ZNF335 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ZNF335
Entrez Gene ID	63925
Full Name	zinc finger protein 335
Synonyms	MCPH10,NIF-1,NIF1,NIF2
General protein information	Preferred Names zinc finger protein 335 Names zinc finger protein 335 NRC-interacting factor 1 zinc-finger/leucine-zipper co-transducer NIF1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 20 Map Location: 20q13.12
Summary	The protein encoded by this gene enhances transcriptional activation by ligand-bound nuclear hormone receptors. However, it does this not by direct interaction with the receptor, but by direct interaction with the nuclear hormone receptor transcriptional coactivator NRC. The encoded protein may function by altering local chromatin structure. [provided by RefSeq, Jul 2008].
Disorder MIM:	610827
Disorder Html:	?Microcephaly 10, primary, autosomal recessive, 615095 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_022095.3	NP_071378.1	zinc finger protein 335
XM_017028012.1	XP_016883501.1	zinc finger protein 335 isoform X2
XM_011528979.2	XP_011527281.1	zinc finger protein 335 isoform X3
NM_022095.4	NP_071378.1	zinc finger protein 335
XM_005260504.4	XP_005260561.1	zinc finger protein 335 isoform X1
XM_011528979.3	XP_011527281.1	zinc finger protein 335 isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	ZNF335	XP_001160317.1
Canis lupus familiaris (dog)	ZNF335	XP_005635185.1
	ZNF335	XP_001105111.1
Rattus norvegicus (Norway rat)	Zfp335	XP_006235692.1
Danio rerio (zebrafish)	LOC100148891	XP_005167156.1
Homo sapiens (human)	ZNF335	NP_071378.1
Bos taurus (cattle)	ZNF335	NP_001179788.1
Mus musculus (house mouse)	Zfp335	NP_950192.2
Gallus gallus (chicken)	ZNF335	NP_990541.2

Gene Ontology
Bibliography

Related articles in PubMed

Association Between Invisible Basal Ganglia and ZNF335 Mutations: A Case Report.
Sato R, Takanashi J, Tsuyusaki Y, Kato M, Saitsu H, Matsumoto N, Takahashi T
Pediatrics138(3)(2016 Sep)

Anaesthesia and orphan disease: primary autosomal recessive microcephaly-10 caused by a mutation in the ZNF335 gene.
Nishida T, Mihara T, Horiki T, Ka K
European journal of anaesthesiology33(7)543-5(2016 07)

Common polygenic variation in coeliac disease and confirmation of ZNF335 and NIFA as disease susceptibility loci.
Coleman C, Quinn EM, Ryan AW, Conroy J, Trimble V, Mahmud N, Kennedy N, Corvin AP, Morris DW, Donohoe G, O'Morain C, MacMathuna P, Byrnes V, Kiat C, Trynka G, Wijmenga C, Kelleher D, Ennis S, Anney RJ, McManus R
European journal of human genetics : EJHG24(2)291-7(2016 Feb)

IMAC fractionation in combination with LC-MS reveals H2B and NIF-1 peptides as potential bladder cancer biomarkers.
Frantzi M, Zoidakis J, Papadopoulos T, Z?rbig P, Katafigiotis I, Stravodimos K, Lazaris A, Giannopoulou I, Ploumidis A, Mischak H, Mullen W, Vlahou A
Journal of proteome research12(9)3969-79(2013 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

In this article, we describe another family harboring ZNF335 mutations. The mutations were individually transmitted by her parents, indicating that the proband was compound heterozygous for the mutations. We speculate that invisible basal ganglia may be the key feature of ZNF335 mutations.
Title: Association Between Invisible Basal Ganglia and ZNF335 Mutations: A Case Report.

we performed a meta-analysis of our data with the previous reports, identifying two further loci harbouring the ZNF335 and NIFA genes which now exceed genome-wide significance, taking the total number of CD susceptibility loci to 42.
Title: Common polygenic variation in coeliac disease and confirmation of ZNF335 and NIFA as disease susceptibility loci.

NIF-1 expression is associated with tumor grade in bladder cancer.
Title: IMAC fractionation in combination with LC-MS reveals H2B and NIF-1 peptides as potential bladder cancer biomarkers.

Study identifies and characterizes a nuclear zinc finger protein, ZNF335/NIF-1, as a causative gene for severe microcephaly, small somatic size, and neonatal death.
Title: Microcephaly gene links trithorax and REST/NRSF to control neural stem cell proliferation and differentiation.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

The following ZNF335 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ZNF335 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19372	NM_022095.4 Latest version!	Homo sapiens zinc finger protein 335 (ZNF335), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu71155	XM_011528979.3 Latest version!	Homo sapiens zinc finger protein 335 (ZNF335), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu53086	XM_017028012.1 Latest version!	Homo sapiens zinc finger protein 335 (ZNF335), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$643.30 $919.00
OHu53085	XM_005260504.4 Latest version!	Homo sapiens zinc finger protein 335 (ZNF335), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu19372	NM_022095.3	Homo sapiens zinc finger protein 335 (ZNF335), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$519.50-$727.30 $1039.00
OHu71155	XM_011528979.2	Homo sapiens zinc finger protein 335 (ZNF335), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ZNF335 ( NM_022095.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_071378.1 Homo sapiens zinc finger protein 335 (ZNF335), mRNA.

