PDF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PDF
Entrez Gene ID	64146
Full Name	peptide deformylase, mitochondrial
General protein information	Preferred Names peptide deformylase, mitochondrial Names peptide deformylase, mitochondrial peptide deformylase-like protein polypeptide deformylase
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q22.1
Summary	Protein synthesis proceeds after formylation of methionine by methionyl-tRNA formyl transferase (FMT) and transfer of the charged initiator f-met tRNA to the ribosome. In eubacteria and eukaryotic organelles the product of this gene, peptide deformylase (PDF), removes the formyl group from the initiating methionine of nascent peptides. In eubacteria, deformylation of nascent peptides is required for subsequent cleavage of initiating methionines by methionine aminopeptidase. The discovery that a natural inhibitor of PDF, actinonin, acts as an antimicrobial agent in some bacteria has spurred intensive research into the design of bacterial-specific PDF inhibitors. In human cells, only mitochondrial proteins have N-formylation of initiating methionines. Protein inhibitors of PDF or siRNAs of PDF block the growth of cancer cell lines but have no effect on normal cell growth. In humans, PDF function may therefore be restricted to rapidly growing cells. [provided by RefSeq, Nov 2008].

mRNA and Protein(s)

mRNA	Protein	Name
NM_022341.2	NP_071736.1	peptide deformylase, mitochondrial precursor
NM_022341.1	NP_071736.1	peptide deformylase, mitochondrial precursor

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDF	NP_071736.1
Canis lupus familiaris (dog)	PDF	XP_853641.1
Xenopus tropicalis (tropical clawed frog)	pdf	NP_001004979.1
Mus musculus (house mouse)	Pdf	NP_080789.2
Drosophila melanogaster (fruit fly)	CG31278	NP_731494.1
Oryza sativa (rice)	Os01g0555800	NP_001043323.1
Rattus norvegicus (Norway rat)	Pdf	XP_001073696.3
Danio rerio (zebrafish)	pdf	NP_001028902.1
	PDF	XP_002802550.1
Bos taurus (cattle)	PDF	XP_002694953.1
Arabidopsis thaliana (thale cress)	PDF1A	NP_563974.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003861	XP_310421.4

Gene Ontology
Bibliography

Related articles in PubMed

Structure-Based Drug Design of Small Molecule Peptide Deformylase Inhibitors to Treat Cancer.
Gao J, Wang T, Qiu S, Zhu Y, Liang L, Zheng Y
Molecules (Basel, Switzerland)21(4)396(2016 Mar)

Inhibition of human mitochondrial peptide deformylase causes apoptosis in c-myc-overexpressing hematopoietic cancers.
Sheth A, Escobar-Alvarez S, Gardner J, Ran L, Heaney ML, Scheinberg DA
Cell death & disease5e1152(2014 Mar)

Overexpression of peptide deformylase in breast, colon, and lung cancers.
Randhawa H, Chikara S, Gehring D, Yildirim T, Menon J, Reindl KM
BMC cancer13321(2013 Jul)

Transcriptional regulation of the human mitochondrial peptide deformylase (PDF).
Pereira-Castro I, Costa LT, Amorim A, Azevedo L
Biochemical and biophysical research communications421(4)825-31(2012 May)

Successful COG8 and PDF overlap is mediated by alterations in splicing and polyadenylation signals.
Pereira-Castro I, Quental R, da Costa LT, Amorim A, Azevedo L
Human genetics131(2)265-74(2012 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

compound M7594_0037 exhibited potent anticancer activities against HeLa, A549 and MCF-7 cell lines with IC50s of 35.26, 29.63 and 24.63 muM, respectively. Molecular docking studies suggested that M7594_0037 and its three derivatives could interact with Human peptide deformylase by several conserved hydrogen bonds.
Title: Structure-Based Drug Design of Small Molecule Peptide Deformylase Inhibitors to Treat Cancer.

c-myc regulated the expression of human PDF.
Title: Inhibition of human mitochondrial peptide deformylase causes apoptosis in c-myc-overexpressing hematopoietic cancers.

