ITSN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ITSN1
Entrez Gene ID	6453
Full Name	intersectin 1
Synonyms	ITSN,SH3D1A,SH3P17
General protein information	Preferred Names intersectin 1 Names intersectin-1 SH3 domain-containing protein 1A Src homology 3 domain-containing protein human intersectin-SH3 domain-containing protein SH3P17 intersectin 1 (SH3 domain protein) intersectin 1 short form variant 3 intersectin 1 short form variant, 11 intersectin short variant 12
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 21 Map Location: 21q22.11
Summary	The protein encoded by this gene is a cytoplasmic membrane-associated protein that indirectly coordinates endocytic membrane traffic with the actin assembly machinery. In addition, the encoded protein may regulate the formation of clathrin-coated vesicles and could be involved in synaptic vesicle recycling. This protein has been shown to interact with dynamin, CDC42, SNAP23, SNAP25, SPIN90, EPS15, EPN1, EPN2, and STN2. Multiple transcript variants encoding different isoforms have been found for this gene, but the full-length nature of only two of them have been characterized so far. [provided by RefSeq, Jul 2008].
Disorder MIM:	602442

mRNA and Protein(s)

mRNA	Protein	Name
XM_017028432.1	XP_016883921.1	intersectin-1 isoform X6
NM_001001132.1	NP_001001132.1	intersectin-1 isoform ITSN-s
XM_017028438.1	XP_016883927.1	intersectin-1 isoform X13
XM_017028440.1	XP_016883929.1	intersectin-1 isoform X17
NM_001331008.1	NP_001317937.1	intersectin-1 isoform 3
NM_001331010.1	NP_001317939.1	intersectin-1 isoform 5
XM_017028430.2	XP_016883919.1	intersectin-1 isoform X3
XM_017028431.2	XP_016883920.1	intersectin-1 isoform X4
XM_017028440.2	XP_016883929.1	intersectin-1 isoform X12
NM_003024.3	NP_003015.2	intersectin-1 isoform ITSN-l
NM_001001132.2	NP_001001132.1	intersectin-1 isoform ITSN-s
XM_017028434.1	XP_016883923.1	intersectin-1 isoform X8
XM_017028430.1	XP_016883919.1	intersectin-1 isoform X4
XM_017028429.1	XP_016883918.1	intersectin-1 isoform X3
XM_011529693.2	XP_011527995.1	intersectin-1 isoform X20
NM_001331008.2	NP_001317937.1	intersectin-1 isoform 3
XM_017028428.1	XP_016883917.1	intersectin-1 isoform X1
XM_017028432.2	XP_016883921.1	intersectin-1 isoform X5
NM_001331009.2	NP_001317938.1	intersectin-1 isoform 4
NM_003024.2	NP_003015.2	intersectin-1 isoform ITSN-l
NM_001331011.1	NP_001317940.1	intersectin-1 isoform 6
NM_001331009.1	NP_001317938.1	intersectin-1 isoform 4
XM_017028436.2	XP_016883925.1	intersectin-1 isoform X9
XM_017028437.1	XP_016883926.1	intersectin-1 isoform X10
XM_017028438.2	XP_016883927.1	intersectin-1 isoform X11
XM_017028441.2	XP_016883930.1	intersectin-1 isoform X14
XM_011529693.3	XP_011527995.1	intersectin-1 isoform X15
NM_001331010.2	NP_001317939.1	intersectin-1 isoform 5
XM_011529692.1	XP_011527994.1	intersectin-1 isoform X18
XM_017028441.1	XP_016883930.1	intersectin-1 isoform X19
XM_017028436.1	XP_016883925.1	intersectin-1 isoform X10
XM_017028431.1	XP_016883920.1	intersectin-1 isoform X5
XM_017028433.1	XP_016883922.1	intersectin-1 isoform X7
XM_017028435.1	XP_016883924.1	intersectin-1 isoform X9
NM_001331012.1	NP_001317941.1	intersectin-1 isoform 7
NM_001331011.2	NP_001317940.1	intersectin-1 isoform 6
NM_001331012.2	NP_001317941.1	intersectin-1 isoform 7
XM_017028429.2	XP_016883918.1	intersectin-1 isoform X2
XM_017028433.2	XP_016883922.1	intersectin-1 isoform X6
XM_017028434.2	XP_016883923.1	intersectin-1 isoform X7
XM_017028435.2	XP_016883924.1	intersectin-1 isoform X8
XM_011529692.2	XP_011527994.1	intersectin-1 isoform X13

Pathways from BioSystems

Homologies

Homo sapiens (human)	ITSN1	NP_003015.2
Canis lupus familiaris (dog)	ITSN1	XP_005638937.1
Drosophila melanogaster (fruit fly)	Dap160	NP_001246118.1
Xenopus tropicalis (tropical clawed frog)	itsn1	XP_002939390.1
Bos taurus (cattle)	ITSN1	XP_002707731.1
Mus musculus (house mouse)	Itsn1	NP_034717.2
Gallus gallus (chicken)	ITSN1	XP_416715.2
	ITSN1	XP_002803192.1
Rattus norvegicus (Norway rat)	Itsn1	NP_001129568.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009037	XP_319789.4
Pan troglodytes (chimpanzee)	ITSN1	XP_001166935.1
Canis lupus familiaris (dog)	LOC100856635	XP_003640127.1
Danio rerio (zebrafish)	itsn1	NP_997065.1
Caenorhabditis elegans (roundworm)	itsn-1	NP_001255927.1

Gene Ontology
Bibliography

Related articles in PubMed

Intersectin-s interaction with DENND2B facilitates recycling of epidermal growth factor receptor.
Ioannou MS, Kulasekaran G, Fotouhi M, Morein JJ, Han C, Tse S, Nossova N, Han T, Mannard E, McPherson PS
EMBO reports18(12)2119-2130(2017 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Silencing ITSN1 in neuroblastoma cells led to decreased tumor growth in an orthotopic mouse model. Orthotopic animal models can provide insight into the role of ITSN1 pathways in neuroblastoma tumorigenesis.
Title: Silencing Intersectin 1 Slows Orthotopic Neuroblastoma Growth in Mice.

These findings provide the basis for further functional investigations of the ITSN/CR16 complex that may play an important role in actin remodeling and cellular invasion.
Title: Mammalian verprolin CR16 acts as a modulator of ITSN scaffold proteins association with actin.

Study suggests critical roles of ITSN1-S in malignant glioma proliferation, indicating a potential usage of ITSN1-S in the therapeutic intervention as a novel molecular target
Title: Intersectin1-S, a multidomain adapter protein, is essential for malignant glioma proliferation.

Abnormal expression of the intersectin1-L protein in epileptic brain tissue may play an important role in epilepsy, especially refractory epilepsy.
Title: Altered Expression of Intersectin1-L in Patients with Refractory Epilepsy and in Experimental Epileptic Rats.

Our observations suggest that ITSN1 is an important general regulator of Cdc42-, Nck- and N-WASP-dependent actin polymerisation
Title: Cdc42 and the Rho GEF intersectin-1 collaborate with Nck to promote N-WASP-dependent actin polymerisation.

The following ITSN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ITSN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu102174	NM_001331008.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu93301	XM_017028437.1 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu93302	XM_017028438.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu93303	XM_017028440.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu93304	XM_017028441.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu10132	NM_001001132.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$860.30 $1229.00
OHu53297	NM_001331009.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu53298	NM_001331011.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu60474	NM_001331012.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu60475	XM_011529692.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu60476	XM_011529693.3 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu93293	XM_017028429.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu93294	XM_017028430.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu93295	XM_017028431.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu93296	XM_017028432.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu93297	XM_017028433.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu93298	XM_017028434.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu93299	XM_017028435.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu93300	XM_017028436.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu17805	NM_003024.3 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu53299	NM_001331010.2 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu17805	XM_017028428.1 Latest version!	Homo sapiens intersectin 1 (ITSN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
View Old Accession Versions >>
OHu93303	XM_017028440.1	Homo sapiens intersectin 1 (ITSN1), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu10132	NM_001001132.1	Homo sapiens intersectin 1 (ITSN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$860.30 $1229.00
OHu93294	XM_017028430.1	Homo sapiens intersectin 1 (ITSN1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu102174	NM_001331008.1	Homo sapiens intersectin 1 (ITSN1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu93296	XM_017028432.1	Homo sapiens intersectin 1 (ITSN1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu17805	NM_003024.2	Homo sapiens intersectin 1 (ITSN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu53297	NM_001331009.1	Homo sapiens intersectin 1 (ITSN1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu93302	XM_017028438.1	Homo sapiens intersectin 1 (ITSN1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OHu60476	XM_011529693.2	Homo sapiens intersectin 1 (ITSN1), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu53299	NM_001331010.1	Homo sapiens intersectin 1 (ITSN1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$951.30 $1359.00
OHu93304	XM_017028441.1	Homo sapiens intersectin 1 (ITSN1), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu93300	XM_017028436.1	Homo sapiens intersectin 1 (ITSN1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu93295	XM_017028431.1	Homo sapiens intersectin 1 (ITSN1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu53298	NM_001331011.1	Homo sapiens intersectin 1 (ITSN1), transcript variant 6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu60474	NM_001331012.1	Homo sapiens intersectin 1 (ITSN1), transcript variant 7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu93293	XM_017028429.1	Homo sapiens intersectin 1 (ITSN1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OHu93297	XM_017028433.1	Homo sapiens intersectin 1 (ITSN1), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu93298	XM_017028434.1	Homo sapiens intersectin 1 (ITSN1), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu93299	XM_017028435.1	Homo sapiens intersectin 1 (ITSN1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu60475	XM_011529692.1	Homo sapiens intersectin 1 (ITSN1), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ITSN1 ( NM_001331008.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317937.1 Homo sapiens intersectin 1 (ITSN1), transcript variant 3, mRNA.

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CloneID	OHu102174
Clone ID Related Accession (Same CDS sequence)	NM_001331008.1 , NM_001331008.2
Accession Version	NM_001331008.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	intersectin-1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK027846.1, AP000308.1, AP000311.1, KF511399.1, AP000312.2 and CX163355.1. On Sep 3, 2016 this sequence version replaced XM_017028439.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.91155.1, DQ679755.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAAAT TGCCCAGGTT ATTGCCTCAT ACACCGCCAC CGGCCCCGAG 
CAGCTCACTC TCGCCCCTGG TCAGCTGATT TTGATCCGAA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTGCAAGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT CCCAGCTAAT 
TATGTAAAGC TTCTAAGCCC TGGGACGAGC AAAATCACTC CAACAGAGCC ACCTAAGTCA 
ACAGCATTAG CGGCAGTGTG CCAGGTGATT GGGATGTACG ACTACACCGC GCAGAATGAC 
GATGAGCTGG CCTTCAACAA GGGCCAGATC ATCAACGTCC TCAACAAGGA GGACCCTGAC 
TGGTGGAAAG GAGAAGTCAA TGGACAAGTG GGGCTCTTCC CATCCAATTA TGTGAAGCTG 
ACCACAGACA TGGACCCAAG CCAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317937.1
CDS	347..3796
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	NM_001331008.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331008.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAAAT TGCCCAGGTT ATTGCCTCAT ACACCGCCAC CGGCCCCGAG 
CAGCTCACTC TCGCCCCTGG TCAGCTGATT TTGATCCGAA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTGCAAGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT CCCAGCTAAT 
TATGTAAAGC TTCTAAGCCC TGGGACGAGC AAAATCACTC CAACAGAGCC ACCTAAGTCA 
ACAGCATTAG CGGCAGTGTG CCAGGTGATT GGGATGTACG ACTACACCGC GCAGAATGAC 
GATGAGCTGG CCTTCAACAA GGGCCAGATC ATCAACGTCC TCAACAAGGA GGACCCTGAC 
TGGTGGAAAG GAGAAGTCAA TGGACAAGTG GGGCTCTTCC CATCCAATTA TGTGAAGCTG 
ACCACAGACA TGGACCCAAG CCAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( NM_001331008.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317937.1 Homo sapiens intersectin 1 (ITSN1), transcript variant 3, mRNA.

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CloneID	OHu102174
Clone ID Related Accession (Same CDS sequence)	NM_001331008.1 , NM_001331008.2
Accession Version	NM_001331008.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3450bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	intersectin-1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK027846.1, AP000308.1, AP000311.1, KF511399.1, AP000312.2 and CX163355.1. On May 31, 2019 this sequence version replaced NM_001331008.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.91155.1, DQ679755.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAAAT TGCCCAGGTT ATTGCCTCAT ACACCGCCAC CGGCCCCGAG 
CAGCTCACTC TCGCCCCTGG TCAGCTGATT TTGATCCGAA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTGCAAGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT CCCAGCTAAT 
TATGTAAAGC TTCTAAGCCC TGGGACGAGC AAAATCACTC CAACAGAGCC ACCTAAGTCA 
ACAGCATTAG CGGCAGTGTG CCAGGTGATT GGGATGTACG ACTACACCGC GCAGAATGAC 
GATGAGCTGG CCTTCAACAA GGGCCAGATC ATCAACGTCC TCAACAAGGA GGACCCTGAC 
TGGTGGAAAG GAGAAGTCAA TGGACAAGTG GGGCTCTTCC CATCCAATTA TGTGAAGCTG 
ACCACAGACA TGGACCCAAG CCAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317937.1
CDS	246..3695
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	NM_001331008.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331008.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAAAT TGCCCAGGTT ATTGCCTCAT ACACCGCCAC CGGCCCCGAG 
CAGCTCACTC TCGCCCCTGG TCAGCTGATT TTGATCCGAA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTGCAAGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT CCCAGCTAAT 
TATGTAAAGC TTCTAAGCCC TGGGACGAGC AAAATCACTC CAACAGAGCC ACCTAAGTCA 
ACAGCATTAG CGGCAGTGTG CCAGGTGATT GGGATGTACG ACTACACCGC GCAGAATGAC 
GATGAGCTGG CCTTCAACAA GGGCCAGATC ATCAACGTCC TCAACAAGGA GGACCCTGAC 
TGGTGGAAAG GAGAAGTCAA TGGACAAGTG GGGCTCTTCC CATCCAATTA TGTGAAGCTG 
ACCACAGACA TGGACCCAAG CCAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028437.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883926.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X10, mRNA.

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CloneID	OHu93301
Clone ID Related Accession (Same CDS sequence)	XM_017028437.1
Accession Version	XM_017028437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3558bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTGGAA GACTTATTCC AGAAAAACAG ATACTCAATG ACCAATTAAA ACAAGTTCAG 
CAGAACAGTT TGCACAGAGA TTCACTTGTT ACACTTAAAA GAGCCTTAGA AGCAAAAGAA 
CTAGCTCGGC AGCACCTACG AGACCAACTG GATGAAGTGG AGAAAGAAAC TAGATCAAAA 
CTACAGGAGA TTGATATTTT CAATAATCAG CTGAAGGAAC TAAGAGAAAT ACACAATAAG 
CAACAACTCC AGAAGCAAAA GTCCATGGAG GCTGAACGAC TGAAACAGAA AGAACAAGAA 
CGAAAGATCA TAGAATTAGA AAAACAAAAA GAAGAAGCCC AAAGACGAGC TCAGGAAAGG 
GACAAGCAGT GGCTGGAGCA TGTGCAGCAG GAGGACGAGC ATCAGAGACC AAGAAAACTC 
CACGAAGAGG AAAAACTGAA AAGGGAGGAG AGTGTCAAAA AGAAGGATGG CGAGGAAAAA 
GGCAAACAGG AAGCACAAGA CAAGCTGGGT CGGCTTTTCC ATCAACACCA AGAACCAGCT 
AAGCCAGCTG TCCAGGCACC CTGGTCCACT GCAGAAAAAG GTCCACTTAC CATTTCTGCA 
CAGGAAAATG TAAAAGTGGT GTATTACCGG GCACTGTACC CCTTTGAATC CAGAAGCCAT 
GATGAAATCA CTATCCAGCC AGGAGACATA GTCATGGTTA AAGGGGAATG GGTGGATGAA 
AGCCAAACTG GAGAACCCGG CTGGCTTGGA GGAGAATTAA AAGGAAAGAC AGGGTGGTTC 
CCTGCAAACT ATGCAGAGAA AATCCCAGAA AATGAGGTTC CCGCTCCAGT GAAACCAGTG 
ACTGATTCAA CATCTGCCCC TGCCCCCAAA CTGGCCTTGC GTGAGACCCC CGCCCCTTTG 
GCAGTAACCT CTTCAGAGCC CTCCACGACC CCTAATAACT GGGCCGACTT CAGCTCCACG 
TGGCCCACCA GCACGAATGA GAAACCAGAA ACGGATAACT GGGATGCATG GGCAGCCCAG 
CCCTCTCTCA CCGTTCCAAG TGCCGGCCAG TTAAGGCAGA GGTCCGCCTT TACTCCAGCC 
ACGGCCACTG GCTCCTCCCC GTCTCCTGTG CTAGGCCAGG GTGAAAAGGT GGAGGGGCTA 
CAAGCTCAAG CCCTATATCC TTGGAGAGCC AAAAAAGACA ACCACTTAAA TTTTAACAAA 
AATGATGTCA TCACCGTCCT GGAACAGCAA GACATGTGGT GGTTTGGAGA AGTTCAAGGT 
CAGAAGGGTT GGTTCCCCAA GTCTTACGTG AAACTCATTT CAGGGCCCAT AAGGAAGTCT 
ACAAGCATGG ATTCTGGTTC TTCAGAGAGT CCTGCTAGTC TAAAGCGAGT AGCCTCTCCA 
GCAGCCAAGC CGGTCGTTTC GGGAGAAGAA TTTATTGCCA TGTACACTTA CGAGAGTTCT 
GAGCAAGGAG ATTTAACCTT TCAGCAAGGG GATGTGATTT TGGTTACCAA GAAAGATGGT 
GACTGGTGGA CAGGAACAGT GGGCGACAAG GCCGGAGTCT TCCCTTCTAA CTATGTGAGG 
CTTAAAGATT CAGAGGGCTC TGGAACTGCT GGGAAAACAG GGAGTTTAGG AAAAAAACCT 
GAAATTGCCC AGGTTATTGC CTCATACACC GCCACCGGCC CCGAGCAGCT CACTCTCGCC 
CCTGGTCAGC TGATTTTGAT CCGAAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTG 
CAAGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTCCCAG CTAATTATGT AAAGCTTCTA 
AGCCCTGGGA CGAGCAAAAT CACTCCAACA GAGCCACCTA AGTCAACAGC ATTAGCGGCA 
GTGTGCCAGG TGATTGGGAT GTACGACTAC ACCGCGCAGA ATGACGATGA GCTGGCCTTC 
AACAAGGGCC AGATCATCAA CGTCCTCAAC AAGGAGGACC CTGACTGGTG GAAAGGAGAA 
GTCAATGGAC AAGTGGGGCT CTTCCCATCC AATTATGTGA AGCTGACCAC AGACATGGAC 
CCAAGCCAGC AATGGTGTTC AGACTTACAT CTCTTGGATA TGTTGACCCC AACTGAAAGA 
AAGCGACAAG GATACATCCA CGAGCTCATT GTCACCGAGG AGAACTATGT GAATGACCTG 
CAGCTGGTCA CAGAGATTTT TCAAAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA 
GAGGTTGCTA TGATTTTTGT GAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTA 
AAAGCGCTGA GAGTCCGCAA GAAGATGTCC GGGGAGAAGA TGCCTGTGAA GATGATTGGA 
GACATCCTGA GCGCACAGCT GCCGCACATG CAGCCCTACA TCCGCTTCTG CAGCCGCCAG 
CTCAACGGGG CTGCCCTGAT CCAGCAGAAG ACGGATGAGG CCCCAGACTT CAAGGAGTTC 
GTCAAAAGAT TGGCAATGGA TCCTCGGTGT AAAGGGATGC CACTCTCTAG TTTTATACTG 
AAGCCTATGC AACGGGTAAC AAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACC 
CCTGAAAACC ACCCGGACCA CAGCCACTTG AAGCACGCCC TGGAGAAGGC GGAAGAGCTC 
TGTTCCCAGG TGAACGAAGG GGTGCGGGAG AAGGAGAACT CTGACCGGCT GGAGTGGATC 
CAGGCCCACG TGCAGTGTGA AGGCCTGTCT GAGCAACTTG TGTTCAATTC AGTGACCAAT 
TGCTTGGGGC CGCGCAAATT TCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTTTT CAACGACTTC CTCCTGCTGA CTCAGATCAC GAAGCCTTTG 
GGGTCTTCTG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAT 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTAAAATTAC CCACCGACCC TTCTGGAGAC 
GAGCCCATCT TCCACATCTC CCACATTGAC CGCGTCTATA CTCTCCGAGC AGAAAGCATA 
AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCTGCTT CTGAACTCTA CATAGAGACT 
GAGAAAAAGA AGCGCGAGAA AGCGTACCTG GTCCGTTCCC AAAGGGCAAC AGGCATTGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATC GAGTTGAAAC CCTGTCGGTC ACATGGAAAG 
AGCAACCCGT ACTGTGAGGT GACCATGGGT TCCCAGTGCC ACATCACCAA GACGATCCAG 
GACACTCTGA ACCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCCGAGA CCTGGAGCAG 
GAAGTCCTCT GCATCACTGT GTTCGAGAGG GACCAGTTCT CACCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGTGTGGC GGACATCAAG AAAGACCAGG GCTCCAAAGG TCCAGTTACG 
AAGTGTCTTC TGCTGCACGA AGTCCCCACG GGAGAGATTG TGGTCCGCTT GGACCTGCAG 
TTGTTTGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883926.1
CDS	1295..4852
Translation	MLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028437.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028437.1

ORF Insert Sequence:

ATGCTTGGAA GACTTATTCC AGAAAAACAG ATACTCAATG ACCAATTAAA ACAAGTTCAG 
CAGAACAGTT TGCACAGAGA TTCACTTGTT ACACTTAAAA GAGCCTTAGA AGCAAAAGAA 
CTAGCTCGGC AGCACCTACG AGACCAACTG GATGAAGTGG AGAAAGAAAC TAGATCAAAA 
CTACAGGAGA TTGATATTTT CAATAATCAG CTGAAGGAAC TAAGAGAAAT ACACAATAAG 
CAACAACTCC AGAAGCAAAA GTCCATGGAG GCTGAACGAC TGAAACAGAA AGAACAAGAA 
CGAAAGATCA TAGAATTAGA AAAACAAAAA GAAGAAGCCC AAAGACGAGC TCAGGAAAGG 
GACAAGCAGT GGCTGGAGCA TGTGCAGCAG GAGGACGAGC ATCAGAGACC AAGAAAACTC 
CACGAAGAGG AAAAACTGAA AAGGGAGGAG AGTGTCAAAA AGAAGGATGG CGAGGAAAAA 
GGCAAACAGG AAGCACAAGA CAAGCTGGGT CGGCTTTTCC ATCAACACCA AGAACCAGCT 
AAGCCAGCTG TCCAGGCACC CTGGTCCACT GCAGAAAAAG GTCCACTTAC CATTTCTGCA 
CAGGAAAATG TAAAAGTGGT GTATTACCGG GCACTGTACC CCTTTGAATC CAGAAGCCAT 
GATGAAATCA CTATCCAGCC AGGAGACATA GTCATGGTTA AAGGGGAATG GGTGGATGAA 
AGCCAAACTG GAGAACCCGG CTGGCTTGGA GGAGAATTAA AAGGAAAGAC AGGGTGGTTC 
CCTGCAAACT ATGCAGAGAA AATCCCAGAA AATGAGGTTC CCGCTCCAGT GAAACCAGTG 
ACTGATTCAA CATCTGCCCC TGCCCCCAAA CTGGCCTTGC GTGAGACCCC CGCCCCTTTG 
GCAGTAACCT CTTCAGAGCC CTCCACGACC CCTAATAACT GGGCCGACTT CAGCTCCACG 
TGGCCCACCA GCACGAATGA GAAACCAGAA ACGGATAACT GGGATGCATG GGCAGCCCAG 
CCCTCTCTCA CCGTTCCAAG TGCCGGCCAG TTAAGGCAGA GGTCCGCCTT TACTCCAGCC 
ACGGCCACTG GCTCCTCCCC GTCTCCTGTG CTAGGCCAGG GTGAAAAGGT GGAGGGGCTA 
CAAGCTCAAG CCCTATATCC TTGGAGAGCC AAAAAAGACA ACCACTTAAA TTTTAACAAA 
AATGATGTCA TCACCGTCCT GGAACAGCAA GACATGTGGT GGTTTGGAGA AGTTCAAGGT 
CAGAAGGGTT GGTTCCCCAA GTCTTACGTG AAACTCATTT CAGGGCCCAT AAGGAAGTCT 
ACAAGCATGG ATTCTGGTTC TTCAGAGAGT CCTGCTAGTC TAAAGCGAGT AGCCTCTCCA 
GCAGCCAAGC CGGTCGTTTC GGGAGAAGAA TTTATTGCCA TGTACACTTA CGAGAGTTCT 
GAGCAAGGAG ATTTAACCTT TCAGCAAGGG GATGTGATTT TGGTTACCAA GAAAGATGGT 
GACTGGTGGA CAGGAACAGT GGGCGACAAG GCCGGAGTCT TCCCTTCTAA CTATGTGAGG 
CTTAAAGATT CAGAGGGCTC TGGAACTGCT GGGAAAACAG GGAGTTTAGG AAAAAAACCT 
GAAATTGCCC AGGTTATTGC CTCATACACC GCCACCGGCC CCGAGCAGCT CACTCTCGCC 
CCTGGTCAGC TGATTTTGAT CCGAAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTG 
CAAGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTCCCAG CTAATTATGT AAAGCTTCTA 
AGCCCTGGGA CGAGCAAAAT CACTCCAACA GAGCCACCTA AGTCAACAGC ATTAGCGGCA 
GTGTGCCAGG TGATTGGGAT GTACGACTAC ACCGCGCAGA ATGACGATGA GCTGGCCTTC 
AACAAGGGCC AGATCATCAA CGTCCTCAAC AAGGAGGACC CTGACTGGTG GAAAGGAGAA 
GTCAATGGAC AAGTGGGGCT CTTCCCATCC AATTATGTGA AGCTGACCAC AGACATGGAC 
CCAAGCCAGC AATGGTGTTC AGACTTACAT CTCTTGGATA TGTTGACCCC AACTGAAAGA 
AAGCGACAAG GATACATCCA CGAGCTCATT GTCACCGAGG AGAACTATGT GAATGACCTG 
CAGCTGGTCA CAGAGATTTT TCAAAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA 
GAGGTTGCTA TGATTTTTGT GAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTA 
AAAGCGCTGA GAGTCCGCAA GAAGATGTCC GGGGAGAAGA TGCCTGTGAA GATGATTGGA 
GACATCCTGA GCGCACAGCT GCCGCACATG CAGCCCTACA TCCGCTTCTG CAGCCGCCAG 
CTCAACGGGG CTGCCCTGAT CCAGCAGAAG ACGGATGAGG CCCCAGACTT CAAGGAGTTC 
GTCAAAAGAT TGGCAATGGA TCCTCGGTGT AAAGGGATGC CACTCTCTAG TTTTATACTG 
AAGCCTATGC AACGGGTAAC AAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACC 
CCTGAAAACC ACCCGGACCA CAGCCACTTG AAGCACGCCC TGGAGAAGGC GGAAGAGCTC 
TGTTCCCAGG TGAACGAAGG GGTGCGGGAG AAGGAGAACT CTGACCGGCT GGAGTGGATC 
CAGGCCCACG TGCAGTGTGA AGGCCTGTCT GAGCAACTTG TGTTCAATTC AGTGACCAAT 
TGCTTGGGGC CGCGCAAATT TCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTTTT CAACGACTTC CTCCTGCTGA CTCAGATCAC GAAGCCTTTG 
GGGTCTTCTG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAT 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTAAAATTAC CCACCGACCC TTCTGGAGAC 
GAGCCCATCT TCCACATCTC CCACATTGAC CGCGTCTATA CTCTCCGAGC AGAAAGCATA 
AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCTGCTT CTGAACTCTA CATAGAGACT 
GAGAAAAAGA AGCGCGAGAA AGCGTACCTG GTCCGTTCCC AAAGGGCAAC AGGCATTGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATC GAGTTGAAAC CCTGTCGGTC ACATGGAAAG 
AGCAACCCGT ACTGTGAGGT GACCATGGGT TCCCAGTGCC ACATCACCAA GACGATCCAG 
GACACTCTGA ACCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCCGAGA CCTGGAGCAG 
GAAGTCCTCT GCATCACTGT GTTCGAGAGG GACCAGTTCT CACCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGTGTGGC GGACATCAAG AAAGACCAGG GCTCCAAAGG TCCAGTTACG 
AAGTGTCTTC TGCTGCACGA AGTCCCCACG GGAGAGATTG TGGTCCGCTT GGACCTGCAG 
TTGTTTGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028438.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883927.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X13, mRNA.

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CloneID	OHu93302
Clone ID Related Accession (Same CDS sequence)	XM_017028438.1 , XM_017028438.2
Accession Version	XM_017028438.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAATTT ATTGCCATGT ACACTTACGA GAGTTCTGAG 
CAAGGAGATT TAACCTTTCA GCAAGGGGAT GTGATTTTGG TTACCAAGAA AGATGGTGAC 
TGGTGGACAG GAACAGTGGG CGACAAGGCC GGAGTCTTCC CTTCTAACTA TGTGAGGCTT 
AAAGATTCAG AGGGCTCTGG AACTGCTGGG AAAACAGGGA GTTTAGGAAA AAAACCTGAA 
ATTGCCCAGG TTATTGCCTC ATACACCGCC ACCGGCCCCG AGCAGCTCAC TCTCGCCCCT 
GGTCAGCTGA TTTTGATCCG AAAAAAGAAC CCAGGTGGAT GGTGGGAAGG AGAGCTGCAA 
GCACGTGGGA AAAAGCGCCA GATAGGCTGG TTCCCAGCTA ATTATGTAAA GCTTCTAAGC 
CCTGGGACGA GCAAAATCAC TCCAACAGAG CCACCTAAGT CAACAGCATT AGCGGCAGTG 
TGCCAGGTGA TTGGGATGTA CGACTACACC GCGCAGAATG ACGATGAGCT GGCCTTCAAC 
AAGGGCCAGA TCATCAACGT CCTCAACAAG GAGGACCCTG ACTGGTGGAA AGGAGAAGTC 
AATGGACAAG TGGGGCTCTT CCCATCCAAT TATGTGAAGC TGACCACAGA CATGGACCCA 
AGCCAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883927.1
CDS	347..3898
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	XM_017028438.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028438.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAATTT ATTGCCATGT ACACTTACGA GAGTTCTGAG 
CAAGGAGATT TAACCTTTCA GCAAGGGGAT GTGATTTTGG TTACCAAGAA AGATGGTGAC 
TGGTGGACAG GAACAGTGGG CGACAAGGCC GGAGTCTTCC CTTCTAACTA TGTGAGGCTT 
AAAGATTCAG AGGGCTCTGG AACTGCTGGG AAAACAGGGA GTTTAGGAAA AAAACCTGAA 
ATTGCCCAGG TTATTGCCTC ATACACCGCC ACCGGCCCCG AGCAGCTCAC TCTCGCCCCT 
GGTCAGCTGA TTTTGATCCG AAAAAAGAAC CCAGGTGGAT GGTGGGAAGG AGAGCTGCAA 
GCACGTGGGA AAAAGCGCCA GATAGGCTGG TTCCCAGCTA ATTATGTAAA GCTTCTAAGC 
CCTGGGACGA GCAAAATCAC TCCAACAGAG CCACCTAAGT CAACAGCATT AGCGGCAGTG 
TGCCAGGTGA TTGGGATGTA CGACTACACC GCGCAGAATG ACGATGAGCT GGCCTTCAAC 
AAGGGCCAGA TCATCAACGT CCTCAACAAG GAGGACCCTG ACTGGTGGAA AGGAGAAGTC 
AATGGACAAG TGGGGCTCTT CCCATCCAAT TATGTGAAGC TGACCACAGA CATGGACCCA 
AGCCAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028438.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883927.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X11, mRNA.