Price & Availability↑

CloneID	OHu19372
Clone ID Related Accession (Same CDS sequence)	NM_022095.3 , NM_022095.4
Accession Version	NM_022095.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-30
Organism	Homo sapiens(human)
Product	zinc finger protein 335
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC041331.2, AF395833.1 and BF755826.1. This sequence is a reference standard in the RefSeqGene project. On Jan 13, 2004 this sequence version replaced NM_022095.2. ##Evidence-Data-START## Transcript exon combination :: AF395833.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGAGA ACGAGGTGGA GAGCAGCAGC GACGCGGCCC CTGGGCCTGG CCGGCCCGAG 
GAGCCCTCTG AGAGCGGCCT GGGTGTGGGC ACCTCAGAAG CCGTGTCCGC CGACAGCAGC 
GACGCCGCGG CCGCCCCGGG GCAGGCAGAG GCCGATGACT CTGGCGTGGG GCAAAGCTCG 
GACCGCGGCA GCCGTTCTCA GGAGGAGGTA TCTGAGAGCA GCTCGAGCGC AGACCCCCTG 
CCTAATAGCT ACCTCCCTGA TTCATCGTCT GTGTCTCATG GGCCAGTGGC AGGGGTGACA 
GGCGGTCCCC CAGCACTTGT GCACTCTAGT GCACTCCCAG ACCCCAACAT GCTGGTGTCC 
GACTGCACAG CTTCCTCCTC GGACCTGGGC TCGGCCATCG ACAAGATCAT CGAGTCCACC 
ATCGGGCCCG ACCTCATCCA GAACTGCATC ACTGTGACCA GTGCTGAGGA TGGCGGGGCC 
GAGACCACAC GGTACCTGAT CCTACAGGGC CCAGATGATG GAGCCCCCAT GACATCACCA 
ATGTCCAGTT CCACCTTGGC CCACAGCCTA GCAGCCATTG AGGCCCTGGC AGATGGCCCC 
ACATCCACAT CCACATGCCT GGAGGCACAG GGTGGGCCCA GCTCCCCGGT GCAGCTGCCC 
CCAGCCTCCG GTGCCGAAGA GCCGGACCTG CAGAGCCTGG AGGCCATGAT GGAGGTGGTG 
GTGGTGCAGC AGTTCAAATG CAAGATGTGC CAGTACCGGA GCAGCACCAA GGCCACACTG 
CTGCGCCACA TGCGGGAACG CCACTTCCGT CCAGTAGCAG CAGCCGCAGC AGCAGCTGGT 
AAAAAAGGAC GTCTACGGAA GTGGAGCACC TCCACCAAGA GCCAAGAGGA AGAGGGACCA 
GAGGAGGAGG ACGATGATGA CATTGTAGAC GCTGGAGCCA TTGATGACCT GGAGGAGGAT 
AGCGACTATA ATCCAGCTGA GGATGAGCCC CGAGGCCGGC AGCTTCGGCT CCAGCGCCCC 
ACCCCCAGTA CCCCAAGGCC CCGAAGGAGA CCTGGCCGGC CCCGGAAGCT GCCCCGCCTG 
GAGATCTCAG ACCTCCCAGA TGGTGTGGAA GGAGAGCCTC TAGTGAGTTC CCAGAGTGGA 
CAGAGCCCTC CAGAGCCACA GGATCCCGAG GCTCCCAGCT CCTCAGGCCC AGGACACCTG 
GTGGCCATGG GCAAGGTGAG CAGGACCCCT GTGGAAGCTG GTGTGAGCCA GTCAGATGCA 
GAGAACGCAG CCCCCTCCTG CCCGGATGAG CATGACACTC TGCCCCGGCG CCGAGGTCGA 
CCTTCCAGGC GCTTCCTAGG CAAGAAATAC CGCAAGTACT ATTACAAGTC GCCCAAACCA 
CTTTTGAGGC CCTTCCTGTG CCGCATCTGT GGTTCTCGCT TTCTGTCCCA CGAGGACCTG 
CGCTTCCACG TCAACTCCCA TGAGGCTGGC GATCCCCAGC TCTTCAAGTG CCTGCAGTGC 
AGCTATCGTT CCCGCCGCTG GTCCTCGCTC AAGGAGCACA TGTTCAACCA CGTGGGCAGC 
AAGCCCTACA AGTGTGACGA GTGCAGCTAC ACCAGTGTCT ACCGGAAGGA CGTCATTCGG 
CACGCCGCTG TGCACAGCCG GGACCGGAAG AAGAGGCCAG ATCCGACTCC AAAGCTGAGC 
TCTTTCCCCT GCCCTGTGTG TGGCCGTGTG TACCCCATGC AGAAAAGACT CACGCAGCAC 
ATGAAGACGC ACAGCACTGA GAAGCCCCAC ATGTGTGACA AGTGTGGAAA GTCCTTTAAG 
AAGCGCTACA CCTTCAAAAT GCACCTGCTC ACGCACATCC AGGCTGTTGC CAACCGCAGG 
TTCAAGTGTG AGTTCTGTGA GTTTGTTTGT GAAGACAAGA AGGCACTGCT GAACCACCAG 
TTGTCCCACG TCAGTGACAA GCCCTTCAAA TGCAGCTTTT GTCCCTACCG CACCTTCCGA 
GAGGACTTCT TGCTGTCCCA TGTGGCTGTC AAGCACACAG GGGCCAAGCC CTTCGCCTGT 
GAGTACTGCC ACTTCAGCAC ACGGCACAAG AAGAACCTGC GCCTGCACGT ACGGTGCCGA 
CACGCAAGCA GCTTCGAGGA ATGGGGGAGG CGCCACCCTG AGGAGCCCCC CTCCCGCCGT 
CGCCCCTTCT TCTCTCTGCA GCAGATTGAG GAGCTGAAGC AGCAGCACAG TGCGGCCCCT 
GGACCACCTC CCAGTTCCCC AGGACCTCCT GAGATACCCC CAGAGGCGAC AACTTTCCAG 
TCATCTGAGG CTCCCTCATT GCTCTGTTCT GACACCCTGG GCGGCGCCAC CATCATCTAC 
CAGCAAGGAG CTGAGGAGTC GACAGCGATG GCCACGCAGA CAGCCTTGGA TCTTCTGCTG 
