MRPS25 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	MRPS25
Entrez Gene ID	64432
Full Name	mitochondrial ribosomal protein S25
Synonyms	MRP-S25,RPMS25
General protein information	Preferred Names mitochondrial ribosomal protein S25 Names 28S ribosomal protein S25, mitochondrial S25mt mitochondrial 28S ribosomal protein S25 mitochondrial small ribosomal subunit protein mS25
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p25.1
Summary	Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein. A pseudogene corresponding to this gene is found on chromosome 4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016].
Disorder MIM:	611987

mRNA and Protein(s)

mRNA	Protein	Name
NM_022497.4	NP_071942.1	28S ribosomal protein S25, mitochondrial
NM_022497.3	NP_071942.1	28S ribosomal protein S25, mitochondrial
NM_022497.5	NP_071942.1	28S ribosomal protein S25, mitochondrial

Pathways from BioSystems

Homologies

Homo sapiens (human)	MRPS25	NP_071942.1
	MRPS25	XP_001091560.2
Caenorhabditis elegans (roundworm)	mrps-25	NP_500032.1
Danio rerio (zebrafish)	mrps25	NP_001017590.1
Canis lupus familiaris (dog)	MRPS25	XP_533729.3
Rattus norvegicus (Norway rat)	Mrps25	NP_001020579.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012136	XP_320391.3
Xenopus tropicalis (tropical clawed frog)	LOC100494871	XP_002938273.1
Pan troglodytes (chimpanzee)	MRPS25	NP_001238852.1
Bos taurus (cattle)	MRPS25	NP_001069447.1
Mus musculus (house mouse)	Mrps25	NP_079854.2
Gallus gallus (chicken)	MRPS25	NP_001264769.1
Drosophila melanogaster (fruit fly)	mRpS25	NP_511153.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
Hendrickson SL, Lautenberger JA, Chinn LW, Malasky M, Sezgin E, Kingsley LA, Goedert JJ, Kirk GD, Gomperts ED, Buchbinder SP, Troyer JL, O'Brien SJ
PloS one5(9)e12862(2010 Sep)

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Girard A, Sachidanandam R, Hannon GJ, Carmell MA
Nature442(7099)199-202(2006 Jul)

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Zhang Z, Gerstein M
Genomics81(5)468-80(2003 May)

The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders.
Kenmochi N, Suzuki T, Uechi T, Magoori M, Kuniba M, Higa S, Watanabe K, Tanaka T
Genomics77(1-2)65-70(2001 Sep)

The small subunit of the mammalian mitochondrial ribosome. Identification of the full complement of ribosomal proteins present.
Cavdar Koc E, Burkhart W, Blackburn K, Moseley A, Spremulli LL
The Journal of biological chemistry276(22)19363-74(2001 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

The following MRPS25 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MRPS25 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu13072	NM_022497.5 Latest version!	Homo sapiens mitochondrial ribosomal protein S25 (MRPS25), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu13072	NM_022497.3	Homo sapiens mitochondrial ribosomal protein S25 (MRPS25), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu13072	NM_022497.4	Homo sapiens mitochondrial ribosomal protein S25 (MRPS25), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MRPS25 ( NM_022497.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_071942.1 Homo sapiens mitochondrial ribosomal protein S25 (MRPS25), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13072
Clone ID Related Accession (Same CDS sequence)	NM_022497.4 , NM_022497.3 , NM_022497.5
Accession Version	NM_022497.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	28S ribosomal protein S25, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA729310.1, BC003590.2, AK024433.1 and AI288348.1. On Feb 25, 2016 this sequence version replaced NM_022497.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the supported protein. ##Evidence-Data-START## Transcript exon combination :: DA729310.1, SRR1803614.269713.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119, SAMEA2156266 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCCATGA AGGGCCGCTT CCCCATCCGC CGCACCCTGC AATATCTGAG CCAGGGGAAC 
GTGGTGTTCA AGGACTCCGT GAAGGTCATG ACAGTGAATT ACAACACGCA TGGGGAGCTG 
GGCGAGGGCG CCAGGAAGTT TGTGTTTTTC AACATACCTC AGATTCAATA CAAAAACCCT 
TGGGTGCAGA TCATGATGTT TAAGAACATG ACGCCGTCAC CCTTCCTGCG ATTCTACTTA 
GATTCTGGGG AGCAGGTCCT GGTGGATGTG GAGACCAAGA GCAATAAGGA GATCATGGAG 
CACATCAGAA AAATCTTGGG GAAGAATGAG GAAACCCTCA GGGAAGAGGA GGAGGAGAAA 
AAGCAGCTTT CTCACCCAGC CAACTTCGGC CCTCGAAAGT ACTGCCTGCG GGAGTGCATC 
TGTGAAGTGG AAGGGCAGGT GCCCTGCCCC AGCCTGGTGC CATTACCCAA GGAGATGAGG 
GGGAAGTACA AAGCCGCTCT GAAAGCCGAT GCCCAGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071942.1
CDS	144..665
Translation	MPMKGRFPIRRTLQYLSQGNVVFKDSVKVMTVNYNTHGELGEGARKFVFFNIPQIQYKNPWVQIMMFKNMTPSPFLRFYLDSGEQVLVDVETKSNKEIMEHIRKILGKNEETLREEEEEKKQLSHPANFGPRKYCLRECICEVEGQVPCPSLVPLPKEMRGKYKAALKADAQD

