SIX3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SIX3
Entrez Gene ID	6496
Full Name	SIX homeobox 3
Synonyms	HPE2
General protein information	Preferred Names SIX homeobox 3 Names homeobox protein SIX3 sine oculis homeobox homolog 3 sine oculis homeobox-like protein 3 truncated SIX3
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 2 Map Location: 2p21
Summary	This gene encodes a member of the sine oculis homeobox transcription factor family. The encoded protein plays a role in eye development. Mutations in this gene have been associated with holoprosencephaly type 2. [provided by RefSeq, Oct 2009].
Disorder MIM:	603714
Disorder Html:	Holoprosencephaly 2, 157170 (3); Schizencephaly, 269160 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_005413.3	NP_005404.1	homeobox protein SIX3
NM_005413.4	NP_005404.1	homeobox protein SIX3

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	six3	XP_002944388.2
Canis lupus familiaris (dog)	SIX3	XP_538477.4
Mus musculus (house mouse)	Six3	NP_035511.2
Rattus norvegicus (Norway rat)	Six3	NP_076480.1
Danio rerio (zebrafish)	six3a	NP_571437.1
Pan troglodytes (chimpanzee)	SIX3	XP_525749.4
Bos taurus (cattle)	SIX3	NP_001180053.1
Gallus gallus (chicken)	SIX3	NP_989695.1
Homo sapiens (human)	SIX3	NP_005404.1

Gene Ontology
Bibliography

Related articles in PubMed

SIX3 deletions and incomplete penetrance in families affected by holoprosencephaly.
Stokes B, Berger SI, Hall BA, Weiss K, Martinez AF, Hadley DW, Murdock DR, Ramanathan S, Clark RD, Roessler E, Kruszka P, Muenke M
Congenital anomalies58(1)29-32(2018 Jan)

Epigenetically controlled Six3 expression regulates glioblastoma cell proliferation and invasion alongside modulating the activation levels of WNT pathway members.
Zhang B, Shen C, Ge F, Ma T, Zhang Z
Journal of neuro-oncology133(3)509-518(2017 Jul)

The expression profile and clinic significance of the SIX family in non-small cell lung cancer.
Liu Q, Li A, Tian Y, Liu Y, Li T, Zhang C, Wu JD, Han X, Wu K
Journal of hematology & oncology9(1)119(2016 11)

Genomic Alteration in Head and Neck Squamous Cell Carcinoma (HNSCC) Cell Lines Inferred from Karyotyping, Molecular Cytogenetics, and Array Comparative Genomic Hybridization.
Singchat W, Hitakomate E, Rerkarmnuaychoke B, Suntronpong A, Fu B, Bodhisuwan W, Peyachoknagul S, Yang F, Koontongkaew S, Srikulnath K
PloS one11(8)e0160901(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

SIX3 is a novel negative transcriptional regulator and acts as a tumor suppressor that directly represses the transcription of AURKA and AURKB in astrocytoma.
Title: SIX3, a tumor suppressor, inhibits astrocytoma tumorigenesis by transcriptional repression of AURKA/B.

SIX2, SIX3, and SIX4 were correlated with higher TNM stages in lung neoplasms
Title: The expression profile and clinic significance of the SIX family in non-small cell lung cancer.

High SIX3 expression is associated with Head and Neck Squamous Cell Carcinoma.
Title: Genomic Alteration in Head and Neck Squamous Cell Carcinoma (HNSCC) Cell Lines Inferred from Karyotyping, Molecular Cytogenetics, and Array Comparative Genomic Hybridization.

SIX3 may play an important role as a novel suppressor in human lung cancer.
Title: Down-regulation of SIX3 is associated with clinical outcome in lung adenocarcinoma.

Mutations in SIX3 is associated with holoprosencephaly.
Title: Utilizing prospective sequence analysis of SHH, ZIC2, SIX3 and TGIF in holoprosencephaly probands to describe the parameters limiting the observed frequency of mutant gene?gene interactions.

The following SIX3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIX3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22691	NM_005413.4 Latest version!	Homo sapiens SIX homeobox 3 (SIX3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
View Old Accession Versions >>
OHu22691	NM_005413.3	Homo sapiens SIX homeobox 3 (SIX3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$154.50-$216.30 $309.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIX3 ( NM_005413.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_005404.1 Homo sapiens SIX homeobox 3 (SIX3), mRNA.

