SLC3A1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SLC3A1
Entrez Gene ID	6519
Full Name	solute carrier family 3 member 1
Synonyms	ATR1,CSNU1,D2H,NBAT,RBAT
General protein information	Preferred Names solute carrier family 3 member 1 Names neutral and basic amino acid transport protein rBAT B(0,+)-type amino acid transport protein SLC3A1 variant B SLC3A1 variant C SLC3A1 variant D SLC3A1 variant E SLC3A1 variant F SLC3A1 variant G amino acid transporter 1 solute carrier family 3 (amino acid transporter heavy chain), member 1 solute carrier family 3 (cystine, dibasic and neutral amino acid transporters), member 1 solute carrier family 3 (cystine, dibasic and neutral amino acid transporters, activator of cystine, dibasic and neutral amino acid transport), member 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p21
Summary	This gene encodes a type II membrane glycoprotein which is one of the components of the renal amino acid transporter which transports neutral and basic amino acids in the renal tubule and intestinal tract. Mutations and deletions in this gene are associated with cystinuria. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008].
Disorder MIM:	104614
Disorder Html:	Cystinuria, 220100 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000341.3	NP_000332.2	neutral and basic amino acid transport protein rBAT
XM_011533047.2	XP_011531349.1	neutral and basic amino acid transport protein rBAT isoform X1
NM_000341.4	NP_000332.2	neutral and basic amino acid transport protein rBAT
XM_011533047.3	XP_011531349.1	neutral and basic amino acid transport protein rBAT isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	SLC3A1	NP_000332.2
Danio rerio (zebrafish)	slc3a1	XP_685969.3
Bos taurus (cattle)	SLC3A1	NP_001029805.1
Mus musculus (house mouse)	Slc3a1	NP_033231.2
Gallus gallus (chicken)	SLC3A1	XP_426125.3
Neurospora crassa	NCU06523	XP_961795.1
Canis lupus familiaris (dog)	SLC3A1	NP_001003109.1
Drosophila melanogaster (fruit fly)	Mal-B1	NP_609522.1
Drosophila melanogaster (fruit fly)	Mal-B2	NP_609523.2
Pan troglodytes (chimpanzee)	SLC3A1	XP_515443.2
	SLC3A1	XP_001112327.2
Magnaporthe oryzae (rice blast fungus)	MGG_07547	XP_003711461.1
Xenopus tropicalis (tropical clawed frog)	slc3a1	NP_001072903.1
Rattus norvegicus (Norway rat)	Slc3a1	NP_058912.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008965	XP_319713.4

Gene Ontology
Bibliography

Related articles in PubMed

Clinical and genetic characterization of Chinese pediatric cystine stone patients.
Shen L, Cong X, Zhang X, Wang N, Zhou P, Xu Y, Zhu Q, Gu X
Journal of pediatric urology13(6)629.e1-629.e5(2017 Dec)

Spectrum of mutations in cystinuria patients presenting with prenatal hyperechoic colon.
Tostivint I, Royer N, Nicolas M, Bourillon A, Czerkiewicz I, Becker PH, Muller F, Benoist JF
Clinical genetics92(6)632-638(2017 Dec)

Associating mutations causing cystinuria with disease severity with the aim of providing precision medicine.
Martell HJ, Wong KA, Martin JF, Kassam Z, Thomas K, Wass MN
BMC genomics18(Suppl 5)550(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In a Saudi Arabian cohort of patients with cystinuria, two new variants in the SLC3A1 and SLC9A7 genes were discovered. All of the detected mutations were missense variants in three different exons, such as c.1711 T > A (p.Cys571Ser) (exon 10), c.1166C > T p.Thr389Met (exon 11) and c.1400 T > A p.Met467Lys (exon 8).
Title: Delineation of cystinuria in Saudi Arabia: A case series.

Here, the authors report that the expression level of the solute carrier family 3, member 1 (SLC3A1), the cysteine carrier, tightly correlated with clinical stages and patients' survival. Elevated SLC3A1 expression accelerated the cysteine uptake and the accumulation of reductive glutathione (GSH), leading to reduced reactive oxygen species (ROS).
Title: Cysteine transporter SLC3A1 promotes breast cancer tumorigenesis.

Deletion of the rBAT C-terminal disulfide loop (residues 673-685) prevented maturation and prompted degradation of the transporter.
Title: The role of N-glycans and the C-terminal loop of the subunit rBAT in the biogenesis of the cystinuria-associated transporter.

We found a novel mutation in the acceptor region in exon 1 (SLC3A1 gene) and detected a heterozygosity status for the described patients.
Title: A Novel Mutation in SLC3A1 Gene in Patients With Cystinuria.

Five SLC3A1 and SLC7A9 mutations appear to be responsible for the genetic basis of cystinuria in the Greek-Cypriot patients
Title: Molecular and Clinical Investigation of Cystinuria in the Greek-Cypriot Population.

The following SLC3A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SLC3A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19170	NM_000341.4 Latest version!	Homo sapiens solute carrier family 3 member 1 (SLC3A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu60547	XM_011533047.3 Latest version!	Homo sapiens solute carrier family 3 member 1 (SLC3A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
View Old Accession Versions >>
OHu19170	NM_000341.3	Homo sapiens solute carrier family 3 member 1 (SLC3A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu60547	XM_011533047.2	Homo sapiens solute carrier family 3 member 1 (SLC3A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$307.30 $439.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SLC3A1 ( NM_000341.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000332.2 Homo sapiens solute carrier family 3 member 1 (SLC3A1), mRNA.

