SLC12A3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC12A3
Entrez Gene ID	6559
Full Name	solute carrier family 12 member 3
Synonyms	NCC,NCCT,TSC
General protein information	Preferred Names solute carrier family 12 member 3 Names solute carrier family 12 member 3 Na-Cl cotransporter Na-Cl symporter NaCl electroneutral thiazide-sensitive cotransporter solute carrier family 12 (sodium/chloride transporter), member 3 thiazide-sensitive Na-Cl cotransporter thiazide-sensitive sodium-chloride cotransporter
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q13
Summary	This gene encodes a renal thiazide-sensitive sodium-chloride cotransporter that is important for electrolyte homeostasis. This cotransporter mediates sodium and chloride reabsorption in the distal convoluted tubule. Mutations in this gene cause Gitelman syndrome, a disease similar to Bartter's syndrome, that is characterized by hypokalemic alkalosis combined with hypomagnesemia, low urinary calcium, and increased renin activity associated with normal blood pressure. This cotransporter is the target for thiazide diuretics that are used for treating high blood pressure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008].
Disorder MIM:	600968
Disorder Html:	Gitelman syndrome, 263800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001126107.1	NP_001119579.1	solute carrier family 12 member 3 isoform 2
NM_001126108.2	NP_001119580.2	solute carrier family 12 member 3 isoform 3
XM_005256119.2	XP_005256176.1	solute carrier family 12 member 3 isoform X1
NM_001126108.1	NP_001119580.1	solute carrier family 12 member 3 isoform 3
NM_000339.2	NP_000330.2	solute carrier family 12 member 3 isoform 1
NM_000339.3	NP_000330.3	solute carrier family 12 member 3 isoform 1
NM_001126107.2	NP_001119579.2	solute carrier family 12 member 3 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Slc12a3	NP_062288.2
Rattus norvegicus (Norway rat)	Slc12a3	NP_062218.3
Xenopus tropicalis (tropical clawed frog)	slc12a3	XP_002937217.2
Gallus gallus (chicken)	SLC12A3	XP_414059.4
	SLC12A3	XP_001093467.2
Canis lupus familiaris (dog)	SLC12A3	XP_005617594.1
Bos taurus (cattle)	SLC12A3	NP_001193107.1
Danio rerio (zebrafish)	slc12a3	NP_001038545.1
Pan troglodytes (chimpanzee)	SLC12A3	XP_510983.2
Homo sapiens (human)	SLC12A3	NP_000330.2

Gene Ontology

The following SLC12A3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC12A3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu26218	NM_000339.2 Latest version!	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu108300	NM_001126107.2 Latest version!	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu108315	NM_000339.3 Latest version!	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu108407	NM_001126108.2 Latest version!	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu26429	NM_001126108.1 Latest version!	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$720.30 $1029.00
OHu26776	NM_001126107.1 Latest version!	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$727.30 $1039.00
OHu49480	XM_005256119.2 Latest version!	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC12A3 ( NM_000339.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000330.2 Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu26218
Clone ID Related Accession (Same CDS sequence)	NM_000339.2
Accession Version	NM_000339.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315298.1, U44128.1, AC012181.8 and BF510426.1. This sequence is a reference standard in the RefSeqGene project. On Apr 29, 2008 this sequence version replaced NM_000339.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U44128.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGCAGGAAGG CAGACACCTG 
CATGCCCTGG CCTTTGACAG CCGGCCCAGC CACGAGATGA CTGATGGGCT GGTGGAGGGC 
GAGGCAGGCA CCAGCAGCGA GAAGAACCCC GAGGAGCCAG TGCGCTTCGG CTGGGTCAAG 
GGGGTGATGA TTCGTTGCAT GCTCAACATT TGGGGCGTGA TCCTCTACCT GCGGCTGCCC 
TGGATTACGG CCCAGGCAGG CATCGTCCTG ACCTGGATCA TCATCCTGCT GTCGGTCACG 
GTGACCTCCA TCACAGGCCT CTCCATCTCA GCCATCTCCA CCAATGGCAA GGTCAAGTCA 
GGTGGCACCT ACTTCCTCAT CTCCCGGAGT CTGGGCCCAG AGCTTGGGGG CTCCATCGGC 
CTCATTTTCG CTTTCGCCAA TGCCGTGGGT GTGGCCATGC ACACGGTGGG CTTTGCAGAG 
ACCGTGCGGG ACCTGCTCCA GGAGTATGGG GCACCCATCG TGGACCCCAT TAACGACATC 
CGCATCATTG GCGTGGTCTC GGTCACTGTG CTGCTGGCCA TCTCCCTGGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTGTTCTTC CTTGTCATCA TGGTCTCCTT TGCCAACTAT 
TTAGTGGGGA CGCTGATCCC CCCATCTGAG GACAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGGGCGGACA TTTTTGTCCA GAACTTGGTG CCTGACTGGC GGGGTCCAGA TGGCACCTTC 
TTCGGAATGT TCTCCATCTT CTTCCCCTCG GCCACAGGCA TCCTGGCAGG GGCCAACATA 
TCTGGTGACC TCAAGGACCC TGCTATAGCC ATCCCCAAGG GGACCCTCAT GGCCATTTTC 
TGGACGACCA TTTCCTACCT GGCCATCTCA GCCACCATTG GCTCCTGCGT GGTGCGTGAT 
GCCTCTGGGG TCCTGAATGA CACAGTGACC CCTGGCTGGG GTGCCTGCGA GGGGCTGGCC 
TGCAGCTATG GCTGGAACTT CACCGAGTGC ACCCAGCAGC ACAGCTGCCA CTACGGCCTC 
ATCAACTATT ACCAGACCAT GAGCATGGTG TCAGGCTTCG CGCCCCTGAT CACGGCTGGC 
ATCTTCGGGG CCACCCTCTC CTCTGCCCTG GCCTGCCTTG TCTCTGCTGC CAAAGTCTTC 
CAGTGCCTTT GCGAGGACCA GCTGTACCCA CTGATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAAGG AGCCCGTGCG TGGCTACCTG CTGGCCTACG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATAGCCCCC ATCATTTCCA ACTTCTTCCT CTGCTCCTAT 
GCCCTCATCA ACTTCAGCTG CTTCCACGCC TCCATCACCA ACTCGCCTGG GTGGAGACCT 
TCATTCCAAT ACTACAACAA GTGGGCGGCG CTGTTTGGGG CTATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATTG GCGTGGTGCT CTTCCTCCTG 
CTCTATGTCA TCTACAAGAA GCCAGAGGTA AATTGGGGCT CCTCGGTACA GGCTGGCTCC 
TACAACCTGG CCCTCAGCTA CTCGGTGGGC CTCAATGAGG TGGAAGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTTGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCACGT GCTCATCGGA 
CCCCACAAGC AGAGGATGCC TGAGCTCCAG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAGGC CTTCTACTCG GATGTCATTG CCGAGGACCT CCGCAGAGGC 
GTCCAGATCC TCATGCAGGC CGCAGGTCTC GGGAGAATGA AGCCCAACAT TCTGGTGGTT 
GGGTTCAAGA AGAACTGGCA GTCGGCTCAC CCGGCCACAG TGGAAGACTA CATTGGCATC 
CTCCATGATG CCTTTGATTT CAACTATGGC GTGTGTGTCA TGAGGATGCG GGAGGGACTC 
AACGTGTCCA AGATGATGCA GGCGCACATT AACCCCGTGT TTGACCCAGC GGAGGACGGG 
AAGGAAGCCA GCGCCAGAGG TGCCAGGCCA TCAGTCTCTG GCGCTTTGGA CCCCAAGGCC 
CTGGTGAAGG AGGAGCAGGC CACCACCATC TTCCAGTCGG AGCAGGGCAA GAAGACCATA 
GACATCTACT GGCTCTTTGA CGATGGAGGC CTCACCCTCC TCATTCCCTA TCTCCTTGGC 
CGCAAGAGGA GGTGGAGCAA ATGCAAGATC CGTGTGTTCG TAGGCGGCCA GATTAACAGG 
ATGGACCAGG AGAGAAAGGC GATCATTTCT CTGCTGAGCA AGTTCCGACT GGGATTCCAT 
GAAGTCCACA TCCTCCCTGA CATCAACCAG AACCCTCGGG CTGAGCACAC CAAGAGGTTT 
GAGGACATGA TTGCACCCTT CCGTCTGAAT GATGGCTTCA AGGATGAGGC CACTGTCAAC 
GAGATGCGGC GGGACTGCCC CTGGAAGATC TCAGATGAGG AGATTACGAA GAACAGAGTC 
AAGTCCCTTC GGCAGGTGAG GCTGAATGAG ATTGTGCTGG ATTACTCCCG AGACGCTGCT 
CTCATCGTCA TCACTTTGCC CATAGGGAGG AAGGGGAAGT GCCCCAGCTC GCTGTACATG 
GCCTGGCTGG AGACCCTGTC CCAGGACCTC AGACCTCCAG TCATCCTGAT CCGAGGAAAC 
CAGGAAAACG TGCTCACCTT TTACTGCCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000330.2
CDS	30..3122
Translation	MAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSHPSHLTHSSTFCMRTFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHSFLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPINDIRIIGVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPAEDGKEASARGARPSVSGALDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	NM_000339.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000339.2

ORF Insert Sequence:

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGCAGGAAGG CAGACACCTG 
CATGCCCTGG CCTTTGACAG CCGGCCCAGC CACGAGATGA CTGATGGGCT GGTGGAGGGC 
GAGGCAGGCA CCAGCAGCGA GAAGAACCCC GAGGAGCCAG TGCGCTTCGG CTGGGTCAAG 
GGGGTGATGA TTCGTTGCAT GCTCAACATT TGGGGCGTGA TCCTCTACCT GCGGCTGCCC 
TGGATTACGG CCCAGGCAGG CATCGTCCTG ACCTGGATCA TCATCCTGCT GTCGGTCACG 
GTGACCTCCA TCACAGGCCT CTCCATCTCA GCCATCTCCA CCAATGGCAA GGTCAAGTCA 
GGTGGCACCT ACTTCCTCAT CTCCCGGAGT CTGGGCCCAG AGCTTGGGGG CTCCATCGGC 
CTCATTTTCG CTTTCGCCAA TGCCGTGGGT GTGGCCATGC ACACGGTGGG CTTTGCAGAG 
ACCGTGCGGG ACCTGCTCCA GGAGTATGGG GCACCCATCG TGGACCCCAT TAACGACATC 
CGCATCATTG GCGTGGTCTC GGTCACTGTG CTGCTGGCCA TCTCCCTGGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTGTTCTTC CTTGTCATCA TGGTCTCCTT TGCCAACTAT 
TTAGTGGGGA CGCTGATCCC CCCATCTGAG GACAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGGGCGGACA TTTTTGTCCA GAACTTGGTG CCTGACTGGC GGGGTCCAGA TGGCACCTTC 
TTCGGAATGT TCTCCATCTT CTTCCCCTCG GCCACAGGCA TCCTGGCAGG GGCCAACATA 
TCTGGTGACC TCAAGGACCC TGCTATAGCC ATCCCCAAGG GGACCCTCAT GGCCATTTTC 
TGGACGACCA TTTCCTACCT GGCCATCTCA GCCACCATTG GCTCCTGCGT GGTGCGTGAT 
GCCTCTGGGG TCCTGAATGA CACAGTGACC CCTGGCTGGG GTGCCTGCGA GGGGCTGGCC 
TGCAGCTATG GCTGGAACTT CACCGAGTGC ACCCAGCAGC ACAGCTGCCA CTACGGCCTC 
ATCAACTATT ACCAGACCAT GAGCATGGTG TCAGGCTTCG CGCCCCTGAT CACGGCTGGC 
ATCTTCGGGG CCACCCTCTC CTCTGCCCTG GCCTGCCTTG TCTCTGCTGC CAAAGTCTTC 
CAGTGCCTTT GCGAGGACCA GCTGTACCCA CTGATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAAGG AGCCCGTGCG TGGCTACCTG CTGGCCTACG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATAGCCCCC ATCATTTCCA ACTTCTTCCT CTGCTCCTAT 
GCCCTCATCA ACTTCAGCTG CTTCCACGCC TCCATCACCA ACTCGCCTGG GTGGAGACCT 
TCATTCCAAT ACTACAACAA GTGGGCGGCG CTGTTTGGGG CTATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATTG GCGTGGTGCT CTTCCTCCTG 
CTCTATGTCA TCTACAAGAA GCCAGAGGTA AATTGGGGCT CCTCGGTACA GGCTGGCTCC 
TACAACCTGG CCCTCAGCTA CTCGGTGGGC CTCAATGAGG TGGAAGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTTGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCACGT GCTCATCGGA 
CCCCACAAGC AGAGGATGCC TGAGCTCCAG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAGGC CTTCTACTCG GATGTCATTG CCGAGGACCT CCGCAGAGGC 
GTCCAGATCC TCATGCAGGC CGCAGGTCTC GGGAGAATGA AGCCCAACAT TCTGGTGGTT 
GGGTTCAAGA AGAACTGGCA GTCGGCTCAC CCGGCCACAG TGGAAGACTA CATTGGCATC 
CTCCATGATG CCTTTGATTT CAACTATGGC GTGTGTGTCA TGAGGATGCG GGAGGGACTC 
AACGTGTCCA AGATGATGCA GGCGCACATT AACCCCGTGT TTGACCCAGC GGAGGACGGG 
AAGGAAGCCA GCGCCAGAGG TGCCAGGCCA TCAGTCTCTG GCGCTTTGGA CCCCAAGGCC 
CTGGTGAAGG AGGAGCAGGC CACCACCATC TTCCAGTCGG AGCAGGGCAA GAAGACCATA 
GACATCTACT GGCTCTTTGA CGATGGAGGC CTCACCCTCC TCATTCCCTA TCTCCTTGGC 
CGCAAGAGGA GGTGGAGCAA ATGCAAGATC CGTGTGTTCG TAGGCGGCCA GATTAACAGG 
ATGGACCAGG AGAGAAAGGC GATCATTTCT CTGCTGAGCA AGTTCCGACT GGGATTCCAT 
GAAGTCCACA TCCTCCCTGA CATCAACCAG AACCCTCGGG CTGAGCACAC CAAGAGGTTT 
GAGGACATGA TTGCACCCTT CCGTCTGAAT GATGGCTTCA AGGATGAGGC CACTGTCAAC 
GAGATGCGGC GGGACTGCCC CTGGAAGATC TCAGATGAGG AGATTACGAA GAACAGAGTC 
AAGTCCCTTC GGCAGGTGAG GCTGAATGAG ATTGTGCTGG ATTACTCCCG AGACGCTGCT 
CTCATCGTCA TCACTTTGCC CATAGGGAGG AAGGGGAAGT GCCCCAGCTC GCTGTACATG 
GCCTGGCTGG AGACCCTGTC CCAGGACCTC AGACCTCCAG TCATCCTGAT CCGAGGAAAC 
CAGGAAAACG TGCTCACCTT TTACTGCCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A3 ( NM_001126107.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001119579.2 Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu108300
Clone ID Related Accession (Same CDS sequence)	NM_001126107.2
Accession Version	NM_001126107.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012181.8. On Jun 2, 2019 this sequence version replaced NM_001126107.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in a protein (isoform 2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315298.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGGAAGGCAG ACACCTGCAT 
GCCCTGGCCT TTGACAGCCG GCCCAGCCAC GAGATGACTG ATGGGCTGGT GGAGGGCGAG 
GCAGGCACCA GCAGCGAGAA GAACCCCGAG GAGCCAGTGC GCTTCGGCTG GGTCAAGGGG 
GTGATGATTC GTTGCATGCT CAACATTTGG GGCGTGATCC TCTACCTGCG GCTGCCCTGG 
ATTACGGCCC AGGCAGGCAT CGTCCTGACC TGGATCATCA TCCTGCTGTC GGTCACGGTG 
ACCTCCATCA CAGGCCTCTC CATCTCAGCC ATCTCCACCA ATGGCAAGGT CAAGTCAGGT 
GGCACCTACT TCCTCATCTC CCGGAGTCTG GGCCCAGAGC TTGGGGGCTC CATCGGCCTC 
ATTTTCGCTT TCGCCAATGC CGTGGGTGTG GCCATGCACA CGGTGGGCTT TGCAGAGACC 
GTGCGGGACC TGCTCCAGGA GTATGGGGCA CCCATCGTGG ACCCCATTAA CGACATCCGC 
ATCATTGCCG TGGTCTCGGT CACTGTGCTG CTGGCCATCT CCCTGGCTGG CATGGAGTGG 
GAGTCCAAGG CCCAGGTGCT GTTCTTCCTT GTCATCATGG TCTCCTTTGC CAACTATTTA 
GTGGGGACGC TGATCCCCCC ATCTGAGGAC AAGGCCTCCA AAGGCTTCTT CAGCTACCGG 
GCGGACATTT TTGTCCAGAA CTTGGTGCCT GACTGGCGGG GTCCAGATGG CACCTTCTTC 
GGAATGTTCT CCATCTTCTT CCCCTCGGCC ACAGGCATCC TGGCAGGGGC CAACATATCT 
GGTGACCTCA AGGACCCTGC TATAGCCATC CCCAAGGGGA CCCTCATGGC CATTTTCTGG 
ACGACCATTT CCTACCTGGC CATCTCAGCC ACCATTGGCT CCTGCGTGGT GCGTGATGCC 
TCTGGGGTCC TGAATGACAC AGTGACCCCT GGCTGGGGTG CCTGCGAGGG GCTGGCCTGC 
AGCTATGGCT GGAACTTCAC CGAGTGCACC CAGCAGCACA GCTGCCACTA CGGCCTCATC 
AACTATTACC AGACCATGAG CATGGTGTCA GGCTTCGCGC CCCTGATCAC GGCTGGCATC 
TTCGGGGCCA CCCTCTCCTC TGCCCTGGCC TGCCTTGTCT CTGCTGCCAA AGTCTTCCAG 
TGCCTTTGCG AGGACCAGCT GTACCCACTG ATCGGCTTCT TCGGCAAAGG CTATGGCAAG 
AACAAGGAGC CCGTGCGTGG CTACCTGCTG GCCTACGCCA TCGCTGTGGC CTTCATCATC 
ATCGCTGAGC TCAACACCAT AGCCCCCATC ATTTCCAACT TCTTCCTCTG CTCCTATGCC 
CTCATCAACT TCAGCTGCTT CCACGCCTCC ATCACCAACT CGCCTGGGTG GAGACCTTCA 
TTCCAATACT ACAACAAGTG GGCGGCGCTG TTTGGGGCTA TCATCTCCGT GGTCATCATG 
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATTGGCG TGGTGCTCTT CCTCCTGCTC 
TATGTCATCT ACAAGAAGCC AGAGGTAAAT TGGGGCTCCT CGGTACAGGC TGGCTCCTAC 
AACCTGGCCC TCAGCTACTC GGTGGGCCTC AATGAGGTGG AAGACCACAT CAAGAACTAC 
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTT 
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCACGTGCT CATCGGACCC 
CACAAGCAGA GGATGCCTGA GCTCCAGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC 
AAGAGGAAGA TCAAGGCCTT CTACTCGGAT GTCATTGCCG AGGACCTCCG CAGAGGCGTC 
CAGATCCTCA TGCAGGCCGC AGGTCTCGGG AGAATGAAGC CCAACATTCT GGTGGTTGGG 
TTCAAGAAGA ACTGGCAGTC GGCTCACCCG GCCACAGTGG AAGACTACAT TGGCATCCTC 
CATGATGCCT TTGATTTCAA CTATGGCGTG TGTGTCATGA GGATGCGGGA GGGACTCAAC 
GTGTCCAAGA TGATGCAGGC GCACATTAAC CCCGTGTTTG ACCCAGCGGA GGACGGGAAG 
GAAGCCAGCG CCAGAGGTGC CAGGCCATCA GTCTCTGGCG CTTTGGACCC CAAGGCCCTG 
GTGAAGGAGG AGCAGGCCAC CACCATCTTC CAGTCGGAGC AGGGCAAGAA GACCATAGAC 
ATCTACTGGC TCTTTGACGA TGGAGGCCTC ACCCTCCTCA TTCCCTATCT CCTTGGCCGC 
AAGAGGAGGT GGAGCAAATG CAAGATCCGT GTGTTCGTAG GCGGCCAGAT TAACAGGATG 
GACCAGGAGA GAAAGGCGAT CATTTCTCTG CTGAGCAAGT TCCGACTGGG ATTCCATGAA 
GTCCACATCC TCCCTGACAT CAACCAGAAC CCTCGGGCTG AGCACACCAA GAGGTTTGAG 
GACATGATTG CACCCTTCCG TCTGAATGAT GGCTTCAAGG ATGAGGCCAC TGTCAACGAG 
ATGCGGCGGG ACTGCCCCTG GAAGATCTCA GATGAGGAGA TTACGAAGAA CAGAGTCAAG 
TCCCTTCGGC AGGTGAGGCT GAATGAGATT GTGCTGGATT ACTCCCGAGA CGCTGCTCTC 
ATCGTCATCA CTTTGCCCAT AGGGAGGAAG GGGAAGTGCC CCAGCTCGCT GTACATGGCC 
TGGCTGGAGA CCCTGTCCCA GGACCTCAGA CCTCCAGTCA TCCTGATCCG AGGAAACCAG 
GAAAACGTGC TCACCTTTTA CTGCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119579.2
CDS	30..3119
Translation	MAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSHPSHLTHSSTFCMRTFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHSFLKEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPINDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPAEDGKEASARGARPSVSGALDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	NM_001126107.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126107.2

ORF Insert Sequence:

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGGAAGGCAG ACACCTGCAT 
GCCCTGGCCT TTGACAGCCG GCCCAGCCAC GAGATGACTG ATGGGCTGGT GGAGGGCGAG 
GCAGGCACCA GCAGCGAGAA GAACCCCGAG GAGCCAGTGC GCTTCGGCTG GGTCAAGGGG 
GTGATGATTC GTTGCATGCT CAACATTTGG GGCGTGATCC TCTACCTGCG GCTGCCCTGG 
ATTACGGCCC AGGCAGGCAT CGTCCTGACC TGGATCATCA TCCTGCTGTC GGTCACGGTG 
ACCTCCATCA CAGGCCTCTC CATCTCAGCC ATCTCCACCA ATGGCAAGGT CAAGTCAGGT 
GGCACCTACT TCCTCATCTC CCGGAGTCTG GGCCCAGAGC TTGGGGGCTC CATCGGCCTC 
ATTTTCGCTT TCGCCAATGC CGTGGGTGTG GCCATGCACA CGGTGGGCTT TGCAGAGACC 
GTGCGGGACC TGCTCCAGGA GTATGGGGCA CCCATCGTGG ACCCCATTAA CGACATCCGC 
ATCATTGCCG TGGTCTCGGT CACTGTGCTG CTGGCCATCT CCCTGGCTGG CATGGAGTGG 
GAGTCCAAGG CCCAGGTGCT GTTCTTCCTT GTCATCATGG TCTCCTTTGC CAACTATTTA 
GTGGGGACGC TGATCCCCCC ATCTGAGGAC AAGGCCTCCA AAGGCTTCTT CAGCTACCGG 
GCGGACATTT TTGTCCAGAA CTTGGTGCCT GACTGGCGGG GTCCAGATGG CACCTTCTTC 
GGAATGTTCT CCATCTTCTT CCCCTCGGCC ACAGGCATCC TGGCAGGGGC CAACATATCT 
GGTGACCTCA AGGACCCTGC TATAGCCATC CCCAAGGGGA CCCTCATGGC CATTTTCTGG 
ACGACCATTT CCTACCTGGC CATCTCAGCC ACCATTGGCT CCTGCGTGGT GCGTGATGCC 
TCTGGGGTCC TGAATGACAC AGTGACCCCT GGCTGGGGTG CCTGCGAGGG GCTGGCCTGC 
AGCTATGGCT GGAACTTCAC CGAGTGCACC CAGCAGCACA GCTGCCACTA CGGCCTCATC 
AACTATTACC AGACCATGAG CATGGTGTCA GGCTTCGCGC CCCTGATCAC GGCTGGCATC 
TTCGGGGCCA CCCTCTCCTC TGCCCTGGCC TGCCTTGTCT CTGCTGCCAA AGTCTTCCAG 
TGCCTTTGCG AGGACCAGCT GTACCCACTG ATCGGCTTCT TCGGCAAAGG CTATGGCAAG 
AACAAGGAGC CCGTGCGTGG CTACCTGCTG GCCTACGCCA TCGCTGTGGC CTTCATCATC 
ATCGCTGAGC TCAACACCAT AGCCCCCATC ATTTCCAACT TCTTCCTCTG CTCCTATGCC 
CTCATCAACT TCAGCTGCTT CCACGCCTCC ATCACCAACT CGCCTGGGTG GAGACCTTCA 
TTCCAATACT ACAACAAGTG GGCGGCGCTG TTTGGGGCTA TCATCTCCGT GGTCATCATG 
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATTGGCG TGGTGCTCTT CCTCCTGCTC 
TATGTCATCT ACAAGAAGCC AGAGGTAAAT TGGGGCTCCT CGGTACAGGC TGGCTCCTAC 
AACCTGGCCC TCAGCTACTC GGTGGGCCTC AATGAGGTGG AAGACCACAT CAAGAACTAC 
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTT 
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCACGTGCT CATCGGACCC 
CACAAGCAGA GGATGCCTGA GCTCCAGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC 
AAGAGGAAGA TCAAGGCCTT CTACTCGGAT GTCATTGCCG AGGACCTCCG CAGAGGCGTC 
CAGATCCTCA TGCAGGCCGC AGGTCTCGGG AGAATGAAGC CCAACATTCT GGTGGTTGGG 
TTCAAGAAGA ACTGGCAGTC GGCTCACCCG GCCACAGTGG AAGACTACAT TGGCATCCTC 
CATGATGCCT TTGATTTCAA CTATGGCGTG TGTGTCATGA GGATGCGGGA GGGACTCAAC 
GTGTCCAAGA TGATGCAGGC GCACATTAAC CCCGTGTTTG ACCCAGCGGA GGACGGGAAG 
GAAGCCAGCG CCAGAGGTGC CAGGCCATCA GTCTCTGGCG CTTTGGACCC CAAGGCCCTG 
GTGAAGGAGG AGCAGGCCAC CACCATCTTC CAGTCGGAGC AGGGCAAGAA GACCATAGAC 
ATCTACTGGC TCTTTGACGA TGGAGGCCTC ACCCTCCTCA TTCCCTATCT CCTTGGCCGC 
AAGAGGAGGT GGAGCAAATG CAAGATCCGT GTGTTCGTAG GCGGCCAGAT TAACAGGATG 
GACCAGGAGA GAAAGGCGAT CATTTCTCTG CTGAGCAAGT TCCGACTGGG ATTCCATGAA 
GTCCACATCC TCCCTGACAT CAACCAGAAC CCTCGGGCTG AGCACACCAA GAGGTTTGAG 
GACATGATTG CACCCTTCCG TCTGAATGAT GGCTTCAAGG ATGAGGCCAC TGTCAACGAG 
ATGCGGCGGG ACTGCCCCTG GAAGATCTCA GATGAGGAGA TTACGAAGAA CAGAGTCAAG 
TCCCTTCGGC AGGTGAGGCT GAATGAGATT GTGCTGGATT ACTCCCGAGA CGCTGCTCTC 
ATCGTCATCA CTTTGCCCAT AGGGAGGAAG GGGAAGTGCC CCAGCTCGCT GTACATGGCC 
TGGCTGGAGA CCCTGTCCCA GGACCTCAGA CCTCCAGTCA TCCTGATCCG AGGAAACCAG 
GAAAACGTGC TCACCTTTTA CTGCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A3 ( NM_000339.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000330.3 Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu108315
Clone ID Related Accession (Same CDS sequence)	NM_000339.3
Accession Version	NM_000339.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3093bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 3 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012181.8. On Jun 2, 2019 this sequence version replaced NM_000339.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: U44128.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGCAGGAAGG CAGACACCTG 
CATGCCCTGG CCTTTGACAG CCGGCCCAGC CACGAGATGA CTGATGGGCT GGTGGAGGGC 
GAGGCAGGCA CCAGCAGCGA GAAGAACCCC GAGGAGCCAG TGCGCTTCGG CTGGGTCAAG 
GGGGTGATGA TTCGTTGCAT GCTCAACATT TGGGGCGTGA TCCTCTACCT GCGGCTGCCC 
TGGATTACGG CCCAGGCAGG CATCGTCCTG ACCTGGATCA TCATCCTGCT GTCGGTCACG 
GTGACCTCCA TCACAGGCCT CTCCATCTCA GCCATCTCCA CCAATGGCAA GGTCAAGTCA 
GGTGGCACCT ACTTCCTCAT CTCCCGGAGT CTGGGCCCAG AGCTTGGGGG CTCCATCGGC 
CTCATTTTCG CTTTCGCCAA TGCCGTGGGT GTGGCCATGC ACACGGTGGG CTTTGCAGAG 
ACCGTGCGGG ACCTGCTCCA GGAGTATGGG GCACCCATCG TGGACCCCAT TAACGACATC 
CGCATCATTG CCGTGGTCTC GGTCACTGTG CTGCTGGCCA TCTCCCTGGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTGTTCTTC CTTGTCATCA TGGTCTCCTT TGCCAACTAT 
TTAGTGGGGA CGCTGATCCC CCCATCTGAG GACAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGGGCGGACA TTTTTGTCCA GAACTTGGTG CCTGACTGGC GGGGTCCAGA TGGCACCTTC 
TTCGGAATGT TCTCCATCTT CTTCCCCTCG GCCACAGGCA TCCTGGCAGG GGCCAACATA 
TCTGGTGACC TCAAGGACCC TGCTATAGCC ATCCCCAAGG GGACCCTCAT GGCCATTTTC 
TGGACGACCA TTTCCTACCT GGCCATCTCA GCCACCATTG GCTCCTGCGT GGTGCGTGAT 
GCCTCTGGGG TCCTGAATGA CACAGTGACC CCTGGCTGGG GTGCCTGCGA GGGGCTGGCC 
TGCAGCTATG GCTGGAACTT CACCGAGTGC ACCCAGCAGC ACAGCTGCCA CTACGGCCTC 
ATCAACTATT ACCAGACCAT GAGCATGGTG TCAGGCTTCG CGCCCCTGAT CACGGCTGGC 
ATCTTCGGGG CCACCCTCTC CTCTGCCCTG GCCTGCCTTG TCTCTGCTGC CAAAGTCTTC 
CAGTGCCTTT GCGAGGACCA GCTGTACCCA CTGATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAAGG AGCCCGTGCG TGGCTACCTG CTGGCCTACG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATAGCCCCC ATCATTTCCA ACTTCTTCCT CTGCTCCTAT 
GCCCTCATCA ACTTCAGCTG CTTCCACGCC TCCATCACCA ACTCGCCTGG GTGGAGACCT 
TCATTCCAAT ACTACAACAA GTGGGCGGCG CTGTTTGGGG CTATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATTG GCGTGGTGCT CTTCCTCCTG 
CTCTATGTCA TCTACAAGAA GCCAGAGGTA AATTGGGGCT CCTCGGTACA GGCTGGCTCC 
TACAACCTGG CCCTCAGCTA CTCGGTGGGC CTCAATGAGG TGGAAGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTTGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCACGT GCTCATCGGA 
CCCCACAAGC AGAGGATGCC TGAGCTCCAG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAGGC CTTCTACTCG GATGTCATTG CCGAGGACCT CCGCAGAGGC 
GTCCAGATCC TCATGCAGGC CGCAGGTCTC GGGAGAATGA AGCCCAACAT TCTGGTGGTT 
GGGTTCAAGA AGAACTGGCA GTCGGCTCAC CCGGCCACAG TGGAAGACTA CATTGGCATC 
CTCCATGATG CCTTTGATTT CAACTATGGC GTGTGTGTCA TGAGGATGCG GGAGGGACTC 
AACGTGTCCA AGATGATGCA GGCGCACATT AACCCCGTGT TTGACCCAGC GGAGGACGGG 
AAGGAAGCCA GCGCCAGAGG TGCCAGGCCA TCAGTCTCTG GCGCTTTGGA CCCCAAGGCC 
CTGGTGAAGG AGGAGCAGGC CACCACCATC TTCCAGTCGG AGCAGGGCAA GAAGACCATA 
GACATCTACT GGCTCTTTGA CGATGGAGGC CTCACCCTCC TCATTCCCTA TCTCCTTGGC 
CGCAAGAGGA GGTGGAGCAA ATGCAAGATC CGTGTGTTCG TAGGCGGCCA GATTAACAGG 
ATGGACCAGG AGAGAAAGGC GATCATTTCT CTGCTGAGCA AGTTCCGACT GGGATTCCAT 
GAAGTCCACA TCCTCCCTGA CATCAACCAG AACCCTCGGG CTGAGCACAC CAAGAGGTTT 
GAGGACATGA TTGCACCCTT CCGTCTGAAT GATGGCTTCA AGGATGAGGC CACTGTCAAC 
GAGATGCGGC GGGACTGCCC CTGGAAGATC TCAGATGAGG AGATTACGAA GAACAGAGTC 
AAGTCCCTTC GGCAGGTGAG GCTGAATGAG ATTGTGCTGG ATTACTCCCG AGACGCTGCT 
CTCATCGTCA TCACTTTGCC CATAGGGAGG AAGGGGAAGT GCCCCAGCTC GCTGTACATG 
GCCTGGCTGG AGACCCTGTC CCAGGACCTC AGACCTCCAG TCATCCTGAT CCGAGGAAAC 
CAGGAAAACG TGCTCACCTT TTACTGCCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000330.3
CDS	30..3122
Translation	MAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSHPSHLTHSSTFCMRTFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHSFLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPINDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPAEDGKEASARGARPSVSGALDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	NM_000339.3
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000339.3

ORF Insert Sequence:

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGCAGGAAGG CAGACACCTG 
CATGCCCTGG CCTTTGACAG CCGGCCCAGC CACGAGATGA CTGATGGGCT GGTGGAGGGC 
GAGGCAGGCA CCAGCAGCGA GAAGAACCCC GAGGAGCCAG TGCGCTTCGG CTGGGTCAAG 
GGGGTGATGA TTCGTTGCAT GCTCAACATT TGGGGCGTGA TCCTCTACCT GCGGCTGCCC 
TGGATTACGG CCCAGGCAGG CATCGTCCTG ACCTGGATCA TCATCCTGCT GTCGGTCACG 
GTGACCTCCA TCACAGGCCT CTCCATCTCA GCCATCTCCA CCAATGGCAA GGTCAAGTCA 
GGTGGCACCT ACTTCCTCAT CTCCCGGAGT CTGGGCCCAG AGCTTGGGGG CTCCATCGGC 
CTCATTTTCG CTTTCGCCAA TGCCGTGGGT GTGGCCATGC ACACGGTGGG CTTTGCAGAG 
ACCGTGCGGG ACCTGCTCCA GGAGTATGGG GCACCCATCG TGGACCCCAT TAACGACATC 
CGCATCATTG CCGTGGTCTC GGTCACTGTG CTGCTGGCCA TCTCCCTGGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTGTTCTTC CTTGTCATCA TGGTCTCCTT TGCCAACTAT 
TTAGTGGGGA CGCTGATCCC CCCATCTGAG GACAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGGGCGGACA TTTTTGTCCA GAACTTGGTG CCTGACTGGC GGGGTCCAGA TGGCACCTTC 
TTCGGAATGT TCTCCATCTT CTTCCCCTCG GCCACAGGCA TCCTGGCAGG GGCCAACATA 
TCTGGTGACC TCAAGGACCC TGCTATAGCC ATCCCCAAGG GGACCCTCAT GGCCATTTTC 
TGGACGACCA TTTCCTACCT GGCCATCTCA GCCACCATTG GCTCCTGCGT GGTGCGTGAT 
GCCTCTGGGG TCCTGAATGA CACAGTGACC CCTGGCTGGG GTGCCTGCGA GGGGCTGGCC 
TGCAGCTATG GCTGGAACTT CACCGAGTGC ACCCAGCAGC ACAGCTGCCA CTACGGCCTC 
ATCAACTATT ACCAGACCAT GAGCATGGTG TCAGGCTTCG CGCCCCTGAT CACGGCTGGC 
ATCTTCGGGG CCACCCTCTC CTCTGCCCTG GCCTGCCTTG TCTCTGCTGC CAAAGTCTTC 
CAGTGCCTTT GCGAGGACCA GCTGTACCCA CTGATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAAGG AGCCCGTGCG TGGCTACCTG CTGGCCTACG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATAGCCCCC ATCATTTCCA ACTTCTTCCT CTGCTCCTAT 
GCCCTCATCA ACTTCAGCTG CTTCCACGCC TCCATCACCA ACTCGCCTGG GTGGAGACCT 
TCATTCCAAT ACTACAACAA GTGGGCGGCG CTGTTTGGGG CTATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATTG GCGTGGTGCT CTTCCTCCTG 
CTCTATGTCA TCTACAAGAA GCCAGAGGTA AATTGGGGCT CCTCGGTACA GGCTGGCTCC 
TACAACCTGG CCCTCAGCTA CTCGGTGGGC CTCAATGAGG TGGAAGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTTGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCACGT GCTCATCGGA 
CCCCACAAGC AGAGGATGCC TGAGCTCCAG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAGGC CTTCTACTCG GATGTCATTG CCGAGGACCT CCGCAGAGGC 
GTCCAGATCC TCATGCAGGC CGCAGGTCTC GGGAGAATGA AGCCCAACAT TCTGGTGGTT 
GGGTTCAAGA AGAACTGGCA GTCGGCTCAC CCGGCCACAG TGGAAGACTA CATTGGCATC 
CTCCATGATG CCTTTGATTT CAACTATGGC GTGTGTGTCA TGAGGATGCG GGAGGGACTC 
AACGTGTCCA AGATGATGCA GGCGCACATT AACCCCGTGT TTGACCCAGC GGAGGACGGG 
AAGGAAGCCA GCGCCAGAGG TGCCAGGCCA TCAGTCTCTG GCGCTTTGGA CCCCAAGGCC 
CTGGTGAAGG AGGAGCAGGC CACCACCATC TTCCAGTCGG AGCAGGGCAA GAAGACCATA 
GACATCTACT GGCTCTTTGA CGATGGAGGC CTCACCCTCC TCATTCCCTA TCTCCTTGGC 
CGCAAGAGGA GGTGGAGCAA ATGCAAGATC CGTGTGTTCG TAGGCGGCCA GATTAACAGG 
ATGGACCAGG AGAGAAAGGC GATCATTTCT CTGCTGAGCA AGTTCCGACT GGGATTCCAT 
GAAGTCCACA TCCTCCCTGA CATCAACCAG AACCCTCGGG CTGAGCACAC CAAGAGGTTT 
GAGGACATGA TTGCACCCTT CCGTCTGAAT GATGGCTTCA AGGATGAGGC CACTGTCAAC 
GAGATGCGGC GGGACTGCCC CTGGAAGATC TCAGATGAGG AGATTACGAA GAACAGAGTC 
AAGTCCCTTC GGCAGGTGAG GCTGAATGAG ATTGTGCTGG ATTACTCCCG AGACGCTGCT 
CTCATCGTCA TCACTTTGCC CATAGGGAGG AAGGGGAAGT GCCCCAGCTC GCTGTACATG 
GCCTGGCTGG AGACCCTGTC CCAGGACCTC AGACCTCCAG TCATCCTGAT CCGAGGAAAC 
CAGGAAAACG TGCTCACCTT TTACTGCCAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A3 ( NM_001126108.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001119580.2 Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu108407
Clone ID Related Accession (Same CDS sequence)	NM_001126108.2
Accession Version	NM_001126108.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 3 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012181.8. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_001126108.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a protein (isoform 3) that is 9 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X91220.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGCAGGAAGG CAGACACCTG 
CATGCCCTGG CCTTTGACAG CCGGCCCAGC CACGAGATGA CTGATGGGCT GGTGGAGGGC 
GAGGCAGGCA CCAGCAGCGA GAAGAACCCC GAGGAGCCAG TGCGCTTCGG CTGGGTCAAG 
GGGGTGATGA TTCGTTGCAT GCTCAACATT TGGGGCGTGA TCCTCTACCT GCGGCTGCCC 
TGGATTACGG CCCAGGCAGG CATCGTCCTG ACCTGGATCA TCATCCTGCT GTCGGTCACG 
GTGACCTCCA TCACAGGCCT CTCCATCTCA GCCATCTCCA CCAATGGCAA GGTCAAGTCA 
GGTGGCACCT ACTTCCTCAT CTCCCGGAGT CTGGGCCCAG AGCTTGGGGG CTCCATCGGC 
CTCATTTTCG CTTTCGCCAA TGCCGTGGGT GTGGCCATGC ACACGGTGGG CTTTGCAGAG 
ACCGTGCGGG ACCTGCTCCA GGAGTATGGG GCACCCATCG TGGACCCCAT TAACGACATC 
CGCATCATTG CCGTGGTCTC GGTCACTGTG CTGCTGGCCA TCTCCCTGGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTGTTCTTC CTTGTCATCA TGGTCTCCTT TGCCAACTAT 
TTAGTGGGGA CGCTGATCCC CCCATCTGAG GACAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGGGCGGACA TTTTTGTCCA GAACTTGGTG CCTGACTGGC GGGGTCCAGA TGGCACCTTC 
TTCGGAATGT TCTCCATCTT CTTCCCCTCG GCCACAGGCA TCCTGGCAGG GGCCAACATA 
TCTGGTGACC TCAAGGACCC TGCTATAGCC ATCCCCAAGG GGACCCTCAT GGCCATTTTC 
TGGACGACCA TTTCCTACCT GGCCATCTCA GCCACCATTG GCTCCTGCGT GGTGCGTGAT 
GCCTCTGGGG TCCTGAATGA CACAGTGACC CCTGGCTGGG GTGCCTGCGA GGGGCTGGCC 
TGCAGCTATG GCTGGAACTT CACCGAGTGC ACCCAGCAGC ACAGCTGCCA CTACGGCCTC 
ATCAACTATT ACCAGACCAT GAGCATGGTG TCAGGCTTCG CGCCCCTGAT CACGGCTGGC 
ATCTTCGGGG CCACCCTCTC CTCTGCCCTG GCCTGCCTTG TCTCTGCTGC CAAAGTCTTC 
CAGTGCCTTT GCGAGGACCA GCTGTACCCA CTGATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAAGG AGCCCGTGCG TGGCTACCTG CTGGCCTACG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATAGCCCCC ATCATTTCCA ACTTCTTCCT CTGCTCCTAT 
GCCCTCATCA ACTTCAGCTG CTTCCACGCC TCCATCACCA ACTCGCCTGG GTGGAGACCT 
TCATTCCAAT ACTACAACAA GTGGGCGGCG CTGTTTGGGG CTATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATTG GCGTGGTGCT CTTCCTCCTG 
CTCTATGTCA TCTACAAGAA GCCAGAGGTA AATTGGGGCT CCTCGGTACA GGCTGGCTCC 
TACAACCTGG CCCTCAGCTA CTCGGTGGGC CTCAATGAGG TGGAAGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTTGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCACGT GCTCATCGGA 
CCCCACAAGC AGAGGATGCC TGAGCTCCAG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAGGC CTTCTACTCG GATGTCATTG CCGAGGACCT CCGCAGAGGC 
GTCCAGATCC TCATGCAGGC CGCAGGTCTC GGGAGAATGA AGCCCAACAT TCTGGTGGTT 
GGGTTCAAGA AGAACTGGCA GTCGGCTCAC CCGGCCACAG TGGAAGACTA CATTGGCATC 
CTCCATGATG CCTTTGATTT CAACTATGGC GTGTGTGTCA TGAGGATGCG GGAGGGACTC 
AACGTGTCCA AGATGATGCA GGCGCACATT AACCCCGTGT TTGACCCAGC GGAGGACGGG 
AAGGAAGCCA GCGCCAGAGT GGACCCCAAG GCCCTGGTGA AGGAGGAGCA GGCCACCACC 
ATCTTCCAGT CGGAGCAGGG CAAGAAGACC ATAGACATCT ACTGGCTCTT TGACGATGGA 
GGCCTCACCC TCCTCATTCC CTATCTCCTT GGCCGCAAGA GGAGGTGGAG CAAATGCAAG 
ATCCGTGTGT TCGTAGGCGG CCAGATTAAC AGGATGGACC AGGAGAGAAA GGCGATCATT 
TCTCTGCTGA GCAAGTTCCG ACTGGGATTC CATGAAGTCC ACATCCTCCC TGACATCAAC 
CAGAACCCTC GGGCTGAGCA CACCAAGAGG TTTGAGGACA TGATTGCACC CTTCCGTCTG 
AATGATGGCT TCAAGGATGA GGCCACTGTC AACGAGATGC GGCGGGACTG CCCCTGGAAG 
ATCTCAGATG AGGAGATTAC GAAGAACAGA GTCAAGTCCC TTCGGCAGGT GAGGCTGAAT 
GAGATTGTGC TGGATTACTC CCGAGACGCT GCTCTCATCG TCATCACTTT GCCCATAGGG 
AGGAAGGGGA AGTGCCCCAG CTCGCTGTAC ATGGCCTGGC TGGAGACCCT GTCCCAGGAC 
CTCAGACCTC CAGTCATCCT GATCCGAGGA AACCAGGAAA ACGTGCTCAC CTTTTACTGC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119580.2
CDS	30..3095
Translation	MAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSHPSHLTHSSTFCMRTFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHSFLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPINDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPAEDGKEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	NM_001126108.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126108.2

ORF Insert Sequence:

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGCAGGAAGG CAGACACCTG 
CATGCCCTGG CCTTTGACAG CCGGCCCAGC CACGAGATGA CTGATGGGCT GGTGGAGGGC 
GAGGCAGGCA CCAGCAGCGA GAAGAACCCC GAGGAGCCAG TGCGCTTCGG CTGGGTCAAG 
GGGGTGATGA TTCGTTGCAT GCTCAACATT TGGGGCGTGA TCCTCTACCT GCGGCTGCCC 
TGGATTACGG CCCAGGCAGG CATCGTCCTG ACCTGGATCA TCATCCTGCT GTCGGTCACG 
GTGACCTCCA TCACAGGCCT CTCCATCTCA GCCATCTCCA CCAATGGCAA GGTCAAGTCA 
GGTGGCACCT ACTTCCTCAT CTCCCGGAGT CTGGGCCCAG AGCTTGGGGG CTCCATCGGC 
CTCATTTTCG CTTTCGCCAA TGCCGTGGGT GTGGCCATGC ACACGGTGGG CTTTGCAGAG 
ACCGTGCGGG ACCTGCTCCA GGAGTATGGG GCACCCATCG TGGACCCCAT TAACGACATC 
CGCATCATTG CCGTGGTCTC GGTCACTGTG CTGCTGGCCA TCTCCCTGGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTGTTCTTC CTTGTCATCA TGGTCTCCTT TGCCAACTAT 
TTAGTGGGGA CGCTGATCCC CCCATCTGAG GACAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGGGCGGACA TTTTTGTCCA GAACTTGGTG CCTGACTGGC GGGGTCCAGA TGGCACCTTC 
TTCGGAATGT TCTCCATCTT CTTCCCCTCG GCCACAGGCA TCCTGGCAGG GGCCAACATA 
TCTGGTGACC TCAAGGACCC TGCTATAGCC ATCCCCAAGG GGACCCTCAT GGCCATTTTC 
TGGACGACCA TTTCCTACCT GGCCATCTCA GCCACCATTG GCTCCTGCGT GGTGCGTGAT 
GCCTCTGGGG TCCTGAATGA CACAGTGACC CCTGGCTGGG GTGCCTGCGA GGGGCTGGCC 
TGCAGCTATG GCTGGAACTT CACCGAGTGC ACCCAGCAGC ACAGCTGCCA CTACGGCCTC 
ATCAACTATT ACCAGACCAT GAGCATGGTG TCAGGCTTCG CGCCCCTGAT CACGGCTGGC 
ATCTTCGGGG CCACCCTCTC CTCTGCCCTG GCCTGCCTTG TCTCTGCTGC CAAAGTCTTC 
CAGTGCCTTT GCGAGGACCA GCTGTACCCA CTGATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAAGG AGCCCGTGCG TGGCTACCTG CTGGCCTACG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATAGCCCCC ATCATTTCCA ACTTCTTCCT CTGCTCCTAT 
GCCCTCATCA ACTTCAGCTG CTTCCACGCC TCCATCACCA ACTCGCCTGG GTGGAGACCT 
TCATTCCAAT ACTACAACAA GTGGGCGGCG CTGTTTGGGG CTATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATTG GCGTGGTGCT CTTCCTCCTG 
CTCTATGTCA TCTACAAGAA GCCAGAGGTA AATTGGGGCT CCTCGGTACA GGCTGGCTCC 
TACAACCTGG CCCTCAGCTA CTCGGTGGGC CTCAATGAGG TGGAAGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTTGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCACGT GCTCATCGGA 
CCCCACAAGC AGAGGATGCC TGAGCTCCAG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAGGC CTTCTACTCG GATGTCATTG CCGAGGACCT CCGCAGAGGC 
GTCCAGATCC TCATGCAGGC CGCAGGTCTC GGGAGAATGA AGCCCAACAT TCTGGTGGTT 
GGGTTCAAGA AGAACTGGCA GTCGGCTCAC CCGGCCACAG TGGAAGACTA CATTGGCATC 
CTCCATGATG CCTTTGATTT CAACTATGGC GTGTGTGTCA TGAGGATGCG GGAGGGACTC 
AACGTGTCCA AGATGATGCA GGCGCACATT AACCCCGTGT TTGACCCAGC GGAGGACGGG 
AAGGAAGCCA GCGCCAGAGT GGACCCCAAG GCCCTGGTGA AGGAGGAGCA GGCCACCACC 
ATCTTCCAGT CGGAGCAGGG CAAGAAGACC ATAGACATCT ACTGGCTCTT TGACGATGGA 
GGCCTCACCC TCCTCATTCC CTATCTCCTT GGCCGCAAGA GGAGGTGGAG CAAATGCAAG 
ATCCGTGTGT TCGTAGGCGG CCAGATTAAC AGGATGGACC AGGAGAGAAA GGCGATCATT 
TCTCTGCTGA GCAAGTTCCG ACTGGGATTC CATGAAGTCC ACATCCTCCC TGACATCAAC 
CAGAACCCTC GGGCTGAGCA CACCAAGAGG TTTGAGGACA TGATTGCACC CTTCCGTCTG 
AATGATGGCT TCAAGGATGA GGCCACTGTC AACGAGATGC GGCGGGACTG CCCCTGGAAG 
ATCTCAGATG AGGAGATTAC GAAGAACAGA GTCAAGTCCC TTCGGCAGGT GAGGCTGAAT 
GAGATTGTGC TGGATTACTC CCGAGACGCT GCTCTCATCG TCATCACTTT GCCCATAGGG 
AGGAAGGGGA AGTGCCCCAG CTCGCTGTAC ATGGCCTGGC TGGAGACCCT GTCCCAGGAC 
CTCAGACCTC CAGTCATCCT GATCCGAGGA AACCAGGAAA ACGTGCTCAC CTTTTACTGC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A3 ( NM_001126108.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001119580.1 Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 3, mRNA.

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CloneID	OHu26429
Clone ID Related Accession (Same CDS sequence)	NM_001126108.1
Accession Version	NM_001126108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3066bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 3 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315298.1, X91220.1, AC012181.8 and BF510426.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 3' coding region, compared to variant 1, resulting in a protein (isoform 3) that is 9 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X91220.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGCAGGAAGG CAGACACCTG 
CATGCCCTGG CCTTTGACAG CCGGCCCAGC CACGAGATGA CTGATGGGCT GGTGGAGGGC 
GAGGCAGGCA CCAGCAGCGA GAAGAACCCC GAGGAGCCAG TGCGCTTCGG CTGGGTCAAG 
GGGGTGATGA TTCGTTGCAT GCTCAACATT TGGGGCGTGA TCCTCTACCT GCGGCTGCCC 
TGGATTACGG CCCAGGCAGG CATCGTCCTG ACCTGGATCA TCATCCTGCT GTCGGTCACG 
GTGACCTCCA TCACAGGCCT CTCCATCTCA GCCATCTCCA CCAATGGCAA GGTCAAGTCA 
GGTGGCACCT ACTTCCTCAT CTCCCGGAGT CTGGGCCCAG AGCTTGGGGG CTCCATCGGC 
CTCATTTTCG CTTTCGCCAA TGCCGTGGGT GTGGCCATGC ACACGGTGGG CTTTGCAGAG 
ACCGTGCGGG ACCTGCTCCA GGAGTATGGG GCACCCATCG TGGACCCCAT TAACGACATC 
CGCATCATTG GCGTGGTCTC GGTCACTGTG CTGCTGGCCA TCTCCCTGGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTGTTCTTC CTTGTCATCA TGGTCTCCTT TGCCAACTAT 
TTAGTGGGGA CGCTGATCCC CCCATCTGAG GACAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGGGCGGACA TTTTTGTCCA GAACTTGGTG CCTGACTGGC GGGGTCCAGA TGGCACCTTC 
TTCGGAATGT TCTCCATCTT CTTCCCCTCG GCCACAGGCA TCCTGGCAGG GGCCAACATA 
TCTGGTGACC TCAAGGACCC TGCTATAGCC ATCCCCAAGG GGACCCTCAT GGCCATTTTC 
TGGACGACCA TTTCCTACCT GGCCATCTCA GCCACCATTG GCTCCTGCGT GGTGCGTGAT 
GCCTCTGGGG TCCTGAATGA CACAGTGACC CCTGGCTGGG GTGCCTGCGA GGGGCTGGCC 
TGCAGCTATG GCTGGAACTT CACCGAGTGC ACCCAGCAGC ACAGCTGCCA CTACGGCCTC 
ATCAACTATT ACCAGACCAT GAGCATGGTG TCAGGCTTCG CGCCCCTGAT CACGGCTGGC 
ATCTTCGGGG CCACCCTCTC CTCTGCCCTG GCCTGCCTTG TCTCTGCTGC CAAAGTCTTC 
CAGTGCCTTT GCGAGGACCA GCTGTACCCA CTGATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAAGG AGCCCGTGCG TGGCTACCTG CTGGCCTACG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATAGCCCCC ATCATTTCCA ACTTCTTCCT CTGCTCCTAT 
GCCCTCATCA ACTTCAGCTG CTTCCACGCC TCCATCACCA ACTCGCCTGG GTGGAGACCT 
TCATTCCAAT ACTACAACAA GTGGGCGGCG CTGTTTGGGG CTATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATTG GCGTGGTGCT CTTCCTCCTG 
CTCTATGTCA TCTACAAGAA GCCAGAGGTA AATTGGGGCT CCTCGGTACA GGCTGGCTCC 
TACAACCTGG CCCTCAGCTA CTCGGTGGGC CTCAATGAGG TGGAAGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTTGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCACGT GCTCATCGGA 
CCCCACAAGC AGAGGATGCC TGAGCTCCAG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAGGC CTTCTACTCG GATGTCATTG CCGAGGACCT CCGCAGAGGC 
GTCCAGATCC TCATGCAGGC CGCAGGTCTC GGGAGAATGA AGCCCAACAT TCTGGTGGTT 
GGGTTCAAGA AGAACTGGCA GTCGGCTCAC CCGGCCACAG TGGAAGACTA CATTGGCATC 
CTCCATGATG CCTTTGATTT CAACTATGGC GTGTGTGTCA TGAGGATGCG GGAGGGACTC 
AACGTGTCCA AGATGATGCA GGCGCACATT AACCCCGTGT TTGACCCAGC GGAGGACGGG 
AAGGAAGCCA GCGCCAGAGT GGACCCCAAG GCCCTGGTGA AGGAGGAGCA GGCCACCACC 
ATCTTCCAGT CGGAGCAGGG CAAGAAGACC ATAGACATCT ACTGGCTCTT TGACGATGGA 
GGCCTCACCC TCCTCATTCC CTATCTCCTT GGCCGCAAGA GGAGGTGGAG CAAATGCAAG 
ATCCGTGTGT TCGTAGGCGG CCAGATTAAC AGGATGGACC AGGAGAGAAA GGCGATCATT 
TCTCTGCTGA GCAAGTTCCG ACTGGGATTC CATGAAGTCC ACATCCTCCC TGACATCAAC 
CAGAACCCTC GGGCTGAGCA CACCAAGAGG TTTGAGGACA TGATTGCACC CTTCCGTCTG 
AATGATGGCT TCAAGGATGA GGCCACTGTC AACGAGATGC GGCGGGACTG CCCCTGGAAG 
ATCTCAGATG AGGAGATTAC GAAGAACAGA GTCAAGTCCC TTCGGCAGGT GAGGCTGAAT 
GAGATTGTGC TGGATTACTC CCGAGACGCT GCTCTCATCG TCATCACTTT GCCCATAGGG 
AGGAAGGGGA AGTGCCCCAG CTCGCTGTAC ATGGCCTGGC TGGAGACCCT GTCCCAGGAC 
CTCAGACCTC CAGTCATCCT GATCCGAGGA AACCAGGAAA ACGTGCTCAC CTTTTACTGC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119580.1
CDS	30..3095
Translation	MAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSHPSHLTHSSTFCMRTFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHSFLKQEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPINDIRIIGVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPAEDGKEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	NM_001126108.1
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126108.1

ORF Insert Sequence:

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGCAGGAAGG CAGACACCTG 
CATGCCCTGG CCTTTGACAG CCGGCCCAGC CACGAGATGA CTGATGGGCT GGTGGAGGGC 
GAGGCAGGCA CCAGCAGCGA GAAGAACCCC GAGGAGCCAG TGCGCTTCGG CTGGGTCAAG 
GGGGTGATGA TTCGTTGCAT GCTCAACATT TGGGGCGTGA TCCTCTACCT GCGGCTGCCC 
TGGATTACGG CCCAGGCAGG CATCGTCCTG ACCTGGATCA TCATCCTGCT GTCGGTCACG 
GTGACCTCCA TCACAGGCCT CTCCATCTCA GCCATCTCCA CCAATGGCAA GGTCAAGTCA 
GGTGGCACCT ACTTCCTCAT CTCCCGGAGT CTGGGCCCAG AGCTTGGGGG CTCCATCGGC 
CTCATTTTCG CTTTCGCCAA TGCCGTGGGT GTGGCCATGC ACACGGTGGG CTTTGCAGAG 
ACCGTGCGGG ACCTGCTCCA GGAGTATGGG GCACCCATCG TGGACCCCAT TAACGACATC 
CGCATCATTG GCGTGGTCTC GGTCACTGTG CTGCTGGCCA TCTCCCTGGC TGGCATGGAG 
TGGGAGTCCA AGGCCCAGGT GCTGTTCTTC CTTGTCATCA TGGTCTCCTT TGCCAACTAT 
TTAGTGGGGA CGCTGATCCC CCCATCTGAG GACAAGGCCT CCAAAGGCTT CTTCAGCTAC 
CGGGCGGACA TTTTTGTCCA GAACTTGGTG CCTGACTGGC GGGGTCCAGA TGGCACCTTC 
TTCGGAATGT TCTCCATCTT CTTCCCCTCG GCCACAGGCA TCCTGGCAGG GGCCAACATA 
TCTGGTGACC TCAAGGACCC TGCTATAGCC ATCCCCAAGG GGACCCTCAT GGCCATTTTC 
TGGACGACCA TTTCCTACCT GGCCATCTCA GCCACCATTG GCTCCTGCGT GGTGCGTGAT 
GCCTCTGGGG TCCTGAATGA CACAGTGACC CCTGGCTGGG GTGCCTGCGA GGGGCTGGCC 
TGCAGCTATG GCTGGAACTT CACCGAGTGC ACCCAGCAGC ACAGCTGCCA CTACGGCCTC 
ATCAACTATT ACCAGACCAT GAGCATGGTG TCAGGCTTCG CGCCCCTGAT CACGGCTGGC 
ATCTTCGGGG CCACCCTCTC CTCTGCCCTG GCCTGCCTTG TCTCTGCTGC CAAAGTCTTC 
CAGTGCCTTT GCGAGGACCA GCTGTACCCA CTGATCGGCT TCTTCGGCAA AGGCTATGGC 
AAGAACAAGG AGCCCGTGCG TGGCTACCTG CTGGCCTACG CCATCGCTGT GGCCTTCATC 
ATCATCGCTG AGCTCAACAC CATAGCCCCC ATCATTTCCA ACTTCTTCCT CTGCTCCTAT 
GCCCTCATCA ACTTCAGCTG CTTCCACGCC TCCATCACCA ACTCGCCTGG GTGGAGACCT 
TCATTCCAAT ACTACAACAA GTGGGCGGCG CTGTTTGGGG CTATCATCTC CGTGGTCATC 
ATGTTCCTCC TCACCTGGTG GGCGGCCCTC ATCGCCATTG GCGTGGTGCT CTTCCTCCTG 
CTCTATGTCA TCTACAAGAA GCCAGAGGTA AATTGGGGCT CCTCGGTACA GGCTGGCTCC 
TACAACCTGG CCCTCAGCTA CTCGGTGGGC CTCAATGAGG TGGAAGACCA CATCAAGAAC 
TACCGCCCCC AGTGCCTGGT GCTCACGGGG CCCCCCAACT TCCGCCCGGC CCTGGTGGAC 
TTTGTGGGCA CCTTCACCCG GAACCTCAGC CTGATGATCT GTGGCCACGT GCTCATCGGA 
CCCCACAAGC AGAGGATGCC TGAGCTCCAG CTCATCGCCA ACGGGCACAC CAAGTGGCTG 
AACAAGAGGA AGATCAAGGC CTTCTACTCG GATGTCATTG CCGAGGACCT CCGCAGAGGC 
GTCCAGATCC TCATGCAGGC CGCAGGTCTC GGGAGAATGA AGCCCAACAT TCTGGTGGTT 
GGGTTCAAGA AGAACTGGCA GTCGGCTCAC CCGGCCACAG TGGAAGACTA CATTGGCATC 
CTCCATGATG CCTTTGATTT CAACTATGGC GTGTGTGTCA TGAGGATGCG GGAGGGACTC 
AACGTGTCCA AGATGATGCA GGCGCACATT AACCCCGTGT TTGACCCAGC GGAGGACGGG 
AAGGAAGCCA GCGCCAGAGT GGACCCCAAG GCCCTGGTGA AGGAGGAGCA GGCCACCACC 
ATCTTCCAGT CGGAGCAGGG CAAGAAGACC ATAGACATCT ACTGGCTCTT TGACGATGGA 
GGCCTCACCC TCCTCATTCC CTATCTCCTT GGCCGCAAGA GGAGGTGGAG CAAATGCAAG 
ATCCGTGTGT TCGTAGGCGG CCAGATTAAC AGGATGGACC AGGAGAGAAA GGCGATCATT 
TCTCTGCTGA GCAAGTTCCG ACTGGGATTC CATGAAGTCC ACATCCTCCC TGACATCAAC 
CAGAACCCTC GGGCTGAGCA CACCAAGAGG TTTGAGGACA TGATTGCACC CTTCCGTCTG 
AATGATGGCT TCAAGGATGA GGCCACTGTC AACGAGATGC GGCGGGACTG CCCCTGGAAG 
ATCTCAGATG AGGAGATTAC GAAGAACAGA GTCAAGTCCC TTCGGCAGGT GAGGCTGAAT 
GAGATTGTGC TGGATTACTC CCGAGACGCT GCTCTCATCG TCATCACTTT GCCCATAGGG 
AGGAAGGGGA AGTGCCCCAG CTCGCTGTAC ATGGCCTGGC TGGAGACCCT GTCCCAGGAC 
CTCAGACCTC CAGTCATCCT GATCCGAGGA AACCAGGAAA ACGTGCTCAC CTTTTACTGC 
CAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A3 ( NM_001126107.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001119579.1 Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu26776
Clone ID Related Accession (Same CDS sequence)	NM_001126107.1
Accession Version	NM_001126107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3090bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-03
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 3 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK315298.1, U44128.1, AC012181.8 and BF510426.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region, compared to variant 1, resulting in a protein (isoform 2) that is 1 aa shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK315298.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1970526, SAMEA2145774 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGGAAGGCAG ACACCTGCAT 
GCCCTGGCCT TTGACAGCCG GCCCAGCCAC GAGATGACTG ATGGGCTGGT GGAGGGCGAG 
GCAGGCACCA GCAGCGAGAA GAACCCCGAG GAGCCAGTGC GCTTCGGCTG GGTCAAGGGG 
GTGATGATTC GTTGCATGCT CAACATTTGG GGCGTGATCC TCTACCTGCG GCTGCCCTGG 
ATTACGGCCC AGGCAGGCAT CGTCCTGACC TGGATCATCA TCCTGCTGTC GGTCACGGTG 
ACCTCCATCA CAGGCCTCTC CATCTCAGCC ATCTCCACCA ATGGCAAGGT CAAGTCAGGT 
GGCACCTACT TCCTCATCTC CCGGAGTCTG GGCCCAGAGC TTGGGGGCTC CATCGGCCTC 
ATTTTCGCTT TCGCCAATGC CGTGGGTGTG GCCATGCACA CGGTGGGCTT TGCAGAGACC 
GTGCGGGACC TGCTCCAGGA GTATGGGGCA CCCATCGTGG ACCCCATTAA CGACATCCGC 
ATCATTGGCG TGGTCTCGGT CACTGTGCTG CTGGCCATCT CCCTGGCTGG CATGGAGTGG 
GAGTCCAAGG CCCAGGTGCT GTTCTTCCTT GTCATCATGG TCTCCTTTGC CAACTATTTA 
GTGGGGACGC TGATCCCCCC ATCTGAGGAC AAGGCCTCCA AAGGCTTCTT CAGCTACCGG 
GCGGACATTT TTGTCCAGAA CTTGGTGCCT GACTGGCGGG GTCCAGATGG CACCTTCTTC 
GGAATGTTCT CCATCTTCTT CCCCTCGGCC ACAGGCATCC TGGCAGGGGC CAACATATCT 
GGTGACCTCA AGGACCCTGC TATAGCCATC CCCAAGGGGA CCCTCATGGC CATTTTCTGG 
ACGACCATTT CCTACCTGGC CATCTCAGCC ACCATTGGCT CCTGCGTGGT GCGTGATGCC 
TCTGGGGTCC TGAATGACAC AGTGACCCCT GGCTGGGGTG CCTGCGAGGG GCTGGCCTGC 
AGCTATGGCT GGAACTTCAC CGAGTGCACC CAGCAGCACA GCTGCCACTA CGGCCTCATC 
AACTATTACC AGACCATGAG CATGGTGTCA GGCTTCGCGC CCCTGATCAC GGCTGGCATC 
TTCGGGGCCA CCCTCTCCTC TGCCCTGGCC TGCCTTGTCT CTGCTGCCAA AGTCTTCCAG 
TGCCTTTGCG AGGACCAGCT GTACCCACTG ATCGGCTTCT TCGGCAAAGG CTATGGCAAG 
AACAAGGAGC CCGTGCGTGG CTACCTGCTG GCCTACGCCA TCGCTGTGGC CTTCATCATC 
ATCGCTGAGC TCAACACCAT AGCCCCCATC ATTTCCAACT TCTTCCTCTG CTCCTATGCC 
CTCATCAACT TCAGCTGCTT CCACGCCTCC ATCACCAACT CGCCTGGGTG GAGACCTTCA 
TTCCAATACT ACAACAAGTG GGCGGCGCTG TTTGGGGCTA TCATCTCCGT GGTCATCATG 
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATTGGCG TGGTGCTCTT CCTCCTGCTC 
TATGTCATCT ACAAGAAGCC AGAGGTAAAT TGGGGCTCCT CGGTACAGGC TGGCTCCTAC 
AACCTGGCCC TCAGCTACTC GGTGGGCCTC AATGAGGTGG AAGACCACAT CAAGAACTAC 
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTT 
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCACGTGCT CATCGGACCC 
CACAAGCAGA GGATGCCTGA GCTCCAGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC 
AAGAGGAAGA TCAAGGCCTT CTACTCGGAT GTCATTGCCG AGGACCTCCG CAGAGGCGTC 
CAGATCCTCA TGCAGGCCGC AGGTCTCGGG AGAATGAAGC CCAACATTCT GGTGGTTGGG 
TTCAAGAAGA ACTGGCAGTC GGCTCACCCG GCCACAGTGG AAGACTACAT TGGCATCCTC 
CATGATGCCT TTGATTTCAA CTATGGCGTG TGTGTCATGA GGATGCGGGA GGGACTCAAC 
GTGTCCAAGA TGATGCAGGC GCACATTAAC CCCGTGTTTG ACCCAGCGGA GGACGGGAAG 
GAAGCCAGCG CCAGAGGTGC CAGGCCATCA GTCTCTGGCG CTTTGGACCC CAAGGCCCTG 
GTGAAGGAGG AGCAGGCCAC CACCATCTTC CAGTCGGAGC AGGGCAAGAA GACCATAGAC 
ATCTACTGGC TCTTTGACGA TGGAGGCCTC ACCCTCCTCA TTCCCTATCT CCTTGGCCGC 
AAGAGGAGGT GGAGCAAATG CAAGATCCGT GTGTTCGTAG GCGGCCAGAT TAACAGGATG 
GACCAGGAGA GAAAGGCGAT CATTTCTCTG CTGAGCAAGT TCCGACTGGG ATTCCATGAA 
GTCCACATCC TCCCTGACAT CAACCAGAAC CCTCGGGCTG AGCACACCAA GAGGTTTGAG 
GACATGATTG CACCCTTCCG TCTGAATGAT GGCTTCAAGG ATGAGGCCAC TGTCAACGAG 
ATGCGGCGGG ACTGCCCCTG GAAGATCTCA GATGAGGAGA TTACGAAGAA CAGAGTCAAG 
TCCCTTCGGC AGGTGAGGCT GAATGAGATT GTGCTGGATT ACTCCCGAGA CGCTGCTCTC 
ATCGTCATCA CTTTGCCCAT AGGGAGGAAG GGGAAGTGCC CCAGCTCGCT GTACATGGCC 
TGGCTGGAGA CCCTGTCCCA GGACCTCAGA CCTCCAGTCA TCCTGATCCG AGGAAACCAG 
GAAAACGTGC TCACCTTTTA CTGCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001119579.1
CDS	30..3119
Translation	MAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSHPSHLTHSSTFCMRTFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHSFLKEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPINDIRIIGVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPAEDGKEASARGARPSVSGALDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	NM_001126107.1
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001126107.1

