SPAST cDNA ORF clone, Homo sapiens(human)

The following SPAST gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAST cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu25324 NM_014946.3
Latest version!
Homo sapiens spastin (SPAST), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu25537 NM_199436.1
Latest version!
Homo sapiens spastin (SPAST), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu35573 NM_001363823.1
Latest version!
Homo sapiens spastin (SPAST), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu89825 NM_001363875.1
Latest version!
Homo sapiens spastin (SPAST), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu35573 XM_005264516.5
Latest version!
Homo sapiens spastin (SPAST), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu60708 XM_011533067.2
Latest version!
Homo sapiens spastin (SPAST), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu89825 XM_017004777.1
Latest version!
Homo sapiens spastin (SPAST), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu89826 XM_017004778.2
Latest version!
Homo sapiens spastin (SPAST), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
View Old Accession Versions >>
OHu89826 XM_017004778.1 Homo sapiens spastin (SPAST), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
OHu35573 XM_005264516.4 Homo sapiens spastin (SPAST), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $307.30
$439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu25324
    Clone ID Related Accession (Same CDS sequence) NM_014946.3
    Accession Version NM_014946.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1851bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product spastin isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB029006.1, AJ246001.1, BG532310.1 and AA830062.1. This sequence is a reference standard in the RefSeqGene project. On Jan 13, 2004 this sequence version replaced NM_014946.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1; also known as M1). It also encodes a shorter isoform (M87) from an alternate, in-frame, downstream translation initiation site. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.105119.1, SRR1803614.230472.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA
    CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA
    GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA
    ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAGAA GATGCAACCA
    GTTTTGCCAT TTTCCAAGTC ACAAACGGAC GTCTATAATG ACAGTACTAA CTTGGCATGC
    CGCAATGGAC ATCTCCAGTC AGAAAGTGGA GCTGTTCCAA AAAGAAAAGA CCCCTTAACA
    CACACTAGTA ATTCACTGCC TCGTTCAAAA ACAGTTATGA AAACTGGATC TGCAGGCCTT
    TCAGGCCACC ATAGAGCACC TAGTTACAGT GGTTTATCCA TGGTTTCTGG AGTGAAACAG
    GGATCTGGTC CTGCTCCTAC CACTCATAAG GGTACTCCGA AAACAAATAG GACAAATAAA
    CCTTCTACCC CTACAACTGC TACTCGTAAG AAAAAAGACT TGAAGAATTT TAGGAATGTG
    GACAGCAACC TTGCTAACCT TATAATGAAT GAAATTGTGG ACAATGGAAC AGCTGTTAAA
    TTTGATGATA TAGCTGGTCA AGACTTGGCA AAACAAGCAT TGCAAGAAAT TGTTATTCTT
    CCTTCTCTGA GGCCTGAGTT GTTCACAGGG CTTAGAGCTC CTGCCAGAGG GCTGTTACTC
    TTTGGTCCAC CTGGGAATGG GAAGACAATG CTGGCTAAAG CAGTAGCTGC AGAATCGAAT
    GCAACCTTCT TTAATATAAG TGCTGCAAGT TTAACTTCAA AATACGTGGG AGAAGGAGAG
    AAATTGGTGA GGGCTCTTTT TGCTGTGGCT CGAGAACTTC AACCTTCTAT AATTTTTATA
    GATGAAGTTG ATAGCCTTTT GTGTGAAAGA AGAGAAGGGG AGCACGATGC TAGTAGACGC
    CTAAAAACTG AATTTCTAAT AGAATTTGAT GGTGTACAGT CTGCTGGAGA TGACAGAGTA
    CTTGTAATGG GTGCAACTAA TAGGCCACAA GAGCTTGATG AGGCTGTTCT CAGGCGTTTC
    ATCAAACGGG TATATGTGTC TTTACCAAAT GAGGAGACAA GACTACTTTT GCTTAAAAAT
    CTGTTATGTA AACAAGGAAG TCCATTGACC CAAAAAGAAC TAGCACAACT TGCTAGAATG
    ACTGATGGAT ACTCAGGAAG TGACCTAACA GCTTTGGCAA AAGATGCAGC ACTGGGTCCT
    ATCCGAGAAC TAAAACCAGA ACAGGTGAAG AATATGTCTG CCAGTGAGAT GAGAAATATT
    CGATTATCTG ACTTCACTGA ATCCTTGAAA AAAATAAAAC GCAGCGTCAG CCCTCAAACT
    TTAGAAGCGT ACATACGTTG GAACAAGGAC TTTGGAGATA CCACTGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_055761.2
    CDS222..2072
    Translation

    Target ORF information:

