GFRA4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	GFRA4
Entrez Gene ID	64096
Full Name	GDNF family receptor alpha 4
General protein information	Preferred Names GDNF family receptor alpha 4 Names GDNF family receptor alpha-4 GDNF receptor alpha-4 GDNFR-alpha-4 GFR receptor alpha 4 GFR-alpha-4 persephin receptor
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20p13
Summary	The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for persephin, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for RET-associated diseases. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008].

mRNA and Protein(s)

mRNA	Protein	Name
XM_005260793.2	XP_005260850.1	GDNF family receptor alpha-4 isoform X1
NM_145762.2	NP_665705.1	GDNF family receptor alpha-4 isoform b precursor
NM_022139.3	NP_071422.1	GDNF family receptor alpha-4 isoform a precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GFRA4	XP_851489.2
Rattus norvegicus (Norway rat)	Gfra4	NP_076457.1
Homo sapiens (human)	GFRA4	NP_071422.1
Mus musculus (house mouse)	Gfra4	NP_064398.1
Pan troglodytes (chimpanzee)	GFRA4	XP_001168964.2
	LOC718481	XP_001111233.1
Bos taurus (cattle)	GFRA4	XP_002692431.2

Gene Ontology
Bibliography

Related articles in PubMed

Downregulation of microRNA-483-5p Promotes Cell Proliferation and Invasion by Targeting GFRA4 in Hirschsprung's Disease.
Wang G, Guo F, Wang H, Liu W, Zhang L, Cui M, Wu X
DNA and cell biology36(11)930-937(2017 Nov)

PSPN/GFRalpha4 has a significantly weaker capacity than GDNF/GFRalpha1 to recruit RET to rafts, but promotes neuronal survival and neurite outgrowth.
Yang J, Lindahl M, Lindholm P, Virtanen H, Coffey E, Runeberg-Roos P, Saarma M
FEBS letters569(1-3)267-71(2004 Jul)

Investigation of germline GFRA4 mutations and evaluation of the involvement of GFRA1, GFRA2, GFRA3, and GFRA4 sequence variants in Hirschsprung disease.
Borrego S, Fern?ndez RM, Dziema H, Niess A, L?pez-Alonso M, Anti?olo G, Eng C
Journal of medical genetics40(3)e18(2003 Mar)

The DNA sequence and comparative analysis of human chromosome 20.
Deloukas P, Matthews LH, Ashurst J, Burton J, Gilbert JG, Jones M, Stavrides G, Almeida JP, Babbage AK, Bagguley CL, Bailey J, Barlow KF, Bates KN, Beard LM, Beare DM, Beasley OP, Bird CP, Blakey SE, Bridgeman AM, Brown AJ, Buck D, Burrill W, Butler AP, Carder C, Carter NP, Chapman JC, Clamp M, Clark G, Clark LN, Clark SY, Clee CM, Clegg S, Cobley VE, Collier RE, Connor R, Corby NR, Coulson A, Coville GJ, Deadman R, Dhami P, Dunn M, Ellington AG, Frankland JA, Fraser A, French L, Garner P, Grafham DV, Griffiths C, Griffiths MN, Gwilliam R, Hall RE, Hammond S, Harley JL, Heath PD, Ho S, Holden JL, Howden PJ, Huckle E, Hunt AR, Hunt SE, Jekosch K, Johnson CM, Johnson D, Kay MP, Kimberley AM, King A, Knights A, Laird GK, Lawlor S, Lehvaslaiho MH, Leversha M, Lloyd C, Lloyd DM, Lovell JD, Marsh VL, Martin SL, McConnachie LJ, McLay K, McMurray AA, Milne S, Mistry D, Moore MJ, Mullikin JC, Nickerson T, Oliver K, Parker A, Patel R, Pearce TA, Peck AI, Phillimore BJ, Prathalingam SR, Plumb RW, Ramsay H, Rice CM, Ross MT, Scott CE, Sehra HK, Shownkeen R, Sims S, Skuce CD, Smith ML, Soderlund C, Steward CA, Sulston JE, Swann M, Sycamore N, Taylor R, Tee L, Thomas DW, Thorpe A, Tracey A, Tromans AC, Vaudin M, Wall M, Wallis JM, Whitehead SL, Whittaker P, Willey DL, Williams L, Williams SA, Wilming L, Wray PW, Hubbard T, Durbin RM, Bentley DR, Beck S, Rogers J
Nature414(6866)865-71(2001 Dec 20-27)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

