGACAGC AACCTTGCTA ACCTTATAAT GAATGAAATT 
GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GTCAAGACTT GGCAAAACAA 
GCATTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA 
GCTCCTGCCA GAGGGCTGTT ACTCTTTGGT CCACCTGGGA ATGGGAAGAC AATGCTGGCT 
AAAGCAGTAG CTGCAGAATC GAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT 
TCAAAATACG TGGGAGAAGG AGAGAAATTG GTGAGGGCTC TTTTTGCTGT GGCTCGAGAA 
CTTCAACCTT CTATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA 
GGGGAGCACG ATGCTAGTAG ACGCCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA 
CAGTCTGCTG GAGATGACAG AGTACTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT 
GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTACC AAATGAGGAG 
ACAAGACTAC TTTTGCTTAA AAATCTGTTA TGTAAACAAG GAAGTCCATT GACCCAAAAA 
GAACTAGCAC AACTTGCTAG AATGACTGAT GGATACTCAG GAAGTGACCT AACAGCTTTG 
GCAAAAGATG CAGCACTGGG TCCTATCCGA GAACTAAAAC CAGAACAGGT GAAGAATATG 
TCTGCCAGTG AGATGAGAAA TATTCGATTA TCTGACTTCA CTGAATCCTT GAAAAAAATA 
AAACGCAGCG TCAGCCCTCA AACTTTAGAA GCGTACATAC GTTGGAACAA GGACTTTGGA 
GATACCACTG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955468.1
CDS	222..1976
Translation	MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	NM_199436.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spastin (SPAST), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199436.1

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA 
CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA 
GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA 
ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAAAG TGGAGCTGTT 
CCAAAAAGAA AAGACCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT 
ATGAAAACTG GATCTGCAGG CCTTTCAGGC CACCATAGAG CACCTAGTTA CAGTGGTTTA 
TCCATGGTTT CTGGAGTGAA ACAGGGATCT GGTCCTGCTC CTACCACTCA TAAGGGTACT 
CCGAAAACAA ATAGGACAAA TAAACCTTCT ACCCCTACAA CTGCTACTCG TAAGAAAAAA 
GACTTGAAGA ATTTTAGGAA TGTGGACAGC AACCTTGCTA ACCTTATAAT GAATGAAATT 
GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GTCAAGACTT GGCAAAACAA 
GCATTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA 
GCTCCTGCCA GAGGGCTGTT ACTCTTTGGT CCACCTGGGA ATGGGAAGAC AATGCTGGCT 
AAAGCAGTAG CTGCAGAATC GAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT 
TCAAAATACG TGGGAGAAGG AGAGAAATTG GTGAGGGCTC TTTTTGCTGT GGCTCGAGAA 
CTTCAACCTT CTATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA 
GGGGAGCACG ATGCTAGTAG ACGCCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA 
CAGTCTGCTG GAGATGACAG AGTACTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT 
GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTACC AAATGAGGAG 
ACAAGACTAC TTTTGCTTAA AAATCTGTTA TGTAAACAAG GAAGTCCATT GACCCAAAAA 
GAACTAGCAC AACTTGCTAG AATGACTGAT GGATACTCAG GAAGTGACCT AACAGCTTTG 
GCAAAAGATG CAGCACTGGG TCCTATCCGA GAACTAAAAC CAGAACAGGT GAAGAATATG 
TCTGCCAGTG AGATGAGAAA TATTCGATTA TCTGACTTCA CTGAATCCTT GAAAAAAATA 
AAACGCAGCG TCAGCCCTCA AACTTTAGAA GCGTACATAC GTTGGAACAA GGACTTTGGA 
GATACCACTG TTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( NM_001363823.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350752.1 Homo sapiens spastin (SPAST), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu35573
Clone ID Related Accession (Same CDS sequence)	XM_005264516.4 , NM_001363823.1 , XM_005264516.5
Accession Version	NM_001363823.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	spastin isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121655.1 and AL121658.4. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, which results in a shorter isoform (3), compared to isoform 1. This variant is also predicted to use an alternate, in-frame, downstream translation initiation site to encode an even shorter isoform. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG 
AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT 
GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG 
ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAGAAGAT GCAACCAGTT 
TTGCCATTTT CCAAGTCACA AACGGACGTC TATAATGACA GTACTAACTT GGCATGCCGC 
AATGGACATC TCCAGTCAGA AAGTGGAGCT GTTCCAAAAA GAAAAGACCC CTTAACACAC 
ACTAGTAATT CACTGCCTCG TTCAAAAACA GTTATGAAAA CTGGATCTGC AGGCCTTTCA 
GGCCACCATA GAGCACCTAG TTACAGTGGT TTATCCATGG TTTCTGGAGT GAAACAGGGA 
TCTGGTCCTG CTCCTACCAC TCATAAGGGT ACTCCGAAAA CAAATAGGAC AAATAAACCT 
TCTACCCCTA CAACTGCTAC TCGTAAGAAA AAAGACTTGA AGAATTTTAG GAATGTGGAC 
AGCAACCTTG CTAACCTTAT AATGAATGAA ATTGTGGACA ATGGAACAGC TGTTAAATTT 
GATGATATAG CTGGTCAAGA CTTGGCAAAA CAAGCATTGC AAGAAATTGT TATTCTTCCT 
TCTCTGAGGC CTGAGTTGTT CACAGGGCTT AGAGCTCCTG CCAGAGGGCT GTTACTCTTT 
GGTCCACCTG GGAATGGGAA GACAATGCTG GCTAAAGCAG TAGCTGCAGA ATCGAATGCA 
ACCTTCTTTA ATATAAGTGC TGCAAGTTTA ACTTCAAAAT ACGTGGGAGA AGGAGAGAAA 
TTGGTGAGGG CTCTTTTTGC TGTGGCTCGA GAACTTCAAC CTTCTATAAT TTTTATAGAT 
GAAGTTGATA GCCTTTTGTG TGAAAGAAGA GAAGGGGAGC ACGATGCTAG TAGACGCCTA 
AAAACTGAAT TTCTAATAGA ATTTGATGGT GTACAGTCTG CTGGAGATGA CAGAGTACTT 
GTAATGGGTG CAACTAATAG GCCACAAGAG CTTGATGAGG CTGTTCTCAG GCGTTTCATC 
AAACGGGTAT ATGTGTCTTT ACCAAATGAG GAGACAAGAC TACTTTTGCT TAAAAATCTG 
TTATGTAAAC AAGGAAGTCC ATTGACCCAA AAAGAACTAG CACAACTTGC TAGAATGACT 
GATGGATACT CAGGAAGTGA CCTAACAGCT TTGGCAAAAG ATGCAGCACT GGGTCCTATC 
CGAGAACTAA AACCAGAACA GGTGAAGAAT ATGTCTGCCA GTGAGATGAG AAATATTCGA 
TTATCTGACT TCACTGAATC CTTGAAAAAA ATAAAACGCA GCGTCAGCCC TCAAACTTTA 
GAAGCGTACA TACGTTGGAA CAAGGACTTT GGAGATACCA CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350752.1
CDS	222..2069
Translation	MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLEKMQPVLPFSKSQTDVYNDSTNLACRNGHLQSESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	NM_001363823.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spastin (SPAST), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363823.1