Price & Availability↑

CloneID	OHu19170
Clone ID Related Accession (Same CDS sequence)	NM_000341.3 , NM_000341.4
Accession Version	NM_000341.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-17
Organism	Homo sapiens(human)
Product	neutral and basic amino acid transport protein rBAT
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC013717.8, BC093626.1 and AK223146.1. This sequence is a reference standard in the RefSeqGene project. On May 2, 2008 this sequence version replaced NM_000341.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB033549.1, BC022386.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTGAAG ATAAAAGCAA GAGAGACTCC ATCGAGATGA GTATGAAGGG ATGCCAGACA 
AACAACGGGT TTGTCCATAA TGAAGACATT CTGGAGCAGA CCCCGGATCC AGGAAGCTCA 
ACAGACAACC TGAAGCACAG CACCAGGGGC ATCCTTGGCT CCCAGGAGCC CGACTTCAAG 
GGCGTCCAGC CCTATGCGGG GATGCCCAAG GAGGTGCTGT TCCAGTTCTC TGGCCAGGCC 
CGCTACCGCA TACCTCGGGA GATCCTCTTC TGGCTCACAG TGGCTTCTGT GCTGGTGCTC 
ATCGCGGCCA CCATAGCCAT CATTGCCCTC TCTCCAAAGT GCCTAGACTG GTGGCAGGAG 
GGGCCCATGT ACCAGATCTA CCCAAGGTCT TTCAAGGACA GTAACAAGGA TGGGAACGGA 
GATCTGAAAG GTATTCAAGA TAAACTGGAC TACATCACAG CTTTAAATAT AAAAACTGTT 
TGGATTACTT CATTTTATAA ATCGTCCCTT AAAGATTTCA GATATGGTGT TGAAGATTTC 
CGGGAAGTTG ATCCCATTTT TGGAACGATG GAAGATTTTG AGAATCTGGT TGCAGCCATA 
CATGATAAAG GTTTAAAATT AATCATCGAT TTCATACCAA ACCACACGAG TGATAAACAT 
ATTTGGTTTC AATTGAGTCG GACACGGACA GGAAAATATA CTGATTATTA TATCTGGCAT 
GACTGTACCC ATGAAAATGG CAAAACCATT CCACCCAACA ACTGGTTAAG TGTGTATGGA 
AACTCCAGTT GGCACTTTGA CGAAGTGCGA AACCAATGTT ATTTTCATCA GTTTATGAAA 
GAGCAACCTG ATTTAAATTT CCGCAATCCT GATGTTCAAG AAGAAATAAA AGAAATTTTA 
CGGTTCTGGC TCACAAAGGG TGTTGATGGT TTTAGTTTGG ATGCTGTTAA ATTCCTCCTA 
GAAGCAAAGC ACCTGAGAGA TGAGATCCAA GTAAATAAGA CCCAAATCCC GGACACGGTC 
ACACAATACT CGGAGCTGTA CCATGACTTC ACCACCACGC AGGTGGGAAT GCACGACATT 
GTCCGCAGCT TCCGGCAGAC CATGGACCAA TACAGCACGG AGCCCGGCAG ATACAGGTTC 
ATGGGGACTG AAGCCTATGC AGAGAGTATT GACAGGACCG TGATGTACTA TGGATTGCCA 
TTTATCCAAG AAGCTGATTT TCCCTTCAAC AATTACCTCA GCATGCTAGA CACTGTTTCT 
GGGAACAGCG TGTATGAGGT TATCACATCC TGGATGGAAA ACATGCCAGA AGGAAAATGG 
CCTAACTGGA TGATTGGTGG ACCAGACAGT TCACGGCTGA CTTCGCGTTT GGGGAATCAG 
TATGTCAACG TGATGAACAT GCTTCTTTTC ACACTCCCTG GAACTCCTAT AACTTACTAT 
GGAGAAGAAA TTGGAATGGG AAATATTGTA GCCGCAAATC TCAATGAAAG CTATGATATT 
AATACCCTTC GCTCAAAGTC ACCAATGCAG TGGGACAATA GTTCAAATGC TGGTTTTTCT 
GAAGCTAGTA ACACCTGGTT ACCTACCAAT TCAGATTACC ACACTGTGAA TGTTGATGTC 
CAAAAGACTC AGCCCAGATC GGCTTTGAAG TTATATCAAG ATTTAAGTCT ACTTCATGCC 
AATGAGCTAC TCCTCAACAG GGGCTGGTTT TGCCATTTGA GGAATGACAG CCACTATGTT 
GTGTACACAA GAGAGCTGGA TGGCATCGAC AGAATCTTTA TCGTGGTTCT GAATTTTGGA 
GAATCAACAC TGTTAAATCT ACATAATATG ATTTCGGGCC TTCCCGCTAA AATGAGAATA 
AGGTTAAGTA CCAATTCTGC CGACAAAGGC AGTAAAGTTG ATACAAGTGG CATTTTTCTG 
GACAAGGGAG AGGGACTCAT CTTTGAACAC AACACGAAGA ATCTCCTTCA TCGCCAAACA 
GCTTTCAGAG ATAGATGCTT TGTTTCCAAT CGAGCATGCT ATTCCAGTGT ACTGAACATA 
CTGTATACCT CGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000332.2
CDS	79..2136
Translation	MAEDKSKRDSIEMSMKGCQTNNGFVHNEDILEQTPDPGSSTDNLKHSTRGILGSQEPDFKGVQPYAGMPKEVLFQFSGQARYRIPREILFWLTVASVLVLIAATIAIIALSPKCLDWWQEGPMYQIYPRSFKDSNKDGNGDLKGIQDKLDYITALNIKTVWITSFYKSSLKDFRYGVEDFREVDPIFGTMEDFENLVAAIHDKGLKLIIDFIPNHTSDKHIWFQLSRTRTGKYTDYYIWHDCTHENGKTIPPNNWLSVYGNSSWHFDEVRNQCYFHQFMKEQPDLNFRNPDVQEEIKEILRFWLTKGVDGFSLDAVKFLLEAKHLRDEIQVNKTQIPDTVTQYSELYHDFTTTQVGMHDIVRSFRQTMDQYSTEPGRYRFMGTEAYAESIDRTVMYYGLPFIQEADFPFNNYLSMLDTVSGNSVYEVITSWMENMPEGKWPNWMIGGPDSSRLTSRLGNQYVNVMNMLLFTLPGTPITYYGEEIGMGNIVAANLNESYDINTLRSKSPMQWDNSSNAGFSEASNTWLPTNSDYHTVNVDVQKTQPRSALKLYQDLSLLHANELLLNRGWFCHLRNDSHYVVYTRELDGIDRIFIVVLNFGESTLLNLHNMISGLPAKMRIRLSTNSADKGSKVDTSGIFLDKGEGLIFEHNTKNLLHRQTAFRDRCFVSNRACYSSVLNILYTSC

