SIL1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SIL1
Entrez Gene ID	64374
Full Name	SIL1 nucleotide exchange factor
Synonyms	BAP,MSS,ULG5
General protein information	Preferred Names SIL1 nucleotide exchange factor Names nucleotide exchange factor SIL1 BiP-associated protein SIL1 homolog, endoplasmic reticulum chaperone SIL1-like protein endoplasmic reticulum chaperone
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 5 Map Location: 5q31.2
Summary	This gene encodes a resident endoplasmic reticulum (ER), N-linked glycoprotein with an N-terminal ER targeting sequence, 2 putative N-glycosylation sites, and a C-terminal ER retention signal. This protein functions as a nucleotide exchange factor for another unfolded protein response protein. Mutations in this gene have been associated with Marinesco-Sjogren syndrome. Alternate transcriptional splice variants have been characterized. [provided by RefSeq, Jul 2008].
Disorder MIM:	608005
Disorder Html:	Marinesco-Sjogren syndrome, 248800 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001037633.1	NP_001032722.1	nucleotide exchange factor SIL1 precursor
XM_011543570.2	XP_011541872.1	nucleotide exchange factor SIL1 isoform X1
NM_022464.4	NP_071909.1	nucleotide exchange factor SIL1 precursor
NM_022464.5	NP_071909.1	nucleotide exchange factor SIL1 precursor
NM_001037633.2	NP_001032722.1	nucleotide exchange factor SIL1 precursor
XM_024446164.1	XP_024301932.1	nucleotide exchange factor SIL1 isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	SIL1	NP_001069276.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003488	XP_309679.5
Xenopus tropicalis (tropical clawed frog)	sil1	NP_001096338.1
Rattus norvegicus (Norway rat)	Sil1	NP_955408.1
Danio rerio (zebrafish)	LOC100334837	XP_002664491.2
Danio rerio (zebrafish)	LOC568308	XP_696722.3
	SIL1	XP_001114098.1
Mus musculus (house mouse)	Sil1	NP_109674.2
Gallus gallus (chicken)	SIL1	XP_004944829.1
Homo sapiens (human)	SIL1	NP_071909.1
Pan troglodytes (chimpanzee)	SIL1	XP_001157060.2
Drosophila melanogaster (fruit fly)	CG10420	NP_651356.1
Canis lupus familiaris (dog)	SIL1	XP_005626665.1

Gene Ontology
Bibliography

Related articles in PubMed

Bap (Sil1) regulates the molecular chaperone BiP by coupling release of nucleotide and substrate.
Rosam M, Krader D, Nickels C, Hochmair J, Back KC, Agam G, Barth A, Zeymer C, Hendrix J, Schneider M, Antes I, Reinstein J, Lamb DC, Buchner J
Nature structural & molecular biology25(1)90-100(2018 Jan)

Novel SIL1 nonstop mutation in a Chinese consanguineous family with Marinesco-Sj?gren syndrome and Dandy-Walker syndrome.
Gai N, Jiang C, Zou YY, Zheng Y, Liang DS, Wu LQ
Clinica chimica acta; international journal of clinical chemistry4581-4(2016 Jul)

Marinesco-Sj?gren Syndrome in an Emirati Child with a Novel Mutation in SIL1 Affecting the 5' Untranslated Region.
Nair P, Hamzeh AR, Mohamed M, Tawfiq N, Al-Ali MT, Bastaki F
Medical principles and practice : international journal of the Kuwait University, Health Science Centre25(6)580-582(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

this case study is the first report on Chinese Marinesco-Sjogren syndrome (MSS) patients, MSS complicated by Dandy-Walker syndrome (DWS), and a nonstop mutation in SIL1; our findings imply the pathogenetic association between DWS and MSS
Title: Novel SIL1 nonstop mutation in a Chinese consanguineous family with Marinesco-Sj?gren syndrome and Dandy-Walker syndrome.

In a child with Marinesco-Sjogren syndrome it was found that a mutation in SIL1 affected the 5' UTR, translation initiation site and the endoplasmic reticulum-targeting signal sequence.
Title: Marinesco-Sj?gren Syndrome in an Emirati Child with a Novel Mutation in SIL1 Affecting the 5' Untranslated Region.

Two NEFs, Grp170 and Sil1, trigger toxin release from BiP to enable successful retrotranslocation and clarify the fate of the toxin after it disengages from BiP.
Title: The nucleotide exchange factors Grp170 and Sil1 induce cholera toxin release from BiP to enable retrotranslocation.

This study demonistrated that SIL1 mutation in patient with ataxia telangiectasia
Title: Whole-exome sequence analysis of ataxia telangiectasia-like phenotype.

The mutations prevent SIL1 from interacting with and regulating HSPA5, leading to abnormal neuronal morphology and migration.
Title: SIL1, a causative cochaperone gene of Marinesco-S?jgren syndrome, plays an essential role in establishing the architecture of the developing cerebral cortex.