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CloneID	OHu93302
Clone ID Related Accession (Same CDS sequence)	XM_017028438.1 , XM_017028438.2
Accession Version	XM_017028438.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3552bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028438.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAATTT ATTGCCATGT ACACTTACGA GAGTTCTGAG 
CAAGGAGATT TAACCTTTCA GCAAGGGGAT GTGATTTTGG TTACCAAGAA AGATGGTGAC 
TGGTGGACAG GAACAGTGGG CGACAAGGCC GGAGTCTTCC CTTCTAACTA TGTGAGGCTT 
AAAGATTCAG AGGGCTCTGG AACTGCTGGG AAAACAGGGA GTTTAGGAAA AAAACCTGAA 
ATTGCCCAGG TTATTGCCTC ATACACCGCC ACCGGCCCCG AGCAGCTCAC TCTCGCCCCT 
GGTCAGCTGA TTTTGATCCG AAAAAAGAAC CCAGGTGGAT GGTGGGAAGG AGAGCTGCAA 
GCACGTGGGA AAAAGCGCCA GATAGGCTGG TTCCCAGCTA ATTATGTAAA GCTTCTAAGC 
CCTGGGACGA GCAAAATCAC TCCAACAGAG CCACCTAAGT CAACAGCATT AGCGGCAGTG 
TGCCAGGTGA TTGGGATGTA CGACTACACC GCGCAGAATG ACGATGAGCT GGCCTTCAAC 
AAGGGCCAGA TCATCAACGT CCTCAACAAG GAGGACCCTG ACTGGTGGAA AGGAGAAGTC 
AATGGACAAG TGGGGCTCTT CCCATCCAAT TATGTGAAGC TGACCACAGA CATGGACCCA 
AGCCAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883927.1
CDS	295..3846
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	XM_017028438.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028438.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAATTT ATTGCCATGT ACACTTACGA GAGTTCTGAG 
CAAGGAGATT TAACCTTTCA GCAAGGGGAT GTGATTTTGG TTACCAAGAA AGATGGTGAC 
TGGTGGACAG GAACAGTGGG CGACAAGGCC GGAGTCTTCC CTTCTAACTA TGTGAGGCTT 
AAAGATTCAG AGGGCTCTGG AACTGCTGGG AAAACAGGGA GTTTAGGAAA AAAACCTGAA 
ATTGCCCAGG TTATTGCCTC ATACACCGCC ACCGGCCCCG AGCAGCTCAC TCTCGCCCCT 
GGTCAGCTGA TTTTGATCCG AAAAAAGAAC CCAGGTGGAT GGTGGGAAGG AGAGCTGCAA 
GCACGTGGGA AAAAGCGCCA GATAGGCTGG TTCCCAGCTA ATTATGTAAA GCTTCTAAGC 
CCTGGGACGA GCAAAATCAC TCCAACAGAG CCACCTAAGT CAACAGCATT AGCGGCAGTG 
TGCCAGGTGA TTGGGATGTA CGACTACACC GCGCAGAATG ACGATGAGCT GGCCTTCAAC 
AAGGGCCAGA TCATCAACGT CCTCAACAAG GAGGACCCTG ACTGGTGGAA AGGAGAAGTC 
AATGGACAAG TGGGGCTCTT CCCATCCAAT TATGTGAAGC TGACCACAGA CATGGACCCA 
AGCCAGCAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028440.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883929.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X17, mRNA.

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CloneID	OHu93303
Clone ID Related Accession (Same CDS sequence)	XM_017028440.1 , XM_017028440.2
Accession Version	XM_017028440.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAAATT GCCCAGGTTA TTGCCTCATA CACCGCCACC 
GGCCCCGAGC AGCTCACTCT CGCCCCTGGT CAGCTGATTT TGATCCGAAA AAAGAACCCA 
GGTGGATGGT GGGAAGGAGA GCTGCAAGCA CGTGGGAAAA AGCGCCAGAT AGGCTGGTTC 
CCAGCTAATT ATGTAAAGCT TCTAAGCCCT GGGACGAGCA AAATCACTCC AACAGAGCCA 
CCTAAGTCAA CAGCATTAGC GGCAGTGTGC CAGGTGATTG GGATGTACGA CTACACCGCG 
CAGAATGACG ATGAGCTGGC CTTCAACAAG GGCCAGATCA TCAACGTCCT CAACAAGGAG 
GACCCTGACT GGTGGAAAGG AGAAGTCAAT GGACAAGTGG GGCTCTTCCC ATCCAATTAT 
GTGAAGCTGA CCACAGACAT GGACCCAAGC CAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883929.1
CDS	347..3685
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	XM_017028440.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028440.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAAATT GCCCAGGTTA TTGCCTCATA CACCGCCACC 
GGCCCCGAGC AGCTCACTCT CGCCCCTGGT CAGCTGATTT TGATCCGAAA AAAGAACCCA 
GGTGGATGGT GGGAAGGAGA GCTGCAAGCA CGTGGGAAAA AGCGCCAGAT AGGCTGGTTC 
CCAGCTAATT ATGTAAAGCT TCTAAGCCCT GGGACGAGCA AAATCACTCC AACAGAGCCA 
CCTAAGTCAA CAGCATTAGC GGCAGTGTGC CAGGTGATTG GGATGTACGA CTACACCGCG 
CAGAATGACG ATGAGCTGGC CTTCAACAAG GGCCAGATCA TCAACGTCCT CAACAAGGAG 
GACCCTGACT GGTGGAAAGG AGAAGTCAAT GGACAAGTGG GGCTCTTCCC ATCCAATTAT 
GTGAAGCTGA CCACAGACAT GGACCCAAGC CAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028440.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883929.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OHu93303
Clone ID Related Accession (Same CDS sequence)	XM_017028440.1 , XM_017028440.2
Accession Version	XM_017028440.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3339bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028440.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAAATT GCCCAGGTTA TTGCCTCATA CACCGCCACC 
GGCCCCGAGC AGCTCACTCT CGCCCCTGGT CAGCTGATTT TGATCCGAAA AAAGAACCCA 
GGTGGATGGT GGGAAGGAGA GCTGCAAGCA CGTGGGAAAA AGCGCCAGAT AGGCTGGTTC 
CCAGCTAATT ATGTAAAGCT TCTAAGCCCT GGGACGAGCA AAATCACTCC AACAGAGCCA 
CCTAAGTCAA CAGCATTAGC GGCAGTGTGC CAGGTGATTG GGATGTACGA CTACACCGCG 
CAGAATGACG ATGAGCTGGC CTTCAACAAG GGCCAGATCA TCAACGTCCT CAACAAGGAG 
GACCCTGACT GGTGGAAAGG AGAAGTCAAT GGACAAGTGG GGCTCTTCCC ATCCAATTAT 
GTGAAGCTGA CCACAGACAT GGACCCAAGC CAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883929.1
CDS	295..3633
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	XM_017028440.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028440.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAAATT GCCCAGGTTA TTGCCTCATA CACCGCCACC 
GGCCCCGAGC AGCTCACTCT CGCCCCTGGT CAGCTGATTT TGATCCGAAA AAAGAACCCA 
GGTGGATGGT GGGAAGGAGA GCTGCAAGCA CGTGGGAAAA AGCGCCAGAT AGGCTGGTTC 
CCAGCTAATT ATGTAAAGCT TCTAAGCCCT GGGACGAGCA AAATCACTCC AACAGAGCCA 
CCTAAGTCAA CAGCATTAGC GGCAGTGTGC CAGGTGATTG GGATGTACGA CTACACCGCG 
CAGAATGACG ATGAGCTGGC CTTCAACAAG GGCCAGATCA TCAACGTCCT CAACAAGGAG 
GACCCTGACT GGTGGAAAGG AGAAGTCAAT GGACAAGTGG GGCTCTTCCC ATCCAATTAT 
GTGAAGCTGA CCACAGACAT GGACCCAAGC CAGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028441.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883930.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu93304
Clone ID Related Accession (Same CDS sequence)	XM_017028441.2 , XM_017028441.1
Accession Version	XM_017028441.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028441.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGCCT GACTTCCTCC TCCACCCCTC CATGAGACTA 
GGCCACATGC AGCCCCGGAT TGTGCTTCTC TTCCCGGACC CCCTGCAGTG TTCCACCTCG 
AGGCTTTTGC CCATGCTGCG CCCTCGGCCT GGAGTGCCCT TCCTCCGAAG CCCTAGCTGT 
CAAAGTCCTT CTCATCCTTC AAGGCCCATC TCAGATGCTG CCCCCTCTGT GAAATTTACG 
CTGATGCCCC CAGGCAGAAT TCATCCCTGT TTTCTCTTTA TTCCTGCAGT GAATAGTAGG 
AACTCCTTTT TAGTATATTT CATTCTGCCA GGTGGTACAC TTGGTTGTTT TTATCTCTGT 
CTTCCCCATT ACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883930.1
CDS	294..3371
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQPDFLLHPSMRLGHMQPRIVLLFPDPLQCSTSRLLPMLRPRPGVPFLRSPSCQSPSHPSRPISDAAPSVKFTLMPPGRIHPCFLFIPAVNSRNSFLVYFILPGGTLGCFYLCLPHYL

Target ORF information:

RefSeq Version	XM_017028441.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028441.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGCCT GACTTCCTCC TCCACCCCTC CATGAGACTA 
GGCCACATGC AGCCCCGGAT TGTGCTTCTC TTCCCGGACC CCCTGCAGTG TTCCACCTCG 
AGGCTTTTGC CCATGCTGCG CCCTCGGCCT GGAGTGCCCT TCCTCCGAAG CCCTAGCTGT 
CAAAGTCCTT CTCATCCTTC AAGGCCCATC TCAGATGCTG CCCCCTCTGT GAAATTTACG 
CTGATGCCCC CAGGCAGAAT TCATCCCTGT TTTCTCTTTA TTCCTGCAGT GAATAGTAGG 
AACTCCTTTT TAGTATATTT CATTCTGCCA GGTGGTACAC TTGGTTGTTT TTATCTCTGT 
CTTCCCCATT ACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028441.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883930.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X19, mRNA.

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CloneID	OHu93304
Clone ID Related Accession (Same CDS sequence)	XM_017028441.2 , XM_017028441.1
Accession Version	XM_017028441.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3078bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGCCT GACTTCCTCC TCCACCCCTC CATGAGACTA 
GGCCACATGC AGCCCCGGAT TGTGCTTCTC TTCCCGGACC CCCTGCAGTG TTCCACCTCG 
AGGCTTTTGC CCATGCTGCG CCCTCGGCCT GGAGTGCCCT TCCTCCGAAG CCCTAGCTGT 
CAAAGTCCTT CTCATCCTTC AAGGCCCATC TCAGATGCTG CCCCCTCTGT GAAATTTACG 
CTGATGCCCC CAGGCAGAAT TCATCCCTGT TTTCTCTTTA TTCCTGCAGT GAATAGTAGG 
AACTCCTTTT TAGTATATTT CATTCTGCCA GGTGGTACAC TTGGTTGTTT TTATCTCTGT 
CTTCCCCATT ACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883930.1
CDS	347..3424
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQPDFLLHPSMRLGHMQPRIVLLFPDPLQCSTSRLLPMLRPRPGVPFLRSPSCQSPSHPSRPISDAAPSVKFTLMPPGRIHPCFLFIPAVNSRNSFLVYFILPGGTLGCFYLCLPHYL

Target ORF information:

RefSeq Version	XM_017028441.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028441.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGCCT GACTTCCTCC TCCACCCCTC CATGAGACTA 
GGCCACATGC AGCCCCGGAT TGTGCTTCTC TTCCCGGACC CCCTGCAGTG TTCCACCTCG 
AGGCTTTTGC CCATGCTGCG CCCTCGGCCT GGAGTGCCCT TCCTCCGAAG CCCTAGCTGT 
CAAAGTCCTT CTCATCCTTC AAGGCCCATC TCAGATGCTG CCCCCTCTGT GAAATTTACG 
CTGATGCCCC CAGGCAGAAT TCATCCCTGT TTTCTCTTTA TTCCTGCAGT GAATAGTAGG 
AACTCCTTTT TAGTATATTT CATTCTGCCA GGTGGTACAC TTGGTTGTTT TTATCTCTGT 
CTTCCCCATT ACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( NM_001001132.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001001132.1 Homo sapiens intersectin 1 (ITSN1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10132
Clone ID Related Accession (Same CDS sequence)	NM_001001132.1 , NM_001001132.2
Accession Version	NM_001001132.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	intersectin-1 isoform ITSN-s
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058925.1, AF114488.1 and AI857585.1. Transcript Variant: This variant (2) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (ITSN-s) is shorter at the C-terminus compared to isoform ITSN-l. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF114488.1, SRR1803617.192420.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001132.1
CDS	269..3931
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	NM_001001132.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001132.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( NM_001001132.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001001132.1 Homo sapiens intersectin 1 (ITSN1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10132
Clone ID Related Accession (Same CDS sequence)	NM_001001132.1 , NM_001001132.2
Accession Version	NM_001001132.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3663bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	intersectin-1 isoform ITSN-s
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058925.1, AF114488.1 and AI857585.1. On May 31, 2019 this sequence version replaced NM_001001132.1. Transcript Variant: This variant (2) differs in the 3' UTR and coding region compared to variant 1. The resulting isoform (ITSN-s) is shorter at the C-terminus compared to isoform ITSN-l. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF114488.1, SRR1803615.33040.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2154665 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001001132.1
CDS	246..3908
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	NM_001001132.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001001132.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( NM_001331009.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317938.1 Homo sapiens intersectin 1 (ITSN1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu53297
Clone ID Related Accession (Same CDS sequence)	NM_001331009.2 , NM_001331009.1
Accession Version	NM_001331009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	intersectin-1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK027846.1, AK300274.1, AP000312.2 and CX163355.1. On May 31, 2019 this sequence version replaced NM_001331009.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.131293.1, SRR1803615.98909.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GAGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGGCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CCCAGGTTAT TGCCTCATAC 
ACCGCCACCG GCCCCGAGCA GCTCACTCTC GCCCCTGGTC AGCTGATTTT GATCCGAAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTGCAAGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTCC CAGCTAATTA TGTAAAGCTT CTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGAGCCAC CTAAGTCAAC AGCATTAGCG GCAGTGTGCC AGGTGATTGG GATGTACGAC 
TACACCGCGC AGAATGACGA TGAGCTGGCC TTCAACAAGG GCCAGATCAT CAACGTCCTC 
AACAAGGAGG ACCCTGACTG GTGGAAAGGA GAAGTCAATG GACAAGTGGG GCTCTTCCCA 
TCCAATTATG TGAAGCTGAC CACAGACATG GACCCAAGCC AGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317938.1
CDS	246..3893
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	NM_001331009.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331009.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GAGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGGCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CCCAGGTTAT TGCCTCATAC 
ACCGCCACCG GCCCCGAGCA GCTCACTCTC GCCCCTGGTC AGCTGATTTT GATCCGAAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTGCAAGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTCC CAGCTAATTA TGTAAAGCTT CTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGAGCCAC CTAAGTCAAC AGCATTAGCG GCAGTGTGCC AGGTGATTGG GATGTACGAC 
TACACCGCGC AGAATGACGA TGAGCTGGCC TTCAACAAGG GCCAGATCAT CAACGTCCTC 
AACAAGGAGG ACCCTGACTG GTGGAAAGGA GAAGTCAATG GACAAGTGGG GCTCTTCCCA 
TCCAATTATG TGAAGCTGAC CACAGACATG GACCCAAGCC AGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( NM_001331009.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317938.1 Homo sapiens intersectin 1 (ITSN1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu53297
Clone ID Related Accession (Same CDS sequence)	NM_001331009.2 , NM_001331009.1
Accession Version	NM_001331009.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	intersectin-1 isoform 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK027846.1, AK300274.1, AP000312.2 and CX163355.1. On Sep 3, 2016 this sequence version replaced XM_005261027.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.131293.1, SRR1803612.279661.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GAGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGGCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CCCAGGTTAT TGCCTCATAC 
ACCGCCACCG GCCCCGAGCA GCTCACTCTC GCCCCTGGTC AGCTGATTTT GATCCGAAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTGCAAGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTCC CAGCTAATTA TGTAAAGCTT CTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGAGCCAC CTAAGTCAAC AGCATTAGCG GCAGTGTGCC AGGTGATTGG GATGTACGAC 
TACACCGCGC AGAATGACGA TGAGCTGGCC TTCAACAAGG GCCAGATCAT CAACGTCCTC 
AACAAGGAGG ACCCTGACTG GTGGAAAGGA GAAGTCAATG GACAAGTGGG GCTCTTCCCA 
TCCAATTATG TGAAGCTGAC CACAGACATG GACCCAAGCC AGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317938.1
CDS	347..3994
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	NM_001331009.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331009.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GAGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGGCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CCCAGGTTAT TGCCTCATAC 
ACCGCCACCG GCCCCGAGCA GCTCACTCTC GCCCCTGGTC AGCTGATTTT GATCCGAAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTGCAAGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTCC CAGCTAATTA TGTAAAGCTT CTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGAGCCAC CTAAGTCAAC AGCATTAGCG GCAGTGTGCC AGGTGATTGG GATGTACGAC 
TACACCGCGC AGAATGACGA TGAGCTGGCC TTCAACAAGG GCCAGATCAT CAACGTCCTC 
AACAAGGAGG ACCCTGACTG GTGGAAAGGA GAAGTCAATG GACAAGTGGG GCTCTTCCCA 
TCCAATTATG TGAAGCTGAC CACAGACATG GACCCAAGCC AGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( NM_001331011.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317940.1 Homo sapiens intersectin 1 (ITSN1), transcript variant 6, mRNA.

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CloneID	OHu53298
Clone ID Related Accession (Same CDS sequence)	NM_001331011.1 , NM_001331011.2
Accession Version	NM_001331011.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	intersectin-1 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK027846.1, BC117560.1, AP000312.2 and CX163355.1. On Sep 3, 2016 this sequence version replaced XM_005261031.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.50207.1, SRR1660809.238493.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAAATTGCCC AGGTTATTGC CTCATACACC GCCACCGGCC CCGAGCAGCT CACTCTCGCC 
CCTGGTCAGC TGATTTTGAT CCGAAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTG 
CAAGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTCCCAG CTAATTATGT AAAGCTTCTA 
AGCCCTGGGA CGAGCAAAAT CACTCCAACA GAGCCACCTA AGTCAACAGC ATTAGCGGCA 
GTGTGCCAGG TGATTGGGAT GTACGACTAC ACCGCGCAGA ATGACGATGA GCTGGCCTTC 
AACAAGGGCC AGATCATCAA CGTCCTCAAC AAGGAGGACC CTGACTGGTG GAAAGGAGAA 
GTCAATGGAC AAGTGGGGCT CTTCCCATCC AATTATGTGA AGCTGACCAC AGACATGGAC 
CCAAGCCAGC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317940.1
CDS	347..3781
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	NM_001331011.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331011.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAAATTGCCC AGGTTATTGC CTCATACACC GCCACCGGCC CCGAGCAGCT CACTCTCGCC 
CCTGGTCAGC TGATTTTGAT CCGAAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTG 
CAAGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTCCCAG CTAATTATGT AAAGCTTCTA 
AGCCCTGGGA CGAGCAAAAT CACTCCAACA GAGCCACCTA AGTCAACAGC ATTAGCGGCA 
GTGTGCCAGG TGATTGGGAT GTACGACTAC ACCGCGCAGA ATGACGATGA GCTGGCCTTC 
AACAAGGGCC AGATCATCAA CGTCCTCAAC AAGGAGGACC CTGACTGGTG GAAAGGAGAA 
GTCAATGGAC AAGTGGGGCT CTTCCCATCC AATTATGTGA AGCTGACCAC AGACATGGAC 
CCAAGCCAGC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( NM_001331011.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317940.1 Homo sapiens intersectin 1 (ITSN1), transcript variant 6, mRNA.

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CloneID	OHu53298
Clone ID Related Accession (Same CDS sequence)	NM_001331011.1 , NM_001331011.2
Accession Version	NM_001331011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	intersectin-1 isoform 6
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC117560.1, AP000312.2 and CX163355.1. On May 31, 2019 this sequence version replaced NM_001331011.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.22035.1, SRR1660809.238493.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAAATTGCCC AGGTTATTGC CTCATACACC GCCACCGGCC CCGAGCAGCT CACTCTCGCC 
CCTGGTCAGC TGATTTTGAT CCGAAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTG 
CAAGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTCCCAG CTAATTATGT AAAGCTTCTA 
AGCCCTGGGA CGAGCAAAAT CACTCCAACA GAGCCACCTA AGTCAACAGC ATTAGCGGCA 
GTGTGCCAGG TGATTGGGAT GTACGACTAC ACCGCGCAGA ATGACGATGA GCTGGCCTTC 
AACAAGGGCC AGATCATCAA CGTCCTCAAC AAGGAGGACC CTGACTGGTG GAAAGGAGAA 
GTCAATGGAC AAGTGGGGCT CTTCCCATCC AATTATGTGA AGCTGACCAC AGACATGGAC 
CCAAGCCAGC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317940.1
CDS	246..3680
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	NM_001331011.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331011.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAAATTGCCC AGGTTATTGC CTCATACACC GCCACCGGCC CCGAGCAGCT CACTCTCGCC 
CCTGGTCAGC TGATTTTGAT CCGAAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTG 
CAAGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTCCCAG CTAATTATGT AAAGCTTCTA 
AGCCCTGGGA CGAGCAAAAT CACTCCAACA GAGCCACCTA AGTCAACAGC ATTAGCGGCA 
GTGTGCCAGG TGATTGGGAT GTACGACTAC ACCGCGCAGA ATGACGATGA GCTGGCCTTC 
AACAAGGGCC AGATCATCAA CGTCCTCAAC AAGGAGGACC CTGACTGGTG GAAAGGAGAA 
GTCAATGGAC AAGTGGGGCT CTTCCCATCC AATTATGTGA AGCTGACCAC AGACATGGAC 
CCAAGCCAGC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( NM_001331012.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317941.1 Homo sapiens intersectin 1 (ITSN1), transcript variant 7, mRNA.

Price & Availability↑

CloneID	OHu60474
Clone ID Related Accession (Same CDS sequence)	NM_001331012.1 , NM_001331012.2
Accession Version	NM_001331012.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-02
Organism	Homo sapiens(human)
Product	intersectin-1 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK027846.1, AP000308.1, AP000311.1, KF511399.1, AP000312.2 and CX163355.1. On Sep 3, 2016 this sequence version replaced XM_011529691.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.252261.1, DQ679756.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AATTTATTGC CATGTACACT TACGAGAGTT CTGAGCAAGG AGATTTAACC 
TTTCAGCAAG GGGATGTGAT TTTGGTTACC AAGAAAGATG GTGACTGGTG GACAGGAACA 
GTGGGCGACA AGGCCGGAGT CTTCCCTTCT AACTATGTGA GGCTTAAAGA TTCAGAGGGC 
TCTGGAACTG CTGGGAAAAC AGGGAGTTTA GGAAAAAAAC CTGAAATTGC CCAGGTTATT 
GCCTCATACA CCGCCACCGG CCCCGAGCAG CTCACTCTCG CCCCTGGTCA GCTGATTTTG 
ATCCGAAAAA AGAACCCAGG TGGATGGTGG GAAGGAGAGC TGCAAGCACG TGGGAAAAAG 
CGCCAGATAG GCTGGTTCCC AGCTAATTAT GTAAAGCTTC TAAGCCCTGG GACGAGCAAA 
ATCACTCCAA CAGAGCCACC TAAGTCAACA GCATTAGCGG CAGTGTGCCA GGTGATTGGG 
ATGTACGACT ACACCGCGCA GAATGACGAT GAGCTGGCCT TCAACAAGGG CCAGATCATC 
AACGTCCTCA ACAAGGAGGA CCCTGACTGG TGGAAAGGAG AAGTCAATGG ACAAGTGGGG 
CTCTTCCCAT CCAATTATGT GAAGCTGACC ACAGACATGG ACCCAAGCCA GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317941.1
CDS	347..3883
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	NM_001331012.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331012.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AATTTATTGC CATGTACACT TACGAGAGTT CTGAGCAAGG AGATTTAACC 
TTTCAGCAAG GGGATGTGAT TTTGGTTACC AAGAAAGATG GTGACTGGTG GACAGGAACA 
GTGGGCGACA AGGCCGGAGT CTTCCCTTCT AACTATGTGA GGCTTAAAGA TTCAGAGGGC 
TCTGGAACTG CTGGGAAAAC AGGGAGTTTA GGAAAAAAAC CTGAAATTGC CCAGGTTATT 
GCCTCATACA CCGCCACCGG CCCCGAGCAG CTCACTCTCG CCCCTGGTCA GCTGATTTTG 
ATCCGAAAAA AGAACCCAGG TGGATGGTGG GAAGGAGAGC TGCAAGCACG TGGGAAAAAG 
CGCCAGATAG GCTGGTTCCC AGCTAATTAT GTAAAGCTTC TAAGCCCTGG GACGAGCAAA 
ATCACTCCAA CAGAGCCACC TAAGTCAACA GCATTAGCGG CAGTGTGCCA GGTGATTGGG 
ATGTACGACT ACACCGCGCA GAATGACGAT GAGCTGGCCT TCAACAAGGG CCAGATCATC 
AACGTCCTCA ACAAGGAGGA CCCTGACTGG TGGAAAGGAG AAGTCAATGG ACAAGTGGGG 
CTCTTCCCAT CCAATTATGT GAAGCTGACC ACAGACATGG ACCCAAGCCA GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( NM_001331012.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317941.1 Homo sapiens intersectin 1 (ITSN1), transcript variant 7, mRNA.

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CloneID	OHu60474
Clone ID Related Accession (Same CDS sequence)	NM_001331012.1 , NM_001331012.2
Accession Version	NM_001331012.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-01
Organism	Homo sapiens(human)
Product	intersectin-1 isoform 7
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK027846.1, AP000308.1, AP000311.1, KF511399.1, AP000312.2 and CX163355.1. On Jun 1, 2019 this sequence version replaced NM_001331012.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.252261.1, DQ679756.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AATTTATTGC CATGTACACT TACGAGAGTT CTGAGCAAGG AGATTTAACC 
TTTCAGCAAG GGGATGTGAT TTTGGTTACC AAGAAAGATG GTGACTGGTG GACAGGAACA 
GTGGGCGACA AGGCCGGAGT CTTCCCTTCT AACTATGTGA GGCTTAAAGA TTCAGAGGGC 
TCTGGAACTG CTGGGAAAAC AGGGAGTTTA GGAAAAAAAC CTGAAATTGC CCAGGTTATT 
GCCTCATACA CCGCCACCGG CCCCGAGCAG CTCACTCTCG CCCCTGGTCA GCTGATTTTG 
ATCCGAAAAA AGAACCCAGG TGGATGGTGG GAAGGAGAGC TGCAAGCACG TGGGAAAAAG 
CGCCAGATAG GCTGGTTCCC AGCTAATTAT GTAAAGCTTC TAAGCCCTGG GACGAGCAAA 
ATCACTCCAA CAGAGCCACC TAAGTCAACA GCATTAGCGG CAGTGTGCCA GGTGATTGGG 
ATGTACGACT ACACCGCGCA GAATGACGAT GAGCTGGCCT TCAACAAGGG CCAGATCATC 
AACGTCCTCA ACAAGGAGGA CCCTGACTGG TGGAAAGGAG AAGTCAATGG ACAAGTGGGG 
CTCTTCCCAT CCAATTATGT GAAGCTGACC ACAGACATGG ACCCAAGCCA GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317941.1
CDS	246..3782
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	NM_001331012.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331012.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AATTTATTGC CATGTACACT TACGAGAGTT CTGAGCAAGG AGATTTAACC 
TTTCAGCAAG GGGATGTGAT TTTGGTTACC AAGAAAGATG GTGACTGGTG GACAGGAACA 
GTGGGCGACA AGGCCGGAGT CTTCCCTTCT AACTATGTGA GGCTTAAAGA TTCAGAGGGC 
TCTGGAACTG CTGGGAAAAC AGGGAGTTTA GGAAAAAAAC CTGAAATTGC CCAGGTTATT 
GCCTCATACA CCGCCACCGG CCCCGAGCAG CTCACTCTCG CCCCTGGTCA GCTGATTTTG 
ATCCGAAAAA AGAACCCAGG TGGATGGTGG GAAGGAGAGC TGCAAGCACG TGGGAAAAAG 
CGCCAGATAG GCTGGTTCCC AGCTAATTAT GTAAAGCTTC TAAGCCCTGG GACGAGCAAA 
ATCACTCCAA CAGAGCCACC TAAGTCAACA GCATTAGCGG CAGTGTGCCA GGTGATTGGG 
ATGTACGACT ACACCGCGCA GAATGACGAT GAGCTGGCCT TCAACAAGGG CCAGATCATC 
AACGTCCTCA ACAAGGAGGA CCCTGACTGG TGGAAAGGAG AAGTCAATGG ACAAGTGGGG 
CTCTTCCCAT CCAATTATGT GAAGCTGACC ACAGACATGG ACCCAAGCCA GCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_011529692.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527994.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OHu60475
Clone ID Related Accession (Same CDS sequence)	XM_011529692.1 , XM_011529692.2
Accession Version	XM_011529692.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AAATTGCCCA GGTTATTGCC TCATACACCG CCACCGGCCC CGAGCAGCTC 
ACTCTCGCCC CTGGTCAGCT GATTTTGATC CGAAAAAAGA ACCCAGGTGG ATGGTGGGAA 
GGAGAGCTGC AAGCACGTGG GAAAAAGCGC CAGATAGGCT GGTTCCCAGC TAATTATGTA 
AAGCTTCTAA GCCCTGGGAC GAGCAAAATC ACTCCAACAG AGCCACCTAA GTCAACAGCA 
TTAGCGGCAG TGTGCCAGGT GATTGGGATG TACGACTACA CCGCGCAGAA TGACGATGAG 
CTGGCCTTCA ACAAGGGCCA GATCATCAAC GTCCTCAACA AGGAGGACCC TGACTGGTGG 
AAAGGAGAAG TCAATGGACA AGTGGGGCTC TTCCCATCCA ATTATGTGAA GCTGACCACA 
GACATGGACC CAAGCCAGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527994.1
CDS	347..3670
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	XM_011529692.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529692.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AAATTGCCCA GGTTATTGCC TCATACACCG CCACCGGCCC CGAGCAGCTC 
ACTCTCGCCC CTGGTCAGCT GATTTTGATC CGAAAAAAGA ACCCAGGTGG ATGGTGGGAA 
GGAGAGCTGC AAGCACGTGG GAAAAAGCGC CAGATAGGCT GGTTCCCAGC TAATTATGTA 
AAGCTTCTAA GCCCTGGGAC GAGCAAAATC ACTCCAACAG AGCCACCTAA GTCAACAGCA 
TTAGCGGCAG TGTGCCAGGT GATTGGGATG TACGACTACA CCGCGCAGAA TGACGATGAG 
CTGGCCTTCA ACAAGGGCCA GATCATCAAC GTCCTCAACA AGGAGGACCC TGACTGGTGG 
AAAGGAGAAG TCAATGGACA AGTGGGGCTC TTCCCATCCA ATTATGTGAA GCTGACCACA 
GACATGGACC CAAGCCAGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_011529692.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527994.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OHu60475
Clone ID Related Accession (Same CDS sequence)	XM_011529692.1 , XM_011529692.2
Accession Version	XM_011529692.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011529692.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AAATTGCCCA GGTTATTGCC TCATACACCG CCACCGGCCC CGAGCAGCTC 
ACTCTCGCCC CTGGTCAGCT GATTTTGATC CGAAAAAAGA ACCCAGGTGG ATGGTGGGAA 
GGAGAGCTGC AAGCACGTGG GAAAAAGCGC CAGATAGGCT GGTTCCCAGC TAATTATGTA 
AAGCTTCTAA GCCCTGGGAC GAGCAAAATC ACTCCAACAG AGCCACCTAA GTCAACAGCA 
TTAGCGGCAG TGTGCCAGGT GATTGGGATG TACGACTACA CCGCGCAGAA TGACGATGAG 
CTGGCCTTCA ACAAGGGCCA GATCATCAAC GTCCTCAACA AGGAGGACCC TGACTGGTGG 
AAAGGAGAAG TCAATGGACA AGTGGGGCTC TTCCCATCCA ATTATGTGAA GCTGACCACA 
GACATGGACC CAAGCCAGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527994.1
CDS	295..3618
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQ

Target ORF information:

RefSeq Version	XM_011529692.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529692.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AAATTGCCCA GGTTATTGCC TCATACACCG CCACCGGCCC CGAGCAGCTC 
ACTCTCGCCC CTGGTCAGCT GATTTTGATC CGAAAAAAGA ACCCAGGTGG ATGGTGGGAA 
GGAGAGCTGC AAGCACGTGG GAAAAAGCGC CAGATAGGCT GGTTCCCAGC TAATTATGTA 
AAGCTTCTAA GCCCTGGGAC GAGCAAAATC ACTCCAACAG AGCCACCTAA GTCAACAGCA 
TTAGCGGCAG TGTGCCAGGT GATTGGGATG TACGACTACA CCGCGCAGAA TGACGATGAG 
CTGGCCTTCA ACAAGGGCCA GATCATCAAC GTCCTCAACA AGGAGGACCC TGACTGGTGG 
AAAGGAGAAG TCAATGGACA AGTGGGGCTC TTCCCATCCA ATTATGTGAA GCTGACCACA 
GACATGGACC CAAGCCAGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_011529693.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527995.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X21, mRNA.

