EDDM3B cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	EDDM3B
Entrez Gene ID	64184
Full Name	epididymal protein 3B
Synonyms	EP3B,FAM12B,HE3-BETA,HE3B,RAM2
General protein information	Preferred Names epididymal protein 3B Names epididymal secretory protein E3-beta family with sequence similarity 12, member B (epididymal) human epididymis-specific 3 beta human epididymis-specific protein 3-beta ribonuclease A M2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q11.2
Summary	Testicular sperm are morphologically differentiated but are not progressively motile nor able to fertilize an egg. Post-testicular maturation requires exposure of spermatozoa to the microenvironment of the epididymal lumen. Spermatozoa undergo extensive changes in the epididymis, including enzymatic modifications, loss of pre-existing components and addition of new glycoproteins from epididymal secretions. These modifying proteins and enzymes are synthesized by epithelial cells lining the epididymal duct and secreted apically into the lumen, where they come into contact with, and may be absorbed onto, the sperm membranes. The proteins encoded by the genes in this cluster are synthesized and secreted by epididymal epithelial cells. [provided by RefSeq, Jul 2008].
Disorder MIM:	611582

mRNA and Protein(s)

mRNA	Protein	Name
NM_022360.5	NP_071755.1	epididymal secretory protein E3-beta precursor
NM_022360.4	NP_071755.1	epididymal secretory protein E3-beta precursor

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Eddm3b	NP_835204.1
Pan troglodytes (chimpanzee)	EDDM3B	XP_001142430.1
Mus musculus (house mouse)	Eddm3b	NP_987104.1
Homo sapiens (human)	EDDM3B	NP_071755.1
	EDDM3B	XP_001092129.1
Canis lupus familiaris (dog)	EDDM3B	XP_003432021.1
Bos taurus (cattle)	LOC100297646	XP_002690787.1

Gene Ontology
Bibliography

Related articles in PubMed

Comparative genomic analysis of eutherian ribonuclease A genes.
Premzl M
Molecular genetics and genomics : MGG289(2)161-7(2014 Apr)

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Clark HF, Gurney AL, Abaya E, Baker K, Baldwin D, Brush J, Chen J, Chow B, Chui C, Crowley C, Currell B, Deuel B, Dowd P, Eaton D, Foster J, Grimaldi C, Gu Q, Hass PE, Heldens S, Huang A, Kim HS, Klimowski L, Jin Y, Johnson S, Lee J, Lewis L, Liao D, Mark M, Robbie E, Sanchez C, Schoenfeld J, Seshagiri S, Simmons L, Singh J, Smith V, Stinson J, Vagts A, Vandlen R, Watanabe C, Wieand D, Woods K, Xie MH, Yansura D, Yi S, Yu G, Yuan J, Zhang M, Zhang Z, Goddard A, Wood WI, Godowski P, Gray A
Genome research13(10)2265-70(2003 Oct)

Human epididymal proteins and sperm function during fertilization: un update.
Lasserre A, Barrozo R, Tez?n JG, Miranda PV, Vazquez-Levin MH
Biological research34(3-4)165-78(2001)

Function of human epididymal proteins in sperm maturation.
Kirchhoff C, Osterhoff C, Pera I, Schr?ter S
Andrologia30(4-5)225-32(1998 Aug-Sep)

Molecular characterization of epididymal proteins.
Kirchhoff C
Reviews of reproduction3(2)86-95(1998 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following EDDM3B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the EDDM3B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu29212	NM_022360.5 Latest version!	Homo sapiens epididymal protein 3B (EDDM3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu29212	NM_022360.4	Homo sapiens epididymal protein 3B (EDDM3B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

EDDM3B ( NM_022360.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_071755.1 Homo sapiens epididymal protein 3B (EDDM3B), mRNA.