The following SIL1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SIL1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu23128	NM_001037633.2 Latest version!	Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu23128	NM_022464.5 Latest version!	Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu23128	XM_024446164.1 Latest version!	Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu39670	XM_011543570.2 Latest version!	Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu23128	NM_022464.4	Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
OHu23128	NM_001037633.1	Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SIL1 ( NM_001037633.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001032722.1 Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23128
Clone ID Related Accession (Same CDS sequence)	NM_001037633.1 , NM_022464.4 , NM_022464.5 , NM_001037633.2 , XM_024446164.1
Accession Version	NM_001037633.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	nucleotide exchange factor SIL1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB125000.1, AC008614.6, AC011378.7, AC011405.6, CD369479.1 and CA415266.1. This sequence is a reference standard in the RefSeqGene project. On May 31, 2019 this sequence version replaced NM_001037633.1. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: SRR1163658.490814.1, SRR1803614.278267.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCCC AGAGCCTGCC TTCATCTAGG ATGGCTCCTC TGGGCATGCT GCTTGGGCTG 
CTGATGGCCG CCTGCTTCAC CTTCTGCCTC AGTCATCAGA ACCTGAAGGA GTTTGCCCTG 
ACCAACCCAG AGAAGAGCAG CACCAAAGAA ACAGAGAGAA AAGAAACCAA AGCCGAGGAG 
GAGCTGGATG CCGAAGTCCT GGAGGTGTTC CACCCGACGC ATGAGTGGCA GGCCCTTCAG 
CCAGGGCAGG CTGTCCCTGC AGGATCCCAC GTACGGCTGA ATCTTCAGAC TGGGGAAAGA 
GAGGCAAAAC TCCAATATGA GGACAAGTTC CGAAATAATT TGAAAGGCAA AAGGCTGGAT 
ATCAACACCA ACACCTACAC ATCTCAGGAT CTCAAGAGTG CACTGGCAAA ATTCAAGGAG 
GGGGCAGAGA TGGAGAGTTC AAAGGAAGAC AAGGCAAGGC AGGCTGAGGT AAAGCGGCTC 
TTCCGCCCCA TTGAGGAACT GAAGAAAGAC TTTGATGAGC TGAATGTTGT CATTGAGACT 
GACATGCAGA TCATGGTACG GCTGATCAAC AAGTTCAATA GTTCCAGCTC CAGTTTGGAA 
GAGAAGATTG CTGCGCTCTT TGATCTTGAA TATTATGTCC ATCAGATGGA CAATGCGCAG 
GACCTGCTTT CCTTTGGTGG TCTTCAAGTG GTGATCAATG GGCTGAACAG CACAGAGCCC 
CTCGTGAAGG AGTATGCTGC GTTTGTGCTG GGCGCTGCCT TTTCCAGCAA CCCCAAGGTC 
CAGGTGGAGG CCATCGAAGG GGGAGCCCTG CAGAAGCTGC TGGTCATCCT GGCCACGGAG 
CAGCCGCTCA CTGCAAAGAA GAAGGTCCTG TTTGCACTGT GCTCCCTGCT GCGCCACTTC 
CCCTATGCCC AGCGGCAGTT CCTGAAGCTC GGGGGGCTGC AGGTCCTGAG GACCCTGGTG 
CAGGAGAAGG GCACGGAGGT GCTCGCCGTG CGCGTGGTCA CACTGCTCTA CGACCTGGTC 
ACGGAGAAGA TGTTCGCCGA GGAGGAGGCT GAGCTGACCC AGGAGATGTC CCCAGAGAAG 
CTGCAGCAGT ATCGCCAGGT ACACCTCCTG CCAGGCCTGT GGGAACAGGG CTGGTGCGAG 
ATCACGGCCC ACCTCCTGGC GCTGCCCGAG CATGATGCCC GTGAGAAGGT GCTGCAGACA 
CTGGGCGTCC TCCTGACCAC CTGCCGGGAC CGCTACCGTC AGGACCCCCA GCTCGGCAGG 
ACACTGGCCA GCCTGCAGGC TGAGTACCAG GTGCTGGCCA GCCTGGAGCT GCAGGATGGT 
GAGGACGAGG GCTACTTCCA GGAGCTGCTG GGCTCTGTCA ACAGCTTGCT GAAGGAGCTG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032722.1
CDS	199..1584
Translation	MAPQSLPSSRMAPLGMLLGLLMAACFTFCLSHQNLKEFALTNPEKSSTKETERKETKAEEELDAEVLEVFHPTHEWQALQPGQAVPAGSHVRLNLQTGEREAKLQYEDKFRNNLKGKRLDINTNTYTSQDLKSALAKFKEGAEMESSKEDKARQAEVKRLFRPIEELKKDFDELNVVIETDMQIMVRLINKFNSSSSSLEEKIAALFDLEYYVHQMDNAQDLLSFGGLQVVINGLNSTEPLVKEYAAFVLGAAFSSNPKVQVEAIEGGALQKLLVILATEQPLTAKKKVLFALCSLLRHFPYAQRQFLKLGGLQVLRTLVQEKGTEVLAVRVVTLLYDLVTEKMFAEEEAELTQEMSPEKLQQYRQVHLLPGLWEQGWCEITAHLLALPEHDAREKVLQTLGVLLTTCRDRYRQDPQLGRTLASLQAEYQVLASLELQDGEDEGYFQELLGSVNSLLKELR

Target ORF information:

RefSeq Version	NM_001037633.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037633.2

ORF Insert Sequence:

ATGGCTCCCC AGAGCCTGCC TTCATCTAGG ATGGCTCCTC TGGGCATGCT GCTTGGGCTG 
CTGATGGCCG CCTGCTTCAC CTTCTGCCTC AGTCATCAGA ACCTGAAGGA GTTTGCCCTG 
ACCAACCCAG AGAAGAGCAG CACCAAAGAA ACAGAGAGAA AAGAAACCAA AGCCGAGGAG 
GAGCTGGATG CCGAAGTCCT GGAGGTGTTC CACCCGACGC ATGAGTGGCA GGCCCTTCAG 
CCAGGGCAGG CTGTCCCTGC AGGATCCCAC GTACGGCTGA ATCTTCAGAC TGGGGAAAGA 
GAGGCAAAAC TCCAATATGA GGACAAGTTC CGAAATAATT TGAAAGGCAA AAGGCTGGAT 
ATCAACACCA ACACCTACAC ATCTCAGGAT CTCAAGAGTG CACTGGCAAA ATTCAAGGAG 
GGGGCAGAGA TGGAGAGTTC AAAGGAAGAC AAGGCAAGGC AGGCTGAGGT AAAGCGGCTC 
TTCCGCCCCA TTGAGGAACT GAAGAAAGAC TTTGATGAGC TGAATGTTGT CATTGAGACT 
GACATGCAGA TCATGGTACG GCTGATCAAC AAGTTCAATA GTTCCAGCTC CAGTTTGGAA 
GAGAAGATTG CTGCGCTCTT TGATCTTGAA TATTATGTCC ATCAGATGGA CAATGCGCAG 
GACCTGCTTT CCTTTGGTGG TCTTCAAGTG GTGATCAATG GGCTGAACAG CACAGAGCCC 
CTCGTGAAGG AGTATGCTGC GTTTGTGCTG GGCGCTGCCT TTTCCAGCAA CCCCAAGGTC 
CAGGTGGAGG CCATCGAAGG GGGAGCCCTG CAGAAGCTGC TGGTCATCCT GGCCACGGAG 
CAGCCGCTCA CTGCAAAGAA GAAGGTCCTG TTTGCACTGT GCTCCCTGCT GCGCCACTTC 
CCCTATGCCC AGCGGCAGTT CCTGAAGCTC GGGGGGCTGC AGGTCCTGAG GACCCTGGTG 
CAGGAGAAGG GCACGGAGGT GCTCGCCGTG CGCGTGGTCA CACTGCTCTA CGACCTGGTC 
ACGGAGAAGA TGTTCGCCGA GGAGGAGGCT GAGCTGACCC AGGAGATGTC CCCAGAGAAG 
CTGCAGCAGT ATCGCCAGGT ACACCTCCTG CCAGGCCTGT GGGAACAGGG CTGGTGCGAG 
ATCACGGCCC ACCTCCTGGC GCTGCCCGAG CATGATGCCC GTGAGAAGGT GCTGCAGACA 
CTGGGCGTCC TCCTGACCAC CTGCCGGGAC CGCTACCGTC AGGACCCCCA GCTCGGCAGG 
ACACTGGCCA GCCTGCAGGC TGAGTACCAG GTGCTGGCCA GCCTGGAGCT GCAGGATGGT 
GAGGACGAGG GCTACTTCCA GGAGCTGCTG GGCTCTGTCA ACAGCTTGCT GAAGGAGCTG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIL1 ( NM_001037633.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001032722.1 Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu23128
Clone ID Related Accession (Same CDS sequence)	NM_001037633.1 , NM_022464.4 , NM_022464.5 , NM_001037633.2 , XM_024446164.1
Accession Version	NM_001037633.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-16
Organism	Homo sapiens(human)
Product	nucleotide exchange factor SIL1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB125000.1, AC008614.6, AC011378.7, AC011405.6, CD369479.1 and CA415266.1. This sequence is a reference standard in the RefSeqGene project. Transcript Variant: This variant (1) represents the longer transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: AF547994.1, SRR1163655.303463.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCCC AGAGCCTGCC TTCATCTAGG ATGGCTCCTC TGGGCATGCT GCTTGGGCTG 
CTGATGGCCG CCTGCTTCAC CTTCTGCCTC AGTCATCAGA ACCTGAAGGA GTTTGCCCTG 
ACCAACCCAG AGAAGAGCAG CACCAAAGAA ACAGAGAGAA AAGAAACCAA AGCCGAGGAG 
GAGCTGGATG CCGAAGTCCT GGAGGTGTTC CACCCGACGC ATGAGTGGCA GGCCCTTCAG 
CCAGGGCAGG CTGTCCCTGC AGGATCCCAC GTACGGCTGA ATCTTCAGAC TGGGGAAAGA 
GAGGCAAAAC TCCAATATGA GGACAAGTTC CGAAATAATT TGAAAGGCAA AAGGCTGGAT 
ATCAACACCA ACACCTACAC ATCTCAGGAT CTCAAGAGTG CACTGGCAAA ATTCAAGGAG 
GGGGCAGAGA TGGAGAGTTC AAAGGAAGAC AAGGCAAGGC AGGCTGAGGT AAAGCGGCTC 
TTCCGCCCCA TTGAGGAACT GAAGAAAGAC TTTGATGAGC TGAATGTTGT CATTGAGACT 
GACATGCAGA TCATGGTACG GCTGATCAAC AAGTTCAATA GTTCCAGCTC CAGTTTGGAA 
GAGAAGATTG CTGCGCTCTT TGATCTTGAA TATTATGTCC ATCAGATGGA CAATGCGCAG 
GACCTGCTTT CCTTTGGTGG TCTTCAAGTG GTGATCAATG GGCTGAACAG CACAGAGCCC 
CTCGTGAAGG AGTATGCTGC GTTTGTGCTG GGCGCTGCCT TTTCCAGCAA CCCCAAGGTC 
CAGGTGGAGG CCATCGAAGG GGGAGCCCTG CAGAAGCTGC TGGTCATCCT GGCCACGGAG 
CAGCCGCTCA CTGCAAAGAA GAAGGTCCTG TTTGCACTGT GCTCCCTGCT GCGCCACTTC 
CCCTATGCCC AGCGGCAGTT CCTGAAGCTC GGGGGGCTGC AGGTCCTGAG GACCCTGGTG 
CAGGAGAAGG GCACGGAGGT GCTCGCCGTG CGCGTGGTCA CACTGCTCTA CGACCTGGTC 
ACGGAGAAGA TGTTCGCCGA GGAGGAGGCT GAGCTGACCC AGGAGATGTC CCCAGAGAAG 
CTGCAGCAGT ATCGCCAGGT ACACCTCCTG CCAGGCCTGT GGGAACAGGG CTGGTGCGAG 
ATCACGGCCC ACCTCCTGGC GCTGCCCGAG CATGATGCCC GTGAGAAGGT GCTGCAGACA 
CTGGGCGTCC TCCTGACCAC CTGCCGGGAC CGCTACCGTC AGGACCCCCA GCTCGGCAGG 
ACACTGGCCA GCCTGCAGGC TGAGTACCAG GTGCTGGCCA GCCTGGAGCT GCAGGATGGT 
GAGGACGAGG GCTACTTCCA GGAGCTGCTG GGCTCTGTCA ACAGCTTGCT GAAGGAGCTG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001032722.1
CDS	207..1592
Translation	MAPQSLPSSRMAPLGMLLGLLMAACFTFCLSHQNLKEFALTNPEKSSTKETERKETKAEEELDAEVLEVFHPTHEWQALQPGQAVPAGSHVRLNLQTGEREAKLQYEDKFRNNLKGKRLDINTNTYTSQDLKSALAKFKEGAEMESSKEDKARQAEVKRLFRPIEELKKDFDELNVVIETDMQIMVRLINKFNSSSSSLEEKIAALFDLEYYVHQMDNAQDLLSFGGLQVVINGLNSTEPLVKEYAAFVLGAAFSSNPKVQVEAIEGGALQKLLVILATEQPLTAKKKVLFALCSLLRHFPYAQRQFLKLGGLQVLRTLVQEKGTEVLAVRVVTLLYDLVTEKMFAEEEAELTQEMSPEKLQQYRQVHLLPGLWEQGWCEITAHLLALPEHDAREKVLQTLGVLLTTCRDRYRQDPQLGRTLASLQAEYQVLASLELQDGEDEGYFQELLGSVNSLLKELR

Target ORF information:

RefSeq Version	NM_001037633.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001037633.1

ORF Insert Sequence:

ATGGCTCCCC AGAGCCTGCC TTCATCTAGG ATGGCTCCTC TGGGCATGCT GCTTGGGCTG 
CTGATGGCCG CCTGCTTCAC CTTCTGCCTC AGTCATCAGA ACCTGAAGGA GTTTGCCCTG 
ACCAACCCAG AGAAGAGCAG CACCAAAGAA ACAGAGAGAA AAGAAACCAA AGCCGAGGAG 
GAGCTGGATG CCGAAGTCCT GGAGGTGTTC CACCCGACGC ATGAGTGGCA GGCCCTTCAG 
CCAGGGCAGG CTGTCCCTGC AGGATCCCAC GTACGGCTGA ATCTTCAGAC TGGGGAAAGA 
GAGGCAAAAC TCCAATATGA GGACAAGTTC CGAAATAATT TGAAAGGCAA AAGGCTGGAT 
ATCAACACCA ACACCTACAC ATCTCAGGAT CTCAAGAGTG CACTGGCAAA ATTCAAGGAG 
GGGGCAGAGA TGGAGAGTTC AAAGGAAGAC AAGGCAAGGC AGGCTGAGGT AAAGCGGCTC 
TTCCGCCCCA TTGAGGAACT GAAGAAAGAC TTTGATGAGC TGAATGTTGT CATTGAGACT 
GACATGCAGA TCATGGTACG GCTGATCAAC AAGTTCAATA GTTCCAGCTC CAGTTTGGAA 
GAGAAGATTG CTGCGCTCTT TGATCTTGAA TATTATGTCC ATCAGATGGA CAATGCGCAG 
GACCTGCTTT CCTTTGGTGG TCTTCAAGTG GTGATCAATG GGCTGAACAG CACAGAGCCC 
CTCGTGAAGG AGTATGCTGC GTTTGTGCTG GGCGCTGCCT TTTCCAGCAA CCCCAAGGTC 
CAGGTGGAGG CCATCGAAGG GGGAGCCCTG CAGAAGCTGC TGGTCATCCT GGCCACGGAG 
CAGCCGCTCA CTGCAAAGAA GAAGGTCCTG TTTGCACTGT GCTCCCTGCT GCGCCACTTC 
CCCTATGCCC AGCGGCAGTT CCTGAAGCTC GGGGGGCTGC AGGTCCTGAG GACCCTGGTG 
CAGGAGAAGG GCACGGAGGT GCTCGCCGTG CGCGTGGTCA CACTGCTCTA CGACCTGGTC 
ACGGAGAAGA TGTTCGCCGA GGAGGAGGCT GAGCTGACCC AGGAGATGTC CCCAGAGAAG 
CTGCAGCAGT ATCGCCAGGT ACACCTCCTG CCAGGCCTGT GGGAACAGGG CTGGTGCGAG 
ATCACGGCCC ACCTCCTGGC GCTGCCCGAG CATGATGCCC GTGAGAAGGT GCTGCAGACA 
CTGGGCGTCC TCCTGACCAC CTGCCGGGAC CGCTACCGTC AGGACCCCCA GCTCGGCAGG 
ACACTGGCCA GCCTGCAGGC TGAGTACCAG GTGCTGGCCA GCCTGGAGCT GCAGGATGGT 
GAGGACGAGG GCTACTTCCA GGAGCTGCTG GGCTCTGTCA ACAGCTTGCT GAAGGAGCTG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIL1 ( NM_022464.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_071909.1 Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu23128
Clone ID Related Accession (Same CDS sequence)	NM_001037633.1 , NM_022464.4 , NM_022464.5 , NM_001037633.2 , XM_024446164.1
Accession Version	NM_022464.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	nucleotide exchange factor SIL1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI561751.1, AK074624.1, BC011568.1, CD369479.1 and CA415266.1. On Dec 14, 2005 this sequence version replaced NM_022464.3. Transcript Variant: This variant (2) lacks an exon in the 5'UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.658092.1, AF547994.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTCCCC AGAGCCTGCC TTCATCTAGG ATGGCTCCTC TGGGCATGCT GCTTGGGCTG 
CTGATGGCCG CCTGCTTCAC CTTCTGCCTC AGTCATCAGA ACCTGAAGGA GTTTGCCCTG 
ACCAACCCAG AGAAGAGCAG CACCAAAGAA ACAGAGAGAA AAGAAACCAA AGCCGAGGAG 
GAGCTGGATG CCGAAGTCCT GGAGGTGTTC CACCCGACGC ATGAGTGGCA GGCCCTTCAG 
CCAGGGCAGG CTGTCCCTGC AGGATCCCAC GTACGGCTGA ATCTTCAGAC TGGGGAAAGA 
GAGGCAAAAC TCCAATATGA GGACAAGTTC CGAAATAATT TGAAAGGCAA AAGGCTGGAT 
ATCAACACCA ACACCTACAC ATCTCAGGAT CTCAAGAGTG CACTGGCAAA ATTCAAGGAG 
GGGGCAGAGA TGGAGAGTTC AAAGGAAGAC AAGGCAAGGC AGGCTGAGGT AAAGCGGCTC 
TTCCGCCCCA TTGAGGAACT GAAGAAAGAC TTTGATGAGC TGAATGTTGT CATTGAGACT 
GACATGCAGA TCATGGTACG GCTGATCAAC AAGTTCAATA GTTCCAGCTC CAGTTTGGAA 
GAGAAGATTG CTGCGCTCTT TGATCTTGAA TATTATGTCC ATCAGATGGA CAATGCGCAG 
GACCTGCTTT CCTTTGGTGG TCTTCAAGTG GTGATCAATG GGCTGAACAG CACAGAGCCC 
CTCGTGAAGG AGTATGCTGC GTTTGTGCTG GGCGCTGCCT TTTCCAGCAA CCCCAAGGTC 
CAGGTGGAGG CCATCGAAGG GGGAGCCCTG CAGAAGCTGC TGGTCATCCT GGCCACGGAG 
CAGCCGCTCA CTGCAAAGAA GAAGGTCCTG TTTGCACTGT GCTCCCTGCT GCGCCACTTC 
CCCTATGCCC AGCGGCAGTT CCTGAAGCTC GGGGGGCTGC AGGTCCTGAG GACCCTGGTG 
CAGGAGAAGG GCACGGAGGT GCTCGCCGTG CGCGTGGTCA CACTGCTCTA CGACCTGGTC 
ACGGAGAAGA TGTTCGCCGA GGAGGAGGCT GAGCTGACCC AGGAGATGTC CCCAGAGAAG 
CTGCAGCAGT ATCGCCAGGT ACACCTCCTG CCAGGCCTGT GGGAACAGGG CTGGTGCGAG 
ATCACGGCCC ACCTCCTGGC GCTGCCCGAG CATGATGCCC GTGAGAAGGT GCTGCAGACA 
CTGGGCGTCC TCCTGACCAC CTGCCGGGAC CGCTACCGTC AGGACCCCCA GCTCGGCAGG 
ACACTGGCCA GCCTGCAGGC TGAGTACCAG GTGCTGGCCA GCCTGGAGCT GCAGGATGGT 
GAGGACGAGG GCTACTTCCA GGAGCTGCTG GGCTCTGTCA ACAGCTTGCT GAAGGAGCTG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071909.1
CDS	119..1504
Translation	MAPQSLPSSRMAPLGMLLGLLMAACFTFCLSHQNLKEFALTNPEKSSTKETERKETKAEEELDAEVLEVFHPTHEWQALQPGQAVPAGSHVRLNLQTGEREAKLQYEDKFRNNLKGKRLDINTNTYTSQDLKSALAKFKEGAEMESSKEDKARQAEVKRLFRPIEELKKDFDELNVVIETDMQIMVRLINKFNSSSSSLEEKIAALFDLEYYVHQMDNAQDLLSFGGLQVVINGLNSTEPLVKEYAAFVLGAAFSSNPKVQVEAIEGGALQKLLVILATEQPLTAKKKVLFALCSLLRHFPYAQRQFLKLGGLQVLRTLVQEKGTEVLAVRVVTLLYDLVTEKMFAEEEAELTQEMSPEKLQQYRQVHLLPGLWEQGWCEITAHLLALPEHDAREKVLQTLGVLLTTCRDRYRQDPQLGRTLASLQAEYQVLASLELQDGEDEGYFQELLGSVNSLLKELR

Target ORF information:

RefSeq Version	NM_022464.4
Organism	Homo sapiens(human)
Definition	Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022464.4

ORF Insert Sequence:

ATGGCTCCCC AGAGCCTGCC TTCATCTAGG ATGGCTCCTC TGGGCATGCT GCTTGGGCTG 
CTGATGGCCG CCTGCTTCAC CTTCTGCCTC AGTCATCAGA ACCTGAAGGA GTTTGCCCTG 
ACCAACCCAG AGAAGAGCAG CACCAAAGAA ACAGAGAGAA AAGAAACCAA AGCCGAGGAG 
GAGCTGGATG CCGAAGTCCT GGAGGTGTTC CACCCGACGC ATGAGTGGCA GGCCCTTCAG 
CCAGGGCAGG CTGTCCCTGC AGGATCCCAC GTACGGCTGA ATCTTCAGAC TGGGGAAAGA 
GAGGCAAAAC TCCAATATGA GGACAAGTTC CGAAATAATT TGAAAGGCAA AAGGCTGGAT 
ATCAACACCA ACACCTACAC ATCTCAGGAT CTCAAGAGTG CACTGGCAAA ATTCAAGGAG 
GGGGCAGAGA TGGAGAGTTC AAAGGAAGAC AAGGCAAGGC AGGCTGAGGT AAAGCGGCTC 
TTCCGCCCCA TTGAGGAACT GAAGAAAGAC TTTGATGAGC TGAATGTTGT CATTGAGACT 
GACATGCAGA TCATGGTACG GCTGATCAAC AAGTTCAATA GTTCCAGCTC CAGTTTGGAA 
GAGAAGATTG CTGCGCTCTT TGATCTTGAA TATTATGTCC ATCAGATGGA CAATGCGCAG 
GACCTGCTTT CCTTTGGTGG TCTTCAAGTG GTGATCAATG GGCTGAACAG CACAGAGCCC 
CTCGTGAAGG AGTATGCTGC GTTTGTGCTG GGCGCTGCCT TTTCCAGCAA CCCCAAGGTC 
CAGGTGGAGG CCATCGAAGG GGGAGCCCTG CAGAAGCTGC TGGTCATCCT GGCCACGGAG 
CAGCCGCTCA CTGCAAAGAA GAAGGTCCTG TTTGCACTGT GCTCCCTGCT GCGCCACTTC 
CCCTATGCCC AGCGGCAGTT CCTGAAGCTC GGGGGGCTGC AGGTCCTGAG GACCCTGGTG 
CAGGAGAAGG GCACGGAGGT GCTCGCCGTG CGCGTGGTCA CACTGCTCTA CGACCTGGTC 
ACGGAGAAGA TGTTCGCCGA GGAGGAGGCT GAGCTGACCC AGGAGATGTC CCCAGAGAAG 
CTGCAGCAGT ATCGCCAGGT ACACCTCCTG CCAGGCCTGT GGGAACAGGG CTGGTGCGAG 
ATCACGGCCC ACCTCCTGGC GCTGCCCGAG CATGATGCCC GTGAGAAGGT GCTGCAGACA 
CTGGGCGTCC TCCTGACCAC CTGCCGGGAC CGCTACCGTC AGGACCCCCA GCTCGGCAGG 
ACACTGGCCA GCCTGCAGGC TGAGTACCAG GTGCTGGCCA GCCTGGAGCT GCAGGATGGT 
GAGGACGAGG GCTACTTCCA GGAGCTGCTG GGCTCTGTCA ACAGCTTGCT GAAGGAGCTG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIL1 ( NM_022464.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_071909.1 Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 2, mRNA.

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CloneID	OHu23128
Clone ID Related Accession (Same CDS sequence)	NM_001037633.1 , NM_022464.4 , NM_022464.5 , NM_001037633.2 , XM_024446164.1
Accession Version	NM_022464.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Homo sapiens(human)
Product	nucleotide exchange factor SIL1 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK074624.1, BC011568.1, CD369479.1 and CA415266.1. On Nov 22, 2018 this sequence version replaced NM_022464.4. Transcript Variant: This variant (2) lacks an exon in the 5'UTR compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK074624.1, SRR1163655.658092.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000394817.7/ ENSP00000378294.2 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTCCCC AGAGCCTGCC TTCATCTAGG ATGGCTCCTC TGGGCATGCT GCTTGGGCTG 
CTGATGGCCG CCTGCTTCAC CTTCTGCCTC AGTCATCAGA ACCTGAAGGA GTTTGCCCTG 
ACCAACCCAG AGAAGAGCAG CACCAAAGAA ACAGAGAGAA AAGAAACCAA AGCCGAGGAG 
GAGCTGGATG CCGAAGTCCT GGAGGTGTTC CACCCGACGC ATGAGTGGCA GGCCCTTCAG 
CCAGGGCAGG CTGTCCCTGC AGGATCCCAC GTACGGCTGA ATCTTCAGAC TGGGGAAAGA 
GAGGCAAAAC TCCAATATGA GGACAAGTTC CGAAATAATT TGAAAGGCAA AAGGCTGGAT 
ATCAACACCA ACACCTACAC ATCTCAGGAT CTCAAGAGTG CACTGGCAAA ATTCAAGGAG 
GGGGCAGAGA TGGAGAGTTC AAAGGAAGAC AAGGCAAGGC AGGCTGAGGT AAAGCGGCTC 
TTCCGCCCCA TTGAGGAACT GAAGAAAGAC TTTGATGAGC TGAATGTTGT CATTGAGACT 
GACATGCAGA TCATGGTACG GCTGATCAAC AAGTTCAATA GTTCCAGCTC CAGTTTGGAA 
GAGAAGATTG CTGCGCTCTT TGATCTTGAA TATTATGTCC ATCAGATGGA CAATGCGCAG 
GACCTGCTTT CCTTTGGTGG TCTTCAAGTG GTGATCAATG GGCTGAACAG CACAGAGCCC 
CTCGTGAAGG AGTATGCTGC GTTTGTGCTG GGCGCTGCCT TTTCCAGCAA CCCCAAGGTC 
CAGGTGGAGG CCATCGAAGG GGGAGCCCTG CAGAAGCTGC TGGTCATCCT GGCCACGGAG 
CAGCCGCTCA CTGCAAAGAA GAAGGTCCTG TTTGCACTGT GCTCCCTGCT GCGCCACTTC 
CCCTATGCCC AGCGGCAGTT CCTGAAGCTC GGGGGGCTGC AGGTCCTGAG GACCCTGGTG 
CAGGAGAAGG GCACGGAGGT GCTCGCCGTG CGCGTGGTCA CACTGCTCTA CGACCTGGTC 
ACGGAGAAGA TGTTCGCCGA GGAGGAGGCT GAGCTGACCC AGGAGATGTC CCCAGAGAAG 
CTGCAGCAGT ATCGCCAGGT ACACCTCCTG CCAGGCCTGT GGGAACAGGG CTGGTGCGAG 
ATCACGGCCC ACCTCCTGGC GCTGCCCGAG CATGATGCCC GTGAGAAGGT GCTGCAGACA 
CTGGGCGTCC TCCTGACCAC CTGCCGGGAC CGCTACCGTC AGGACCCCCA GCTCGGCAGG 
ACACTGGCCA GCCTGCAGGC TGAGTACCAG GTGCTGGCCA GCCTGGAGCT GCAGGATGGT 
GAGGACGAGG GCTACTTCCA GGAGCTGCTG GGCTCTGTCA ACAGCTTGCT GAAGGAGCTG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071909.1
CDS	111..1496
Translation	MAPQSLPSSRMAPLGMLLGLLMAACFTFCLSHQNLKEFALTNPEKSSTKETERKETKAEEELDAEVLEVFHPTHEWQALQPGQAVPAGSHVRLNLQTGEREAKLQYEDKFRNNLKGKRLDINTNTYTSQDLKSALAKFKEGAEMESSKEDKARQAEVKRLFRPIEELKKDFDELNVVIETDMQIMVRLINKFNSSSSSLEEKIAALFDLEYYVHQMDNAQDLLSFGGLQVVINGLNSTEPLVKEYAAFVLGAAFSSNPKVQVEAIEGGALQKLLVILATEQPLTAKKKVLFALCSLLRHFPYAQRQFLKLGGLQVLRTLVQEKGTEVLAVRVVTLLYDLVTEKMFAEEEAELTQEMSPEKLQQYRQVHLLPGLWEQGWCEITAHLLALPEHDAREKVLQTLGVLLTTCRDRYRQDPQLGRTLASLQAEYQVLASLELQDGEDEGYFQELLGSVNSLLKELR

