ANKEF1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ANKEF1
Entrez Gene ID	63926
Full Name	ankyrin repeat and EF-hand domain containing 1
Synonyms	ANKRD5
General protein information	Preferred Names ankyrin repeat and EF-hand domain containing 1 Names ankyrin repeat and EF-hand domain-containing protein 1 ankyrin repeat domain-containing protein 5
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20p12.2

mRNA and Protein(s)

mRNA	Protein	Name
NM_198798.2	NP_942093.1	ankyrin repeat and EF-hand domain-containing protein 1 isoform 1
NM_198798.3	NP_942093.1	ankyrin repeat and EF-hand domain-containing protein 1 isoform 1
XM_005260792.2	XP_005260849.1	ankyrin repeat and EF-hand domain-containing protein 1 isoform X1
NM_001303472.1	NP_001290401.1	ankyrin repeat and EF-hand domain-containing protein 1 isoform 2
NM_022096.6	NP_071379.3	ankyrin repeat and EF-hand domain-containing protein 1 isoform 1
NM_022096.5	NP_071379.3	ankyrin repeat and EF-hand domain-containing protein 1 isoform 1
NM_001303472.2	NP_001290401.1	ankyrin repeat and EF-hand domain-containing protein 1 isoform 2

Pathways from BioSystems

Homologies

Homo sapiens (human)	ANKEF1	NP_942093.1
	ANKRD5	XP_001116085.1
Canis lupus familiaris (dog)	ANKEF1	XP_534348.1
Bos taurus (cattle)	ANKEF1	XP_002692084.1
Pan troglodytes (chimpanzee)	ANKRD5	XP_514513.3
Mus musculus (house mouse)	Ankef1	NP_783598.1
Gallus gallus (chicken)	LOC416735	XP_004935298.1
Xenopus tropicalis (tropical clawed frog)	ankef1	NP_001072351.1
Rattus norvegicus (Norway rat)	Ankef1	NP_001099986.1
Danio rerio (zebrafish)	ankef1a	NP_001020714.1

Gene Ontology
Bibliography

Related articles in PubMed

Sex-specific linkage to total serum immunoglobulin E in families of children with asthma in Costa Rica.
Raby BA, Soto-Quiros ME, Avila L, Lake SL, Murphy A, Liang C, Fournier E, Spesny M, Sylvia JS, Verner A, Hudson TJ, Klanderman BJ, Freimer NB, Silverman EK, Celed?n JC
Human molecular genetics16(3)243-53(2007 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ANKEF1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKEF1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03802	NM_022096.6 Latest version!	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$279.50-$391.30 $559.00
OHu52757	NM_001303472.2 Latest version!	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu52757	XM_005260792.2 Latest version!	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OHu03802	NM_198798.3 Latest version!	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
View Old Accession Versions >>
OHu03802	NM_198798.2	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu03802	NM_022096.5	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$265.30 $379.00
OHu52757	NM_001303472.1	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ANKEF1 ( NM_022096.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_071379.3 Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03802
Clone ID Related Accession (Same CDS sequence)	NM_198798.2 , NM_198798.3 , NM_022096.6 , NM_022096.5
Accession Version	NM_022096.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-01
Organism	Homo sapiens(human)
Product	ankyrin repeat and EF-hand domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL109754.13, BP279314.1, BI562273.1, AK097689.1, AK025322.1 and BU621946.1. On Nov 22, 2018 this sequence version replaced NM_022096.5. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660809.84743.1, BC022878.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000378392.6/ ENSP00000367644.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCTTTAG CAGATAAGAG ACTTGAGAAC TTACAGATCT ACAAAGTTCT TCAATGTGTG 
CGGAACAAAG ACAAGAAGCA GATAGAGAAG CTGACCAAGC TTGGATACCC TGAACTAATC 
AATTATACAG AACCCATTAA TGGACTTAGT GCTTTGCACT TAGCCTCAGT TTCCAATGAT 
ATTGATATGG TCAGCTTTCT CCTTGACCTT GGTGCTCACC CTGATGTGCA AGACCGAATG 
GGCTGTACTC CCACAATGAG GGCTGCAGAA CTGGGCCATG AATTGTCAAT GGAAATATTA 
GCAAAGGCAA AGGCTGATAT GACTATAGTT GATAATGAAG GAAAAGGTGT TTTGTTTTAC 
TGCATTTTAC CGACTAAGCG GCATTATCGC TGTGCTCTGA TCGCCCTTGA ACATGGTGCA 
GATGTCAACA ATTCTACCTA TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGATGCA 
CATGATGTTA AAGATGTGTG CCTGACATTT TTGGAAAAAG GAGCCAATCC TAATGCAATC 
AACTCATCCA CAGGCCGCAC AGCTTTAATG GAAGCGTCAA GAGAAGGGGT AGTGGAAATA 
GTTCGAGGCA TATTGGAAAG AGGAGGTGAA GTGAATGCAT TTGACAACGA CAGGCATCAC 
GCTGCTCATT TTGCTGCTAA AGGAGGCTTT TTCGATATAT TGAAGCTTCT TTTTGCCTAC 
AATGGAGACG TGGGGCTGAT TTCGATAAAT GGGAACACAC CACTTCATTA TGCTGCCATG 
GGTGGTTTTG CAGACTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT GAAATGGAAG 
AATTTAGATC ATAAAACGCC CAGGGCTGTG GCTAAGGAAG GCGGCTTCAA AGCAGCAAGC 
AAAGAAATAC GCCGAGCAGA GAGAATCGCT AATAAACTAG CCAGGCCAGG AGCCAAAAAT 
CCAAATCCAC TGTGGGCCCT TAGACTGCAC GATTGGTCCG TAGAACGTGA GGCTTTCCTC 
CGGGAAGCCT TTGCGGTTTT AGACAGGGGT GATGGAAGCA TCAGCAAGAA CGACTTCGTG 
ATGGTGTTGG AGGAAAGGCA GGATTATGCA AGCTCAGAAC AGCTGGCTGC CATCGCTCAC 
CTTCATGAGA AAACCCGGGG AGGAGGGGTC AATATTAATG AATTCTTTAA AGGAACCAGA 
TATTTAAACA AGTCTTTTGT CTTAGGATCG TATGGACCTA AGAAAAAGGA AAAAGGGATG 
GGCAAAAAAG GAAAGAAAGG GAAATTTGTC TTACCCCTTC CAATCTGTGT CATTCCTGAG 
TACGCGTTTC CACGCCGGCA GGATGGTGGG CCACCGTATT ACATGATTGA GACCTACAAG 
AATGTCACTG ATAGCAGCCG GTTTAATAGA GATCATCCCC CAGAACATCC CATTCAGGAT 
GACTCTGTTT GGTACATTGA TGATTCAGAG AAGGTATTTT CAAACATTAA TATTATCACC 
AAAGCAGGGG ATCTGGCTTC TCTGAAAAAG GCCTTTGAAT CAGGAATACC TGTGGATATG 
AAGGATAATT ATTACAAAAC TCCGCTAATG ACGGCGTGTG CAAGTGGAAA CATAGATGTG 
GTCAAGTTTC TTCTTGAAAA AGGAGCTAAC GTTAATGCAA CAGATAACTT TCTGTGGACT 
CCACTTCATT TTGCATGCCA TGCAGGCCAA CAAGACATTG TTGAGCTTCT TGTTGAATCT 
GGAGCTTTAA TAGATGCAGC TTCAATCAAC AACTCAACTC CTTTAAATAG AGCCATTGAA 
AGCTGCAGAC TGGATACAGT AAAATACCTA CTTGATATTG GTGCTAAATT CCAGCTGGAA 
AATAGAAAAG GGCATAGTGC CATGGACGTT GCAAAGGCAT ATGCTGATTA TAGAATAATT 
GATCTGATTA AAGAAAAGCT AGATAACTTG CCGAAACCAG CAGAAAATCA AAAACTAAAA 
GGCAAGACAC CTCCTATACT GAAGACTGAA GGCCCTGAAA TTAAGAAAGA AGAGGAACTG 
CTGTCATCAA TTTATGGTGT ACCAACCACA TCAGAGGGAA AGAAAGTACA GAAGGGTAAT 
GTGGTTCATC TGAATTCATT GATTACCAGT GGTTATACTA AGAAAGTGGA TATCACATTT 
ATTCCACGGA GGATTTGGAG TCCTGAAGCC ACAACAGCAG AGCTGATCAG GAAGAGGGAA 
CTACGGCGAG AGAGGTTTAC ACATGAGGTG GACTTCGACG ATTTTATGAT GCCTTTTCAG 
AAGAACATCA CAGAGAAAGC TCGAGCACTG GAAGCTGCCT TGAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071379.3
CDS	456..2786
Translation	MALADKRLENLQIYKVLQCVRNKDKKQIEKLTKLGYPELINYTEPINGLSALHLASVSNDIDMVSFLLDLGAHPDVQDRMGCTPTMRAAELGHELSMEILAKAKADMTIVDNEGKGVLFYCILPTKRHYRCALIALEHGADVNNSTYEGKPIFLRACEDAHDVKDVCLTFLEKGANPNAINSSTGRTALMEASREGVVEIVRGILERGGEVNAFDNDRHHAAHFAAKGGFFDILKLLFAYNGDVGLISINGNTPLHYAAMGGFADCCKYIAQRGCDLKWKNLDHKTPRAVAKEGGFKAASKEIRRAERIANKLARPGAKNPNPLWALRLHDWSVEREAFLREAFAVLDRGDGSISKNDFVMVLEERQDYASSEQLAAIAHLHEKTRGGGVNINEFFKGTRYLNKSFVLGSYGPKKKEKGMGKKGKKGKFVLPLPICVIPEYAFPRRQDGGPPYYMIETYKNVTDSSRFNRDHPPEHPIQDDSVWYIDDSEKVFSNINIITKAGDLASLKKAFESGIPVDMKDNYYKTPLMTACASGNIDVVKFLLEKGANVNATDNFLWTPLHFACHAGQQDIVELLVESGALIDAASINNSTPLNRAIESCRLDTVKYLLDIGAKFQLENRKGHSAMDVAKAYADYRIIDLIKEKLDNLPKPAENQKLKGKTPPILKTEGPEIKKEEELLSSIYGVPTTSEGKKVQKGNVVHLNSLITSGYTKKVDITFIPRRIWSPEATTAELIRKRELRRERFTHEVDFDDFMMPFQKNITEKARALEAALKT

Target ORF information:

RefSeq Version	NM_022096.6
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022096.6

ORF Insert Sequence:

ATGGCTTTAG CAGATAAGAG ACTTGAGAAC TTACAGATCT ACAAAGTTCT TCAATGTGTG 
CGGAACAAAG ACAAGAAGCA GATAGAGAAG CTGACCAAGC TTGGATACCC TGAACTAATC 
AATTATACAG AACCCATTAA TGGACTTAGT GCTTTGCACT TAGCCTCAGT TTCCAATGAT 
ATTGATATGG TCAGCTTTCT CCTTGACCTT GGTGCTCACC CTGATGTGCA AGACCGAATG 
GGCTGTACTC CCACAATGAG GGCTGCAGAA CTGGGCCATG AATTGTCAAT GGAAATATTA 
GCAAAGGCAA AGGCTGATAT GACTATAGTT GATAATGAAG GAAAAGGTGT TTTGTTTTAC 
TGCATTTTAC CGACTAAGCG GCATTATCGC TGTGCTCTGA TCGCCCTTGA ACATGGTGCA 
GATGTCAACA ATTCTACCTA TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGATGCA 
CATGATGTTA AAGATGTGTG CCTGACATTT TTGGAAAAAG GAGCCAATCC TAATGCAATC 
AACTCATCCA CAGGCCGCAC AGCTTTAATG GAAGCGTCAA GAGAAGGGGT AGTGGAAATA 
GTTCGAGGCA TATTGGAAAG AGGAGGTGAA GTGAATGCAT TTGACAACGA CAGGCATCAC 
GCTGCTCATT TTGCTGCTAA AGGAGGCTTT TTCGATATAT TGAAGCTTCT TTTTGCCTAC 
AATGGAGACG TGGGGCTGAT TTCGATAAAT GGGAACACAC CACTTCATTA TGCTGCCATG 
GGTGGTTTTG CAGACTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT GAAATGGAAG 
AATTTAGATC ATAAAACGCC CAGGGCTGTG GCTAAGGAAG GCGGCTTCAA AGCAGCAAGC 
AAAGAAATAC GCCGAGCAGA GAGAATCGCT AATAAACTAG CCAGGCCAGG AGCCAAAAAT 
CCAAATCCAC TGTGGGCCCT TAGACTGCAC GATTGGTCCG TAGAACGTGA GGCTTTCCTC 
CGGGAAGCCT TTGCGGTTTT AGACAGGGGT GATGGAAGCA TCAGCAAGAA CGACTTCGTG 
ATGGTGTTGG AGGAAAGGCA GGATTATGCA AGCTCAGAAC AGCTGGCTGC CATCGCTCAC 
CTTCATGAGA AAACCCGGGG AGGAGGGGTC AATATTAATG AATTCTTTAA AGGAACCAGA 
TATTTAAACA AGTCTTTTGT CTTAGGATCG TATGGACCTA AGAAAAAGGA AAAAGGGATG 
GGCAAAAAAG GAAAGAAAGG GAAATTTGTC TTACCCCTTC CAATCTGTGT CATTCCTGAG 
TACGCGTTTC CACGCCGGCA GGATGGTGGG CCACCGTATT ACATGATTGA GACCTACAAG 
AATGTCACTG ATAGCAGCCG GTTTAATAGA GATCATCCCC CAGAACATCC CATTCAGGAT 
GACTCTGTTT GGTACATTGA TGATTCAGAG AAGGTATTTT CAAACATTAA TATTATCACC 
AAAGCAGGGG ATCTGGCTTC TCTGAAAAAG GCCTTTGAAT CAGGAATACC TGTGGATATG 
AAGGATAATT ATTACAAAAC TCCGCTAATG ACGGCGTGTG CAAGTGGAAA CATAGATGTG 
GTCAAGTTTC TTCTTGAAAA AGGAGCTAAC GTTAATGCAA CAGATAACTT TCTGTGGACT 
CCACTTCATT TTGCATGCCA TGCAGGCCAA CAAGACATTG TTGAGCTTCT TGTTGAATCT 
GGAGCTTTAA TAGATGCAGC TTCAATCAAC AACTCAACTC CTTTAAATAG AGCCATTGAA 
AGCTGCAGAC TGGATACAGT AAAATACCTA CTTGATATTG GTGCTAAATT CCAGCTGGAA 
AATAGAAAAG GGCATAGTGC CATGGACGTT GCAAAGGCAT ATGCTGATTA TAGAATAATT 
GATCTGATTA AAGAAAAGCT AGATAACTTG CCGAAACCAG CAGAAAATCA AAAACTAAAA 
GGCAAGACAC CTCCTATACT GAAGACTGAA GGCCCTGAAA TTAAGAAAGA AGAGGAACTG 
CTGTCATCAA TTTATGGTGT ACCAACCACA TCAGAGGGAA AGAAAGTACA GAAGGGTAAT 
GTGGTTCATC TGAATTCATT GATTACCAGT GGTTATACTA AGAAAGTGGA TATCACATTT 
ATTCCACGGA GGATTTGGAG TCCTGAAGCC ACAACAGCAG AGCTGATCAG GAAGAGGGAA 
CTACGGCGAG AGAGGTTTAC ACATGAGGTG GACTTCGACG ATTTTATGAT GCCTTTTCAG 
AAGAACATCA CAGAGAAAGC TCGAGCACTG GAAGCTGCCT TGAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKEF1 ( NM_001303472.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001290401.1 Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu52757
Clone ID Related Accession (Same CDS sequence)	XM_005260792.2 , NM_001303472.1 , NM_001303472.2
Accession Version	NM_001303472.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	ankyrin repeat and EF-hand domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP279314.1, BC028189.1, AK097689.1, AK025322.1, AL109754.13 and BU621946.1. Transcript Variant: This variant (3) contains multiple differences in the 5' region and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC028189.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCGT CAAGAGAAGG GGTAGTGGAA ATAGTTCGAG GCATATTGGA AAGAGGAGGT 
GAAGTGAATG CATTTGACAA CGACAGGCAT CACGCTGCTC ATTTTGCTGC TAAAGGAGGC 
TTTTTCGATA TATTGAAGCT TCTTTTTGCC TACAATGGAG ACGTGGGGCT GATTTCGATA 
AATGGGAACA CACCACTTCA TTATGCTGCC ATGGGTGGTT TTGCAGACTG CTGTAAATAT 
ATAGCTCAGC GAGGATGTGA CCTGAAATGG AAGAATTTAG ATCATAAAAC GCCCAGGGCT 
GTGGCTAAGG AAGGCGGCTT CAAAGCAGCA AGCAAAGAAA TACGCCGAGC AGAGAGAATC 
GCTAATAAAC TAGCCAGGCC AGGAGCCAAA AATCCAAATC CACTGTGGGC CCTTAGACTG 
CACGATTGGT CCGTAGAACG TGAGGCTTTC CTCCGGGAAG CCTTTGCGGT TTTAGACAGG 
GGTGATGGAA GCATCAGCAA GAACGACTTC GTGATGGTGT TGGAGGAAAG GCAGGATTAT 
GCAAGCTCAG AACAGCTGGC TGCCATCGCT CACCTTCATG AGAAAACCCG GGGAGGAGGG 
GTCAATATTA ATGAATTCTT TAAAGGAACC AGATATTTAA ACAAGTCTTT TGTCTTAGGA 
TCGTATGGAC CTAAGAAAAA GGAAAAAGGG ATGGGCAAAA AAGGAAAGAA AGGGAAATTT 
GTCTTACCCC TTCCAATCTG TGTCATTCCT GAGTACGCGT TTCCACGCCG GCAGGATGGT 
GGGCCACCGT ATTACATGAT TGAGACCTAC AAGAATGTCA CTGATAGCAG CCGGTTTAAT 
AGAGATCATC CCCCAGAACA TCCCATTCAG GATGACTCTG TTTGGTACAT TGATGATTCA 
GAGAAGGTAT TTTCAAACAT TAATATTATC ACCAAAGCAG GGGATCTGGC TTCTCTGAAA 
AAGGCCTTTG AATCAGGAAT ACCTGTGGAT ATGAAGGATA ATTATTACAA AACTCCGCTA 
ATGACGGCGT GTGCAAGTGG AAACATAGAT GTGGTCAAGT TTCTTCTTGA AAAAGGAGCT 
AACGTTAATG CAACAGATAA CTTTCTGTGG ACTCCACTTC ATTTTGCATG CCATGCAGGC 
CAACAAGACA TTGTTGAGCT TCTTGTTGAA TCTGGAGCTT TAATAGATGC AGCTTCAATC 
AACAACTCAA CTCCTTTAAA TAGAGCCATT GAAAGCTGCA GACTGGATAC AGTAAAATAC 
CTACTTGATA TTGGTGCTAA ATTCCAGCTG GAAAATAGAA AAGGGCATAG TGCCATGGAC 
GTTGCAAAGG CATATGCTGA TTATAGAATA ATTGATCTGA TTAAAGAAAA GCTAGATAAC 
TTGCCGAAAC CAGCAGAAAA TCAAAAACTA AAAGGCAAGA CACCTCCTAT ACTGAAGACT 
GAAGGCCCTG AAATTAAGAA AGAAGAGGAA CTGCTGTCAT CAATTTATGG TGTACCAACC 
ACATCAGAGG GAAAGAAAGT ACAGAAGGGT AATGTGGTTC ATCTGAATTC ATTGATTACC 
AGTGGTTATA CTAAGAAAGT GGATATCACA TTTATTCCAC GGAGGATTTG GAGTCCTGAA 
GCCACAACAG CAGAGCTGAT CAGGAAGAGG GAACTACGGC GAGAGAGGTT TACACATGAG 
GTGGACTTCG ACGATTTTAT GATGCCTTTT CAGAAGAACA TCACAGAGAA AGCTCGAGCA 
CTGGAAGCTG CCTTGAAGAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290401.1
CDS	993..2756
Translation	MEASREGVVEIVRGILERGGEVNAFDNDRHHAAHFAAKGGFFDILKLLFAYNGDVGLISINGNTPLHYAAMGGFADCCKYIAQRGCDLKWKNLDHKTPRAVAKEGGFKAASKEIRRAERIANKLARPGAKNPNPLWALRLHDWSVEREAFLREAFAVLDRGDGSISKNDFVMVLEERQDYASSEQLAAIAHLHEKTRGGGVNINEFFKGTRYLNKSFVLGSYGPKKKEKGMGKKGKKGKFVLPLPICVIPEYAFPRRQDGGPPYYMIETYKNVTDSSRFNRDHPPEHPIQDDSVWYIDDSEKVFSNINIITKAGDLASLKKAFESGIPVDMKDNYYKTPLMTACASGNIDVVKFLLEKGANVNATDNFLWTPLHFACHAGQQDIVELLVESGALIDAASINNSTPLNRAIESCRLDTVKYLLDIGAKFQLENRKGHSAMDVAKAYADYRIIDLIKEKLDNLPKPAENQKLKGKTPPILKTEGPEIKKEEELLSSIYGVPTTSEGKKVQKGNVVHLNSLITSGYTKKVDITFIPRRIWSPEATTAELIRKRELRRERFTHEVDFDDFMMPFQKNITEKARALEAALKT