Price & Availability↑

CloneID	OHu22691
Clone ID Related Accession (Same CDS sequence)	NM_005413.3 , NM_005413.4
Accession Version	NM_005413.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-05
Organism	Homo sapiens(human)
Product	homeobox protein SIX3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ012611.1, BF570321.1, EL952320.1, AC012354.11, CD673488.1 and BM668514.1. This sequence is a reference standard in the RefSeqGene project. On Oct 23, 2009 this sequence version replaced NM_005413.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ012611.1, BF570321.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTATTCC GCTCCCCCCT AGACCTCTAT TCCTCCCACT TCTTGTTGCC AAACTTCGCC 
GATTCTCACC ACCGCTCCAT ACTTCTGGCG AGTAGCGGCG GCGGGAACGG TGCGGGAGGC 
GGCGGCGGCG CGGGAGGCGG CAGCGGCGGC GGGAACGGTG CGGGAGGCGG CGGTGCTGGC 
GGAGCAGGCG GCGGCGGCGG CGGCGGCTCC AGGGCCCCCC CGGAAGAGTT GTCCATGTTC 
CAGCTGCCCA CCCTCAACTT CTCGCCGGAG CAGGTGGCCA GCGTCTGTGA GACGCTGGAG 
GAGACGGGCG ACATCGAGCG GCTGGGCCGC TTCCTCTGGT CGCTGCCCGT GGCCCCCGGG 
GCGTGCGAGG CCATCAACAA ACACGAGTCG ATCCTGCGCG CGCGCGCCGT GGTCGCCTTC 
CACACGGGCA ACTTCCGCGA CCTCTACCAC ATCCTTGAGA ACCACAAGTT CACCAAGGAG 
TCTCACGGCA AGCTGCAGGC CATGTGGCTC GAGGCGCACT ACCAGGAGGC CGAGAAGCTG 
CGCGGCCGCC CACTCGGCCC GGTGGACAAG TACCGCGTGC GCAAGAAGTT CCCGCTGCCA 
CGCACCATCT GGGACGGCGA GCAGAAGACG CATTGCTTCA AGGAGCGGAC TCGGAGCCTG 
TTGCGGGAGT GGTACCTACA GGACCCCTAC CCCAACCCCA GCAAGAAACG CGAACTGGCG 
CAGGCCACCG GCCTCACTCC CACACAAGTA GGCAACTGGT TTAAGAACCG GCGGCAGCGC 
GACCGCGCCG CGGCGGCCAA GAACAGGCTC CAGCACCAGG CCATTGGACC GAGCGGCATG 
CGCTCGCTGG CCGAGCCCGG CTGCCCCACG CACGGCTCGG CAGAGTCGCC GTCCACGGCG 
GCCAGCCCGA CCACCAGCGT GTCCAGCCTG ACGGAGCGCG CAGACACCGG CACCTCCATC 
CTCTCGGTAA CCTCCAGCGA CTCGGAATGT GATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005404.1
CDS	208..1206
Translation	MVFRSPLDLYSSHFLLPNFADSHHRSILLASSGGGNGAGGGGGAGGGSGGGNGAGGGGAGGAGGGGGGGSRAPPEELSMFQLPTLNFSPEQVASVCETLEETGDIERLGRFLWSLPVAPGACEAINKHESILRARAVVAFHTGNFRDLYHILENHKFTKESHGKLQAMWLEAHYQEAEKLRGRPLGPVDKYRVRKKFPLPRTIWDGEQKTHCFKERTRSLLREWYLQDPYPNPSKKRELAQATGLTPTQVGNWFKNRRQRDRAAAAKNRLQHQAIGPSGMRSLAEPGCPTHGSAESPSTAASPTTSVSSLTERADTGTSILSVTSSDSECDV

Target ORF information:

RefSeq Version	NM_005413.3
Organism	Homo sapiens(human)
Definition	Homo sapiens SIX homeobox 3 (SIX3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005413.3

ORF Insert Sequence:

ATGGTATTCC GCTCCCCCCT AGACCTCTAT TCCTCCCACT TCTTGTTGCC AAACTTCGCC 
GATTCTCACC ACCGCTCCAT ACTTCTGGCG AGTAGCGGCG GCGGGAACGG TGCGGGAGGC 
GGCGGCGGCG CGGGAGGCGG CAGCGGCGGC GGGAACGGTG CGGGAGGCGG CGGTGCTGGC 
GGAGCAGGCG GCGGCGGCGG CGGCGGCTCC AGGGCCCCCC CGGAAGAGTT GTCCATGTTC 
CAGCTGCCCA CCCTCAACTT CTCGCCGGAG CAGGTGGCCA GCGTCTGTGA GACGCTGGAG 
GAGACGGGCG ACATCGAGCG GCTGGGCCGC TTCCTCTGGT CGCTGCCCGT GGCCCCCGGG 
GCGTGCGAGG CCATCAACAA ACACGAGTCG ATCCTGCGCG CGCGCGCCGT GGTCGCCTTC 
CACACGGGCA ACTTCCGCGA CCTCTACCAC ATCCTTGAGA ACCACAAGTT CACCAAGGAG 
TCTCACGGCA AGCTGCAGGC CATGTGGCTC GAGGCGCACT ACCAGGAGGC CGAGAAGCTG 
CGCGGCCGCC CACTCGGCCC GGTGGACAAG TACCGCGTGC GCAAGAAGTT CCCGCTGCCA 
CGCACCATCT GGGACGGCGA GCAGAAGACG CATTGCTTCA AGGAGCGGAC TCGGAGCCTG 
TTGCGGGAGT GGTACCTACA GGACCCCTAC CCCAACCCCA GCAAGAAACG CGAACTGGCG 
CAGGCCACCG GCCTCACTCC CACACAAGTA GGCAACTGGT TTAAGAACCG GCGGCAGCGC 
GACCGCGCCG CGGCGGCCAA GAACAGGCTC CAGCACCAGG CCATTGGACC GAGCGGCATG 
CGCTCGCTGG CCGAGCCCGG CTGCCCCACG CACGGCTCGG CAGAGTCGCC GTCCACGGCG 
GCCAGCCCGA CCACCAGCGT GTCCAGCCTG ACGGAGCGCG CAGACACCGG CACCTCCATC 
CTCTCGGTAA CCTCCAGCGA CTCGGAATGT GATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIX3 ( NM_005413.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005404.1 Homo sapiens SIX homeobox 3 (SIX3), mRNA.

Price & Availability↑

CloneID	OHu22691
Clone ID Related Accession (Same CDS sequence)	NM_005413.3 , NM_005413.4
Accession Version	NM_005413.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 999bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	homeobox protein SIX3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012354.11, AJ012611.1, BF570321.1, EL952320.1 and CD673488.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_005413.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ012611.1, BF570321.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2148874, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000260653.5/ ENSP00000260653.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGTATTCC GCTCCCCCCT AGACCTCTAT TCCTCCCACT TCTTGTTGCC AAACTTCGCC 
GATTCTCACC ACCGCTCCAT ACTTCTGGCG AGTAGCGGCG GCGGGAACGG TGCGGGAGGC 
GGCGGCGGCG CGGGAGGCGG CAGCGGCGGC GGGAACGGTG CGGGAGGCGG CGGTGCTGGC 
GGAGCAGGCG GCGGCGGCGG CGGCGGCTCC AGGGCCCCCC CGGAAGAGTT GTCCATGTTC 
CAGCTGCCCA CCCTCAACTT CTCGCCGGAG CAGGTGGCCA GCGTCTGTGA GACGCTGGAG 
GAGACGGGCG ACATCGAGCG GCTGGGCCGC TTCCTCTGGT CGCTGCCCGT GGCCCCCGGG 
GCGTGCGAGG CCATCAACAA ACACGAGTCG ATCCTGCGCG CGCGCGCCGT GGTCGCCTTC 
CACACGGGCA ACTTCCGCGA CCTCTACCAC ATCCTTGAGA ACCACAAGTT CACCAAGGAG 
TCTCACGGCA AGCTGCAGGC CATGTGGCTC GAGGCGCACT ACCAGGAGGC CGAGAAGCTG 
CGCGGCCGCC CACTCGGCCC GGTGGACAAG TACCGCGTGC GCAAGAAGTT CCCGCTGCCA 
CGCACCATCT GGGACGGCGA GCAGAAGACG CATTGCTTCA AGGAGCGGAC TCGGAGCCTG 
TTGCGGGAGT GGTACCTACA GGACCCCTAC CCCAACCCCA GCAAGAAACG CGAACTGGCG 
CAGGCCACCG GCCTCACTCC CACACAAGTA GGCAACTGGT TTAAGAACCG GCGGCAGCGC 
GACCGCGCCG CGGCGGCCAA GAACAGGCTC CAGCACCAGG CCATTGGACC GAGCGGCATG 
CGCTCGCTGG CCGAGCCCGG CTGCCCCACG CACGGCTCGG CAGAGTCGCC GTCCACGGCG 
GCCAGCCCGA CCACCAGCGT GTCCAGCCTG ACGGAGCGCG CAGACACCGG CACCTCCATC 
CTCTCGGTAA CCTCCAGCGA CTCGGAATGT GATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005404.1
CDS	404..1402
Translation	MVFRSPLDLYSSHFLLPNFADSHHRSILLASSGGGNGAGGGGGAGGGSGGGNGAGGGGAGGAGGGGGGGSRAPPEELSMFQLPTLNFSPEQVASVCETLEETGDIERLGRFLWSLPVAPGACEAINKHESILRARAVVAFHTGNFRDLYHILENHKFTKESHGKLQAMWLEAHYQEAEKLRGRPLGPVDKYRVRKKFPLPRTIWDGEQKTHCFKERTRSLLREWYLQDPYPNPSKKRELAQATGLTPTQVGNWFKNRRQRDRAAAAKNRLQHQAIGPSGMRSLAEPGCPTHGSAESPSTAASPTTSVSSLTERADTGTSILSVTSSDSECDV