This is the first report showing that PDF is over-expressed in breast, colon, and lung cancers
Title: Overexpression of peptide deformylase in breast, colon, and lung cancers.

these data reveals that PDF has several transcription start sites, the most important of which only 18 bp from the initiation codon.
Title: Transcriptional regulation of the human mitochondrial peptide deformylase (PDF).

the origin, evolution and preservation of the COG8/PDF same-strand overlap follow similar mechanistic steps as those documented for antisense overlaps where gain and/or loss of splice sites and polyadenylation signals seems to drive the process.
Title: Successful COG8 and PDF overlap is mediated by alterations in splicing and polyadenylation signals.

The following PDF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PDF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18778	NM_022341.2 Latest version!	Homo sapiens peptide deformylase, mitochondrial (PDF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu18778	NM_022341.1	Homo sapiens peptide deformylase, mitochondrial (PDF), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PDF ( NM_022341.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_071736.1 Homo sapiens peptide deformylase, mitochondrial (PDF), mRNA.

Price & Availability↑

CloneID	OHu18778
Clone ID Related Accession (Same CDS sequence)	NM_022341.2 , NM_022341.1
Accession Version	NM_022341.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	peptide deformylase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF239156.1 and AC026464.7. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_022341.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.381020.1, AF239156.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology MANE Ensembl match :: ENST00000288022.2/ ENSP00000288022.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCCCGGC TGTGGGGCGC GCTGAGTCTT TGGCCACTGT GGGCGGCCGT GCCGTGGGGC 
GGGGCGGCAG CCGTCGGTGT CCGGGCTTGC AGCTCCACGG CCGCCCCGGA CGGCGTCGAG 
GGCCCGGCGC TGCGGCGCTC CTATTGGCGC CACCTGAGGC GTCTGGTGCT GGGTCCTCCC 
GAACCGCCGT TCTCGCACGT GTGCCAAGTC GGGGACCCGG TGCTGCGCGG CGTGGCGGCC 
CCGGTGGAGC GGGCGCAGCT AGGCGGGCCC GAGCTGCAGC GGCTGACGCA ACGGCTGGTC 
CAGGTGATGC GGCGGCGGCG CTGCGTGGGC CTAAGCGCGC CGCAGCTGGG GGTGCCGCGG 
CAGGTGCTGG CGCTGGAGCT CCCCGAGGCG CTGTGTCGGG AGTGCCCGCC CCGCCAGCGC 
GCGCTCCGCC AAATGGAGCC CTTCCCCCTG CGCGTGTTCG TGAACCCCAG CCTGCGAGTG 
CTTGACAGCC GCCTGGTCAC CTTTCCCGAG GGCTGCGAGA GCGTCGCCGG CTTCCTGGCC 
TGCGTGCCCC GCTTCCAGGC GGTGCAGATC TCAGGGCTGG ACCCCAATGG AGAACAGGTG 
GTGTGGCAGG CGAGCGGGTG GGCAGCCCGC ATCATCCAGC ACGAGATGGA CCACCTGCAG 
GGCTGCCTGT TTATTGACAA AATGGACAGC AGGACGTTCA CAAACGTCTA TTGGATGAAG 
GTGAATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071736.1
CDS	19..750
Translation	MARLWGALSLWPLWAAVPWGGAAAVGVRACSSTAAPDGVEGPALRRSYWRHLRRLVLGPPEPPFSHVCQVGDPVLRGVAAPVERAQLGGPELQRLTQRLVQVMRRRRCVGLSAPQLGVPRQVLALELPEALCRECPPRQRALRQMEPFPLRVFVNPSLRVLDSRLVTFPEGCESVAGFLACVPRFQAVQISGLDPNGEQVVWQASGWAARIIQHEMDHLQGCLFIDKMDSRTFTNVYWMKVND

Target ORF information:

RefSeq Version	NM_022341.2
Organism	Homo sapiens(human)
Definition	Homo sapiens peptide deformylase, mitochondrial (PDF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022341.2

ORF Insert Sequence:

ATGGCCCGGC TGTGGGGCGC GCTGAGTCTT TGGCCACTGT GGGCGGCCGT GCCGTGGGGC 
GGGGCGGCAG CCGTCGGTGT CCGGGCTTGC AGCTCCACGG CCGCCCCGGA CGGCGTCGAG 
GGCCCGGCGC TGCGGCGCTC CTATTGGCGC CACCTGAGGC GTCTGGTGCT GGGTCCTCCC 
GAACCGCCGT TCTCGCACGT GTGCCAAGTC GGGGACCCGG TGCTGCGCGG CGTGGCGGCC 
CCGGTGGAGC GGGCGCAGCT AGGCGGGCCC GAGCTGCAGC GGCTGACGCA ACGGCTGGTC 
CAGGTGATGC GGCGGCGGCG CTGCGTGGGC CTAAGCGCGC CGCAGCTGGG GGTGCCGCGG 
CAGGTGCTGG CGCTGGAGCT CCCCGAGGCG CTGTGTCGGG AGTGCCCGCC CCGCCAGCGC 
GCGCTCCGCC AAATGGAGCC CTTCCCCCTG CGCGTGTTCG TGAACCCCAG CCTGCGAGTG 
CTTGACAGCC GCCTGGTCAC CTTTCCCGAG GGCTGCGAGA GCGTCGCCGG CTTCCTGGCC 
TGCGTGCCCC GCTTCCAGGC GGTGCAGATC TCAGGGCTGG ACCCCAATGG AGAACAGGTG 
GTGTGGCAGG CGAGCGGGTG GGCAGCCCGC ATCATCCAGC ACGAGATGGA CCACCTGCAG 
GGCTGCCTGT TTATTGACAA AATGGACAGC AGGACGTTCA CAAACGTCTA TTGGATGAAG 
GTGAATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PDF ( NM_022341.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_071736.1 Homo sapiens peptide deformylase, mitochondrial (PDF), mRNA.

Price & Availability↑

CloneID	OHu18778
Clone ID Related Accession (Same CDS sequence)	NM_022341.2 , NM_022341.1
Accession Version	NM_022341.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 732bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	peptide deformylase, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF239156.1. This sequence is a reference standard in the RefSeqGene project. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF239156.1, SRR1163658.237831.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: inferred from homology ##RefSeq-Attributes-END##

ATGGCCCGGC TGTGGGGCGC GCTGAGTCTT TGGCCACTGT GGGCGGCCGT GCCGTGGGGC 
GGGGCGGCAG CCGTCGGTGT CCGGGCTTGC AGCTCCACGG CCGCCCCGGA CGGCGTCGAG 
GGCCCGGCGC TGCGGCGCTC CTATTGGCGC CACCTGAGGC GTCTGGTGCT GGGTCCTCCC 
GAACCGCCGT TCTCGCACGT GTGCCAAGTC GGGGACCCGG TGCTGCGCGG CGTGGCGGCC 
CCGGTGGAGC GGGCGCAGCT AGGCGGGCCC GAGCTGCAGC GGCTGACGCA ACGGCTGGTC 
CAGGTGATGC GGCGGCGGCG CTGCGTGGGC CTAAGCGCGC CGCAGCTGGG GGTGCCGCGG 
CAGGTGCTGG CGCTGGAGCT CCCCGAGGCG CTGTGTCGGG AGTGCCCGCC CCGCCAGCGC 
GCGCTCCGCC AAATGGAGCC CTTCCCCCTG CGCGTGTTCG TGAACCCCAG CCTGCGAGTG 
CTTGACAGCC GCCTGGTCAC CTTTCCCGAG GGCTGCGAGA GCGTCGCCGG CTTCCTGGCC 
TGCGTGCCCC GCTTCCAGGC GGTGCAGATC TCAGGGCTGG ACCCCAATGG AGAACAGGTG 
GTGTGGCAGG CGAGCGGGTG GGCAGCCCGC ATCATCCAGC ACGAGATGGA CCACCTGCAG 
GGCTGCCTGT TTATTGACAA AATGGACAGC AGGACGTTCA CAAACGTCTA TTGGATGAAG 
GTGAATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071736.1
CDS	26..757
Translation	MARLWGALSLWPLWAAVPWGGAAAVGVRACSSTAAPDGVEGPALRRSYWRHLRRLVLGPPEPPFSHVCQVGDPVLRGVAAPVERAQLGGPELQRLTQRLVQVMRRRRCVGLSAPQLGVPRQVLALELPEALCRECPPRQRALRQMEPFPLRVFVNPSLRVLDSRLVTFPEGCESVAGFLACVPRFQAVQISGLDPNGEQVVWQASGWAARIIQHEMDHLQGCLFIDKMDSRTFTNVYWMKVND