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG 
AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT 
GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG 
ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAGAAGAT GCAACCAGTT 
TTGCCATTTT CCAAGTCACA AACGGACGTC TATAATGACA GTACTAACTT GGCATGCCGC 
AATGGACATC TCCAGTCAGA AAGTGGAGCT GTTCCAAAAA GAAAAGACCC CTTAACACAC 
ACTAGTAATT CACTGCCTCG TTCAAAAACA GTTATGAAAA CTGGATCTGC AGGCCTTTCA 
GGCCACCATA GAGCACCTAG TTACAGTGGT TTATCCATGG TTTCTGGAGT GAAACAGGGA 
TCTGGTCCTG CTCCTACCAC TCATAAGGGT ACTCCGAAAA CAAATAGGAC AAATAAACCT 
TCTACCCCTA CAACTGCTAC TCGTAAGAAA AAAGACTTGA AGAATTTTAG GAATGTGGAC 
AGCAACCTTG CTAACCTTAT AATGAATGAA ATTGTGGACA ATGGAACAGC TGTTAAATTT 
GATGATATAG CTGGTCAAGA CTTGGCAAAA CAAGCATTGC AAGAAATTGT TATTCTTCCT 
TCTCTGAGGC CTGAGTTGTT CACAGGGCTT AGAGCTCCTG CCAGAGGGCT GTTACTCTTT 
GGTCCACCTG GGAATGGGAA GACAATGCTG GCTAAAGCAG TAGCTGCAGA ATCGAATGCA 
ACCTTCTTTA ATATAAGTGC TGCAAGTTTA ACTTCAAAAT ACGTGGGAGA AGGAGAGAAA 
TTGGTGAGGG CTCTTTTTGC TGTGGCTCGA GAACTTCAAC CTTCTATAAT TTTTATAGAT 
GAAGTTGATA GCCTTTTGTG TGAAAGAAGA GAAGGGGAGC ACGATGCTAG TAGACGCCTA 
AAAACTGAAT TTCTAATAGA ATTTGATGGT GTACAGTCTG CTGGAGATGA CAGAGTACTT 
GTAATGGGTG CAACTAATAG GCCACAAGAG CTTGATGAGG CTGTTCTCAG GCGTTTCATC 
AAACGGGTAT ATGTGTCTTT ACCAAATGAG GAGACAAGAC TACTTTTGCT TAAAAATCTG 
TTATGTAAAC AAGGAAGTCC ATTGACCCAA AAAGAACTAG CACAACTTGC TAGAATGACT 
GATGGATACT CAGGAAGTGA CCTAACAGCT TTGGCAAAAG ATGCAGCACT GGGTCCTATC 
CGAGAACTAA AACCAGAACA GGTGAAGAAT ATGTCTGCCA GTGAGATGAG AAATATTCGA 
TTATCTGACT TCACTGAATC CTTGAAAAAA ATAAAACGCA GCGTCAGCCC TCAAACTTTA 
GAAGCGTACA TACGTTGGAA CAAGGACTTT GGAGATACCA CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( NM_001363875.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001350804.1 Homo sapiens spastin (SPAST), transcript variant 4, mRNA.

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CloneID	OHu89825
Clone ID Related Accession (Same CDS sequence)	NM_001363875.1 , XM_017004777.1
Accession Version	NM_001363875.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	spastin isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121655.1 and AL121658.4. On May 30, 2018 this sequence version replaced XM_017004777.2. Transcript Variant: This variant (4) has multiple differences in the coding region, compared to variant 1. It encodes a shorter isoform (4), compared to isoform 1. This variant is also predicted to use an alternate, in-frame, downstream translation initiation site to encode an even shorter isoform. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.206341.1, SRR1803611.127602.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG 
AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT 
GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG 
ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAAAGTGG AGCTGTTCCA 
AAAAGAAAAG ACCCCTTAAC ACACACTAGT AATTCACTGC CTCGTTCAAA AACAGTTATG 
AAAACTGGAT CTGCAGGCCT TTCAGGCCAC CATAGAGCAC CTAGTTACAG TGGTTTATCC 
ATGGTTTCTG GAGTGAAACA GGGATCTGGT CCTGCTCCTA CCACTCATAA GGGTACTCCG 
AAAACAAATA GGACAAATAA ACCTTCTACC CCTACAACTG CTACTCGTAA GAAAAAAGAC 
TTGAAGAATT TTAGGAATGT GGACAGCAAC CTTGCTAACC TTATAATGAA TGAAATTGTG 
GACAATGGAA CAGCTGTTAA ATTTGATGAT ATAGCTGGTC AAGACTTGGC AAAACAAGCA 
TTGCAAGAAA TTGTTATTCT TCCTTCTCTG AGGCCTGAGT TGTTCACAGG GCTTAGAGCT 
CCTGCCAGAG GGCTGTTACT CTTTGGTCCA CCTGGGAATG GGAAGACAAT GCTGGCTAAA 
GCAGTAGCTG CAGAATCGAA TGCAACCTTC TTTAATATAA GTGCTGCAAG TTTAACTTCA 
AAATACGTGG GAGAAGGAGA GAAATTGGTG AGGGCTCTTT TTGCTGTGGC TCGAGAACTT 
CAACCTTCTA TAATTTTTAT AGATGAAGTT GATAGCCTTT TGTGTGAAAG AAGAGAAGGG 
GAGCACGATG CTAGTAGACG CCTAAAAACT GAATTTCTAA TAGAATTTGA TGGTGTACAG 
TCTGCTGGAG ATGACAGAGT ACTTGTAATG GGTGCAACTA ATAGGCCACA AGAGCTTGAT 
GAGGCTGTTC TCAGGCGTTT CATCAAACGG GTATATGTGT CTTTACCAAA TGAGGAGACA 
AGACTACTTT TGCTTAAAAA TCTGTTATGT AAACAAGGAA GTCCATTGAC CCAAAAAGAA 
CTAGCACAAC TTGCTAGAAT GACTGATGGA TACTCAGGAA GTGACCTAAC AGCTTTGGCA 
AAAGATGCAG CACTGGGTCC TATCCGAGAA CTAAAACCAG AACAGGTGAA GAATATGTCT 
GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACTG AATCCTTGAA AAAAATAAAA 
CGCAGCGTCA GCCCTCAAAC TTTAGAAGCG TACATACGTT GGAACAAGGA CTTTGGAGAT 
ACCACTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001350804.1
CDS	222..1973
Translation	MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	NM_001363875.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spastin (SPAST), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001363875.1