High GFRA4 expression is associated with Hirschsprung's Disease.
Title: Downregulation of microRNA-483-5p Promotes Cell Proliferation and Invasion by Targeting GFRA4 in Hirschsprung's Disease.

GDNF/GFRalpha1 is crucial for efficient signal transduction to recruit Ret protein into lipid rafts.
Title: PSPN/GFRalpha4 has a significantly weaker capacity than GDNF/GFRalpha1 to recruit RET to rafts, but promotes neuronal survival and neurite outgrowth.

Observational study of gene-disease association. (HuGE Navigator)
Title: Investigation of germline GFRA4 mutations and evaluation of the involvement of GFRA1, GFRA2, GFRA3, and GFRA4 sequence variants in Hirschsprung disease.

The following GFRA4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GFRA4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30403	NM_145762.2 Latest version!	Homo sapiens GDNF family receptor alpha 4 (GFRA4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$99.50-$139.30 $199.00
OHu30147	NM_022139.3 Latest version!	Homo sapiens GDNF family receptor alpha 4 (GFRA4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$139.30 $199.00
OHu52758	XM_005260793.2 Latest version!	Homo sapiens GDNF family receptor alpha 4 (GFRA4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GFRA4 ( NM_145762.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_665705.1 Homo sapiens GDNF family receptor alpha 4 (GFRA4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu30403
Clone ID Related Accession (Same CDS sequence)	NM_145762.2
Accession Version	NM_145762.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 900bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-25
Organism	Homo sapiens(human)
Product	GDNF family receptor alpha-4 isoform b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AJ291674.1 and AL356755.12. On May 19, 2006 this sequence version replaced NM_145762.1. Transcript Variant: This variant (2) includes additional segments in the coding region compared to variant 1, that cause internal frameshift. The resulting isoform (b) contains additional aa in the middle of the protein, but has the same N- and C-terminal ends as isoform a. ##Evidence-Data-START## Transcript exon combination :: AJ291674.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2144120, SAMEA2148874 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END##

ATGGTCCGCT GCCTGGGGCC TGCGCTGCTG CTGCTGCTGT TACTGGGGTC GGCGAGCTCG 
GTCGGAGGGA ACCGATGTGT GGACGCGGCC GAAGCCTGCA CGGCGGACGC GCGGTGCCAG 
CGTTTGCGCT CCGAGTATGT GGCGCAGTGC CTGGGCCGGG CTGCGCAGGG GGGCTGTCCC 
CGCGCCCGCT GCCGCCGGGC CCTGCGCCGC TTCTTCGCCC GCGGGCCGCC CGCGCTCACC 
CACGCACTGC TCTTCTGCCC GTGCGCGGGC CCCGCGTGCG CCGAGCGTCG GCGCCAGACC 
TTCGTGCCCT CCTGCGCCTT TTCGGGGCCC GGCCCCGCGC CGCCCTCCTG CCTTGAGCCC 
TTAAACTTCT GCGAGCGCAG CCGGGTCTGC AGGTGCGCGC GGGCGGCGGC GGGGCCGTGG 
CGAGGGTGGG GACGGGGCCT CTCTCCGGCT CACCGCCCTC CCGCCGCGCA GGCCTCGCCT 
CCTGGCCTTT CAGGTCTCGT GCACCCCAGC GCCCAGCGCC CCCGACGGCT GCCTGCTGGA 
CCAGGGCGCC CGCTGCCTGC GCGCCTACGC GGGCCTCGTG GGGTCCCCGC AGGCACCGCC 
GTCACCCCTA ACTACGTGGA CAACGTGAGC GCGCGCGTGG CGCCCTGGTG CGACTGCGGA 
GCCAGCGGGA ACCGGCGTGA GGACTGCGAA GCCTTCCGGG GGCTCTTTAC CAGGAACCGC 
TGCTTGGATG GTGCCATTCA GGCCTTTGCC AGCGGGTGGC CCCCAGTCCT GCTGGACCAG 
CTGAACCCCC AGGGAGACCC GGAGCACAGC CTCCTGCAGG TGTCCTCCAC AGGCAGGGCC 
CTGGAGAGAC GCTCCCTGCT CTCCATACTT CCTGTCCTGG CTCTCCCGGC CCTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_665705.1
CDS	1..900
Translation	MVRCLGPALLLLLLLGSASSVGGNRCVDAAEACTADARCQRLRSEYVAQCLGRAAQGGCPRARCRRALRRFFARGPPALTHALLFCPCAGPACAERRRQTFVPSCAFSGPGPAPPSCLEPLNFCERSRVCRCARAAAGPWRGWGRGLSPAHRPPAAQASPPGLSGLVHPSAQRPRRLPAGPGRPLPARLRGPRGVPAGTAVTPNYVDNVSARVAPWCDCGASGNRREDCEAFRGLFTRNRCLDGAIQAFASGWPPVLLDQLNPQGDPEHSLLQVSSTGRALERRSLLSILPVLALPALL