ORF Insert Sequence:

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGGAAGGCAG ACACCTGCAT 
GCCCTGGCCT TTGACAGCCG GCCCAGCCAC GAGATGACTG ATGGGCTGGT GGAGGGCGAG 
GCAGGCACCA GCAGCGAGAA GAACCCCGAG GAGCCAGTGC GCTTCGGCTG GGTCAAGGGG 
GTGATGATTC GTTGCATGCT CAACATTTGG GGCGTGATCC TCTACCTGCG GCTGCCCTGG 
ATTACGGCCC AGGCAGGCAT CGTCCTGACC TGGATCATCA TCCTGCTGTC GGTCACGGTG 
ACCTCCATCA CAGGCCTCTC CATCTCAGCC ATCTCCACCA ATGGCAAGGT CAAGTCAGGT 
GGCACCTACT TCCTCATCTC CCGGAGTCTG GGCCCAGAGC TTGGGGGCTC CATCGGCCTC 
ATTTTCGCTT TCGCCAATGC CGTGGGTGTG GCCATGCACA CGGTGGGCTT TGCAGAGACC 
GTGCGGGACC TGCTCCAGGA GTATGGGGCA CCCATCGTGG ACCCCATTAA CGACATCCGC 
ATCATTGGCG TGGTCTCGGT CACTGTGCTG CTGGCCATCT CCCTGGCTGG CATGGAGTGG 
GAGTCCAAGG CCCAGGTGCT GTTCTTCCTT GTCATCATGG TCTCCTTTGC CAACTATTTA 
GTGGGGACGC TGATCCCCCC ATCTGAGGAC AAGGCCTCCA AAGGCTTCTT CAGCTACCGG 
GCGGACATTT TTGTCCAGAA CTTGGTGCCT GACTGGCGGG GTCCAGATGG CACCTTCTTC 
GGAATGTTCT CCATCTTCTT CCCCTCGGCC ACAGGCATCC TGGCAGGGGC CAACATATCT 
GGTGACCTCA AGGACCCTGC TATAGCCATC CCCAAGGGGA CCCTCATGGC CATTTTCTGG 
ACGACCATTT CCTACCTGGC CATCTCAGCC ACCATTGGCT CCTGCGTGGT GCGTGATGCC 
TCTGGGGTCC TGAATGACAC AGTGACCCCT GGCTGGGGTG CCTGCGAGGG GCTGGCCTGC 
AGCTATGGCT GGAACTTCAC CGAGTGCACC CAGCAGCACA GCTGCCACTA CGGCCTCATC 
AACTATTACC AGACCATGAG CATGGTGTCA GGCTTCGCGC CCCTGATCAC GGCTGGCATC 
TTCGGGGCCA CCCTCTCCTC TGCCCTGGCC TGCCTTGTCT CTGCTGCCAA AGTCTTCCAG 
TGCCTTTGCG AGGACCAGCT GTACCCACTG ATCGGCTTCT TCGGCAAAGG CTATGGCAAG 
AACAAGGAGC CCGTGCGTGG CTACCTGCTG GCCTACGCCA TCGCTGTGGC CTTCATCATC 
ATCGCTGAGC TCAACACCAT AGCCCCCATC ATTTCCAACT TCTTCCTCTG CTCCTATGCC 
CTCATCAACT TCAGCTGCTT CCACGCCTCC ATCACCAACT CGCCTGGGTG GAGACCTTCA 
TTCCAATACT ACAACAAGTG GGCGGCGCTG TTTGGGGCTA TCATCTCCGT GGTCATCATG 
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATTGGCG TGGTGCTCTT CCTCCTGCTC 
TATGTCATCT ACAAGAAGCC AGAGGTAAAT TGGGGCTCCT CGGTACAGGC TGGCTCCTAC 
AACCTGGCCC TCAGCTACTC GGTGGGCCTC AATGAGGTGG AAGACCACAT CAAGAACTAC 
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTT 
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCACGTGCT CATCGGACCC 
CACAAGCAGA GGATGCCTGA GCTCCAGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC 
AAGAGGAAGA TCAAGGCCTT CTACTCGGAT GTCATTGCCG AGGACCTCCG CAGAGGCGTC 
CAGATCCTCA TGCAGGCCGC AGGTCTCGGG AGAATGAAGC CCAACATTCT GGTGGTTGGG 
TTCAAGAAGA ACTGGCAGTC GGCTCACCCG GCCACAGTGG AAGACTACAT TGGCATCCTC 
CATGATGCCT TTGATTTCAA CTATGGCGTG TGTGTCATGA GGATGCGGGA GGGACTCAAC 
GTGTCCAAGA TGATGCAGGC GCACATTAAC CCCGTGTTTG ACCCAGCGGA GGACGGGAAG 
GAAGCCAGCG CCAGAGGTGC CAGGCCATCA GTCTCTGGCG CTTTGGACCC CAAGGCCCTG 
GTGAAGGAGG AGCAGGCCAC CACCATCTTC CAGTCGGAGC AGGGCAAGAA GACCATAGAC 
ATCTACTGGC TCTTTGACGA TGGAGGCCTC ACCCTCCTCA TTCCCTATCT CCTTGGCCGC 
AAGAGGAGGT GGAGCAAATG CAAGATCCGT GTGTTCGTAG GCGGCCAGAT TAACAGGATG 
GACCAGGAGA GAAAGGCGAT CATTTCTCTG CTGAGCAAGT TCCGACTGGG ATTCCATGAA 
GTCCACATCC TCCCTGACAT CAACCAGAAC CCTCGGGCTG AGCACACCAA GAGGTTTGAG 
GACATGATTG CACCCTTCCG TCTGAATGAT GGCTTCAAGG ATGAGGCCAC TGTCAACGAG 
ATGCGGCGGG ACTGCCCCTG GAAGATCTCA GATGAGGAGA TTACGAAGAA CAGAGTCAAG 
TCCCTTCGGC AGGTGAGGCT GAATGAGATT GTGCTGGATT ACTCCCGAGA CGCTGCTCTC 
ATCGTCATCA CTTTGCCCAT AGGGAGGAAG GGGAAGTGCC CCAGCTCGCT GTACATGGCC 
TGGCTGGAGA CCCTGTCCCA GGACCTCAGA CCTCCAGTCA TCCTGATCCG AGGAAACCAG 
GAAAACGTGC TCACCTTTTA CTGCCAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC12A3 ( XM_005256119.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005256176.1 Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant X1, mRNA.