    RefSeq Version NM_014946.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spastin (SPAST), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_014946.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA
    CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA
    GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA
    ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAGAA GATGCAACCA
    GTTTTGCCAT TTTCCAAGTC ACAAACGGAC GTCTATAATG ACAGTACTAA CTTGGCATGC
    CGCAATGGAC ATCTCCAGTC AGAAAGTGGA GCTGTTCCAA AAAGAAAAGA CCCCTTAACA
    CACACTAGTA ATTCACTGCC TCGTTCAAAA ACAGTTATGA AAACTGGATC TGCAGGCCTT
    TCAGGCCACC ATAGAGCACC TAGTTACAGT GGTTTATCCA TGGTTTCTGG AGTGAAACAG
    GGATCTGGTC CTGCTCCTAC CACTCATAAG GGTACTCCGA AAACAAATAG GACAAATAAA
    CCTTCTACCC CTACAACTGC TACTCGTAAG AAAAAAGACT TGAAGAATTT TAGGAATGTG
    GACAGCAACC TTGCTAACCT TATAATGAAT GAAATTGTGG ACAATGGAAC AGCTGTTAAA
    TTTGATGATA TAGCTGGTCA AGACTTGGCA AAACAAGCAT TGCAAGAAAT TGTTATTCTT
    CCTTCTCTGA GGCCTGAGTT GTTCACAGGG CTTAGAGCTC CTGCCAGAGG GCTGTTACTC
    TTTGGTCCAC CTGGGAATGG GAAGACAATG CTGGCTAAAG CAGTAGCTGC AGAATCGAAT
    GCAACCTTCT TTAATATAAG TGCTGCAAGT TTAACTTCAA AATACGTGGG AGAAGGAGAG
    AAATTGGTGA GGGCTCTTTT TGCTGTGGCT CGAGAACTTC AACCTTCTAT AATTTTTATA
    GATGAAGTTG ATAGCCTTTT GTGTGAAAGA AGAGAAGGGG AGCACGATGC TAGTAGACGC
    CTAAAAACTG AATTTCTAAT AGAATTTGAT GGTGTACAGT CTGCTGGAGA TGACAGAGTA
    CTTGTAATGG GTGCAACTAA TAGGCCACAA GAGCTTGATG AGGCTGTTCT CAGGCGTTTC
    ATCAAACGGG TATATGTGTC TTTACCAAAT GAGGAGACAA GACTACTTTT GCTTAAAAAT
    CTGTTATGTA AACAAGGAAG TCCATTGACC CAAAAAGAAC TAGCACAACT TGCTAGAATG
    ACTGATGGAT ACTCAGGAAG TGACCTAACA GCTTTGGCAA AAGATGCAGC ACTGGGTCCT
    ATCCGAGAAC TAAAACCAGA ACAGGTGAAG AATATGTCTG CCAGTGAGAT GAGAAATATT
    CGATTATCTG ACTTCACTGA ATCCTTGAAA AAAATAAAAC GCAGCGTCAG CCCTCAAACT
    TTAGAAGCGT ACATACGTTG GAACAAGGAC TTTGGAGATA CCACTGTTTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu25537
    Clone ID Related Accession (Same CDS sequence) NM_199436.1
    Accession Version NM_199436.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1755bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product spastin isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB029006.1, BG532310.1 and AA830062.1. Transcript Variant: This variant (2) lacks an in-frame segment of the coding region, compared to variant 1. It encodes a shorter isoform (2), compared to isoform 1. This variant is also predicted to use an alternate, in-frame, downstream translation initiation site to encode an even shorter isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC150260.1, SRR1660805.241629.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA
    CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA
    GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA
    ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAAAG TGGAGCTGTT
    CCAAAAAGAA AAGACCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT
    ATGAAAACTG GATCTGCAGG CCTTTCAGGC CACCATAGAG CACCTAGTTA CAGTGGTTTA
    TCCATGGTTT CTGGAGTGAA ACAGGGATCT GGTCCTGCTC CTACCACTCA TAAGGGTACT
    CCGAAAACAA ATAGGACAAA TAAACCTTCT ACCCCTACAA CTGCTACTCG TAAGAAAAAA
    GACTTGAAGA ATTTTAGGAA TGTGGACAGC AACCTTGCTA ACCTTATAAT GAATGAAATT
    GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GTCAAGACTT GGCAAAACAA
    GCATTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA
    GCTCCTGCCA GAGGGCTGTT ACTCTTTGGT CCACCTGGGA ATGGGAAGAC AATGCTGGCT
    AAAGCAGTAG CTGCAGAATC GAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT
    TCAAAATACG TGGGAGAAGG AGAGAAATTG GTGAGGGCTC TTTTTGCTGT GGCTCGAGAA
    CTTCAACCTT CTATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA
    GGGGAGCACG ATGCTAGTAG ACGCCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA
    CAGTCTGCTG GAGATGACAG AGTACTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT
    GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTACC AAATGAGGAG
    ACAAGACTAC TTTTGCTTAA AAATCTGTTA TGTAAACAAG GAAGTCCATT GACCCAAAAA
    GAACTAGCAC AACTTGCTAG AATGACTGAT GGATACTCAG GAAGTGACCT AACAGCTTTG
    GCAAAAGATG CAGCACTGGG TCCTATCCGA GAACTAAAAC CAGAACAGGT GAAGAATATG
    TCTGCCAGTG AGATGAGAAA TATTCGATTA TCTGACTTCA CTGAATCCTT GAAAAAAATA
    AAACGCAGCG TCAGCCCTCA AACTTTAGAA GCGTACATAC GTTGGAACAA GGACTTTGGA
    GATACCACTG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_955468.1
    CDS222..1976
    Translation

    Target ORF information:

    RefSeq Version NM_199436.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spastin (SPAST), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_199436.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA
    CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA
    GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA
    ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAAAG TGGAGCTGTT
    CCAAAAAGAA AAGACCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT
    ATGAAAACTG GATCTGCAGG CCTTTCAGGC CACCATAGAG CACCTAGTTA CAGTGGTTTA
    TCCATGGTTT CTGGAGTGAA ACAGGGATCT GGTCCTGCTC CTACCACTCA TAAGGGTACT
    CCGAAAACAA ATAGGACAAA TAAACCTTCT ACCCCTACAA CTGCTACTCG TAAGAAAAAA
    GACTTGAAGA ATTTTAGGAA TGTGGACAGC AACCTTGCTA ACCTTATAAT GAATGAAATT
    GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GTCAAGACTT GGCAAAACAA
    GCATTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA
    GCTCCTGCCA GAGGGCTGTT ACTCTTTGGT CCACCTGGGA ATGGGAAGAC AATGCTGGCT
    AAAGCAGTAG CTGCAGAATC GAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT
    TCAAAATACG TGGGAGAAGG AGAGAAATTG GTGAGGGCTC TTTTTGCTGT GGCTCGAGAA
    CTTCAACCTT CTATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA
    GGGGAGCACG ATGCTAGTAG ACGCCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA
    CAGTCTGCTG GAGATGACAG AGTACTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT
    GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTACC AAATGAGGAG
    ACAAGACTAC TTTTGCTTAA AAATCTGTTA TGTAAACAAG GAAGTCCATT GACCCAAAAA
    GAACTAGCAC AACTTGCTAG AATGACTGAT GGATACTCAG GAAGTGACCT AACAGCTTTG
    GCAAAAGATG CAGCACTGGG TCCTATCCGA GAACTAAAAC CAGAACAGGT GAAGAATATG
    TCTGCCAGTG AGATGAGAAA TATTCGATTA TCTGACTTCA CTGAATCCTT GAAAAAAATA
    AAACGCAGCG TCAGCCCTCA AACTTTAGAA GCGTACATAC GTTGGAACAA GGACTTTGGA
    GATACCACTG TTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35573
    Clone ID Related Accession (Same CDS sequence) XM_005264516.4 , NM_001363823.1 , XM_005264516.5
    Accession Version NM_001363823.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product spastin isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121655.1 and AL121658.4. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, which results in a shorter isoform (3), compared to isoform 1. This variant is also predicted to use an alternate, in-frame, downstream translation initiation site to encode an even shorter isoform. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG
    AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT
    GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG
    ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAGAAGAT GCAACCAGTT
    TTGCCATTTT CCAAGTCACA AACGGACGTC TATAATGACA GTACTAACTT GGCATGCCGC
    AATGGACATC TCCAGTCAGA AAGTGGAGCT GTTCCAAAAA GAAAAGACCC CTTAACACAC
    ACTAGTAATT CACTGCCTCG TTCAAAAACA GTTATGAAAA CTGGATCTGC AGGCCTTTCA
    GGCCACCATA GAGCACCTAG TTACAGTGGT TTATCCATGG TTTCTGGAGT GAAACAGGGA
    TCTGGTCCTG CTCCTACCAC TCATAAGGGT ACTCCGAAAA CAAATAGGAC AAATAAACCT
    TCTACCCCTA CAACTGCTAC TCGTAAGAAA AAAGACTTGA AGAATTTTAG GAATGTGGAC
    AGCAACCTTG CTAACCTTAT AATGAATGAA ATTGTGGACA ATGGAACAGC TGTTAAATTT
    GATGATATAG CTGGTCAAGA CTTGGCAAAA CAAGCATTGC AAGAAATTGT TATTCTTCCT
    TCTCTGAGGC CTGAGTTGTT CACAGGGCTT AGAGCTCCTG CCAGAGGGCT GTTACTCTTT
    GGTCCACCTG GGAATGGGAA GACAATGCTG GCTAAAGCAG TAGCTGCAGA ATCGAATGCA
    ACCTTCTTTA ATATAAGTGC TGCAAGTTTA ACTTCAAAAT ACGTGGGAGA AGGAGAGAAA
    TTGGTGAGGG CTCTTTTTGC TGTGGCTCGA GAACTTCAAC CTTCTATAAT TTTTATAGAT
    GAAGTTGATA GCCTTTTGTG TGAAAGAAGA GAAGGGGAGC ACGATGCTAG TAGACGCCTA
    AAAACTGAAT TTCTAATAGA ATTTGATGGT GTACAGTCTG CTGGAGATGA CAGAGTACTT
    GTAATGGGTG CAACTAATAG GCCACAAGAG CTTGATGAGG CTGTTCTCAG GCGTTTCATC
    AAACGGGTAT ATGTGTCTTT ACCAAATGAG GAGACAAGAC TACTTTTGCT TAAAAATCTG
    TTATGTAAAC AAGGAAGTCC ATTGACCCAA AAAGAACTAG CACAACTTGC TAGAATGACT
    GATGGATACT CAGGAAGTGA CCTAACAGCT TTGGCAAAAG ATGCAGCACT GGGTCCTATC
    CGAGAACTAA AACCAGAACA GGTGAAGAAT ATGTCTGCCA GTGAGATGAG AAATATTCGA
    TTATCTGACT TCACTGAATC CTTGAAAAAA ATAAAACGCA GCGTCAGCCC TCAAACTTTA
    GAAGCGTACA TACGTTGGAA CAAGGACTTT GGAGATACCA CTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001350752.1
    CDS222..2069
    Translation