Target ORF information:

RefSeq Version	NM_000341.3
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 3 member 1 (SLC3A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000341.3

ORF Insert Sequence:

ATGGCTGAAG ATAAAAGCAA GAGAGACTCC ATCGAGATGA GTATGAAGGG ATGCCAGACA 
AACAACGGGT TTGTCCATAA TGAAGACATT CTGGAGCAGA CCCCGGATCC AGGAAGCTCA 
ACAGACAACC TGAAGCACAG CACCAGGGGC ATCCTTGGCT CCCAGGAGCC CGACTTCAAG 
GGCGTCCAGC CCTATGCGGG GATGCCCAAG GAGGTGCTGT TCCAGTTCTC TGGCCAGGCC 
CGCTACCGCA TACCTCGGGA GATCCTCTTC TGGCTCACAG TGGCTTCTGT GCTGGTGCTC 
ATCGCGGCCA CCATAGCCAT CATTGCCCTC TCTCCAAAGT GCCTAGACTG GTGGCAGGAG 
GGGCCCATGT ACCAGATCTA CCCAAGGTCT TTCAAGGACA GTAACAAGGA TGGGAACGGA 
GATCTGAAAG GTATTCAAGA TAAACTGGAC TACATCACAG CTTTAAATAT AAAAACTGTT 
TGGATTACTT CATTTTATAA ATCGTCCCTT AAAGATTTCA GATATGGTGT TGAAGATTTC 
CGGGAAGTTG ATCCCATTTT TGGAACGATG GAAGATTTTG AGAATCTGGT TGCAGCCATA 
CATGATAAAG GTTTAAAATT AATCATCGAT TTCATACCAA ACCACACGAG TGATAAACAT 
ATTTGGTTTC AATTGAGTCG GACACGGACA GGAAAATATA CTGATTATTA TATCTGGCAT 
GACTGTACCC ATGAAAATGG CAAAACCATT CCACCCAACA ACTGGTTAAG TGTGTATGGA 
AACTCCAGTT GGCACTTTGA CGAAGTGCGA AACCAATGTT ATTTTCATCA GTTTATGAAA 
GAGCAACCTG ATTTAAATTT CCGCAATCCT GATGTTCAAG AAGAAATAAA AGAAATTTTA 
CGGTTCTGGC TCACAAAGGG TGTTGATGGT TTTAGTTTGG ATGCTGTTAA ATTCCTCCTA 
GAAGCAAAGC ACCTGAGAGA TGAGATCCAA GTAAATAAGA CCCAAATCCC GGACACGGTC 
ACACAATACT CGGAGCTGTA CCATGACTTC ACCACCACGC AGGTGGGAAT GCACGACATT 
GTCCGCAGCT TCCGGCAGAC CATGGACCAA TACAGCACGG AGCCCGGCAG ATACAGGTTC 
ATGGGGACTG AAGCCTATGC AGAGAGTATT GACAGGACCG TGATGTACTA TGGATTGCCA 
TTTATCCAAG AAGCTGATTT TCCCTTCAAC AATTACCTCA GCATGCTAGA CACTGTTTCT 
GGGAACAGCG TGTATGAGGT TATCACATCC TGGATGGAAA ACATGCCAGA AGGAAAATGG 
CCTAACTGGA TGATTGGTGG ACCAGACAGT TCACGGCTGA CTTCGCGTTT GGGGAATCAG 
TATGTCAACG TGATGAACAT GCTTCTTTTC ACACTCCCTG GAACTCCTAT AACTTACTAT 
GGAGAAGAAA TTGGAATGGG AAATATTGTA GCCGCAAATC TCAATGAAAG CTATGATATT 
AATACCCTTC GCTCAAAGTC ACCAATGCAG TGGGACAATA GTTCAAATGC TGGTTTTTCT 
GAAGCTAGTA ACACCTGGTT ACCTACCAAT TCAGATTACC ACACTGTGAA TGTTGATGTC 
CAAAAGACTC AGCCCAGATC GGCTTTGAAG TTATATCAAG ATTTAAGTCT ACTTCATGCC 
AATGAGCTAC TCCTCAACAG GGGCTGGTTT TGCCATTTGA GGAATGACAG CCACTATGTT 
GTGTACACAA GAGAGCTGGA TGGCATCGAC AGAATCTTTA TCGTGGTTCT GAATTTTGGA 
GAATCAACAC TGTTAAATCT ACATAATATG ATTTCGGGCC TTCCCGCTAA AATGAGAATA 
AGGTTAAGTA CCAATTCTGC CGACAAAGGC AGTAAAGTTG ATACAAGTGG CATTTTTCTG 
GACAAGGGAG AGGGACTCAT CTTTGAACAC AACACGAAGA ATCTCCTTCA TCGCCAAACA 
GCTTTCAGAG ATAGATGCTT TGTTTCCAAT CGAGCATGCT ATTCCAGTGT ACTGAACATA 
CTGTATACCT CGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC3A1 ( NM_000341.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_000332.2 Homo sapiens solute carrier family 3 member 1 (SLC3A1), mRNA.