Target ORF information:

RefSeq Version	NM_145762.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GDNF family receptor alpha 4 (GFRA4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_145762.2

ORF Insert Sequence:

ATGGTCCGCT GCCTGGGGCC TGCGCTGCTG CTGCTGCTGT TACTGGGGTC GGCGAGCTCG 
GTCGGAGGGA ACCGATGTGT GGACGCGGCC GAAGCCTGCA CGGCGGACGC GCGGTGCCAG 
CGTTTGCGCT CCGAGTATGT GGCGCAGTGC CTGGGCCGGG CTGCGCAGGG GGGCTGTCCC 
CGCGCCCGCT GCCGCCGGGC CCTGCGCCGC TTCTTCGCCC GCGGGCCGCC CGCGCTCACC 
CACGCACTGC TCTTCTGCCC GTGCGCGGGC CCCGCGTGCG CCGAGCGTCG GCGCCAGACC 
TTCGTGCCCT CCTGCGCCTT TTCGGGGCCC GGCCCCGCGC CGCCCTCCTG CCTTGAGCCC 
TTAAACTTCT GCGAGCGCAG CCGGGTCTGC AGGTGCGCGC GGGCGGCGGC GGGGCCGTGG 
CGAGGGTGGG GACGGGGCCT CTCTCCGGCT CACCGCCCTC CCGCCGCGCA GGCCTCGCCT 
CCTGGCCTTT CAGGTCTCGT GCACCCCAGC GCCCAGCGCC CCCGACGGCT GCCTGCTGGA 
CCAGGGCGCC CGCTGCCTGC GCGCCTACGC GGGCCTCGTG GGGTCCCCGC AGGCACCGCC 
GTCACCCCTA ACTACGTGGA CAACGTGAGC GCGCGCGTGG CGCCCTGGTG CGACTGCGGA 
GCCAGCGGGA ACCGGCGTGA GGACTGCGAA GCCTTCCGGG GGCTCTTTAC CAGGAACCGC 
TGCTTGGATG GTGCCATTCA GGCCTTTGCC AGCGGGTGGC CCCCAGTCCT GCTGGACCAG 
CTGAACCCCC AGGGAGACCC GGAGCACAGC CTCCTGCAGG TGTCCTCCAC AGGCAGGGCC 
CTGGAGAGAC GCTCCCTGCT CTCCATACTT CCTGTCCTGG CTCTCCCGGC CCTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GFRA4 ( NM_022139.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_071422.1 Homo sapiens GDNF family receptor alpha 4 (GFRA4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu30147
Clone ID Related Accession (Same CDS sequence)	NM_022139.3
Accession Version	NM_022139.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 810bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-25
Organism	Homo sapiens(human)
Product	GDNF family receptor alpha-4 isoform a precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF253318.1 and AL356755.12. On May 19, 2006 this sequence version replaced NM_022139.2. Transcript Variant: This variant (1) encodes the predominant isoform (a). ##Evidence-Data-START## Transcript exon combination :: AF253318.1, AJ291673.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2144120, SAMEA2148874 [ECO:0000350] ##Evidence-Data-END##