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG 
AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT 
GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG 
ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAAAGTGG AGCTGTTCCA 
AAAAGAAAAG ACCCCTTAAC ACACACTAGT AATTCACTGC CTCGTTCAAA AACAGTTATG 
AAAACTGGAT CTGCAGGCCT TTCAGGCCAC CATAGAGCAC CTAGTTACAG TGGTTTATCC 
ATGGTTTCTG GAGTGAAACA GGGATCTGGT CCTGCTCCTA CCACTCATAA GGGTACTCCG 
AAAACAAATA GGACAAATAA ACCTTCTACC CCTACAACTG CTACTCGTAA GAAAAAAGAC 
TTGAAGAATT TTAGGAATGT GGACAGCAAC CTTGCTAACC TTATAATGAA TGAAATTGTG 
GACAATGGAA CAGCTGTTAA ATTTGATGAT ATAGCTGGTC AAGACTTGGC AAAACAAGCA 
TTGCAAGAAA TTGTTATTCT TCCTTCTCTG AGGCCTGAGT TGTTCACAGG GCTTAGAGCT 
CCTGCCAGAG GGCTGTTACT CTTTGGTCCA CCTGGGAATG GGAAGACAAT GCTGGCTAAA 
GCAGTAGCTG CAGAATCGAA TGCAACCTTC TTTAATATAA GTGCTGCAAG TTTAACTTCA 
AAATACGTGG GAGAAGGAGA GAAATTGGTG AGGGCTCTTT TTGCTGTGGC TCGAGAACTT 
CAACCTTCTA TAATTTTTAT AGATGAAGTT GATAGCCTTT TGTGTGAAAG AAGAGAAGGG 
GAGCACGATG CTAGTAGACG CCTAAAAACT GAATTTCTAA TAGAATTTGA TGGTGTACAG 
TCTGCTGGAG ATGACAGAGT ACTTGTAATG GGTGCAACTA ATAGGCCACA AGAGCTTGAT 
GAGGCTGTTC TCAGGCGTTT CATCAAACGG GTATATGTGT CTTTACCAAA TGAGGAGACA 
AGACTACTTT TGCTTAAAAA TCTGTTATGT AAACAAGGAA GTCCATTGAC CCAAAAAGAA 
CTAGCACAAC TTGCTAGAAT GACTGATGGA TACTCAGGAA GTGACCTAAC AGCTTTGGCA 
AAAGATGCAG CACTGGGTCC TATCCGAGAA CTAAAACCAG AACAGGTGAA GAATATGTCT 
GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACTG AATCCTTGAA AAAAATAAAA 
CGCAGCGTCA GCCCTCAAAC TTTAGAAGCG TACATACGTT GGAACAAGGA CTTTGGAGAT 
ACCACTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( XM_005264516.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264573.1 Homo sapiens spastin (SPAST), transcript variant X1, mRNA.

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CloneID	OHu35573
Clone ID Related Accession (Same CDS sequence)	XM_005264516.4 , NM_001363823.1 , XM_005264516.5
Accession Version	XM_005264516.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spastin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264516.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG 
AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT 
GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG 
ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAGAAGAT GCAACCAGTT 
TTGCCATTTT CCAAGTCACA AACGGACGTC TATAATGACA GTACTAACTT GGCATGCCGC 
AATGGACATC TCCAGTCAGA AAGTGGAGCT GTTCCAAAAA GAAAAGACCC CTTAACACAC 
ACTAGTAATT CACTGCCTCG TTCAAAAACA GTTATGAAAA CTGGATCTGC AGGCCTTTCA 
GGCCACCATA GAGCACCTAG TTACAGTGGT TTATCCATGG TTTCTGGAGT GAAACAGGGA 
TCTGGTCCTG CTCCTACCAC TCATAAGGGT ACTCCGAAAA CAAATAGGAC AAATAAACCT 
TCTACCCCTA CAACTGCTAC TCGTAAGAAA AAAGACTTGA AGAATTTTAG GAATGTGGAC 
AGCAACCTTG CTAACCTTAT AATGAATGAA ATTGTGGACA ATGGAACAGC TGTTAAATTT 
GATGATATAG CTGGTCAAGA CTTGGCAAAA CAAGCATTGC AAGAAATTGT TATTCTTCCT 
TCTCTGAGGC CTGAGTTGTT CACAGGGCTT AGAGCTCCTG CCAGAGGGCT GTTACTCTTT 
GGTCCACCTG GGAATGGGAA GACAATGCTG GCTAAAGCAG TAGCTGCAGA ATCGAATGCA 
ACCTTCTTTA ATATAAGTGC TGCAAGTTTA ACTTCAAAAT ACGTGGGAGA AGGAGAGAAA 
TTGGTGAGGG CTCTTTTTGC TGTGGCTCGA GAACTTCAAC CTTCTATAAT TTTTATAGAT 
GAAGTTGATA GCCTTTTGTG TGAAAGAAGA GAAGGGGAGC ACGATGCTAG TAGACGCCTA 
AAAACTGAAT TTCTAATAGA ATTTGATGGT GTACAGTCTG CTGGAGATGA CAGAGTACTT 
GTAATGGGTG CAACTAATAG GCCACAAGAG CTTGATGAGG CTGTTCTCAG GCGTTTCATC 
AAACGGGTAT ATGTGTCTTT ACCAAATGAG GAGACAAGAC TACTTTTGCT TAAAAATCTG 
TTATGTAAAC AAGGAAGTCC ATTGACCCAA AAAGAACTAG CACAACTTGC TAGAATGACT 
GATGGATACT CAGGAAGTGA CCTAACAGCT TTGGCAAAAG ATGCAGCACT GGGTCCTATC 
CGAGAACTAA AACCAGAACA GGTGAAGAAT ATGTCTGCCA GTGAGATGAG AAATATTCGA 
TTATCTGACT TCACTGAATC CTTGAAAAAA ATAAAACGCA GCGTCAGCCC TCAAACTTTA 
GAAGCGTACA TACGTTGGAA CAAGGACTTT GGAGATACCA CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264573.1
CDS	1..1848
Translation	MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLEKMQPVLPFSKSQTDVYNDSTNLACRNGHLQSESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_005264516.4
Organism	Homo sapiens(human)
Definition	Homo sapiens spastin (SPAST), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264516.4