Price & Availability↑

CloneID	OHu19170
Clone ID Related Accession (Same CDS sequence)	NM_000341.3 , NM_000341.4
Accession Version	NM_000341.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2058bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-25
Organism	Homo sapiens(human)
Product	neutral and basic amino acid transport protein rBAT
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC013717.8, BC093626.1 and AK223146.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_000341.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK289636.1, L11696.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1970526 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000260649.11/ ENSP00000260649.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGAAG ATAAAAGCAA GAGAGACTCC ATCGAGATGA GTATGAAGGG ATGCCAGACA 
AACAACGGGT TTGTCCATAA TGAAGACATT CTGGAGCAGA CCCCGGATCC AGGAAGCTCA 
ACAGACAACC TGAAGCACAG CACCAGGGGC ATCCTTGGCT CCCAGGAGCC CGACTTCAAG 
GGCGTCCAGC CCTATGCGGG GATGCCCAAG GAGGTGCTGT TCCAGTTCTC TGGCCAGGCC 
CGCTACCGCA TACCTCGGGA GATCCTCTTC TGGCTCACAG TGGCTTCTGT GCTGGTGCTC 
ATCGCGGCCA CCATAGCCAT CATTGCCCTC TCTCCAAAGT GCCTAGACTG GTGGCAGGAG 
GGGCCCATGT ACCAGATCTA CCCAAGGTCT TTCAAGGACA GTAACAAGGA TGGGAACGGA 
GATCTGAAAG GTATTCAAGA TAAACTGGAC TACATCACAG CTTTAAATAT AAAAACTGTT 
TGGATTACTT CATTTTATAA ATCGTCCCTT AAAGATTTCA GATATGGTGT TGAAGATTTC 
CGGGAAGTTG ATCCCATTTT TGGAACGATG GAAGATTTTG AGAATCTGGT TGCAGCCATA 
CATGATAAAG GTTTAAAATT AATCATCGAT TTCATACCAA ACCACACGAG TGATAAACAT 
ATTTGGTTTC AATTGAGTCG GACACGGACA GGAAAATATA CTGATTATTA TATCTGGCAT 
GACTGTACCC ATGAAAATGG CAAAACCATT CCACCCAACA ACTGGTTAAG TGTGTATGGA 
AACTCCAGTT GGCACTTTGA CGAAGTGCGA AACCAATGTT ATTTTCATCA GTTTATGAAA 
GAGCAACCTG ATTTAAATTT CCGCAATCCT GATGTTCAAG AAGAAATAAA AGAAATTTTA 
CGGTTCTGGC TCACAAAGGG TGTTGATGGT TTTAGTTTGG ATGCTGTTAA ATTCCTCCTA 
GAAGCAAAGC ACCTGAGAGA TGAGATCCAA GTAAATAAGA CCCAAATCCC GGACACGGTC 
ACACAATACT CGGAGCTGTA CCATGACTTC ACCACCACGC AGGTGGGAAT GCACGACATT 
GTCCGCAGCT TCCGGCAGAC CATGGACCAA TACAGCACGG AGCCCGGCAG ATACAGGTTC 
ATGGGGACTG AAGCCTATGC AGAGAGTATT GACAGGACCG TGATGTACTA TGGATTGCCA 
TTTATCCAAG AAGCTGATTT TCCCTTCAAC AATTACCTCA GCATGCTAGA CACTGTTTCT 
GGGAACAGCG TGTATGAGGT TATCACATCC TGGATGGAAA ACATGCCAGA AGGAAAATGG 
CCTAACTGGA TGATTGGTGG ACCAGACAGT TCACGGCTGA CTTCGCGTTT GGGGAATCAG 
TATGTCAACG TGATGAACAT GCTTCTTTTC ACACTCCCTG GAACTCCTAT AACTTACTAT 
GGAGAAGAAA TTGGAATGGG AAATATTGTA GCCGCAAATC TCAATGAAAG CTATGATATT 
AATACCCTTC GCTCAAAGTC ACCAATGCAG TGGGACAATA GTTCAAATGC TGGTTTTTCT 
GAAGCTAGTA ACACCTGGTT ACCTACCAAT TCAGATTACC ACACTGTGAA TGTTGATGTC 
CAAAAGACTC AGCCCAGATC GGCTTTGAAG TTATATCAAG ATTTAAGTCT ACTTCATGCC 
AATGAGCTAC TCCTCAACAG GGGCTGGTTT TGCCATTTGA GGAATGACAG CCACTATGTT 
GTGTACACAA GAGAGCTGGA TGGCATCGAC AGAATCTTTA TCGTGGTTCT GAATTTTGGA 
GAATCAACAC TGTTAAATCT ACATAATATG ATTTCGGGCC TTCCCGCTAA AATGAGAATA 
AGGTTAAGTA CCAATTCTGC CGACAAAGGC AGTAAAGTTG ATACAAGTGG CATTTTTCTG 
GACAAGGGAG AGGGACTCAT CTTTGAACAC AACACGAAGA ATCTCCTTCA TCGCCAAACA 
GCTTTCAGAG ATAGATGCTT TGTTTCCAAT CGAGCATGCT ATTCCAGTGT ACTGAACATA 
CTGTATACCT CGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000332.2
CDS	57..2114
Translation	MAEDKSKRDSIEMSMKGCQTNNGFVHNEDILEQTPDPGSSTDNLKHSTRGILGSQEPDFKGVQPYAGMPKEVLFQFSGQARYRIPREILFWLTVASVLVLIAATIAIIALSPKCLDWWQEGPMYQIYPRSFKDSNKDGNGDLKGIQDKLDYITALNIKTVWITSFYKSSLKDFRYGVEDFREVDPIFGTMEDFENLVAAIHDKGLKLIIDFIPNHTSDKHIWFQLSRTRTGKYTDYYIWHDCTHENGKTIPPNNWLSVYGNSSWHFDEVRNQCYFHQFMKEQPDLNFRNPDVQEEIKEILRFWLTKGVDGFSLDAVKFLLEAKHLRDEIQVNKTQIPDTVTQYSELYHDFTTTQVGMHDIVRSFRQTMDQYSTEPGRYRFMGTEAYAESIDRTVMYYGLPFIQEADFPFNNYLSMLDTVSGNSVYEVITSWMENMPEGKWPNWMIGGPDSSRLTSRLGNQYVNVMNMLLFTLPGTPITYYGEEIGMGNIVAANLNESYDINTLRSKSPMQWDNSSNAGFSEASNTWLPTNSDYHTVNVDVQKTQPRSALKLYQDLSLLHANELLLNRGWFCHLRNDSHYVVYTRELDGIDRIFIVVLNFGESTLLNLHNMISGLPAKMRIRLSTNSADKGSKVDTSGIFLDKGEGLIFEHNTKNLLHRQTAFRDRCFVSNRACYSSVLNILYTSC

Target ORF information:

RefSeq Version	NM_000341.4
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 3 member 1 (SLC3A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000341.4

ORF Insert Sequence:

ATGGCTGAAG ATAAAAGCAA GAGAGACTCC ATCGAGATGA GTATGAAGGG ATGCCAGACA 
AACAACGGGT TTGTCCATAA TGAAGACATT CTGGAGCAGA CCCCGGATCC AGGAAGCTCA 
ACAGACAACC TGAAGCACAG CACCAGGGGC ATCCTTGGCT CCCAGGAGCC CGACTTCAAG 
GGCGTCCAGC CCTATGCGGG GATGCCCAAG GAGGTGCTGT TCCAGTTCTC TGGCCAGGCC 
CGCTACCGCA TACCTCGGGA GATCCTCTTC TGGCTCACAG TGGCTTCTGT GCTGGTGCTC 
ATCGCGGCCA CCATAGCCAT CATTGCCCTC TCTCCAAAGT GCCTAGACTG GTGGCAGGAG 
GGGCCCATGT ACCAGATCTA CCCAAGGTCT TTCAAGGACA GTAACAAGGA TGGGAACGGA 
GATCTGAAAG GTATTCAAGA TAAACTGGAC TACATCACAG CTTTAAATAT AAAAACTGTT 
TGGATTACTT CATTTTATAA ATCGTCCCTT AAAGATTTCA GATATGGTGT TGAAGATTTC 
CGGGAAGTTG ATCCCATTTT TGGAACGATG GAAGATTTTG AGAATCTGGT TGCAGCCATA 
CATGATAAAG GTTTAAAATT AATCATCGAT TTCATACCAA ACCACACGAG TGATAAACAT 
ATTTGGTTTC AATTGAGTCG GACACGGACA GGAAAATATA CTGATTATTA TATCTGGCAT 
GACTGTACCC ATGAAAATGG CAAAACCATT CCACCCAACA ACTGGTTAAG TGTGTATGGA 
AACTCCAGTT GGCACTTTGA CGAAGTGCGA AACCAATGTT ATTTTCATCA GTTTATGAAA 
GAGCAACCTG ATTTAAATTT CCGCAATCCT GATGTTCAAG AAGAAATAAA AGAAATTTTA 
CGGTTCTGGC TCACAAAGGG TGTTGATGGT TTTAGTTTGG ATGCTGTTAA ATTCCTCCTA 
GAAGCAAAGC ACCTGAGAGA TGAGATCCAA GTAAATAAGA CCCAAATCCC GGACACGGTC 
ACACAATACT CGGAGCTGTA CCATGACTTC ACCACCACGC AGGTGGGAAT GCACGACATT 
GTCCGCAGCT TCCGGCAGAC CATGGACCAA TACAGCACGG AGCCCGGCAG ATACAGGTTC 
ATGGGGACTG AAGCCTATGC AGAGAGTATT GACAGGACCG TGATGTACTA TGGATTGCCA 
TTTATCCAAG AAGCTGATTT TCCCTTCAAC AATTACCTCA GCATGCTAGA CACTGTTTCT 
GGGAACAGCG TGTATGAGGT TATCACATCC TGGATGGAAA ACATGCCAGA AGGAAAATGG 
CCTAACTGGA TGATTGGTGG ACCAGACAGT TCACGGCTGA CTTCGCGTTT GGGGAATCAG 
TATGTCAACG TGATGAACAT GCTTCTTTTC ACACTCCCTG GAACTCCTAT AACTTACTAT 
GGAGAAGAAA TTGGAATGGG AAATATTGTA GCCGCAAATC TCAATGAAAG CTATGATATT 
AATACCCTTC GCTCAAAGTC ACCAATGCAG TGGGACAATA GTTCAAATGC TGGTTTTTCT 
GAAGCTAGTA ACACCTGGTT ACCTACCAAT TCAGATTACC ACACTGTGAA TGTTGATGTC 
CAAAAGACTC AGCCCAGATC GGCTTTGAAG TTATATCAAG ATTTAAGTCT ACTTCATGCC 
AATGAGCTAC TCCTCAACAG GGGCTGGTTT TGCCATTTGA GGAATGACAG CCACTATGTT 
GTGTACACAA GAGAGCTGGA TGGCATCGAC AGAATCTTTA TCGTGGTTCT GAATTTTGGA 
GAATCAACAC TGTTAAATCT ACATAATATG ATTTCGGGCC TTCCCGCTAA AATGAGAATA 
AGGTTAAGTA CCAATTCTGC CGACAAAGGC AGTAAAGTTG ATACAAGTGG CATTTTTCTG 
GACAAGGGAG AGGGACTCAT CTTTGAACAC AACACGAAGA ATCTCCTTCA TCGCCAAACA 
GCTTTCAGAG ATAGATGCTT TGTTTCCAAT CGAGCATGCT ATTCCAGTGT ACTGAACATA 
CTGTATACCT CGTGTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC3A1 ( XM_011533047.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531349.1 Homo sapiens solute carrier family 3 member 1 (SLC3A1), transcript variant X1, mRNA.