Target ORF information:

RefSeq Version	NM_022497.4
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S25 (MRPS25), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022497.4

ORF Insert Sequence:

ATGCCCATGA AGGGCCGCTT CCCCATCCGC CGCACCCTGC AATATCTGAG CCAGGGGAAC 
GTGGTGTTCA AGGACTCCGT GAAGGTCATG ACAGTGAATT ACAACACGCA TGGGGAGCTG 
GGCGAGGGCG CCAGGAAGTT TGTGTTTTTC AACATACCTC AGATTCAATA CAAAAACCCT 
TGGGTGCAGA TCATGATGTT TAAGAACATG ACGCCGTCAC CCTTCCTGCG ATTCTACTTA 
GATTCTGGGG AGCAGGTCCT GGTGGATGTG GAGACCAAGA GCAATAAGGA GATCATGGAG 
CACATCAGAA AAATCTTGGG GAAGAATGAG GAAACCCTCA GGGAAGAGGA GGAGGAGAAA 
AAGCAGCTTT CTCACCCAGC CAACTTCGGC CCTCGAAAGT ACTGCCTGCG GGAGTGCATC 
TGTGAAGTGG AAGGGCAGGT GCCCTGCCCC AGCCTGGTGC CATTACCCAA GGAGATGAGG 
GGGAAGTACA AAGCCGCTCT GAAAGCCGAT GCCCAGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPS25 ( NM_022497.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_071942.1 Homo sapiens mitochondrial ribosomal protein S25 (MRPS25), mRNA.

Price & Availability↑

CloneID	OHu13072
Clone ID Related Accession (Same CDS sequence)	NM_022497.4 , NM_022497.3 , NM_022497.5
Accession Version	NM_022497.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-14
Organism	Homo sapiens(Human)
Product	28S ribosomal protein S25, mitochondrial
Comment

ATGCCCATGA AGGGCCGCTT CCCCATCCGC CGCACCCTGC AATATCTGAG CCAGGGGAAC 
GTGGTGTTCA AGGACTCCGT GAAGGTCATG ACAGTGAATT ACAACACGCA TGGGGAGCTG 
GGCGAGGGCG CCAGGAAGTT TGTGTTTTTC AACATACCTC AGATTCAATA CAAAAACCCT 
TGGGTGCAGA TCATGATGTT TAAGAACATG ACGCCGTCAC CCTTCCTGCG ATTCTACTTA 
GATTCTGGGG AGCAGGTCCT GGTGGATGTG GAGACCAAGA GCAATAAGGA GATCATGGAG 
CACATCAGAA AAATCTTGGG GAAGAATGAG GAAACCCTCA GGGAAGAGGA GGAGGAGAAA 
AAGCAGCTTT CTCACCCAGC CAACTTCGGC CCTCGAAAGT ACTGCCTGCG GGAGTGCATC 
TGTGAAGTGG AAGGGCAGGT GCCCTGCCCC AGCCTGGTGC CATTACCCAA GGAGATGAGG 
GGGAAGTACA AAGCCGCTCT GAAAGCCGAT GCCCAGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071942.1
Translation	MPMKGRFPIRRTLQYLSQGNVVFKDSVKVMTVNYNTHGELGEGARKFVFFNIPQIQYKNPWVQIMMFKNMTPSPFLRFYLDSGEQVLVDVETKSNKEIMEHIRKILGKNEETLREEEEEKKQLSHPANFGPRKYCLRECICEVEGQVPCPSLVPLPKEMRGKYKAALKADAQD

Target ORF information:

RefSeq Version	NM_022497.3
Organism	Homo sapiens(Human)
Definition	Homo sapiens mitochondrial ribosomal protein S25 (MRPS25), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022497.3

ORF Insert Sequence:

ATGCCCATGA AGGGCCGCTT CCCCATCCGC CGCACCCTGC AATATCTGAG CCAGGGGAAC 
GTGGTGTTCA AGGACTCCGT GAAGGTCATG ACAGTGAATT ACAACACGCA TGGGGAGCTG 
GGCGAGGGCG CCAGGAAGTT TGTGTTTTTC AACATACCTC AGATTCAATA CAAAAACCCT 
TGGGTGCAGA TCATGATGTT TAAGAACATG ACGCCGTCAC CCTTCCTGCG ATTCTACTTA 
GATTCTGGGG AGCAGGTCCT GGTGGATGTG GAGACCAAGA GCAATAAGGA GATCATGGAG 
CACATCAGAA AAATCTTGGG GAAGAATGAG GAAACCCTCA GGGAAGAGGA GGAGGAGAAA 
AAGCAGCTTT CTCACCCAGC CAACTTCGGC CCTCGAAAGT ACTGCCTGCG GGAGTGCATC 
TGTGAAGTGG AAGGGCAGGT GCCCTGCCCC AGCCTGGTGC CATTACCCAA GGAGATGAGG 
GGGAAGTACA AAGCCGCTCT GAAAGCCGAT GCCCAGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MRPS25 ( NM_022497.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_071942.1 Homo sapiens mitochondrial ribosomal protein S25 (MRPS25), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13072
Clone ID Related Accession (Same CDS sequence)	NM_022497.4 , NM_022497.3 , NM_022497.5
Accession Version	NM_022497.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	28S ribosomal protein S25, mitochondrial
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA729310.1, BC003590.2, AK024433.1 and AI288348.1. On Nov 23, 2018 this sequence version replaced NM_022497.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the supported protein. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.204630.1, SRR1803614.269713.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2151119, SAMEA2156266 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000253686.7/ ENSP00000253686.2 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCATGA AGGGCCGCTT CCCCATCCGC CGCACCCTGC AATATCTGAG CCAGGGGAAC 
GTGGTGTTCA AGGACTCCGT GAAGGTCATG ACAGTGAATT ACAACACGCA TGGGGAGCTG 
GGCGAGGGCG CCAGGAAGTT TGTGTTTTTC AACATACCTC AGATTCAATA CAAAAACCCT 
TGGGTGCAGA TCATGATGTT TAAGAACATG ACGCCGTCAC CCTTCCTGCG ATTCTACTTA 
GATTCTGGGG AGCAGGTCCT GGTGGATGTG GAGACCAAGA GCAATAAGGA GATCATGGAG 
CACATCAGAA AAATCTTGGG GAAGAATGAG GAAACCCTCA GGGAAGAGGA GGAGGAGAAA 
AAGCAGCTTT CTCACCCAGC CAACTTCGGC CCTCGAAAGT ACTGCCTGCG GGAGTGCATC 
TGTGAAGTGG AAGGGCAGGT GCCCTGCCCC AGCCTGGTGC CATTACCCAA GGAGATGAGG 
GGGAAGTACA AAGCCGCTCT GAAAGCCGAT GCCCAGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071942.1
CDS	122..643
Translation	MPMKGRFPIRRTLQYLSQGNVVFKDSVKVMTVNYNTHGELGEGARKFVFFNIPQIQYKNPWVQIMMFKNMTPSPFLRFYLDSGEQVLVDVETKSNKEIMEHIRKILGKNEETLREEEEEKKQLSHPANFGPRKYCLRECICEVEGQVPCPSLVPLPKEMRGKYKAALKADAQD

Target ORF information:

RefSeq Version	NM_022497.5
Organism	Homo sapiens(human)
Definition	Homo sapiens mitochondrial ribosomal protein S25 (MRPS25), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022497.5

ORF Insert Sequence:

ATGCCCATGA AGGGCCGCTT CCCCATCCGC CGCACCCTGC AATATCTGAG CCAGGGGAAC 
GTGGTGTTCA AGGACTCCGT GAAGGTCATG ACAGTGAATT ACAACACGCA TGGGGAGCTG 
GGCGAGGGCG CCAGGAAGTT TGTGTTTTTC AACATACCTC AGATTCAATA CAAAAACCCT 
TGGGTGCAGA TCATGATGTT TAAGAACATG ACGCCGTCAC CCTTCCTGCG ATTCTACTTA 
GATTCTGGGG AGCAGGTCCT GGTGGATGTG GAGACCAAGA GCAATAAGGA GATCATGGAG 
CACATCAGAA AAATCTTGGG GAAGAATGAG GAAACCCTCA GGGAAGAGGA GGAGGAGAAA 
AAGCAGCTTT CTCACCCAGC CAACTTCGGC CCTCGAAAGT ACTGCCTGCG GGAGTGCATC 
TGTGAAGTGG AAGGGCAGGT GCCCTGCCCC AGCCTGGTGC CATTACCCAA GGAGATGAGG 
GGGAAGTACA AAGCCGCTCT GAAAGCCGAT GCCCAGGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.
PloS one5(9)e12862(2010 Sep)
Hendrickson SL,Lautenberger JA,Chinn LW,Malasky M,Sezgin E,Kingsley LA,Goedert JJ,Kirk GD,Gomperts ED,Buchbinder SP,Troyer JL,O'Brien SJ

A germline-specific class of small RNAs binds mammalian Piwi proteins.
Nature442(7099)199-202(2006 Jul)
Girard A,Sachidanandam R,Hannon GJ,Carmell MA

Identification and characterization of over 100 mitochondrial ribosomal protein pseudogenes in the human genome.
Genomics81(5)468-80(2003 May)
Zhang Z,Gerstein M

The human mitochondrial ribosomal protein genes: mapping of 54 genes to the chromosomes and implications for human disorders.
Genomics77(1-2)65-70(2001 Sep)
Kenmochi N,Suzuki T,Uechi T,Magoori M,Kuniba M,Higa S,Watanabe K,Tanaka T

The small subunit of the mammalian mitochondrial ribosome. Identification of the full complement of ribosomal proteins present.
The Journal of biological chemistry276(22)19363-74(2001 Jun)
Cavdar Koc E,Burkhart W,Blackburn K,Moseley A,Spremulli LL

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

MRPS25 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報