ATGGTCCGCT GCCTGGGGCC TGCGCTGCTG CTGCTGCTGT TACTGGGGTC GGCGAGCTCG 
GTCGGAGGGA ACCGATGTGT GGACGCGGCC GAAGCCTGCA CGGCGGACGC GCGGTGCCAG 
CGTTTGCGCT CCGAGTATGT GGCGCAGTGC CTGGGCCGGG CTGCGCAGGG GGGCTGTCCC 
CGCGCCCGCT GCCGCCGGGC CCTGCGCCGC TTCTTCGCCC GCGGGCCGCC CGCGCTCACC 
CACGCACTGC TCTTCTGCCC GTGCGCGGGC CCCGCGTGCG CCGAGCGTCG GCGCCAGACC 
TTCGTGCCCT CCTGCGCCTT TTCGGGGCCC GGCCCCGCGC CGCCCTCCTG CCTTGAGCCC 
TTAAACTTCT GCGAGCGCAG CCGGGTCTGC AGGCCTCGCC TCCTGGCCTT TCAGGTCTCG 
TGCACCCCAG CGCCCAGCGC CCCCGACGGC TGCCTGCTGG ACCAGGGCGC CCGCTGCCTG 
CGCGCCTACG CGGGCCTCGT GGGCACCGCC GTCACCCCTA ACTACGTGGA CAACGTGAGC 
GCGCGCGTGG CGCCCTGGTG CGACTGCGGA GCCAGCGGGA ACCGGCGTGA GGACTGCGAA 
GCCTTCCGGG GGCTCTTTAC CAGGAACCGC TGCTTGGATG GTGCCATTCA GGCCTTTGCC 
AGCGGGTGGC CCCCAGTCCT GCTGGACCAG CTGAACCCCC AGGGAGACCC GGAGCACAGC 
CTCCTGCAGG TGTCCTCCAC AGGCAGGGCC CTGGAGAGAC GCTCCCTGCT CTCCATACTT 
CCTGTCCTGG CTCTCCCGGC CCTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071422.1
CDS	1..810
Translation	MVRCLGPALLLLLLLGSASSVGGNRCVDAAEACTADARCQRLRSEYVAQCLGRAAQGGCPRARCRRALRRFFARGPPALTHALLFCPCAGPACAERRRQTFVPSCAFSGPGPAPPSCLEPLNFCERSRVCRPRLLAFQVSCTPAPSAPDGCLLDQGARCLRAYAGLVGTAVTPNYVDNVSARVAPWCDCGASGNRREDCEAFRGLFTRNRCLDGAIQAFASGWPPVLLDQLNPQGDPEHSLLQVSSTGRALERRSLLSILPVLALPALL

Target ORF information:

RefSeq Version	NM_022139.3
Organism	Homo sapiens(human)
Definition	Homo sapiens GDNF family receptor alpha 4 (GFRA4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022139.3

ORF Insert Sequence:

ATGGTCCGCT GCCTGGGGCC TGCGCTGCTG CTGCTGCTGT TACTGGGGTC GGCGAGCTCG 
GTCGGAGGGA ACCGATGTGT GGACGCGGCC GAAGCCTGCA CGGCGGACGC GCGGTGCCAG 
CGTTTGCGCT CCGAGTATGT GGCGCAGTGC CTGGGCCGGG CTGCGCAGGG GGGCTGTCCC 
CGCGCCCGCT GCCGCCGGGC CCTGCGCCGC TTCTTCGCCC GCGGGCCGCC CGCGCTCACC 
CACGCACTGC TCTTCTGCCC GTGCGCGGGC CCCGCGTGCG CCGAGCGTCG GCGCCAGACC 
TTCGTGCCCT CCTGCGCCTT TTCGGGGCCC GGCCCCGCGC CGCCCTCCTG CCTTGAGCCC 
TTAAACTTCT GCGAGCGCAG CCGGGTCTGC AGGCCTCGCC TCCTGGCCTT TCAGGTCTCG 
TGCACCCCAG CGCCCAGCGC CCCCGACGGC TGCCTGCTGG ACCAGGGCGC CCGCTGCCTG 
CGCGCCTACG CGGGCCTCGT GGGCACCGCC GTCACCCCTA ACTACGTGGA CAACGTGAGC 
GCGCGCGTGG CGCCCTGGTG CGACTGCGGA GCCAGCGGGA ACCGGCGTGA GGACTGCGAA 
GCCTTCCGGG GGCTCTTTAC CAGGAACCGC TGCTTGGATG GTGCCATTCA GGCCTTTGCC 
AGCGGGTGGC CCCCAGTCCT GCTGGACCAG CTGAACCCCC AGGGAGACCC GGAGCACAGC 
CTCCTGCAGG TGTCCTCCAC AGGCAGGGCC CTGGAGAGAC GCTCCCTGCT CTCCATACTT 
CCTGTCCTGG CTCTCCCGGC CCTGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GFRA4 ( XM_005260793.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005260850.1 Homo sapiens GDNF family receptor alpha 4 (GFRA4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu52758
Clone ID Related Accession (Same CDS sequence)	XM_005260793.2
Accession Version	XM_005260793.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 549bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	GDNF family receptor alpha-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005260793.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCGCT GCCTGGGGCC TGCGCTGCTG CTGCTGCTGT TACTGGGGTC GGCGAGCTCG 
GTCGGAGGGA ACCGATGTGT GGACGCGGCC GAAGCCTGCA CGGCGGACGC GCGGTGCCAG 
CGTTTGCGCT CCGAGTATGT GGCGCAGTGC CTGGGCCGGG CTGCGCAGGG GGGCTGTCCC 
CGCGCCCGCT GCCGCCGGGC CCTGCGCCGC TTCTTCGCCC GCGGGCCGCC CGCGCTCACC 
CACGCACTGC TCTTCTGCCC GTGCGCGGGC CCCGCGTGCG CCGAGCGTCG GCGCCAGACC 
TTCGTGCCCT CCTGCGCCTT TTCGGGGCCC GGCCCCGCGC CGCCCTCCTG CCTTGAGCCC 
TTAAACTTCT GCGAGCGCAG CCGGGTCTGC AGGCCTCGCC TCCTGGCCTT TCAGGTCTCG 
TGCACCCCAG CGCCCAGCGC CCCCGACGGC TGCCTGCTGG ACCAGGGCGC CCGCTGCCTG 
CGCGCCTACG CGGGCCTCGT GGGGTCCCCG CAGGCACCGC CGTCACCCCT AACTACGTGG 
ACAACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005260850.1
CDS	1..549
Translation	MVRCLGPALLLLLLLGSASSVGGNRCVDAAEACTADARCQRLRSEYVAQCLGRAAQGGCPRARCRRALRRFFARGPPALTHALLFCPCAGPACAERRRQTFVPSCAFSGPGPAPPSCLEPLNFCERSRVCRPRLLAFQVSCTPAPSAPDGCLLDQGARCLRAYAGLVGSPQAPPSPLTTWTT

Target ORF information:

RefSeq Version	XM_005260793.2
Organism	Homo sapiens(human)
Definition	Homo sapiens GDNF family receptor alpha 4 (GFRA4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260793.2

ORF Insert Sequence:

ATGGTCCGCT GCCTGGGGCC TGCGCTGCTG CTGCTGCTGT TACTGGGGTC GGCGAGCTCG 
GTCGGAGGGA ACCGATGTGT GGACGCGGCC GAAGCCTGCA CGGCGGACGC GCGGTGCCAG 
CGTTTGCGCT CCGAGTATGT GGCGCAGTGC CTGGGCCGGG CTGCGCAGGG GGGCTGTCCC 
CGCGCCCGCT GCCGCCGGGC CCTGCGCCGC TTCTTCGCCC GCGGGCCGCC CGCGCTCACC 
CACGCACTGC TCTTCTGCCC GTGCGCGGGC CCCGCGTGCG CCGAGCGTCG GCGCCAGACC 
TTCGTGCCCT CCTGCGCCTT TTCGGGGCCC GGCCCCGCGC CGCCCTCCTG CCTTGAGCCC 
TTAAACTTCT GCGAGCGCAG CCGGGTCTGC AGGCCTCGCC TCCTGGCCTT TCAGGTCTCG 
TGCACCCCAG CGCCCAGCGC CCCCGACGGC TGCCTGCTGG ACCAGGGCGC CCGCTGCCTG 
CGCGCCTACG CGGGCCTCGT GGGGTCCCCG CAGGCACCGC CGTCACCCCT AACTACGTGG 
ACAACGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Downregulation of microRNA-483-5p Promotes Cell Proliferation and Invasion by Targeting GFRA4 in Hirschsprung's Disease.
DNA and cell biology36(11)930-937(2017 Nov)
Wang G,Guo F,Wang H,Liu W,Zhang L,Cui M,Wu X

PSPN/GFRalpha4 has a significantly weaker capacity than GDNF/GFRalpha1 to recruit RET to rafts, but promotes neuronal survival and neurite outgrowth.
FEBS letters569(1-3)267-71(2004 Jul)
Yang J,Lindahl M,Lindholm P,Virtanen H,Coffey E,Runeberg-Roos P,Saarma M

Investigation of germline GFRA4 mutations and evaluation of the involvement of GFRA1, GFRA2, GFRA3, and GFRA4 sequence variants in Hirschsprung disease.
Journal of medical genetics40(3)e18(2003 Mar)
Borrego S,Fern?ndez RM,Dziema H,Niess A,L?pez-Alonso M,Anti?olo G,Eng C

The DNA sequence and comparative analysis of human chromosome 20.
Nature414(6866)865-71(2001 Dec 20-27)
Deloukas P,Matthews LH,Ashurst J,Burton J,Gilbert JG,Jones M,Stavrides G,Almeida JP,Babbage AK,Bagguley CL,Bailey J,Barlow KF,Bates KN,Beard LM,Beare DM,Beasley OP,Bird CP,Blakey SE,Bridgeman AM,Brown AJ,Buck D,Burrill W,Butler AP,Carder C,Carter NP,Chapman JC,Clamp M,Clark G,Clark LN,Clark SY,Clee CM,Clegg S,Cobley VE,Collier RE,Connor R,Corby NR,Coulson A,Coville GJ,Deadman R,Dhami P,Dunn M,Ellington AG,Frankland JA,Fraser A,French L,Garner P,Grafham DV,Griffiths C,Griffiths MN,Gwilliam R,Hall RE,Hammond S,Harley JL,Heath PD,Ho S,Holden JL,Howden PJ,Huckle E,Hunt AR,Hunt SE,Jekosch K,Johnson CM,Johnson D,Kay MP,Kimberley AM,King A,Knights A,Laird GK,Lawlor S,Lehvaslaiho MH,Leversha M,Lloyd C,Lloyd DM,Lovell JD,Marsh VL,Martin SL,McConnachie LJ,McLay K,McMurray AA,Milne S,Mistry D,Moore MJ,Mullikin JC,Nickerson T,Oliver K,Parker A,Patel R,Pearce TA,Peck AI,Phillimore BJ,Prathalingam SR,Plumb RW,Ramsay H,Rice CM,Ross MT,Scott CE,Sehra HK,Shownkeen R,Sims S,Skuce CD,Smith ML,Soderlund C,Steward CA,Sulston JE,Swann M,Sycamore N,Taylor R,Tee L,Thomas DW,Thorpe A,Tracey A,Tromans AC,Vaudin M,Wall M,Wallis JM,Whitehead SL,Whittaker P,Willey DL,Williams L,Williams SA,Wilming L,Wray PW,Hubbard T,Durbin RM,Bentley DR,Beck S,Rogers J

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GFRA4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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