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CloneID	OHu60547
Clone ID Related Accession (Same CDS sequence)	XM_011533047.2 , XM_011533047.3
Accession Version	XM_011533047.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	neutral and basic amino acid transport protein rBAT isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533047.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAAG ATAAAAGCAA GAGAGACTCC ATCGAGATGA GTATGAAGGG ATGCCAGACA 
AACAACGGGT TTGTCCATAA TGAAGACATT CTGGAGCAGA CCCCGGATCC AGGAAGCTCA 
ACAGACAACC TGAAGCACAG CACCAGGGGC ATCCTTGGCT CCCAGGAGCC CGACTTCAAG 
GGCGTCCAGC CCTATGCGGG GATGCCCAAG GAGGTGCTGT TCCAGTTCTC TGGCCAGGCC 
CGCTACCGCA TACCTCGGGA GATCCTCTTC TGGCTCACAG TGGCTTCTGT GCTGGTGCTC 
ATCGCGGCCA CCATAGCCAT CATTGCCCTC TCTCCAAAGT GCCTAGACTG GTGGCAGGAG 
GGGCCCATGT ACCAGATCTA CCCAAGGTCT TTCAAGGACA GTAACAAGGA TGGGAACGGA 
GATCTGAAAG GTATTCAAGA TAAACTGGAC TACATCACAG CTTTAAATAT AAAAACTGTT 
TGGATTACTT CATTTTATAA ATCGTCCCTT AAAGATTTCA GATATGGTGT TGAAGATTTC 
CGGGAAGTTG ATCCCATTTT TGGAACGATG GAAGATTTTG AGAATCTGGT TGCAGCCATA 
CATGATAAAG GTTTAAAATT AATCATCGAT TTCATACCAA ACCACACGAG TGATAAACAT 
ATTTGGTTTC AATTGAGTCG GACACGGACA GGAAAATATA CTGATTATTA TATCTGGCAT 
GACTGTACCC ATGAAAATGG CAAAACCATT CCACCCAACA ACTGGTTAAG TGTGTATGGA 
AACTCCAGTT GGCACTTTGA CGAAGTGCGA AACCAATGTT ATTTTCATCA GTTTATGAAA 
GAGCAACCTG ATTTAAATTT CCGCAATCCT GATGTTCAAG AAGAAATAAA AGAAATTTTA 
CGGTTCTGGC TCACAAAGGG TGTTGATGGT TTTAGTTTGG ATGCTGTTAA ATTCCTCCTA 
GAAGCAAAGC ACCTGAGAGA TGAGATCCAA GTAAATAAGA CCCAAATCCC GGACACGGTC 
ACACAATACT CGGAGCTGTA CCATGACTTC ACCACCACGC AGGTGGGAAT GCACGACATT 
GTCCGCAGCT TCCGGCAGAC CATGGACCAA TACAGCACGG AGCCCGGCAG ATACAGGTTC 
ATGGGGACTG AAGCCTATGC AGAGAGTATT GACAGGACCG TGATGTACTA TGGATTGCCA 
TTTATCCAAG AAGCTGATTT TCCCTTCAAC AATTACCTCA GCATGCTAGA CACTGTTTCT 
GGGAACAGCG TGTATGAGGT TATCACATCC TGGATGGAAA ACATGCCAGA AGGAAAATGG 
CCTAACTGGA TGATTGGTGG ACCAGACAGT TCACGGCTGA CTTCGCGTTT GGGGAATCAG 
TATGTCAACG TGATGAACAT GCTTCTTTTC ACACTCCCTG GAACTCCTAT AACTTACTAT 
GGAGAAGAAA TTGGAATGGG AAATATTGTA GCCGCAAATC TCAATGAAAG CTATGATATT 
AATACCCTTC GCTCAAAGTC ACCAATGCAG TGGGACAATA GTTCAAATGC TGGTTTTTCT 
GAAGCTAGTA ACACCTGGTT ACCTACCAAT TCAGATTACC ACACTGTGAA TGTTGATTCT 
TGCTCCGTCA CCCATGCTCG AGTGCAGTGG CGTGATCTCG GCACACTGCA GCCTCTGCTT 
CCTGGGTTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531349.1
CDS	79..1773
Translation	MAEDKSKRDSIEMSMKGCQTNNGFVHNEDILEQTPDPGSSTDNLKHSTRGILGSQEPDFKGVQPYAGMPKEVLFQFSGQARYRIPREILFWLTVASVLVLIAATIAIIALSPKCLDWWQEGPMYQIYPRSFKDSNKDGNGDLKGIQDKLDYITALNIKTVWITSFYKSSLKDFRYGVEDFREVDPIFGTMEDFENLVAAIHDKGLKLIIDFIPNHTSDKHIWFQLSRTRTGKYTDYYIWHDCTHENGKTIPPNNWLSVYGNSSWHFDEVRNQCYFHQFMKEQPDLNFRNPDVQEEIKEILRFWLTKGVDGFSLDAVKFLLEAKHLRDEIQVNKTQIPDTVTQYSELYHDFTTTQVGMHDIVRSFRQTMDQYSTEPGRYRFMGTEAYAESIDRTVMYYGLPFIQEADFPFNNYLSMLDTVSGNSVYEVITSWMENMPEGKWPNWMIGGPDSSRLTSRLGNQYVNVMNMLLFTLPGTPITYYGEEIGMGNIVAANLNESYDINTLRSKSPMQWDNSSNAGFSEASNTWLPTNSDYHTVNVDSCSVTHARVQWRDLGTLQPLLPGFK

Target ORF information:

RefSeq Version	XM_011533047.2
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 3 member 1 (SLC3A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533047.2

ORF Insert Sequence:

ATGGCTGAAG ATAAAAGCAA GAGAGACTCC ATCGAGATGA GTATGAAGGG ATGCCAGACA 
AACAACGGGT TTGTCCATAA TGAAGACATT CTGGAGCAGA CCCCGGATCC AGGAAGCTCA 
ACAGACAACC TGAAGCACAG CACCAGGGGC ATCCTTGGCT CCCAGGAGCC CGACTTCAAG 
GGCGTCCAGC CCTATGCGGG GATGCCCAAG GAGGTGCTGT TCCAGTTCTC TGGCCAGGCC 
CGCTACCGCA TACCTCGGGA GATCCTCTTC TGGCTCACAG TGGCTTCTGT GCTGGTGCTC 
ATCGCGGCCA CCATAGCCAT CATTGCCCTC TCTCCAAAGT GCCTAGACTG GTGGCAGGAG 
GGGCCCATGT ACCAGATCTA CCCAAGGTCT TTCAAGGACA GTAACAAGGA TGGGAACGGA 
GATCTGAAAG GTATTCAAGA TAAACTGGAC TACATCACAG CTTTAAATAT AAAAACTGTT 
TGGATTACTT CATTTTATAA ATCGTCCCTT AAAGATTTCA GATATGGTGT TGAAGATTTC 
CGGGAAGTTG ATCCCATTTT TGGAACGATG GAAGATTTTG AGAATCTGGT TGCAGCCATA 
CATGATAAAG GTTTAAAATT AATCATCGAT TTCATACCAA ACCACACGAG TGATAAACAT 
ATTTGGTTTC AATTGAGTCG GACACGGACA GGAAAATATA CTGATTATTA TATCTGGCAT 
GACTGTACCC ATGAAAATGG CAAAACCATT CCACCCAACA ACTGGTTAAG TGTGTATGGA 
AACTCCAGTT GGCACTTTGA CGAAGTGCGA AACCAATGTT ATTTTCATCA GTTTATGAAA 
GAGCAACCTG ATTTAAATTT CCGCAATCCT GATGTTCAAG AAGAAATAAA AGAAATTTTA 
CGGTTCTGGC TCACAAAGGG TGTTGATGGT TTTAGTTTGG ATGCTGTTAA ATTCCTCCTA 
GAAGCAAAGC ACCTGAGAGA TGAGATCCAA GTAAATAAGA CCCAAATCCC GGACACGGTC 
ACACAATACT CGGAGCTGTA CCATGACTTC ACCACCACGC AGGTGGGAAT GCACGACATT 
GTCCGCAGCT TCCGGCAGAC CATGGACCAA TACAGCACGG AGCCCGGCAG ATACAGGTTC 
ATGGGGACTG AAGCCTATGC AGAGAGTATT GACAGGACCG TGATGTACTA TGGATTGCCA 
TTTATCCAAG AAGCTGATTT TCCCTTCAAC AATTACCTCA GCATGCTAGA CACTGTTTCT 
GGGAACAGCG TGTATGAGGT TATCACATCC TGGATGGAAA ACATGCCAGA AGGAAAATGG 
CCTAACTGGA TGATTGGTGG ACCAGACAGT TCACGGCTGA CTTCGCGTTT GGGGAATCAG 
TATGTCAACG TGATGAACAT GCTTCTTTTC ACACTCCCTG GAACTCCTAT AACTTACTAT 
GGAGAAGAAA TTGGAATGGG AAATATTGTA GCCGCAAATC TCAATGAAAG CTATGATATT 
AATACCCTTC GCTCAAAGTC ACCAATGCAG TGGGACAATA GTTCAAATGC TGGTTTTTCT 
GAAGCTAGTA ACACCTGGTT ACCTACCAAT TCAGATTACC ACACTGTGAA TGTTGATTCT 
TGCTCCGTCA CCCATGCTCG AGTGCAGTGG CGTGATCTCG GCACACTGCA GCCTCTGCTT 
CCTGGGTTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SLC3A1 ( XM_011533047.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011531349.1 Homo sapiens solute carrier family 3 member 1 (SLC3A1), transcript variant X1, mRNA.