AACATGAGTG CTCAGCGGGA ACTGGGGGGC ACAGCCCTGC AGGTGGCTGT GGTGAAGTCG 
GAAGATGTGG AAGCAGGGTT AGCATCCCCT GGTGGGCAGC CCTCCCCTGA AGGTGCCACT 
CCACAGGTGG TCACCCTCCA CGTGGCAGAG CCAGGGGGCG GTGCAGCAGC CGAGAGCCAG 
CTAGGCCCTC CTGACCTACC GCAGATCACC CTGGCACCTG GTCCATTTGG TGGGACTGGC 
TACAGTGTCA TCACAGCACC CCCTATGGAG GAGGGAACAT CAGCTCCTGG CACACCTTAC 
AGCGAGGAGC CCGCAGGAGA GGCAGCCCAG GCTGTGGTTG TGAGTGACAC CCTAAAAGAA 
GCTGGCACCC ACTACATCAT GGCTACTGAT GGTACCCAGT TGCACCACAT TGAGCTCACC 
GCAGATGGCT CCATCTCCTT CCCAAGTCCA GATGCTCTGG CCTCTGGTGC CAAATGGCCC 
CTGCTGCAGT GTGGGGGACT GCCCAGAGAC GGCCCTGAGC CCCCATCTCC AGCCAAGACC 
CACTGCGTAG GGGACTCCCA GAGCTCTGCC TCCTCACCTC CTGCAACCAG CAAAGCCCTG 
GGCCTGGCAG TGCCCCCGTC ACCGCCATCT GCAGCCACTG CTGCATCAAA GAAGTTTTCC 
TGCAAGATCT GTGCCGAGGC CTTCCCTGGC CGAGCTGAGA TGGAGAGTCA CAAGCGGGCC 
CACGCTGGGC CTGGTGCCTT CAAGTGCCCC GACTGCCCCT TCAGTGCCCG CCAGTGGCCC 
GAGGTCCGGG CGCACATGGC ACAGCACTCA AGCCTACGGC CCCACCAGTG TAGCCAGTGC 
AGCTTTGCCT CCAAGAACAA GAAGGACCTG CGTCGGCACA TGCTGACTCA CACAAAGGAG 
AAGCCTTTTG CATGCCACCT CTGCGGGCAG CGTTTCAACC GTAACGGGCA CCTCAAGTTC 
CACATCCAGC GGCTGCACAG TCCTGATGGG AGGAAGTCAG GAACCCCTAC AGCCCGGGCC 
CCTACCCAGA CCCCAACCCA GACCATCATC CTGAACAGTG ATGACGAAAC ACTGGCCACC 
CTGCACACTG CACTCCAGTC CAGTCACGGG GTCCTGGGCC CAGAGCGGCT ACAGCAGGCA 
CTGAGCCAGG AACACATCAT CGTTGCCCAG GAACAGACAG TGACCAATCA GGAGGAAGCC 
GCCTACATCC AAGAGATCAC CACGGCAGAT GGCCAGACCG TACAGCACCT GGTGACCTCC 
GACAACCAGG TGCAGTATAT CATCTCCCAG GATGGTGTCC AGCACCTGCT CCCCCAGGAA 
TATGTTGTGG TCCCTGAAGG CCATCACATC CAGGTACAGG AGGGCCAGAT CACACACATC 
CAGTATGAAC AAGGAGCCCC GTTCCTTCAG GAGTCCCAGA TCCAGTATGT GCCTGTGTCC 
CCAGGCCAGC AGCTTGTCAC ACAGGCTCAA CTTGAGGCTG CAGCACACTC AGCTGTCACA 
GCAGTGGCTG ATGCTGCCAT GGCCCAAGCC CAGGGCCTGT TTGGTACAGA CGAGACAGTG 
CCCGAACACA TTCAACAGCT GCAGCACCAG GGCATCGAGT ACGACGTCAT CACCCTGGCC 
GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071378.1
CDS	124..4152
Translation	MEENEVESSSDAAPGPGRPEEPSESGLGVGTSEAVSADSSDAAAAPGQAEADDSGVGQSSDRGSRSQEEVSESSSSADPLPNSYLPDSSSVSHGPVAGVTGGPPALVHSSALPDPNMLVSDCTASSSDLGSAIDKIIESTIGPDLIQNCITVTSAEDGGAETTRYLILQGPDDGAPMTSPMSSSTLAHSLAAIEALADGPTSTSTCLEAQGGPSSPVQLPPASGAEEPDLQSLEAMMEVVVVQQFKCKMCQYRSSTKATLLRHMRERHFRPVAAAAAAAGKKGRLRKWSTSTKSQEEEGPEEEDDDDIVDAGAIDDLEEDSDYNPAEDEPRGRQLRLQRPTPSTPRPRRRPGRPRKLPRLEISDLPDGVEGEPLVSSQSGQSPPEPQDPEAPSSSGPGHLVAMGKVSRTPVEAGVSQSDAENAAPSCPDEHDTLPRRRGRPSRRFLGKKYRKYYYKSPKPLLRPFLCRICGSRFLSHEDLRFHVNSHEAGDPQLFKCLQCSYRSRRWSSLKEHMFNHVGSKPYKCDECSYTSVYRKDVIRHAAVHSRDRKKRPDPTPKLSSFPCPVCGRVYPMQKRLTQHMKTHSTEKPHMCDKCGKSFKKRYTFKMHLLTHIQAVANRRFKCEFCEFVCEDKKALLNHQLSHVSDKPFKCSFCPYRTFREDFLLSHVAVKHTGAKPFACEYCHFSTRHKKNLRLHVRCRHASSFEEWGRRHPEEPPSRRRPFFSLQQIEELKQQHSAAPGPPPSSPGPPEIPPEATTFQSSEAPSLLCSDTLGGATIIYQQGAEESTAMATQTALDLLLNMSAQRELGGTALQVAVVKSEDVEAGLASPGGQPSPEGATPQVVTLHVAEPGGGAAAESQLGPPDLPQITLAPGPFGGTGYSVITAPPMEEGTSAPGTPYSEEPAGEAAQAVVVSDTLKEAGTHYIMATDGTQLHHIELTADGSISFPSPDALASGAKWPLLQCGGLPRDGPEPPSPAKTHCVGDSQSSASSPPATSKALGLAVPPSPPSAATAASKKFSCKICAEAFPGRAEMESHKRAHAGPGAFKCPDCPFSARQWPEVRAHMAQHSSLRPHQCSQCSFASKNKKDLRRHMLTHTKEKPFACHLCGQRFNRNGHLKFHIQRLHSPDGRKSGTPTARAPTQTPTQTIILNSDDETLATLHTALQSSHGVLGPERLQQALSQEHIIVAQEQTVTNQEEAAYIQEITTADGQTVQHLVTSDNQVQYIISQDGVQHLLPQEYVVVPEGHHIQVQEGQITHIQYEQGAPFLQESQIQYVPVSPGQQLVTQAQLEAAAHSAVTAVADAAMAQAQGLFGTDETVPEHIQQLQHQGIEYDVITLADD