Target ORF information:

RefSeq Version	NM_022464.5
Organism	Homo sapiens(human)
Definition	Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022464.5

ORF Insert Sequence:

ATGGCTCCCC AGAGCCTGCC TTCATCTAGG ATGGCTCCTC TGGGCATGCT GCTTGGGCTG 
CTGATGGCCG CCTGCTTCAC CTTCTGCCTC AGTCATCAGA ACCTGAAGGA GTTTGCCCTG 
ACCAACCCAG AGAAGAGCAG CACCAAAGAA ACAGAGAGAA AAGAAACCAA AGCCGAGGAG 
GAGCTGGATG CCGAAGTCCT GGAGGTGTTC CACCCGACGC ATGAGTGGCA GGCCCTTCAG 
CCAGGGCAGG CTGTCCCTGC AGGATCCCAC GTACGGCTGA ATCTTCAGAC TGGGGAAAGA 
GAGGCAAAAC TCCAATATGA GGACAAGTTC CGAAATAATT TGAAAGGCAA AAGGCTGGAT 
ATCAACACCA ACACCTACAC ATCTCAGGAT CTCAAGAGTG CACTGGCAAA ATTCAAGGAG 
GGGGCAGAGA TGGAGAGTTC AAAGGAAGAC AAGGCAAGGC AGGCTGAGGT AAAGCGGCTC 
TTCCGCCCCA TTGAGGAACT GAAGAAAGAC TTTGATGAGC TGAATGTTGT CATTGAGACT 
GACATGCAGA TCATGGTACG GCTGATCAAC AAGTTCAATA GTTCCAGCTC CAGTTTGGAA 
GAGAAGATTG CTGCGCTCTT TGATCTTGAA TATTATGTCC ATCAGATGGA CAATGCGCAG 
GACCTGCTTT CCTTTGGTGG TCTTCAAGTG GTGATCAATG GGCTGAACAG CACAGAGCCC 
CTCGTGAAGG AGTATGCTGC GTTTGTGCTG GGCGCTGCCT TTTCCAGCAA CCCCAAGGTC 
CAGGTGGAGG CCATCGAAGG GGGAGCCCTG CAGAAGCTGC TGGTCATCCT GGCCACGGAG 
CAGCCGCTCA CTGCAAAGAA GAAGGTCCTG TTTGCACTGT GCTCCCTGCT GCGCCACTTC 
CCCTATGCCC AGCGGCAGTT CCTGAAGCTC GGGGGGCTGC AGGTCCTGAG GACCCTGGTG 
CAGGAGAAGG GCACGGAGGT GCTCGCCGTG CGCGTGGTCA CACTGCTCTA CGACCTGGTC 
ACGGAGAAGA TGTTCGCCGA GGAGGAGGCT GAGCTGACCC AGGAGATGTC CCCAGAGAAG 
CTGCAGCAGT ATCGCCAGGT ACACCTCCTG CCAGGCCTGT GGGAACAGGG CTGGTGCGAG 
ATCACGGCCC ACCTCCTGGC GCTGCCCGAG CATGATGCCC GTGAGAAGGT GCTGCAGACA 
CTGGGCGTCC TCCTGACCAC CTGCCGGGAC CGCTACCGTC AGGACCCCCA GCTCGGCAGG 
ACACTGGCCA GCCTGCAGGC TGAGTACCAG GTGCTGGCCA GCCTGGAGCT GCAGGATGGT 
GAGGACGAGG GCTACTTCCA GGAGCTGCTG GGCTCTGTCA ACAGCTTGCT GAAGGAGCTG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIL1 ( XM_024446164.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_024301932.1 Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant X2, mRNA.

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CloneID	OHu23128
Clone ID Related Accession (Same CDS sequence)	NM_001037633.1 , NM_022464.4 , NM_022464.5 , NM_001037633.2 , XM_024446164.1
Accession Version	XM_024446164.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1386bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nucleotide exchange factor SIL1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTCCCC AGAGCCTGCC TTCATCTAGG ATGGCTCCTC TGGGCATGCT GCTTGGGCTG 
CTGATGGCCG CCTGCTTCAC CTTCTGCCTC AGTCATCAGA ACCTGAAGGA GTTTGCCCTG 
ACCAACCCAG AGAAGAGCAG CACCAAAGAA ACAGAGAGAA AAGAAACCAA AGCCGAGGAG 
GAGCTGGATG CCGAAGTCCT GGAGGTGTTC CACCCGACGC ATGAGTGGCA GGCCCTTCAG 
CCAGGGCAGG CTGTCCCTGC AGGATCCCAC GTACGGCTGA ATCTTCAGAC TGGGGAAAGA 
GAGGCAAAAC TCCAATATGA GGACAAGTTC CGAAATAATT TGAAAGGCAA AAGGCTGGAT 
ATCAACACCA ACACCTACAC ATCTCAGGAT CTCAAGAGTG CACTGGCAAA ATTCAAGGAG 
GGGGCAGAGA TGGAGAGTTC AAAGGAAGAC AAGGCAAGGC AGGCTGAGGT AAAGCGGCTC 
TTCCGCCCCA TTGAGGAACT GAAGAAAGAC TTTGATGAGC TGAATGTTGT CATTGAGACT 
GACATGCAGA TCATGGTACG GCTGATCAAC AAGTTCAATA GTTCCAGCTC CAGTTTGGAA 
GAGAAGATTG CTGCGCTCTT TGATCTTGAA TATTATGTCC ATCAGATGGA CAATGCGCAG 
GACCTGCTTT CCTTTGGTGG TCTTCAAGTG GTGATCAATG GGCTGAACAG CACAGAGCCC 
CTCGTGAAGG AGTATGCTGC GTTTGTGCTG GGCGCTGCCT TTTCCAGCAA CCCCAAGGTC 
CAGGTGGAGG CCATCGAAGG GGGAGCCCTG CAGAAGCTGC TGGTCATCCT GGCCACGGAG 
CAGCCGCTCA CTGCAAAGAA GAAGGTCCTG TTTGCACTGT GCTCCCTGCT GCGCCACTTC 
CCCTATGCCC AGCGGCAGTT CCTGAAGCTC GGGGGGCTGC AGGTCCTGAG GACCCTGGTG 
CAGGAGAAGG GCACGGAGGT GCTCGCCGTG CGCGTGGTCA CACTGCTCTA CGACCTGGTC 
ACGGAGAAGA TGTTCGCCGA GGAGGAGGCT GAGCTGACCC AGGAGATGTC CCCAGAGAAG 
CTGCAGCAGT ATCGCCAGGT ACACCTCCTG CCAGGCCTGT GGGAACAGGG CTGGTGCGAG 
ATCACGGCCC ACCTCCTGGC GCTGCCCGAG CATGATGCCC GTGAGAAGGT GCTGCAGACA 
CTGGGCGTCC TCCTGACCAC CTGCCGGGAC CGCTACCGTC AGGACCCCCA GCTCGGCAGG 
ACACTGGCCA GCCTGCAGGC TGAGTACCAG GTGCTGGCCA GCCTGGAGCT GCAGGATGGT 
GAGGACGAGG GCTACTTCCA GGAGCTGCTG GGCTCTGTCA ACAGCTTGCT GAAGGAGCTG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024301932.1
CDS	144..1529
Translation	MAPQSLPSSRMAPLGMLLGLLMAACFTFCLSHQNLKEFALTNPEKSSTKETERKETKAEEELDAEVLEVFHPTHEWQALQPGQAVPAGSHVRLNLQTGEREAKLQYEDKFRNNLKGKRLDINTNTYTSQDLKSALAKFKEGAEMESSKEDKARQAEVKRLFRPIEELKKDFDELNVVIETDMQIMVRLINKFNSSSSSLEEKIAALFDLEYYVHQMDNAQDLLSFGGLQVVINGLNSTEPLVKEYAAFVLGAAFSSNPKVQVEAIEGGALQKLLVILATEQPLTAKKKVLFALCSLLRHFPYAQRQFLKLGGLQVLRTLVQEKGTEVLAVRVVTLLYDLVTEKMFAEEEAELTQEMSPEKLQQYRQVHLLPGLWEQGWCEITAHLLALPEHDAREKVLQTLGVLLTTCRDRYRQDPQLGRTLASLQAEYQVLASLELQDGEDEGYFQELLGSVNSLLKELR