Price & Availability↑

CloneID	OHu60476
Clone ID Related Accession (Same CDS sequence)	XM_011529693.2 , XM_011529693.3
Accession Version	XM_011529693.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011529693.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGCCTGACTT CCTCCTCCAC CCCTCCATGA GACTAGGCCA CATGCAGCCC 
CGGATTGTGC TTCTCTTCCC GGACCCCCTG CAGTGTTCCA CCTCGAGGCT TTTGCCCATG 
CTGCGCCCTC GGCCTGGAGT GCCCTTCCTC CGAAGCCCTA GCTGTCAAAG TCCTTCTCAT 
CCTTCAAGGC CCATCTCAGA TGCTGCCCCC TCTGTGAAAT TTACGCTGAT GCCCCCAGGC 
AGAATTCATC CCTGTTTTCT CTTTATTCCT GCAGTGAATA GTAGGAACTC CTTTTTAGTA 
TATTTCATTC TGCCAGGTGG TACACTTGGT TGTTTTTATC TCTGTCTTCC CCATTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527995.1
CDS	347..3409
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQPDFLLHPSMRLGHMQPRIVLLFPDPLQCSTSRLLPMLRPRPGVPFLRSPSCQSPSHPSRPISDAAPSVKFTLMPPGRIHPCFLFIPAVNSRNSFLVYFILPGGTLGCFYLCLPHYL

Target ORF information:

RefSeq Version	XM_011529693.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529693.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGCCTGACTT CCTCCTCCAC CCCTCCATGA GACTAGGCCA CATGCAGCCC 
CGGATTGTGC TTCTCTTCCC GGACCCCCTG CAGTGTTCCA CCTCGAGGCT TTTGCCCATG 
CTGCGCCCTC GGCCTGGAGT GCCCTTCCTC CGAAGCCCTA GCTGTCAAAG TCCTTCTCAT 
CCTTCAAGGC CCATCTCAGA TGCTGCCCCC TCTGTGAAAT TTACGCTGAT GCCCCCAGGC 
AGAATTCATC CCTGTTTTCT CTTTATTCCT GCAGTGAATA GTAGGAACTC CTTTTTAGTA 
TATTTCATTC TGCCAGGTGG TACACTTGGT TGTTTTTATC TCTGTCTTCC CCATTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_011529693.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527995.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X16, mRNA.

Price & Availability↑

CloneID	OHu60476
Clone ID Related Accession (Same CDS sequence)	XM_011529693.2 , XM_011529693.3
Accession Version	XM_011529693.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011529693.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGCCTGACTT CCTCCTCCAC CCCTCCATGA GACTAGGCCA CATGCAGCCC 
CGGATTGTGC TTCTCTTCCC GGACCCCCTG CAGTGTTCCA CCTCGAGGCT TTTGCCCATG 
CTGCGCCCTC GGCCTGGAGT GCCCTTCCTC CGAAGCCCTA GCTGTCAAAG TCCTTCTCAT 
CCTTCAAGGC CCATCTCAGA TGCTGCCCCC TCTGTGAAAT TTACGCTGAT GCCCCCAGGC 
AGAATTCATC CCTGTTTTCT CTTTATTCCT GCAGTGAATA GTAGGAACTC CTTTTTAGTA 
TATTTCATTC TGCCAGGTGG TACACTTGGT TGTTTTTATC TCTGTCTTCC CCATTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527995.1
CDS	294..3356
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQPDFLLHPSMRLGHMQPRIVLLFPDPLQCSTSRLLPMLRPRPGVPFLRSPSCQSPSHPSRPISDAAPSVKFTLMPPGRIHPCFLFIPAVNSRNSFLVYFILPGGTLGCFYLCLPHYL

Target ORF information:

RefSeq Version	XM_011529693.3
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011529693.3

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGCCTGACTT CCTCCTCCAC CCCTCCATGA GACTAGGCCA CATGCAGCCC 
CGGATTGTGC TTCTCTTCCC GGACCCCCTG CAGTGTTCCA CCTCGAGGCT TTTGCCCATG 
CTGCGCCCTC GGCCTGGAGT GCCCTTCCTC CGAAGCCCTA GCTGTCAAAG TCCTTCTCAT 
CCTTCAAGGC CCATCTCAGA TGCTGCCCCC TCTGTGAAAT TTACGCTGAT GCCCCCAGGC 
AGAATTCATC CCTGTTTTCT CTTTATTCCT GCAGTGAATA GTAGGAACTC CTTTTTAGTA 
TATTTCATTC TGCCAGGTGG TACACTTGGT TGTTTTTATC TCTGTCTTCC CCATTACTTG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028429.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883918.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X3, mRNA.

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CloneID	OHu93293
Clone ID Related Accession (Same CDS sequence)	XM_017028429.1 , XM_017028429.2
Accession Version	XM_017028429.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAATTT ATTGCCATGT ACACTTACGA GAGTTCTGAG 
CAAGGAGATT TAACCTTTCA GCAAGGGGAT GTGATTTTGG TTACCAAGAA AGATGGTGAC 
TGGTGGACAG GAACAGTGGG CGACAAGGCC GGAGTCTTCC CTTCTAACTA TGTGAGGCTT 
AAAGATTCAG AGGGCTCTGG AACTGCTGGG AAAACAGGGA GTTTAGGAAA AAAACCTGAA 
ATTGCCCAGG TTATTGCCTC ATACACCGCC ACCGGCCCCG AGCAGCTCAC TCTCGCCCCT 
GGTCAGCTGA TTTTGATCCG AAAAAAGAAC CCAGGTGGAT GGTGGGAAGG AGAGCTGCAA 
GCACGTGGGA AAAAGCGCCA GATAGGCTGG TTCCCAGCTA ATTATGTAAA GCTTCTAAGC 
CCTGGGACGA GCAAAATCAC TCCAACAGAG CCACCTAAGT CAACAGCATT AGCGGCAGTG 
TGCCAGGTGA TTGGGATGTA CGACTACACC GCGCAGAATG ACGATGAGCT GGCCTTCAAC 
AAGGGCCAGA TCATCAACGT CCTCAACAAG GAGGACCCTG ACTGGTGGAA AGGAGAAGTC 
AATGGACAAG TGGGGCTCTT CCCATCCAAT TATGTGAAGC TGACCACAGA CATGGACCCA 
AGCCAGCAAT GGTGTTCAGA CTTACATCTC TTGGATATGT TGACCCCAAC TGAAAGAAAG 
CGACAAGGAT ACATCCACGA GCTCATTGTC ACCGAGGAGA ACTATGTGAA TGACCTGCAG 
CTGGTCACAG AGATTTTTCA AAAACCCCTG ATGGAGTCTG AGCTGCTGAC AGAAAAAGAG 
GTTGCTATGA TTTTTGTGAA CTGGAAGGAG CTGATTATGT GTAATATCAA ACTACTAAAA 
GCGCTGAGAG TCCGCAAGAA GATGTCCGGG GAGAAGATGC CTGTGAAGAT GATTGGAGAC 
ATCCTGAGCG CACAGCTGCC GCACATGCAG CCCTACATCC GCTTCTGCAG CCGCCAGCTC 
AACGGGGCTG CCCTGATCCA GCAGAAGACG GATGAGGCCC CAGACTTCAA GGAGTTCGTC 
AAAAGATTGG CAATGGATCC TCGGTGTAAA GGGATGCCAC TCTCTAGTTT TATACTGAAG 
CCTATGCAAC GGGTAACAAG ATACCCACTG ATCATTAAAA ATATCCTGGA AAACACCCCT 
GAAAACCACC CGGACCACAG CCACTTGAAG CACGCCCTGG AGAAGGCGGA AGAGCTCTGT 
TCCCAGGTGA ACGAAGGGGT GCGGGAGAAG GAGAACTCTG ACCGGCTGGA GTGGATCCAG 
GCCCACGTGC AGTGTGAAGG CCTGTCTGAG CAACTTGTGT TCAATTCAGT GACCAATTGC 
TTGGGGCCGC GCAAATTTCT GCACAGTGGG AAGCTCTACA AGGCCAAGAG CAACAAGGAG 
CTGTATGGCT TCCTTTTCAA CGACTTCCTC CTGCTGACTC AGATCACGAA GCCTTTGGGG 
TCTTCTGGCA CCGACAAAGT CTTCAGCCCC AAATCAAACC TGCAGTATAA AATGTATAAA 
ACACCTATTT TCCTAAATGA GGTTCTAGTA AAATTACCCA CCGACCCTTC TGGAGACGAG 
CCCATCTTCC ACATCTCCCA CATTGACCGC GTCTATACTC TCCGAGCAGA AAGCATAAAT 
GAAAGGACTG CCTGGGTGCA GAAAATCAAA GCTGCTTCTG AACTCTACAT AGAGACTGAG 
AAAAAGAAGC GCGAGAAAGC GTACCTGGTC CGTTCCCAAA GGGCAACAGG CATTGGAAGG 
TTGATGGTGA ACGTGGTTGA AGGCATCGAG TTGAAACCCT GTCGGTCACA TGGAAAGAGC 
AACCCGTACT GTGAGGTGAC CATGGGTTCC CAGTGCCACA TCACCAAGAC GATCCAGGAC 
ACTCTGAACC CCAAGTGGAA TTCCAACTGC CAGTTCTTCA TCCGAGACCT GGAGCAGGAA 
GTCCTCTGCA TCACTGTGTT CGAGAGGGAC CAGTTCTCAC CAGATGATTT TTTGGGTCGG 
ACGGAGATCC GTGTGGCGGA CATCAAGAAA GACCAGGGCT CCAAAGGTCC AGTTACGAAG 
TGTCTTCTGC TGCACGAAGT CCCCACGGGA GAGATTGTGG TCCGCTTGGA CCTGCAGTTG 
TTTGATGAGC CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883918.1
CDS	347..5401
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028429.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028429.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAATTT ATTGCCATGT ACACTTACGA GAGTTCTGAG 
CAAGGAGATT TAACCTTTCA GCAAGGGGAT GTGATTTTGG TTACCAAGAA AGATGGTGAC 
TGGTGGACAG GAACAGTGGG CGACAAGGCC GGAGTCTTCC CTTCTAACTA TGTGAGGCTT 
AAAGATTCAG AGGGCTCTGG AACTGCTGGG AAAACAGGGA GTTTAGGAAA AAAACCTGAA 
ATTGCCCAGG TTATTGCCTC ATACACCGCC ACCGGCCCCG AGCAGCTCAC TCTCGCCCCT 
GGTCAGCTGA TTTTGATCCG AAAAAAGAAC CCAGGTGGAT GGTGGGAAGG AGAGCTGCAA 
GCACGTGGGA AAAAGCGCCA GATAGGCTGG TTCCCAGCTA ATTATGTAAA GCTTCTAAGC 
CCTGGGACGA GCAAAATCAC TCCAACAGAG CCACCTAAGT CAACAGCATT AGCGGCAGTG 
TGCCAGGTGA TTGGGATGTA CGACTACACC GCGCAGAATG ACGATGAGCT GGCCTTCAAC 
AAGGGCCAGA TCATCAACGT CCTCAACAAG GAGGACCCTG ACTGGTGGAA AGGAGAAGTC 
AATGGACAAG TGGGGCTCTT CCCATCCAAT TATGTGAAGC TGACCACAGA CATGGACCCA 
AGCCAGCAAT GGTGTTCAGA CTTACATCTC TTGGATATGT TGACCCCAAC TGAAAGAAAG 
CGACAAGGAT ACATCCACGA GCTCATTGTC ACCGAGGAGA ACTATGTGAA TGACCTGCAG 
CTGGTCACAG AGATTTTTCA AAAACCCCTG ATGGAGTCTG AGCTGCTGAC AGAAAAAGAG 
GTTGCTATGA TTTTTGTGAA CTGGAAGGAG CTGATTATGT GTAATATCAA ACTACTAAAA 
GCGCTGAGAG TCCGCAAGAA GATGTCCGGG GAGAAGATGC CTGTGAAGAT GATTGGAGAC 
ATCCTGAGCG CACAGCTGCC GCACATGCAG CCCTACATCC GCTTCTGCAG CCGCCAGCTC 
AACGGGGCTG CCCTGATCCA GCAGAAGACG GATGAGGCCC CAGACTTCAA GGAGTTCGTC 
AAAAGATTGG CAATGGATCC TCGGTGTAAA GGGATGCCAC TCTCTAGTTT TATACTGAAG 
CCTATGCAAC GGGTAACAAG ATACCCACTG ATCATTAAAA ATATCCTGGA AAACACCCCT 
GAAAACCACC CGGACCACAG CCACTTGAAG CACGCCCTGG AGAAGGCGGA AGAGCTCTGT 
TCCCAGGTGA ACGAAGGGGT GCGGGAGAAG GAGAACTCTG ACCGGCTGGA GTGGATCCAG 
GCCCACGTGC AGTGTGAAGG CCTGTCTGAG CAACTTGTGT TCAATTCAGT GACCAATTGC 
TTGGGGCCGC GCAAATTTCT GCACAGTGGG AAGCTCTACA AGGCCAAGAG CAACAAGGAG 
CTGTATGGCT TCCTTTTCAA CGACTTCCTC CTGCTGACTC AGATCACGAA GCCTTTGGGG 
TCTTCTGGCA CCGACAAAGT CTTCAGCCCC AAATCAAACC TGCAGTATAA AATGTATAAA 
ACACCTATTT TCCTAAATGA GGTTCTAGTA AAATTACCCA CCGACCCTTC TGGAGACGAG 
CCCATCTTCC ACATCTCCCA CATTGACCGC GTCTATACTC TCCGAGCAGA AAGCATAAAT 
GAAAGGACTG CCTGGGTGCA GAAAATCAAA GCTGCTTCTG AACTCTACAT AGAGACTGAG 
AAAAAGAAGC GCGAGAAAGC GTACCTGGTC CGTTCCCAAA GGGCAACAGG CATTGGAAGG 
TTGATGGTGA ACGTGGTTGA AGGCATCGAG TTGAAACCCT GTCGGTCACA TGGAAAGAGC 
AACCCGTACT GTGAGGTGAC CATGGGTTCC CAGTGCCACA TCACCAAGAC GATCCAGGAC 
ACTCTGAACC CCAAGTGGAA TTCCAACTGC CAGTTCTTCA TCCGAGACCT GGAGCAGGAA 
GTCCTCTGCA TCACTGTGTT CGAGAGGGAC CAGTTCTCAC CAGATGATTT TTTGGGTCGG 
ACGGAGATCC GTGTGGCGGA CATCAAGAAA GACCAGGGCT CCAAAGGTCC AGTTACGAAG 
TGTCTTCTGC TGCACGAAGT CCCCACGGGA GAGATTGTGG TCCGCTTGGA CCTGCAGTTG 
TTTGATGAGC CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028429.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883918.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X2, mRNA.

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CloneID	OHu93293
Clone ID Related Accession (Same CDS sequence)	XM_017028429.1 , XM_017028429.2
Accession Version	XM_017028429.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028429.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAATTT ATTGCCATGT ACACTTACGA GAGTTCTGAG 
CAAGGAGATT TAACCTTTCA GCAAGGGGAT GTGATTTTGG TTACCAAGAA AGATGGTGAC 
TGGTGGACAG GAACAGTGGG CGACAAGGCC GGAGTCTTCC CTTCTAACTA TGTGAGGCTT 
AAAGATTCAG AGGGCTCTGG AACTGCTGGG AAAACAGGGA GTTTAGGAAA AAAACCTGAA 
ATTGCCCAGG TTATTGCCTC ATACACCGCC ACCGGCCCCG AGCAGCTCAC TCTCGCCCCT 
GGTCAGCTGA TTTTGATCCG AAAAAAGAAC CCAGGTGGAT GGTGGGAAGG AGAGCTGCAA 
GCACGTGGGA AAAAGCGCCA GATAGGCTGG TTCCCAGCTA ATTATGTAAA GCTTCTAAGC 
CCTGGGACGA GCAAAATCAC TCCAACAGAG CCACCTAAGT CAACAGCATT AGCGGCAGTG 
TGCCAGGTGA TTGGGATGTA CGACTACACC GCGCAGAATG ACGATGAGCT GGCCTTCAAC 
AAGGGCCAGA TCATCAACGT CCTCAACAAG GAGGACCCTG ACTGGTGGAA AGGAGAAGTC 
AATGGACAAG TGGGGCTCTT CCCATCCAAT TATGTGAAGC TGACCACAGA CATGGACCCA 
AGCCAGCAAT GGTGTTCAGA CTTACATCTC TTGGATATGT TGACCCCAAC TGAAAGAAAG 
CGACAAGGAT ACATCCACGA GCTCATTGTC ACCGAGGAGA ACTATGTGAA TGACCTGCAG 
CTGGTCACAG AGATTTTTCA AAAACCCCTG ATGGAGTCTG AGCTGCTGAC AGAAAAAGAG 
GTTGCTATGA TTTTTGTGAA CTGGAAGGAG CTGATTATGT GTAATATCAA ACTACTAAAA 
GCGCTGAGAG TCCGCAAGAA GATGTCCGGG GAGAAGATGC CTGTGAAGAT GATTGGAGAC 
ATCCTGAGCG CACAGCTGCC GCACATGCAG CCCTACATCC GCTTCTGCAG CCGCCAGCTC 
AACGGGGCTG CCCTGATCCA GCAGAAGACG GATGAGGCCC CAGACTTCAA GGAGTTCGTC 
AAAAGATTGG CAATGGATCC TCGGTGTAAA GGGATGCCAC TCTCTAGTTT TATACTGAAG 
CCTATGCAAC GGGTAACAAG ATACCCACTG ATCATTAAAA ATATCCTGGA AAACACCCCT 
GAAAACCACC CGGACCACAG CCACTTGAAG CACGCCCTGG AGAAGGCGGA AGAGCTCTGT 
TCCCAGGTGA ACGAAGGGGT GCGGGAGAAG GAGAACTCTG ACCGGCTGGA GTGGATCCAG 
GCCCACGTGC AGTGTGAAGG CCTGTCTGAG CAACTTGTGT TCAATTCAGT GACCAATTGC 
TTGGGGCCGC GCAAATTTCT GCACAGTGGG AAGCTCTACA AGGCCAAGAG CAACAAGGAG 
CTGTATGGCT TCCTTTTCAA CGACTTCCTC CTGCTGACTC AGATCACGAA GCCTTTGGGG 
TCTTCTGGCA CCGACAAAGT CTTCAGCCCC AAATCAAACC TGCAGTATAA AATGTATAAA 
ACACCTATTT TCCTAAATGA GGTTCTAGTA AAATTACCCA CCGACCCTTC TGGAGACGAG 
CCCATCTTCC ACATCTCCCA CATTGACCGC GTCTATACTC TCCGAGCAGA AAGCATAAAT 
GAAAGGACTG CCTGGGTGCA GAAAATCAAA GCTGCTTCTG AACTCTACAT AGAGACTGAG 
AAAAAGAAGC GCGAGAAAGC GTACCTGGTC CGTTCCCAAA GGGCAACAGG CATTGGAAGG 
TTGATGGTGA ACGTGGTTGA AGGCATCGAG TTGAAACCCT GTCGGTCACA TGGAAAGAGC 
AACCCGTACT GTGAGGTGAC CATGGGTTCC CAGTGCCACA TCACCAAGAC GATCCAGGAC 
ACTCTGAACC CCAAGTGGAA TTCCAACTGC CAGTTCTTCA TCCGAGACCT GGAGCAGGAA 
GTCCTCTGCA TCACTGTGTT CGAGAGGGAC CAGTTCTCAC CAGATGATTT TTTGGGTCGG 
ACGGAGATCC GTGTGGCGGA CATCAAGAAA GACCAGGGCT CCAAAGGTCC AGTTACGAAG 
TGTCTTCTGC TGCACGAAGT CCCCACGGGA GAGATTGTGG TCCGCTTGGA CCTGCAGTTG 
TTTGATGAGC CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883918.1
CDS	295..5349
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028429.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028429.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAATTT ATTGCCATGT ACACTTACGA GAGTTCTGAG 
CAAGGAGATT TAACCTTTCA GCAAGGGGAT GTGATTTTGG TTACCAAGAA AGATGGTGAC 
TGGTGGACAG GAACAGTGGG CGACAAGGCC GGAGTCTTCC CTTCTAACTA TGTGAGGCTT 
AAAGATTCAG AGGGCTCTGG AACTGCTGGG AAAACAGGGA GTTTAGGAAA AAAACCTGAA 
ATTGCCCAGG TTATTGCCTC ATACACCGCC ACCGGCCCCG AGCAGCTCAC TCTCGCCCCT 
GGTCAGCTGA TTTTGATCCG AAAAAAGAAC CCAGGTGGAT GGTGGGAAGG AGAGCTGCAA 
GCACGTGGGA AAAAGCGCCA GATAGGCTGG TTCCCAGCTA ATTATGTAAA GCTTCTAAGC 
CCTGGGACGA GCAAAATCAC TCCAACAGAG CCACCTAAGT CAACAGCATT AGCGGCAGTG 
TGCCAGGTGA TTGGGATGTA CGACTACACC GCGCAGAATG ACGATGAGCT GGCCTTCAAC 
AAGGGCCAGA TCATCAACGT CCTCAACAAG GAGGACCCTG ACTGGTGGAA AGGAGAAGTC 
AATGGACAAG TGGGGCTCTT CCCATCCAAT TATGTGAAGC TGACCACAGA CATGGACCCA 
AGCCAGCAAT GGTGTTCAGA CTTACATCTC TTGGATATGT TGACCCCAAC TGAAAGAAAG 
CGACAAGGAT ACATCCACGA GCTCATTGTC ACCGAGGAGA ACTATGTGAA TGACCTGCAG 
CTGGTCACAG AGATTTTTCA AAAACCCCTG ATGGAGTCTG AGCTGCTGAC AGAAAAAGAG 
GTTGCTATGA TTTTTGTGAA CTGGAAGGAG CTGATTATGT GTAATATCAA ACTACTAAAA 
GCGCTGAGAG TCCGCAAGAA GATGTCCGGG GAGAAGATGC CTGTGAAGAT GATTGGAGAC 
ATCCTGAGCG CACAGCTGCC GCACATGCAG CCCTACATCC GCTTCTGCAG CCGCCAGCTC 
AACGGGGCTG CCCTGATCCA GCAGAAGACG GATGAGGCCC CAGACTTCAA GGAGTTCGTC 
AAAAGATTGG CAATGGATCC TCGGTGTAAA GGGATGCCAC TCTCTAGTTT TATACTGAAG 
CCTATGCAAC GGGTAACAAG ATACCCACTG ATCATTAAAA ATATCCTGGA AAACACCCCT 
GAAAACCACC CGGACCACAG CCACTTGAAG CACGCCCTGG AGAAGGCGGA AGAGCTCTGT 
TCCCAGGTGA ACGAAGGGGT GCGGGAGAAG GAGAACTCTG ACCGGCTGGA GTGGATCCAG 
GCCCACGTGC AGTGTGAAGG CCTGTCTGAG CAACTTGTGT TCAATTCAGT GACCAATTGC 
TTGGGGCCGC GCAAATTTCT GCACAGTGGG AAGCTCTACA AGGCCAAGAG CAACAAGGAG 
CTGTATGGCT TCCTTTTCAA CGACTTCCTC CTGCTGACTC AGATCACGAA GCCTTTGGGG 
TCTTCTGGCA CCGACAAAGT CTTCAGCCCC AAATCAAACC TGCAGTATAA AATGTATAAA 
ACACCTATTT TCCTAAATGA GGTTCTAGTA AAATTACCCA CCGACCCTTC TGGAGACGAG 
CCCATCTTCC ACATCTCCCA CATTGACCGC GTCTATACTC TCCGAGCAGA AAGCATAAAT 
GAAAGGACTG CCTGGGTGCA GAAAATCAAA GCTGCTTCTG AACTCTACAT AGAGACTGAG 
AAAAAGAAGC GCGAGAAAGC GTACCTGGTC CGTTCCCAAA GGGCAACAGG CATTGGAAGG 
TTGATGGTGA ACGTGGTTGA AGGCATCGAG TTGAAACCCT GTCGGTCACA TGGAAAGAGC 
AACCCGTACT GTGAGGTGAC CATGGGTTCC CAGTGCCACA TCACCAAGAC GATCCAGGAC 
ACTCTGAACC CCAAGTGGAA TTCCAACTGC CAGTTCTTCA TCCGAGACCT GGAGCAGGAA 
GTCCTCTGCA TCACTGTGTT CGAGAGGGAC CAGTTCTCAC CAGATGATTT TTTGGGTCGG 
ACGGAGATCC GTGTGGCGGA CATCAAGAAA GACCAGGGCT CCAAAGGTCC AGTTACGAAG 
TGTCTTCTGC TGCACGAAGT CCCCACGGGA GAGATTGTGG TCCGCTTGGA CCTGCAGTTG 
TTTGATGAGC CGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028430.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883919.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu93294
Clone ID Related Accession (Same CDS sequence)	XM_017028430.2 , XM_017028430.1
Accession Version	XM_017028430.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028430.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AATTTATTGC CATGTACACT TACGAGAGTT CTGAGCAAGG AGATTTAACC 
TTTCAGCAAG GGGATGTGAT TTTGGTTACC AAGAAAGATG GTGACTGGTG GACAGGAACA 
GTGGGCGACA AGGCCGGAGT CTTCCCTTCT AACTATGTGA GGCTTAAAGA TTCAGAGGGC 
TCTGGAACTG CTGGGAAAAC AGGGAGTTTA GGAAAAAAAC CTGAAATTGC CCAGGTTATT 
GCCTCATACA CCGCCACCGG CCCCGAGCAG CTCACTCTCG CCCCTGGTCA GCTGATTTTG 
ATCCGAAAAA AGAACCCAGG TGGATGGTGG GAAGGAGAGC TGCAAGCACG TGGGAAAAAG 
CGCCAGATAG GCTGGTTCCC AGCTAATTAT GTAAAGCTTC TAAGCCCTGG GACGAGCAAA 
ATCACTCCAA CAGAGCCACC TAAGTCAACA GCATTAGCGG CAGTGTGCCA GGTGATTGGG 
ATGTACGACT ACACCGCGCA GAATGACGAT GAGCTGGCCT TCAACAAGGG CCAGATCATC 
AACGTCCTCA ACAAGGAGGA CCCTGACTGG TGGAAAGGAG AAGTCAATGG ACAAGTGGGG 
CTCTTCCCAT CCAATTATGT GAAGCTGACC ACAGACATGG ACCCAAGCCA GCAATGGTGT 
TCAGACTTAC ATCTCTTGGA TATGTTGACC CCAACTGAAA GAAAGCGACA AGGATACATC 
CACGAGCTCA TTGTCACCGA GGAGAACTAT GTGAATGACC TGCAGCTGGT CACAGAGATT 
TTTCAAAAAC CCCTGATGGA GTCTGAGCTG CTGACAGAAA AAGAGGTTGC TATGATTTTT 
GTGAACTGGA AGGAGCTGAT TATGTGTAAT ATCAAACTAC TAAAAGCGCT GAGAGTCCGC 
AAGAAGATGT CCGGGGAGAA GATGCCTGTG AAGATGATTG GAGACATCCT GAGCGCACAG 
CTGCCGCACA TGCAGCCCTA CATCCGCTTC TGCAGCCGCC AGCTCAACGG GGCTGCCCTG 
ATCCAGCAGA AGACGGATGA GGCCCCAGAC TTCAAGGAGT TCGTCAAAAG ATTGGCAATG 
GATCCTCGGT GTAAAGGGAT GCCACTCTCT AGTTTTATAC TGAAGCCTAT GCAACGGGTA 
ACAAGATACC CACTGATCAT TAAAAATATC CTGGAAAACA CCCCTGAAAA CCACCCGGAC 
CACAGCCACT TGAAGCACGC CCTGGAGAAG GCGGAAGAGC TCTGTTCCCA GGTGAACGAA 
GGGGTGCGGG AGAAGGAGAA CTCTGACCGG CTGGAGTGGA TCCAGGCCCA CGTGCAGTGT 
GAAGGCCTGT CTGAGCAACT TGTGTTCAAT TCAGTGACCA ATTGCTTGGG GCCGCGCAAA 
TTTCTGCACA GTGGGAAGCT CTACAAGGCC AAGAGCAACA AGGAGCTGTA TGGCTTCCTT 
TTCAACGACT TCCTCCTGCT GACTCAGATC ACGAAGCCTT TGGGGTCTTC TGGCACCGAC 
AAAGTCTTCA GCCCCAAATC AAACCTGCAG TATAAAATGT ATAAAACACC TATTTTCCTA 
AATGAGGTTC TAGTAAAATT ACCCACCGAC CCTTCTGGAG ACGAGCCCAT CTTCCACATC 
TCCCACATTG ACCGCGTCTA TACTCTCCGA GCAGAAAGCA TAAATGAAAG GACTGCCTGG 
GTGCAGAAAA TCAAAGCTGC TTCTGAACTC TACATAGAGA CTGAGAAAAA GAAGCGCGAG 
AAAGCGTACC TGGTCCGTTC CCAAAGGGCA ACAGGCATTG GAAGGTTGAT GGTGAACGTG 
GTTGAAGGCA TCGAGTTGAA ACCCTGTCGG TCACATGGAA AGAGCAACCC GTACTGTGAG 
GTGACCATGG GTTCCCAGTG CCACATCACC AAGACGATCC AGGACACTCT GAACCCCAAG 
TGGAATTCCA ACTGCCAGTT CTTCATCCGA GACCTGGAGC AGGAAGTCCT CTGCATCACT 
GTGTTCGAGA GGGACCAGTT CTCACCAGAT GATTTTTTGG GTCGGACGGA GATCCGTGTG 
GCGGACATCA AGAAAGACCA GGGCTCCAAA GGTCCAGTTA CGAAGTGTCT TCTGCTGCAC 
GAAGTCCCCA CGGGAGAGAT TGTGGTCCGC TTGGACCTGC AGTTGTTTGA TGAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883919.1
CDS	295..5334
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028430.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028430.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AATTTATTGC CATGTACACT TACGAGAGTT CTGAGCAAGG AGATTTAACC 
TTTCAGCAAG GGGATGTGAT TTTGGTTACC AAGAAAGATG GTGACTGGTG GACAGGAACA 
GTGGGCGACA AGGCCGGAGT CTTCCCTTCT AACTATGTGA GGCTTAAAGA TTCAGAGGGC 
TCTGGAACTG CTGGGAAAAC AGGGAGTTTA GGAAAAAAAC CTGAAATTGC CCAGGTTATT 
GCCTCATACA CCGCCACCGG CCCCGAGCAG CTCACTCTCG CCCCTGGTCA GCTGATTTTG 
ATCCGAAAAA AGAACCCAGG TGGATGGTGG GAAGGAGAGC TGCAAGCACG TGGGAAAAAG 
CGCCAGATAG GCTGGTTCCC AGCTAATTAT GTAAAGCTTC TAAGCCCTGG GACGAGCAAA 
ATCACTCCAA CAGAGCCACC TAAGTCAACA GCATTAGCGG CAGTGTGCCA GGTGATTGGG 
ATGTACGACT ACACCGCGCA GAATGACGAT GAGCTGGCCT TCAACAAGGG CCAGATCATC 
AACGTCCTCA ACAAGGAGGA CCCTGACTGG TGGAAAGGAG AAGTCAATGG ACAAGTGGGG 
CTCTTCCCAT CCAATTATGT GAAGCTGACC ACAGACATGG ACCCAAGCCA GCAATGGTGT 
TCAGACTTAC ATCTCTTGGA TATGTTGACC CCAACTGAAA GAAAGCGACA AGGATACATC 
CACGAGCTCA TTGTCACCGA GGAGAACTAT GTGAATGACC TGCAGCTGGT CACAGAGATT 
TTTCAAAAAC CCCTGATGGA GTCTGAGCTG CTGACAGAAA AAGAGGTTGC TATGATTTTT 
GTGAACTGGA AGGAGCTGAT TATGTGTAAT ATCAAACTAC TAAAAGCGCT GAGAGTCCGC 
AAGAAGATGT CCGGGGAGAA GATGCCTGTG AAGATGATTG GAGACATCCT GAGCGCACAG 
CTGCCGCACA TGCAGCCCTA CATCCGCTTC TGCAGCCGCC AGCTCAACGG GGCTGCCCTG 
ATCCAGCAGA AGACGGATGA GGCCCCAGAC TTCAAGGAGT TCGTCAAAAG ATTGGCAATG 
GATCCTCGGT GTAAAGGGAT GCCACTCTCT AGTTTTATAC TGAAGCCTAT GCAACGGGTA 
ACAAGATACC CACTGATCAT TAAAAATATC CTGGAAAACA CCCCTGAAAA CCACCCGGAC 
CACAGCCACT TGAAGCACGC CCTGGAGAAG GCGGAAGAGC TCTGTTCCCA GGTGAACGAA 
GGGGTGCGGG AGAAGGAGAA CTCTGACCGG CTGGAGTGGA TCCAGGCCCA CGTGCAGTGT 
GAAGGCCTGT CTGAGCAACT TGTGTTCAAT TCAGTGACCA ATTGCTTGGG GCCGCGCAAA 
TTTCTGCACA GTGGGAAGCT CTACAAGGCC AAGAGCAACA AGGAGCTGTA TGGCTTCCTT 
TTCAACGACT TCCTCCTGCT GACTCAGATC ACGAAGCCTT TGGGGTCTTC TGGCACCGAC 
AAAGTCTTCA GCCCCAAATC AAACCTGCAG TATAAAATGT ATAAAACACC TATTTTCCTA 
AATGAGGTTC TAGTAAAATT ACCCACCGAC CCTTCTGGAG ACGAGCCCAT CTTCCACATC 
TCCCACATTG ACCGCGTCTA TACTCTCCGA GCAGAAAGCA TAAATGAAAG GACTGCCTGG 
GTGCAGAAAA TCAAAGCTGC TTCTGAACTC TACATAGAGA CTGAGAAAAA GAAGCGCGAG 
AAAGCGTACC TGGTCCGTTC CCAAAGGGCA ACAGGCATTG GAAGGTTGAT GGTGAACGTG 
GTTGAAGGCA TCGAGTTGAA ACCCTGTCGG TCACATGGAA AGAGCAACCC GTACTGTGAG 
GTGACCATGG GTTCCCAGTG CCACATCACC AAGACGATCC AGGACACTCT GAACCCCAAG 
TGGAATTCCA ACTGCCAGTT CTTCATCCGA GACCTGGAGC AGGAAGTCCT CTGCATCACT 
GTGTTCGAGA GGGACCAGTT CTCACCAGAT GATTTTTTGG GTCGGACGGA GATCCGTGTG 
GCGGACATCA AGAAAGACCA GGGCTCCAAA GGTCCAGTTA CGAAGTGTCT TCTGCTGCAC 
GAAGTCCCCA CGGGAGAGAT TGTGGTCCGC TTGGACCTGC AGTTGTTTGA TGAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028430.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883919.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X4, mRNA.