Target ORF information:

RefSeq Version	NM_022095.3
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 335 (ZNF335), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022095.3

ORF Insert Sequence:

ATGGAGGAGA ACGAGGTGGA GAGCAGCAGC GACGCGGCCC CTGGGCCTGG CCGGCCCGAG 
GAGCCCTCTG AGAGCGGCCT GGGTGTGGGC ACCTCAGAAG CCGTGTCCGC CGACAGCAGC 
GACGCCGCGG CCGCCCCGGG GCAGGCAGAG GCCGATGACT CTGGCGTGGG GCAAAGCTCG 
GACCGCGGCA GCCGTTCTCA GGAGGAGGTA TCTGAGAGCA GCTCGAGCGC AGACCCCCTG 
CCTAATAGCT ACCTCCCTGA TTCATCGTCT GTGTCTCATG GGCCAGTGGC AGGGGTGACA 
GGCGGTCCCC CAGCACTTGT GCACTCTAGT GCACTCCCAG ACCCCAACAT GCTGGTGTCC 
GACTGCACAG CTTCCTCCTC GGACCTGGGC TCGGCCATCG ACAAGATCAT CGAGTCCACC 
ATCGGGCCCG ACCTCATCCA GAACTGCATC ACTGTGACCA GTGCTGAGGA TGGCGGGGCC 
GAGACCACAC GGTACCTGAT CCTACAGGGC CCAGATGATG GAGCCCCCAT GACATCACCA 
ATGTCCAGTT CCACCTTGGC CCACAGCCTA GCAGCCATTG AGGCCCTGGC AGATGGCCCC 
ACATCCACAT CCACATGCCT GGAGGCACAG GGTGGGCCCA GCTCCCCGGT GCAGCTGCCC 
CCAGCCTCCG GTGCCGAAGA GCCGGACCTG CAGAGCCTGG AGGCCATGAT GGAGGTGGTG 
GTGGTGCAGC AGTTCAAATG CAAGATGTGC CAGTACCGGA GCAGCACCAA GGCCACACTG 
CTGCGCCACA TGCGGGAACG CCACTTCCGT CCAGTAGCAG CAGCCGCAGC AGCAGCTGGT 
AAAAAAGGAC GTCTACGGAA GTGGAGCACC TCCACCAAGA GCCAAGAGGA AGAGGGACCA 
GAGGAGGAGG ACGATGATGA CATTGTAGAC GCTGGAGCCA TTGATGACCT GGAGGAGGAT 
AGCGACTATA ATCCAGCTGA GGATGAGCCC CGAGGCCGGC AGCTTCGGCT CCAGCGCCCC 
ACCCCCAGTA CCCCAAGGCC CCGAAGGAGA CCTGGCCGGC CCCGGAAGCT GCCCCGCCTG 
GAGATCTCAG ACCTCCCAGA TGGTGTGGAA GGAGAGCCTC TAGTGAGTTC CCAGAGTGGA 
CAGAGCCCTC CAGAGCCACA GGATCCCGAG GCTCCCAGCT CCTCAGGCCC AGGACACCTG 
GTGGCCATGG GCAAGGTGAG CAGGACCCCT GTGGAAGCTG GTGTGAGCCA GTCAGATGCA 
GAGAACGCAG CCCCCTCCTG CCCGGATGAG CATGACACTC TGCCCCGGCG CCGAGGTCGA 
CCTTCCAGGC GCTTCCTAGG CAAGAAATAC CGCAAGTACT ATTACAAGTC GCCCAAACCA 
CTTTTGAGGC CCTTCCTGTG CCGCATCTGT GGTTCTCGCT TTCTGTCCCA CGAGGACCTG 
CGCTTCCACG TCAACTCCCA TGAGGCTGGC GATCCCCAGC TCTTCAAGTG CCTGCAGTGC 
AGCTATCGTT CCCGCCGCTG GTCCTCGCTC AAGGAGCACA TGTTCAACCA CGTGGGCAGC 
AAGCCCTACA AGTGTGACGA GTGCAGCTAC ACCAGTGTCT ACCGGAAGGA CGTCATTCGG 
CACGCCGCTG TGCACAGCCG GGACCGGAAG AAGAGGCCAG ATCCGACTCC AAAGCTGAGC 
TCTTTCCCCT GCCCTGTGTG TGGCCGTGTG TACCCCATGC AGAAAAGACT CACGCAGCAC 
ATGAAGACGC ACAGCACTGA GAAGCCCCAC ATGTGTGACA AGTGTGGAAA GTCCTTTAAG 
AAGCGCTACA CCTTCAAAAT GCACCTGCTC ACGCACATCC AGGCTGTTGC CAACCGCAGG 
TTCAAGTGTG AGTTCTGTGA GTTTGTTTGT GAAGACAAGA AGGCACTGCT GAACCACCAG 
TTGTCCCACG TCAGTGACAA GCCCTTCAAA TGCAGCTTTT GTCCCTACCG CACCTTCCGA 
GAGGACTTCT TGCTGTCCCA TGTGGCTGTC AAGCACACAG GGGCCAAGCC CTTCGCCTGT 
GAGTACTGCC ACTTCAGCAC ACGGCACAAG AAGAACCTGC GCCTGCACGT ACGGTGCCGA 
CACGCAAGCA GCTTCGAGGA ATGGGGGAGG CGCCACCCTG AGGAGCCCCC CTCCCGCCGT 
CGCCCCTTCT TCTCTCTGCA GCAGATTGAG GAGCTGAAGC AGCAGCACAG TGCGGCCCCT 
GGACCACCTC CCAGTTCCCC AGGACCTCCT GAGATACCCC CAGAGGCGAC AACTTTCCAG 
TCATCTGAGG CTCCCTCATT GCTCTGTTCT GACACCCTGG GCGGCGCCAC CATCATCTAC 
CAGCAAGGAG CTGAGGAGTC GACAGCGATG GCCACGCAGA CAGCCTTGGA TCTTCTGCTG 
AACATGAGTG CTCAGCGGGA ACTGGGGGGC ACAGCCCTGC AGGTGGCTGT GGTGAAGTCG 
GAAGATGTGG AAGCAGGGTT AGCATCCCCT GGTGGGCAGC CCTCCCCTGA AGGTGCCACT 
CCACAGGTGG TCACCCTCCA CGTGGCAGAG CCAGGGGGCG GTGCAGCAGC CGAGAGCCAG 
CTAGGCCCTC CTGACCTACC GCAGATCACC CTGGCACCTG GTCCATTTGG TGGGACTGGC 
TACAGTGTCA TCACAGCACC CCCTATGGAG GAGGGAACAT CAGCTCCTGG CACACCTTAC 
AGCGAGGAGC CCGCAGGAGA GGCAGCCCAG GCTGTGGTTG TGAGTGACAC CCTAAAAGAA 
GCTGGCACCC ACTACATCAT GGCTACTGAT GGTACCCAGT TGCACCACAT TGAGCTCACC 
GCAGATGGCT CCATCTCCTT CCCAAGTCCA GATGCTCTGG CCTCTGGTGC CAAATGGCCC 
CTGCTGCAGT GTGGGGGACT GCCCAGAGAC GGCCCTGAGC CCCCATCTCC AGCCAAGACC 
CACTGCGTAG GGGACTCCCA GAGCTCTGCC TCCTCACCTC CTGCAACCAG CAAAGCCCTG 
GGCCTGGCAG TGCCCCCGTC ACCGCCATCT GCAGCCACTG CTGCATCAAA GAAGTTTTCC 
TGCAAGATCT GTGCCGAGGC CTTCCCTGGC CGAGCTGAGA TGGAGAGTCA CAAGCGGGCC 
CACGCTGGGC CTGGTGCCTT CAAGTGCCCC GACTGCCCCT TCAGTGCCCG CCAGTGGCCC 
GAGGTCCGGG CGCACATGGC ACAGCACTCA AGCCTACGGC CCCACCAGTG TAGCCAGTGC 
AGCTTTGCCT CCAAGAACAA GAAGGACCTG CGTCGGCACA TGCTGACTCA CACAAAGGAG 
AAGCCTTTTG CATGCCACCT CTGCGGGCAG CGTTTCAACC GTAACGGGCA CCTCAAGTTC 
CACATCCAGC GGCTGCACAG TCCTGATGGG AGGAAGTCAG GAACCCCTAC AGCCCGGGCC 
CCTACCCAGA CCCCAACCCA GACCATCATC CTGAACAGTG ATGACGAAAC ACTGGCCACC 
CTGCACACTG CACTCCAGTC CAGTCACGGG GTCCTGGGCC CAGAGCGGCT ACAGCAGGCA 
CTGAGCCAGG AACACATCAT CGTTGCCCAG GAACAGACAG TGACCAATCA GGAGGAAGCC 
GCCTACATCC AAGAGATCAC CACGGCAGAT GGCCAGACCG TACAGCACCT GGTGACCTCC 
GACAACCAGG TGCAGTATAT CATCTCCCAG GATGGTGTCC AGCACCTGCT CCCCCAGGAA 
TATGTTGTGG TCCCTGAAGG CCATCACATC CAGGTACAGG AGGGCCAGAT CACACACATC 
CAGTATGAAC AAGGAGCCCC GTTCCTTCAG GAGTCCCAGA TCCAGTATGT GCCTGTGTCC 
CCAGGCCAGC AGCTTGTCAC ACAGGCTCAA CTTGAGGCTG CAGCACACTC AGCTGTCACA 
GCAGTGGCTG ATGCTGCCAT GGCCCAAGCC CAGGGCCTGT TTGGTACAGA CGAGACAGTG 
CCCGAACACA TTCAACAGCT GCAGCACCAG GGCATCGAGT ACGACGTCAT CACCCTGGCC 
GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF335 ( NM_022095.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_071378.1 Homo sapiens zinc finger protein 335 (ZNF335), mRNA.