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG 
AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT 
GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG 
ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAGAAGAT GCAACCAGTT 
TTGCCATTTT CCAAGTCACA AACGGACGTC TATAATGACA GTACTAACTT GGCATGCCGC 
AATGGACATC TCCAGTCAGA AAGTGGAGCT GTTCCAAAAA GAAAAGACCC CTTAACACAC 
ACTAGTAATT CACTGCCTCG TTCAAAAACA GTTATGAAAA CTGGATCTGC AGGCCTTTCA 
GGCCACCATA GAGCACCTAG TTACAGTGGT TTATCCATGG TTTCTGGAGT GAAACAGGGA 
TCTGGTCCTG CTCCTACCAC TCATAAGGGT ACTCCGAAAA CAAATAGGAC AAATAAACCT 
TCTACCCCTA CAACTGCTAC TCGTAAGAAA AAAGACTTGA AGAATTTTAG GAATGTGGAC 
AGCAACCTTG CTAACCTTAT AATGAATGAA ATTGTGGACA ATGGAACAGC TGTTAAATTT 
GATGATATAG CTGGTCAAGA CTTGGCAAAA CAAGCATTGC AAGAAATTGT TATTCTTCCT 
TCTCTGAGGC CTGAGTTGTT CACAGGGCTT AGAGCTCCTG CCAGAGGGCT GTTACTCTTT 
GGTCCACCTG GGAATGGGAA GACAATGCTG GCTAAAGCAG TAGCTGCAGA ATCGAATGCA 
ACCTTCTTTA ATATAAGTGC TGCAAGTTTA ACTTCAAAAT ACGTGGGAGA AGGAGAGAAA 
TTGGTGAGGG CTCTTTTTGC TGTGGCTCGA GAACTTCAAC CTTCTATAAT TTTTATAGAT 
GAAGTTGATA GCCTTTTGTG TGAAAGAAGA GAAGGGGAGC ACGATGCTAG TAGACGCCTA 
AAAACTGAAT TTCTAATAGA ATTTGATGGT GTACAGTCTG CTGGAGATGA CAGAGTACTT 
GTAATGGGTG CAACTAATAG GCCACAAGAG CTTGATGAGG CTGTTCTCAG GCGTTTCATC 
AAACGGGTAT ATGTGTCTTT ACCAAATGAG GAGACAAGAC TACTTTTGCT TAAAAATCTG 
TTATGTAAAC AAGGAAGTCC ATTGACCCAA AAAGAACTAG CACAACTTGC TAGAATGACT 
GATGGATACT CAGGAAGTGA CCTAACAGCT TTGGCAAAAG ATGCAGCACT GGGTCCTATC 
CGAGAACTAA AACCAGAACA GGTGAAGAAT ATGTCTGCCA GTGAGATGAG AAATATTCGA 
TTATCTGACT TCACTGAATC CTTGAAAAAA ATAAAACGCA GCGTCAGCCC TCAAACTTTA 
GAAGCGTACA TACGTTGGAA CAAGGACTTT GGAGATACCA CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( XM_005264516.5 ) cDNA ORF clone, Homo sapiens(human) -> XP_005264573.1 Homo sapiens spastin (SPAST), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu35573
Clone ID Related Accession (Same CDS sequence)	XM_005264516.4 , NM_001363823.1 , XM_005264516.5
Accession Version	XM_005264516.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1848bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	spastin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005264516.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG 
AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT 
GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG 
ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAGAAGAT GCAACCAGTT 
TTGCCATTTT CCAAGTCACA AACGGACGTC TATAATGACA GTACTAACTT GGCATGCCGC 
AATGGACATC TCCAGTCAGA AAGTGGAGCT GTTCCAAAAA GAAAAGACCC CTTAACACAC 
ACTAGTAATT CACTGCCTCG TTCAAAAACA GTTATGAAAA CTGGATCTGC AGGCCTTTCA 
GGCCACCATA GAGCACCTAG TTACAGTGGT TTATCCATGG TTTCTGGAGT GAAACAGGGA 
TCTGGTCCTG CTCCTACCAC TCATAAGGGT ACTCCGAAAA CAAATAGGAC AAATAAACCT 
TCTACCCCTA CAACTGCTAC TCGTAAGAAA AAAGACTTGA AGAATTTTAG GAATGTGGAC 
AGCAACCTTG CTAACCTTAT AATGAATGAA ATTGTGGACA ATGGAACAGC TGTTAAATTT 
GATGATATAG CTGGTCAAGA CTTGGCAAAA CAAGCATTGC AAGAAATTGT TATTCTTCCT 
TCTCTGAGGC CTGAGTTGTT CACAGGGCTT AGAGCTCCTG CCAGAGGGCT GTTACTCTTT 
GGTCCACCTG GGAATGGGAA GACAATGCTG GCTAAAGCAG TAGCTGCAGA ATCGAATGCA 
ACCTTCTTTA ATATAAGTGC TGCAAGTTTA ACTTCAAAAT ACGTGGGAGA AGGAGAGAAA 
TTGGTGAGGG CTCTTTTTGC TGTGGCTCGA GAACTTCAAC CTTCTATAAT TTTTATAGAT 
GAAGTTGATA GCCTTTTGTG TGAAAGAAGA GAAGGGGAGC ACGATGCTAG TAGACGCCTA 
AAAACTGAAT TTCTAATAGA ATTTGATGGT GTACAGTCTG CTGGAGATGA CAGAGTACTT 
GTAATGGGTG CAACTAATAG GCCACAAGAG CTTGATGAGG CTGTTCTCAG GCGTTTCATC 
AAACGGGTAT ATGTGTCTTT ACCAAATGAG GAGACAAGAC TACTTTTGCT TAAAAATCTG 
TTATGTAAAC AAGGAAGTCC ATTGACCCAA AAAGAACTAG CACAACTTGC TAGAATGACT 
GATGGATACT CAGGAAGTGA CCTAACAGCT TTGGCAAAAG ATGCAGCACT GGGTCCTATC 
CGAGAACTAA AACCAGAACA GGTGAAGAAT ATGTCTGCCA GTGAGATGAG AAATATTCGA 
TTATCTGACT TCACTGAATC CTTGAAAAAA ATAAAACGCA GCGTCAGCCC TCAAACTTTA 
GAAGCGTACA TACGTTGGAA CAAGGACTTT GGAGATACCA CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005264573.1
CDS	1..1848
Translation	MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLEKMQPVLPFSKSQTDVYNDSTNLACRNGHLQSESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_005264516.5
Organism	Homo sapiens(human)
Definition	Homo sapiens spastin (SPAST), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005264516.5