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CloneID	OHu93294
Clone ID Related Accession (Same CDS sequence)	XM_017028430.2 , XM_017028430.1
Accession Version	XM_017028430.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5040bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AATTTATTGC CATGTACACT TACGAGAGTT CTGAGCAAGG AGATTTAACC 
TTTCAGCAAG GGGATGTGAT TTTGGTTACC AAGAAAGATG GTGACTGGTG GACAGGAACA 
GTGGGCGACA AGGCCGGAGT CTTCCCTTCT AACTATGTGA GGCTTAAAGA TTCAGAGGGC 
TCTGGAACTG CTGGGAAAAC AGGGAGTTTA GGAAAAAAAC CTGAAATTGC CCAGGTTATT 
GCCTCATACA CCGCCACCGG CCCCGAGCAG CTCACTCTCG CCCCTGGTCA GCTGATTTTG 
ATCCGAAAAA AGAACCCAGG TGGATGGTGG GAAGGAGAGC TGCAAGCACG TGGGAAAAAG 
CGCCAGATAG GCTGGTTCCC AGCTAATTAT GTAAAGCTTC TAAGCCCTGG GACGAGCAAA 
ATCACTCCAA CAGAGCCACC TAAGTCAACA GCATTAGCGG CAGTGTGCCA GGTGATTGGG 
ATGTACGACT ACACCGCGCA GAATGACGAT GAGCTGGCCT TCAACAAGGG CCAGATCATC 
AACGTCCTCA ACAAGGAGGA CCCTGACTGG TGGAAAGGAG AAGTCAATGG ACAAGTGGGG 
CTCTTCCCAT CCAATTATGT GAAGCTGACC ACAGACATGG ACCCAAGCCA GCAATGGTGT 
TCAGACTTAC ATCTCTTGGA TATGTTGACC CCAACTGAAA GAAAGCGACA AGGATACATC 
CACGAGCTCA TTGTCACCGA GGAGAACTAT GTGAATGACC TGCAGCTGGT CACAGAGATT 
TTTCAAAAAC CCCTGATGGA GTCTGAGCTG CTGACAGAAA AAGAGGTTGC TATGATTTTT 
GTGAACTGGA AGGAGCTGAT TATGTGTAAT ATCAAACTAC TAAAAGCGCT GAGAGTCCGC 
AAGAAGATGT CCGGGGAGAA GATGCCTGTG AAGATGATTG GAGACATCCT GAGCGCACAG 
CTGCCGCACA TGCAGCCCTA CATCCGCTTC TGCAGCCGCC AGCTCAACGG GGCTGCCCTG 
ATCCAGCAGA AGACGGATGA GGCCCCAGAC TTCAAGGAGT TCGTCAAAAG ATTGGCAATG 
GATCCTCGGT GTAAAGGGAT GCCACTCTCT AGTTTTATAC TGAAGCCTAT GCAACGGGTA 
ACAAGATACC CACTGATCAT TAAAAATATC CTGGAAAACA CCCCTGAAAA CCACCCGGAC 
CACAGCCACT TGAAGCACGC CCTGGAGAAG GCGGAAGAGC TCTGTTCCCA GGTGAACGAA 
GGGGTGCGGG AGAAGGAGAA CTCTGACCGG CTGGAGTGGA TCCAGGCCCA CGTGCAGTGT 
GAAGGCCTGT CTGAGCAACT TGTGTTCAAT TCAGTGACCA ATTGCTTGGG GCCGCGCAAA 
TTTCTGCACA GTGGGAAGCT CTACAAGGCC AAGAGCAACA AGGAGCTGTA TGGCTTCCTT 
TTCAACGACT TCCTCCTGCT GACTCAGATC ACGAAGCCTT TGGGGTCTTC TGGCACCGAC 
AAAGTCTTCA GCCCCAAATC AAACCTGCAG TATAAAATGT ATAAAACACC TATTTTCCTA 
AATGAGGTTC TAGTAAAATT ACCCACCGAC CCTTCTGGAG ACGAGCCCAT CTTCCACATC 
TCCCACATTG ACCGCGTCTA TACTCTCCGA GCAGAAAGCA TAAATGAAAG GACTGCCTGG 
GTGCAGAAAA TCAAAGCTGC TTCTGAACTC TACATAGAGA CTGAGAAAAA GAAGCGCGAG 
AAAGCGTACC TGGTCCGTTC CCAAAGGGCA ACAGGCATTG GAAGGTTGAT GGTGAACGTG 
GTTGAAGGCA TCGAGTTGAA ACCCTGTCGG TCACATGGAA AGAGCAACCC GTACTGTGAG 
GTGACCATGG GTTCCCAGTG CCACATCACC AAGACGATCC AGGACACTCT GAACCCCAAG 
TGGAATTCCA ACTGCCAGTT CTTCATCCGA GACCTGGAGC AGGAAGTCCT CTGCATCACT 
GTGTTCGAGA GGGACCAGTT CTCACCAGAT GATTTTTTGG GTCGGACGGA GATCCGTGTG 
GCGGACATCA AGAAAGACCA GGGCTCCAAA GGTCCAGTTA CGAAGTGTCT TCTGCTGCAC 
GAAGTCCCCA CGGGAGAGAT TGTGGTCCGC TTGGACCTGC AGTTGTTTGA TGAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883919.1
CDS	347..5386
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028430.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028430.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AATTTATTGC CATGTACACT TACGAGAGTT CTGAGCAAGG AGATTTAACC 
TTTCAGCAAG GGGATGTGAT TTTGGTTACC AAGAAAGATG GTGACTGGTG GACAGGAACA 
GTGGGCGACA AGGCCGGAGT CTTCCCTTCT AACTATGTGA GGCTTAAAGA TTCAGAGGGC 
TCTGGAACTG CTGGGAAAAC AGGGAGTTTA GGAAAAAAAC CTGAAATTGC CCAGGTTATT 
GCCTCATACA CCGCCACCGG CCCCGAGCAG CTCACTCTCG CCCCTGGTCA GCTGATTTTG 
ATCCGAAAAA AGAACCCAGG TGGATGGTGG GAAGGAGAGC TGCAAGCACG TGGGAAAAAG 
CGCCAGATAG GCTGGTTCCC AGCTAATTAT GTAAAGCTTC TAAGCCCTGG GACGAGCAAA 
ATCACTCCAA CAGAGCCACC TAAGTCAACA GCATTAGCGG CAGTGTGCCA GGTGATTGGG 
ATGTACGACT ACACCGCGCA GAATGACGAT GAGCTGGCCT TCAACAAGGG CCAGATCATC 
AACGTCCTCA ACAAGGAGGA CCCTGACTGG TGGAAAGGAG AAGTCAATGG ACAAGTGGGG 
CTCTTCCCAT CCAATTATGT GAAGCTGACC ACAGACATGG ACCCAAGCCA GCAATGGTGT 
TCAGACTTAC ATCTCTTGGA TATGTTGACC CCAACTGAAA GAAAGCGACA AGGATACATC 
CACGAGCTCA TTGTCACCGA GGAGAACTAT GTGAATGACC TGCAGCTGGT CACAGAGATT 
TTTCAAAAAC CCCTGATGGA GTCTGAGCTG CTGACAGAAA AAGAGGTTGC TATGATTTTT 
GTGAACTGGA AGGAGCTGAT TATGTGTAAT ATCAAACTAC TAAAAGCGCT GAGAGTCCGC 
AAGAAGATGT CCGGGGAGAA GATGCCTGTG AAGATGATTG GAGACATCCT GAGCGCACAG 
CTGCCGCACA TGCAGCCCTA CATCCGCTTC TGCAGCCGCC AGCTCAACGG GGCTGCCCTG 
ATCCAGCAGA AGACGGATGA GGCCCCAGAC TTCAAGGAGT TCGTCAAAAG ATTGGCAATG 
GATCCTCGGT GTAAAGGGAT GCCACTCTCT AGTTTTATAC TGAAGCCTAT GCAACGGGTA 
ACAAGATACC CACTGATCAT TAAAAATATC CTGGAAAACA CCCCTGAAAA CCACCCGGAC 
CACAGCCACT TGAAGCACGC CCTGGAGAAG GCGGAAGAGC TCTGTTCCCA GGTGAACGAA 
GGGGTGCGGG AGAAGGAGAA CTCTGACCGG CTGGAGTGGA TCCAGGCCCA CGTGCAGTGT 
GAAGGCCTGT CTGAGCAACT TGTGTTCAAT TCAGTGACCA ATTGCTTGGG GCCGCGCAAA 
TTTCTGCACA GTGGGAAGCT CTACAAGGCC AAGAGCAACA AGGAGCTGTA TGGCTTCCTT 
TTCAACGACT TCCTCCTGCT GACTCAGATC ACGAAGCCTT TGGGGTCTTC TGGCACCGAC 
AAAGTCTTCA GCCCCAAATC AAACCTGCAG TATAAAATGT ATAAAACACC TATTTTCCTA 
AATGAGGTTC TAGTAAAATT ACCCACCGAC CCTTCTGGAG ACGAGCCCAT CTTCCACATC 
TCCCACATTG ACCGCGTCTA TACTCTCCGA GCAGAAAGCA TAAATGAAAG GACTGCCTGG 
GTGCAGAAAA TCAAAGCTGC TTCTGAACTC TACATAGAGA CTGAGAAAAA GAAGCGCGAG 
AAAGCGTACC TGGTCCGTTC CCAAAGGGCA ACAGGCATTG GAAGGTTGAT GGTGAACGTG 
GTTGAAGGCA TCGAGTTGAA ACCCTGTCGG TCACATGGAA AGAGCAACCC GTACTGTGAG 
GTGACCATGG GTTCCCAGTG CCACATCACC AAGACGATCC AGGACACTCT GAACCCCAAG 
TGGAATTCCA ACTGCCAGTT CTTCATCCGA GACCTGGAGC AGGAAGTCCT CTGCATCACT 
GTGTTCGAGA GGGACCAGTT CTCACCAGAT GATTTTTTGG GTCGGACGGA GATCCGTGTG 
GCGGACATCA AGAAAGACCA GGGCTCCAAA GGTCCAGTTA CGAAGTGTCT TCTGCTGCAC 
GAAGTCCCCA CGGGAGAGAT TGTGGTCCGC TTGGACCTGC AGTTGTTTGA TGAGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028431.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883920.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X4, mRNA.

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CloneID	OHu93295
Clone ID Related Accession (Same CDS sequence)	XM_017028431.2 , XM_017028431.1
Accession Version	XM_017028431.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028431.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGGTGTTCAG ACTTACATCT CTTGGATATG TTGACCCCAA CTGAAAGAAA GCGACAAGGA 
TACATCCACG AGCTCATTGT CACCGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATTTTTC AAAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG 
ATTTTTGTGA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTAAA AGCGCTGAGA 
GTCCGCAAGA AGATGTCCGG GGAGAAGATG CCTGTGAAGA TGATTGGAGA CATCCTGAGC 
GCACAGCTGC CGCACATGCA GCCCTACATC CGCTTCTGCA GCCGCCAGCT CAACGGGGCT 
GCCCTGATCC AGCAGAAGAC GGATGAGGCC CCAGACTTCA AGGAGTTCGT CAAAAGATTG 
GCAATGGATC CTCGGTGTAA AGGGATGCCA CTCTCTAGTT TTATACTGAA GCCTATGCAA 
CGGGTAACAA GATACCCACT GATCATTAAA AATCAACTTG TGTTCAATTC AGTGACCAAT 
TGCTTGGGGC CGCGCAAATT TCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTTTT CAACGACTTC CTCCTGCTGA CTCAGATCAC GAAGCCTTTG 
GGGTCTTCTG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAT 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTAAAATTAC CCACCGACCC TTCTGGAGAC 
GAGCCCATCT TCCACATCTC CCACATTGAC CGCGTCTATA CTCTCCGAGC AGAAAGCATA 
AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCTGCTT CTGAACTCTA CATAGAGACT 
GAGAAAAAGA AGCGCGAGAA AGCGTACCTG GTCCGTTCCC AAAGGGCAAC AGGCATTGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATC GAGTTGAAAC CCTGTCGGTC ACATGGAAAG 
AGCAACCCGT ACTGTGAGGT GACCATGGGT TCCCAGTGCC ACATCACCAA GACGATCCAG 
GACACTCTGA ACCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCCGAGA CCTGGAGCAG 
GAAGTCCTCT GCATCACTGT GTTCGAGAGG GACCAGTTCT CACCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGTGTGGC GGACATCAAG AAAGACCAGG GCTCCAAAGG TCCAGTTACG 
AAGTGTCTTC TGCTGCACGA AGTCCCCACG GGAGAGATTG TGGTCCGCTT GGACCTGCAG 
TTGTTTGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883920.1
CDS	294..5291
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028431.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028431.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGGTGTTCAG ACTTACATCT CTTGGATATG TTGACCCCAA CTGAAAGAAA GCGACAAGGA 
TACATCCACG AGCTCATTGT CACCGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATTTTTC AAAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG 
ATTTTTGTGA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTAAA AGCGCTGAGA 
GTCCGCAAGA AGATGTCCGG GGAGAAGATG CCTGTGAAGA TGATTGGAGA CATCCTGAGC 
GCACAGCTGC CGCACATGCA GCCCTACATC CGCTTCTGCA GCCGCCAGCT CAACGGGGCT 
GCCCTGATCC AGCAGAAGAC GGATGAGGCC CCAGACTTCA AGGAGTTCGT CAAAAGATTG 
GCAATGGATC CTCGGTGTAA AGGGATGCCA CTCTCTAGTT TTATACTGAA GCCTATGCAA 
CGGGTAACAA GATACCCACT GATCATTAAA AATCAACTTG TGTTCAATTC AGTGACCAAT 
TGCTTGGGGC CGCGCAAATT TCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTTTT CAACGACTTC CTCCTGCTGA CTCAGATCAC GAAGCCTTTG 
GGGTCTTCTG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAT 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTAAAATTAC CCACCGACCC TTCTGGAGAC 
GAGCCCATCT TCCACATCTC CCACATTGAC CGCGTCTATA CTCTCCGAGC AGAAAGCATA 
AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCTGCTT CTGAACTCTA CATAGAGACT 
GAGAAAAAGA AGCGCGAGAA AGCGTACCTG GTCCGTTCCC AAAGGGCAAC AGGCATTGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATC GAGTTGAAAC CCTGTCGGTC ACATGGAAAG 
AGCAACCCGT ACTGTGAGGT GACCATGGGT TCCCAGTGCC ACATCACCAA GACGATCCAG 
GACACTCTGA ACCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCCGAGA CCTGGAGCAG 
GAAGTCCTCT GCATCACTGT GTTCGAGAGG GACCAGTTCT CACCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGTGTGGC GGACATCAAG AAAGACCAGG GCTCCAAAGG TCCAGTTACG 
AAGTGTCTTC TGCTGCACGA AGTCCCCACG GGAGAGATTG TGGTCCGCTT GGACCTGCAG 
TTGTTTGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028431.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883920.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X5, mRNA.

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CloneID	OHu93295
Clone ID Related Accession (Same CDS sequence)	XM_017028431.2 , XM_017028431.1
Accession Version	XM_017028431.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4998bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGGTGTTCAG ACTTACATCT CTTGGATATG TTGACCCCAA CTGAAAGAAA GCGACAAGGA 
TACATCCACG AGCTCATTGT CACCGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATTTTTC AAAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG 
ATTTTTGTGA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTAAA AGCGCTGAGA 
GTCCGCAAGA AGATGTCCGG GGAGAAGATG CCTGTGAAGA TGATTGGAGA CATCCTGAGC 
GCACAGCTGC CGCACATGCA GCCCTACATC CGCTTCTGCA GCCGCCAGCT CAACGGGGCT 
GCCCTGATCC AGCAGAAGAC GGATGAGGCC CCAGACTTCA AGGAGTTCGT CAAAAGATTG 
GCAATGGATC CTCGGTGTAA AGGGATGCCA CTCTCTAGTT TTATACTGAA GCCTATGCAA 
CGGGTAACAA GATACCCACT GATCATTAAA AATCAACTTG TGTTCAATTC AGTGACCAAT 
TGCTTGGGGC CGCGCAAATT TCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTTTT CAACGACTTC CTCCTGCTGA CTCAGATCAC GAAGCCTTTG 
GGGTCTTCTG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAT 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTAAAATTAC CCACCGACCC TTCTGGAGAC 
GAGCCCATCT TCCACATCTC CCACATTGAC CGCGTCTATA CTCTCCGAGC AGAAAGCATA 
AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCTGCTT CTGAACTCTA CATAGAGACT 
GAGAAAAAGA AGCGCGAGAA AGCGTACCTG GTCCGTTCCC AAAGGGCAAC AGGCATTGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATC GAGTTGAAAC CCTGTCGGTC ACATGGAAAG 
AGCAACCCGT ACTGTGAGGT GACCATGGGT TCCCAGTGCC ACATCACCAA GACGATCCAG 
GACACTCTGA ACCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCCGAGA CCTGGAGCAG 
GAAGTCCTCT GCATCACTGT GTTCGAGAGG GACCAGTTCT CACCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGTGTGGC GGACATCAAG AAAGACCAGG GCTCCAAAGG TCCAGTTACG 
AAGTGTCTTC TGCTGCACGA AGTCCCCACG GGAGAGATTG TGGTCCGCTT GGACCTGCAG 
TTGTTTGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883920.1
CDS	347..5344
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028431.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028431.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGGTGTTCAG ACTTACATCT CTTGGATATG TTGACCCCAA CTGAAAGAAA GCGACAAGGA 
TACATCCACG AGCTCATTGT CACCGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATTTTTC AAAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG 
ATTTTTGTGA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTAAA AGCGCTGAGA 
GTCCGCAAGA AGATGTCCGG GGAGAAGATG CCTGTGAAGA TGATTGGAGA CATCCTGAGC 
GCACAGCTGC CGCACATGCA GCCCTACATC CGCTTCTGCA GCCGCCAGCT CAACGGGGCT 
GCCCTGATCC AGCAGAAGAC GGATGAGGCC CCAGACTTCA AGGAGTTCGT CAAAAGATTG 
GCAATGGATC CTCGGTGTAA AGGGATGCCA CTCTCTAGTT TTATACTGAA GCCTATGCAA 
CGGGTAACAA GATACCCACT GATCATTAAA AATCAACTTG TGTTCAATTC AGTGACCAAT 
TGCTTGGGGC CGCGCAAATT TCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTTTT CAACGACTTC CTCCTGCTGA CTCAGATCAC GAAGCCTTTG 
GGGTCTTCTG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAT 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTAAAATTAC CCACCGACCC TTCTGGAGAC 
GAGCCCATCT TCCACATCTC CCACATTGAC CGCGTCTATA CTCTCCGAGC AGAAAGCATA 
AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCTGCTT CTGAACTCTA CATAGAGACT 
GAGAAAAAGA AGCGCGAGAA AGCGTACCTG GTCCGTTCCC AAAGGGCAAC AGGCATTGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATC GAGTTGAAAC CCTGTCGGTC ACATGGAAAG 
AGCAACCCGT ACTGTGAGGT GACCATGGGT TCCCAGTGCC ACATCACCAA GACGATCCAG 
GACACTCTGA ACCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCCGAGA CCTGGAGCAG 
GAAGTCCTCT GCATCACTGT GTTCGAGAGG GACCAGTTCT CACCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGTGTGGC GGACATCAAG AAAGACCAGG GCTCCAAAGG TCCAGTTACG 
AAGTGTCTTC TGCTGCACGA AGTCCCCACG GGAGAGATTG TGGTCCGCTT GGACCTGCAG 
TTGTTTGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028432.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883921.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X6, mRNA.

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CloneID	OHu93296
Clone ID Related Accession (Same CDS sequence)	XM_017028432.1 , XM_017028432.2
Accession Version	XM_017028432.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GAGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGGCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CCCAGGTTAT TGCCTCATAC 
ACCGCCACCG GCCCCGAGCA GCTCACTCTC GCCCCTGGTC AGCTGATTTT GATCCGAAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTGCAAGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTCC CAGCTAATTA TGTAAAGCTT CTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGAGCCAC CTAAGTCAAC AGCATTAGCG GCAGTGTGCC AGGTGATTGG GATGTACGAC 
TACACCGCGC AGAATGACGA TGAGCTGGCC TTCAACAAGG GCCAGATCAT CAACGTCCTC 
AACAAGGAGG ACCCTGACTG GTGGAAAGGA GAAGTCAATG GACAAGTGGG GCTCTTCCCA 
TCCAATTATG TGAAGCTGAC CACAGACATG GACCCAAGCC AGCAATGGTG TTCAGACTTA 
CATCTCTTGG ATATGTTGAC CCCAACTGAA AGAAAGCGAC AAGGATACAT CCACGAGCTC 
ATTGTCACCG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT TTTTCAAAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGTTG CTATGATTTT TGTGAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTAAAAGCGC TGAGAGTCCG CAAGAAGATG 
TCCGGGGAGA AGATGCCTGT GAAGATGATT GGAGACATCC TGAGCGCACA GCTGCCGCAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCCGC CAGCTCAACG GGGCTGCCCT GATCCAGCAG 
AAGACGGATG AGGCCCCAGA CTTCAAGGAG TTCGTCAAAA GATTGGCAAT GGATCCTCGG 
TGTAAAGGGA TGCCACTCTC TAGTTTTATA CTGAAGCCTA TGCAACGGGT AACAAGATAC 
CCACTGATCA TTAAAAATCA ACTTGTGTTC AATTCAGTGA CCAATTGCTT GGGGCCGCGC 
AAATTTCTGC ACAGTGGGAA GCTCTACAAG GCCAAGAGCA ACAAGGAGCT GTATGGCTTC 
CTTTTCAACG ACTTCCTCCT GCTGACTCAG ATCACGAAGC CTTTGGGGTC TTCTGGCACC 
GACAAAGTCT TCAGCCCCAA ATCAAACCTG CAGTATAAAA TGTATAAAAC ACCTATTTTC 
CTAAATGAGG TTCTAGTAAA ATTACCCACC GACCCTTCTG GAGACGAGCC CATCTTCCAC 
ATCTCCCACA TTGACCGCGT CTATACTCTC CGAGCAGAAA GCATAAATGA AAGGACTGCC 
TGGGTGCAGA AAATCAAAGC TGCTTCTGAA CTCTACATAG AGACTGAGAA AAAGAAGCGC 
GAGAAAGCGT ACCTGGTCCG TTCCCAAAGG GCAACAGGCA TTGGAAGGTT GATGGTGAAC 
GTGGTTGAAG GCATCGAGTT GAAACCCTGT CGGTCACATG GAAAGAGCAA CCCGTACTGT 
GAGGTGACCA TGGGTTCCCA GTGCCACATC ACCAAGACGA TCCAGGACAC TCTGAACCCC 
AAGTGGAATT CCAACTGCCA GTTCTTCATC CGAGACCTGG AGCAGGAAGT CCTCTGCATC 
ACTGTGTTCG AGAGGGACCA GTTCTCACCA GATGATTTTT TGGGTCGGAC GGAGATCCGT 
GTGGCGGACA TCAAGAAAGA CCAGGGCTCC AAAGGTCCAG TTACGAAGTG TCTTCTGCTG 
CACGAAGTCC CCACGGGAGA GATTGTGGTC CGCTTGGACC TGCAGTTGTT TGATGAGCCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883921.1
CDS	347..5329
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028432.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028432.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GAGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGGCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CCCAGGTTAT TGCCTCATAC 
ACCGCCACCG GCCCCGAGCA GCTCACTCTC GCCCCTGGTC AGCTGATTTT GATCCGAAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTGCAAGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTCC CAGCTAATTA TGTAAAGCTT CTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGAGCCAC CTAAGTCAAC AGCATTAGCG GCAGTGTGCC AGGTGATTGG GATGTACGAC 
TACACCGCGC AGAATGACGA TGAGCTGGCC TTCAACAAGG GCCAGATCAT CAACGTCCTC 
AACAAGGAGG ACCCTGACTG GTGGAAAGGA GAAGTCAATG GACAAGTGGG GCTCTTCCCA 
TCCAATTATG TGAAGCTGAC CACAGACATG GACCCAAGCC AGCAATGGTG TTCAGACTTA 
CATCTCTTGG ATATGTTGAC CCCAACTGAA AGAAAGCGAC AAGGATACAT CCACGAGCTC 
ATTGTCACCG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT TTTTCAAAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGTTG CTATGATTTT TGTGAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTAAAAGCGC TGAGAGTCCG CAAGAAGATG 
TCCGGGGAGA AGATGCCTGT GAAGATGATT GGAGACATCC TGAGCGCACA GCTGCCGCAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCCGC CAGCTCAACG GGGCTGCCCT GATCCAGCAG 
AAGACGGATG AGGCCCCAGA CTTCAAGGAG TTCGTCAAAA GATTGGCAAT GGATCCTCGG 
TGTAAAGGGA TGCCACTCTC TAGTTTTATA CTGAAGCCTA TGCAACGGGT AACAAGATAC 
CCACTGATCA TTAAAAATCA ACTTGTGTTC AATTCAGTGA CCAATTGCTT GGGGCCGCGC 
AAATTTCTGC ACAGTGGGAA GCTCTACAAG GCCAAGAGCA ACAAGGAGCT GTATGGCTTC 
CTTTTCAACG ACTTCCTCCT GCTGACTCAG ATCACGAAGC CTTTGGGGTC TTCTGGCACC 
GACAAAGTCT TCAGCCCCAA ATCAAACCTG CAGTATAAAA TGTATAAAAC ACCTATTTTC 
CTAAATGAGG TTCTAGTAAA ATTACCCACC GACCCTTCTG GAGACGAGCC CATCTTCCAC 
ATCTCCCACA TTGACCGCGT CTATACTCTC CGAGCAGAAA GCATAAATGA AAGGACTGCC 
TGGGTGCAGA AAATCAAAGC TGCTTCTGAA CTCTACATAG AGACTGAGAA AAAGAAGCGC 
GAGAAAGCGT ACCTGGTCCG TTCCCAAAGG GCAACAGGCA TTGGAAGGTT GATGGTGAAC 
GTGGTTGAAG GCATCGAGTT GAAACCCTGT CGGTCACATG GAAAGAGCAA CCCGTACTGT 
GAGGTGACCA TGGGTTCCCA GTGCCACATC ACCAAGACGA TCCAGGACAC TCTGAACCCC 
AAGTGGAATT CCAACTGCCA GTTCTTCATC CGAGACCTGG AGCAGGAAGT CCTCTGCATC 
ACTGTGTTCG AGAGGGACCA GTTCTCACCA GATGATTTTT TGGGTCGGAC GGAGATCCGT 
GTGGCGGACA TCAAGAAAGA CCAGGGCTCC AAAGGTCCAG TTACGAAGTG TCTTCTGCTG 
CACGAAGTCC CCACGGGAGA GATTGTGGTC CGCTTGGACC TGCAGTTGTT TGATGAGCCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028432.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883921.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X5, mRNA.