Price & Availability↑

CloneID	OHu19372
Clone ID Related Accession (Same CDS sequence)	NM_022095.3 , NM_022095.4
Accession Version	NM_022095.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4029bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-18
Organism	Homo sapiens(human)
Product	zinc finger protein 335
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL162458.10, BC041331.2, AF395833.1 and BF755826.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_022095.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF395833.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000322927.3/ ENSP00000325326.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAGGAGA ACGAGGTGGA GAGCAGCAGC GACGCGGCCC CTGGGCCTGG CCGGCCCGAG 
GAGCCCTCTG AGAGCGGCCT GGGTGTGGGC ACCTCAGAAG CCGTGTCCGC CGACAGCAGC 
GACGCCGCGG CCGCCCCGGG GCAGGCAGAG GCCGATGACT CTGGCGTGGG GCAAAGCTCG 
GACCGCGGCA GCCGTTCTCA GGAGGAGGTA TCTGAGAGCA GCTCGAGCGC AGACCCCCTG 
CCTAATAGCT ACCTCCCTGA TTCATCGTCT GTGTCTCATG GGCCAGTGGC AGGGGTGACA 
GGCGGTCCCC CAGCACTTGT GCACTCTAGT GCACTCCCAG ACCCCAACAT GCTGGTGTCC 
GACTGCACAG CTTCCTCCTC GGACCTGGGC TCGGCCATCG ACAAGATCAT CGAGTCCACC 
ATCGGGCCCG ACCTCATCCA GAACTGCATC ACTGTGACCA GTGCTGAGGA TGGCGGGGCC 
GAGACCACAC GGTACCTGAT CCTACAGGGC CCAGATGATG GAGCCCCCAT GACATCACCA 
ATGTCCAGTT CCACCTTGGC CCACAGCCTA GCAGCCATTG AGGCCCTGGC AGATGGCCCC 
ACATCCACAT CCACATGCCT GGAGGCACAG GGTGGGCCCA GCTCCCCGGT GCAGCTGCCC 
CCAGCCTCCG GTGCCGAAGA GCCGGACCTG CAGAGCCTGG AGGCCATGAT GGAGGTGGTG 
GTGGTGCAGC AGTTCAAATG CAAGATGTGC CAGTACCGGA GCAGCACCAA GGCCACACTG 
CTGCGCCACA TGCGGGAACG CCACTTCCGT CCAGTAGCAG CAGCCGCAGC AGCAGCTGGT 
AAAAAAGGAC GTCTACGGAA GTGGAGCACC TCCACCAAGA GCCAAGAGGA AGAGGGACCA 
GAGGAGGAGG ACGATGATGA CATTGTAGAC GCTGGAGCCA TTGATGACCT GGAGGAGGAT 
AGCGACTATA ATCCAGCTGA GGATGAGCCC CGAGGCCGGC AGCTTCGGCT CCAGCGCCCC 
ACCCCCAGTA CCCCAAGGCC CCGAAGGAGA CCTGGCCGGC CCCGGAAGCT GCCCCGCCTG 
GAGATCTCAG ACCTCCCAGA TGGTGTGGAA GGAGAGCCTC TAGTGAGTTC CCAGAGTGGA 
CAGAGCCCTC CAGAGCCACA GGATCCCGAG GCTCCCAGCT CCTCAGGCCC AGGACACCTG 
GTGGCCATGG GCAAGGTGAG CAGGACCCCT GTGGAAGCTG GTGTGAGCCA GTCAGATGCA 
GAGAACGCAG CCCCCTCCTG CCCGGATGAG CATGACACTC TGCCCCGGCG CCGAGGTCGA 
CCTTCCAGGC GCTTCCTAGG CAAGAAATAC CGCAAGTACT ATTACAAGTC GCCCAAACCA 
CTTTTGAGGC CCTTCCTGTG CCGCATCTGT GGTTCTCGCT TTCTGTCCCA CGAGGACCTG 
CGCTTCCACG TCAACTCCCA TGAGGCTGGC GATCCCCAGC TCTTCAAGTG CCTGCAGTGC 
AGCTATCGTT CCCGCCGCTG GTCCTCGCTC AAGGAGCACA TGTTCAACCA CGTGGGCAGC 
AAGCCCTACA AGTGTGACGA GTGCAGCTAC ACCAGTGTCT ACCGGAAGGA CGTCATTCGG 
CACGCCGCTG TGCACAGCCG GGACCGGAAG AAGAGGCCAG ATCCGACTCC AAAGCTGAGC 
TCTTTCCCCT GCCCTGTGTG TGGCCGTGTG TACCCCATGC AGAAAAGACT CACGCAGCAC 
ATGAAGACGC ACAGCACTGA GAAGCCCCAC ATGTGTGACA AGTGTGGAAA GTCCTTTAAG 
AAGCGCTACA CCTTCAAAAT GCACCTGCTC ACGCACATCC AGGCTGTTGC CAACCGCAGG 
TTCAAGTGTG AGTTCTGTGA GTTTGTTTGT GAAGACAAGA AGGCACTGCT GAACCACCAG 
TTGTCCCACG TCAGTGACAA GCCCTTCAAA TGCAGCTTTT GTCCCTACCG CACCTTCCGA 
GAGGACTTCT TGCTGTCCCA TGTGGCTGTC AAGCACACAG GGGCCAAGCC CTTCGCCTGT 
GAGTACTGCC ACTTCAGCAC ACGGCACAAG AAGAACCTGC GCCTGCACGT ACGGTGCCGA 
CACGCAAGCA GCTTCGAGGA ATGGGGGAGG CGCCACCCTG AGGAGCCCCC CTCCCGCCGT 
CGCCCCTTCT TCTCTCTGCA GCAGATTGAG GAGCTGAAGC AGCAGCACAG TGCGGCCCCT 
GGACCACCTC CCAGTTCCCC AGGACCTCCT GAGATACCCC CAGAGGCGAC AACTTTCCAG 
TCATCTGAGG CTCCCTCATT GCTCTGTTCT GACACCCTGG GCGGCGCCAC CATCATCTAC 