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CloneID	OHu60547
Clone ID Related Accession (Same CDS sequence)	XM_011533047.2 , XM_011533047.3
Accession Version	XM_011533047.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1695bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	neutral and basic amino acid transport protein rBAT isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011533047.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGAAG ATAAAAGCAA GAGAGACTCC ATCGAGATGA GTATGAAGGG ATGCCAGACA 
AACAACGGGT TTGTCCATAA TGAAGACATT CTGGAGCAGA CCCCGGATCC AGGAAGCTCA 
ACAGACAACC TGAAGCACAG CACCAGGGGC ATCCTTGGCT CCCAGGAGCC CGACTTCAAG 
GGCGTCCAGC CCTATGCGGG GATGCCCAAG GAGGTGCTGT TCCAGTTCTC TGGCCAGGCC 
CGCTACCGCA TACCTCGGGA GATCCTCTTC TGGCTCACAG TGGCTTCTGT GCTGGTGCTC 
ATCGCGGCCA CCATAGCCAT CATTGCCCTC TCTCCAAAGT GCCTAGACTG GTGGCAGGAG 
GGGCCCATGT ACCAGATCTA CCCAAGGTCT TTCAAGGACA GTAACAAGGA TGGGAACGGA 
GATCTGAAAG GTATTCAAGA TAAACTGGAC TACATCACAG CTTTAAATAT AAAAACTGTT 
TGGATTACTT CATTTTATAA ATCGTCCCTT AAAGATTTCA GATATGGTGT TGAAGATTTC 
CGGGAAGTTG ATCCCATTTT TGGAACGATG GAAGATTTTG AGAATCTGGT TGCAGCCATA 
CATGATAAAG GTTTAAAATT AATCATCGAT TTCATACCAA ACCACACGAG TGATAAACAT 
ATTTGGTTTC AATTGAGTCG GACACGGACA GGAAAATATA CTGATTATTA TATCTGGCAT 
GACTGTACCC ATGAAAATGG CAAAACCATT CCACCCAACA ACTGGTTAAG TGTGTATGGA 
AACTCCAGTT GGCACTTTGA CGAAGTGCGA AACCAATGTT ATTTTCATCA GTTTATGAAA 
GAGCAACCTG ATTTAAATTT CCGCAATCCT GATGTTCAAG AAGAAATAAA AGAAATTTTA 
CGGTTCTGGC TCACAAAGGG TGTTGATGGT TTTAGTTTGG ATGCTGTTAA ATTCCTCCTA 
GAAGCAAAGC ACCTGAGAGA TGAGATCCAA GTAAATAAGA CCCAAATCCC GGACACGGTC 
ACACAATACT CGGAGCTGTA CCATGACTTC ACCACCACGC AGGTGGGAAT GCACGACATT 
GTCCGCAGCT TCCGGCAGAC CATGGACCAA TACAGCACGG AGCCCGGCAG ATACAGGTTC 
ATGGGGACTG AAGCCTATGC AGAGAGTATT GACAGGACCG TGATGTACTA TGGATTGCCA 
TTTATCCAAG AAGCTGATTT TCCCTTCAAC AATTACCTCA GCATGCTAGA CACTGTTTCT 
GGGAACAGCG TGTATGAGGT TATCACATCC TGGATGGAAA ACATGCCAGA AGGAAAATGG 
CCTAACTGGA TGATTGGTGG ACCAGACAGT TCACGGCTGA CTTCGCGTTT GGGGAATCAG 
TATGTCAACG TGATGAACAT GCTTCTTTTC ACACTCCCTG GAACTCCTAT AACTTACTAT 
GGAGAAGAAA TTGGAATGGG AAATATTGTA GCCGCAAATC TCAATGAAAG CTATGATATT 
AATACCCTTC GCTCAAAGTC ACCAATGCAG TGGGACAATA GTTCAAATGC TGGTTTTTCT 
GAAGCTAGTA ACACCTGGTT ACCTACCAAT TCAGATTACC ACACTGTGAA TGTTGATTCT 
TGCTCCGTCA CCCATGCTCG AGTGCAGTGG CGTGATCTCG GCACACTGCA GCCTCTGCTT 
CCTGGGTTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011531349.1
CDS	73..1767
Translation	MAEDKSKRDSIEMSMKGCQTNNGFVHNEDILEQTPDPGSSTDNLKHSTRGILGSQEPDFKGVQPYAGMPKEVLFQFSGQARYRIPREILFWLTVASVLVLIAATIAIIALSPKCLDWWQEGPMYQIYPRSFKDSNKDGNGDLKGIQDKLDYITALNIKTVWITSFYKSSLKDFRYGVEDFREVDPIFGTMEDFENLVAAIHDKGLKLIIDFIPNHTSDKHIWFQLSRTRTGKYTDYYIWHDCTHENGKTIPPNNWLSVYGNSSWHFDEVRNQCYFHQFMKEQPDLNFRNPDVQEEIKEILRFWLTKGVDGFSLDAVKFLLEAKHLRDEIQVNKTQIPDTVTQYSELYHDFTTTQVGMHDIVRSFRQTMDQYSTEPGRYRFMGTEAYAESIDRTVMYYGLPFIQEADFPFNNYLSMLDTVSGNSVYEVITSWMENMPEGKWPNWMIGGPDSSRLTSRLGNQYVNVMNMLLFTLPGTPITYYGEEIGMGNIVAANLNESYDINTLRSKSPMQWDNSSNAGFSEASNTWLPTNSDYHTVNVDSCSVTHARVQWRDLGTLQPLLPGFK