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG 
AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT 
GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG 
ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAGAAGAT GCAACCAGTT 
TTGCCATTTT CCAAGTCACA AACGGACGTC TATAATGACA GTACTAACTT GGCATGCCGC 
AATGGACATC TCCAGTCAGA AAGTGGAGCT GTTCCAAAAA GAAAAGACCC CTTAACACAC 
ACTAGTAATT CACTGCCTCG TTCAAAAACA GTTATGAAAA CTGGATCTGC AGGCCTTTCA 
GGCCACCATA GAGCACCTAG TTACAGTGGT TTATCCATGG TTTCTGGAGT GAAACAGGGA 
TCTGGTCCTG CTCCTACCAC TCATAAGGGT ACTCCGAAAA CAAATAGGAC AAATAAACCT 
TCTACCCCTA CAACTGCTAC TCGTAAGAAA AAAGACTTGA AGAATTTTAG GAATGTGGAC 
AGCAACCTTG CTAACCTTAT AATGAATGAA ATTGTGGACA ATGGAACAGC TGTTAAATTT 
GATGATATAG CTGGTCAAGA CTTGGCAAAA CAAGCATTGC AAGAAATTGT TATTCTTCCT 
TCTCTGAGGC CTGAGTTGTT CACAGGGCTT AGAGCTCCTG CCAGAGGGCT GTTACTCTTT 
GGTCCACCTG GGAATGGGAA GACAATGCTG GCTAAAGCAG TAGCTGCAGA ATCGAATGCA 
ACCTTCTTTA ATATAAGTGC TGCAAGTTTA ACTTCAAAAT ACGTGGGAGA AGGAGAGAAA 
TTGGTGAGGG CTCTTTTTGC TGTGGCTCGA GAACTTCAAC CTTCTATAAT TTTTATAGAT 
GAAGTTGATA GCCTTTTGTG TGAAAGAAGA GAAGGGGAGC ACGATGCTAG TAGACGCCTA 
AAAACTGAAT TTCTAATAGA ATTTGATGGT GTACAGTCTG CTGGAGATGA CAGAGTACTT 
GTAATGGGTG CAACTAATAG GCCACAAGAG CTTGATGAGG CTGTTCTCAG GCGTTTCATC 
AAACGGGTAT ATGTGTCTTT ACCAAATGAG GAGACAAGAC TACTTTTGCT TAAAAATCTG 
TTATGTAAAC AAGGAAGTCC ATTGACCCAA AAAGAACTAG CACAACTTGC TAGAATGACT 
GATGGATACT CAGGAAGTGA CCTAACAGCT TTGGCAAAAG ATGCAGCACT GGGTCCTATC 
CGAGAACTAA AACCAGAACA GGTGAAGAAT ATGTCTGCCA GTGAGATGAG AAATATTCGA 
TTATCTGACT TCACTGAATC CTTGAAAAAA ATAAAACGCA GCGTCAGCCC TCAAACTTTA 
GAAGCGTACA TACGTTGGAA CAAGGACTTT GGAGATACCA CTGTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( XM_011533067.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531369.1 Homo sapiens spastin (SPAST), transcript variant X3, mRNA.

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CloneID	OHu60708
Clone ID Related Accession (Same CDS sequence)	XM_011533067.2
Accession Version	XM_011533067.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1656bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spastin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533067.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA 
CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA 
GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA 
ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAGAA GATGCAACCA 
GTTTTGCCAT TTTCCAAGTC ACAAACGGAC GTCTATAATG ACAGTACTAA CTTGGCATGC 
CGCAATGGAC ATCTCCAGTC AGAAAGTGGA GCTGTTCCAA AAAGAAAAGA CCCCTTAACA 
CACACTAGTA ATTCACTGCC TCGTTCAAAA ACAGTTATGA AAACTGGATC TGCAGGCCTT 
TCAGGCCACC ATAGAGCACC TAGTTACAGT GGTTTATCCA TGGTTTCTGG AGTGAAACAG 
GGATCTGGTC CTGCTCCTAC CACTCATAAG GGTACTCCGA AAACAAATAG GACAAATAAA 
CCTTCTACCC CTACAACTGC TACTCGTAAG AAAAAAGACT TGAAGAATTT TAGGAATGTG 
GACAGCAACC TTGCTAACCT TATAATGAAT GAAATTGTGG ACAATGGAAC AGCTGTTAAA 
TTTGATGATA TAGCTGGTCA AGACTTGGCA AAACAAGCAT TGCAAGAAAT TGTTATTCTT 
CCTTCTCTGA GGCCTGAGTT GTTCACAGGG CTTAGAGCTC CTGCCAGAGG GCTGTTACTC 
TTTGGTCCAC CTGGGAATGG GAAGACAATG CTGGCTAAAG CAGTAGCTGC AGAATCGAAT 
GCAACCTTCT TTAATATAAG TGCTGCAAGT TTAACTTCAA AATACGTGGG AGAAGGAGAG 
AAATTGGTGA GGGCTCTTTT TGCTGTGGCT CGAGAACTTC AACCTTCTAT AATTTTTATA 
GATGAAGTTG ATAGCCTTTT GTGTGAAAGA AGAGAAGGGG AGCACGATGC TAGTAGACGC 
CTAAAAACTG AATTTCTAAT AGAATTTGAT GGTGTACAGT CTGCTGGAGA TGACAGAGTA 
CTTGTAATGG GTGCAACTAA TAGGCCACAA GAGCTTGATG AGGCTGTTCT CAGGCGTTTC 
ATCAAACGGG TATATGTGTC TTTACCAAAT GAGGAGACAA GACTACTTTT GCTTAAAAAT 
CTGTTATGTA AACAAGGAAG TCCATTGACC CAAAAAGAAC TAGCACAACT TGCTAGAACT 
AAAACCAGAA CAGGTGAAGA ATATGTCTGC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531369.1
CDS	282..1937
Translation	MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLEKMQPVLPFSKSQTDVYNDSTNLACRNGHLQSESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARTKTRTGEEYVCQ

Target ORF information:

RefSeq Version	XM_011533067.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spastin (SPAST), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533067.2

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA 
CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA 
GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA 
ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAGAA GATGCAACCA 
GTTTTGCCAT TTTCCAAGTC ACAAACGGAC GTCTATAATG ACAGTACTAA CTTGGCATGC 
CGCAATGGAC ATCTCCAGTC AGAAAGTGGA GCTGTTCCAA AAAGAAAAGA CCCCTTAACA 
CACACTAGTA ATTCACTGCC TCGTTCAAAA ACAGTTATGA AAACTGGATC TGCAGGCCTT 
TCAGGCCACC ATAGAGCACC TAGTTACAGT GGTTTATCCA TGGTTTCTGG AGTGAAACAG 
GGATCTGGTC CTGCTCCTAC CACTCATAAG GGTACTCCGA AAACAAATAG GACAAATAAA 
CCTTCTACCC CTACAACTGC TACTCGTAAG AAAAAAGACT TGAAGAATTT TAGGAATGTG 
GACAGCAACC TTGCTAACCT TATAATGAAT GAAATTGTGG ACAATGGAAC AGCTGTTAAA 
TTTGATGATA TAGCTGGTCA AGACTTGGCA AAACAAGCAT TGCAAGAAAT TGTTATTCTT 
CCTTCTCTGA GGCCTGAGTT GTTCACAGGG CTTAGAGCTC CTGCCAGAGG GCTGTTACTC 
TTTGGTCCAC CTGGGAATGG GAAGACAATG CTGGCTAAAG CAGTAGCTGC AGAATCGAAT 
GCAACCTTCT TTAATATAAG TGCTGCAAGT TTAACTTCAA AATACGTGGG AGAAGGAGAG 
AAATTGGTGA GGGCTCTTTT TGCTGTGGCT CGAGAACTTC AACCTTCTAT AATTTTTATA 
GATGAAGTTG ATAGCCTTTT GTGTGAAAGA AGAGAAGGGG AGCACGATGC TAGTAGACGC 
CTAAAAACTG AATTTCTAAT AGAATTTGAT GGTGTACAGT CTGCTGGAGA TGACAGAGTA 
CTTGTAATGG GTGCAACTAA TAGGCCACAA GAGCTTGATG AGGCTGTTCT CAGGCGTTTC 
ATCAAACGGG TATATGTGTC TTTACCAAAT GAGGAGACAA GACTACTTTT GCTTAAAAAT 
CTGTTATGTA AACAAGGAAG TCCATTGACC CAAAAAGAAC TAGCACAACT TGCTAGAACT 
AAAACCAGAA CAGGTGAAGA ATATGTCTGC CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( XM_017004777.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860266.1 Homo sapiens spastin (SPAST), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu89825
Clone ID Related Accession (Same CDS sequence)	NM_001363875.1 , XM_017004777.1
Accession Version	XM_017004777.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1752bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spastin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG 
AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT 
GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG 
ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAAAGTGG AGCTGTTCCA 
AAAAGAAAAG ACCCCTTAAC ACACACTAGT AATTCACTGC CTCGTTCAAA AACAGTTATG 
AAAACTGGAT CTGCAGGCCT TTCAGGCCAC CATAGAGCAC CTAGTTACAG TGGTTTATCC 
ATGGTTTCTG GAGTGAAACA GGGATCTGGT CCTGCTCCTA CCACTCATAA GGGTACTCCG 
AAAACAAATA GGACAAATAA ACCTTCTACC CCTACAACTG CTACTCGTAA GAAAAAAGAC 
TTGAAGAATT TTAGGAATGT GGACAGCAAC CTTGCTAACC TTATAATGAA TGAAATTGTG 
GACAATGGAA CAGCTGTTAA ATTTGATGAT ATAGCTGGTC AAGACTTGGC AAAACAAGCA 
TTGCAAGAAA TTGTTATTCT TCCTTCTCTG AGGCCTGAGT TGTTCACAGG GCTTAGAGCT 
CCTGCCAGAG GGCTGTTACT CTTTGGTCCA CCTGGGAATG GGAAGACAAT GCTGGCTAAA 
GCAGTAGCTG CAGAATCGAA TGCAACCTTC TTTAATATAA GTGCTGCAAG TTTAACTTCA 
AAATACGTGG GAGAAGGAGA GAAATTGGTG AGGGCTCTTT TTGCTGTGGC TCGAGAACTT 
CAACCTTCTA TAATTTTTAT AGATGAAGTT GATAGCCTTT TGTGTGAAAG AAGAGAAGGG 
GAGCACGATG CTAGTAGACG CCTAAAAACT GAATTTCTAA TAGAATTTGA TGGTGTACAG 
TCTGCTGGAG ATGACAGAGT ACTTGTAATG GGTGCAACTA ATAGGCCACA AGAGCTTGAT 
GAGGCTGTTC TCAGGCGTTT CATCAAACGG GTATATGTGT CTTTACCAAA TGAGGAGACA 
AGACTACTTT TGCTTAAAAA TCTGTTATGT AAACAAGGAA GTCCATTGAC CCAAAAAGAA 
CTAGCACAAC TTGCTAGAAT GACTGATGGA TACTCAGGAA GTGACCTAAC AGCTTTGGCA 
AAAGATGCAG CACTGGGTCC TATCCGAGAA CTAAAACCAG AACAGGTGAA GAATATGTCT 
GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACTG AATCCTTGAA AAAAATAAAA 
CGCAGCGTCA GCCCTCAAAC TTTAGAAGCG TACATACGTT GGAACAAGGA CTTTGGAGAT 
ACCACTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860266.1
CDS	282..2033
Translation	MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARMTDGYSGSDLTALAKDAALGPIRELKPEQVKNMSASEMRNIRLSDFTESLKKIKRSVSPQTLEAYIRWNKDFGDTTV

Target ORF information:

RefSeq Version	XM_017004777.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spastin (SPAST), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004777.1