CAGCAAGGAG CTGAGGAGTC GACAGCGATG GCCACGCAGA CAGCCTTGGA TCTTCTGCTG 
AACATGAGTG CTCAGCGGGA ACTGGGGGGC ACAGCCCTGC AGGTGGCTGT GGTGAAGTCG 
GAAGATGTGG AAGCAGGGTT AGCATCCCCT GGTGGGCAGC CCTCCCCTGA AGGTGCCACT 
CCACAGGTGG TCACCCTCCA CGTGGCAGAG CCAGGGGGCG GTGCAGCAGC CGAGAGCCAG 
CTAGGCCCTC CTGACCTACC GCAGATCACC CTGGCACCTG GTCCATTTGG TGGGACTGGC 
TACAGTGTCA TCACAGCACC CCCTATGGAG GAGGGAACAT CAGCTCCTGG CACACCTTAC 
AGCGAGGAGC CCGCAGGAGA GGCAGCCCAG GCTGTGGTTG TGAGTGACAC CCTAAAAGAA 
GCTGGCACCC ACTACATCAT GGCTACTGAT GGTACCCAGT TGCACCACAT TGAGCTCACC 
GCAGATGGCT CCATCTCCTT CCCAAGTCCA GATGCTCTGG CCTCTGGTGC CAAATGGCCC 
CTGCTGCAGT GTGGGGGACT GCCCAGAGAC GGCCCTGAGC CCCCATCTCC AGCCAAGACC 
CACTGCGTAG GGGACTCCCA GAGCTCTGCC TCCTCACCTC CTGCAACCAG CAAAGCCCTG 
GGCCTGGCAG TGCCCCCGTC ACCGCCATCT GCAGCCACTG CTGCATCAAA GAAGTTTTCC 
TGCAAGATCT GTGCCGAGGC CTTCCCTGGC CGAGCTGAGA TGGAGAGTCA CAAGCGGGCC 
CACGCTGGGC CTGGTGCCTT CAAGTGCCCC GACTGCCCCT TCAGTGCCCG CCAGTGGCCC 
GAGGTCCGGG CGCACATGGC ACAGCACTCA AGCCTACGGC CCCACCAGTG TAGCCAGTGC 
AGCTTTGCCT CCAAGAACAA GAAGGACCTG CGTCGGCACA TGCTGACTCA CACAAAGGAG 
AAGCCTTTTG CATGCCACCT CTGCGGGCAG CGTTTCAACC GTAACGGGCA CCTCAAGTTC 
CACATCCAGC GGCTGCACAG TCCTGATGGG AGGAAGTCAG GAACCCCTAC AGCCCGGGCC 
CCTACCCAGA CCCCAACCCA GACCATCATC CTGAACAGTG ATGACGAAAC ACTGGCCACC 
CTGCACACTG CACTCCAGTC CAGTCACGGG GTCCTGGGCC CAGAGCGGCT ACAGCAGGCA 
CTGAGCCAGG AACACATCAT CGTTGCCCAG GAACAGACAG TGACCAATCA GGAGGAAGCC 
GCCTACATCC AAGAGATCAC CACGGCAGAT GGCCAGACCG TACAGCACCT GGTGACCTCC 
GACAACCAGG TGCAGTATAT CATCTCCCAG GATGGTGTCC AGCACCTGCT CCCCCAGGAA 
TATGTTGTGG TCCCTGAAGG CCATCACATC CAGGTACAGG AGGGCCAGAT CACACACATC 
CAGTATGAAC AAGGAGCCCC GTTCCTTCAG GAGTCCCAGA TCCAGTATGT GCCTGTGTCC 
CCAGGCCAGC AGCTTGTCAC ACAGGCTCAA CTTGAGGCTG CAGCACACTC AGCTGTCACA 
GCAGTGGCTG ATGCTGCCAT GGCCCAAGCC CAGGGCCTGT TTGGTACAGA CGAGACAGTG 
CCCGAACACA TTCAACAGCT GCAGCACCAG GGCATCGAGT ACGACGTCAT CACCCTGGCC 
GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_071378.1
CDS	133..4161
Translation	MEENEVESSSDAAPGPGRPEEPSESGLGVGTSEAVSADSSDAAAAPGQAEADDSGVGQSSDRGSRSQEEVSESSSSADPLPNSYLPDSSSVSHGPVAGVTGGPPALVHSSALPDPNMLVSDCTASSSDLGSAIDKIIESTIGPDLIQNCITVTSAEDGGAETTRYLILQGPDDGAPMTSPMSSSTLAHSLAAIEALADGPTSTSTCLEAQGGPSSPVQLPPASGAEEPDLQSLEAMMEVVVVQQFKCKMCQYRSSTKATLLRHMRERHFRPVAAAAAAAGKKGRLRKWSTSTKSQEEEGPEEEDDDDIVDAGAIDDLEEDSDYNPAEDEPRGRQLRLQRPTPSTPRPRRRPGRPRKLPRLEISDLPDGVEGEPLVSSQSGQSPPEPQDPEAPSSSGPGHLVAMGKVSRTPVEAGVSQSDAENAAPSCPDEHDTLPRRRGRPSRRFLGKKYRKYYYKSPKPLLRPFLCRICGSRFLSHEDLRFHVNSHEAGDPQLFKCLQCSYRSRRWSSLKEHMFNHVGSKPYKCDECSYTSVYRKDVIRHAAVHSRDRKKRPDPTPKLSSFPCPVCGRVYPMQKRLTQHMKTHSTEKPHMCDKCGKSFKKRYTFKMHLLTHIQAVANRRFKCEFCEFVCEDKKALLNHQLSHVSDKPFKCSFCPYRTFREDFLLSHVAVKHTGAKPFACEYCHFSTRHKKNLRLHVRCRHASSFEEWGRRHPEEPPSRRRPFFSLQQIEELKQQHSAAPGPPPSSPGPPEIPPEATTFQSSEAPSLLCSDTLGGATIIYQQGAEESTAMATQTALDLLLNMSAQRELGGTALQVAVVKSEDVEAGLASPGGQPSPEGATPQVVTLHVAEPGGGAAAESQLGPPDLPQITLAPGPFGGTGYSVITAPPMEEGTSAPGTPYSEEPAGEAAQAVVVSDTLKEAGTHYIMATDGTQLHHIELTADGSISFPSPDALASGAKWPLLQCGGLPRDGPEPPSPAKTHCVGDSQSSASSPPATSKALGLAVPPSPPSAATAASKKFSCKICAEAFPGRAEMESHKRAHAGPGAFKCPDCPFSARQWPEVRAHMAQHSSLRPHQCSQCSFASKNKKDLRRHMLTHTKEKPFACHLCGQRFNRNGHLKFHIQRLHSPDGRKSGTPTARAPTQTPTQTIILNSDDETLATLHTALQSSHGVLGPERLQQALSQEHIIVAQEQTVTNQEEAAYIQEITTADGQTVQHLVTSDNQVQYIISQDGVQHLLPQEYVVVPEGHHIQVQEGQITHIQYEQGAPFLQESQIQYVPVSPGQQLVTQAQLEAAAHSAVTAVADAAMAQAQGLFGTDETVPEHIQQLQHQGIEYDVITLADD