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CloneID	OHu93296
Clone ID Related Accession (Same CDS sequence)	XM_017028432.1 , XM_017028432.2
Accession Version	XM_017028432.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4983bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028432.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GAGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGGCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CCCAGGTTAT TGCCTCATAC 
ACCGCCACCG GCCCCGAGCA GCTCACTCTC GCCCCTGGTC AGCTGATTTT GATCCGAAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTGCAAGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTCC CAGCTAATTA TGTAAAGCTT CTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGAGCCAC CTAAGTCAAC AGCATTAGCG GCAGTGTGCC AGGTGATTGG GATGTACGAC 
TACACCGCGC AGAATGACGA TGAGCTGGCC TTCAACAAGG GCCAGATCAT CAACGTCCTC 
AACAAGGAGG ACCCTGACTG GTGGAAAGGA GAAGTCAATG GACAAGTGGG GCTCTTCCCA 
TCCAATTATG TGAAGCTGAC CACAGACATG GACCCAAGCC AGCAATGGTG TTCAGACTTA 
CATCTCTTGG ATATGTTGAC CCCAACTGAA AGAAAGCGAC AAGGATACAT CCACGAGCTC 
ATTGTCACCG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT TTTTCAAAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGTTG CTATGATTTT TGTGAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTAAAAGCGC TGAGAGTCCG CAAGAAGATG 
TCCGGGGAGA AGATGCCTGT GAAGATGATT GGAGACATCC TGAGCGCACA GCTGCCGCAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCCGC CAGCTCAACG GGGCTGCCCT GATCCAGCAG 
AAGACGGATG AGGCCCCAGA CTTCAAGGAG TTCGTCAAAA GATTGGCAAT GGATCCTCGG 
TGTAAAGGGA TGCCACTCTC TAGTTTTATA CTGAAGCCTA TGCAACGGGT AACAAGATAC 
CCACTGATCA TTAAAAATCA ACTTGTGTTC AATTCAGTGA CCAATTGCTT GGGGCCGCGC 
AAATTTCTGC ACAGTGGGAA GCTCTACAAG GCCAAGAGCA ACAAGGAGCT GTATGGCTTC 
CTTTTCAACG ACTTCCTCCT GCTGACTCAG ATCACGAAGC CTTTGGGGTC TTCTGGCACC 
GACAAAGTCT TCAGCCCCAA ATCAAACCTG CAGTATAAAA TGTATAAAAC ACCTATTTTC 
CTAAATGAGG TTCTAGTAAA ATTACCCACC GACCCTTCTG GAGACGAGCC CATCTTCCAC 
ATCTCCCACA TTGACCGCGT CTATACTCTC CGAGCAGAAA GCATAAATGA AAGGACTGCC 
TGGGTGCAGA AAATCAAAGC TGCTTCTGAA CTCTACATAG AGACTGAGAA AAAGAAGCGC 
GAGAAAGCGT ACCTGGTCCG TTCCCAAAGG GCAACAGGCA TTGGAAGGTT GATGGTGAAC 
GTGGTTGAAG GCATCGAGTT GAAACCCTGT CGGTCACATG GAAAGAGCAA CCCGTACTGT 
GAGGTGACCA TGGGTTCCCA GTGCCACATC ACCAAGACGA TCCAGGACAC TCTGAACCCC 
AAGTGGAATT CCAACTGCCA GTTCTTCATC CGAGACCTGG AGCAGGAAGT CCTCTGCATC 
ACTGTGTTCG AGAGGGACCA GTTCTCACCA GATGATTTTT TGGGTCGGAC GGAGATCCGT 
GTGGCGGACA TCAAGAAAGA CCAGGGCTCC AAAGGTCCAG TTACGAAGTG TCTTCTGCTG 
CACGAAGTCC CCACGGGAGA GATTGTGGTC CGCTTGGACC TGCAGTTGTT TGATGAGCCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883921.1
CDS	294..5276
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028432.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028432.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GAGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGGCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CCCAGGTTAT TGCCTCATAC 
ACCGCCACCG GCCCCGAGCA GCTCACTCTC GCCCCTGGTC AGCTGATTTT GATCCGAAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTGCAAGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTCC CAGCTAATTA TGTAAAGCTT CTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGAGCCAC CTAAGTCAAC AGCATTAGCG GCAGTGTGCC AGGTGATTGG GATGTACGAC 
TACACCGCGC AGAATGACGA TGAGCTGGCC TTCAACAAGG GCCAGATCAT CAACGTCCTC 
AACAAGGAGG ACCCTGACTG GTGGAAAGGA GAAGTCAATG GACAAGTGGG GCTCTTCCCA 
TCCAATTATG TGAAGCTGAC CACAGACATG GACCCAAGCC AGCAATGGTG TTCAGACTTA 
CATCTCTTGG ATATGTTGAC CCCAACTGAA AGAAAGCGAC AAGGATACAT CCACGAGCTC 
ATTGTCACCG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT TTTTCAAAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGTTG CTATGATTTT TGTGAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTAAAAGCGC TGAGAGTCCG CAAGAAGATG 
TCCGGGGAGA AGATGCCTGT GAAGATGATT GGAGACATCC TGAGCGCACA GCTGCCGCAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCCGC CAGCTCAACG GGGCTGCCCT GATCCAGCAG 
AAGACGGATG AGGCCCCAGA CTTCAAGGAG TTCGTCAAAA GATTGGCAAT GGATCCTCGG 
TGTAAAGGGA TGCCACTCTC TAGTTTTATA CTGAAGCCTA TGCAACGGGT AACAAGATAC 
CCACTGATCA TTAAAAATCA ACTTGTGTTC AATTCAGTGA CCAATTGCTT GGGGCCGCGC 
AAATTTCTGC ACAGTGGGAA GCTCTACAAG GCCAAGAGCA ACAAGGAGCT GTATGGCTTC 
CTTTTCAACG ACTTCCTCCT GCTGACTCAG ATCACGAAGC CTTTGGGGTC TTCTGGCACC 
GACAAAGTCT TCAGCCCCAA ATCAAACCTG CAGTATAAAA TGTATAAAAC ACCTATTTTC 
CTAAATGAGG TTCTAGTAAA ATTACCCACC GACCCTTCTG GAGACGAGCC CATCTTCCAC 
ATCTCCCACA TTGACCGCGT CTATACTCTC CGAGCAGAAA GCATAAATGA AAGGACTGCC 
TGGGTGCAGA AAATCAAAGC TGCTTCTGAA CTCTACATAG AGACTGAGAA AAAGAAGCGC 
GAGAAAGCGT ACCTGGTCCG TTCCCAAAGG GCAACAGGCA TTGGAAGGTT GATGGTGAAC 
GTGGTTGAAG GCATCGAGTT GAAACCCTGT CGGTCACATG GAAAGAGCAA CCCGTACTGT 
GAGGTGACCA TGGGTTCCCA GTGCCACATC ACCAAGACGA TCCAGGACAC TCTGAACCCC 
AAGTGGAATT CCAACTGCCA GTTCTTCATC CGAGACCTGG AGCAGGAAGT CCTCTGCATC 
ACTGTGTTCG AGAGGGACCA GTTCTCACCA GATGATTTTT TGGGTCGGAC GGAGATCCGT 
GTGGCGGACA TCAAGAAAGA CCAGGGCTCC AAAGGTCCAG TTACGAAGTG TCTTCTGCTG 
CACGAAGTCC CCACGGGAGA GATTGTGGTC CGCTTGGACC TGCAGTTGTT TGATGAGCCG 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028433.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883922.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X7, mRNA.

Price & Availability↑

CloneID	OHu93297
Clone ID Related Accession (Same CDS sequence)	XM_017028433.1 , XM_017028433.2
Accession Version	XM_017028433.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAAAT TGCCCAGGTT ATTGCCTCAT ACACCGCCAC CGGCCCCGAG 
CAGCTCACTC TCGCCCCTGG TCAGCTGATT TTGATCCGAA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTGCAAGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT CCCAGCTAAT 
TATGTAAAGC TTCTAAGCCC TGGGACGAGC AAAATCACTC CAACAGAGCC ACCTAAGTCA 
ACAGCATTAG CGGCAGTGTG CCAGGTGATT GGGATGTACG ACTACACCGC GCAGAATGAC 
GATGAGCTGG CCTTCAACAA GGGCCAGATC ATCAACGTCC TCAACAAGGA GGACCCTGAC 
TGGTGGAAAG GAGAAGTCAA TGGACAAGTG GGGCTCTTCC CATCCAATTA TGTGAAGCTG 
ACCACAGACA TGGACCCAAG CCAGCAATGG TGTTCAGACT TACATCTCTT GGATATGTTG 
ACCCCAACTG AAAGAAAGCG ACAAGGATAC ATCCACGAGC TCATTGTCAC CGAGGAGAAC 
TATGTGAATG ACCTGCAGCT GGTCACAGAG ATTTTTCAAA AACCCCTGAT GGAGTCTGAG 
CTGCTGACAG AAAAAGAGGT TGCTATGATT TTTGTGAACT GGAAGGAGCT GATTATGTGT 
AATATCAAAC TACTAAAAGC GCTGAGAGTC CGCAAGAAGA TGTCCGGGGA GAAGATGCCT 
GTGAAGATGA TTGGAGACAT CCTGAGCGCA CAGCTGCCGC ACATGCAGCC CTACATCCGC 
TTCTGCAGCC GCCAGCTCAA CGGGGCTGCC CTGATCCAGC AGAAGACGGA TGAGGCCCCA 
GACTTCAAGG AGTTCGTCAA AAGATTGGCA ATGGATCCTC GGTGTAAAGG GATGCCACTC 
TCTAGTTTTA TACTGAAGCC TATGCAACGG GTAACAAGAT ACCCACTGAT CATTAAAAAT 
ATCCTGGAAA ACACCCCTGA AAACCACCCG GACCACAGCC ACTTGAAGCA CGCCCTGGAG 
AAGGCGGAAG AGCTCTGTTC CCAGGTGAAC GAAGGGGTGC GGGAGAAGGA GAACTCTGAC 
CGGCTGGAGT GGATCCAGGC CCACGTGCAG TGTGAAGGCC TGTCTGAGCA ACTTGTGTTC 
AATTCAGTGA CCAATTGCTT GGGGCCGCGC AAATTTCTGC ACAGTGGGAA GCTCTACAAG 
GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTTTTCAACG ACTTCCTCCT GCTGACTCAG 
ATCACGAAGC CTTTGGGGTC TTCTGGCACC GACAAAGTCT TCAGCCCCAA ATCAAACCTG 
CAGTATAAAA TGTATAAAAC ACCTATTTTC CTAAATGAGG TTCTAGTAAA ATTACCCACC 
GACCCTTCTG GAGACGAGCC CATCTTCCAC ATCTCCCACA TTGACCGCGT CTATACTCTC 
CGAGCAGAAA GCATAAATGA AAGGACTGCC TGGGTGCAGA AAATCAAAGC TGCTTCTGAA 
CTCTACATAG AGACTGAGAA AAAGAAGCGC GAGAAAGCGT ACCTGGTCCG TTCCCAAAGG 
GCAACAGGCA TTGGAAGGTT GATGGTGAAC GTGGTTGAAG GCATCGAGTT GAAACCCTGT 
CGGTCACATG GAAAGAGCAA CCCGTACTGT GAGGTGACCA TGGGTTCCCA GTGCCACATC 
ACCAAGACGA TCCAGGACAC TCTGAACCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC 
CGAGACCTGG AGCAGGAAGT CCTCTGCATC ACTGTGTTCG AGAGGGACCA GTTCTCACCA 
GATGATTTTT TGGGTCGGAC GGAGATCCGT GTGGCGGACA TCAAGAAAGA CCAGGGCTCC 
AAAGGTCCAG TTACGAAGTG TCTTCTGCTG CACGAAGTCC CCACGGGAGA GATTGTGGTC 
CGCTTGGACC TGCAGTTGTT TGATGAGCCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883922.1
CDS	347..5299
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028433.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028433.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAAAT TGCCCAGGTT ATTGCCTCAT ACACCGCCAC CGGCCCCGAG 
CAGCTCACTC TCGCCCCTGG TCAGCTGATT TTGATCCGAA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTGCAAGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT CCCAGCTAAT 
TATGTAAAGC TTCTAAGCCC TGGGACGAGC AAAATCACTC CAACAGAGCC ACCTAAGTCA 
ACAGCATTAG CGGCAGTGTG CCAGGTGATT GGGATGTACG ACTACACCGC GCAGAATGAC 
GATGAGCTGG CCTTCAACAA GGGCCAGATC ATCAACGTCC TCAACAAGGA GGACCCTGAC 
TGGTGGAAAG GAGAAGTCAA TGGACAAGTG GGGCTCTTCC CATCCAATTA TGTGAAGCTG 
ACCACAGACA TGGACCCAAG CCAGCAATGG TGTTCAGACT TACATCTCTT GGATATGTTG 
ACCCCAACTG AAAGAAAGCG ACAAGGATAC ATCCACGAGC TCATTGTCAC CGAGGAGAAC 
TATGTGAATG ACCTGCAGCT GGTCACAGAG ATTTTTCAAA AACCCCTGAT GGAGTCTGAG 
CTGCTGACAG AAAAAGAGGT TGCTATGATT TTTGTGAACT GGAAGGAGCT GATTATGTGT 
AATATCAAAC TACTAAAAGC GCTGAGAGTC CGCAAGAAGA TGTCCGGGGA GAAGATGCCT 
GTGAAGATGA TTGGAGACAT CCTGAGCGCA CAGCTGCCGC ACATGCAGCC CTACATCCGC 
TTCTGCAGCC GCCAGCTCAA CGGGGCTGCC CTGATCCAGC AGAAGACGGA TGAGGCCCCA 
GACTTCAAGG AGTTCGTCAA AAGATTGGCA ATGGATCCTC GGTGTAAAGG GATGCCACTC 
TCTAGTTTTA TACTGAAGCC TATGCAACGG GTAACAAGAT ACCCACTGAT CATTAAAAAT 
ATCCTGGAAA ACACCCCTGA AAACCACCCG GACCACAGCC ACTTGAAGCA CGCCCTGGAG 
AAGGCGGAAG AGCTCTGTTC CCAGGTGAAC GAAGGGGTGC GGGAGAAGGA GAACTCTGAC 
CGGCTGGAGT GGATCCAGGC CCACGTGCAG TGTGAAGGCC TGTCTGAGCA ACTTGTGTTC 
AATTCAGTGA CCAATTGCTT GGGGCCGCGC AAATTTCTGC ACAGTGGGAA GCTCTACAAG 
GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTTTTCAACG ACTTCCTCCT GCTGACTCAG 
ATCACGAAGC CTTTGGGGTC TTCTGGCACC GACAAAGTCT TCAGCCCCAA ATCAAACCTG 
CAGTATAAAA TGTATAAAAC ACCTATTTTC CTAAATGAGG TTCTAGTAAA ATTACCCACC 
GACCCTTCTG GAGACGAGCC CATCTTCCAC ATCTCCCACA TTGACCGCGT CTATACTCTC 
CGAGCAGAAA GCATAAATGA AAGGACTGCC TGGGTGCAGA AAATCAAAGC TGCTTCTGAA 
CTCTACATAG AGACTGAGAA AAAGAAGCGC GAGAAAGCGT ACCTGGTCCG TTCCCAAAGG 
GCAACAGGCA TTGGAAGGTT GATGGTGAAC GTGGTTGAAG GCATCGAGTT GAAACCCTGT 
CGGTCACATG GAAAGAGCAA CCCGTACTGT GAGGTGACCA TGGGTTCCCA GTGCCACATC 
ACCAAGACGA TCCAGGACAC TCTGAACCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC 
CGAGACCTGG AGCAGGAAGT CCTCTGCATC ACTGTGTTCG AGAGGGACCA GTTCTCACCA 
GATGATTTTT TGGGTCGGAC GGAGATCCGT GTGGCGGACA TCAAGAAAGA CCAGGGCTCC 
AAAGGTCCAG TTACGAAGTG TCTTCTGCTG CACGAAGTCC CCACGGGAGA GATTGTGGTC 
CGCTTGGACC TGCAGTTGTT TGATGAGCCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028433.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883922.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X6, mRNA.

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CloneID	OHu93297
Clone ID Related Accession (Same CDS sequence)	XM_017028433.1 , XM_017028433.2
Accession Version	XM_017028433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4953bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028433.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAAAT TGCCCAGGTT ATTGCCTCAT ACACCGCCAC CGGCCCCGAG 
CAGCTCACTC TCGCCCCTGG TCAGCTGATT TTGATCCGAA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTGCAAGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT CCCAGCTAAT 
TATGTAAAGC TTCTAAGCCC TGGGACGAGC AAAATCACTC CAACAGAGCC ACCTAAGTCA 
ACAGCATTAG CGGCAGTGTG CCAGGTGATT GGGATGTACG ACTACACCGC GCAGAATGAC 
GATGAGCTGG CCTTCAACAA GGGCCAGATC ATCAACGTCC TCAACAAGGA GGACCCTGAC 
TGGTGGAAAG GAGAAGTCAA TGGACAAGTG GGGCTCTTCC CATCCAATTA TGTGAAGCTG 
ACCACAGACA TGGACCCAAG CCAGCAATGG TGTTCAGACT TACATCTCTT GGATATGTTG 
ACCCCAACTG AAAGAAAGCG ACAAGGATAC ATCCACGAGC TCATTGTCAC CGAGGAGAAC 
TATGTGAATG ACCTGCAGCT GGTCACAGAG ATTTTTCAAA AACCCCTGAT GGAGTCTGAG 
CTGCTGACAG AAAAAGAGGT TGCTATGATT TTTGTGAACT GGAAGGAGCT GATTATGTGT 
AATATCAAAC TACTAAAAGC GCTGAGAGTC CGCAAGAAGA TGTCCGGGGA GAAGATGCCT 
GTGAAGATGA TTGGAGACAT CCTGAGCGCA CAGCTGCCGC ACATGCAGCC CTACATCCGC 
TTCTGCAGCC GCCAGCTCAA CGGGGCTGCC CTGATCCAGC AGAAGACGGA TGAGGCCCCA 
GACTTCAAGG AGTTCGTCAA AAGATTGGCA ATGGATCCTC GGTGTAAAGG GATGCCACTC 
TCTAGTTTTA TACTGAAGCC TATGCAACGG GTAACAAGAT ACCCACTGAT CATTAAAAAT 
ATCCTGGAAA ACACCCCTGA AAACCACCCG GACCACAGCC ACTTGAAGCA CGCCCTGGAG 
AAGGCGGAAG AGCTCTGTTC CCAGGTGAAC GAAGGGGTGC GGGAGAAGGA GAACTCTGAC 
CGGCTGGAGT GGATCCAGGC CCACGTGCAG TGTGAAGGCC TGTCTGAGCA ACTTGTGTTC 
AATTCAGTGA CCAATTGCTT GGGGCCGCGC AAATTTCTGC ACAGTGGGAA GCTCTACAAG 
GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTTTTCAACG ACTTCCTCCT GCTGACTCAG 
ATCACGAAGC CTTTGGGGTC TTCTGGCACC GACAAAGTCT TCAGCCCCAA ATCAAACCTG 
CAGTATAAAA TGTATAAAAC ACCTATTTTC CTAAATGAGG TTCTAGTAAA ATTACCCACC 
GACCCTTCTG GAGACGAGCC CATCTTCCAC ATCTCCCACA TTGACCGCGT CTATACTCTC 
CGAGCAGAAA GCATAAATGA AAGGACTGCC TGGGTGCAGA AAATCAAAGC TGCTTCTGAA 
CTCTACATAG AGACTGAGAA AAAGAAGCGC GAGAAAGCGT ACCTGGTCCG TTCCCAAAGG 
GCAACAGGCA TTGGAAGGTT GATGGTGAAC GTGGTTGAAG GCATCGAGTT GAAACCCTGT 
CGGTCACATG GAAAGAGCAA CCCGTACTGT GAGGTGACCA TGGGTTCCCA GTGCCACATC 
ACCAAGACGA TCCAGGACAC TCTGAACCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC 
CGAGACCTGG AGCAGGAAGT CCTCTGCATC ACTGTGTTCG AGAGGGACCA GTTCTCACCA 
GATGATTTTT TGGGTCGGAC GGAGATCCGT GTGGCGGACA TCAAGAAAGA CCAGGGCTCC 
AAAGGTCCAG TTACGAAGTG TCTTCTGCTG CACGAAGTCC CCACGGGAGA GATTGTGGTC 
CGCTTGGACC TGCAGTTGTT TGATGAGCCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883922.1
CDS	294..5246
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028433.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028433.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAAAT TGCCCAGGTT ATTGCCTCAT ACACCGCCAC CGGCCCCGAG 
CAGCTCACTC TCGCCCCTGG TCAGCTGATT TTGATCCGAA AAAAGAACCC AGGTGGATGG 
TGGGAAGGAG AGCTGCAAGC ACGTGGGAAA AAGCGCCAGA TAGGCTGGTT CCCAGCTAAT 
TATGTAAAGC TTCTAAGCCC TGGGACGAGC AAAATCACTC CAACAGAGCC ACCTAAGTCA 
ACAGCATTAG CGGCAGTGTG CCAGGTGATT GGGATGTACG ACTACACCGC GCAGAATGAC 
GATGAGCTGG CCTTCAACAA GGGCCAGATC ATCAACGTCC TCAACAAGGA GGACCCTGAC 
TGGTGGAAAG GAGAAGTCAA TGGACAAGTG GGGCTCTTCC CATCCAATTA TGTGAAGCTG 
ACCACAGACA TGGACCCAAG CCAGCAATGG TGTTCAGACT TACATCTCTT GGATATGTTG 
ACCCCAACTG AAAGAAAGCG ACAAGGATAC ATCCACGAGC TCATTGTCAC CGAGGAGAAC 
TATGTGAATG ACCTGCAGCT GGTCACAGAG ATTTTTCAAA AACCCCTGAT GGAGTCTGAG 
CTGCTGACAG AAAAAGAGGT TGCTATGATT TTTGTGAACT GGAAGGAGCT GATTATGTGT 
AATATCAAAC TACTAAAAGC GCTGAGAGTC CGCAAGAAGA TGTCCGGGGA GAAGATGCCT 
GTGAAGATGA TTGGAGACAT CCTGAGCGCA CAGCTGCCGC ACATGCAGCC CTACATCCGC 
TTCTGCAGCC GCCAGCTCAA CGGGGCTGCC CTGATCCAGC AGAAGACGGA TGAGGCCCCA 
GACTTCAAGG AGTTCGTCAA AAGATTGGCA ATGGATCCTC GGTGTAAAGG GATGCCACTC 
TCTAGTTTTA TACTGAAGCC TATGCAACGG GTAACAAGAT ACCCACTGAT CATTAAAAAT 
ATCCTGGAAA ACACCCCTGA AAACCACCCG GACCACAGCC ACTTGAAGCA CGCCCTGGAG 
AAGGCGGAAG AGCTCTGTTC CCAGGTGAAC GAAGGGGTGC GGGAGAAGGA GAACTCTGAC 
CGGCTGGAGT GGATCCAGGC CCACGTGCAG TGTGAAGGCC TGTCTGAGCA ACTTGTGTTC 
AATTCAGTGA CCAATTGCTT GGGGCCGCGC AAATTTCTGC ACAGTGGGAA GCTCTACAAG 
GCCAAGAGCA ACAAGGAGCT GTATGGCTTC CTTTTCAACG ACTTCCTCCT GCTGACTCAG 
ATCACGAAGC CTTTGGGGTC TTCTGGCACC GACAAAGTCT TCAGCCCCAA ATCAAACCTG 
CAGTATAAAA TGTATAAAAC ACCTATTTTC CTAAATGAGG TTCTAGTAAA ATTACCCACC 
GACCCTTCTG GAGACGAGCC CATCTTCCAC ATCTCCCACA TTGACCGCGT CTATACTCTC 
CGAGCAGAAA GCATAAATGA AAGGACTGCC TGGGTGCAGA AAATCAAAGC TGCTTCTGAA 
CTCTACATAG AGACTGAGAA AAAGAAGCGC GAGAAAGCGT ACCTGGTCCG TTCCCAAAGG 
GCAACAGGCA TTGGAAGGTT GATGGTGAAC GTGGTTGAAG GCATCGAGTT GAAACCCTGT 
CGGTCACATG GAAAGAGCAA CCCGTACTGT GAGGTGACCA TGGGTTCCCA GTGCCACATC 
ACCAAGACGA TCCAGGACAC TCTGAACCCC AAGTGGAATT CCAACTGCCA GTTCTTCATC 
CGAGACCTGG AGCAGGAAGT CCTCTGCATC ACTGTGTTCG AGAGGGACCA GTTCTCACCA 
GATGATTTTT TGGGTCGGAC GGAGATCCGT GTGGCGGACA TCAAGAAAGA CCAGGGCTCC 
AAAGGTCCAG TTACGAAGTG TCTTCTGCTG CACGAAGTCC CCACGGGAGA GATTGTGGTC 
CGCTTGGACC TGCAGTTGTT TGATGAGCCG TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028434.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883923.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X8, mRNA.

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CloneID	OHu93298
Clone ID Related Accession (Same CDS sequence)	XM_017028434.1 , XM_017028434.2
Accession Version	XM_017028434.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAAATTGCCC AGGTTATTGC CTCATACACC GCCACCGGCC CCGAGCAGCT CACTCTCGCC 
CCTGGTCAGC TGATTTTGAT CCGAAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTG 
CAAGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTCCCAG CTAATTATGT AAAGCTTCTA 
AGCCCTGGGA CGAGCAAAAT CACTCCAACA GAGCCACCTA AGTCAACAGC ATTAGCGGCA 
GTGTGCCAGG TGATTGGGAT GTACGACTAC ACCGCGCAGA ATGACGATGA GCTGGCCTTC 
AACAAGGGCC AGATCATCAA CGTCCTCAAC AAGGAGGACC CTGACTGGTG GAAAGGAGAA 
GTCAATGGAC AAGTGGGGCT CTTCCCATCC AATTATGTGA AGCTGACCAC AGACATGGAC 
CCAAGCCAGC AATGGTGTTC AGACTTACAT CTCTTGGATA TGTTGACCCC AACTGAAAGA 
AAGCGACAAG GATACATCCA CGAGCTCATT GTCACCGAGG AGAACTATGT GAATGACCTG 
CAGCTGGTCA CAGAGATTTT TCAAAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA 
GAGGTTGCTA TGATTTTTGT GAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTA 
AAAGCGCTGA GAGTCCGCAA GAAGATGTCC GGGGAGAAGA TGCCTGTGAA GATGATTGGA 
GACATCCTGA GCGCACAGCT GCCGCACATG CAGCCCTACA TCCGCTTCTG CAGCCGCCAG 
CTCAACGGGG CTGCCCTGAT CCAGCAGAAG ACGGATGAGG CCCCAGACTT CAAGGAGTTC 
GTCAAAAGAT TGGCAATGGA TCCTCGGTGT AAAGGGATGC CACTCTCTAG TTTTATACTG 
AAGCCTATGC AACGGGTAAC AAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACC 
CCTGAAAACC ACCCGGACCA CAGCCACTTG AAGCACGCCC TGGAGAAGGC GGAAGAGCTC 
TGTTCCCAGG TGAACGAAGG GGTGCGGGAG AAGGAGAACT CTGACCGGCT GGAGTGGATC 
CAGGCCCACG TGCAGTGTGA AGGCCTGTCT GAGCAACTTG TGTTCAATTC AGTGACCAAT 
TGCTTGGGGC CGCGCAAATT TCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTTTT CAACGACTTC CTCCTGCTGA CTCAGATCAC GAAGCCTTTG 
GGGTCTTCTG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAT 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTAAAATTAC CCACCGACCC TTCTGGAGAC 
GAGCCCATCT TCCACATCTC CCACATTGAC CGCGTCTATA CTCTCCGAGC AGAAAGCATA 
AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCTGCTT CTGAACTCTA CATAGAGACT 
GAGAAAAAGA AGCGCGAGAA AGCGTACCTG GTCCGTTCCC AAAGGGCAAC AGGCATTGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATC GAGTTGAAAC CCTGTCGGTC ACATGGAAAG 
AGCAACCCGT ACTGTGAGGT GACCATGGGT TCCCAGTGCC ACATCACCAA GACGATCCAG 
GACACTCTGA ACCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCCGAGA CCTGGAGCAG 
GAAGTCCTCT GCATCACTGT GTTCGAGAGG GACCAGTTCT CACCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGTGTGGC GGACATCAAG AAAGACCAGG GCTCCAAAGG TCCAGTTACG 
AAGTGTCTTC TGCTGCACGA AGTCCCCACG GGAGAGATTG TGGTCCGCTT GGACCTGCAG 
TTGTTTGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883923.1
CDS	347..5284
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028434.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028434.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAAATTGCCC AGGTTATTGC CTCATACACC GCCACCGGCC CCGAGCAGCT CACTCTCGCC 
CCTGGTCAGC TGATTTTGAT CCGAAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTG 
CAAGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTCCCAG CTAATTATGT AAAGCTTCTA 
AGCCCTGGGA CGAGCAAAAT CACTCCAACA GAGCCACCTA AGTCAACAGC ATTAGCGGCA 
GTGTGCCAGG TGATTGGGAT GTACGACTAC ACCGCGCAGA ATGACGATGA GCTGGCCTTC 
AACAAGGGCC AGATCATCAA CGTCCTCAAC AAGGAGGACC CTGACTGGTG GAAAGGAGAA 
GTCAATGGAC AAGTGGGGCT CTTCCCATCC AATTATGTGA AGCTGACCAC AGACATGGAC 
CCAAGCCAGC AATGGTGTTC AGACTTACAT CTCTTGGATA TGTTGACCCC AACTGAAAGA 
AAGCGACAAG GATACATCCA CGAGCTCATT GTCACCGAGG AGAACTATGT GAATGACCTG 
CAGCTGGTCA CAGAGATTTT TCAAAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA 
GAGGTTGCTA TGATTTTTGT GAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTA 
AAAGCGCTGA GAGTCCGCAA GAAGATGTCC GGGGAGAAGA TGCCTGTGAA GATGATTGGA 
GACATCCTGA GCGCACAGCT GCCGCACATG CAGCCCTACA TCCGCTTCTG CAGCCGCCAG 
CTCAACGGGG CTGCCCTGAT CCAGCAGAAG ACGGATGAGG CCCCAGACTT CAAGGAGTTC 
GTCAAAAGAT TGGCAATGGA TCCTCGGTGT AAAGGGATGC CACTCTCTAG TTTTATACTG 
AAGCCTATGC AACGGGTAAC AAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACC 
CCTGAAAACC ACCCGGACCA CAGCCACTTG AAGCACGCCC TGGAGAAGGC GGAAGAGCTC 
TGTTCCCAGG TGAACGAAGG GGTGCGGGAG AAGGAGAACT CTGACCGGCT GGAGTGGATC 
CAGGCCCACG TGCAGTGTGA AGGCCTGTCT GAGCAACTTG TGTTCAATTC AGTGACCAAT 
TGCTTGGGGC CGCGCAAATT TCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTTTT CAACGACTTC CTCCTGCTGA CTCAGATCAC GAAGCCTTTG 
GGGTCTTCTG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAT 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTAAAATTAC CCACCGACCC TTCTGGAGAC 
GAGCCCATCT TCCACATCTC CCACATTGAC CGCGTCTATA CTCTCCGAGC AGAAAGCATA 
AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCTGCTT CTGAACTCTA CATAGAGACT 
GAGAAAAAGA AGCGCGAGAA AGCGTACCTG GTCCGTTCCC AAAGGGCAAC AGGCATTGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATC GAGTTGAAAC CCTGTCGGTC ACATGGAAAG 
AGCAACCCGT ACTGTGAGGT GACCATGGGT TCCCAGTGCC ACATCACCAA GACGATCCAG 
GACACTCTGA ACCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCCGAGA CCTGGAGCAG 
GAAGTCCTCT GCATCACTGT GTTCGAGAGG GACCAGTTCT CACCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGTGTGGC GGACATCAAG AAAGACCAGG GCTCCAAAGG TCCAGTTACG 
AAGTGTCTTC TGCTGCACGA AGTCCCCACG GGAGAGATTG TGGTCCGCTT GGACCTGCAG 
TTGTTTGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028434.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883923.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X7, mRNA.