Target ORF information:

RefSeq Version	NM_001303472.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303472.1

ORF Insert Sequence:

ATGGAAGCGT CAAGAGAAGG GGTAGTGGAA ATAGTTCGAG GCATATTGGA AAGAGGAGGT 
GAAGTGAATG CATTTGACAA CGACAGGCAT CACGCTGCTC ATTTTGCTGC TAAAGGAGGC 
TTTTTCGATA TATTGAAGCT TCTTTTTGCC TACAATGGAG ACGTGGGGCT GATTTCGATA 
AATGGGAACA CACCACTTCA TTATGCTGCC ATGGGTGGTT TTGCAGACTG CTGTAAATAT 
ATAGCTCAGC GAGGATGTGA CCTGAAATGG AAGAATTTAG ATCATAAAAC GCCCAGGGCT 
GTGGCTAAGG AAGGCGGCTT CAAAGCAGCA AGCAAAGAAA TACGCCGAGC AGAGAGAATC 
GCTAATAAAC TAGCCAGGCC AGGAGCCAAA AATCCAAATC CACTGTGGGC CCTTAGACTG 
CACGATTGGT CCGTAGAACG TGAGGCTTTC CTCCGGGAAG CCTTTGCGGT TTTAGACAGG 
GGTGATGGAA GCATCAGCAA GAACGACTTC GTGATGGTGT TGGAGGAAAG GCAGGATTAT 
GCAAGCTCAG AACAGCTGGC TGCCATCGCT CACCTTCATG AGAAAACCCG GGGAGGAGGG 
GTCAATATTA ATGAATTCTT TAAAGGAACC AGATATTTAA ACAAGTCTTT TGTCTTAGGA 
TCGTATGGAC CTAAGAAAAA GGAAAAAGGG ATGGGCAAAA AAGGAAAGAA AGGGAAATTT 
GTCTTACCCC TTCCAATCTG TGTCATTCCT GAGTACGCGT TTCCACGCCG GCAGGATGGT 
GGGCCACCGT ATTACATGAT TGAGACCTAC AAGAATGTCA CTGATAGCAG CCGGTTTAAT 
AGAGATCATC CCCCAGAACA TCCCATTCAG GATGACTCTG TTTGGTACAT TGATGATTCA 
GAGAAGGTAT TTTCAAACAT TAATATTATC ACCAAAGCAG GGGATCTGGC TTCTCTGAAA 
AAGGCCTTTG AATCAGGAAT ACCTGTGGAT ATGAAGGATA ATTATTACAA AACTCCGCTA 
ATGACGGCGT GTGCAAGTGG AAACATAGAT GTGGTCAAGT TTCTTCTTGA AAAAGGAGCT 
AACGTTAATG CAACAGATAA CTTTCTGTGG ACTCCACTTC ATTTTGCATG CCATGCAGGC 
CAACAAGACA TTGTTGAGCT TCTTGTTGAA TCTGGAGCTT TAATAGATGC AGCTTCAATC 
AACAACTCAA CTCCTTTAAA TAGAGCCATT GAAAGCTGCA GACTGGATAC AGTAAAATAC 
CTACTTGATA TTGGTGCTAA ATTCCAGCTG GAAAATAGAA AAGGGCATAG TGCCATGGAC 
GTTGCAAAGG CATATGCTGA TTATAGAATA ATTGATCTGA TTAAAGAAAA GCTAGATAAC 
TTGCCGAAAC CAGCAGAAAA TCAAAAACTA AAAGGCAAGA CACCTCCTAT ACTGAAGACT 
GAAGGCCCTG AAATTAAGAA AGAAGAGGAA CTGCTGTCAT CAATTTATGG TGTACCAACC 
ACATCAGAGG GAAAGAAAGT ACAGAAGGGT AATGTGGTTC ATCTGAATTC ATTGATTACC 
AGTGGTTATA CTAAGAAAGT GGATATCACA TTTATTCCAC GGAGGATTTG GAGTCCTGAA 
GCCACAACAG CAGAGCTGAT CAGGAAGAGG GAACTACGGC GAGAGAGGTT TACACATGAG 
GTGGACTTCG ACGATTTTAT GATGCCTTTT CAGAAGAACA TCACAGAGAA AGCTCGAGCA 
CTGGAAGCTG CCTTGAAGAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKEF1 ( NM_001303472.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001290401.1 Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 3, mRNA.

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CloneID	OHu52757
Clone ID Related Accession (Same CDS sequence)	XM_005260792.2 , NM_001303472.1 , NM_001303472.2
Accession Version	NM_001303472.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	ankyrin repeat and EF-hand domain-containing protein 1 isoform 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL109754.13, BP279314.1, BC028189.1, AK097689.1, AK025322.1 and BU621946.1. On May 31, 2019 this sequence version replaced NM_001303472.1. Transcript Variant: This variant (3) contains multiple differences in the 5' region and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus than isoform 1. ##Evidence-Data-START## Transcript exon combination :: BC028189.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145240 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGCGT CAAGAGAAGG GGTAGTGGAA ATAGTTCGAG GCATATTGGA AAGAGGAGGT 
GAAGTGAATG CATTTGACAA CGACAGGCAT CACGCTGCTC ATTTTGCTGC TAAAGGAGGC 
TTTTTCGATA TATTGAAGCT TCTTTTTGCC TACAATGGAG ACGTGGGGCT GATTTCGATA 
AATGGGAACA CACCACTTCA TTATGCTGCC ATGGGTGGTT TTGCAGACTG CTGTAAATAT 
ATAGCTCAGC GAGGATGTGA CCTGAAATGG AAGAATTTAG ATCATAAAAC GCCCAGGGCT 
GTGGCTAAGG AAGGCGGCTT CAAAGCAGCA AGCAAAGAAA TACGCCGAGC AGAGAGAATC 
GCTAATAAAC TAGCCAGGCC AGGAGCCAAA AATCCAAATC CACTGTGGGC CCTTAGACTG 
CACGATTGGT CCGTAGAACG TGAGGCTTTC CTCCGGGAAG CCTTTGCGGT TTTAGACAGG 
GGTGATGGAA GCATCAGCAA GAACGACTTC GTGATGGTGT TGGAGGAAAG GCAGGATTAT 
GCAAGCTCAG AACAGCTGGC TGCCATCGCT CACCTTCATG AGAAAACCCG GGGAGGAGGG 
GTCAATATTA ATGAATTCTT TAAAGGAACC AGATATTTAA ACAAGTCTTT TGTCTTAGGA 
TCGTATGGAC CTAAGAAAAA GGAAAAAGGG ATGGGCAAAA AAGGAAAGAA AGGGAAATTT 
GTCTTACCCC TTCCAATCTG TGTCATTCCT GAGTACGCGT TTCCACGCCG GCAGGATGGT 
GGGCCACCGT ATTACATGAT TGAGACCTAC AAGAATGTCA CTGATAGCAG CCGGTTTAAT 
AGAGATCATC CCCCAGAACA TCCCATTCAG GATGACTCTG TTTGGTACAT TGATGATTCA 
GAGAAGGTAT TTTCAAACAT TAATATTATC ACCAAAGCAG GGGATCTGGC TTCTCTGAAA 
AAGGCCTTTG AATCAGGAAT ACCTGTGGAT ATGAAGGATA ATTATTACAA AACTCCGCTA 
ATGACGGCGT GTGCAAGTGG AAACATAGAT GTGGTCAAGT TTCTTCTTGA AAAAGGAGCT 
AACGTTAATG CAACAGATAA CTTTCTGTGG ACTCCACTTC ATTTTGCATG CCATGCAGGC 
CAACAAGACA TTGTTGAGCT TCTTGTTGAA TCTGGAGCTT TAATAGATGC AGCTTCAATC 
AACAACTCAA CTCCTTTAAA TAGAGCCATT GAAAGCTGCA GACTGGATAC AGTAAAATAC 
CTACTTGATA TTGGTGCTAA ATTCCAGCTG GAAAATAGAA AAGGGCATAG TGCCATGGAC 
GTTGCAAAGG CATATGCTGA TTATAGAATA ATTGATCTGA TTAAAGAAAA GCTAGATAAC 
TTGCCGAAAC CAGCAGAAAA TCAAAAACTA AAAGGCAAGA CACCTCCTAT ACTGAAGACT 
GAAGGCCCTG AAATTAAGAA AGAAGAGGAA CTGCTGTCAT CAATTTATGG TGTACCAACC 
ACATCAGAGG GAAAGAAAGT ACAGAAGGGT AATGTGGTTC ATCTGAATTC ATTGATTACC 
AGTGGTTATA CTAAGAAAGT GGATATCACA TTTATTCCAC GGAGGATTTG GAGTCCTGAA 
GCCACAACAG CAGAGCTGAT CAGGAAGAGG GAACTACGGC GAGAGAGGTT TACACATGAG 
GTGGACTTCG ACGATTTTAT GATGCCTTTT CAGAAGAACA TCACAGAGAA AGCTCGAGCA 
CTGGAAGCTG CCTTGAAGAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001290401.1
CDS	1005..2768
Translation	MEASREGVVEIVRGILERGGEVNAFDNDRHHAAHFAAKGGFFDILKLLFAYNGDVGLISINGNTPLHYAAMGGFADCCKYIAQRGCDLKWKNLDHKTPRAVAKEGGFKAASKEIRRAERIANKLARPGAKNPNPLWALRLHDWSVEREAFLREAFAVLDRGDGSISKNDFVMVLEERQDYASSEQLAAIAHLHEKTRGGGVNINEFFKGTRYLNKSFVLGSYGPKKKEKGMGKKGKKGKFVLPLPICVIPEYAFPRRQDGGPPYYMIETYKNVTDSSRFNRDHPPEHPIQDDSVWYIDDSEKVFSNINIITKAGDLASLKKAFESGIPVDMKDNYYKTPLMTACASGNIDVVKFLLEKGANVNATDNFLWTPLHFACHAGQQDIVELLVESGALIDAASINNSTPLNRAIESCRLDTVKYLLDIGAKFQLENRKGHSAMDVAKAYADYRIIDLIKEKLDNLPKPAENQKLKGKTPPILKTEGPEIKKEEELLSSIYGVPTTSEGKKVQKGNVVHLNSLITSGYTKKVDITFIPRRIWSPEATTAELIRKRELRRERFTHEVDFDDFMMPFQKNITEKARALEAALKT

Target ORF information:

RefSeq Version	NM_001303472.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001303472.2