Target ORF information:

RefSeq Version	NM_022095.4
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 335 (ZNF335), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022095.4

ORF Insert Sequence:

ATGGAGGAGA ACGAGGTGGA GAGCAGCAGC GACGCGGCCC CTGGGCCTGG CCGGCCCGAG 
GAGCCCTCTG AGAGCGGCCT GGGTGTGGGC ACCTCAGAAG CCGTGTCCGC CGACAGCAGC 
GACGCCGCGG CCGCCCCGGG GCAGGCAGAG GCCGATGACT CTGGCGTGGG GCAAAGCTCG 
GACCGCGGCA GCCGTTCTCA GGAGGAGGTA TCTGAGAGCA GCTCGAGCGC AGACCCCCTG 
CCTAATAGCT ACCTCCCTGA TTCATCGTCT GTGTCTCATG GGCCAGTGGC AGGGGTGACA 
GGCGGTCCCC CAGCACTTGT GCACTCTAGT GCACTCCCAG ACCCCAACAT GCTGGTGTCC 
GACTGCACAG CTTCCTCCTC GGACCTGGGC TCGGCCATCG ACAAGATCAT CGAGTCCACC 
ATCGGGCCCG ACCTCATCCA GAACTGCATC ACTGTGACCA GTGCTGAGGA TGGCGGGGCC 
GAGACCACAC GGTACCTGAT CCTACAGGGC CCAGATGATG GAGCCCCCAT GACATCACCA 
ATGTCCAGTT CCACCTTGGC CCACAGCCTA GCAGCCATTG AGGCCCTGGC AGATGGCCCC 
ACATCCACAT CCACATGCCT GGAGGCACAG GGTGGGCCCA GCTCCCCGGT GCAGCTGCCC 
CCAGCCTCCG GTGCCGAAGA GCCGGACCTG CAGAGCCTGG AGGCCATGAT GGAGGTGGTG 
GTGGTGCAGC AGTTCAAATG CAAGATGTGC CAGTACCGGA GCAGCACCAA GGCCACACTG 
CTGCGCCACA TGCGGGAACG CCACTTCCGT CCAGTAGCAG CAGCCGCAGC AGCAGCTGGT 
AAAAAAGGAC GTCTACGGAA GTGGAGCACC TCCACCAAGA GCCAAGAGGA AGAGGGACCA 
GAGGAGGAGG ACGATGATGA CATTGTAGAC GCTGGAGCCA TTGATGACCT GGAGGAGGAT 
AGCGACTATA ATCCAGCTGA GGATGAGCCC CGAGGCCGGC AGCTTCGGCT CCAGCGCCCC 
ACCCCCAGTA CCCCAAGGCC CCGAAGGAGA CCTGGCCGGC CCCGGAAGCT GCCCCGCCTG 
GAGATCTCAG ACCTCCCAGA TGGTGTGGAA GGAGAGCCTC TAGTGAGTTC CCAGAGTGGA 
CAGAGCCCTC CAGAGCCACA GGATCCCGAG GCTCCCAGCT CCTCAGGCCC AGGACACCTG 
GTGGCCATGG GCAAGGTGAG CAGGACCCCT GTGGAAGCTG GTGTGAGCCA GTCAGATGCA 
GAGAACGCAG CCCCCTCCTG CCCGGATGAG CATGACACTC TGCCCCGGCG CCGAGGTCGA 
CCTTCCAGGC GCTTCCTAGG CAAGAAATAC CGCAAGTACT ATTACAAGTC GCCCAAACCA 
CTTTTGAGGC CCTTCCTGTG CCGCATCTGT GGTTCTCGCT TTCTGTCCCA CGAGGACCTG 
CGCTTCCACG TCAACTCCCA TGAGGCTGGC GATCCCCAGC TCTTCAAGTG CCTGCAGTGC 
AGCTATCGTT CCCGCCGCTG GTCCTCGCTC AAGGAGCACA TGTTCAACCA CGTGGGCAGC 
AAGCCCTACA AGTGTGACGA GTGCAGCTAC ACCAGTGTCT ACCGGAAGGA CGTCATTCGG 
CACGCCGCTG TGCACAGCCG GGACCGGAAG AAGAGGCCAG ATCCGACTCC AAAGCTGAGC 
TCTTTCCCCT GCCCTGTGTG TGGCCGTGTG TACCCCATGC AGAAAAGACT CACGCAGCAC 
ATGAAGACGC ACAGCACTGA GAAGCCCCAC ATGTGTGACA AGTGTGGAAA GTCCTTTAAG 
AAGCGCTACA CCTTCAAAAT GCACCTGCTC ACGCACATCC AGGCTGTTGC CAACCGCAGG 
TTCAAGTGTG AGTTCTGTGA GTTTGTTTGT GAAGACAAGA AGGCACTGCT GAACCACCAG 
TTGTCCCACG TCAGTGACAA GCCCTTCAAA TGCAGCTTTT GTCCCTACCG CACCTTCCGA 
GAGGACTTCT TGCTGTCCCA TGTGGCTGTC AAGCACACAG GGGCCAAGCC CTTCGCCTGT 
GAGTACTGCC ACTTCAGCAC ACGGCACAAG AAGAACCTGC GCCTGCACGT ACGGTGCCGA 
CACGCAAGCA GCTTCGAGGA ATGGGGGAGG CGCCACCCTG AGGAGCCCCC CTCCCGCCGT 
CGCCCCTTCT TCTCTCTGCA GCAGATTGAG GAGCTGAAGC AGCAGCACAG TGCGGCCCCT 
GGACCACCTC CCAGTTCCCC AGGACCTCCT GAGATACCCC CAGAGGCGAC AACTTTCCAG 
TCATCTGAGG CTCCCTCATT GCTCTGTTCT GACACCCTGG GCGGCGCCAC CATCATCTAC 
CAGCAAGGAG CTGAGGAGTC GACAGCGATG GCCACGCAGA CAGCCTTGGA TCTTCTGCTG 
AACATGAGTG CTCAGCGGGA ACTGGGGGGC ACAGCCCTGC AGGTGGCTGT GGTGAAGTCG 
GAAGATGTGG AAGCAGGGTT AGCATCCCCT GGTGGGCAGC CCTCCCCTGA AGGTGCCACT 
CCACAGGTGG TCACCCTCCA CGTGGCAGAG CCAGGGGGCG GTGCAGCAGC CGAGAGCCAG 
CTAGGCCCTC CTGACCTACC GCAGATCACC CTGGCACCTG GTCCATTTGG TGGGACTGGC 
TACAGTGTCA TCACAGCACC CCCTATGGAG GAGGGAACAT CAGCTCCTGG CACACCTTAC 
AGCGAGGAGC CCGCAGGAGA GGCAGCCCAG GCTGTGGTTG TGAGTGACAC CCTAAAAGAA 
GCTGGCACCC ACTACATCAT GGCTACTGAT GGTACCCAGT TGCACCACAT TGAGCTCACC 
GCAGATGGCT CCATCTCCTT CCCAAGTCCA GATGCTCTGG CCTCTGGTGC CAAATGGCCC 
CTGCTGCAGT GTGGGGGACT GCCCAGAGAC GGCCCTGAGC CCCCATCTCC AGCCAAGACC 
CACTGCGTAG GGGACTCCCA GAGCTCTGCC TCCTCACCTC CTGCAACCAG CAAAGCCCTG 
GGCCTGGCAG TGCCCCCGTC ACCGCCATCT GCAGCCACTG CTGCATCAAA GAAGTTTTCC 
TGCAAGATCT GTGCCGAGGC CTTCCCTGGC CGAGCTGAGA TGGAGAGTCA CAAGCGGGCC 
CACGCTGGGC CTGGTGCCTT CAAGTGCCCC GACTGCCCCT TCAGTGCCCG CCAGTGGCCC 
GAGGTCCGGG CGCACATGGC ACAGCACTCA AGCCTACGGC CCCACCAGTG TAGCCAGTGC 
AGCTTTGCCT CCAAGAACAA GAAGGACCTG CGTCGGCACA TGCTGACTCA CACAAAGGAG 
AAGCCTTTTG CATGCCACCT CTGCGGGCAG CGTTTCAACC GTAACGGGCA CCTCAAGTTC 
CACATCCAGC GGCTGCACAG TCCTGATGGG AGGAAGTCAG GAACCCCTAC AGCCCGGGCC 
CCTACCCAGA CCCCAACCCA GACCATCATC CTGAACAGTG ATGACGAAAC ACTGGCCACC 
CTGCACACTG CACTCCAGTC CAGTCACGGG GTCCTGGGCC CAGAGCGGCT ACAGCAGGCA 
CTGAGCCAGG AACACATCAT CGTTGCCCAG GAACAGACAG TGACCAATCA GGAGGAAGCC 
GCCTACATCC AAGAGATCAC CACGGCAGAT GGCCAGACCG TACAGCACCT GGTGACCTCC 
GACAACCAGG TGCAGTATAT CATCTCCCAG GATGGTGTCC AGCACCTGCT CCCCCAGGAA 
TATGTTGTGG TCCCTGAAGG CCATCACATC CAGGTACAGG AGGGCCAGAT CACACACATC 
CAGTATGAAC AAGGAGCCCC GTTCCTTCAG GAGTCCCAGA TCCAGTATGT GCCTGTGTCC 
CCAGGCCAGC AGCTTGTCAC ACAGGCTCAA CTTGAGGCTG CAGCACACTC AGCTGTCACA 
GCAGTGGCTG ATGCTGCCAT GGCCCAAGCC CAGGGCCTGT TTGGTACAGA CGAGACAGTG 
CCCGAACACA TTCAACAGCT GCAGCACCAG GGCATCGAGT ACGACGTCAT CACCCTGGCC 
GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF335 ( XM_011528979.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527281.1 Homo sapiens zinc finger protein 335 (ZNF335), transcript variant X4, mRNA.