Price & Availability↑

CloneID	OHu29212
Clone ID Related Accession (Same CDS sequence)	NM_022360.5 , NM_022360.4
Accession Version	NM_022360.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	epididymal secretory protein E3-beta precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL163636.7, AY358865.1, BX112699.1 and AI075915.1. On May 17, 2019 this sequence version replaced NM_022360.4. ##Evidence-Data-START## Transcript exon combination :: AY358865.1, SRR5189652.104446.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267784 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000326783.4/ ENSP00000314810.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCATCGT CTCTAAAGAT CTGGGGCACA CTCTTGGCCC TACTTTGCAT CCTATGCACA 
CTGCTTGTAC AGAGCAAAGA AGTTTCTTGG AGAGAATTCA TGAAACAGCA CTACTTAAGT 
CCAAGTCGAG AATTCAGAGA GTACAAATGT GATGTCCTCA TGAGAGAAAA TGAAGCTCTG 
AAAGACAAGA GCTCTCACAT GTTTATCTAT ATCTCATGGT ACAAAATCGA GCATATATGC 
ACTAGTGACA ACTGGATGGA TCGCTTCCGA AATGCATATG TATGGGTCCA GAATCCTCTC 
AAAGTACTCA AGTGTCACCA GGAGAATTCC AAAAATAGCT ACACAGAGAG CAGGAGCTTC 
AACTACATTG AATTCCATTG TAGCATGGAC GGGTATGTTG ATAGCATAGA AGACCTAAAG 
ATGGTAGAAC CTATCGGCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071755.1
CDS	122..565
Translation	MASSLKIWGTLLALLCILCTLLVQSKEVSWREFMKQHYLSPSREFREYKCDVLMRENEALKDKSSHMFIYISWYKIEHICTSDNWMDRFRNAYVWVQNPLKVLKCHQENSKNSYTESRSFNYIEFHCSMDGYVDSIEDLKMVEPIGN

Target ORF information:

RefSeq Version	NM_022360.5
Organism	Homo sapiens(human)
Definition	Homo sapiens epididymal protein 3B (EDDM3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022360.5

ORF Insert Sequence:

ATGGCATCGT CTCTAAAGAT CTGGGGCACA CTCTTGGCCC TACTTTGCAT CCTATGCACA 
CTGCTTGTAC AGAGCAAAGA AGTTTCTTGG AGAGAATTCA TGAAACAGCA CTACTTAAGT 
CCAAGTCGAG AATTCAGAGA GTACAAATGT GATGTCCTCA TGAGAGAAAA TGAAGCTCTG 
AAAGACAAGA GCTCTCACAT GTTTATCTAT ATCTCATGGT ACAAAATCGA GCATATATGC 
ACTAGTGACA ACTGGATGGA TCGCTTCCGA AATGCATATG TATGGGTCCA GAATCCTCTC 
AAAGTACTCA AGTGTCACCA GGAGAATTCC AAAAATAGCT ACACAGAGAG CAGGAGCTTC 
AACTACATTG AATTCCATTG TAGCATGGAC GGGTATGTTG ATAGCATAGA AGACCTAAAG 
ATGGTAGAAC CTATCGGCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

EDDM3B ( NM_022360.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_071755.1 Homo sapiens epididymal protein 3B (EDDM3B), mRNA.