ORF Insert Sequence:

ATGGAAGCGT CAAGAGAAGG GGTAGTGGAA ATAGTTCGAG GCATATTGGA AAGAGGAGGT 
GAAGTGAATG CATTTGACAA CGACAGGCAT CACGCTGCTC ATTTTGCTGC TAAAGGAGGC 
TTTTTCGATA TATTGAAGCT TCTTTTTGCC TACAATGGAG ACGTGGGGCT GATTTCGATA 
AATGGGAACA CACCACTTCA TTATGCTGCC ATGGGTGGTT TTGCAGACTG CTGTAAATAT 
ATAGCTCAGC GAGGATGTGA CCTGAAATGG AAGAATTTAG ATCATAAAAC GCCCAGGGCT 
GTGGCTAAGG AAGGCGGCTT CAAAGCAGCA AGCAAAGAAA TACGCCGAGC AGAGAGAATC 
GCTAATAAAC TAGCCAGGCC AGGAGCCAAA AATCCAAATC CACTGTGGGC CCTTAGACTG 
CACGATTGGT CCGTAGAACG TGAGGCTTTC CTCCGGGAAG CCTTTGCGGT TTTAGACAGG 
GGTGATGGAA GCATCAGCAA GAACGACTTC GTGATGGTGT TGGAGGAAAG GCAGGATTAT 
GCAAGCTCAG AACAGCTGGC TGCCATCGCT CACCTTCATG AGAAAACCCG GGGAGGAGGG 
GTCAATATTA ATGAATTCTT TAAAGGAACC AGATATTTAA ACAAGTCTTT TGTCTTAGGA 
TCGTATGGAC CTAAGAAAAA GGAAAAAGGG ATGGGCAAAA AAGGAAAGAA AGGGAAATTT 
GTCTTACCCC TTCCAATCTG TGTCATTCCT GAGTACGCGT TTCCACGCCG GCAGGATGGT 
GGGCCACCGT ATTACATGAT TGAGACCTAC AAGAATGTCA CTGATAGCAG CCGGTTTAAT 
AGAGATCATC CCCCAGAACA TCCCATTCAG GATGACTCTG TTTGGTACAT TGATGATTCA 
GAGAAGGTAT TTTCAAACAT TAATATTATC ACCAAAGCAG GGGATCTGGC TTCTCTGAAA 
AAGGCCTTTG AATCAGGAAT ACCTGTGGAT ATGAAGGATA ATTATTACAA AACTCCGCTA 
ATGACGGCGT GTGCAAGTGG AAACATAGAT GTGGTCAAGT TTCTTCTTGA AAAAGGAGCT 
AACGTTAATG CAACAGATAA CTTTCTGTGG ACTCCACTTC ATTTTGCATG CCATGCAGGC 
CAACAAGACA TTGTTGAGCT TCTTGTTGAA TCTGGAGCTT TAATAGATGC AGCTTCAATC 
AACAACTCAA CTCCTTTAAA TAGAGCCATT GAAAGCTGCA GACTGGATAC AGTAAAATAC 
CTACTTGATA TTGGTGCTAA ATTCCAGCTG GAAAATAGAA AAGGGCATAG TGCCATGGAC 
GTTGCAAAGG CATATGCTGA TTATAGAATA ATTGATCTGA TTAAAGAAAA GCTAGATAAC 
TTGCCGAAAC CAGCAGAAAA TCAAAAACTA AAAGGCAAGA CACCTCCTAT ACTGAAGACT 
GAAGGCCCTG AAATTAAGAA AGAAGAGGAA CTGCTGTCAT CAATTTATGG TGTACCAACC 
ACATCAGAGG GAAAGAAAGT ACAGAAGGGT AATGTGGTTC ATCTGAATTC ATTGATTACC 
AGTGGTTATA CTAAGAAAGT GGATATCACA TTTATTCCAC GGAGGATTTG GAGTCCTGAA 
GCCACAACAG CAGAGCTGAT CAGGAAGAGG GAACTACGGC GAGAGAGGTT TACACATGAG 
GTGGACTTCG ACGATTTTAT GATGCCTTTT CAGAAGAACA TCACAGAGAA AGCTCGAGCA 
CTGGAAGCTG CCTTGAAGAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKEF1 ( XM_005260792.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_005260849.1 Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X1, mRNA.

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CloneID	OHu52757
Clone ID Related Accession (Same CDS sequence)	XM_005260792.2 , NM_001303472.1 , NM_001303472.2
Accession Version	XM_005260792.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1764bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	ankyrin repeat and EF-hand domain-containing protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 12, 2015 this sequence version replaced XM_005260792.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGCGT CAAGAGAAGG GGTAGTGGAA ATAGTTCGAG GCATATTGGA AAGAGGAGGT 
GAAGTGAATG CATTTGACAA CGACAGGCAT CACGCTGCTC ATTTTGCTGC TAAAGGAGGC 
TTTTTCGATA TATTGAAGCT TCTTTTTGCC TACAATGGAG ACGTGGGGCT GATTTCGATA 
AATGGGAACA CACCACTTCA TTATGCTGCC ATGGGTGGTT TTGCAGACTG CTGTAAATAT 
ATAGCTCAGC GAGGATGTGA CCTGAAATGG AAGAATTTAG ATCATAAAAC GCCCAGGGCT 
GTGGCTAAGG AAGGCGGCTT CAAAGCAGCA AGCAAAGAAA TACGCCGAGC AGAGAGAATC 
GCTAATAAAC TAGCCAGGCC AGGAGCCAAA AATCCAAATC CACTGTGGGC CCTTAGACTG 
CACGATTGGT CCGTAGAACG TGAGGCTTTC CTCCGGGAAG CCTTTGCGGT TTTAGACAGG 
GGTGATGGAA GCATCAGCAA GAACGACTTC GTGATGGTGT TGGAGGAAAG GCAGGATTAT 
GCAAGCTCAG AACAGCTGGC TGCCATCGCT CACCTTCATG AGAAAACCCG GGGAGGAGGG 
GTCAATATTA ATGAATTCTT TAAAGGAACC AGATATTTAA ACAAGTCTTT TGTCTTAGGA 
TCGTATGGAC CTAAGAAAAA GGAAAAAGGG ATGGGCAAAA AAGGAAAGAA AGGGAAATTT 
GTCTTACCCC TTCCAATCTG TGTCATTCCT GAGTACGCGT TTCCACGCCG GCAGGATGGT 
GGGCCACCGT ATTACATGAT TGAGACCTAC AAGAATGTCA CTGATAGCAG CCGGTTTAAT 
AGAGATCATC CCCCAGAACA TCCCATTCAG GATGACTCTG TTTGGTACAT TGATGATTCA 
GAGAAGGTAT TTTCAAACAT TAATATTATC ACCAAAGCAG GGGATCTGGC TTCTCTGAAA 
AAGGCCTTTG AATCAGGAAT ACCTGTGGAT ATGAAGGATA ATTATTACAA AACTCCGCTA 
ATGACGGCGT GTGCAAGTGG AAACATAGAT GTGGTCAAGT TTCTTCTTGA AAAAGGAGCT 
AACGTTAATG CAACAGATAA CTTTCTGTGG ACTCCACTTC ATTTTGCATG CCATGCAGGC 
CAACAAGACA TTGTTGAGCT TCTTGTTGAA TCTGGAGCTT TAATAGATGC AGCTTCAATC 
AACAACTCAA CTCCTTTAAA TAGAGCCATT GAAAGCTGCA GACTGGATAC AGTAAAATAC 
CTACTTGATA TTGGTGCTAA ATTCCAGCTG GAAAATAGAA AAGGGCATAG TGCCATGGAC 
GTTGCAAAGG CATATGCTGA TTATAGAATA ATTGATCTGA TTAAAGAAAA GCTAGATAAC 
TTGCCGAAAC CAGCAGAAAA TCAAAAACTA AAAGGCAAGA CACCTCCTAT ACTGAAGACT 
GAAGGCCCTG AAATTAAGAA AGAAGAGGAA CTGCTGTCAT CAATTTATGG TGTACCAACC 
ACATCAGAGG GAAAGAAAGT ACAGAAGGGT AATGTGGTTC ATCTGAATTC ATTGATTACC 
AGTGGTTATA CTAAGAAAGT GGATATCACA TTTATTCCAC GGAGGATTTG GAGTCCTGAA 
GCCACAACAG CAGAGCTGAT CAGGAAGAGG GAACTACGGC GAGAGAGGTT TACACATGAG 
GTGGACTTCG ACGATTTTAT GATGCCTTTT CAGAAGAACA TCACAGAGAA AGCTCGAGCA 
CTGGAAGCTG CCTTGAAGAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005260849.1
CDS	480..2243
Translation	MEASREGVVEIVRGILERGGEVNAFDNDRHHAAHFAAKGGFFDILKLLFAYNGDVGLISINGNTPLHYAAMGGFADCCKYIAQRGCDLKWKNLDHKTPRAVAKEGGFKAASKEIRRAERIANKLARPGAKNPNPLWALRLHDWSVEREAFLREAFAVLDRGDGSISKNDFVMVLEERQDYASSEQLAAIAHLHEKTRGGGVNINEFFKGTRYLNKSFVLGSYGPKKKEKGMGKKGKKGKFVLPLPICVIPEYAFPRRQDGGPPYYMIETYKNVTDSSRFNRDHPPEHPIQDDSVWYIDDSEKVFSNINIITKAGDLASLKKAFESGIPVDMKDNYYKTPLMTACASGNIDVVKFLLEKGANVNATDNFLWTPLHFACHAGQQDIVELLVESGALIDAASINNSTPLNRAIESCRLDTVKYLLDIGAKFQLENRKGHSAMDVAKAYADYRIIDLIKEKLDNLPKPAENQKLKGKTPPILKTEGPEIKKEEELLSSIYGVPTTSEGKKVQKGNVVHLNSLITSGYTKKVDITFIPRRIWSPEATTAELIRKRELRRERFTHEVDFDDFMMPFQKNITEKARALEAALKT

Target ORF information:

RefSeq Version	XM_005260792.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260792.2

ORF Insert Sequence:

ATGGAAGCGT CAAGAGAAGG GGTAGTGGAA ATAGTTCGAG GCATATTGGA AAGAGGAGGT 
GAAGTGAATG CATTTGACAA CGACAGGCAT CACGCTGCTC ATTTTGCTGC TAAAGGAGGC 
TTTTTCGATA TATTGAAGCT TCTTTTTGCC TACAATGGAG ACGTGGGGCT GATTTCGATA 
AATGGGAACA CACCACTTCA TTATGCTGCC ATGGGTGGTT TTGCAGACTG CTGTAAATAT 
ATAGCTCAGC GAGGATGTGA CCTGAAATGG AAGAATTTAG ATCATAAAAC GCCCAGGGCT 
GTGGCTAAGG AAGGCGGCTT CAAAGCAGCA AGCAAAGAAA TACGCCGAGC AGAGAGAATC 
GCTAATAAAC TAGCCAGGCC AGGAGCCAAA AATCCAAATC CACTGTGGGC CCTTAGACTG 
CACGATTGGT CCGTAGAACG TGAGGCTTTC CTCCGGGAAG CCTTTGCGGT TTTAGACAGG 
GGTGATGGAA GCATCAGCAA GAACGACTTC GTGATGGTGT TGGAGGAAAG GCAGGATTAT 
GCAAGCTCAG AACAGCTGGC TGCCATCGCT CACCTTCATG AGAAAACCCG GGGAGGAGGG 
GTCAATATTA ATGAATTCTT TAAAGGAACC AGATATTTAA ACAAGTCTTT TGTCTTAGGA 
TCGTATGGAC CTAAGAAAAA GGAAAAAGGG ATGGGCAAAA AAGGAAAGAA AGGGAAATTT 
GTCTTACCCC TTCCAATCTG TGTCATTCCT GAGTACGCGT TTCCACGCCG GCAGGATGGT 
GGGCCACCGT ATTACATGAT TGAGACCTAC AAGAATGTCA CTGATAGCAG CCGGTTTAAT 
AGAGATCATC CCCCAGAACA TCCCATTCAG GATGACTCTG TTTGGTACAT TGATGATTCA 
GAGAAGGTAT TTTCAAACAT TAATATTATC ACCAAAGCAG GGGATCTGGC TTCTCTGAAA 
AAGGCCTTTG AATCAGGAAT ACCTGTGGAT ATGAAGGATA ATTATTACAA AACTCCGCTA 
ATGACGGCGT GTGCAAGTGG AAACATAGAT GTGGTCAAGT TTCTTCTTGA AAAAGGAGCT 
AACGTTAATG CAACAGATAA CTTTCTGTGG ACTCCACTTC ATTTTGCATG CCATGCAGGC 
CAACAAGACA TTGTTGAGCT TCTTGTTGAA TCTGGAGCTT TAATAGATGC AGCTTCAATC 
AACAACTCAA CTCCTTTAAA TAGAGCCATT GAAAGCTGCA GACTGGATAC AGTAAAATAC 
CTACTTGATA TTGGTGCTAA ATTCCAGCTG GAAAATAGAA AAGGGCATAG TGCCATGGAC 
GTTGCAAAGG CATATGCTGA TTATAGAATA ATTGATCTGA TTAAAGAAAA GCTAGATAAC 
TTGCCGAAAC CAGCAGAAAA TCAAAAACTA AAAGGCAAGA CACCTCCTAT ACTGAAGACT 
GAAGGCCCTG AAATTAAGAA AGAAGAGGAA CTGCTGTCAT CAATTTATGG TGTACCAACC 
ACATCAGAGG GAAAGAAAGT ACAGAAGGGT AATGTGGTTC ATCTGAATTC ATTGATTACC 
AGTGGTTATA CTAAGAAAGT GGATATCACA TTTATTCCAC GGAGGATTTG GAGTCCTGAA 
GCCACAACAG CAGAGCTGAT CAGGAAGAGG GAACTACGGC GAGAGAGGTT TACACATGAG 
GTGGACTTCG ACGATTTTAT GATGCCTTTT CAGAAGAACA TCACAGAGAA AGCTCGAGCA 
CTGGAAGCTG CCTTGAAGAC CTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKEF1 ( NM_198798.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_942093.1 Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 2, mRNA.

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CloneID	OHu03802
Clone ID Related Accession (Same CDS sequence)	NM_198798.2 , NM_198798.3 , NM_022096.6 , NM_022096.5
Accession Version	NM_198798.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	ankyrin repeat and EF-hand domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP279314.1, AK097689.1, AK025322.1, AL109754.13 and BU621946.1. On Jan 7, 2015 this sequence version replaced NM_198798.1. Transcript Variant: This variant (2) lacks an exon in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660805.85060.1, SRR1660803.166101.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTAG CAGATAAGAG ACTTGAGAAC TTACAGATCT ACAAAGTTCT TCAATGTGTG 
CGGAACAAAG ACAAGAAGCA GATAGAGAAG CTGACCAAGC TTGGATACCC TGAACTAATC 
AATTATACAG AACCCATTAA TGGACTTAGT GCTTTGCACT TAGCCTCAGT TTCCAATGAT 
ATTGATATGG TCAGCTTTCT CCTTGACCTT GGTGCTCACC CTGATGTGCA AGACCGAATG 
GGCTGTACTC CCACAATGAG GGCTGCAGAA CTGGGCCATG AATTGTCAAT GGAAATATTA 
GCAAAGGCAA AGGCTGATAT GACTATAGTT GATAATGAAG GAAAAGGTGT TTTGTTTTAC 
TGCATTTTAC CGACTAAGCG GCATTATCGC TGTGCTCTGA TCGCCCTTGA ACATGGTGCA 
GATGTCAACA ATTCTACCTA TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGATGCA 
CATGATGTTA AAGATGTGTG CCTGACATTT TTGGAAAAAG GAGCCAATCC TAATGCAATC 
AACTCATCCA CAGGCCGCAC AGCTTTAATG GAAGCGTCAA GAGAAGGGGT AGTGGAAATA 
GTTCGAGGCA TATTGGAAAG AGGAGGTGAA GTGAATGCAT TTGACAACGA CAGGCATCAC 
GCTGCTCATT TTGCTGCTAA AGGAGGCTTT TTCGATATAT TGAAGCTTCT TTTTGCCTAC 
AATGGAGACG TGGGGCTGAT TTCGATAAAT GGGAACACAC CACTTCATTA TGCTGCCATG 
GGTGGTTTTG CAGACTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT GAAATGGAAG 
AATTTAGATC ATAAAACGCC CAGGGCTGTG GCTAAGGAAG GCGGCTTCAA AGCAGCAAGC 
AAAGAAATAC GCCGAGCAGA GAGAATCGCT AATAAACTAG CCAGGCCAGG AGCCAAAAAT 
CCAAATCCAC TGTGGGCCCT TAGACTGCAC GATTGGTCCG TAGAACGTGA GGCTTTCCTC 
CGGGAAGCCT TTGCGGTTTT AGACAGGGGT GATGGAAGCA TCAGCAAGAA CGACTTCGTG 
ATGGTGTTGG AGGAAAGGCA GGATTATGCA AGCTCAGAAC AGCTGGCTGC CATCGCTCAC 
CTTCATGAGA AAACCCGGGG AGGAGGGGTC AATATTAATG AATTCTTTAA AGGAACCAGA 
TATTTAAACA AGTCTTTTGT CTTAGGATCG TATGGACCTA AGAAAAAGGA AAAAGGGATG 
GGCAAAAAAG GAAAGAAAGG GAAATTTGTC TTACCCCTTC CAATCTGTGT CATTCCTGAG 
TACGCGTTTC CACGCCGGCA GGATGGTGGG CCACCGTATT ACATGATTGA GACCTACAAG 
AATGTCACTG ATAGCAGCCG GTTTAATAGA GATCATCCCC CAGAACATCC CATTCAGGAT 
GACTCTGTTT GGTACATTGA TGATTCAGAG AAGGTATTTT CAAACATTAA TATTATCACC 
AAAGCAGGGG ATCTGGCTTC TCTGAAAAAG GCCTTTGAAT CAGGAATACC TGTGGATATG 
AAGGATAATT ATTACAAAAC TCCGCTAATG ACGGCGTGTG CAAGTGGAAA CATAGATGTG 
GTCAAGTTTC TTCTTGAAAA AGGAGCTAAC GTTAATGCAA CAGATAACTT TCTGTGGACT 
CCACTTCATT TTGCATGCCA TGCAGGCCAA CAAGACATTG TTGAGCTTCT TGTTGAATCT 
GGAGCTTTAA TAGATGCAGC TTCAATCAAC AACTCAACTC CTTTAAATAG AGCCATTGAA 
AGCTGCAGAC TGGATACAGT AAAATACCTA CTTGATATTG GTGCTAAATT CCAGCTGGAA 
AATAGAAAAG GGCATAGTGC CATGGACGTT GCAAAGGCAT ATGCTGATTA TAGAATAATT 
GATCTGATTA AAGAAAAGCT AGATAACTTG CCGAAACCAG CAGAAAATCA AAAACTAAAA 
GGCAAGACAC CTCCTATACT GAAGACTGAA GGCCCTGAAA TTAAGAAAGA AGAGGAACTG 
CTGTCATCAA TTTATGGTGT ACCAACCACA TCAGAGGGAA AGAAAGTACA GAAGGGTAAT 
GTGGTTCATC TGAATTCATT GATTACCAGT GGTTATACTA AGAAAGTGGA TATCACATTT 
ATTCCACGGA GGATTTGGAG TCCTGAAGCC ACAACAGCAG AGCTGATCAG GAAGAGGGAA 
CTACGGCGAG AGAGGTTTAC ACATGAGGTG GACTTCGACG ATTTTATGAT GCCTTTTCAG 
AAGAACATCA CAGAGAAAGC TCGAGCACTG GAAGCTGCCT TGAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942093.1
CDS	380..2710
Translation	MALADKRLENLQIYKVLQCVRNKDKKQIEKLTKLGYPELINYTEPINGLSALHLASVSNDIDMVSFLLDLGAHPDVQDRMGCTPTMRAAELGHELSMEILAKAKADMTIVDNEGKGVLFYCILPTKRHYRCALIALEHGADVNNSTYEGKPIFLRACEDAHDVKDVCLTFLEKGANPNAINSSTGRTALMEASREGVVEIVRGILERGGEVNAFDNDRHHAAHFAAKGGFFDILKLLFAYNGDVGLISINGNTPLHYAAMGGFADCCKYIAQRGCDLKWKNLDHKTPRAVAKEGGFKAASKEIRRAERIANKLARPGAKNPNPLWALRLHDWSVEREAFLREAFAVLDRGDGSISKNDFVMVLEERQDYASSEQLAAIAHLHEKTRGGGVNINEFFKGTRYLNKSFVLGSYGPKKKEKGMGKKGKKGKFVLPLPICVIPEYAFPRRQDGGPPYYMIETYKNVTDSSRFNRDHPPEHPIQDDSVWYIDDSEKVFSNINIITKAGDLASLKKAFESGIPVDMKDNYYKTPLMTACASGNIDVVKFLLEKGANVNATDNFLWTPLHFACHAGQQDIVELLVESGALIDAASINNSTPLNRAIESCRLDTVKYLLDIGAKFQLENRKGHSAMDVAKAYADYRIIDLIKEKLDNLPKPAENQKLKGKTPPILKTEGPEIKKEEELLSSIYGVPTTSEGKKVQKGNVVHLNSLITSGYTKKVDITFIPRRIWSPEATTAELIRKRELRRERFTHEVDFDDFMMPFQKNITEKARALEAALKT