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG 
AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT 
GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG 
ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAAAGTGG AGCTGTTCCA 
AAAAGAAAAG ACCCCTTAAC ACACACTAGT AATTCACTGC CTCGTTCAAA AACAGTTATG 
AAAACTGGAT CTGCAGGCCT TTCAGGCCAC CATAGAGCAC CTAGTTACAG TGGTTTATCC 
ATGGTTTCTG GAGTGAAACA GGGATCTGGT CCTGCTCCTA CCACTCATAA GGGTACTCCG 
AAAACAAATA GGACAAATAA ACCTTCTACC CCTACAACTG CTACTCGTAA GAAAAAAGAC 
TTGAAGAATT TTAGGAATGT GGACAGCAAC CTTGCTAACC TTATAATGAA TGAAATTGTG 
GACAATGGAA CAGCTGTTAA ATTTGATGAT ATAGCTGGTC AAGACTTGGC AAAACAAGCA 
TTGCAAGAAA TTGTTATTCT TCCTTCTCTG AGGCCTGAGT TGTTCACAGG GCTTAGAGCT 
CCTGCCAGAG GGCTGTTACT CTTTGGTCCA CCTGGGAATG GGAAGACAAT GCTGGCTAAA 
GCAGTAGCTG CAGAATCGAA TGCAACCTTC TTTAATATAA GTGCTGCAAG TTTAACTTCA 
AAATACGTGG GAGAAGGAGA GAAATTGGTG AGGGCTCTTT TTGCTGTGGC TCGAGAACTT 
CAACCTTCTA TAATTTTTAT AGATGAAGTT GATAGCCTTT TGTGTGAAAG AAGAGAAGGG 
GAGCACGATG CTAGTAGACG CCTAAAAACT GAATTTCTAA TAGAATTTGA TGGTGTACAG 
TCTGCTGGAG ATGACAGAGT ACTTGTAATG GGTGCAACTA ATAGGCCACA AGAGCTTGAT 
GAGGCTGTTC TCAGGCGTTT CATCAAACGG GTATATGTGT CTTTACCAAA TGAGGAGACA 
AGACTACTTT TGCTTAAAAA TCTGTTATGT AAACAAGGAA GTCCATTGAC CCAAAAAGAA 
CTAGCACAAC TTGCTAGAAT GACTGATGGA TACTCAGGAA GTGACCTAAC AGCTTTGGCA 
AAAGATGCAG CACTGGGTCC TATCCGAGAA CTAAAACCAG AACAGGTGAA GAATATGTCT 
GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACTG AATCCTTGAA AAAAATAAAA 
CGCAGCGTCA GCCCTCAAAC TTTAGAAGCG TACATACGTT GGAACAAGGA CTTTGGAGAT 
ACCACTGTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( XM_017004778.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860267.1 Homo sapiens spastin (SPAST), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OHu89826
Clone ID Related Accession (Same CDS sequence)	XM_017004778.1 , XM_017004778.2
Accession Version	XM_017004778.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	spastin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA 
CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA 
GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA 
ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAAAG TGGAGCTGTT 
CCAAAAAGAA AAGACCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT 
ATGAAAACTG GATCTGCAGG CCTTTCAGGC CACCATAGAG CACCTAGTTA CAGTGGTTTA 
TCCATGGTTT CTGGAGTGAA ACAGGGATCT GGTCCTGCTC CTACCACTCA TAAGGGTACT 
CCGAAAACAA ATAGGACAAA TAAACCTTCT ACCCCTACAA CTGCTACTCG TAAGAAAAAA 
GACTTGAAGA ATTTTAGGAA TGTGGACAGC AACCTTGCTA ACCTTATAAT GAATGAAATT 
GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GTCAAGACTT GGCAAAACAA 
GCATTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA 
GCTCCTGCCA GAGGGCTGTT ACTCTTTGGT CCACCTGGGA ATGGGAAGAC AATGCTGGCT 
AAAGCAGTAG CTGCAGAATC GAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT 
TCAAAATACG TGGGAGAAGG AGAGAAATTG GTGAGGGCTC TTTTTGCTGT GGCTCGAGAA 
CTTCAACCTT CTATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA 
GGGGAGCACG ATGCTAGTAG ACGCCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA 
CAGTCTGCTG GAGATGACAG AGTACTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT 
GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTACC AAATGAGGAG 
ACAAGACTAC TTTTGCTTAA AAATCTGTTA TGTAAACAAG GAAGTCCATT GACCCAAAAA 
GAACTAGCAC AACTTGCTAG AACTAAAACC AGAACAGGTG AAGAATATGT CTGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860267.1
CDS	282..1841
Translation	MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARTKTRTGEEYVCQ

Target ORF information:

RefSeq Version	XM_017004778.1
Organism	Homo sapiens(human)
Definition	Homo sapiens spastin (SPAST), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004778.1

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA 
CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA 
GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA 
ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAAAG TGGAGCTGTT 
CCAAAAAGAA AAGACCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT 
ATGAAAACTG GATCTGCAGG CCTTTCAGGC CACCATAGAG CACCTAGTTA CAGTGGTTTA 
TCCATGGTTT CTGGAGTGAA ACAGGGATCT GGTCCTGCTC CTACCACTCA TAAGGGTACT 
CCGAAAACAA ATAGGACAAA TAAACCTTCT ACCCCTACAA CTGCTACTCG TAAGAAAAAA 
GACTTGAAGA ATTTTAGGAA TGTGGACAGC AACCTTGCTA ACCTTATAAT GAATGAAATT 
GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GTCAAGACTT GGCAAAACAA 
GCATTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA 
GCTCCTGCCA GAGGGCTGTT ACTCTTTGGT CCACCTGGGA ATGGGAAGAC AATGCTGGCT 
AAAGCAGTAG CTGCAGAATC GAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT 
TCAAAATACG TGGGAGAAGG AGAGAAATTG GTGAGGGCTC TTTTTGCTGT GGCTCGAGAA 
CTTCAACCTT CTATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA 
GGGGAGCACG ATGCTAGTAG ACGCCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA 
CAGTCTGCTG GAGATGACAG AGTACTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT 
GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTACC AAATGAGGAG 
ACAAGACTAC TTTTGCTTAA AAATCTGTTA TGTAAACAAG GAAGTCCATT GACCCAAAAA 
GAACTAGCAC AACTTGCTAG AACTAAAACC AGAACAGGTG AAGAATATGT CTGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPAST ( XM_017004778.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016860267.1 Homo sapiens spastin (SPAST), transcript variant X4, mRNA.