Target ORF information:

RefSeq Version	XM_024446164.1
Organism	Homo sapiens(human)
Definition	Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024446164.1

ORF Insert Sequence:

ATGGCTCCCC AGAGCCTGCC TTCATCTAGG ATGGCTCCTC TGGGCATGCT GCTTGGGCTG 
CTGATGGCCG CCTGCTTCAC CTTCTGCCTC AGTCATCAGA ACCTGAAGGA GTTTGCCCTG 
ACCAACCCAG AGAAGAGCAG CACCAAAGAA ACAGAGAGAA AAGAAACCAA AGCCGAGGAG 
GAGCTGGATG CCGAAGTCCT GGAGGTGTTC CACCCGACGC ATGAGTGGCA GGCCCTTCAG 
CCAGGGCAGG CTGTCCCTGC AGGATCCCAC GTACGGCTGA ATCTTCAGAC TGGGGAAAGA 
GAGGCAAAAC TCCAATATGA GGACAAGTTC CGAAATAATT TGAAAGGCAA AAGGCTGGAT 
ATCAACACCA ACACCTACAC ATCTCAGGAT CTCAAGAGTG CACTGGCAAA ATTCAAGGAG 
GGGGCAGAGA TGGAGAGTTC AAAGGAAGAC AAGGCAAGGC AGGCTGAGGT AAAGCGGCTC 
TTCCGCCCCA TTGAGGAACT GAAGAAAGAC TTTGATGAGC TGAATGTTGT CATTGAGACT 
GACATGCAGA TCATGGTACG GCTGATCAAC AAGTTCAATA GTTCCAGCTC CAGTTTGGAA 
GAGAAGATTG CTGCGCTCTT TGATCTTGAA TATTATGTCC ATCAGATGGA CAATGCGCAG 
GACCTGCTTT CCTTTGGTGG TCTTCAAGTG GTGATCAATG GGCTGAACAG CACAGAGCCC 
CTCGTGAAGG AGTATGCTGC GTTTGTGCTG GGCGCTGCCT TTTCCAGCAA CCCCAAGGTC 
CAGGTGGAGG CCATCGAAGG GGGAGCCCTG CAGAAGCTGC TGGTCATCCT GGCCACGGAG 
CAGCCGCTCA CTGCAAAGAA GAAGGTCCTG TTTGCACTGT GCTCCCTGCT GCGCCACTTC 
CCCTATGCCC AGCGGCAGTT CCTGAAGCTC GGGGGGCTGC AGGTCCTGAG GACCCTGGTG 
CAGGAGAAGG GCACGGAGGT GCTCGCCGTG CGCGTGGTCA CACTGCTCTA CGACCTGGTC 
ACGGAGAAGA TGTTCGCCGA GGAGGAGGCT GAGCTGACCC AGGAGATGTC CCCAGAGAAG 
CTGCAGCAGT ATCGCCAGGT ACACCTCCTG CCAGGCCTGT GGGAACAGGG CTGGTGCGAG 
ATCACGGCCC ACCTCCTGGC GCTGCCCGAG CATGATGCCC GTGAGAAGGT GCTGCAGACA 
CTGGGCGTCC TCCTGACCAC CTGCCGGGAC CGCTACCGTC AGGACCCCCA GCTCGGCAGG 
ACACTGGCCA GCCTGCAGGC TGAGTACCAG GTGCTGGCCA GCCTGGAGCT GCAGGATGGT 
GAGGACGAGG GCTACTTCCA GGAGCTGCTG GGCTCTGTCA ACAGCTTGCT GAAGGAGCTG 
AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SIL1 ( XM_011543570.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011541872.1 Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant X1, mRNA.