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CloneID	OHu93298
Clone ID Related Accession (Same CDS sequence)	XM_017028434.1 , XM_017028434.2
Accession Version	XM_017028434.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4938bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028434.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAAATTGCCC AGGTTATTGC CTCATACACC GCCACCGGCC CCGAGCAGCT CACTCTCGCC 
CCTGGTCAGC TGATTTTGAT CCGAAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTG 
CAAGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTCCCAG CTAATTATGT AAAGCTTCTA 
AGCCCTGGGA CGAGCAAAAT CACTCCAACA GAGCCACCTA AGTCAACAGC ATTAGCGGCA 
GTGTGCCAGG TGATTGGGAT GTACGACTAC ACCGCGCAGA ATGACGATGA GCTGGCCTTC 
AACAAGGGCC AGATCATCAA CGTCCTCAAC AAGGAGGACC CTGACTGGTG GAAAGGAGAA 
GTCAATGGAC AAGTGGGGCT CTTCCCATCC AATTATGTGA AGCTGACCAC AGACATGGAC 
CCAAGCCAGC AATGGTGTTC AGACTTACAT CTCTTGGATA TGTTGACCCC AACTGAAAGA 
AAGCGACAAG GATACATCCA CGAGCTCATT GTCACCGAGG AGAACTATGT GAATGACCTG 
CAGCTGGTCA CAGAGATTTT TCAAAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA 
GAGGTTGCTA TGATTTTTGT GAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTA 
AAAGCGCTGA GAGTCCGCAA GAAGATGTCC GGGGAGAAGA TGCCTGTGAA GATGATTGGA 
GACATCCTGA GCGCACAGCT GCCGCACATG CAGCCCTACA TCCGCTTCTG CAGCCGCCAG 
CTCAACGGGG CTGCCCTGAT CCAGCAGAAG ACGGATGAGG CCCCAGACTT CAAGGAGTTC 
GTCAAAAGAT TGGCAATGGA TCCTCGGTGT AAAGGGATGC CACTCTCTAG TTTTATACTG 
AAGCCTATGC AACGGGTAAC AAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACC 
CCTGAAAACC ACCCGGACCA CAGCCACTTG AAGCACGCCC TGGAGAAGGC GGAAGAGCTC 
TGTTCCCAGG TGAACGAAGG GGTGCGGGAG AAGGAGAACT CTGACCGGCT GGAGTGGATC 
CAGGCCCACG TGCAGTGTGA AGGCCTGTCT GAGCAACTTG TGTTCAATTC AGTGACCAAT 
TGCTTGGGGC CGCGCAAATT TCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTTTT CAACGACTTC CTCCTGCTGA CTCAGATCAC GAAGCCTTTG 
GGGTCTTCTG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAT 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTAAAATTAC CCACCGACCC TTCTGGAGAC 
GAGCCCATCT TCCACATCTC CCACATTGAC CGCGTCTATA CTCTCCGAGC AGAAAGCATA 
AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCTGCTT CTGAACTCTA CATAGAGACT 
GAGAAAAAGA AGCGCGAGAA AGCGTACCTG GTCCGTTCCC AAAGGGCAAC AGGCATTGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATC GAGTTGAAAC CCTGTCGGTC ACATGGAAAG 
AGCAACCCGT ACTGTGAGGT GACCATGGGT TCCCAGTGCC ACATCACCAA GACGATCCAG 
GACACTCTGA ACCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCCGAGA CCTGGAGCAG 
GAAGTCCTCT GCATCACTGT GTTCGAGAGG GACCAGTTCT CACCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGTGTGGC GGACATCAAG AAAGACCAGG GCTCCAAAGG TCCAGTTACG 
AAGTGTCTTC TGCTGCACGA AGTCCCCACG GGAGAGATTG TGGTCCGCTT GGACCTGCAG 
TTGTTTGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883923.1
CDS	294..5231
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028434.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028434.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAAATTGCCC AGGTTATTGC CTCATACACC GCCACCGGCC CCGAGCAGCT CACTCTCGCC 
CCTGGTCAGC TGATTTTGAT CCGAAAAAAG AACCCAGGTG GATGGTGGGA AGGAGAGCTG 
CAAGCACGTG GGAAAAAGCG CCAGATAGGC TGGTTCCCAG CTAATTATGT AAAGCTTCTA 
AGCCCTGGGA CGAGCAAAAT CACTCCAACA GAGCCACCTA AGTCAACAGC ATTAGCGGCA 
GTGTGCCAGG TGATTGGGAT GTACGACTAC ACCGCGCAGA ATGACGATGA GCTGGCCTTC 
AACAAGGGCC AGATCATCAA CGTCCTCAAC AAGGAGGACC CTGACTGGTG GAAAGGAGAA 
GTCAATGGAC AAGTGGGGCT CTTCCCATCC AATTATGTGA AGCTGACCAC AGACATGGAC 
CCAAGCCAGC AATGGTGTTC AGACTTACAT CTCTTGGATA TGTTGACCCC AACTGAAAGA 
AAGCGACAAG GATACATCCA CGAGCTCATT GTCACCGAGG AGAACTATGT GAATGACCTG 
CAGCTGGTCA CAGAGATTTT TCAAAAACCC CTGATGGAGT CTGAGCTGCT GACAGAAAAA 
GAGGTTGCTA TGATTTTTGT GAACTGGAAG GAGCTGATTA TGTGTAATAT CAAACTACTA 
AAAGCGCTGA GAGTCCGCAA GAAGATGTCC GGGGAGAAGA TGCCTGTGAA GATGATTGGA 
GACATCCTGA GCGCACAGCT GCCGCACATG CAGCCCTACA TCCGCTTCTG CAGCCGCCAG 
CTCAACGGGG CTGCCCTGAT CCAGCAGAAG ACGGATGAGG CCCCAGACTT CAAGGAGTTC 
GTCAAAAGAT TGGCAATGGA TCCTCGGTGT AAAGGGATGC CACTCTCTAG TTTTATACTG 
AAGCCTATGC AACGGGTAAC AAGATACCCA CTGATCATTA AAAATATCCT GGAAAACACC 
CCTGAAAACC ACCCGGACCA CAGCCACTTG AAGCACGCCC TGGAGAAGGC GGAAGAGCTC 
TGTTCCCAGG TGAACGAAGG GGTGCGGGAG AAGGAGAACT CTGACCGGCT GGAGTGGATC 
CAGGCCCACG TGCAGTGTGA AGGCCTGTCT GAGCAACTTG TGTTCAATTC AGTGACCAAT 
TGCTTGGGGC CGCGCAAATT TCTGCACAGT GGGAAGCTCT ACAAGGCCAA GAGCAACAAG 
GAGCTGTATG GCTTCCTTTT CAACGACTTC CTCCTGCTGA CTCAGATCAC GAAGCCTTTG 
GGGTCTTCTG GCACCGACAA AGTCTTCAGC CCCAAATCAA ACCTGCAGTA TAAAATGTAT 
AAAACACCTA TTTTCCTAAA TGAGGTTCTA GTAAAATTAC CCACCGACCC TTCTGGAGAC 
GAGCCCATCT TCCACATCTC CCACATTGAC CGCGTCTATA CTCTCCGAGC AGAAAGCATA 
AATGAAAGGA CTGCCTGGGT GCAGAAAATC AAAGCTGCTT CTGAACTCTA CATAGAGACT 
GAGAAAAAGA AGCGCGAGAA AGCGTACCTG GTCCGTTCCC AAAGGGCAAC AGGCATTGGA 
AGGTTGATGG TGAACGTGGT TGAAGGCATC GAGTTGAAAC CCTGTCGGTC ACATGGAAAG 
AGCAACCCGT ACTGTGAGGT GACCATGGGT TCCCAGTGCC ACATCACCAA GACGATCCAG 
GACACTCTGA ACCCCAAGTG GAATTCCAAC TGCCAGTTCT TCATCCGAGA CCTGGAGCAG 
GAAGTCCTCT GCATCACTGT GTTCGAGAGG GACCAGTTCT CACCAGATGA TTTTTTGGGT 
CGGACGGAGA TCCGTGTGGC GGACATCAAG AAAGACCAGG GCTCCAAAGG TCCAGTTACG 
AAGTGTCTTC TGCTGCACGA AGTCCCCACG GGAGAGATTG TGGTCCGCTT GGACCTGCAG 
TTGTTTGATG AGCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028435.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883924.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X9, mRNA.

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CloneID	OHu93299
Clone ID Related Accession (Same CDS sequence)	XM_017028435.1 , XM_017028435.2
Accession Version	XM_017028435.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAAATT GCCCAGGTTA TTGCCTCATA CACCGCCACC 
GGCCCCGAGC AGCTCACTCT CGCCCCTGGT CAGCTGATTT TGATCCGAAA AAAGAACCCA 
GGTGGATGGT GGGAAGGAGA GCTGCAAGCA CGTGGGAAAA AGCGCCAGAT AGGCTGGTTC 
CCAGCTAATT ATGTAAAGCT TCTAAGCCCT GGGACGAGCA AAATCACTCC AACAGAGCCA 
CCTAAGTCAA CAGCATTAGC GGCAGTGTGC CAGGTGATTG GGATGTACGA CTACACCGCG 
CAGAATGACG ATGAGCTGGC CTTCAACAAG GGCCAGATCA TCAACGTCCT CAACAAGGAG 
GACCCTGACT GGTGGAAAGG AGAAGTCAAT GGACAAGTGG GGCTCTTCCC ATCCAATTAT 
GTGAAGCTGA CCACAGACAT GGACCCAAGC CAGCAATGGT GTTCAGACTT ACATCTCTTG 
GATATGTTGA CCCCAACTGA AAGAAAGCGA CAAGGATACA TCCACGAGCT CATTGTCACC 
GAGGAGAACT ATGTGAATGA CCTGCAGCTG GTCACAGAGA TTTTTCAAAA ACCCCTGATG 
GAGTCTGAGC TGCTGACAGA AAAAGAGGTT GCTATGATTT TTGTGAACTG GAAGGAGCTG 
ATTATGTGTA ATATCAAACT ACTAAAAGCG CTGAGAGTCC GCAAGAAGAT GTCCGGGGAG 
AAGATGCCTG TGAAGATGAT TGGAGACATC CTGAGCGCAC AGCTGCCGCA CATGCAGCCC 
TACATCCGCT TCTGCAGCCG CCAGCTCAAC GGGGCTGCCC TGATCCAGCA GAAGACGGAT 
GAGGCCCCAG ACTTCAAGGA GTTCGTCAAA AGATTGGCAA TGGATCCTCG GTGTAAAGGG 
ATGCCACTCT CTAGTTTTAT ACTGAAGCCT ATGCAACGGG TAACAAGATA CCCACTGATC 
ATTAAAAATA TCCTGGAAAA CACCCCTGAA AACCACCCGG ACCACAGCCA CTTGAAGCAC 
GCCCTGGAGA AGGCGGAAGA GCTCTGTTCC CAGGTGAACG AAGGGGTGCG GGAGAAGGAG 
AACTCTGACC GGCTGGAGTG GATCCAGGCC CACGTGCAGT GTGAAGGCCT GTCTGAGCAA 
CTTGTGTTCA ATTCAGTGAC CAATTGCTTG GGGCCGCGCA AATTTCTGCA CAGTGGGAAG 
CTCTACAAGG CCAAGAGCAA CAAGGAGCTG TATGGCTTCC TTTTCAACGA CTTCCTCCTG 
CTGACTCAGA TCACGAAGCC TTTGGGGTCT TCTGGCACCG ACAAAGTCTT CAGCCCCAAA 
TCAAACCTGC AGTATAAAAT GTATAAAACA CCTATTTTCC TAAATGAGGT TCTAGTAAAA 
TTACCCACCG ACCCTTCTGG AGACGAGCCC ATCTTCCACA TCTCCCACAT TGACCGCGTC 
TATACTCTCC GAGCAGAAAG CATAAATGAA AGGACTGCCT GGGTGCAGAA AATCAAAGCT 
GCTTCTGAAC TCTACATAGA GACTGAGAAA AAGAAGCGCG AGAAAGCGTA CCTGGTCCGT 
TCCCAAAGGG CAACAGGCAT TGGAAGGTTG ATGGTGAACG TGGTTGAAGG CATCGAGTTG 
AAACCCTGTC GGTCACATGG AAAGAGCAAC CCGTACTGTG AGGTGACCAT GGGTTCCCAG 
TGCCACATCA CCAAGACGAT CCAGGACACT CTGAACCCCA AGTGGAATTC CAACTGCCAG 
TTCTTCATCC GAGACCTGGA GCAGGAAGTC CTCTGCATCA CTGTGTTCGA GAGGGACCAG 
TTCTCACCAG ATGATTTTTT GGGTCGGACG GAGATCCGTG TGGCGGACAT CAAGAAAGAC 
CAGGGCTCCA AAGGTCCAGT TACGAAGTGT CTTCTGCTGC ACGAAGTCCC CACGGGAGAG 
ATTGTGGTCC GCTTGGACCT GCAGTTGTTT GATGAGCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883924.1
CDS	347..5188
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028435.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028435.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAAATT GCCCAGGTTA TTGCCTCATA CACCGCCACC 
GGCCCCGAGC AGCTCACTCT CGCCCCTGGT CAGCTGATTT TGATCCGAAA AAAGAACCCA 
GGTGGATGGT GGGAAGGAGA GCTGCAAGCA CGTGGGAAAA AGCGCCAGAT AGGCTGGTTC 
CCAGCTAATT ATGTAAAGCT TCTAAGCCCT GGGACGAGCA AAATCACTCC AACAGAGCCA 
CCTAAGTCAA CAGCATTAGC GGCAGTGTGC CAGGTGATTG GGATGTACGA CTACACCGCG 
CAGAATGACG ATGAGCTGGC CTTCAACAAG GGCCAGATCA TCAACGTCCT CAACAAGGAG 
GACCCTGACT GGTGGAAAGG AGAAGTCAAT GGACAAGTGG GGCTCTTCCC ATCCAATTAT 
GTGAAGCTGA CCACAGACAT GGACCCAAGC CAGCAATGGT GTTCAGACTT ACATCTCTTG 
GATATGTTGA CCCCAACTGA AAGAAAGCGA CAAGGATACA TCCACGAGCT CATTGTCACC 
GAGGAGAACT ATGTGAATGA CCTGCAGCTG GTCACAGAGA TTTTTCAAAA ACCCCTGATG 
GAGTCTGAGC TGCTGACAGA AAAAGAGGTT GCTATGATTT TTGTGAACTG GAAGGAGCTG 
ATTATGTGTA ATATCAAACT ACTAAAAGCG CTGAGAGTCC GCAAGAAGAT GTCCGGGGAG 
AAGATGCCTG TGAAGATGAT TGGAGACATC CTGAGCGCAC AGCTGCCGCA CATGCAGCCC 
TACATCCGCT TCTGCAGCCG CCAGCTCAAC GGGGCTGCCC TGATCCAGCA GAAGACGGAT 
GAGGCCCCAG ACTTCAAGGA GTTCGTCAAA AGATTGGCAA TGGATCCTCG GTGTAAAGGG 
ATGCCACTCT CTAGTTTTAT ACTGAAGCCT ATGCAACGGG TAACAAGATA CCCACTGATC 
ATTAAAAATA TCCTGGAAAA CACCCCTGAA AACCACCCGG ACCACAGCCA CTTGAAGCAC 
GCCCTGGAGA AGGCGGAAGA GCTCTGTTCC CAGGTGAACG AAGGGGTGCG GGAGAAGGAG 
AACTCTGACC GGCTGGAGTG GATCCAGGCC CACGTGCAGT GTGAAGGCCT GTCTGAGCAA 
CTTGTGTTCA ATTCAGTGAC CAATTGCTTG GGGCCGCGCA AATTTCTGCA CAGTGGGAAG 
CTCTACAAGG CCAAGAGCAA CAAGGAGCTG TATGGCTTCC TTTTCAACGA CTTCCTCCTG 
CTGACTCAGA TCACGAAGCC TTTGGGGTCT TCTGGCACCG ACAAAGTCTT CAGCCCCAAA 
TCAAACCTGC AGTATAAAAT GTATAAAACA CCTATTTTCC TAAATGAGGT TCTAGTAAAA 
TTACCCACCG ACCCTTCTGG AGACGAGCCC ATCTTCCACA TCTCCCACAT TGACCGCGTC 
TATACTCTCC GAGCAGAAAG CATAAATGAA AGGACTGCCT GGGTGCAGAA AATCAAAGCT 
GCTTCTGAAC TCTACATAGA GACTGAGAAA AAGAAGCGCG AGAAAGCGTA CCTGGTCCGT 
TCCCAAAGGG CAACAGGCAT TGGAAGGTTG ATGGTGAACG TGGTTGAAGG CATCGAGTTG 
AAACCCTGTC GGTCACATGG AAAGAGCAAC CCGTACTGTG AGGTGACCAT GGGTTCCCAG 
TGCCACATCA CCAAGACGAT CCAGGACACT CTGAACCCCA AGTGGAATTC CAACTGCCAG 
TTCTTCATCC GAGACCTGGA GCAGGAAGTC CTCTGCATCA CTGTGTTCGA GAGGGACCAG 
TTCTCACCAG ATGATTTTTT GGGTCGGACG GAGATCCGTG TGGCGGACAT CAAGAAAGAC 
CAGGGCTCCA AAGGTCCAGT TACGAAGTGT CTTCTGCTGC ACGAAGTCCC CACGGGAGAG 
ATTGTGGTCC GCTTGGACCT GCAGTTGTTT GATGAGCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028435.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883924.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X8, mRNA.

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CloneID	OHu93299
Clone ID Related Accession (Same CDS sequence)	XM_017028435.1 , XM_017028435.2
Accession Version	XM_017028435.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4842bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028435.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAAATT GCCCAGGTTA TTGCCTCATA CACCGCCACC 
GGCCCCGAGC AGCTCACTCT CGCCCCTGGT CAGCTGATTT TGATCCGAAA AAAGAACCCA 
GGTGGATGGT GGGAAGGAGA GCTGCAAGCA CGTGGGAAAA AGCGCCAGAT AGGCTGGTTC 
CCAGCTAATT ATGTAAAGCT TCTAAGCCCT GGGACGAGCA AAATCACTCC AACAGAGCCA 
CCTAAGTCAA CAGCATTAGC GGCAGTGTGC CAGGTGATTG GGATGTACGA CTACACCGCG 
CAGAATGACG ATGAGCTGGC CTTCAACAAG GGCCAGATCA TCAACGTCCT CAACAAGGAG 
GACCCTGACT GGTGGAAAGG AGAAGTCAAT GGACAAGTGG GGCTCTTCCC ATCCAATTAT 
GTGAAGCTGA CCACAGACAT GGACCCAAGC CAGCAATGGT GTTCAGACTT ACATCTCTTG 
GATATGTTGA CCCCAACTGA AAGAAAGCGA CAAGGATACA TCCACGAGCT CATTGTCACC 
GAGGAGAACT ATGTGAATGA CCTGCAGCTG GTCACAGAGA TTTTTCAAAA ACCCCTGATG 
GAGTCTGAGC TGCTGACAGA AAAAGAGGTT GCTATGATTT TTGTGAACTG GAAGGAGCTG 
ATTATGTGTA ATATCAAACT ACTAAAAGCG CTGAGAGTCC GCAAGAAGAT GTCCGGGGAG 
AAGATGCCTG TGAAGATGAT TGGAGACATC CTGAGCGCAC AGCTGCCGCA CATGCAGCCC 
TACATCCGCT TCTGCAGCCG CCAGCTCAAC GGGGCTGCCC TGATCCAGCA GAAGACGGAT 
GAGGCCCCAG ACTTCAAGGA GTTCGTCAAA AGATTGGCAA TGGATCCTCG GTGTAAAGGG 
ATGCCACTCT CTAGTTTTAT ACTGAAGCCT ATGCAACGGG TAACAAGATA CCCACTGATC 
ATTAAAAATA TCCTGGAAAA CACCCCTGAA AACCACCCGG ACCACAGCCA CTTGAAGCAC 
GCCCTGGAGA AGGCGGAAGA GCTCTGTTCC CAGGTGAACG AAGGGGTGCG GGAGAAGGAG 
AACTCTGACC GGCTGGAGTG GATCCAGGCC CACGTGCAGT GTGAAGGCCT GTCTGAGCAA 
CTTGTGTTCA ATTCAGTGAC CAATTGCTTG GGGCCGCGCA AATTTCTGCA CAGTGGGAAG 
CTCTACAAGG CCAAGAGCAA CAAGGAGCTG TATGGCTTCC TTTTCAACGA CTTCCTCCTG 
CTGACTCAGA TCACGAAGCC TTTGGGGTCT TCTGGCACCG ACAAAGTCTT CAGCCCCAAA 
TCAAACCTGC AGTATAAAAT GTATAAAACA CCTATTTTCC TAAATGAGGT TCTAGTAAAA 
TTACCCACCG ACCCTTCTGG AGACGAGCCC ATCTTCCACA TCTCCCACAT TGACCGCGTC 
TATACTCTCC GAGCAGAAAG CATAAATGAA AGGACTGCCT GGGTGCAGAA AATCAAAGCT 
GCTTCTGAAC TCTACATAGA GACTGAGAAA AAGAAGCGCG AGAAAGCGTA CCTGGTCCGT 
TCCCAAAGGG CAACAGGCAT TGGAAGGTTG ATGGTGAACG TGGTTGAAGG CATCGAGTTG 
AAACCCTGTC GGTCACATGG AAAGAGCAAC CCGTACTGTG AGGTGACCAT GGGTTCCCAG 
TGCCACATCA CCAAGACGAT CCAGGACACT CTGAACCCCA AGTGGAATTC CAACTGCCAG 
TTCTTCATCC GAGACCTGGA GCAGGAAGTC CTCTGCATCA CTGTGTTCGA GAGGGACCAG 
TTCTCACCAG ATGATTTTTT GGGTCGGACG GAGATCCGTG TGGCGGACAT CAAGAAAGAC 
CAGGGCTCCA AAGGTCCAGT TACGAAGTGT CTTCTGCTGC ACGAAGTCCC CACGGGAGAG 
ATTGTGGTCC GCTTGGACCT GCAGTTGTTT GATGAGCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883924.1
CDS	295..5136
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028435.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028435.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTTAAAG GGGAATGGGT GGATGAAAGC 
CAAACTGGAG AACCCGGCTG GCTTGGAGGA GAATTAAAAG GAAAGACAGG GTGGTTCCCT 
GCAAACTATG CAGAGAAAAT CCCAGAAAAT GAGGTTCCCG CTCCAGTGAA ACCAGTGACT 
GATTCAACAT CTGCCCCTGC CCCCAAACTG GCCTTGCGTG AGACCCCCGC CCCTTTGGCA 
GTAACCTCTT CAGAGCCCTC CACGACCCCT AATAACTGGG CCGACTTCAG CTCCACGTGG 
CCCACCAGCA CGAATGAGAA ACCAGAAACG GATAACTGGG ATGCATGGGC AGCCCAGCCC 
TCTCTCACCG TTCCAAGTGC CGGCCAGTTA AGGCAGAGGT CCGCCTTTAC TCCAGCCACG 
GCCACTGGCT CCTCCCCGTC TCCTGTGCTA GGCCAGGGTG AAAAGGTGGA GGGGCTACAA 
GCTCAAGCCC TATATCCTTG GAGAGCCAAA AAAGACAACC ACTTAAATTT TAACAAAAAT 
GATGTCATCA CCGTCCTGGA ACAGCAAGAC ATGTGGTGGT TTGGAGAAGT TCAAGGTCAG 
AAGGGTTGGT TCCCCAAGTC TTACGTGAAA CTCATTTCAG GGCCCATAAG GAAGTCTACA 
AGCATGGATT CTGGTTCTTC AGAGAGTCCT GCTAGTCTAA AGCGAGTAGC CTCTCCAGCA 
GCCAAGCCGG TCGTTTCGGG AGAAGAAATT GCCCAGGTTA TTGCCTCATA CACCGCCACC 
GGCCCCGAGC AGCTCACTCT CGCCCCTGGT CAGCTGATTT TGATCCGAAA AAAGAACCCA 
GGTGGATGGT GGGAAGGAGA GCTGCAAGCA CGTGGGAAAA AGCGCCAGAT AGGCTGGTTC 
CCAGCTAATT ATGTAAAGCT TCTAAGCCCT GGGACGAGCA AAATCACTCC AACAGAGCCA 
CCTAAGTCAA CAGCATTAGC GGCAGTGTGC CAGGTGATTG GGATGTACGA CTACACCGCG 
CAGAATGACG ATGAGCTGGC CTTCAACAAG GGCCAGATCA TCAACGTCCT CAACAAGGAG 
GACCCTGACT GGTGGAAAGG AGAAGTCAAT GGACAAGTGG GGCTCTTCCC ATCCAATTAT 
GTGAAGCTGA CCACAGACAT GGACCCAAGC CAGCAATGGT GTTCAGACTT ACATCTCTTG 
GATATGTTGA CCCCAACTGA AAGAAAGCGA CAAGGATACA TCCACGAGCT CATTGTCACC 
GAGGAGAACT ATGTGAATGA CCTGCAGCTG GTCACAGAGA TTTTTCAAAA ACCCCTGATG 
GAGTCTGAGC TGCTGACAGA AAAAGAGGTT GCTATGATTT TTGTGAACTG GAAGGAGCTG 
ATTATGTGTA ATATCAAACT ACTAAAAGCG CTGAGAGTCC GCAAGAAGAT GTCCGGGGAG 
AAGATGCCTG TGAAGATGAT TGGAGACATC CTGAGCGCAC AGCTGCCGCA CATGCAGCCC 
TACATCCGCT TCTGCAGCCG CCAGCTCAAC GGGGCTGCCC TGATCCAGCA GAAGACGGAT 
GAGGCCCCAG ACTTCAAGGA GTTCGTCAAA AGATTGGCAA TGGATCCTCG GTGTAAAGGG 
ATGCCACTCT CTAGTTTTAT ACTGAAGCCT ATGCAACGGG TAACAAGATA CCCACTGATC 
ATTAAAAATA TCCTGGAAAA CACCCCTGAA AACCACCCGG ACCACAGCCA CTTGAAGCAC 
GCCCTGGAGA AGGCGGAAGA GCTCTGTTCC CAGGTGAACG AAGGGGTGCG GGAGAAGGAG 
AACTCTGACC GGCTGGAGTG GATCCAGGCC CACGTGCAGT GTGAAGGCCT GTCTGAGCAA 
CTTGTGTTCA ATTCAGTGAC CAATTGCTTG GGGCCGCGCA AATTTCTGCA CAGTGGGAAG 
CTCTACAAGG CCAAGAGCAA CAAGGAGCTG TATGGCTTCC TTTTCAACGA CTTCCTCCTG 
CTGACTCAGA TCACGAAGCC TTTGGGGTCT TCTGGCACCG ACAAAGTCTT CAGCCCCAAA 
TCAAACCTGC AGTATAAAAT GTATAAAACA CCTATTTTCC TAAATGAGGT TCTAGTAAAA 
TTACCCACCG ACCCTTCTGG AGACGAGCCC ATCTTCCACA TCTCCCACAT TGACCGCGTC 
TATACTCTCC GAGCAGAAAG CATAAATGAA AGGACTGCCT GGGTGCAGAA AATCAAAGCT 
GCTTCTGAAC TCTACATAGA GACTGAGAAA AAGAAGCGCG AGAAAGCGTA CCTGGTCCGT 
TCCCAAAGGG CAACAGGCAT TGGAAGGTTG ATGGTGAACG TGGTTGAAGG CATCGAGTTG 
AAACCCTGTC GGTCACATGG AAAGAGCAAC CCGTACTGTG AGGTGACCAT GGGTTCCCAG 
TGCCACATCA CCAAGACGAT CCAGGACACT CTGAACCCCA AGTGGAATTC CAACTGCCAG 
TTCTTCATCC GAGACCTGGA GCAGGAAGTC CTCTGCATCA CTGTGTTCGA GAGGGACCAG 
TTCTCACCAG ATGATTTTTT GGGTCGGACG GAGATCCGTG TGGCGGACAT CAAGAAAGAC 
CAGGGCTCCA AAGGTCCAGT TACGAAGTGT CTTCTGCTGC ACGAAGTCCC CACGGGAGAG 
ATTGTGGTCC GCTTGGACCT GCAGTTGTTT GATGAGCCGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028436.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883925.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OHu93300
Clone ID Related Accession (Same CDS sequence)	XM_017028436.2 , XM_017028436.1
Accession Version	XM_017028436.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017028436.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AAATTGCCCA GGTTATTGCC TCATACACCG CCACCGGCCC CGAGCAGCTC 
ACTCTCGCCC CTGGTCAGCT GATTTTGATC CGAAAAAAGA ACCCAGGTGG ATGGTGGGAA 
GGAGAGCTGC AAGCACGTGG GAAAAAGCGC CAGATAGGCT GGTTCCCAGC TAATTATGTA 
AAGCTTCTAA GCCCTGGGAC GAGCAAAATC ACTCCAACAG AGCCACCTAA GTCAACAGCA 
TTAGCGGCAG TGTGCCAGGT GATTGGGATG TACGACTACA CCGCGCAGAA TGACGATGAG 
CTGGCCTTCA ACAAGGGCCA GATCATCAAC GTCCTCAACA AGGAGGACCC TGACTGGTGG 
AAAGGAGAAG TCAATGGACA AGTGGGGCTC TTCCCATCCA ATTATGTGAA GCTGACCACA 
GACATGGACC CAAGCCAGCA ATGGTGTTCA GACTTACATC TCTTGGATAT GTTGACCCCA 
ACTGAAAGAA AGCGACAAGG ATACATCCAC GAGCTCATTG TCACCGAGGA GAACTATGTG 
AATGACCTGC AGCTGGTCAC AGAGATTTTT CAAAAACCCC TGATGGAGTC TGAGCTGCTG 
ACAGAAAAAG AGGTTGCTAT GATTTTTGTG AACTGGAAGG AGCTGATTAT GTGTAATATC 
AAACTACTAA AAGCGCTGAG AGTCCGCAAG AAGATGTCCG GGGAGAAGAT GCCTGTGAAG 
ATGATTGGAG ACATCCTGAG CGCACAGCTG CCGCACATGC AGCCCTACAT CCGCTTCTGC 
AGCCGCCAGC TCAACGGGGC TGCCCTGATC CAGCAGAAGA CGGATGAGGC CCCAGACTTC 
AAGGAGTTCG TCAAAAGATT GGCAATGGAT CCTCGGTGTA AAGGGATGCC ACTCTCTAGT 
TTTATACTGA AGCCTATGCA ACGGGTAACA AGATACCCAC TGATCATTAA AAATATCCTG 
GAAAACACCC CTGAAAACCA CCCGGACCAC AGCCACTTGA AGCACGCCCT GGAGAAGGCG 
GAAGAGCTCT GTTCCCAGGT GAACGAAGGG GTGCGGGAGA AGGAGAACTC TGACCGGCTG 
GAGTGGATCC AGGCCCACGT GCAGTGTGAA GGCCTGTCTG AGCAACTTGT GTTCAATTCA 
GTGACCAATT GCTTGGGGCC GCGCAAATTT CTGCACAGTG GGAAGCTCTA CAAGGCCAAG 
AGCAACAAGG AGCTGTATGG CTTCCTTTTC AACGACTTCC TCCTGCTGAC TCAGATCACG 
AAGCCTTTGG GGTCTTCTGG CACCGACAAA GTCTTCAGCC CCAAATCAAA CCTGCAGTAT 
AAAATGTATA AAACACCTAT TTTCCTAAAT GAGGTTCTAG TAAAATTACC CACCGACCCT 
TCTGGAGACG AGCCCATCTT CCACATCTCC CACATTGACC GCGTCTATAC TCTCCGAGCA 
GAAAGCATAA ATGAAAGGAC TGCCTGGGTG CAGAAAATCA AAGCTGCTTC TGAACTCTAC 
ATAGAGACTG AGAAAAAGAA GCGCGAGAAA GCGTACCTGG TCCGTTCCCA AAGGGCAACA 
GGCATTGGAA GGTTGATGGT GAACGTGGTT GAAGGCATCG AGTTGAAACC CTGTCGGTCA 
CATGGAAAGA GCAACCCGTA CTGTGAGGTG ACCATGGGTT CCCAGTGCCA CATCACCAAG 
ACGATCCAGG ACACTCTGAA CCCCAAGTGG AATTCCAACT GCCAGTTCTT CATCCGAGAC 
CTGGAGCAGG AAGTCCTCTG CATCACTGTG TTCGAGAGGG ACCAGTTCTC ACCAGATGAT 
TTTTTGGGTC GGACGGAGAT CCGTGTGGCG GACATCAAGA AAGACCAGGG CTCCAAAGGT 
CCAGTTACGA AGTGTCTTCT GCTGCACGAA GTCCCCACGG GAGAGATTGT GGTCCGCTTG 
GACCTGCAGT TGTTTGATGA GCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883925.1
CDS	295..5121
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028436.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028436.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AAATTGCCCA GGTTATTGCC TCATACACCG CCACCGGCCC CGAGCAGCTC 
ACTCTCGCCC CTGGTCAGCT GATTTTGATC CGAAAAAAGA ACCCAGGTGG ATGGTGGGAA 
GGAGAGCTGC AAGCACGTGG GAAAAAGCGC CAGATAGGCT GGTTCCCAGC TAATTATGTA 
AAGCTTCTAA GCCCTGGGAC GAGCAAAATC ACTCCAACAG AGCCACCTAA GTCAACAGCA 
TTAGCGGCAG TGTGCCAGGT GATTGGGATG TACGACTACA CCGCGCAGAA TGACGATGAG 
CTGGCCTTCA ACAAGGGCCA GATCATCAAC GTCCTCAACA AGGAGGACCC TGACTGGTGG 
AAAGGAGAAG TCAATGGACA AGTGGGGCTC TTCCCATCCA ATTATGTGAA GCTGACCACA 
GACATGGACC CAAGCCAGCA ATGGTGTTCA GACTTACATC TCTTGGATAT GTTGACCCCA 
ACTGAAAGAA AGCGACAAGG ATACATCCAC GAGCTCATTG TCACCGAGGA GAACTATGTG 
AATGACCTGC AGCTGGTCAC AGAGATTTTT CAAAAACCCC TGATGGAGTC TGAGCTGCTG 
ACAGAAAAAG AGGTTGCTAT GATTTTTGTG AACTGGAAGG AGCTGATTAT GTGTAATATC 
AAACTACTAA AAGCGCTGAG AGTCCGCAAG AAGATGTCCG GGGAGAAGAT GCCTGTGAAG 
ATGATTGGAG ACATCCTGAG CGCACAGCTG CCGCACATGC AGCCCTACAT CCGCTTCTGC 
AGCCGCCAGC TCAACGGGGC TGCCCTGATC CAGCAGAAGA CGGATGAGGC CCCAGACTTC 
AAGGAGTTCG TCAAAAGATT GGCAATGGAT CCTCGGTGTA AAGGGATGCC ACTCTCTAGT 
TTTATACTGA AGCCTATGCA ACGGGTAACA AGATACCCAC TGATCATTAA AAATATCCTG 
GAAAACACCC CTGAAAACCA CCCGGACCAC AGCCACTTGA AGCACGCCCT GGAGAAGGCG 
GAAGAGCTCT GTTCCCAGGT GAACGAAGGG GTGCGGGAGA AGGAGAACTC TGACCGGCTG 
GAGTGGATCC AGGCCCACGT GCAGTGTGAA GGCCTGTCTG AGCAACTTGT GTTCAATTCA 
GTGACCAATT GCTTGGGGCC GCGCAAATTT CTGCACAGTG GGAAGCTCTA CAAGGCCAAG 
AGCAACAAGG AGCTGTATGG CTTCCTTTTC AACGACTTCC TCCTGCTGAC TCAGATCACG 
AAGCCTTTGG GGTCTTCTGG CACCGACAAA GTCTTCAGCC CCAAATCAAA CCTGCAGTAT 
AAAATGTATA AAACACCTAT TTTCCTAAAT GAGGTTCTAG TAAAATTACC CACCGACCCT 
TCTGGAGACG AGCCCATCTT CCACATCTCC CACATTGACC GCGTCTATAC TCTCCGAGCA 
GAAAGCATAA ATGAAAGGAC TGCCTGGGTG CAGAAAATCA AAGCTGCTTC TGAACTCTAC 
ATAGAGACTG AGAAAAAGAA GCGCGAGAAA GCGTACCTGG TCCGTTCCCA AAGGGCAACA 
GGCATTGGAA GGTTGATGGT GAACGTGGTT GAAGGCATCG AGTTGAAACC CTGTCGGTCA 
CATGGAAAGA GCAACCCGTA CTGTGAGGTG ACCATGGGTT CCCAGTGCCA CATCACCAAG 
ACGATCCAGG ACACTCTGAA CCCCAAGTGG AATTCCAACT GCCAGTTCTT CATCCGAGAC 
CTGGAGCAGG AAGTCCTCTG CATCACTGTG TTCGAGAGGG ACCAGTTCTC ACCAGATGAT 
TTTTTGGGTC GGACGGAGAT CCGTGTGGCG GACATCAAGA AAGACCAGGG CTCCAAAGGT 
CCAGTTACGA AGTGTCTTCT GCTGCACGAA GTCCCCACGG GAGAGATTGT GGTCCGCTTG 
GACCTGCAGT TGTTTGATGA GCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028436.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883925.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X10, mRNA.