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CloneID	OHu89826
Clone ID Related Accession (Same CDS sequence)	XM_017004778.1 , XM_017004778.2
Accession Version	XM_017004778.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1560bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	spastin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017004778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA 
CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA 
GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA 
ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAAAG TGGAGCTGTT 
CCAAAAAGAA AAGACCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT 
ATGAAAACTG GATCTGCAGG CCTTTCAGGC CACCATAGAG CACCTAGTTA CAGTGGTTTA 
TCCATGGTTT CTGGAGTGAA ACAGGGATCT GGTCCTGCTC CTACCACTCA TAAGGGTACT 
CCGAAAACAA ATAGGACAAA TAAACCTTCT ACCCCTACAA CTGCTACTCG TAAGAAAAAA 
GACTTGAAGA ATTTTAGGAA TGTGGACAGC AACCTTGCTA ACCTTATAAT GAATGAAATT 
GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GTCAAGACTT GGCAAAACAA 
GCATTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA 
GCTCCTGCCA GAGGGCTGTT ACTCTTTGGT CCACCTGGGA ATGGGAAGAC AATGCTGGCT 
AAAGCAGTAG CTGCAGAATC GAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT 
TCAAAATACG TGGGAGAAGG AGAGAAATTG GTGAGGGCTC TTTTTGCTGT GGCTCGAGAA 
CTTCAACCTT CTATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA 
GGGGAGCACG ATGCTAGTAG ACGCCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA 
CAGTCTGCTG GAGATGACAG AGTACTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT 
GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTACC AAATGAGGAG 
ACAAGACTAC TTTTGCTTAA AAATCTGTTA TGTAAACAAG GAAGTCCATT GACCCAAAAA 
GAACTAGCAC AACTTGCTAG AACTAAAACC AGAACAGGTG AAGAATATGT CTGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016860267.1
CDS	282..1841
Translation	MNSPGGRGKKKGSGGASNPVPPRPPPPCLAPAPPAAGPAPPPESPHKRNLYYFSYPLFVGFALLRLVAFHLGLLFVWLCQRFSRALMAAKRSSGAAPAPASASAPAPVPGGEAERVRVFHKQAFEYISIALRIDEDEKAGQKEQAVEWYKKGIEELEKGIAVIVTGQGEQCERARRLQAKMMTNLVMAKDRLQLLESGAVPKRKDPLTHTSNSLPRSKTVMKTGSAGLSGHHRAPSYSGLSMVSGVKQGSGPAPTTHKGTPKTNRTNKPSTPTTATRKKKDLKNFRNVDSNLANLIMNEIVDNGTAVKFDDIAGQDLAKQALQEIVILPSLRPELFTGLRAPARGLLLFGPPGNGKTMLAKAVAAESNATFFNISAASLTSKYVGEGEKLVRALFAVARELQPSIIFIDEVDSLLCERREGEHDASRRLKTEFLIEFDGVQSAGDDRVLVMGATNRPQELDEAVLRRFIKRVYVSLPNEETRLLLLKNLLCKQGSPLTQKELAQLARTKTRTGEEYVCQ

Target ORF information:

RefSeq Version	XM_017004778.2
Organism	Homo sapiens(human)
Definition	Homo sapiens spastin (SPAST), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017004778.2

ORF Insert Sequence:

ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT 
CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC 
TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG 
CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC 
TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC 
AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA 
CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA 
GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA 
ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAAAG TGGAGCTGTT 
CCAAAAAGAA AAGACCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT 
ATGAAAACTG GATCTGCAGG CCTTTCAGGC CACCATAGAG CACCTAGTTA CAGTGGTTTA 
TCCATGGTTT CTGGAGTGAA ACAGGGATCT GGTCCTGCTC CTACCACTCA TAAGGGTACT 
CCGAAAACAA ATAGGACAAA TAAACCTTCT ACCCCTACAA CTGCTACTCG TAAGAAAAAA 
GACTTGAAGA ATTTTAGGAA TGTGGACAGC AACCTTGCTA ACCTTATAAT GAATGAAATT 
GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GTCAAGACTT GGCAAAACAA 
GCATTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA 
GCTCCTGCCA GAGGGCTGTT ACTCTTTGGT CCACCTGGGA ATGGGAAGAC AATGCTGGCT 
AAAGCAGTAG CTGCAGAATC GAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT 
TCAAAATACG TGGGAGAAGG AGAGAAATTG GTGAGGGCTC TTTTTGCTGT GGCTCGAGAA 
CTTCAACCTT CTATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA 
GGGGAGCACG ATGCTAGTAG ACGCCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA 
CAGTCTGCTG GAGATGACAG AGTACTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT 
GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTACC AAATGAGGAG 
ACAAGACTAC TTTTGCTTAA AAATCTGTTA TGTAAACAAG GAAGTCCATT GACCCAAAAA 
GAACTAGCAC AACTTGCTAG AACTAAAACC AGAACAGGTG AAGAATATGT CTGCCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	SPAST
Entrez Gene ID	6683
Full Name	spastin
Synonyms	ADPSP,FSP2,SPG4
General protein information	Preferred Names spastin Names spastin spastic paraplegia 4 (autosomal dominant; spastin) spastic paraplegia 4 protein
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p22.3
Summary	This gene encodes a member of the AAA (ATPases associated with a variety of cellular activities) protein family. Members of this protein family share an ATPase domain and have roles in diverse cellular processes including membrane trafficking, intracellular motility, organelle biogenesis, protein folding, and proteolysis. The encoded ATPase may be involved in the assembly or function of nuclear protein complexes. Two transcript variants encoding distinct isoforms have been identified for this gene. Other alternative splice variants have been described but their full length sequences have not been determined. Mutations associated with this gene cause the most frequent form of autosomal dominant spastic paraplegia 4. [provided by RefSeq, Jul 2008].
Disorder MIM:	604277
Disorder Html:	Spastic paraplegia 4, autosomal dominant, 182601 (3)

Homo sapiens (human)	SPAST	NP_055761.2
Oryza sativa (rice)	Os06g0130000	NP_001056686.1
Xenopus tropicalis (tropical clawed frog)	spast	NP_001016453.1
	SPAST	XP_002799248.1
Canis lupus familiaris (dog)	SPAST	XP_850973.1
Drosophila melanogaster (fruit fly)	spas	NP_651206.3
Pan troglodytes (chimpanzee)	SPAST	XP_001164367.1
Mus musculus (house mouse)	Spast	NP_001156342.1
Rattus norvegicus (Norway rat)	Spast	NP_001102172.2
Danio rerio (zebrafish)	spast	NP_998080.1
Arabidopsis thaliana (thale cress)	AT2G45500	NP_182074.4
Bos taurus (cattle)	SPAST	NP_001075060.1
Gallus gallus (chicken)	SPAST	NP_001026232.1
Anopheles gambiae (African malaria mosquito)	SPAST_ANOGA	XP_312634.5
Caenorhabditis elegans (roundworm)	spas-1	NP_741586.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

SPAST cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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