Target ORF information:

RefSeq Version	NM_005413.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SIX homeobox 3 (SIX3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005413.4

ORF Insert Sequence:

ATGGTATTCC GCTCCCCCCT AGACCTCTAT TCCTCCCACT TCTTGTTGCC AAACTTCGCC 
GATTCTCACC ACCGCTCCAT ACTTCTGGCG AGTAGCGGCG GCGGGAACGG TGCGGGAGGC 
GGCGGCGGCG CGGGAGGCGG CAGCGGCGGC GGGAACGGTG CGGGAGGCGG CGGTGCTGGC 
GGAGCAGGCG GCGGCGGCGG CGGCGGCTCC AGGGCCCCCC CGGAAGAGTT GTCCATGTTC 
CAGCTGCCCA CCCTCAACTT CTCGCCGGAG CAGGTGGCCA GCGTCTGTGA GACGCTGGAG 
GAGACGGGCG ACATCGAGCG GCTGGGCCGC TTCCTCTGGT CGCTGCCCGT GGCCCCCGGG 
GCGTGCGAGG CCATCAACAA ACACGAGTCG ATCCTGCGCG CGCGCGCCGT GGTCGCCTTC 
CACACGGGCA ACTTCCGCGA CCTCTACCAC ATCCTTGAGA ACCACAAGTT CACCAAGGAG 
TCTCACGGCA AGCTGCAGGC CATGTGGCTC GAGGCGCACT ACCAGGAGGC CGAGAAGCTG 
CGCGGCCGCC CACTCGGCCC GGTGGACAAG TACCGCGTGC GCAAGAAGTT CCCGCTGCCA 
CGCACCATCT GGGACGGCGA GCAGAAGACG CATTGCTTCA AGGAGCGGAC TCGGAGCCTG 
TTGCGGGAGT GGTACCTACA GGACCCCTAC CCCAACCCCA GCAAGAAACG CGAACTGGCG 
CAGGCCACCG GCCTCACTCC CACACAAGTA GGCAACTGGT TTAAGAACCG GCGGCAGCGC 
GACCGCGCCG CGGCGGCCAA GAACAGGCTC CAGCACCAGG CCATTGGACC GAGCGGCATG 
CGCTCGCTGG CCGAGCCCGG CTGCCCCACG CACGGCTCGG CAGAGTCGCC GTCCACGGCG 
GCCAGCCCGA CCACCAGCGT GTCCAGCCTG ACGGAGCGCG CAGACACCGG CACCTCCATC 
CTCTCGGTAA CCTCCAGCGA CTCGGAATGT GATGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

SIX3 deletions and incomplete penetrance in families affected by holoprosencephaly.
Congenital anomalies58(1)29-32(2018 Jan)
Stokes B,Berger SI,Hall BA,Weiss K,Martinez AF,Hadley DW,Murdock DR,Ramanathan S,Clark RD,Roessler E,Kruszka P,Muenke M

Epigenetically controlled Six3 expression regulates glioblastoma cell proliferation and invasion alongside modulating the activation levels of WNT pathway members.
Journal of neuro-oncology133(3)509-518(2017 Jul)
Zhang B,Shen C,Ge F,Ma T,Zhang Z

The expression profile and clinic significance of the SIX family in non-small cell lung cancer.
Journal of hematology & oncology9(1)119(2016 11)
Liu Q,Li A,Tian Y,Liu Y,Li T,Zhang C,Wu JD,Han X,Wu K

Genomic Alteration in Head and Neck Squamous Cell Carcinoma (HNSCC) Cell Lines Inferred from Karyotyping, Molecular Cytogenetics, and Array Comparative Genomic Hybridization.
PloS one11(8)e0160901(2016)
Singchat W,Hitakomate E,Rerkarmnuaychoke B,Suntronpong A,Fu B,Bodhisuwan W,Peyachoknagul S,Yang F,Koontongkaew S,Srikulnath K