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CloneID	OHu49480
Clone ID Related Accession (Same CDS sequence)	XM_005256119.2
Accession Version	XM_005256119.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	solute carrier family 12 member 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005256119.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGGAAGGCAG ACACCTGCAT 
GCCCTGGCCT TTGACAGCCG GCCCAGCCAC GAGATGACTG ATGGGCTGGT GGAGGGCGAG 
GCAGGCACCA GCAGCGAGAA GAACCCCGAG GAGCCAGTGC GCTTCGGCTG GGTCAAGGGG 
GTGATGATTC GTTGCATGCT CAACATTTGG GGCGTGATCC TCTACCTGCG GCTGCCCTGG 
ATTACGGCCC AGGCAGGCAT CGTCCTGACC TGGATCATCA TCCTGCTGTC GGTCACGGTG 
ACCTCCATCA CAGGCCTCTC CATCTCAGCC ATCTCCACCA ATGGCAAGGT CAAGTCAGGT 
GGCACCTACT TCCTCATCTC CCGGAGTCTG GGCCCAGAGC TTGGGGGCTC CATCGGCCTC 
ATTTTCGCTT TCGCCAATGC CGTGGGTGTG GCCATGCACA CGGTGGGCTT TGCAGAGACC 
GTGCGGGACC TGCTCCAGGA GTATGGGGCA CCCATCGTGG ACCCCATTAA CGACATCCGC 
ATCATTGCCG TGGTCTCGGT CACTGTGCTG CTGGCCATCT CCCTGGCTGG CATGGAGTGG 
GAGTCCAAGG CCCAGGTGCT GTTCTTCCTT GTCATCATGG TCTCCTTTGC CAACTATTTA 
GTGGGGACGC TGATCCCCCC ATCTGAGGAC AAGGCCTCCA AAGGCTTCTT CAGCTACCGG 
GCGGACATTT TTGTCCAGAA CTTGGTGCCT GACTGGCGGG GTCCAGATGG CACCTTCTTC 
GGAATGTTCT CCATCTTCTT CCCCTCGGCC ACAGGCATCC TGGCAGGGGC CAACATATCT 
GGTGACCTCA AGGACCCTGC TATAGCCATC CCCAAGGGGA CCCTCATGGC CATTTTCTGG 
ACGACCATTT CCTACCTGGC CATCTCAGCC ACCATTGGCT CCTGCGTGGT GCGTGATGCC 
TCTGGGGTCC TGAATGACAC AGTGACCCCT GGCTGGGGTG CCTGCGAGGG GCTGGCCTGC 
AGCTATGGCT GGAACTTCAC CGAGTGCACC CAGCAGCACA GCTGCCACTA CGGCCTCATC 
AACTATTACC AGACCATGAG CATGGTGTCA GGCTTCGCGC CCCTGATCAC GGCTGGCATC 
TTCGGGGCCA CCCTCTCCTC TGCCCTGGCC TGCCTTGTCT CTGCTGCCAA AGTCTTCCAG 
TGCCTTTGCG AGGACCAGCT GTACCCACTG ATCGGCTTCT TCGGCAAAGG CTATGGCAAG 
AACAAGGAGC CCGTGCGTGG CTACCTGCTG GCCTACGCCA TCGCTGTGGC CTTCATCATC 
ATCGCTGAGC TCAACACCAT AGCCCCCATC ATTTCCAACT TCTTCCTCTG CTCCTATGCC 
CTCATCAACT TCAGCTGCTT CCACGCCTCC ATCACCAACT CGCCTGGGTG GAGACCTTCA 
TTCCAATACT ACAACAAGTG GGCGGCGCTG TTTGGGGCTA TCATCTCCGT GGTCATCATG 
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATTGGCG TGGTGCTCTT CCTCCTGCTC 
TATGTCATCT ACAAGAAGCC AGAGGTAAAT TGGGGCTCCT CGGTACAGGC TGGCTCCTAC 
AACCTGGCCC TCAGCTACTC GGTGGGCCTC AATGAGGTGG AAGACCACAT CAAGAACTAC 
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTT 
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCACGTGCT CATCGGACCC 
CACAAGCAGA GGATGCCTGA GCTCCAGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC 
AAGAGGAAGA TCAAGGCCTT CTACTCGGAT GTCATTGCCG AGGACCTCCG CAGAGGCGTC 
CAGATCCTCA TGCAGGCCGC AGGTCTCGGG AGAATGAAGC CCAACATTCT GGTGGTTGGG 
TTCAAGAAGA ACTGGCAGTC GGCTCACCCG GCCACAGTGG AAGACTACAT TGGCATCCTC 
CATGATGCCT TTGATTTCAA CTATGGCGTG TGTGTCATGA GGATGCGGGA GGGACTCAAC 
GTGTCCAAGA TGATGCAGGC GCACATTAAC CCCGTGTTTG ACCCAGCGGA GGACGGGAAG 
GAAGCCAGCG CCAGAGTGGA CCCCAAGGCC CTGGTGAAGG AGGAGCAGGC CACCACCATC 
TTCCAGTCGG AGCAGGGCAA GAAGACCATA GACATCTACT GGCTCTTTGA CGATGGAGGC 
CTCACCCTCC TCATTCCCTA TCTCCTTGGC CGCAAGAGGA GGTGGAGCAA ATGCAAGATC 
CGTGTGTTCG TAGGCGGCCA GATTAACAGG ATGGACCAGG AGAGAAAGGC GATCATTTCT 
CTGCTGAGCA AGTTCCGACT GGGATTCCAT GAAGTCCACA TCCTCCCTGA CATCAACCAG 
AACCCTCGGG CTGAGCACAC CAAGAGGTTT GAGGACATGA TTGCACCCTT CCGTCTGAAT 
GATGGCTTCA AGGATGAGGC CACTGTCAAC GAGATGCGGC GGGACTGCCC CTGGAAGATC 
TCAGATGAGG AGATTACGAA GAACAGAGTC AAGTCCCTTC GGCAGGTGAG GCTGAATGAG 
ATTGTGCTGG ATTACTCCCG AGACGCTGCT CTCATCGTCA TCACTTTGCC CATAGGGAGG 
AAGGGGAAGT GCCCCAGCTC GCTGTACATG GCCTGGCTGG AGACCCTGTC CCAGGACCTC 
AGACCTCCAG TCATCCTGAT CCGAGGAAAC CAGGAAAACG TGCTCACCTT TTACTGCCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005256176.1
CDS	30..3092
Translation	MAELPTTETPGDATLCSGRFTISTLLSSDEPSPPAAYDSSHPSHLTHSSTFCMRTFGYNTIDVVPTYEHYANSTQPGEPRKVRPTLADLHSFLKEGRHLHALAFDSRPSHEMTDGLVEGEAGTSSEKNPEEPVRFGWVKGVMIRCMLNIWGVILYLRLPWITAQAGIVLTWIIILLSVTVTSITGLSISAISTNGKVKSGGTYFLISRSLGPELGGSIGLIFAFANAVGVAMHTVGFAETVRDLLQEYGAPIVDPINDIRIIAVVSVTVLLAISLAGMEWESKAQVLFFLVIMVSFANYLVGTLIPPSEDKASKGFFSYRADIFVQNLVPDWRGPDGTFFGMFSIFFPSATGILAGANISGDLKDPAIAIPKGTLMAIFWTTISYLAISATIGSCVVRDASGVLNDTVTPGWGACEGLACSYGWNFTECTQQHSCHYGLINYYQTMSMVSGFAPLITAGIFGATLSSALACLVSAAKVFQCLCEDQLYPLIGFFGKGYGKNKEPVRGYLLAYAIAVAFIIIAELNTIAPIISNFFLCSYALINFSCFHASITNSPGWRPSFQYYNKWAALFGAIISVVIMFLLTWWAALIAIGVVLFLLLYVIYKKPEVNWGSSVQAGSYNLALSYSVGLNEVEDHIKNYRPQCLVLTGPPNFRPALVDFVGTFTRNLSLMICGHVLIGPHKQRMPELQLIANGHTKWLNKRKIKAFYSDVIAEDLRRGVQILMQAAGLGRMKPNILVVGFKKNWQSAHPATVEDYIGILHDAFDFNYGVCVMRMREGLNVSKMMQAHINPVFDPAEDGKEASARVDPKALVKEEQATTIFQSEQGKKTIDIYWLFDDGGLTLLIPYLLGRKRRWSKCKIRVFVGGQINRMDQERKAIISLLSKFRLGFHEVHILPDINQNPRAEHTKRFEDMIAPFRLNDGFKDEATVNEMRRDCPWKISDEEITKNRVKSLRQVRLNEIVLDYSRDAALIVITLPIGRKGKCPSSLYMAWLETLSQDLRPPVILIRGNQENVLTFYCQ

Target ORF information:

RefSeq Version	XM_005256119.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 12 member 3 (SLC12A3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005256119.2

ORF Insert Sequence:

ATGGCAGAAC TGCCCACAAC AGAGACGCCT GGGGACGCCA CTTTGTGCAG CGGGCGCTTC 
ACCATCAGCA CACTGCTGAG CAGTGATGAG CCCTCTCCAC CAGCTGCCTA TGACAGCAGC 
CACCCCAGCC ACCTGACCCA CAGCAGCACC TTCTGCATGC GCACCTTTGG CTACAACACG 
ATCGATGTGG TGCCCACATA TGAGCACTAT GCCAACAGCA CCCAGCCTGG TGAGCCCCGG 
AAGGTCCGGC CCACACTGGC TGACCTGCAC TCCTTCCTCA AGGAAGGCAG ACACCTGCAT 
GCCCTGGCCT TTGACAGCCG GCCCAGCCAC GAGATGACTG ATGGGCTGGT GGAGGGCGAG 
GCAGGCACCA GCAGCGAGAA GAACCCCGAG GAGCCAGTGC GCTTCGGCTG GGTCAAGGGG 
GTGATGATTC GTTGCATGCT CAACATTTGG GGCGTGATCC TCTACCTGCG GCTGCCCTGG 
ATTACGGCCC AGGCAGGCAT CGTCCTGACC TGGATCATCA TCCTGCTGTC GGTCACGGTG 
ACCTCCATCA CAGGCCTCTC CATCTCAGCC ATCTCCACCA ATGGCAAGGT CAAGTCAGGT 
GGCACCTACT TCCTCATCTC CCGGAGTCTG GGCCCAGAGC TTGGGGGCTC CATCGGCCTC 
ATTTTCGCTT TCGCCAATGC CGTGGGTGTG GCCATGCACA CGGTGGGCTT TGCAGAGACC 
GTGCGGGACC TGCTCCAGGA GTATGGGGCA CCCATCGTGG ACCCCATTAA CGACATCCGC 
ATCATTGCCG TGGTCTCGGT CACTGTGCTG CTGGCCATCT CCCTGGCTGG CATGGAGTGG 
GAGTCCAAGG CCCAGGTGCT GTTCTTCCTT GTCATCATGG TCTCCTTTGC CAACTATTTA 
GTGGGGACGC TGATCCCCCC ATCTGAGGAC AAGGCCTCCA AAGGCTTCTT CAGCTACCGG 
GCGGACATTT TTGTCCAGAA CTTGGTGCCT GACTGGCGGG GTCCAGATGG CACCTTCTTC 
GGAATGTTCT CCATCTTCTT CCCCTCGGCC ACAGGCATCC TGGCAGGGGC CAACATATCT 
GGTGACCTCA AGGACCCTGC TATAGCCATC CCCAAGGGGA CCCTCATGGC CATTTTCTGG 
ACGACCATTT CCTACCTGGC CATCTCAGCC ACCATTGGCT CCTGCGTGGT GCGTGATGCC 
TCTGGGGTCC TGAATGACAC AGTGACCCCT GGCTGGGGTG CCTGCGAGGG GCTGGCCTGC 
AGCTATGGCT GGAACTTCAC CGAGTGCACC CAGCAGCACA GCTGCCACTA CGGCCTCATC 
AACTATTACC AGACCATGAG CATGGTGTCA GGCTTCGCGC CCCTGATCAC GGCTGGCATC 
TTCGGGGCCA CCCTCTCCTC TGCCCTGGCC TGCCTTGTCT CTGCTGCCAA AGTCTTCCAG 
TGCCTTTGCG AGGACCAGCT GTACCCACTG ATCGGCTTCT TCGGCAAAGG CTATGGCAAG 
AACAAGGAGC CCGTGCGTGG CTACCTGCTG GCCTACGCCA TCGCTGTGGC CTTCATCATC 
ATCGCTGAGC TCAACACCAT AGCCCCCATC ATTTCCAACT TCTTCCTCTG CTCCTATGCC 
CTCATCAACT TCAGCTGCTT CCACGCCTCC ATCACCAACT CGCCTGGGTG GAGACCTTCA 
TTCCAATACT ACAACAAGTG GGCGGCGCTG TTTGGGGCTA TCATCTCCGT GGTCATCATG 
TTCCTCCTCA CCTGGTGGGC GGCCCTCATC GCCATTGGCG TGGTGCTCTT CCTCCTGCTC 
TATGTCATCT ACAAGAAGCC AGAGGTAAAT TGGGGCTCCT CGGTACAGGC TGGCTCCTAC 
AACCTGGCCC TCAGCTACTC GGTGGGCCTC AATGAGGTGG AAGACCACAT CAAGAACTAC 
CGCCCCCAGT GCCTGGTGCT CACGGGGCCC CCCAACTTCC GCCCGGCCCT GGTGGACTTT 
GTGGGCACCT TCACCCGGAA CCTCAGCCTG ATGATCTGTG GCCACGTGCT CATCGGACCC 
CACAAGCAGA GGATGCCTGA GCTCCAGCTC ATCGCCAACG GGCACACCAA GTGGCTGAAC 
AAGAGGAAGA TCAAGGCCTT CTACTCGGAT GTCATTGCCG AGGACCTCCG CAGAGGCGTC 
CAGATCCTCA TGCAGGCCGC AGGTCTCGGG AGAATGAAGC CCAACATTCT GGTGGTTGGG 
TTCAAGAAGA ACTGGCAGTC GGCTCACCCG GCCACAGTGG AAGACTACAT TGGCATCCTC 
CATGATGCCT TTGATTTCAA CTATGGCGTG TGTGTCATGA GGATGCGGGA GGGACTCAAC 
GTGTCCAAGA TGATGCAGGC GCACATTAAC CCCGTGTTTG ACCCAGCGGA GGACGGGAAG 
GAAGCCAGCG CCAGAGTGGA CCCCAAGGCC CTGGTGAAGG AGGAGCAGGC CACCACCATC 
TTCCAGTCGG AGCAGGGCAA GAAGACCATA GACATCTACT GGCTCTTTGA CGATGGAGGC 
CTCACCCTCC TCATTCCCTA TCTCCTTGGC CGCAAGAGGA GGTGGAGCAA ATGCAAGATC 
CGTGTGTTCG TAGGCGGCCA GATTAACAGG ATGGACCAGG AGAGAAAGGC GATCATTTCT 
CTGCTGAGCA AGTTCCGACT GGGATTCCAT GAAGTCCACA TCCTCCCTGA CATCAACCAG 
AACCCTCGGG CTGAGCACAC CAAGAGGTTT GAGGACATGA TTGCACCCTT CCGTCTGAAT 
GATGGCTTCA AGGATGAGGC CACTGTCAAC GAGATGCGGC GGGACTGCCC CTGGAAGATC 
TCAGATGAGG AGATTACGAA GAACAGAGTC AAGTCCCTTC GGCAGGTGAG GCTGAATGAG 
ATTGTGCTGG ATTACTCCCG AGACGCTGCT CTCATCGTCA TCACTTTGCC CATAGGGAGG 
AAGGGGAAGT GCCCCAGCTC GCTGTACATG GCCTGGCTGG AGACCCTGTC CCAGGACCTC 
AGACCTCCAG TCATCCTGAT CCGAGGAAAC CAGGAAAACG TGCTCACCTT TTACTGCCAG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SLC12A3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

オンラインフォーム

サポート

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