Target ORF information:

RefSeq Version	NM_198798.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198798.2

ORF Insert Sequence:

ATGGCTTTAG CAGATAAGAG ACTTGAGAAC TTACAGATCT ACAAAGTTCT TCAATGTGTG 
CGGAACAAAG ACAAGAAGCA GATAGAGAAG CTGACCAAGC TTGGATACCC TGAACTAATC 
AATTATACAG AACCCATTAA TGGACTTAGT GCTTTGCACT TAGCCTCAGT TTCCAATGAT 
ATTGATATGG TCAGCTTTCT CCTTGACCTT GGTGCTCACC CTGATGTGCA AGACCGAATG 
GGCTGTACTC CCACAATGAG GGCTGCAGAA CTGGGCCATG AATTGTCAAT GGAAATATTA 
GCAAAGGCAA AGGCTGATAT GACTATAGTT GATAATGAAG GAAAAGGTGT TTTGTTTTAC 
TGCATTTTAC CGACTAAGCG GCATTATCGC TGTGCTCTGA TCGCCCTTGA ACATGGTGCA 
GATGTCAACA ATTCTACCTA TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGATGCA 
CATGATGTTA AAGATGTGTG CCTGACATTT TTGGAAAAAG GAGCCAATCC TAATGCAATC 
AACTCATCCA CAGGCCGCAC AGCTTTAATG GAAGCGTCAA GAGAAGGGGT AGTGGAAATA 
GTTCGAGGCA TATTGGAAAG AGGAGGTGAA GTGAATGCAT TTGACAACGA CAGGCATCAC 
GCTGCTCATT TTGCTGCTAA AGGAGGCTTT TTCGATATAT TGAAGCTTCT TTTTGCCTAC 
AATGGAGACG TGGGGCTGAT TTCGATAAAT GGGAACACAC CACTTCATTA TGCTGCCATG 
GGTGGTTTTG CAGACTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT GAAATGGAAG 
AATTTAGATC ATAAAACGCC CAGGGCTGTG GCTAAGGAAG GCGGCTTCAA AGCAGCAAGC 
AAAGAAATAC GCCGAGCAGA GAGAATCGCT AATAAACTAG CCAGGCCAGG AGCCAAAAAT 
CCAAATCCAC TGTGGGCCCT TAGACTGCAC GATTGGTCCG TAGAACGTGA GGCTTTCCTC 
CGGGAAGCCT TTGCGGTTTT AGACAGGGGT GATGGAAGCA TCAGCAAGAA CGACTTCGTG 
ATGGTGTTGG AGGAAAGGCA GGATTATGCA AGCTCAGAAC AGCTGGCTGC CATCGCTCAC 
CTTCATGAGA AAACCCGGGG AGGAGGGGTC AATATTAATG AATTCTTTAA AGGAACCAGA 
TATTTAAACA AGTCTTTTGT CTTAGGATCG TATGGACCTA AGAAAAAGGA AAAAGGGATG 
GGCAAAAAAG GAAAGAAAGG GAAATTTGTC TTACCCCTTC CAATCTGTGT CATTCCTGAG 
TACGCGTTTC CACGCCGGCA GGATGGTGGG CCACCGTATT ACATGATTGA GACCTACAAG 
AATGTCACTG ATAGCAGCCG GTTTAATAGA GATCATCCCC CAGAACATCC CATTCAGGAT 
GACTCTGTTT GGTACATTGA TGATTCAGAG AAGGTATTTT CAAACATTAA TATTATCACC 
AAAGCAGGGG ATCTGGCTTC TCTGAAAAAG GCCTTTGAAT CAGGAATACC TGTGGATATG 
AAGGATAATT ATTACAAAAC TCCGCTAATG ACGGCGTGTG CAAGTGGAAA CATAGATGTG 
GTCAAGTTTC TTCTTGAAAA AGGAGCTAAC GTTAATGCAA CAGATAACTT TCTGTGGACT 
CCACTTCATT TTGCATGCCA TGCAGGCCAA CAAGACATTG TTGAGCTTCT TGTTGAATCT 
GGAGCTTTAA TAGATGCAGC TTCAATCAAC AACTCAACTC CTTTAAATAG AGCCATTGAA 
AGCTGCAGAC TGGATACAGT AAAATACCTA CTTGATATTG GTGCTAAATT CCAGCTGGAA 
AATAGAAAAG GGCATAGTGC CATGGACGTT GCAAAGGCAT ATGCTGATTA TAGAATAATT 
GATCTGATTA AAGAAAAGCT AGATAACTTG CCGAAACCAG CAGAAAATCA AAAACTAAAA 
GGCAAGACAC CTCCTATACT GAAGACTGAA GGCCCTGAAA TTAAGAAAGA AGAGGAACTG 
CTGTCATCAA TTTATGGTGT ACCAACCACA TCAGAGGGAA AGAAAGTACA GAAGGGTAAT 
GTGGTTCATC TGAATTCATT GATTACCAGT GGTTATACTA AGAAAGTGGA TATCACATTT 
ATTCCACGGA GGATTTGGAG TCCTGAAGCC ACAACAGCAG AGCTGATCAG GAAGAGGGAA 
CTACGGCGAG AGAGGTTTAC ACATGAGGTG GACTTCGACG ATTTTATGAT GCCTTTTCAG 
AAGAACATCA CAGAGAAAGC TCGAGCACTG GAAGCTGCCT TGAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKEF1 ( NM_198798.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_942093.1 Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 2, mRNA.

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CloneID	OHu03802
Clone ID Related Accession (Same CDS sequence)	NM_198798.2 , NM_198798.3 , NM_022096.6 , NM_022096.5
Accession Version	NM_198798.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-30
Organism	Homo sapiens(human)
Product	ankyrin repeat and EF-hand domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL109754.13, BP279314.1, AK097689.1, AK025322.1 and BU621946.1. On May 31, 2019 this sequence version replaced NM_198798.2. Transcript Variant: This variant (2) lacks an exon in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660803.166101.1, SRR1660805.85060.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTAG CAGATAAGAG ACTTGAGAAC TTACAGATCT ACAAAGTTCT TCAATGTGTG 
CGGAACAAAG ACAAGAAGCA GATAGAGAAG CTGACCAAGC TTGGATACCC TGAACTAATC 
AATTATACAG AACCCATTAA TGGACTTAGT GCTTTGCACT TAGCCTCAGT TTCCAATGAT 
ATTGATATGG TCAGCTTTCT CCTTGACCTT GGTGCTCACC CTGATGTGCA AGACCGAATG 
GGCTGTACTC CCACAATGAG GGCTGCAGAA CTGGGCCATG AATTGTCAAT GGAAATATTA 
GCAAAGGCAA AGGCTGATAT GACTATAGTT GATAATGAAG GAAAAGGTGT TTTGTTTTAC 
TGCATTTTAC CGACTAAGCG GCATTATCGC TGTGCTCTGA TCGCCCTTGA ACATGGTGCA 
GATGTCAACA ATTCTACCTA TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGATGCA 
CATGATGTTA AAGATGTGTG CCTGACATTT TTGGAAAAAG GAGCCAATCC TAATGCAATC 
AACTCATCCA CAGGCCGCAC AGCTTTAATG GAAGCGTCAA GAGAAGGGGT AGTGGAAATA 
GTTCGAGGCA TATTGGAAAG AGGAGGTGAA GTGAATGCAT TTGACAACGA CAGGCATCAC 
GCTGCTCATT TTGCTGCTAA AGGAGGCTTT TTCGATATAT TGAAGCTTCT TTTTGCCTAC 
AATGGAGACG TGGGGCTGAT TTCGATAAAT GGGAACACAC CACTTCATTA TGCTGCCATG 
GGTGGTTTTG CAGACTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT GAAATGGAAG 
AATTTAGATC ATAAAACGCC CAGGGCTGTG GCTAAGGAAG GCGGCTTCAA AGCAGCAAGC 
AAAGAAATAC GCCGAGCAGA GAGAATCGCT AATAAACTAG CCAGGCCAGG AGCCAAAAAT 
CCAAATCCAC TGTGGGCCCT TAGACTGCAC GATTGGTCCG TAGAACGTGA GGCTTTCCTC 
CGGGAAGCCT TTGCGGTTTT AGACAGGGGT GATGGAAGCA TCAGCAAGAA CGACTTCGTG 
ATGGTGTTGG AGGAAAGGCA GGATTATGCA AGCTCAGAAC AGCTGGCTGC CATCGCTCAC 
CTTCATGAGA AAACCCGGGG AGGAGGGGTC AATATTAATG AATTCTTTAA AGGAACCAGA 
TATTTAAACA AGTCTTTTGT CTTAGGATCG TATGGACCTA AGAAAAAGGA AAAAGGGATG 
GGCAAAAAAG GAAAGAAAGG GAAATTTGTC TTACCCCTTC CAATCTGTGT CATTCCTGAG 
TACGCGTTTC CACGCCGGCA GGATGGTGGG CCACCGTATT ACATGATTGA GACCTACAAG 
AATGTCACTG ATAGCAGCCG GTTTAATAGA GATCATCCCC CAGAACATCC CATTCAGGAT 
GACTCTGTTT GGTACATTGA TGATTCAGAG AAGGTATTTT CAAACATTAA TATTATCACC 
AAAGCAGGGG ATCTGGCTTC TCTGAAAAAG GCCTTTGAAT CAGGAATACC TGTGGATATG 
AAGGATAATT ATTACAAAAC TCCGCTAATG ACGGCGTGTG CAAGTGGAAA CATAGATGTG 
GTCAAGTTTC TTCTTGAAAA AGGAGCTAAC GTTAATGCAA CAGATAACTT TCTGTGGACT 
CCACTTCATT TTGCATGCCA TGCAGGCCAA CAAGACATTG TTGAGCTTCT TGTTGAATCT 
GGAGCTTTAA TAGATGCAGC TTCAATCAAC AACTCAACTC CTTTAAATAG AGCCATTGAA 
AGCTGCAGAC TGGATACAGT AAAATACCTA CTTGATATTG GTGCTAAATT CCAGCTGGAA 
AATAGAAAAG GGCATAGTGC CATGGACGTT GCAAAGGCAT ATGCTGATTA TAGAATAATT 
GATCTGATTA AAGAAAAGCT AGATAACTTG CCGAAACCAG CAGAAAATCA AAAACTAAAA 
GGCAAGACAC CTCCTATACT GAAGACTGAA GGCCCTGAAA TTAAGAAAGA AGAGGAACTG 
CTGTCATCAA TTTATGGTGT ACCAACCACA TCAGAGGGAA AGAAAGTACA GAAGGGTAAT 
GTGGTTCATC TGAATTCATT GATTACCAGT GGTTATACTA AGAAAGTGGA TATCACATTT 
ATTCCACGGA GGATTTGGAG TCCTGAAGCC ACAACAGCAG AGCTGATCAG GAAGAGGGAA 
CTACGGCGAG AGAGGTTTAC ACATGAGGTG GACTTCGACG ATTTTATGAT GCCTTTTCAG 
AAGAACATCA CAGAGAAAGC TCGAGCACTG GAAGCTGCCT TGAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_942093.1
CDS	392..2722
Translation	MALADKRLENLQIYKVLQCVRNKDKKQIEKLTKLGYPELINYTEPINGLSALHLASVSNDIDMVSFLLDLGAHPDVQDRMGCTPTMRAAELGHELSMEILAKAKADMTIVDNEGKGVLFYCILPTKRHYRCALIALEHGADVNNSTYEGKPIFLRACEDAHDVKDVCLTFLEKGANPNAINSSTGRTALMEASREGVVEIVRGILERGGEVNAFDNDRHHAAHFAAKGGFFDILKLLFAYNGDVGLISINGNTPLHYAAMGGFADCCKYIAQRGCDLKWKNLDHKTPRAVAKEGGFKAASKEIRRAERIANKLARPGAKNPNPLWALRLHDWSVEREAFLREAFAVLDRGDGSISKNDFVMVLEERQDYASSEQLAAIAHLHEKTRGGGVNINEFFKGTRYLNKSFVLGSYGPKKKEKGMGKKGKKGKFVLPLPICVIPEYAFPRRQDGGPPYYMIETYKNVTDSSRFNRDHPPEHPIQDDSVWYIDDSEKVFSNINIITKAGDLASLKKAFESGIPVDMKDNYYKTPLMTACASGNIDVVKFLLEKGANVNATDNFLWTPLHFACHAGQQDIVELLVESGALIDAASINNSTPLNRAIESCRLDTVKYLLDIGAKFQLENRKGHSAMDVAKAYADYRIIDLIKEKLDNLPKPAENQKLKGKTPPILKTEGPEIKKEEELLSSIYGVPTTSEGKKVQKGNVVHLNSLITSGYTKKVDITFIPRRIWSPEATTAELIRKRELRRERFTHEVDFDDFMMPFQKNITEKARALEAALKT