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CloneID	OHu93300
Clone ID Related Accession (Same CDS sequence)	XM_017028436.2 , XM_017028436.1
Accession Version	XM_017028436.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AAATTGCCCA GGTTATTGCC TCATACACCG CCACCGGCCC CGAGCAGCTC 
ACTCTCGCCC CTGGTCAGCT GATTTTGATC CGAAAAAAGA ACCCAGGTGG ATGGTGGGAA 
GGAGAGCTGC AAGCACGTGG GAAAAAGCGC CAGATAGGCT GGTTCCCAGC TAATTATGTA 
AAGCTTCTAA GCCCTGGGAC GAGCAAAATC ACTCCAACAG AGCCACCTAA GTCAACAGCA 
TTAGCGGCAG TGTGCCAGGT GATTGGGATG TACGACTACA CCGCGCAGAA TGACGATGAG 
CTGGCCTTCA ACAAGGGCCA GATCATCAAC GTCCTCAACA AGGAGGACCC TGACTGGTGG 
AAAGGAGAAG TCAATGGACA AGTGGGGCTC TTCCCATCCA ATTATGTGAA GCTGACCACA 
GACATGGACC CAAGCCAGCA ATGGTGTTCA GACTTACATC TCTTGGATAT GTTGACCCCA 
ACTGAAAGAA AGCGACAAGG ATACATCCAC GAGCTCATTG TCACCGAGGA GAACTATGTG 
AATGACCTGC AGCTGGTCAC AGAGATTTTT CAAAAACCCC TGATGGAGTC TGAGCTGCTG 
ACAGAAAAAG AGGTTGCTAT GATTTTTGTG AACTGGAAGG AGCTGATTAT GTGTAATATC 
AAACTACTAA AAGCGCTGAG AGTCCGCAAG AAGATGTCCG GGGAGAAGAT GCCTGTGAAG 
ATGATTGGAG ACATCCTGAG CGCACAGCTG CCGCACATGC AGCCCTACAT CCGCTTCTGC 
AGCCGCCAGC TCAACGGGGC TGCCCTGATC CAGCAGAAGA CGGATGAGGC CCCAGACTTC 
AAGGAGTTCG TCAAAAGATT GGCAATGGAT CCTCGGTGTA AAGGGATGCC ACTCTCTAGT 
TTTATACTGA AGCCTATGCA ACGGGTAACA AGATACCCAC TGATCATTAA AAATATCCTG 
GAAAACACCC CTGAAAACCA CCCGGACCAC AGCCACTTGA AGCACGCCCT GGAGAAGGCG 
GAAGAGCTCT GTTCCCAGGT GAACGAAGGG GTGCGGGAGA AGGAGAACTC TGACCGGCTG 
GAGTGGATCC AGGCCCACGT GCAGTGTGAA GGCCTGTCTG AGCAACTTGT GTTCAATTCA 
GTGACCAATT GCTTGGGGCC GCGCAAATTT CTGCACAGTG GGAAGCTCTA CAAGGCCAAG 
AGCAACAAGG AGCTGTATGG CTTCCTTTTC AACGACTTCC TCCTGCTGAC TCAGATCACG 
AAGCCTTTGG GGTCTTCTGG CACCGACAAA GTCTTCAGCC CCAAATCAAA CCTGCAGTAT 
AAAATGTATA AAACACCTAT TTTCCTAAAT GAGGTTCTAG TAAAATTACC CACCGACCCT 
TCTGGAGACG AGCCCATCTT CCACATCTCC CACATTGACC GCGTCTATAC TCTCCGAGCA 
GAAAGCATAA ATGAAAGGAC TGCCTGGGTG CAGAAAATCA AAGCTGCTTC TGAACTCTAC 
ATAGAGACTG AGAAAAAGAA GCGCGAGAAA GCGTACCTGG TCCGTTCCCA AAGGGCAACA 
GGCATTGGAA GGTTGATGGT GAACGTGGTT GAAGGCATCG AGTTGAAACC CTGTCGGTCA 
CATGGAAAGA GCAACCCGTA CTGTGAGGTG ACCATGGGTT CCCAGTGCCA CATCACCAAG 
ACGATCCAGG ACACTCTGAA CCCCAAGTGG AATTCCAACT GCCAGTTCTT CATCCGAGAC 
CTGGAGCAGG AAGTCCTCTG CATCACTGTG TTCGAGAGGG ACCAGTTCTC ACCAGATGAT 
TTTTTGGGTC GGACGGAGAT CCGTGTGGCG GACATCAAGA AAGACCAGGG CTCCAAAGGT 
CCAGTTACGA AGTGTCTTCT GCTGCACGAA GTCCCCACGG GAGAGATTGT GGTCCGCTTG 
GACCTGCAGT TGTTTGATGA GCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883925.1
CDS	347..5173
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028436.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028436.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGCAGC TGTGCCCCCC 
CTGGCTAACG GGGCTCCCCC TGTTATACAA CCTCTGCCTG CATTTGCTCA TCCTGCAGCC 
ACATTGCCAA AGAGTTCTTC CTTTAGTAGA TCTGGTCCAG GGTCACAACT AAACACTAAA 
TTACAAAAGG CACAGTCATT TGATGTGGCC AGTGTCCCAC CAGTGGCAGA GTGGGCTGTT 
CCTCAGTCAT CAAGACTGAA ATACAGGCAA TTATTCAATA GTCATGACAA AACTATGAGT 
GGACACTTAA CAGGTCCCCA AGCAAGAACT ATTCTTATGC AGTCAAGTTT ACCACAGGCT 
CAGCTGGCTT CAATATGGAA TCTTTCTGAC ATTGATCAAG ATGGAAAACT TACAGCAGAG 
GAATTTATCC TGGCAATGCA CCTCATTGAT GTAGCTATGT CTGGCCAACC ACTGCCACCT 
GTCCTGCCTC CAGAATACAT TCCACCTTCT TTTAGAAGAG TTCGATCTGG CAGTGGTATA 
TCTGTCATAA GCTCAACATC TGTAGATCAG AGGCTACCAG AGGAACCAGT TTTAGAAGAT 
GAACAACAAC AATTAGAAAA GAAATTACCT GTAACGTTTG AAGATAAGAA GCGGGAGAAC 
TTTGAACGTG GCAACCTGGA ACTGGAGAAA CGAAGGCAAG CTCTCCTGGA ACAGCAGCGC 
AAGGAGCAGG AGCGCCTGGC CCAGCTGGAG CGGGCGGAGC AGGAGAGGAA GGAGCGTGAG 
CGCCAGGAGC AAGAGCGCAA AAGACAACTG GAACTGGAGA AGCAACTGGA AAAGCAGCGG 
GAGCTAGAAC GGCAGAGAGA GGAGGAGAGG AGGAAAGAAA TTGAGAGGCG AGAGGCTGCA 
AAACGGGAAC TTGAAAGGCA ACGACAACTT GAGTGGGAAC GGAATCGAAG GCAAGAACTA 
CTAAATCAAA GAAACAAAGA ACAAGAGGAC ATAGTTGTAC TGAAAGCAAA GAAAAAGACT 
TTGGAATTTG AATTAGAAGC TCTAAATGAT AAAAAGCATC AACTAGAAGG GAAACTTCAA 
GATATCAGAT GTCGATTGAC CACCCAAAGG CAAGAAATTG AGAGCACAAA CAAATCTAGA 
GAGTTGAGAA TTGCCGAAAT CACCCATCTA CAGCAACAAT TACAGGAATC TCAGCAAATG 
CTTGGAAGAC TTATTCCAGA AAAACAGATA CTCAATGACC AATTAAAACA AGTTCAGCAG 
AACAGTTTGC ACAGAGATTC ACTTGTTACA CTTAAAAGAG CCTTAGAAGC AAAAGAACTA 
GCTCGGCAGC ACCTACGAGA CCAACTGGAT GAAGTGGAGA AAGAAACTAG ATCAAAACTA 
CAGGAGATTG ATATTTTCAA TAATCAGCTG AAGGAACTAA GAGAAATACA CAATAAGCAA 
CAACTCCAGA AGCAAAAGTC CATGGAGGCT GAACGACTGA AACAGAAAGA ACAAGAACGA 
AAGATCATAG AATTAGAAAA ACAAAAAGAA GAAGCCCAAA GACGAGCTCA GGAAAGGGAC 
AAGCAGTGGC TGGAGCATGT GCAGCAGGAG GACGAGCATC AGAGACCAAG AAAACTCCAC 
GAAGAGGAAA AACTGAAAAG GGAGGAGAGT GTCAAAAAGA AGGATGGCGA GGAAAAAGGC 
AAACAGGAAG CACAAGACAA GCTGGGTCGG CTTTTCCATC AACACCAAGA ACCAGCTAAG 
CCAGCTGTCC AGGCACCCTG GTCCACTGCA GAAAAAGGTC CACTTACCAT TTCTGCACAG 
GAAAATGTAA AAGTGGTGTA TTACCGGGCA CTGTACCCCT TTGAATCCAG AAGCCATGAT 
GAAATCACTA TCCAGCCAGG AGACATAGTC ATGGTGGATG AAAGCCAAAC TGGAGAACCC 
GGCTGGCTTG GAGGAGAATT AAAAGGAAAG ACAGGGTGGT TCCCTGCAAA CTATGCAGAG 
AAAATCCCAG AAAATGAGGT TCCCGCTCCA GTGAAACCAG TGACTGATTC AACATCTGCC 
CCTGCCCCCA AACTGGCCTT GCGTGAGACC CCCGCCCCTT TGGCAGTAAC CTCTTCAGAG 
CCCTCCACGA CCCCTAATAA CTGGGCCGAC TTCAGCTCCA CGTGGCCCAC CAGCACGAAT 
GAGAAACCAG AAACGGATAA CTGGGATGCA TGGGCAGCCC AGCCCTCTCT CACCGTTCCA 
AGTGCCGGCC AGTTAAGGCA GAGGTCCGCC TTTACTCCAG CCACGGCCAC TGGCTCCTCC 
CCGTCTCCTG TGCTAGGCCA GGGTGAAAAG GTGGAGGGGC TACAAGCTCA AGCCCTATAT 
CCTTGGAGAG CCAAAAAAGA CAACCACTTA AATTTTAACA AAAATGATGT CATCACCGTC 
CTGGAACAGC AAGACATGTG GTGGTTTGGA GAAGTTCAAG GTCAGAAGGG TTGGTTCCCC 
AAGTCTTACG TGAAACTCAT TTCAGGGCCC ATAAGGAAGT CTACAAGCAT GGATTCTGGT 
TCTTCAGAGA GTCCTGCTAG TCTAAAGCGA GTAGCCTCTC CAGCAGCCAA GCCGGTCGTT 
TCGGGAGAAG AAATTGCCCA GGTTATTGCC TCATACACCG CCACCGGCCC CGAGCAGCTC 
ACTCTCGCCC CTGGTCAGCT GATTTTGATC CGAAAAAAGA ACCCAGGTGG ATGGTGGGAA 
GGAGAGCTGC AAGCACGTGG GAAAAAGCGC CAGATAGGCT GGTTCCCAGC TAATTATGTA 
AAGCTTCTAA GCCCTGGGAC GAGCAAAATC ACTCCAACAG AGCCACCTAA GTCAACAGCA 
TTAGCGGCAG TGTGCCAGGT GATTGGGATG TACGACTACA CCGCGCAGAA TGACGATGAG 
CTGGCCTTCA ACAAGGGCCA GATCATCAAC GTCCTCAACA AGGAGGACCC TGACTGGTGG 
AAAGGAGAAG TCAATGGACA AGTGGGGCTC TTCCCATCCA ATTATGTGAA GCTGACCACA 
GACATGGACC CAAGCCAGCA ATGGTGTTCA GACTTACATC TCTTGGATAT GTTGACCCCA 
ACTGAAAGAA AGCGACAAGG ATACATCCAC GAGCTCATTG TCACCGAGGA GAACTATGTG 
AATGACCTGC AGCTGGTCAC AGAGATTTTT CAAAAACCCC TGATGGAGTC TGAGCTGCTG 
ACAGAAAAAG AGGTTGCTAT GATTTTTGTG AACTGGAAGG AGCTGATTAT GTGTAATATC 
AAACTACTAA AAGCGCTGAG AGTCCGCAAG AAGATGTCCG GGGAGAAGAT GCCTGTGAAG 
ATGATTGGAG ACATCCTGAG CGCACAGCTG CCGCACATGC AGCCCTACAT CCGCTTCTGC 
AGCCGCCAGC TCAACGGGGC TGCCCTGATC CAGCAGAAGA CGGATGAGGC CCCAGACTTC 
AAGGAGTTCG TCAAAAGATT GGCAATGGAT CCTCGGTGTA AAGGGATGCC ACTCTCTAGT 
TTTATACTGA AGCCTATGCA ACGGGTAACA AGATACCCAC TGATCATTAA AAATATCCTG 
GAAAACACCC CTGAAAACCA CCCGGACCAC AGCCACTTGA AGCACGCCCT GGAGAAGGCG 
GAAGAGCTCT GTTCCCAGGT GAACGAAGGG GTGCGGGAGA AGGAGAACTC TGACCGGCTG 
GAGTGGATCC AGGCCCACGT GCAGTGTGAA GGCCTGTCTG AGCAACTTGT GTTCAATTCA 
GTGACCAATT GCTTGGGGCC GCGCAAATTT CTGCACAGTG GGAAGCTCTA CAAGGCCAAG 
AGCAACAAGG AGCTGTATGG CTTCCTTTTC AACGACTTCC TCCTGCTGAC TCAGATCACG 
AAGCCTTTGG GGTCTTCTGG CACCGACAAA GTCTTCAGCC CCAAATCAAA CCTGCAGTAT 
AAAATGTATA AAACACCTAT TTTCCTAAAT GAGGTTCTAG TAAAATTACC CACCGACCCT 
TCTGGAGACG AGCCCATCTT CCACATCTCC CACATTGACC GCGTCTATAC TCTCCGAGCA 
GAAAGCATAA ATGAAAGGAC TGCCTGGGTG CAGAAAATCA AAGCTGCTTC TGAACTCTAC 
ATAGAGACTG AGAAAAAGAA GCGCGAGAAA GCGTACCTGG TCCGTTCCCA AAGGGCAACA 
GGCATTGGAA GGTTGATGGT GAACGTGGTT GAAGGCATCG AGTTGAAACC CTGTCGGTCA 
CATGGAAAGA GCAACCCGTA CTGTGAGGTG ACCATGGGTT CCCAGTGCCA CATCACCAAG 
ACGATCCAGG ACACTCTGAA CCCCAAGTGG AATTCCAACT GCCAGTTCTT CATCCGAGAC 
CTGGAGCAGG AAGTCCTCTG CATCACTGTG TTCGAGAGGG ACCAGTTCTC ACCAGATGAT 
TTTTTGGGTC GGACGGAGAT CCGTGTGGCG GACATCAAGA AAGACCAGGG CTCCAAAGGT 
CCAGTTACGA AGTGTCTTCT GCTGCACGAA GTCCCCACGG GAGAGATTGT GGTCCGCTTG 
GACCTGCAGT TGTTTGATGA GCCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( NM_003024.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_003015.2 Homo sapiens intersectin 1 (ITSN1), transcript variant 1, mRNA.

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CloneID	OHu17805
Clone ID Related Accession (Same CDS sequence)	NM_003024.3 , XM_017028428.1 , NM_003024.2
Accession Version	NM_003024.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	intersectin-1 isoform ITSN-l
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058925.1, AF114487.1, AF064244.1 and AP000313.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_003024.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (ITSN-l). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF064244.1, AF114487.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000381318.8/ ENSP00000370719.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGGTGTTCAG ACTTACATCT CTTGGATATG TTGACCCCAA CTGAAAGAAA GCGACAAGGA 
TACATCCACG AGCTCATTGT CACCGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATTTTTC AAAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG 
ATTTTTGTGA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTAAA AGCGCTGAGA 
GTCCGCAAGA AGATGTCCGG GGAGAAGATG CCTGTGAAGA TGATTGGAGA CATCCTGAGC 
GCACAGCTGC CGCACATGCA GCCCTACATC CGCTTCTGCA GCCGCCAGCT CAACGGGGCT 
GCCCTGATCC AGCAGAAGAC GGATGAGGCC CCAGACTTCA AGGAGTTCGT CAAAAGATTG 
GCAATGGATC CTCGGTGTAA AGGGATGCCA CTCTCTAGTT TTATACTGAA GCCTATGCAA 
CGGGTAACAA GATACCCACT GATCATTAAA AATATCCTGG AAAACACCCC TGAAAACCAC 
CCGGACCACA GCCACTTGAA GCACGCCCTG GAGAAGGCGG AAGAGCTCTG TTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCT GACCGGCTGG AGTGGATCCA GGCCCACGTG 
CAGTGTGAAG GCCTGTCTGA GCAACTTGTG TTCAATTCAG TGACCAATTG CTTGGGGCCG 
CGCAAATTTC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTTTTCA ACGACTTCCT CCTGCTGACT CAGATCACGA AGCCTTTGGG GTCTTCTGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTATAA AACACCTATT 
TTCCTAAATG AGGTTCTAGT AAAATTACCC ACCGACCCTT CTGGAGACGA GCCCATCTTC 
CACATCTCCC ACATTGACCG CGTCTATACT CTCCGAGCAG AAAGCATAAA TGAAAGGACT 
GCCTGGGTGC AGAAAATCAA AGCTGCTTCT GAACTCTACA TAGAGACTGA GAAAAAGAAG 
CGCGAGAAAG CGTACCTGGT CCGTTCCCAA AGGGCAACAG GCATTGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATCGA GTTGAAACCC TGTCGGTCAC ATGGAAAGAG CAACCCGTAC 
TGTGAGGTGA CCATGGGTTC CCAGTGCCAC ATCACCAAGA CGATCCAGGA CACTCTGAAC 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCCGAGACC TGGAGCAGGA AGTCCTCTGC 
ATCACTGTGT TCGAGAGGGA CCAGTTCTCA CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGTGTGGCGG ACATCAAGAA AGACCAGGGC TCCAAAGGTC CAGTTACGAA GTGTCTTCTG 
CTGCACGAAG TCCCCACGGG AGAGATTGTG GTCCGCTTGG ACCTGCAGTT GTTTGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003015.2
CDS	246..5411
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	NM_003024.3
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003024.3

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGGTGTTCAG ACTTACATCT CTTGGATATG TTGACCCCAA CTGAAAGAAA GCGACAAGGA 
TACATCCACG AGCTCATTGT CACCGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATTTTTC AAAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG 
ATTTTTGTGA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTAAA AGCGCTGAGA 
GTCCGCAAGA AGATGTCCGG GGAGAAGATG CCTGTGAAGA TGATTGGAGA CATCCTGAGC 
GCACAGCTGC CGCACATGCA GCCCTACATC CGCTTCTGCA GCCGCCAGCT CAACGGGGCT 
GCCCTGATCC AGCAGAAGAC GGATGAGGCC CCAGACTTCA AGGAGTTCGT CAAAAGATTG 
GCAATGGATC CTCGGTGTAA AGGGATGCCA CTCTCTAGTT TTATACTGAA GCCTATGCAA 
CGGGTAACAA GATACCCACT GATCATTAAA AATATCCTGG AAAACACCCC TGAAAACCAC 
CCGGACCACA GCCACTTGAA GCACGCCCTG GAGAAGGCGG AAGAGCTCTG TTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCT GACCGGCTGG AGTGGATCCA GGCCCACGTG 
CAGTGTGAAG GCCTGTCTGA GCAACTTGTG TTCAATTCAG TGACCAATTG CTTGGGGCCG 
CGCAAATTTC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTTTTCA ACGACTTCCT CCTGCTGACT CAGATCACGA AGCCTTTGGG GTCTTCTGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTATAA AACACCTATT 
TTCCTAAATG AGGTTCTAGT AAAATTACCC ACCGACCCTT CTGGAGACGA GCCCATCTTC 
CACATCTCCC ACATTGACCG CGTCTATACT CTCCGAGCAG AAAGCATAAA TGAAAGGACT 
GCCTGGGTGC AGAAAATCAA AGCTGCTTCT GAACTCTACA TAGAGACTGA GAAAAAGAAG 
CGCGAGAAAG CGTACCTGGT CCGTTCCCAA AGGGCAACAG GCATTGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATCGA GTTGAAACCC TGTCGGTCAC ATGGAAAGAG CAACCCGTAC 
TGTGAGGTGA CCATGGGTTC CCAGTGCCAC ATCACCAAGA CGATCCAGGA CACTCTGAAC 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCCGAGACC TGGAGCAGGA AGTCCTCTGC 
ATCACTGTGT TCGAGAGGGA CCAGTTCTCA CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGTGTGGCGG ACATCAAGAA AGACCAGGGC TCCAAAGGTC CAGTTACGAA GTGTCTTCTG 
CTGCACGAAG TCCCCACGGG AGAGATTGTG GTCCGCTTGG ACCTGCAGTT GTTTGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( NM_003024.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_003015.2 Homo sapiens intersectin 1 (ITSN1), transcript variant 1, mRNA.

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CloneID	OHu17805
Clone ID Related Accession (Same CDS sequence)	NM_003024.3 , XM_017028428.1 , NM_003024.2
Accession Version	NM_003024.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	intersectin-1 isoform ITSN-l
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC058925.1, AF114487.1 and AF064244.1. This sequence is a reference standard in the RefSeqGene project. On May 27, 2004 this sequence version replaced NM_003024.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (ITSN-l). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF114487.1, AF064244.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145743, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGGTGTTCAG ACTTACATCT CTTGGATATG TTGACCCCAA CTGAAAGAAA GCGACAAGGA 
TACATCCACG AGCTCATTGT CACCGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATTTTTC AAAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG 
ATTTTTGTGA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTAAA AGCGCTGAGA 
GTCCGCAAGA AGATGTCCGG GGAGAAGATG CCTGTGAAGA TGATTGGAGA CATCCTGAGC 
GCACAGCTGC CGCACATGCA GCCCTACATC CGCTTCTGCA GCCGCCAGCT CAACGGGGCT 
GCCCTGATCC AGCAGAAGAC GGATGAGGCC CCAGACTTCA AGGAGTTCGT CAAAAGATTG 
GCAATGGATC CTCGGTGTAA AGGGATGCCA CTCTCTAGTT TTATACTGAA GCCTATGCAA 
CGGGTAACAA GATACCCACT GATCATTAAA AATATCCTGG AAAACACCCC TGAAAACCAC 
CCGGACCACA GCCACTTGAA GCACGCCCTG GAGAAGGCGG AAGAGCTCTG TTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCT GACCGGCTGG AGTGGATCCA GGCCCACGTG 
CAGTGTGAAG GCCTGTCTGA GCAACTTGTG TTCAATTCAG TGACCAATTG CTTGGGGCCG 
CGCAAATTTC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTTTTCA ACGACTTCCT CCTGCTGACT CAGATCACGA AGCCTTTGGG GTCTTCTGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTATAA AACACCTATT 
TTCCTAAATG AGGTTCTAGT AAAATTACCC ACCGACCCTT CTGGAGACGA GCCCATCTTC 
CACATCTCCC ACATTGACCG CGTCTATACT CTCCGAGCAG AAAGCATAAA TGAAAGGACT 
GCCTGGGTGC AGAAAATCAA AGCTGCTTCT GAACTCTACA TAGAGACTGA GAAAAAGAAG 
CGCGAGAAAG CGTACCTGGT CCGTTCCCAA AGGGCAACAG GCATTGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATCGA GTTGAAACCC TGTCGGTCAC ATGGAAAGAG CAACCCGTAC 
TGTGAGGTGA CCATGGGTTC CCAGTGCCAC ATCACCAAGA CGATCCAGGA CACTCTGAAC 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCCGAGACC TGGAGCAGGA AGTCCTCTGC 
ATCACTGTGT TCGAGAGGGA CCAGTTCTCA CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGTGTGGCGG ACATCAAGAA AGACCAGGGC TCCAAAGGTC CAGTTACGAA GTGTCTTCTG 
CTGCACGAAG TCCCCACGGG AGAGATTGTG GTCCGCTTGG ACCTGCAGTT GTTTGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_003015.2
CDS	269..5434
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	NM_003024.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003024.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGGTGTTCAG ACTTACATCT CTTGGATATG TTGACCCCAA CTGAAAGAAA GCGACAAGGA 
TACATCCACG AGCTCATTGT CACCGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATTTTTC AAAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG 
ATTTTTGTGA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTAAA AGCGCTGAGA 
GTCCGCAAGA AGATGTCCGG GGAGAAGATG CCTGTGAAGA TGATTGGAGA CATCCTGAGC 
GCACAGCTGC CGCACATGCA GCCCTACATC CGCTTCTGCA GCCGCCAGCT CAACGGGGCT 
GCCCTGATCC AGCAGAAGAC GGATGAGGCC CCAGACTTCA AGGAGTTCGT CAAAAGATTG 
GCAATGGATC CTCGGTGTAA AGGGATGCCA CTCTCTAGTT TTATACTGAA GCCTATGCAA 
CGGGTAACAA GATACCCACT GATCATTAAA AATATCCTGG AAAACACCCC TGAAAACCAC 
CCGGACCACA GCCACTTGAA GCACGCCCTG GAGAAGGCGG AAGAGCTCTG TTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCT GACCGGCTGG AGTGGATCCA GGCCCACGTG 
CAGTGTGAAG GCCTGTCTGA GCAACTTGTG TTCAATTCAG TGACCAATTG CTTGGGGCCG 
CGCAAATTTC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTTTTCA ACGACTTCCT CCTGCTGACT CAGATCACGA AGCCTTTGGG GTCTTCTGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTATAA AACACCTATT 
TTCCTAAATG AGGTTCTAGT AAAATTACCC ACCGACCCTT CTGGAGACGA GCCCATCTTC 
CACATCTCCC ACATTGACCG CGTCTATACT CTCCGAGCAG AAAGCATAAA TGAAAGGACT 
GCCTGGGTGC AGAAAATCAA AGCTGCTTCT GAACTCTACA TAGAGACTGA GAAAAAGAAG 
CGCGAGAAAG CGTACCTGGT CCGTTCCCAA AGGGCAACAG GCATTGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATCGA GTTGAAACCC TGTCGGTCAC ATGGAAAGAG CAACCCGTAC 
TGTGAGGTGA CCATGGGTTC CCAGTGCCAC ATCACCAAGA CGATCCAGGA CACTCTGAAC 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCCGAGACC TGGAGCAGGA AGTCCTCTGC 
ATCACTGTGT TCGAGAGGGA CCAGTTCTCA CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGTGTGGCGG ACATCAAGAA AGACCAGGGC TCCAAAGGTC CAGTTACGAA GTGTCTTCTG 
CTGCACGAAG TCCCCACGGG AGAGATTGTG GTCCGCTTGG ACCTGCAGTT GTTTGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( NM_001331010.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317939.1 Homo sapiens intersectin 1 (ITSN1), transcript variant 5, mRNA.

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CloneID	OHu53299
Clone ID Related Accession (Same CDS sequence)	NM_001331010.1 , NM_001331010.2
Accession Version	NM_001331010.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-25
Organism	Homo sapiens(human)
Product	intersectin-1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK027846.1, AP000308.1, AP000311.1, KF511399.1, AP000312.2 and AP000313.1. On Sep 3, 2016 this sequence version replaced XM_005261026.2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC116185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GAGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGGCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CCCAGGTTAT TGCCTCATAC 
ACCGCCACCG GCCCCGAGCA GCTCACTCTC GCCCCTGGTC AGCTGATTTT GATCCGAAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTGCAAGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTCC CAGCTAATTA TGTAAAGCTT CTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGAGCCAC CTAAGTCAAC AGCATTAGCG GCAGTGTGCC AGGTGATTGG GATGTACGAC 
TACACCGCGC AGAATGACGA TGAGCTGGCC TTCAACAAGG GCCAGATCAT CAACGTCCTC 
AACAAGGAGG ACCCTGACTG GTGGAAAGGA GAAGTCAATG GACAAGTGGG GCTCTTCCCA 
TCCAATTATG TGAAGCTGAC CACAGACATG GACCCAAGCC AGCAATGGTG TTCAGACTTA 
CATCTCTTGG ATATGTTGAC CCCAACTGAA AGAAAGCGAC AAGGATACAT CCACGAGCTC 
ATTGTCACCG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT TTTTCAAAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGTTG CTATGATTTT TGTGAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTAAAAGCGC TGAGAGTCCG CAAGAAGATG 
TCCGGGGAGA AGATGCCTGT GAAGATGATT GGAGACATCC TGAGCGCACA GCTGCCGCAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCCGC CAGCTCAACG GGGCTGCCCT GATCCAGCAG 
AAGACGGATG AGGCCCCAGA CTTCAAGGAG TTCGTCAAAA GATTGGCAAT GGATCCTCGG 
TGTAAAGGGA TGCCACTCTC TAGTTTTATA CTGAAGCCTA TGCAACGGGT AACAAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACCCCTGAAA ACCACCCGGA CCACAGCCAC 
TTGAAGCACG CCCTGGAGAA GGCGGAAGAG CTCTGTTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCTGACCG GCTGGAGTGG ATCCAGGCCC ACGTGCAGTG TGAAGGCCTG 
TCTGAGCAAC TTGTGTTCAA TTCAGTGACC AATTGCTTGG GGCCGCGCAA ATTTCTGCAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT TTTCAACGAC 
TTCCTCCTGC TGACTCAGAT CACGAAGCCT TTGGGGTCTT CTGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACCTGCA GTATAAAATG TATAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTAAAAT TACCCACCGA CCCTTCTGGA GACGAGCCCA TCTTCCACAT CTCCCACATT 
GACCGCGTCT ATACTCTCCG AGCAGAAAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCTG CTTCTGAACT CTACATAGAG ACTGAGAAAA AGAAGCGCGA GAAAGCGTAC 
CTGGTCCGTT CCCAAAGGGC AACAGGCATT GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATCGAGTTGA AACCCTGTCG GTCACATGGA AAGAGCAACC CGTACTGTGA GGTGACCATG 
GGTTCCCAGT GCCACATCAC CAAGACGATC CAGGACACTC TGAACCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCCG AGACCTGGAG CAGGAAGTCC TCTGCATCAC TGTGTTCGAG 
AGGGACCAGT TCTCACCAGA TGATTTTTTG GGTCGGACGG AGATCCGTGT GGCGGACATC 
AAGAAAGACC AGGGCTCCAA AGGTCCAGTT ACGAAGTGTC TTCTGCTGCA CGAAGTCCCC 
ACGGGAGAGA TTGTGGTCCG CTTGGACCTG CAGTTGTTTG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317939.1
CDS	347..5497
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	NM_001331010.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331010.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GAGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGGCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CCCAGGTTAT TGCCTCATAC 
ACCGCCACCG GCCCCGAGCA GCTCACTCTC GCCCCTGGTC AGCTGATTTT GATCCGAAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTGCAAGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTCC CAGCTAATTA TGTAAAGCTT CTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGAGCCAC CTAAGTCAAC AGCATTAGCG GCAGTGTGCC AGGTGATTGG GATGTACGAC 
TACACCGCGC AGAATGACGA TGAGCTGGCC TTCAACAAGG GCCAGATCAT CAACGTCCTC 
AACAAGGAGG ACCCTGACTG GTGGAAAGGA GAAGTCAATG GACAAGTGGG GCTCTTCCCA 
TCCAATTATG TGAAGCTGAC CACAGACATG GACCCAAGCC AGCAATGGTG TTCAGACTTA 
CATCTCTTGG ATATGTTGAC CCCAACTGAA AGAAAGCGAC AAGGATACAT CCACGAGCTC 
ATTGTCACCG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT TTTTCAAAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGTTG CTATGATTTT TGTGAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTAAAAGCGC TGAGAGTCCG CAAGAAGATG 
TCCGGGGAGA AGATGCCTGT GAAGATGATT GGAGACATCC TGAGCGCACA GCTGCCGCAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCCGC CAGCTCAACG GGGCTGCCCT GATCCAGCAG 
AAGACGGATG AGGCCCCAGA CTTCAAGGAG TTCGTCAAAA GATTGGCAAT GGATCCTCGG 
TGTAAAGGGA TGCCACTCTC TAGTTTTATA CTGAAGCCTA TGCAACGGGT AACAAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACCCCTGAAA ACCACCCGGA CCACAGCCAC 
TTGAAGCACG CCCTGGAGAA GGCGGAAGAG CTCTGTTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCTGACCG GCTGGAGTGG ATCCAGGCCC ACGTGCAGTG TGAAGGCCTG 
TCTGAGCAAC TTGTGTTCAA TTCAGTGACC AATTGCTTGG GGCCGCGCAA ATTTCTGCAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT TTTCAACGAC 
TTCCTCCTGC TGACTCAGAT CACGAAGCCT TTGGGGTCTT CTGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACCTGCA GTATAAAATG TATAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTAAAAT TACCCACCGA CCCTTCTGGA GACGAGCCCA TCTTCCACAT CTCCCACATT 
GACCGCGTCT ATACTCTCCG AGCAGAAAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCTG CTTCTGAACT CTACATAGAG ACTGAGAAAA AGAAGCGCGA GAAAGCGTAC 
CTGGTCCGTT CCCAAAGGGC AACAGGCATT GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATCGAGTTGA AACCCTGTCG GTCACATGGA AAGAGCAACC CGTACTGTGA GGTGACCATG 
GGTTCCCAGT GCCACATCAC CAAGACGATC CAGGACACTC TGAACCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCCG AGACCTGGAG CAGGAAGTCC TCTGCATCAC TGTGTTCGAG 
AGGGACCAGT TCTCACCAGA TGATTTTTTG GGTCGGACGG AGATCCGTGT GGCGGACATC 
AAGAAAGACC AGGGCTCCAA AGGTCCAGTT ACGAAGTGTC TTCTGCTGCA CGAAGTCCCC 
ACGGGAGAGA TTGTGGTCCG CTTGGACCTG CAGTTGTTTG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( NM_001331010.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001317939.1 Homo sapiens intersectin 1 (ITSN1), transcript variant 5, mRNA.