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CloneID	OHu39670
Clone ID Related Accession (Same CDS sequence)	XM_011543570.2
Accession Version	XM_011543570.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	nucleotide exchange factor SIL1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000005.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011543570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGGAAA TGTCTGACAT GTCCCTGACT ATGGCTCCCC AGAGCCTGCC TTCATCTAGG 
ATGGCTCCTC TGGGCATGCT GCTTGGGCTG CTGATGGCCG CCTGCTTCAC CTTCTGCCTC 
AGTCATCAGA ACCTGAAGGA GTTTGCCCTG ACCAACCCAG AGAAGAGCAG CACCAAAGAA 
ACAGAGAGAA AAGAAACCAA AGCCGAGGAG GAGCTGGATG CCGAAGTCCT GGAGGTGTTC 
CACCCGACGC ATGAGTGGCA GGCCCTTCAG CCAGGGCAGG CTGTCCCTGC AGGATCCCAC 
GTACGGCTGA ATCTTCAGAC TGGGGAAAGA GAGGCAAAAC TCCAATATGA GGACAAGTTC 
CGAAATAATT TGAAAGGCAA AAGGCTGGAT ATCAACACCA ACACCTACAC ATCTCAGGAT 
CTCAAGAGTG CACTGGCAAA ATTCAAGGAG GGGGCAGAGA TGGAGAGTTC AAAGGAAGAC 
AAGGCAAGGC AGGCTGAGGT AAAGCGGCTC TTCCGCCCCA TTGAGGAACT GAAGAAAGAC 
TTTGATGAGC TGAATGTTGT CATTGAGACT GACATGCAGA TCATGGTACG GCTGATCAAC 
AAGTTCAATA GTTCCAGCTC CAGTTTGGAA GAGAAGATTG CTGCGCTCTT TGATCTTGAA 
TATTATGTCC ATCAGATGGA CAATGCGCAG GACCTGCTTT CCTTTGGTGG TCTTCAAGTG 
GTGATCAATG GGCTGAACAG CACAGAGCCC CTCGTGAAGG AGTATGCTGC GTTTGTGCTG 
GGCGCTGCCT TTTCCAGCAA CCCCAAGGTC CAGGTGGAGG CCATCGAAGG GGGAGCCCTG 
CAGAAGCTGC TGGTCATCCT GGCCACGGAG CAGCCGCTCA CTGCAAAGAA GAAGGTCCTG 
TTTGCACTGT GCTCCCTGCT GCGCCACTTC CCCTATGCCC AGCGGCAGTT CCTGAAGCTC 
GGGGGGCTGC AGGTCCTGAG GACCCTGGTG CAGGAGAAGG GCACGGAGGT GCTCGCCGTG 
CGCGTGGTCA CACTGCTCTA CGACCTGGTC ACGGAGAAGA TGTTCGCCGA GGAGGAGGCT 
GAGCTGACCC AGGAGATGTC CCCAGAGAAG CTGCAGCAGT ATCGCCAGGT ACACCTCCTG 
CCAGGCCTGT GGGAACAGGG CTGGTGCGAG ATCACGGCCC ACCTCCTGGC GCTGCCCGAG 
CATGATGCCC GTGAGAAGGT GCTGCAGACA CTGGGCGTCC TCCTGACCAC CTGCCGGGAC 
CGCTACCGTC AGGACCCCCA GCTCGGCAGG ACACTGGCCA GCCTGCAGGC TGAGTACCAG 
GTGCTGGCCA GCCTGGAGCT GCAGGATGGT GAGGACGAGG GCTACTTCCA GGAGCTGCTG 
GGCTCTGTCA ACAGCTTGCT GAAGGAGCTG AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011541872.1
CDS	214..1629
Translation	MQEMSDMSLTMAPQSLPSSRMAPLGMLLGLLMAACFTFCLSHQNLKEFALTNPEKSSTKETERKETKAEEELDAEVLEVFHPTHEWQALQPGQAVPAGSHVRLNLQTGEREAKLQYEDKFRNNLKGKRLDINTNTYTSQDLKSALAKFKEGAEMESSKEDKARQAEVKRLFRPIEELKKDFDELNVVIETDMQIMVRLINKFNSSSSSLEEKIAALFDLEYYVHQMDNAQDLLSFGGLQVVINGLNSTEPLVKEYAAFVLGAAFSSNPKVQVEAIEGGALQKLLVILATEQPLTAKKKVLFALCSLLRHFPYAQRQFLKLGGLQVLRTLVQEKGTEVLAVRVVTLLYDLVTEKMFAEEEAELTQEMSPEKLQQYRQVHLLPGLWEQGWCEITAHLLALPEHDAREKVLQTLGVLLTTCRDRYRQDPQLGRTLASLQAEYQVLASLELQDGEDEGYFQELLGSVNSLLKELR

Target ORF information:

RefSeq Version	XM_011543570.2
Organism	Homo sapiens(human)
Definition	Homo sapiens SIL1 nucleotide exchange factor (SIL1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011543570.2

ORF Insert Sequence:

ATGCAGGAAA TGTCTGACAT GTCCCTGACT ATGGCTCCCC AGAGCCTGCC TTCATCTAGG 
ATGGCTCCTC TGGGCATGCT GCTTGGGCTG CTGATGGCCG CCTGCTTCAC CTTCTGCCTC 
AGTCATCAGA ACCTGAAGGA GTTTGCCCTG ACCAACCCAG AGAAGAGCAG CACCAAAGAA 
ACAGAGAGAA AAGAAACCAA AGCCGAGGAG GAGCTGGATG CCGAAGTCCT GGAGGTGTTC 
CACCCGACGC ATGAGTGGCA GGCCCTTCAG CCAGGGCAGG CTGTCCCTGC AGGATCCCAC 
GTACGGCTGA ATCTTCAGAC TGGGGAAAGA GAGGCAAAAC TCCAATATGA GGACAAGTTC 
CGAAATAATT TGAAAGGCAA AAGGCTGGAT ATCAACACCA ACACCTACAC ATCTCAGGAT 
CTCAAGAGTG CACTGGCAAA ATTCAAGGAG GGGGCAGAGA TGGAGAGTTC AAAGGAAGAC 
AAGGCAAGGC AGGCTGAGGT AAAGCGGCTC TTCCGCCCCA TTGAGGAACT GAAGAAAGAC 
TTTGATGAGC TGAATGTTGT CATTGAGACT GACATGCAGA TCATGGTACG GCTGATCAAC 
AAGTTCAATA GTTCCAGCTC CAGTTTGGAA GAGAAGATTG CTGCGCTCTT TGATCTTGAA 
TATTATGTCC ATCAGATGGA CAATGCGCAG GACCTGCTTT CCTTTGGTGG TCTTCAAGTG 
GTGATCAATG GGCTGAACAG CACAGAGCCC CTCGTGAAGG AGTATGCTGC GTTTGTGCTG 
GGCGCTGCCT TTTCCAGCAA CCCCAAGGTC CAGGTGGAGG CCATCGAAGG GGGAGCCCTG 
CAGAAGCTGC TGGTCATCCT GGCCACGGAG CAGCCGCTCA CTGCAAAGAA GAAGGTCCTG 
TTTGCACTGT GCTCCCTGCT GCGCCACTTC CCCTATGCCC AGCGGCAGTT CCTGAAGCTC 
GGGGGGCTGC AGGTCCTGAG GACCCTGGTG CAGGAGAAGG GCACGGAGGT GCTCGCCGTG 
CGCGTGGTCA CACTGCTCTA CGACCTGGTC ACGGAGAAGA TGTTCGCCGA GGAGGAGGCT 
GAGCTGACCC AGGAGATGTC CCCAGAGAAG CTGCAGCAGT ATCGCCAGGT ACACCTCCTG 
CCAGGCCTGT GGGAACAGGG CTGGTGCGAG ATCACGGCCC ACCTCCTGGC GCTGCCCGAG 
CATGATGCCC GTGAGAAGGT GCTGCAGACA CTGGGCGTCC TCCTGACCAC CTGCCGGGAC 
CGCTACCGTC AGGACCCCCA GCTCGGCAGG ACACTGGCCA GCCTGCAGGC TGAGTACCAG 
GTGCTGGCCA GCCTGGAGCT GCAGGATGGT GAGGACGAGG GCTACTTCCA GGAGCTGCTG 
GGCTCTGTCA ACAGCTTGCT GAAGGAGCTG AGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Bap (Sil1) regulates the molecular chaperone BiP by coupling release of nucleotide and substrate.
Nature structural & molecular biology25(1)90-100(2018 Jan)
Rosam M,Krader D,Nickels C,Hochmair J,Back KC,Agam G,Barth A,Zeymer C,Hendrix J,Schneider M,Antes I,Reinstein J,Lamb DC,Buchner J

Novel SIL1 nonstop mutation in a Chinese consanguineous family with Marinesco-Sj?gren syndrome and Dandy-Walker syndrome.
Clinica chimica acta; international journal of clinical chemistry4581-4(2016 Jul)
Gai N,Jiang C,Zou YY,Zheng Y,Liang DS,Wu LQ

Marinesco-Sj?gren Syndrome in an Emirati Child with a Novel Mutation in SIL1 Affecting the 5' Untranslated Region.
Medical principles and practice : international journal of the Kuwait University, Health Science Centre25(6)580-582(2016)
Nair P,Hamzeh AR,Mohamed M,Tawfiq N,Al-Ali MT,Bastaki F

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SIL1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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