Target ORF information:

RefSeq Version	NM_198798.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_198798.3

ORF Insert Sequence:

ATGGCTTTAG CAGATAAGAG ACTTGAGAAC TTACAGATCT ACAAAGTTCT TCAATGTGTG 
CGGAACAAAG ACAAGAAGCA GATAGAGAAG CTGACCAAGC TTGGATACCC TGAACTAATC 
AATTATACAG AACCCATTAA TGGACTTAGT GCTTTGCACT TAGCCTCAGT TTCCAATGAT 
ATTGATATGG TCAGCTTTCT CCTTGACCTT GGTGCTCACC CTGATGTGCA AGACCGAATG 
GGCTGTACTC CCACAATGAG GGCTGCAGAA CTGGGCCATG AATTGTCAAT GGAAATATTA 
GCAAAGGCAA AGGCTGATAT GACTATAGTT GATAATGAAG GAAAAGGTGT TTTGTTTTAC 
TGCATTTTAC CGACTAAGCG GCATTATCGC TGTGCTCTGA TCGCCCTTGA ACATGGTGCA 
GATGTCAACA ATTCTACCTA TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGATGCA 
CATGATGTTA AAGATGTGTG CCTGACATTT TTGGAAAAAG GAGCCAATCC TAATGCAATC 
AACTCATCCA CAGGCCGCAC AGCTTTAATG GAAGCGTCAA GAGAAGGGGT AGTGGAAATA 
GTTCGAGGCA TATTGGAAAG AGGAGGTGAA GTGAATGCAT TTGACAACGA CAGGCATCAC 
GCTGCTCATT TTGCTGCTAA AGGAGGCTTT TTCGATATAT TGAAGCTTCT TTTTGCCTAC 
AATGGAGACG TGGGGCTGAT TTCGATAAAT GGGAACACAC CACTTCATTA TGCTGCCATG 
GGTGGTTTTG CAGACTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT GAAATGGAAG 
AATTTAGATC ATAAAACGCC CAGGGCTGTG GCTAAGGAAG GCGGCTTCAA AGCAGCAAGC 
AAAGAAATAC GCCGAGCAGA GAGAATCGCT AATAAACTAG CCAGGCCAGG AGCCAAAAAT 
CCAAATCCAC TGTGGGCCCT TAGACTGCAC GATTGGTCCG TAGAACGTGA GGCTTTCCTC 
CGGGAAGCCT TTGCGGTTTT AGACAGGGGT GATGGAAGCA TCAGCAAGAA CGACTTCGTG 
ATGGTGTTGG AGGAAAGGCA GGATTATGCA AGCTCAGAAC AGCTGGCTGC CATCGCTCAC 
CTTCATGAGA AAACCCGGGG AGGAGGGGTC AATATTAATG AATTCTTTAA AGGAACCAGA 
TATTTAAACA AGTCTTTTGT CTTAGGATCG TATGGACCTA AGAAAAAGGA AAAAGGGATG 
GGCAAAAAAG GAAAGAAAGG GAAATTTGTC TTACCCCTTC CAATCTGTGT CATTCCTGAG 
TACGCGTTTC CACGCCGGCA GGATGGTGGG CCACCGTATT ACATGATTGA GACCTACAAG 
AATGTCACTG ATAGCAGCCG GTTTAATAGA GATCATCCCC CAGAACATCC CATTCAGGAT 
GACTCTGTTT GGTACATTGA TGATTCAGAG AAGGTATTTT CAAACATTAA TATTATCACC 
AAAGCAGGGG ATCTGGCTTC TCTGAAAAAG GCCTTTGAAT CAGGAATACC TGTGGATATG 
AAGGATAATT ATTACAAAAC TCCGCTAATG ACGGCGTGTG CAAGTGGAAA CATAGATGTG 
GTCAAGTTTC TTCTTGAAAA AGGAGCTAAC GTTAATGCAA CAGATAACTT TCTGTGGACT 
CCACTTCATT TTGCATGCCA TGCAGGCCAA CAAGACATTG TTGAGCTTCT TGTTGAATCT 
GGAGCTTTAA TAGATGCAGC TTCAATCAAC AACTCAACTC CTTTAAATAG AGCCATTGAA 
AGCTGCAGAC TGGATACAGT AAAATACCTA CTTGATATTG GTGCTAAATT CCAGCTGGAA 
AATAGAAAAG GGCATAGTGC CATGGACGTT GCAAAGGCAT ATGCTGATTA TAGAATAATT 
GATCTGATTA AAGAAAAGCT AGATAACTTG CCGAAACCAG CAGAAAATCA AAAACTAAAA 
GGCAAGACAC CTCCTATACT GAAGACTGAA GGCCCTGAAA TTAAGAAAGA AGAGGAACTG 
CTGTCATCAA TTTATGGTGT ACCAACCACA TCAGAGGGAA AGAAAGTACA GAAGGGTAAT 
GTGGTTCATC TGAATTCATT GATTACCAGT GGTTATACTA AGAAAGTGGA TATCACATTT 
ATTCCACGGA GGATTTGGAG TCCTGAAGCC ACAACAGCAG AGCTGATCAG GAAGAGGGAA 
CTACGGCGAG AGAGGTTTAC ACATGAGGTG GACTTCGACG ATTTTATGAT GCCTTTTCAG 
AAGAACATCA CAGAGAAAGC TCGAGCACTG GAAGCTGCCT TGAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ANKEF1 ( NM_022096.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_071379.3 Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu03802
Clone ID Related Accession (Same CDS sequence)	NM_198798.2 , NM_198798.3 , NM_022096.6 , NM_022096.5
Accession Version	NM_022096.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2331bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	ankyrin repeat and EF-hand domain-containing protein 1 isoform 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BP279314.1, BI562273.1, AK097689.1, AK025322.1, AL109754.13 and BU621946.1. On Jan 7, 2015 this sequence version replaced NM_022096.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: SRR1660809.84743.1, BC022878.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCTTTAG CAGATAAGAG ACTTGAGAAC TTACAGATCT ACAAAGTTCT TCAATGTGTG 
CGGAACAAAG ACAAGAAGCA GATAGAGAAG CTGACCAAGC TTGGATACCC TGAACTAATC 
AATTATACAG AACCCATTAA TGGACTTAGT GCTTTGCACT TAGCCTCAGT TTCCAATGAT 
ATTGATATGG TCAGCTTTCT CCTTGACCTT GGTGCTCACC CTGATGTGCA AGACCGAATG 
GGCTGTACTC CCACAATGAG GGCTGCAGAA CTGGGCCATG AATTGTCAAT GGAAATATTA 
GCAAAGGCAA AGGCTGATAT GACTATAGTT GATAATGAAG GAAAAGGTGT TTTGTTTTAC 
TGCATTTTAC CGACTAAGCG GCATTATCGC TGTGCTCTGA TCGCCCTTGA ACATGGTGCA 
GATGTCAACA ATTCTACCTA TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGATGCA 
CATGATGTTA AAGATGTGTG CCTGACATTT TTGGAAAAAG GAGCCAATCC TAATGCAATC 
AACTCATCCA CAGGCCGCAC AGCTTTAATG GAAGCGTCAA GAGAAGGGGT AGTGGAAATA 
GTTCGAGGCA TATTGGAAAG AGGAGGTGAA GTGAATGCAT TTGACAACGA CAGGCATCAC 
GCTGCTCATT TTGCTGCTAA AGGAGGCTTT TTCGATATAT TGAAGCTTCT TTTTGCCTAC 
AATGGAGACG TGGGGCTGAT TTCGATAAAT GGGAACACAC CACTTCATTA TGCTGCCATG 
GGTGGTTTTG CAGACTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT GAAATGGAAG 
AATTTAGATC ATAAAACGCC CAGGGCTGTG GCTAAGGAAG GCGGCTTCAA AGCAGCAAGC 
AAAGAAATAC GCCGAGCAGA GAGAATCGCT AATAAACTAG CCAGGCCAGG AGCCAAAAAT 
CCAAATCCAC TGTGGGCCCT TAGACTGCAC GATTGGTCCG TAGAACGTGA GGCTTTCCTC 
CGGGAAGCCT TTGCGGTTTT AGACAGGGGT GATGGAAGCA TCAGCAAGAA CGACTTCGTG 
ATGGTGTTGG AGGAAAGGCA GGATTATGCA AGCTCAGAAC AGCTGGCTGC CATCGCTCAC 
CTTCATGAGA AAACCCGGGG AGGAGGGGTC AATATTAATG AATTCTTTAA AGGAACCAGA 
TATTTAAACA AGTCTTTTGT CTTAGGATCG TATGGACCTA AGAAAAAGGA AAAAGGGATG 
GGCAAAAAAG GAAAGAAAGG GAAATTTGTC TTACCCCTTC CAATCTGTGT CATTCCTGAG 
TACGCGTTTC CACGCCGGCA GGATGGTGGG CCACCGTATT ACATGATTGA GACCTACAAG 
AATGTCACTG ATAGCAGCCG GTTTAATAGA GATCATCCCC CAGAACATCC CATTCAGGAT 
GACTCTGTTT GGTACATTGA TGATTCAGAG AAGGTATTTT CAAACATTAA TATTATCACC 
AAAGCAGGGG ATCTGGCTTC TCTGAAAAAG GCCTTTGAAT CAGGAATACC TGTGGATATG 
AAGGATAATT ATTACAAAAC TCCGCTAATG ACGGCGTGTG CAAGTGGAAA CATAGATGTG 
GTCAAGTTTC TTCTTGAAAA AGGAGCTAAC GTTAATGCAA CAGATAACTT TCTGTGGACT 
CCACTTCATT TTGCATGCCA TGCAGGCCAA CAAGACATTG TTGAGCTTCT TGTTGAATCT 
GGAGCTTTAA TAGATGCAGC TTCAATCAAC AACTCAACTC CTTTAAATAG AGCCATTGAA 
AGCTGCAGAC TGGATACAGT AAAATACCTA CTTGATATTG GTGCTAAATT CCAGCTGGAA 
AATAGAAAAG GGCATAGTGC CATGGACGTT GCAAAGGCAT ATGCTGATTA TAGAATAATT 
GATCTGATTA AAGAAAAGCT AGATAACTTG CCGAAACCAG CAGAAAATCA AAAACTAAAA 
GGCAAGACAC CTCCTATACT GAAGACTGAA GGCCCTGAAA TTAAGAAAGA AGAGGAACTG 
CTGTCATCAA TTTATGGTGT ACCAACCACA TCAGAGGGAA AGAAAGTACA GAAGGGTAAT 
GTGGTTCATC TGAATTCATT GATTACCAGT GGTTATACTA AGAAAGTGGA TATCACATTT 
ATTCCACGGA GGATTTGGAG TCCTGAAGCC ACAACAGCAG AGCTGATCAG GAAGAGGGAA 
CTACGGCGAG AGAGGTTTAC ACATGAGGTG GACTTCGACG ATTTTATGAT GCCTTTTCAG 
AAGAACATCA CAGAGAAAGC TCGAGCACTG GAAGCTGCCT TGAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071379.3
CDS	444..2774
Translation	MALADKRLENLQIYKVLQCVRNKDKKQIEKLTKLGYPELINYTEPINGLSALHLASVSNDIDMVSFLLDLGAHPDVQDRMGCTPTMRAAELGHELSMEILAKAKADMTIVDNEGKGVLFYCILPTKRHYRCALIALEHGADVNNSTYEGKPIFLRACEDAHDVKDVCLTFLEKGANPNAINSSTGRTALMEASREGVVEIVRGILERGGEVNAFDNDRHHAAHFAAKGGFFDILKLLFAYNGDVGLISINGNTPLHYAAMGGFADCCKYIAQRGCDLKWKNLDHKTPRAVAKEGGFKAASKEIRRAERIANKLARPGAKNPNPLWALRLHDWSVEREAFLREAFAVLDRGDGSISKNDFVMVLEERQDYASSEQLAAIAHLHEKTRGGGVNINEFFKGTRYLNKSFVLGSYGPKKKEKGMGKKGKKGKFVLPLPICVIPEYAFPRRQDGGPPYYMIETYKNVTDSSRFNRDHPPEHPIQDDSVWYIDDSEKVFSNINIITKAGDLASLKKAFESGIPVDMKDNYYKTPLMTACASGNIDVVKFLLEKGANVNATDNFLWTPLHFACHAGQQDIVELLVESGALIDAASINNSTPLNRAIESCRLDTVKYLLDIGAKFQLENRKGHSAMDVAKAYADYRIIDLIKEKLDNLPKPAENQKLKGKTPPILKTEGPEIKKEEELLSSIYGVPTTSEGKKVQKGNVVHLNSLITSGYTKKVDITFIPRRIWSPEATTAELIRKRELRRERFTHEVDFDDFMMPFQKNITEKARALEAALKT