    Target ORF information:

    RefSeq Version NM_001363823.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spastin (SPAST), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001363823.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG
    AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT
    GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG
    ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAGAAGAT GCAACCAGTT
    TTGCCATTTT CCAAGTCACA AACGGACGTC TATAATGACA GTACTAACTT GGCATGCCGC
    AATGGACATC TCCAGTCAGA AAGTGGAGCT GTTCCAAAAA GAAAAGACCC CTTAACACAC
    ACTAGTAATT CACTGCCTCG TTCAAAAACA GTTATGAAAA CTGGATCTGC AGGCCTTTCA
    GGCCACCATA GAGCACCTAG TTACAGTGGT TTATCCATGG TTTCTGGAGT GAAACAGGGA
    TCTGGTCCTG CTCCTACCAC TCATAAGGGT ACTCCGAAAA CAAATAGGAC AAATAAACCT
    TCTACCCCTA CAACTGCTAC TCGTAAGAAA AAAGACTTGA AGAATTTTAG GAATGTGGAC
    AGCAACCTTG CTAACCTTAT AATGAATGAA ATTGTGGACA ATGGAACAGC TGTTAAATTT
    GATGATATAG CTGGTCAAGA CTTGGCAAAA CAAGCATTGC AAGAAATTGT TATTCTTCCT
    TCTCTGAGGC CTGAGTTGTT CACAGGGCTT AGAGCTCCTG CCAGAGGGCT GTTACTCTTT
    GGTCCACCTG GGAATGGGAA GACAATGCTG GCTAAAGCAG TAGCTGCAGA ATCGAATGCA
    ACCTTCTTTA ATATAAGTGC TGCAAGTTTA ACTTCAAAAT ACGTGGGAGA AGGAGAGAAA
    TTGGTGAGGG CTCTTTTTGC TGTGGCTCGA GAACTTCAAC CTTCTATAAT TTTTATAGAT
    GAAGTTGATA GCCTTTTGTG TGAAAGAAGA GAAGGGGAGC ACGATGCTAG TAGACGCCTA
    AAAACTGAAT TTCTAATAGA ATTTGATGGT GTACAGTCTG CTGGAGATGA CAGAGTACTT
    GTAATGGGTG CAACTAATAG GCCACAAGAG CTTGATGAGG CTGTTCTCAG GCGTTTCATC
    AAACGGGTAT ATGTGTCTTT ACCAAATGAG GAGACAAGAC TACTTTTGCT TAAAAATCTG
    TTATGTAAAC AAGGAAGTCC ATTGACCCAA AAAGAACTAG CACAACTTGC TAGAATGACT
    GATGGATACT CAGGAAGTGA CCTAACAGCT TTGGCAAAAG ATGCAGCACT GGGTCCTATC
    CGAGAACTAA AACCAGAACA GGTGAAGAAT ATGTCTGCCA GTGAGATGAG AAATATTCGA
    TTATCTGACT TCACTGAATC CTTGAAAAAA ATAAAACGCA GCGTCAGCCC TCAAACTTTA
    GAAGCGTACA TACGTTGGAA CAAGGACTTT GGAGATACCA CTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89825
    Clone ID Related Accession (Same CDS sequence) NM_001363875.1 , XM_017004777.1
    Accession Version NM_001363875.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product spastin isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL121655.1 and AL121658.4. On May 30, 2018 this sequence version replaced XM_017004777.2. Transcript Variant: This variant (4) has multiple differences in the coding region, compared to variant 1. It encodes a shorter isoform (4), compared to isoform 1. This variant is also predicted to use an alternate, in-frame, downstream translation initiation site to encode an even shorter isoform. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.206341.1, SRR1803611.127602.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG
    AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT
    GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG
    ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAAAGTGG AGCTGTTCCA
    AAAAGAAAAG ACCCCTTAAC ACACACTAGT AATTCACTGC CTCGTTCAAA AACAGTTATG
    AAAACTGGAT CTGCAGGCCT TTCAGGCCAC CATAGAGCAC CTAGTTACAG TGGTTTATCC
    ATGGTTTCTG GAGTGAAACA GGGATCTGGT CCTGCTCCTA CCACTCATAA GGGTACTCCG
    AAAACAAATA GGACAAATAA ACCTTCTACC CCTACAACTG CTACTCGTAA GAAAAAAGAC
    TTGAAGAATT TTAGGAATGT GGACAGCAAC CTTGCTAACC TTATAATGAA TGAAATTGTG
    GACAATGGAA CAGCTGTTAA ATTTGATGAT ATAGCTGGTC AAGACTTGGC AAAACAAGCA
    TTGCAAGAAA TTGTTATTCT TCCTTCTCTG AGGCCTGAGT TGTTCACAGG GCTTAGAGCT
    CCTGCCAGAG GGCTGTTACT CTTTGGTCCA CCTGGGAATG GGAAGACAAT GCTGGCTAAA
    GCAGTAGCTG CAGAATCGAA TGCAACCTTC TTTAATATAA GTGCTGCAAG TTTAACTTCA
    AAATACGTGG GAGAAGGAGA GAAATTGGTG AGGGCTCTTT TTGCTGTGGC TCGAGAACTT
    CAACCTTCTA TAATTTTTAT AGATGAAGTT GATAGCCTTT TGTGTGAAAG AAGAGAAGGG
    GAGCACGATG CTAGTAGACG CCTAAAAACT GAATTTCTAA TAGAATTTGA TGGTGTACAG
    TCTGCTGGAG ATGACAGAGT ACTTGTAATG GGTGCAACTA ATAGGCCACA AGAGCTTGAT
    GAGGCTGTTC TCAGGCGTTT CATCAAACGG GTATATGTGT CTTTACCAAA TGAGGAGACA
    AGACTACTTT TGCTTAAAAA TCTGTTATGT AAACAAGGAA GTCCATTGAC CCAAAAAGAA
    CTAGCACAAC TTGCTAGAAT GACTGATGGA TACTCAGGAA GTGACCTAAC AGCTTTGGCA
    AAAGATGCAG CACTGGGTCC TATCCGAGAA CTAAAACCAG AACAGGTGAA GAATATGTCT
    GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACTG AATCCTTGAA AAAAATAAAA
    CGCAGCGTCA GCCCTCAAAC TTTAGAAGCG TACATACGTT GGAACAAGGA CTTTGGAGAT
    ACCACTGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001350804.1
    CDS222..1973
    Translation