Target ORF information:

RefSeq Version	XM_011533047.3
Organism	Homo sapiens(human)
Definition	Homo sapiens solute carrier family 3 member 1 (SLC3A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533047.3

ORF Insert Sequence:

ATGGCTGAAG ATAAAAGCAA GAGAGACTCC ATCGAGATGA GTATGAAGGG ATGCCAGACA 
AACAACGGGT TTGTCCATAA TGAAGACATT CTGGAGCAGA CCCCGGATCC AGGAAGCTCA 
ACAGACAACC TGAAGCACAG CACCAGGGGC ATCCTTGGCT CCCAGGAGCC CGACTTCAAG 
GGCGTCCAGC CCTATGCGGG GATGCCCAAG GAGGTGCTGT TCCAGTTCTC TGGCCAGGCC 
CGCTACCGCA TACCTCGGGA GATCCTCTTC TGGCTCACAG TGGCTTCTGT GCTGGTGCTC 
ATCGCGGCCA CCATAGCCAT CATTGCCCTC TCTCCAAAGT GCCTAGACTG GTGGCAGGAG 
GGGCCCATGT ACCAGATCTA CCCAAGGTCT TTCAAGGACA GTAACAAGGA TGGGAACGGA 
GATCTGAAAG GTATTCAAGA TAAACTGGAC TACATCACAG CTTTAAATAT AAAAACTGTT 
TGGATTACTT CATTTTATAA ATCGTCCCTT AAAGATTTCA GATATGGTGT TGAAGATTTC 
CGGGAAGTTG ATCCCATTTT TGGAACGATG GAAGATTTTG AGAATCTGGT TGCAGCCATA 
CATGATAAAG GTTTAAAATT AATCATCGAT TTCATACCAA ACCACACGAG TGATAAACAT 
ATTTGGTTTC AATTGAGTCG GACACGGACA GGAAAATATA CTGATTATTA TATCTGGCAT 
GACTGTACCC ATGAAAATGG CAAAACCATT CCACCCAACA ACTGGTTAAG TGTGTATGGA 
AACTCCAGTT GGCACTTTGA CGAAGTGCGA AACCAATGTT ATTTTCATCA GTTTATGAAA 
GAGCAACCTG ATTTAAATTT CCGCAATCCT GATGTTCAAG AAGAAATAAA AGAAATTTTA 
CGGTTCTGGC TCACAAAGGG TGTTGATGGT TTTAGTTTGG ATGCTGTTAA ATTCCTCCTA 
GAAGCAAAGC ACCTGAGAGA TGAGATCCAA GTAAATAAGA CCCAAATCCC GGACACGGTC 
ACACAATACT CGGAGCTGTA CCATGACTTC ACCACCACGC AGGTGGGAAT GCACGACATT 
GTCCGCAGCT TCCGGCAGAC CATGGACCAA TACAGCACGG AGCCCGGCAG ATACAGGTTC 
ATGGGGACTG AAGCCTATGC AGAGAGTATT GACAGGACCG TGATGTACTA TGGATTGCCA 
TTTATCCAAG AAGCTGATTT TCCCTTCAAC AATTACCTCA GCATGCTAGA CACTGTTTCT 
GGGAACAGCG TGTATGAGGT TATCACATCC TGGATGGAAA ACATGCCAGA AGGAAAATGG 
CCTAACTGGA TGATTGGTGG ACCAGACAGT TCACGGCTGA CTTCGCGTTT GGGGAATCAG 
TATGTCAACG TGATGAACAT GCTTCTTTTC ACACTCCCTG GAACTCCTAT AACTTACTAT 
GGAGAAGAAA TTGGAATGGG AAATATTGTA GCCGCAAATC TCAATGAAAG CTATGATATT 
AATACCCTTC GCTCAAAGTC ACCAATGCAG TGGGACAATA GTTCAAATGC TGGTTTTTCT 
GAAGCTAGTA ACACCTGGTT ACCTACCAAT TCAGATTACC ACACTGTGAA TGTTGATTCT 
TGCTCCGTCA CCCATGCTCG AGTGCAGTGG CGTGATCTCG GCACACTGCA GCCTCTGCTT 
CCTGGGTTCA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Clinical and genetic characterization of Chinese pediatric cystine stone patients.
Journal of pediatric urology13(6)629.e1-629.e5(2017 Dec)
Shen L,Cong X,Zhang X,Wang N,Zhou P,Xu Y,Zhu Q,Gu X

Spectrum of mutations in cystinuria patients presenting with prenatal hyperechoic colon.
Clinical genetics92(6)632-638(2017 Dec)
Tostivint I,Royer N,Nicolas M,Bourillon A,Czerkiewicz I,Becker PH,Muller F,Benoist JF

Associating mutations causing cystinuria with disease severity with the aim of providing precision medicine.
BMC genomics18(Suppl 5)550(2017 Aug)
Martell HJ,Wong KA,Martin JF,Kassam Z,Thomas K,Wass MN

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SLC3A1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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