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CloneID	OHu53299
Clone ID Related Accession (Same CDS sequence)	NM_001331010.1 , NM_001331010.2
Accession Version	NM_001331010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5151bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	intersectin-1 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK027846.1, AP000308.1, AP000311.1, KF511399.1, AP000312.2 and AP000313.1. On May 31, 2019 this sequence version replaced NM_001331010.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC116185.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GAGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGGCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CCCAGGTTAT TGCCTCATAC 
ACCGCCACCG GCCCCGAGCA GCTCACTCTC GCCCCTGGTC AGCTGATTTT GATCCGAAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTGCAAGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTCC CAGCTAATTA TGTAAAGCTT CTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGAGCCAC CTAAGTCAAC AGCATTAGCG GCAGTGTGCC AGGTGATTGG GATGTACGAC 
TACACCGCGC AGAATGACGA TGAGCTGGCC TTCAACAAGG GCCAGATCAT CAACGTCCTC 
AACAAGGAGG ACCCTGACTG GTGGAAAGGA GAAGTCAATG GACAAGTGGG GCTCTTCCCA 
TCCAATTATG TGAAGCTGAC CACAGACATG GACCCAAGCC AGCAATGGTG TTCAGACTTA 
CATCTCTTGG ATATGTTGAC CCCAACTGAA AGAAAGCGAC AAGGATACAT CCACGAGCTC 
ATTGTCACCG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT TTTTCAAAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGTTG CTATGATTTT TGTGAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTAAAAGCGC TGAGAGTCCG CAAGAAGATG 
TCCGGGGAGA AGATGCCTGT GAAGATGATT GGAGACATCC TGAGCGCACA GCTGCCGCAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCCGC CAGCTCAACG GGGCTGCCCT GATCCAGCAG 
AAGACGGATG AGGCCCCAGA CTTCAAGGAG TTCGTCAAAA GATTGGCAAT GGATCCTCGG 
TGTAAAGGGA TGCCACTCTC TAGTTTTATA CTGAAGCCTA TGCAACGGGT AACAAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACCCCTGAAA ACCACCCGGA CCACAGCCAC 
TTGAAGCACG CCCTGGAGAA GGCGGAAGAG CTCTGTTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCTGACCG GCTGGAGTGG ATCCAGGCCC ACGTGCAGTG TGAAGGCCTG 
TCTGAGCAAC TTGTGTTCAA TTCAGTGACC AATTGCTTGG GGCCGCGCAA ATTTCTGCAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT TTTCAACGAC 
TTCCTCCTGC TGACTCAGAT CACGAAGCCT TTGGGGTCTT CTGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACCTGCA GTATAAAATG TATAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTAAAAT TACCCACCGA CCCTTCTGGA GACGAGCCCA TCTTCCACAT CTCCCACATT 
GACCGCGTCT ATACTCTCCG AGCAGAAAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCTG CTTCTGAACT CTACATAGAG ACTGAGAAAA AGAAGCGCGA GAAAGCGTAC 
CTGGTCCGTT CCCAAAGGGC AACAGGCATT GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATCGAGTTGA AACCCTGTCG GTCACATGGA AAGAGCAACC CGTACTGTGA GGTGACCATG 
GGTTCCCAGT GCCACATCAC CAAGACGATC CAGGACACTC TGAACCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCCG AGACCTGGAG CAGGAAGTCC TCTGCATCAC TGTGTTCGAG 
AGGGACCAGT TCTCACCAGA TGATTTTTTG GGTCGGACGG AGATCCGTGT GGCGGACATC 
AAGAAAGACC AGGGCTCCAA AGGTCCAGTT ACGAAGTGTC TTCTGCTGCA CGAAGTCCCC 
ACGGGAGAGA TTGTGGTCCG CTTGGACCTG CAGTTGTTTG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001317939.1
CDS	246..5396
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	NM_001331010.2
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001331010.2

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTGGAT GAAAGCCAAA CTGGAGAACC CGGCTGGCTT 
GGAGGAGAAT TAAAAGGAAA GACAGGGTGG TTCCCTGCAA ACTATGCAGA GAAAATCCCA 
GAAAATGAGG TTCCCGCTCC AGTGAAACCA GTGACTGATT CAACATCTGC CCCTGCCCCC 
AAACTGGCCT TGCGTGAGAC CCCCGCCCCT TTGGCAGTAA CCTCTTCAGA GCCCTCCACG 
ACCCCTAATA ACTGGGCCGA CTTCAGCTCC ACGTGGCCCA CCAGCACGAA TGAGAAACCA 
GAAACGGATA ACTGGGATGC ATGGGCAGCC CAGCCCTCTC TCACCGTTCC AAGTGCCGGC 
CAGTTAAGGC AGAGGTCCGC CTTTACTCCA GCCACGGCCA CTGGCTCCTC CCCGTCTCCT 
GTGCTAGGCC AGGGTGAAAA GGTGGAGGGG CTACAAGCTC AAGCCCTATA TCCTTGGAGA 
GCCAAAAAAG ACAACCACTT AAATTTTAAC AAAAATGATG TCATCACCGT CCTGGAACAG 
CAAGACATGT GGTGGTTTGG AGAAGTTCAA GGTCAGAAGG GTTGGTTCCC CAAGTCTTAC 
GTGAAACTCA TTTCAGGGCC CATAAGGAAG TCTACAAGCA TGGATTCTGG TTCTTCAGAG 
AGTCCTGCTA GTCTAAAGCG AGTAGCCTCT CCAGCAGCCA AGCCGGTCGT TTCGGGAGAA 
GAATTTATTG CCATGTACAC TTACGAGAGT TCTGAGCAAG GAGATTTAAC CTTTCAGCAA 
GGGGATGTGA TTTTGGTTAC CAAGAAAGAT GGTGACTGGT GGACAGGAAC AGTGGGCGAC 
AAGGCCGGAG TCTTCCCTTC TAACTATGTG AGGCTTAAAG ATTCAGAGGG CTCTGGAACT 
GCTGGGAAAA CAGGGAGTTT AGGAAAAAAA CCTGAAATTG CCCAGGTTAT TGCCTCATAC 
ACCGCCACCG GCCCCGAGCA GCTCACTCTC GCCCCTGGTC AGCTGATTTT GATCCGAAAA 
AAGAACCCAG GTGGATGGTG GGAAGGAGAG CTGCAAGCAC GTGGGAAAAA GCGCCAGATA 
GGCTGGTTCC CAGCTAATTA TGTAAAGCTT CTAAGCCCTG GGACGAGCAA AATCACTCCA 
ACAGAGCCAC CTAAGTCAAC AGCATTAGCG GCAGTGTGCC AGGTGATTGG GATGTACGAC 
TACACCGCGC AGAATGACGA TGAGCTGGCC TTCAACAAGG GCCAGATCAT CAACGTCCTC 
AACAAGGAGG ACCCTGACTG GTGGAAAGGA GAAGTCAATG GACAAGTGGG GCTCTTCCCA 
TCCAATTATG TGAAGCTGAC CACAGACATG GACCCAAGCC AGCAATGGTG TTCAGACTTA 
CATCTCTTGG ATATGTTGAC CCCAACTGAA AGAAAGCGAC AAGGATACAT CCACGAGCTC 
ATTGTCACCG AGGAGAACTA TGTGAATGAC CTGCAGCTGG TCACAGAGAT TTTTCAAAAA 
CCCCTGATGG AGTCTGAGCT GCTGACAGAA AAAGAGGTTG CTATGATTTT TGTGAACTGG 
AAGGAGCTGA TTATGTGTAA TATCAAACTA CTAAAAGCGC TGAGAGTCCG CAAGAAGATG 
TCCGGGGAGA AGATGCCTGT GAAGATGATT GGAGACATCC TGAGCGCACA GCTGCCGCAC 
ATGCAGCCCT ACATCCGCTT CTGCAGCCGC CAGCTCAACG GGGCTGCCCT GATCCAGCAG 
AAGACGGATG AGGCCCCAGA CTTCAAGGAG TTCGTCAAAA GATTGGCAAT GGATCCTCGG 
TGTAAAGGGA TGCCACTCTC TAGTTTTATA CTGAAGCCTA TGCAACGGGT AACAAGATAC 
CCACTGATCA TTAAAAATAT CCTGGAAAAC ACCCCTGAAA ACCACCCGGA CCACAGCCAC 
TTGAAGCACG CCCTGGAGAA GGCGGAAGAG CTCTGTTCCC AGGTGAACGA AGGGGTGCGG 
GAGAAGGAGA ACTCTGACCG GCTGGAGTGG ATCCAGGCCC ACGTGCAGTG TGAAGGCCTG 
TCTGAGCAAC TTGTGTTCAA TTCAGTGACC AATTGCTTGG GGCCGCGCAA ATTTCTGCAC 
AGTGGGAAGC TCTACAAGGC CAAGAGCAAC AAGGAGCTGT ATGGCTTCCT TTTCAACGAC 
TTCCTCCTGC TGACTCAGAT CACGAAGCCT TTGGGGTCTT CTGGCACCGA CAAAGTCTTC 
AGCCCCAAAT CAAACCTGCA GTATAAAATG TATAAAACAC CTATTTTCCT AAATGAGGTT 
CTAGTAAAAT TACCCACCGA CCCTTCTGGA GACGAGCCCA TCTTCCACAT CTCCCACATT 
GACCGCGTCT ATACTCTCCG AGCAGAAAGC ATAAATGAAA GGACTGCCTG GGTGCAGAAA 
ATCAAAGCTG CTTCTGAACT CTACATAGAG ACTGAGAAAA AGAAGCGCGA GAAAGCGTAC 
CTGGTCCGTT CCCAAAGGGC AACAGGCATT GGAAGGTTGA TGGTGAACGT GGTTGAAGGC 
ATCGAGTTGA AACCCTGTCG GTCACATGGA AAGAGCAACC CGTACTGTGA GGTGACCATG 
GGTTCCCAGT GCCACATCAC CAAGACGATC CAGGACACTC TGAACCCCAA GTGGAATTCC 
AACTGCCAGT TCTTCATCCG AGACCTGGAG CAGGAAGTCC TCTGCATCAC TGTGTTCGAG 
AGGGACCAGT TCTCACCAGA TGATTTTTTG GGTCGGACGG AGATCCGTGT GGCGGACATC 
AAGAAAGACC AGGGCTCCAA AGGTCCAGTT ACGAAGTGTC TTCTGCTGCA CGAAGTCCCC 
ACGGGAGAGA TTGTGGTCCG CTTGGACCTG CAGTTGTTTG ATGAGCCGTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ITSN1 ( XM_017028428.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883917.1 Homo sapiens intersectin 1 (ITSN1), transcript variant X1, mRNA.

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CloneID	OHu17805
Clone ID Related Accession (Same CDS sequence)	NM_003024.3 , XM_017028428.1 , NM_003024.2
Accession Version	XM_017028428.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5166bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	intersectin-1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000021.9) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGGTGTTCAG ACTTACATCT CTTGGATATG TTGACCCCAA CTGAAAGAAA GCGACAAGGA 
TACATCCACG AGCTCATTGT CACCGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATTTTTC AAAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG 
ATTTTTGTGA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTAAA AGCGCTGAGA 
GTCCGCAAGA AGATGTCCGG GGAGAAGATG CCTGTGAAGA TGATTGGAGA CATCCTGAGC 
GCACAGCTGC CGCACATGCA GCCCTACATC CGCTTCTGCA GCCGCCAGCT CAACGGGGCT 
GCCCTGATCC AGCAGAAGAC GGATGAGGCC CCAGACTTCA AGGAGTTCGT CAAAAGATTG 
GCAATGGATC CTCGGTGTAA AGGGATGCCA CTCTCTAGTT TTATACTGAA GCCTATGCAA 
CGGGTAACAA GATACCCACT GATCATTAAA AATATCCTGG AAAACACCCC TGAAAACCAC 
CCGGACCACA GCCACTTGAA GCACGCCCTG GAGAAGGCGG AAGAGCTCTG TTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCT GACCGGCTGG AGTGGATCCA GGCCCACGTG 
CAGTGTGAAG GCCTGTCTGA GCAACTTGTG TTCAATTCAG TGACCAATTG CTTGGGGCCG 
CGCAAATTTC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTTTTCA ACGACTTCCT CCTGCTGACT CAGATCACGA AGCCTTTGGG GTCTTCTGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTATAA AACACCTATT 
TTCCTAAATG AGGTTCTAGT AAAATTACCC ACCGACCCTT CTGGAGACGA GCCCATCTTC 
CACATCTCCC ACATTGACCG CGTCTATACT CTCCGAGCAG AAAGCATAAA TGAAAGGACT 
GCCTGGGTGC AGAAAATCAA AGCTGCTTCT GAACTCTACA TAGAGACTGA GAAAAAGAAG 
CGCGAGAAAG CGTACCTGGT CCGTTCCCAA AGGGCAACAG GCATTGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATCGA GTTGAAACCC TGTCGGTCAC ATGGAAAGAG CAACCCGTAC 
TGTGAGGTGA CCATGGGTTC CCAGTGCCAC ATCACCAAGA CGATCCAGGA CACTCTGAAC 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCCGAGACC TGGAGCAGGA AGTCCTCTGC 
ATCACTGTGT TCGAGAGGGA CCAGTTCTCA CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGTGTGGCGG ACATCAAGAA AGACCAGGGC TCCAAAGGTC CAGTTACGAA GTGTCTTCTG 
CTGCACGAAG TCCCCACGGG AGAGATTGTG GTCCGCTTGG ACCTGCAGTT GTTTGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883917.1
CDS	267..5432
Translation	MAQFPTPFGGSLDIWAITVEERAKHDQQFHSLKPISGFITGDQARNFFFQSGLPQPVLAQIWALADMNNDGRMDQVEFSIAMKLIKLKLQGYQLPSALPPVMKQQPVAISSAPAFGMGGIASMPPLTAVAPVPMGSIPVVGMSPTLVSSVPTAAVPPLANGAPPVIQPLPAFAHPAATLPKSSSFSRSGPGSQLNTKLQKAQSFDVASVPPVAEWAVPQSSRLKYRQLFNSHDKTMSGHLTGPQARTILMQSSLPQAQLASIWNLSDIDQDGKLTAEEFILAMHLIDVAMSGQPLPPVLPPEYIPPSFRRVRSGSGISVISSTSVDQRLPEEPVLEDEQQQLEKKLPVTFEDKKRENFERGNLELEKRRQALLEQQRKEQERLAQLERAEQERKERERQEQERKRQLELEKQLEKQRELERQREEERRKEIERREAAKRELERQRQLEWERNRRQELLNQRNKEQEDIVVLKAKKKTLEFELEALNDKKHQLEGKLQDIRCRLTTQRQEIESTNKSRELRIAEITHLQQQLQESQQMLGRLIPEKQILNDQLKQVQQNSLHRDSLVTLKRALEAKELARQHLRDQLDEVEKETRSKLQEIDIFNNQLKELREIHNKQQLQKQKSMEAERLKQKEQERKIIELEKQKEEAQRRAQERDKQWLEHVQQEDEHQRPRKLHEEEKLKREESVKKKDGEEKGKQEAQDKLGRLFHQHQEPAKPAVQAPWSTAEKGPLTISAQENVKVVYYRALYPFESRSHDEITIQPGDIVMVKGEWVDESQTGEPGWLGGELKGKTGWFPANYAEKIPENEVPAPVKPVTDSTSAPAPKLALRETPAPLAVTSSEPSTTPNNWADFSSTWPTSTNEKPETDNWDAWAAQPSLTVPSAGQLRQRSAFTPATATGSSPSPVLGQGEKVEGLQAQALYPWRAKKDNHLNFNKNDVITVLEQQDMWWFGEVQGQKGWFPKSYVKLISGPIRKSTSMDSGSSESPASLKRVASPAAKPVVSGEEFIAMYTYESSEQGDLTFQQGDVILVTKKDGDWWTGTVGDKAGVFPSNYVRLKDSEGSGTAGKTGSLGKKPEIAQVIASYTATGPEQLTLAPGQLILIRKKNPGGWWEGELQARGKKRQIGWFPANYVKLLSPGTSKITPTEPPKSTALAAVCQVIGMYDYTAQNDDELAFNKGQIINVLNKEDPDWWKGEVNGQVGLFPSNYVKLTTDMDPSQQWCSDLHLLDMLTPTERKRQGYIHELIVTEENYVNDLQLVTEIFQKPLMESELLTEKEVAMIFVNWKELIMCNIKLLKALRVRKKMSGEKMPVKMIGDILSAQLPHMQPYIRFCSRQLNGAALIQQKTDEAPDFKEFVKRLAMDPRCKGMPLSSFILKPMQRVTRYPLIIKNILENTPENHPDHSHLKHALEKAEELCSQVNEGVREKENSDRLEWIQAHVQCEGLSEQLVFNSVTNCLGPRKFLHSGKLYKAKSNKELYGFLFNDFLLLTQITKPLGSSGTDKVFSPKSNLQYKMYKTPIFLNEVLVKLPTDPSGDEPIFHISHIDRVYTLRAESINERTAWVQKIKAASELYIETEKKKREKAYLVRSQRATGIGRLMVNVVEGIELKPCRSHGKSNPYCEVTMGSQCHITKTIQDTLNPKWNSNCQFFIRDLEQEVLCITVFERDQFSPDDFLGRTEIRVADIKKDQGSKGPVTKCLLLHEVPTGEIVVRLDLQLFDEP

Target ORF information:

RefSeq Version	XM_017028428.1
Organism	Homo sapiens(human)
Definition	Homo sapiens intersectin 1 (ITSN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028428.1

ORF Insert Sequence:

ATGGCTCAGT TTCCAACACC TTTTGGTGGC AGCCTGGATA TCTGGGCCAT AACTGTAGAG 
GAAAGAGCGA AGCATGATCA GCAGTTCCAT AGTTTAAAGC CAATATCTGG ATTCATTACT 
GGTGATCAAG CTAGAAACTT TTTTTTTCAA TCTGGGTTAC CTCAACCTGT TTTAGCACAG 
ATATGGGCAC TAGCTGACAT GAATAATGAT GGAAGAATGG ATCAAGTGGA GTTTTCCATA 
GCTATGAAAC TTATCAAACT GAAGCTACAA GGATATCAGC TACCCTCTGC ACTTCCCCCT 
GTCATGAAAC AGCAACCAGT TGCTATTTCT AGCGCACCAG CATTTGGTAT GGGAGGTATC 
GCCAGCATGC CACCGCTTAC AGCTGTTGCT CCAGTGCCAA TGGGATCCAT TCCAGTTGTT 
GGAATGTCTC CAACCCTAGT ATCTTCTGTT CCCACAGCAG CTGTGCCCCC CCTGGCTAAC 
GGGGCTCCCC CTGTTATACA ACCTCTGCCT GCATTTGCTC ATCCTGCAGC CACATTGCCA 
AAGAGTTCTT CCTTTAGTAG ATCTGGTCCA GGGTCACAAC TAAACACTAA ATTACAAAAG 
GCACAGTCAT TTGATGTGGC CAGTGTCCCA CCAGTGGCAG AGTGGGCTGT TCCTCAGTCA 
TCAAGACTGA AATACAGGCA ATTATTCAAT AGTCATGACA AAACTATGAG TGGACACTTA 
ACAGGTCCCC AAGCAAGAAC TATTCTTATG CAGTCAAGTT TACCACAGGC TCAGCTGGCT 
TCAATATGGA ATCTTTCTGA CATTGATCAA GATGGAAAAC TTACAGCAGA GGAATTTATC 
CTGGCAATGC ACCTCATTGA TGTAGCTATG TCTGGCCAAC CACTGCCACC TGTCCTGCCT 
CCAGAATACA TTCCACCTTC TTTTAGAAGA GTTCGATCTG GCAGTGGTAT ATCTGTCATA 
AGCTCAACAT CTGTAGATCA GAGGCTACCA GAGGAACCAG TTTTAGAAGA TGAACAACAA 
CAATTAGAAA AGAAATTACC TGTAACGTTT GAAGATAAGA AGCGGGAGAA CTTTGAACGT 
GGCAACCTGG AACTGGAGAA ACGAAGGCAA GCTCTCCTGG AACAGCAGCG CAAGGAGCAG 
GAGCGCCTGG CCCAGCTGGA GCGGGCGGAG CAGGAGAGGA AGGAGCGTGA GCGCCAGGAG 
CAAGAGCGCA AAAGACAACT GGAACTGGAG AAGCAACTGG AAAAGCAGCG GGAGCTAGAA 
CGGCAGAGAG AGGAGGAGAG GAGGAAAGAA ATTGAGAGGC GAGAGGCTGC AAAACGGGAA 
CTTGAAAGGC AACGACAACT TGAGTGGGAA CGGAATCGAA GGCAAGAACT ACTAAATCAA 
AGAAACAAAG AACAAGAGGA CATAGTTGTA CTGAAAGCAA AGAAAAAGAC TTTGGAATTT 
GAATTAGAAG CTCTAAATGA TAAAAAGCAT CAACTAGAAG GGAAACTTCA AGATATCAGA 
TGTCGATTGA CCACCCAAAG GCAAGAAATT GAGAGCACAA ACAAATCTAG AGAGTTGAGA 
ATTGCCGAAA TCACCCATCT ACAGCAACAA TTACAGGAAT CTCAGCAAAT GCTTGGAAGA 
CTTATTCCAG AAAAACAGAT ACTCAATGAC CAATTAAAAC AAGTTCAGCA GAACAGTTTG 
CACAGAGATT CACTTGTTAC ACTTAAAAGA GCCTTAGAAG CAAAAGAACT AGCTCGGCAG 
CACCTACGAG ACCAACTGGA TGAAGTGGAG AAAGAAACTA GATCAAAACT ACAGGAGATT 
GATATTTTCA ATAATCAGCT GAAGGAACTA AGAGAAATAC ACAATAAGCA ACAACTCCAG 
AAGCAAAAGT CCATGGAGGC TGAACGACTG AAACAGAAAG AACAAGAACG AAAGATCATA 
GAATTAGAAA AACAAAAAGA AGAAGCCCAA AGACGAGCTC AGGAAAGGGA CAAGCAGTGG 
CTGGAGCATG TGCAGCAGGA GGACGAGCAT CAGAGACCAA GAAAACTCCA CGAAGAGGAA 
AAACTGAAAA GGGAGGAGAG TGTCAAAAAG AAGGATGGCG AGGAAAAAGG CAAACAGGAA 
GCACAAGACA AGCTGGGTCG GCTTTTCCAT CAACACCAAG AACCAGCTAA GCCAGCTGTC 
CAGGCACCCT GGTCCACTGC AGAAAAAGGT CCACTTACCA TTTCTGCACA GGAAAATGTA 
AAAGTGGTGT ATTACCGGGC ACTGTACCCC TTTGAATCCA GAAGCCATGA TGAAATCACT 
ATCCAGCCAG GAGACATAGT CATGGTTAAA GGGGAATGGG TGGATGAAAG CCAAACTGGA 
GAACCCGGCT GGCTTGGAGG AGAATTAAAA GGAAAGACAG GGTGGTTCCC TGCAAACTAT 
GCAGAGAAAA TCCCAGAAAA TGAGGTTCCC GCTCCAGTGA AACCAGTGAC TGATTCAACA 
TCTGCCCCTG CCCCCAAACT GGCCTTGCGT GAGACCCCCG CCCCTTTGGC AGTAACCTCT 
TCAGAGCCCT CCACGACCCC TAATAACTGG GCCGACTTCA GCTCCACGTG GCCCACCAGC 
ACGAATGAGA AACCAGAAAC GGATAACTGG GATGCATGGG CAGCCCAGCC CTCTCTCACC 
GTTCCAAGTG CCGGCCAGTT AAGGCAGAGG TCCGCCTTTA CTCCAGCCAC GGCCACTGGC 
TCCTCCCCGT CTCCTGTGCT AGGCCAGGGT GAAAAGGTGG AGGGGCTACA AGCTCAAGCC 
CTATATCCTT GGAGAGCCAA AAAAGACAAC CACTTAAATT TTAACAAAAA TGATGTCATC 
ACCGTCCTGG AACAGCAAGA CATGTGGTGG TTTGGAGAAG TTCAAGGTCA GAAGGGTTGG 
TTCCCCAAGT CTTACGTGAA ACTCATTTCA GGGCCCATAA GGAAGTCTAC AAGCATGGAT 
TCTGGTTCTT CAGAGAGTCC TGCTAGTCTA AAGCGAGTAG CCTCTCCAGC AGCCAAGCCG 
GTCGTTTCGG GAGAAGAATT TATTGCCATG TACACTTACG AGAGTTCTGA GCAAGGAGAT 
TTAACCTTTC AGCAAGGGGA TGTGATTTTG GTTACCAAGA AAGATGGTGA CTGGTGGACA 
GGAACAGTGG GCGACAAGGC CGGAGTCTTC CCTTCTAACT ATGTGAGGCT TAAAGATTCA 
GAGGGCTCTG GAACTGCTGG GAAAACAGGG AGTTTAGGAA AAAAACCTGA AATTGCCCAG 
GTTATTGCCT CATACACCGC CACCGGCCCC GAGCAGCTCA CTCTCGCCCC TGGTCAGCTG 
ATTTTGATCC GAAAAAAGAA CCCAGGTGGA TGGTGGGAAG GAGAGCTGCA AGCACGTGGG 
AAAAAGCGCC AGATAGGCTG GTTCCCAGCT AATTATGTAA AGCTTCTAAG CCCTGGGACG 
AGCAAAATCA CTCCAACAGA GCCACCTAAG TCAACAGCAT TAGCGGCAGT GTGCCAGGTG 
ATTGGGATGT ACGACTACAC CGCGCAGAAT GACGATGAGC TGGCCTTCAA CAAGGGCCAG 
ATCATCAACG TCCTCAACAA GGAGGACCCT GACTGGTGGA AAGGAGAAGT CAATGGACAA 
GTGGGGCTCT TCCCATCCAA TTATGTGAAG CTGACCACAG ACATGGACCC AAGCCAGCAA 
TGGTGTTCAG ACTTACATCT CTTGGATATG TTGACCCCAA CTGAAAGAAA GCGACAAGGA 
TACATCCACG AGCTCATTGT CACCGAGGAG AACTATGTGA ATGACCTGCA GCTGGTCACA 
GAGATTTTTC AAAAACCCCT GATGGAGTCT GAGCTGCTGA CAGAAAAAGA GGTTGCTATG 
ATTTTTGTGA ACTGGAAGGA GCTGATTATG TGTAATATCA AACTACTAAA AGCGCTGAGA 
GTCCGCAAGA AGATGTCCGG GGAGAAGATG CCTGTGAAGA TGATTGGAGA CATCCTGAGC 
GCACAGCTGC CGCACATGCA GCCCTACATC CGCTTCTGCA GCCGCCAGCT CAACGGGGCT 
GCCCTGATCC AGCAGAAGAC GGATGAGGCC CCAGACTTCA AGGAGTTCGT CAAAAGATTG 
GCAATGGATC CTCGGTGTAA AGGGATGCCA CTCTCTAGTT TTATACTGAA GCCTATGCAA 
CGGGTAACAA GATACCCACT GATCATTAAA AATATCCTGG AAAACACCCC TGAAAACCAC 
CCGGACCACA GCCACTTGAA GCACGCCCTG GAGAAGGCGG AAGAGCTCTG TTCCCAGGTG 
AACGAAGGGG TGCGGGAGAA GGAGAACTCT GACCGGCTGG AGTGGATCCA GGCCCACGTG 
CAGTGTGAAG GCCTGTCTGA GCAACTTGTG TTCAATTCAG TGACCAATTG CTTGGGGCCG 
CGCAAATTTC TGCACAGTGG GAAGCTCTAC AAGGCCAAGA GCAACAAGGA GCTGTATGGC 
TTCCTTTTCA ACGACTTCCT CCTGCTGACT CAGATCACGA AGCCTTTGGG GTCTTCTGGC 
ACCGACAAAG TCTTCAGCCC CAAATCAAAC CTGCAGTATA AAATGTATAA AACACCTATT 
TTCCTAAATG AGGTTCTAGT AAAATTACCC ACCGACCCTT CTGGAGACGA GCCCATCTTC 
CACATCTCCC ACATTGACCG CGTCTATACT CTCCGAGCAG AAAGCATAAA TGAAAGGACT 
GCCTGGGTGC AGAAAATCAA AGCTGCTTCT GAACTCTACA TAGAGACTGA GAAAAAGAAG 
CGCGAGAAAG CGTACCTGGT CCGTTCCCAA AGGGCAACAG GCATTGGAAG GTTGATGGTG 
AACGTGGTTG AAGGCATCGA GTTGAAACCC TGTCGGTCAC ATGGAAAGAG CAACCCGTAC 
TGTGAGGTGA CCATGGGTTC CCAGTGCCAC ATCACCAAGA CGATCCAGGA CACTCTGAAC 
CCCAAGTGGA ATTCCAACTG CCAGTTCTTC ATCCGAGACC TGGAGCAGGA AGTCCTCTGC 
ATCACTGTGT TCGAGAGGGA CCAGTTCTCA CCAGATGATT TTTTGGGTCG GACGGAGATC 
CGTGTGGCGG ACATCAAGAA AGACCAGGGC TCCAAAGGTC CAGTTACGAA GTGTCTTCTG 
CTGCACGAAG TCCCCACGGG AGAGATTGTG GTCCGCTTGG ACCTGCAGTT GTTTGATGAG 
CCGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Intersectin-s interaction with DENND2B facilitates recycling of epidermal growth factor receptor.
EMBO reports18(12)2119-2130(2017 Dec)
Ioannou MS,Kulasekaran G,Fotouhi M,Morein JJ,Han C,Tse S,Nossova N,Han T,Mannard E,McPherson PS

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ITSN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

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