Target ORF information:

RefSeq Version	NM_022341.1
Organism	Homo sapiens(human)
Definition	Homo sapiens peptide deformylase, mitochondrial (PDF), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022341.1

ORF Insert Sequence:

ATGGCCCGGC TGTGGGGCGC GCTGAGTCTT TGGCCACTGT GGGCGGCCGT GCCGTGGGGC 
GGGGCGGCAG CCGTCGGTGT CCGGGCTTGC AGCTCCACGG CCGCCCCGGA CGGCGTCGAG 
GGCCCGGCGC TGCGGCGCTC CTATTGGCGC CACCTGAGGC GTCTGGTGCT GGGTCCTCCC 
GAACCGCCGT TCTCGCACGT GTGCCAAGTC GGGGACCCGG TGCTGCGCGG CGTGGCGGCC 
CCGGTGGAGC GGGCGCAGCT AGGCGGGCCC GAGCTGCAGC GGCTGACGCA ACGGCTGGTC 
CAGGTGATGC GGCGGCGGCG CTGCGTGGGC CTAAGCGCGC CGCAGCTGGG GGTGCCGCGG 
CAGGTGCTGG CGCTGGAGCT CCCCGAGGCG CTGTGTCGGG AGTGCCCGCC CCGCCAGCGC 
GCGCTCCGCC AAATGGAGCC CTTCCCCCTG CGCGTGTTCG TGAACCCCAG CCTGCGAGTG 
CTTGACAGCC GCCTGGTCAC CTTTCCCGAG GGCTGCGAGA GCGTCGCCGG CTTCCTGGCC 
TGCGTGCCCC GCTTCCAGGC GGTGCAGATC TCAGGGCTGG ACCCCAATGG AGAACAGGTG 
GTGTGGCAGG CGAGCGGGTG GGCAGCCCGC ATCATCCAGC ACGAGATGGA CCACCTGCAG 
GGCTGCCTGT TTATTGACAA AATGGACAGC AGGACGTTCA CAAACGTCTA TTGGATGAAG 
GTGAATGACT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Structure-Based Drug Design of Small Molecule Peptide Deformylase Inhibitors to Treat Cancer.
Molecules (Basel, Switzerland)21(4)396(2016 Mar)
Gao J,Wang T,Qiu S,Zhu Y,Liang L,Zheng Y

Inhibition of human mitochondrial peptide deformylase causes apoptosis in c-myc-overexpressing hematopoietic cancers.
Cell death & disease5e1152(2014 Mar)
Sheth A,Escobar-Alvarez S,Gardner J,Ran L,Heaney ML,Scheinberg DA

Overexpression of peptide deformylase in breast, colon, and lung cancers.
BMC cancer13321(2013 Jul)
Randhawa H,Chikara S,Gehring D,Yildirim T,Menon J,Reindl KM

Transcriptional regulation of the human mitochondrial peptide deformylase (PDF).
Biochemical and biophysical research communications421(4)825-31(2012 May)
Pereira-Castro I,Costa LT,Amorim A,Azevedo L

Successful COG8 and PDF overlap is mediated by alterations in splicing and polyadenylation signals.
Human genetics131(2)265-74(2012 Feb)
Pereira-Castro I,Quental R,da Costa LT,Amorim A,Azevedo L

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PDF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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