    Target ORF information:

    RefSeq Version NM_001363875.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spastin (SPAST), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001363875.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG
    AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT
    GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG
    ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAAAGTGG AGCTGTTCCA
    AAAAGAAAAG ACCCCTTAAC ACACACTAGT AATTCACTGC CTCGTTCAAA AACAGTTATG
    AAAACTGGAT CTGCAGGCCT TTCAGGCCAC CATAGAGCAC CTAGTTACAG TGGTTTATCC
    ATGGTTTCTG GAGTGAAACA GGGATCTGGT CCTGCTCCTA CCACTCATAA GGGTACTCCG
    AAAACAAATA GGACAAATAA ACCTTCTACC CCTACAACTG CTACTCGTAA GAAAAAAGAC
    TTGAAGAATT TTAGGAATGT GGACAGCAAC CTTGCTAACC TTATAATGAA TGAAATTGTG
    GACAATGGAA CAGCTGTTAA ATTTGATGAT ATAGCTGGTC AAGACTTGGC AAAACAAGCA
    TTGCAAGAAA TTGTTATTCT TCCTTCTCTG AGGCCTGAGT TGTTCACAGG GCTTAGAGCT
    CCTGCCAGAG GGCTGTTACT CTTTGGTCCA CCTGGGAATG GGAAGACAAT GCTGGCTAAA
    GCAGTAGCTG CAGAATCGAA TGCAACCTTC TTTAATATAA GTGCTGCAAG TTTAACTTCA
    AAATACGTGG GAGAAGGAGA GAAATTGGTG AGGGCTCTTT TTGCTGTGGC TCGAGAACTT
    CAACCTTCTA TAATTTTTAT AGATGAAGTT GATAGCCTTT TGTGTGAAAG AAGAGAAGGG
    GAGCACGATG CTAGTAGACG CCTAAAAACT GAATTTCTAA TAGAATTTGA TGGTGTACAG
    TCTGCTGGAG ATGACAGAGT ACTTGTAATG GGTGCAACTA ATAGGCCACA AGAGCTTGAT
    GAGGCTGTTC TCAGGCGTTT CATCAAACGG GTATATGTGT CTTTACCAAA TGAGGAGACA
    AGACTACTTT TGCTTAAAAA TCTGTTATGT AAACAAGGAA GTCCATTGAC CCAAAAAGAA
    CTAGCACAAC TTGCTAGAAT GACTGATGGA TACTCAGGAA GTGACCTAAC AGCTTTGGCA
    AAAGATGCAG CACTGGGTCC TATCCGAGAA CTAAAACCAG AACAGGTGAA GAATATGTCT
    GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACTG AATCCTTGAA AAAAATAAAA
    CGCAGCGTCA GCCCTCAAAC TTTAGAAGCG TACATACGTT GGAACAAGGA CTTTGGAGAT
    ACCACTGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35573
    Clone ID Related Accession (Same CDS sequence) XM_005264516.4 , NM_001363823.1 , XM_005264516.5
    Accession Version XM_005264516.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spastin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005264516.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG
    AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT
    GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG
    ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAGAAGAT GCAACCAGTT
    TTGCCATTTT CCAAGTCACA AACGGACGTC TATAATGACA GTACTAACTT GGCATGCCGC
    AATGGACATC TCCAGTCAGA AAGTGGAGCT GTTCCAAAAA GAAAAGACCC CTTAACACAC
    ACTAGTAATT CACTGCCTCG TTCAAAAACA GTTATGAAAA CTGGATCTGC AGGCCTTTCA
    GGCCACCATA GAGCACCTAG TTACAGTGGT TTATCCATGG TTTCTGGAGT GAAACAGGGA
    TCTGGTCCTG CTCCTACCAC TCATAAGGGT ACTCCGAAAA CAAATAGGAC AAATAAACCT
    TCTACCCCTA CAACTGCTAC TCGTAAGAAA AAAGACTTGA AGAATTTTAG GAATGTGGAC
    AGCAACCTTG CTAACCTTAT AATGAATGAA ATTGTGGACA ATGGAACAGC TGTTAAATTT
    GATGATATAG CTGGTCAAGA CTTGGCAAAA CAAGCATTGC AAGAAATTGT TATTCTTCCT
    TCTCTGAGGC CTGAGTTGTT CACAGGGCTT AGAGCTCCTG CCAGAGGGCT GTTACTCTTT
    GGTCCACCTG GGAATGGGAA GACAATGCTG GCTAAAGCAG TAGCTGCAGA ATCGAATGCA
    ACCTTCTTTA ATATAAGTGC TGCAAGTTTA ACTTCAAAAT ACGTGGGAGA AGGAGAGAAA
    TTGGTGAGGG CTCTTTTTGC TGTGGCTCGA GAACTTCAAC CTTCTATAAT TTTTATAGAT
    GAAGTTGATA GCCTTTTGTG TGAAAGAAGA GAAGGGGAGC ACGATGCTAG TAGACGCCTA
    AAAACTGAAT TTCTAATAGA ATTTGATGGT GTACAGTCTG CTGGAGATGA CAGAGTACTT
    GTAATGGGTG CAACTAATAG GCCACAAGAG CTTGATGAGG CTGTTCTCAG GCGTTTCATC
    AAACGGGTAT ATGTGTCTTT ACCAAATGAG GAGACAAGAC TACTTTTGCT TAAAAATCTG
    TTATGTAAAC AAGGAAGTCC ATTGACCCAA AAAGAACTAG CACAACTTGC TAGAATGACT
    GATGGATACT CAGGAAGTGA CCTAACAGCT TTGGCAAAAG ATGCAGCACT GGGTCCTATC
    CGAGAACTAA AACCAGAACA GGTGAAGAAT ATGTCTGCCA GTGAGATGAG AAATATTCGA
    TTATCTGACT TCACTGAATC CTTGAAAAAA ATAAAACGCA GCGTCAGCCC TCAAACTTTA
    GAAGCGTACA TACGTTGGAA CAAGGACTTT GGAGATACCA CTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264573.1
    CDS1..1848
    Translation

    Target ORF information:

    RefSeq Version XM_005264516.4
    Organism Homo sapiens(human)
    Definition Homo sapiens spastin (SPAST), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264516.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG
    AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT
    GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG
    ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAGAAGAT GCAACCAGTT
    TTGCCATTTT CCAAGTCACA AACGGACGTC TATAATGACA GTACTAACTT GGCATGCCGC
    AATGGACATC TCCAGTCAGA AAGTGGAGCT GTTCCAAAAA GAAAAGACCC CTTAACACAC
    ACTAGTAATT CACTGCCTCG TTCAAAAACA GTTATGAAAA CTGGATCTGC AGGCCTTTCA
    GGCCACCATA GAGCACCTAG TTACAGTGGT TTATCCATGG TTTCTGGAGT GAAACAGGGA
    TCTGGTCCTG CTCCTACCAC TCATAAGGGT ACTCCGAAAA CAAATAGGAC AAATAAACCT
    TCTACCCCTA CAACTGCTAC TCGTAAGAAA AAAGACTTGA AGAATTTTAG GAATGTGGAC
    AGCAACCTTG CTAACCTTAT AATGAATGAA ATTGTGGACA ATGGAACAGC TGTTAAATTT
    GATGATATAG CTGGTCAAGA CTTGGCAAAA CAAGCATTGC AAGAAATTGT TATTCTTCCT
    TCTCTGAGGC CTGAGTTGTT CACAGGGCTT AGAGCTCCTG CCAGAGGGCT GTTACTCTTT
    GGTCCACCTG GGAATGGGAA GACAATGCTG GCTAAAGCAG TAGCTGCAGA ATCGAATGCA
    ACCTTCTTTA ATATAAGTGC TGCAAGTTTA ACTTCAAAAT ACGTGGGAGA AGGAGAGAAA
    TTGGTGAGGG CTCTTTTTGC TGTGGCTCGA GAACTTCAAC CTTCTATAAT TTTTATAGAT
    GAAGTTGATA GCCTTTTGTG TGAAAGAAGA GAAGGGGAGC ACGATGCTAG TAGACGCCTA
    AAAACTGAAT TTCTAATAGA ATTTGATGGT GTACAGTCTG CTGGAGATGA CAGAGTACTT
    GTAATGGGTG CAACTAATAG GCCACAAGAG CTTGATGAGG CTGTTCTCAG GCGTTTCATC
    AAACGGGTAT ATGTGTCTTT ACCAAATGAG GAGACAAGAC TACTTTTGCT TAAAAATCTG
    TTATGTAAAC AAGGAAGTCC ATTGACCCAA AAAGAACTAG CACAACTTGC TAGAATGACT
    GATGGATACT CAGGAAGTGA CCTAACAGCT TTGGCAAAAG ATGCAGCACT GGGTCCTATC
    CGAGAACTAA AACCAGAACA GGTGAAGAAT ATGTCTGCCA GTGAGATGAG AAATATTCGA
    TTATCTGACT TCACTGAATC CTTGAAAAAA ATAAAACGCA GCGTCAGCCC TCAAACTTTA
    GAAGCGTACA TACGTTGGAA CAAGGACTTT GGAGATACCA CTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu35573
    Clone ID Related Accession (Same CDS sequence) XM_005264516.4 , NM_001363823.1 , XM_005264516.5
    Accession Version XM_005264516.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1848bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product spastin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005264516.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG
    AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT
    GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG
    ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAGAAGAT GCAACCAGTT
    TTGCCATTTT CCAAGTCACA AACGGACGTC TATAATGACA GTACTAACTT GGCATGCCGC
    AATGGACATC TCCAGTCAGA AAGTGGAGCT GTTCCAAAAA GAAAAGACCC CTTAACACAC
    ACTAGTAATT CACTGCCTCG TTCAAAAACA GTTATGAAAA CTGGATCTGC AGGCCTTTCA
    GGCCACCATA GAGCACCTAG TTACAGTGGT TTATCCATGG TTTCTGGAGT GAAACAGGGA
    TCTGGTCCTG CTCCTACCAC TCATAAGGGT ACTCCGAAAA CAAATAGGAC AAATAAACCT
    TCTACCCCTA CAACTGCTAC TCGTAAGAAA AAAGACTTGA AGAATTTTAG GAATGTGGAC
    AGCAACCTTG CTAACCTTAT AATGAATGAA ATTGTGGACA ATGGAACAGC TGTTAAATTT
    GATGATATAG CTGGTCAAGA CTTGGCAAAA CAAGCATTGC AAGAAATTGT TATTCTTCCT
    TCTCTGAGGC CTGAGTTGTT CACAGGGCTT AGAGCTCCTG CCAGAGGGCT GTTACTCTTT
    GGTCCACCTG GGAATGGGAA GACAATGCTG GCTAAAGCAG TAGCTGCAGA ATCGAATGCA
    ACCTTCTTTA ATATAAGTGC TGCAAGTTTA ACTTCAAAAT ACGTGGGAGA AGGAGAGAAA
    TTGGTGAGGG CTCTTTTTGC TGTGGCTCGA GAACTTCAAC CTTCTATAAT TTTTATAGAT
    GAAGTTGATA GCCTTTTGTG TGAAAGAAGA GAAGGGGAGC ACGATGCTAG TAGACGCCTA
    AAAACTGAAT TTCTAATAGA ATTTGATGGT GTACAGTCTG CTGGAGATGA CAGAGTACTT
    GTAATGGGTG CAACTAATAG GCCACAAGAG CTTGATGAGG CTGTTCTCAG GCGTTTCATC
    AAACGGGTAT ATGTGTCTTT ACCAAATGAG GAGACAAGAC TACTTTTGCT TAAAAATCTG
    TTATGTAAAC AAGGAAGTCC ATTGACCCAA AAAGAACTAG CACAACTTGC TAGAATGACT
    GATGGATACT CAGGAAGTGA CCTAACAGCT TTGGCAAAAG ATGCAGCACT GGGTCCTATC
    CGAGAACTAA AACCAGAACA GGTGAAGAAT ATGTCTGCCA GTGAGATGAG AAATATTCGA
    TTATCTGACT TCACTGAATC CTTGAAAAAA ATAAAACGCA GCGTCAGCCC TCAAACTTTA
    GAAGCGTACA TACGTTGGAA CAAGGACTTT GGAGATACCA CTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005264573.1
    CDS1..1848
    Translation

    Target ORF information:

    RefSeq Version XM_005264516.5
    Organism Homo sapiens(human)
    Definition Homo sapiens spastin (SPAST), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005264516.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG
    AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT
    GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG
    ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAGAAGAT GCAACCAGTT
    TTGCCATTTT CCAAGTCACA AACGGACGTC TATAATGACA GTACTAACTT GGCATGCCGC
    AATGGACATC TCCAGTCAGA AAGTGGAGCT GTTCCAAAAA GAAAAGACCC CTTAACACAC
    ACTAGTAATT CACTGCCTCG TTCAAAAACA GTTATGAAAA CTGGATCTGC AGGCCTTTCA
    GGCCACCATA GAGCACCTAG TTACAGTGGT TTATCCATGG TTTCTGGAGT GAAACAGGGA
    TCTGGTCCTG CTCCTACCAC TCATAAGGGT ACTCCGAAAA CAAATAGGAC AAATAAACCT
    TCTACCCCTA CAACTGCTAC TCGTAAGAAA AAAGACTTGA AGAATTTTAG GAATGTGGAC
    AGCAACCTTG CTAACCTTAT AATGAATGAA ATTGTGGACA ATGGAACAGC TGTTAAATTT
    GATGATATAG CTGGTCAAGA CTTGGCAAAA CAAGCATTGC AAGAAATTGT TATTCTTCCT
    TCTCTGAGGC CTGAGTTGTT CACAGGGCTT AGAGCTCCTG CCAGAGGGCT GTTACTCTTT
    GGTCCACCTG GGAATGGGAA GACAATGCTG GCTAAAGCAG TAGCTGCAGA ATCGAATGCA
    ACCTTCTTTA ATATAAGTGC TGCAAGTTTA ACTTCAAAAT ACGTGGGAGA AGGAGAGAAA
    TTGGTGAGGG CTCTTTTTGC TGTGGCTCGA GAACTTCAAC CTTCTATAAT TTTTATAGAT
    GAAGTTGATA GCCTTTTGTG TGAAAGAAGA GAAGGGGAGC ACGATGCTAG TAGACGCCTA
    AAAACTGAAT TTCTAATAGA ATTTGATGGT GTACAGTCTG CTGGAGATGA CAGAGTACTT
    GTAATGGGTG CAACTAATAG GCCACAAGAG CTTGATGAGG CTGTTCTCAG GCGTTTCATC
    AAACGGGTAT ATGTGTCTTT ACCAAATGAG GAGACAAGAC TACTTTTGCT TAAAAATCTG
    TTATGTAAAC AAGGAAGTCC ATTGACCCAA AAAGAACTAG CACAACTTGC TAGAATGACT
    GATGGATACT CAGGAAGTGA CCTAACAGCT TTGGCAAAAG ATGCAGCACT GGGTCCTATC
    CGAGAACTAA AACCAGAACA GGTGAAGAAT ATGTCTGCCA GTGAGATGAG AAATATTCGA
    TTATCTGACT TCACTGAATC CTTGAAAAAA ATAAAACGCA GCGTCAGCCC TCAAACTTTA
    GAAGCGTACA TACGTTGGAA CAAGGACTTT GGAGATACCA CTGTTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu60708
    Clone ID Related Accession (Same CDS sequence) XM_011533067.2
    Accession Version XM_011533067.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1656bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spastin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533067.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA
    CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA
    GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA
    ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAGAA GATGCAACCA
    GTTTTGCCAT TTTCCAAGTC ACAAACGGAC GTCTATAATG ACAGTACTAA CTTGGCATGC
    CGCAATGGAC ATCTCCAGTC AGAAAGTGGA GCTGTTCCAA AAAGAAAAGA CCCCTTAACA
    CACACTAGTA ATTCACTGCC TCGTTCAAAA ACAGTTATGA AAACTGGATC TGCAGGCCTT
    TCAGGCCACC ATAGAGCACC TAGTTACAGT GGTTTATCCA TGGTTTCTGG AGTGAAACAG
    GGATCTGGTC CTGCTCCTAC CACTCATAAG GGTACTCCGA AAACAAATAG GACAAATAAA
    CCTTCTACCC CTACAACTGC TACTCGTAAG AAAAAAGACT TGAAGAATTT TAGGAATGTG
    GACAGCAACC TTGCTAACCT TATAATGAAT GAAATTGTGG ACAATGGAAC AGCTGTTAAA
    TTTGATGATA TAGCTGGTCA AGACTTGGCA AAACAAGCAT TGCAAGAAAT TGTTATTCTT
    CCTTCTCTGA GGCCTGAGTT GTTCACAGGG CTTAGAGCTC CTGCCAGAGG GCTGTTACTC
    TTTGGTCCAC CTGGGAATGG GAAGACAATG CTGGCTAAAG CAGTAGCTGC AGAATCGAAT
    GCAACCTTCT TTAATATAAG TGCTGCAAGT TTAACTTCAA AATACGTGGG AGAAGGAGAG
    AAATTGGTGA GGGCTCTTTT TGCTGTGGCT CGAGAACTTC AACCTTCTAT AATTTTTATA
    GATGAAGTTG ATAGCCTTTT GTGTGAAAGA AGAGAAGGGG AGCACGATGC TAGTAGACGC
    CTAAAAACTG AATTTCTAAT AGAATTTGAT GGTGTACAGT CTGCTGGAGA TGACAGAGTA
    CTTGTAATGG GTGCAACTAA TAGGCCACAA GAGCTTGATG AGGCTGTTCT CAGGCGTTTC
    ATCAAACGGG TATATGTGTC TTTACCAAAT GAGGAGACAA GACTACTTTT GCTTAAAAAT
    CTGTTATGTA AACAAGGAAG TCCATTGACC CAAAAAGAAC TAGCACAACT TGCTAGAACT
    AAAACCAGAA CAGGTGAAGA ATATGTCTGC CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011531369.1
    CDS282..1937
    Translation

    Target ORF information:

    RefSeq Version XM_011533067.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spastin (SPAST), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011533067.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA
    CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA
    GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA
    ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAGAA GATGCAACCA
    GTTTTGCCAT TTTCCAAGTC ACAAACGGAC GTCTATAATG ACAGTACTAA CTTGGCATGC
    CGCAATGGAC ATCTCCAGTC AGAAAGTGGA GCTGTTCCAA AAAGAAAAGA CCCCTTAACA
    CACACTAGTA ATTCACTGCC TCGTTCAAAA ACAGTTATGA AAACTGGATC TGCAGGCCTT
    TCAGGCCACC ATAGAGCACC TAGTTACAGT GGTTTATCCA TGGTTTCTGG AGTGAAACAG
    GGATCTGGTC CTGCTCCTAC CACTCATAAG GGTACTCCGA AAACAAATAG GACAAATAAA
    CCTTCTACCC CTACAACTGC TACTCGTAAG AAAAAAGACT TGAAGAATTT TAGGAATGTG
    GACAGCAACC TTGCTAACCT TATAATGAAT GAAATTGTGG ACAATGGAAC AGCTGTTAAA
    TTTGATGATA TAGCTGGTCA AGACTTGGCA AAACAAGCAT TGCAAGAAAT TGTTATTCTT
    CCTTCTCTGA GGCCTGAGTT GTTCACAGGG CTTAGAGCTC CTGCCAGAGG GCTGTTACTC
    TTTGGTCCAC CTGGGAATGG GAAGACAATG CTGGCTAAAG CAGTAGCTGC AGAATCGAAT
    GCAACCTTCT TTAATATAAG TGCTGCAAGT TTAACTTCAA AATACGTGGG AGAAGGAGAG
    AAATTGGTGA GGGCTCTTTT TGCTGTGGCT CGAGAACTTC AACCTTCTAT AATTTTTATA
    GATGAAGTTG ATAGCCTTTT GTGTGAAAGA AGAGAAGGGG AGCACGATGC TAGTAGACGC
    CTAAAAACTG AATTTCTAAT AGAATTTGAT GGTGTACAGT CTGCTGGAGA TGACAGAGTA
    CTTGTAATGG GTGCAACTAA TAGGCCACAA GAGCTTGATG AGGCTGTTCT CAGGCGTTTC
    ATCAAACGGG TATATGTGTC TTTACCAAAT GAGGAGACAA GACTACTTTT GCTTAAAAAT
    CTGTTATGTA AACAAGGAAG TCCATTGACC CAAAAAGAAC TAGCACAACT TGCTAGAACT
    AAAACCAGAA CAGGTGAAGA ATATGTCTGC CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89825
    Clone ID Related Accession (Same CDS sequence) NM_001363875.1 , XM_017004777.1
    Accession Version XM_017004777.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spastin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG
    AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT
    GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG
    ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAAAGTGG AGCTGTTCCA
    AAAAGAAAAG ACCCCTTAAC ACACACTAGT AATTCACTGC CTCGTTCAAA AACAGTTATG
    AAAACTGGAT CTGCAGGCCT TTCAGGCCAC CATAGAGCAC CTAGTTACAG TGGTTTATCC
    ATGGTTTCTG GAGTGAAACA GGGATCTGGT CCTGCTCCTA CCACTCATAA GGGTACTCCG
    AAAACAAATA GGACAAATAA ACCTTCTACC CCTACAACTG CTACTCGTAA GAAAAAAGAC
    TTGAAGAATT TTAGGAATGT GGACAGCAAC CTTGCTAACC TTATAATGAA TGAAATTGTG
    GACAATGGAA CAGCTGTTAA ATTTGATGAT ATAGCTGGTC AAGACTTGGC AAAACAAGCA
    TTGCAAGAAA TTGTTATTCT TCCTTCTCTG AGGCCTGAGT TGTTCACAGG GCTTAGAGCT
    CCTGCCAGAG GGCTGTTACT CTTTGGTCCA CCTGGGAATG GGAAGACAAT GCTGGCTAAA
    GCAGTAGCTG CAGAATCGAA TGCAACCTTC TTTAATATAA GTGCTGCAAG TTTAACTTCA
    AAATACGTGG GAGAAGGAGA GAAATTGGTG AGGGCTCTTT TTGCTGTGGC TCGAGAACTT
    CAACCTTCTA TAATTTTTAT AGATGAAGTT GATAGCCTTT TGTGTGAAAG AAGAGAAGGG
    GAGCACGATG CTAGTAGACG CCTAAAAACT GAATTTCTAA TAGAATTTGA TGGTGTACAG
    TCTGCTGGAG ATGACAGAGT ACTTGTAATG GGTGCAACTA ATAGGCCACA AGAGCTTGAT
    GAGGCTGTTC TCAGGCGTTT CATCAAACGG GTATATGTGT CTTTACCAAA TGAGGAGACA
    AGACTACTTT TGCTTAAAAA TCTGTTATGT AAACAAGGAA GTCCATTGAC CCAAAAAGAA
    CTAGCACAAC TTGCTAGAAT GACTGATGGA TACTCAGGAA GTGACCTAAC AGCTTTGGCA
    AAAGATGCAG CACTGGGTCC TATCCGAGAA CTAAAACCAG AACAGGTGAA GAATATGTCT
    GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACTG AATCCTTGAA AAAAATAAAA
    CGCAGCGTCA GCCCTCAAAC TTTAGAAGCG TACATACGTT GGAACAAGGA CTTTGGAGAT
    ACCACTGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860266.1
    CDS282..2033
    Translation