Price & Availability↑

CloneID	OHu29212
Clone ID Related Accession (Same CDS sequence)	NM_022360.5 , NM_022360.4
Accession Version	NM_022360.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 444bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-22
Organism	Homo sapiens(human)
Product	epididymal secretory protein E3-beta precursor
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AY358865.1, BX112699.1 and AI075915.1. On Apr 6, 2006 this sequence version replaced NM_022360.3. ##Evidence-Data-START## Transcript exon combination :: AY358865.1, X76386.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03267784 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCATCGT CTCTAAAGAT CTGGGGCACA CTCTTGGCCC TACTTTGCAT CCTATGCACA 
CTGCTTGTAC AGAGCAAAGA AGTTTCTTGG AGAGAATTCA TGAAACAGCA CTACTTAAGT 
CCAAGTCGAG AATTCAGAGA GTACAAATGT GATGTCCTCA TGAGAGAAAA TGAAGCTCTG 
AAAGACAAGA GCTCTCACAT GTTTATCTAT ATCTCATGGT ACAAAATCGA GCATATATGC 
ACTAGTGACA ACTGGATGGA TCGCTTCCGA AATGCATATG TATGGGTCCA GAATCCTCTC 
AAAGTACTCA AGTGTCACCA GGAGAATTCC AAAAATAGCT ACACAGAGAG CAGGAGCTTC 
AACTACATTG AATTCCATTG TAGCATGGAC GGGTATGTTG ATAGCATAGA AGACCTAAAG 
ATGGTAGAAC CTATCGGCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071755.1
CDS	99..542
Translation	MASSLKIWGTLLALLCILCTLLVQSKEVSWREFMKQHYLSPSREFREYKCDVLMRENEALKDKSSHMFIYISWYKIEHICTSDNWMDRFRNAYVWVQNPLKVLKCHQENSKNSYTESRSFNYIEFHCSMDGYVDSIEDLKMVEPIGN

Target ORF information:

RefSeq Version	NM_022360.4
Organism	Homo sapiens(human)
Definition	Homo sapiens epididymal protein 3B (EDDM3B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022360.4

ORF Insert Sequence:

ATGGCATCGT CTCTAAAGAT CTGGGGCACA CTCTTGGCCC TACTTTGCAT CCTATGCACA 
CTGCTTGTAC AGAGCAAAGA AGTTTCTTGG AGAGAATTCA TGAAACAGCA CTACTTAAGT 
CCAAGTCGAG AATTCAGAGA GTACAAATGT GATGTCCTCA TGAGAGAAAA TGAAGCTCTG 
AAAGACAAGA GCTCTCACAT GTTTATCTAT ATCTCATGGT ACAAAATCGA GCATATATGC 
ACTAGTGACA ACTGGATGGA TCGCTTCCGA AATGCATATG TATGGGTCCA GAATCCTCTC 
AAAGTACTCA AGTGTCACCA GGAGAATTCC AAAAATAGCT ACACAGAGAG CAGGAGCTTC 
AACTACATTG AATTCCATTG TAGCATGGAC GGGTATGTTG ATAGCATAGA AGACCTAAAG 
ATGGTAGAAC CTATCGGCAA CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Comparative genomic analysis of eutherian ribonuclease A genes.
Molecular genetics and genomics : MGG289(2)161-7(2014 Apr)
Premzl M

The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment.
Genome research13(10)2265-70(2003 Oct)
Clark HF,Gurney AL,Abaya E,Baker K,Baldwin D,Brush J,Chen J,Chow B,Chui C,Crowley C,Currell B,Deuel B,Dowd P,Eaton D,Foster J,Grimaldi C,Gu Q,Hass PE,Heldens S,Huang A,Kim HS,Klimowski L,Jin Y,Johnson S,Lee J,Lewis L,Liao D,Mark M,Robbie E,Sanchez C,Schoenfeld J,Seshagiri S,Simmons L,Singh J,Smith V,Stinson J,Vagts A,Vandlen R,Watanabe C,Wieand D,Woods K,Xie MH,Yansura D,Yi S,Yu G,Yuan J,Zhang M,Zhang Z,Goddard A,Wood WI,Godowski P,Gray A

Human epididymal proteins and sperm function during fertilization: un update.
Biological research34(3-4)165-78(2001)
Lasserre A,Barrozo R,Tez?n JG,Miranda PV,Vazquez-Levin MH

Function of human epididymal proteins in sperm maturation.
Andrologia30(4-5)225-32(1998 Aug-Sep)
Kirchhoff C,Osterhoff C,Pera I,Schr?ter S

Molecular characterization of epididymal proteins.
Reviews of reproduction3(2)86-95(1998 May)
Kirchhoff C

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EDDM3B cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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