Target ORF information:

RefSeq Version	NM_022096.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ankyrin repeat and EF-hand domain containing 1 (ANKEF1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022096.5

ORF Insert Sequence:

ATGGCTTTAG CAGATAAGAG ACTTGAGAAC TTACAGATCT ACAAAGTTCT TCAATGTGTG 
CGGAACAAAG ACAAGAAGCA GATAGAGAAG CTGACCAAGC TTGGATACCC TGAACTAATC 
AATTATACAG AACCCATTAA TGGACTTAGT GCTTTGCACT TAGCCTCAGT TTCCAATGAT 
ATTGATATGG TCAGCTTTCT CCTTGACCTT GGTGCTCACC CTGATGTGCA AGACCGAATG 
GGCTGTACTC CCACAATGAG GGCTGCAGAA CTGGGCCATG AATTGTCAAT GGAAATATTA 
GCAAAGGCAA AGGCTGATAT GACTATAGTT GATAATGAAG GAAAAGGTGT TTTGTTTTAC 
TGCATTTTAC CGACTAAGCG GCATTATCGC TGTGCTCTGA TCGCCCTTGA ACATGGTGCA 
GATGTCAACA ATTCTACCTA TGAAGGAAAG CCAATATTCC TTAGAGCTTG TGAAGATGCA 
CATGATGTTA AAGATGTGTG CCTGACATTT TTGGAAAAAG GAGCCAATCC TAATGCAATC 
AACTCATCCA CAGGCCGCAC AGCTTTAATG GAAGCGTCAA GAGAAGGGGT AGTGGAAATA 
GTTCGAGGCA TATTGGAAAG AGGAGGTGAA GTGAATGCAT TTGACAACGA CAGGCATCAC 
GCTGCTCATT TTGCTGCTAA AGGAGGCTTT TTCGATATAT TGAAGCTTCT TTTTGCCTAC 
AATGGAGACG TGGGGCTGAT TTCGATAAAT GGGAACACAC CACTTCATTA TGCTGCCATG 
GGTGGTTTTG CAGACTGCTG TAAATATATA GCTCAGCGAG GATGTGACCT GAAATGGAAG 
AATTTAGATC ATAAAACGCC CAGGGCTGTG GCTAAGGAAG GCGGCTTCAA AGCAGCAAGC 
AAAGAAATAC GCCGAGCAGA GAGAATCGCT AATAAACTAG CCAGGCCAGG AGCCAAAAAT 
CCAAATCCAC TGTGGGCCCT TAGACTGCAC GATTGGTCCG TAGAACGTGA GGCTTTCCTC 
CGGGAAGCCT TTGCGGTTTT AGACAGGGGT GATGGAAGCA TCAGCAAGAA CGACTTCGTG 
ATGGTGTTGG AGGAAAGGCA GGATTATGCA AGCTCAGAAC AGCTGGCTGC CATCGCTCAC 
CTTCATGAGA AAACCCGGGG AGGAGGGGTC AATATTAATG AATTCTTTAA AGGAACCAGA 
TATTTAAACA AGTCTTTTGT CTTAGGATCG TATGGACCTA AGAAAAAGGA AAAAGGGATG 
GGCAAAAAAG GAAAGAAAGG GAAATTTGTC TTACCCCTTC CAATCTGTGT CATTCCTGAG 
TACGCGTTTC CACGCCGGCA GGATGGTGGG CCACCGTATT ACATGATTGA GACCTACAAG 
AATGTCACTG ATAGCAGCCG GTTTAATAGA GATCATCCCC CAGAACATCC CATTCAGGAT 
GACTCTGTTT GGTACATTGA TGATTCAGAG AAGGTATTTT CAAACATTAA TATTATCACC 
AAAGCAGGGG ATCTGGCTTC TCTGAAAAAG GCCTTTGAAT CAGGAATACC TGTGGATATG 
AAGGATAATT ATTACAAAAC TCCGCTAATG ACGGCGTGTG CAAGTGGAAA CATAGATGTG 
GTCAAGTTTC TTCTTGAAAA AGGAGCTAAC GTTAATGCAA CAGATAACTT TCTGTGGACT 
CCACTTCATT TTGCATGCCA TGCAGGCCAA CAAGACATTG TTGAGCTTCT TGTTGAATCT 
GGAGCTTTAA TAGATGCAGC TTCAATCAAC AACTCAACTC CTTTAAATAG AGCCATTGAA 
AGCTGCAGAC TGGATACAGT AAAATACCTA CTTGATATTG GTGCTAAATT CCAGCTGGAA 
AATAGAAAAG GGCATAGTGC CATGGACGTT GCAAAGGCAT ATGCTGATTA TAGAATAATT 
GATCTGATTA AAGAAAAGCT AGATAACTTG CCGAAACCAG CAGAAAATCA AAAACTAAAA 
GGCAAGACAC CTCCTATACT GAAGACTGAA GGCCCTGAAA TTAAGAAAGA AGAGGAACTG 
CTGTCATCAA TTTATGGTGT ACCAACCACA TCAGAGGGAA AGAAAGTACA GAAGGGTAAT 
GTGGTTCATC TGAATTCATT GATTACCAGT GGTTATACTA AGAAAGTGGA TATCACATTT 
ATTCCACGGA GGATTTGGAG TCCTGAAGCC ACAACAGCAG AGCTGATCAG GAAGAGGGAA 
CTACGGCGAG AGAGGTTTAC ACATGAGGTG GACTTCGACG ATTTTATGAT GCCTTTTCAG 
AAGAACATCA CAGAGAAAGC TCGAGCACTG GAAGCTGCCT TGAAGACCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Sex-specific linkage to total serum immunoglobulin E in families of children with asthma in Costa Rica.
Human molecular genetics16(3)243-53(2007 Feb)
Raby BA,Soto-Quiros ME,Avila L,Lake SL,Murphy A,Liang C,Fournier E,Spesny M,Sylvia JS,Verner A,Hudson TJ,Klanderman BJ,Freimer NB,Silverman EK,Celed?n JC

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ANKEF1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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