    Target ORF information:

    RefSeq Version XM_017004777.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spastin (SPAST), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004777.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGGACAG
    AAGGAGCAAG CTGTGGAATG GTATAAGAAA GGTATTGAAG AACTGGAAAA AGGAATAGCT
    GTTATAGTTA CAGGACAAGG TGAACAGTGT GAAAGAGCTA GACGCCTTCA AGCTAAAATG
    ATGACTAATT TGGTTATGGC CAAGGACCGC TTACAACTTC TAGAAAGTGG AGCTGTTCCA
    AAAAGAAAAG ACCCCTTAAC ACACACTAGT AATTCACTGC CTCGTTCAAA AACAGTTATG
    AAAACTGGAT CTGCAGGCCT TTCAGGCCAC CATAGAGCAC CTAGTTACAG TGGTTTATCC
    ATGGTTTCTG GAGTGAAACA GGGATCTGGT CCTGCTCCTA CCACTCATAA GGGTACTCCG
    AAAACAAATA GGACAAATAA ACCTTCTACC CCTACAACTG CTACTCGTAA GAAAAAAGAC
    TTGAAGAATT TTAGGAATGT GGACAGCAAC CTTGCTAACC TTATAATGAA TGAAATTGTG
    GACAATGGAA CAGCTGTTAA ATTTGATGAT ATAGCTGGTC AAGACTTGGC AAAACAAGCA
    TTGCAAGAAA TTGTTATTCT TCCTTCTCTG AGGCCTGAGT TGTTCACAGG GCTTAGAGCT
    CCTGCCAGAG GGCTGTTACT CTTTGGTCCA CCTGGGAATG GGAAGACAAT GCTGGCTAAA
    GCAGTAGCTG CAGAATCGAA TGCAACCTTC TTTAATATAA GTGCTGCAAG TTTAACTTCA
    AAATACGTGG GAGAAGGAGA GAAATTGGTG AGGGCTCTTT TTGCTGTGGC TCGAGAACTT
    CAACCTTCTA TAATTTTTAT AGATGAAGTT GATAGCCTTT TGTGTGAAAG AAGAGAAGGG
    GAGCACGATG CTAGTAGACG CCTAAAAACT GAATTTCTAA TAGAATTTGA TGGTGTACAG
    TCTGCTGGAG ATGACAGAGT ACTTGTAATG GGTGCAACTA ATAGGCCACA AGAGCTTGAT
    GAGGCTGTTC TCAGGCGTTT CATCAAACGG GTATATGTGT CTTTACCAAA TGAGGAGACA
    AGACTACTTT TGCTTAAAAA TCTGTTATGT AAACAAGGAA GTCCATTGAC CCAAAAAGAA
    CTAGCACAAC TTGCTAGAAT GACTGATGGA TACTCAGGAA GTGACCTAAC AGCTTTGGCA
    AAAGATGCAG CACTGGGTCC TATCCGAGAA CTAAAACCAG AACAGGTGAA GAATATGTCT
    GCCAGTGAGA TGAGAAATAT TCGATTATCT GACTTCACTG AATCCTTGAA AAAAATAAAA
    CGCAGCGTCA GCCCTCAAAC TTTAGAAGCG TACATACGTT GGAACAAGGA CTTTGGAGAT
    ACCACTGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89826
    Clone ID Related Accession (Same CDS sequence) XM_017004778.1 , XM_017004778.2
    Accession Version XM_017004778.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product spastin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA
    CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA
    GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA
    ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAAAG TGGAGCTGTT
    CCAAAAAGAA AAGACCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT
    ATGAAAACTG GATCTGCAGG CCTTTCAGGC CACCATAGAG CACCTAGTTA CAGTGGTTTA
    TCCATGGTTT CTGGAGTGAA ACAGGGATCT GGTCCTGCTC CTACCACTCA TAAGGGTACT
    CCGAAAACAA ATAGGACAAA TAAACCTTCT ACCCCTACAA CTGCTACTCG TAAGAAAAAA
    GACTTGAAGA ATTTTAGGAA TGTGGACAGC AACCTTGCTA ACCTTATAAT GAATGAAATT
    GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GTCAAGACTT GGCAAAACAA
    GCATTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA
    GCTCCTGCCA GAGGGCTGTT ACTCTTTGGT CCACCTGGGA ATGGGAAGAC AATGCTGGCT
    AAAGCAGTAG CTGCAGAATC GAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT
    TCAAAATACG TGGGAGAAGG AGAGAAATTG GTGAGGGCTC TTTTTGCTGT GGCTCGAGAA
    CTTCAACCTT CTATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA
    GGGGAGCACG ATGCTAGTAG ACGCCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA
    CAGTCTGCTG GAGATGACAG AGTACTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT
    GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTACC AAATGAGGAG
    ACAAGACTAC TTTTGCTTAA AAATCTGTTA TGTAAACAAG GAAGTCCATT GACCCAAAAA
    GAACTAGCAC AACTTGCTAG AACTAAAACC AGAACAGGTG AAGAATATGT CTGCCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860267.1
    CDS282..1841
    Translation

    Target ORF information:

    RefSeq Version XM_017004778.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spastin (SPAST), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004778.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA
    CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA
    GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA
    ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAAAG TGGAGCTGTT
    CCAAAAAGAA AAGACCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT
    ATGAAAACTG GATCTGCAGG CCTTTCAGGC CACCATAGAG CACCTAGTTA CAGTGGTTTA
    TCCATGGTTT CTGGAGTGAA ACAGGGATCT GGTCCTGCTC CTACCACTCA TAAGGGTACT
    CCGAAAACAA ATAGGACAAA TAAACCTTCT ACCCCTACAA CTGCTACTCG TAAGAAAAAA
    GACTTGAAGA ATTTTAGGAA TGTGGACAGC AACCTTGCTA ACCTTATAAT GAATGAAATT
    GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GTCAAGACTT GGCAAAACAA
    GCATTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA
    GCTCCTGCCA GAGGGCTGTT ACTCTTTGGT CCACCTGGGA ATGGGAAGAC AATGCTGGCT
    AAAGCAGTAG CTGCAGAATC GAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT
    TCAAAATACG TGGGAGAAGG AGAGAAATTG GTGAGGGCTC TTTTTGCTGT GGCTCGAGAA
    CTTCAACCTT CTATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA
    GGGGAGCACG ATGCTAGTAG ACGCCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA
    CAGTCTGCTG GAGATGACAG AGTACTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT
    GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTACC AAATGAGGAG
    ACAAGACTAC TTTTGCTTAA AAATCTGTTA TGTAAACAAG GAAGTCCATT GACCCAAAAA
    GAACTAGCAC AACTTGCTAG AACTAAAACC AGAACAGGTG AAGAATATGT CTGCCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89826
    Clone ID Related Accession (Same CDS sequence) XM_017004778.1 , XM_017004778.2
    Accession Version XM_017004778.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1560bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spastin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017004778.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA
    CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA
    GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA
    ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAAAG TGGAGCTGTT
    CCAAAAAGAA AAGACCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT
    ATGAAAACTG GATCTGCAGG CCTTTCAGGC CACCATAGAG CACCTAGTTA CAGTGGTTTA
    TCCATGGTTT CTGGAGTGAA ACAGGGATCT GGTCCTGCTC CTACCACTCA TAAGGGTACT
    CCGAAAACAA ATAGGACAAA TAAACCTTCT ACCCCTACAA CTGCTACTCG TAAGAAAAAA
    GACTTGAAGA ATTTTAGGAA TGTGGACAGC AACCTTGCTA ACCTTATAAT GAATGAAATT
    GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GTCAAGACTT GGCAAAACAA
    GCATTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA
    GCTCCTGCCA GAGGGCTGTT ACTCTTTGGT CCACCTGGGA ATGGGAAGAC AATGCTGGCT
    AAAGCAGTAG CTGCAGAATC GAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT
    TCAAAATACG TGGGAGAAGG AGAGAAATTG GTGAGGGCTC TTTTTGCTGT GGCTCGAGAA
    CTTCAACCTT CTATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA
    GGGGAGCACG ATGCTAGTAG ACGCCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA
    CAGTCTGCTG GAGATGACAG AGTACTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT
    GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTACC AAATGAGGAG
    ACAAGACTAC TTTTGCTTAA AAATCTGTTA TGTAAACAAG GAAGTCCATT GACCCAAAAA
    GAACTAGCAC AACTTGCTAG AACTAAAACC AGAACAGGTG AAGAATATGT CTGCCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860267.1
    CDS282..1841
    Translation

    Target ORF information:

    RefSeq Version XM_017004778.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spastin (SPAST), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004778.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGAATTCTC CGGGTGGACG AGGGAAGAAG AAAGGCTCCG GCGGCGCCAG CAACCCGGTG 
    CCTCCCAGGC CTCCGCCCCC TTGCCTGGCC CCCGCCCCTC CCGCCGCCGG GCCGGCCCCT
    CCGCCCGAGT CGCCGCATAA GCGGAACCTG TACTATTTCT CCTACCCGCT GTTTGTAGGC
    TTCGCGCTGC TGCGTTTGGT CGCCTTCCAC CTGGGGCTCC TCTTCGTGTG GCTCTGCCAG
    CGCTTCTCCC GCGCCCTCAT GGCAGCCAAG AGGAGCTCCG GGGCCGCGCC AGCACCTGCC
    TCGGCCTCGG CCCCGGCGCC GGTGCCGGGC GGCGAGGCCG AGCGCGTCCG AGTCTTCCAC
    AAACAGGCCT TCGAGTACAT CTCCATTGCC CTGCGCATCG ATGAGGATGA GAAAGCAGGA
    CAGAAGGAGC AAGCTGTGGA ATGGTATAAG AAAGGTATTG AAGAACTGGA AAAAGGAATA
    GCTGTTATAG TTACAGGACA AGGTGAACAG TGTGAAAGAG CTAGACGCCT TCAAGCTAAA
    ATGATGACTA ATTTGGTTAT GGCCAAGGAC CGCTTACAAC TTCTAGAAAG TGGAGCTGTT
    CCAAAAAGAA AAGACCCCTT AACACACACT AGTAATTCAC TGCCTCGTTC AAAAACAGTT
    ATGAAAACTG GATCTGCAGG CCTTTCAGGC CACCATAGAG CACCTAGTTA CAGTGGTTTA
    TCCATGGTTT CTGGAGTGAA ACAGGGATCT GGTCCTGCTC CTACCACTCA TAAGGGTACT
    CCGAAAACAA ATAGGACAAA TAAACCTTCT ACCCCTACAA CTGCTACTCG TAAGAAAAAA
    GACTTGAAGA ATTTTAGGAA TGTGGACAGC AACCTTGCTA ACCTTATAAT GAATGAAATT
    GTGGACAATG GAACAGCTGT TAAATTTGAT GATATAGCTG GTCAAGACTT GGCAAAACAA
    GCATTGCAAG AAATTGTTAT TCTTCCTTCT CTGAGGCCTG AGTTGTTCAC AGGGCTTAGA
    GCTCCTGCCA GAGGGCTGTT ACTCTTTGGT CCACCTGGGA ATGGGAAGAC AATGCTGGCT
    AAAGCAGTAG CTGCAGAATC GAATGCAACC TTCTTTAATA TAAGTGCTGC AAGTTTAACT
    TCAAAATACG TGGGAGAAGG AGAGAAATTG GTGAGGGCTC TTTTTGCTGT GGCTCGAGAA
    CTTCAACCTT CTATAATTTT TATAGATGAA GTTGATAGCC TTTTGTGTGA AAGAAGAGAA
    GGGGAGCACG ATGCTAGTAG ACGCCTAAAA ACTGAATTTC TAATAGAATT TGATGGTGTA
    CAGTCTGCTG GAGATGACAG AGTACTTGTA ATGGGTGCAA CTAATAGGCC ACAAGAGCTT
    GATGAGGCTG TTCTCAGGCG TTTCATCAAA CGGGTATATG TGTCTTTACC AAATGAGGAG
    ACAAGACTAC TTTTGCTTAA AAATCTGTTA TGTAAACAAG GAAGTCCATT GACCCAAAAA
    GAACTAGCAC AACTTGCTAG AACTAAAACC AGAACAGGTG AAGAATATGT CTGCCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.