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CloneID	OHu71155
Clone ID Related Accession (Same CDS sequence)	XM_011528979.2 , XM_011528979.3
Accession Version	XM_011528979.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	zinc finger protein 335 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011528979.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACGAGGTGGA GAGCAGCAGC GACGCGGCCC CTGGGCCTGG CCGGCCCGAG 
GAGCCCTCTG AGAGCGGCCT GGGTGTGGGC ACCTCAGAAG CCGTGTCCGC CGACAGCAGC 
GACGCCGCGG CCGCCCCGGG GCAGGCAGAG GCCGATGACT CTGGCGTGGG GCAAAGCTCG 
GACCGCGGCA GCCGTTCTCA GGAGGAGGTA TCTGAGAGCA GCTCGAGCGC AGACCCCCTG 
CCTAATAGCT ACCTCCCTGA TTCATCGTCT GTGTCTCATG GGCCAGTGGC AGGGGTGACA 
GGCGGTCCCC CAGCACTTGT GCACTCTAGT GCACTCCCAG ACCCCAACAT GCTGGTGTCC 
GACTGCACAG CTTCCTCCTC GGACCTGGGC TCGGCCATCG ACAAGATCAT CGAGTCCACC 
ATCGGGCCCG ACCTCATCCA GAACTGCATC ACTGTGACCA GTGCTGAGGA TGGCGGGGCC 
GAGACCACAC GGTACCTGAT CCTACAGGGC CCAGATGATG GAGCCCCCAT GACATCACCA 
ATGTCCAGTT CCACCTTGGC CCACAGCCTA GCAGCCATTG AGGCCCTGGC AGATGGCCCC 
ACATCCACAT CCACATGCCT GGAGGCACAG GGTGGGCCCA GCTCCCCGGT GCAGCTGCCC 
CCAGCCTCCG GTGCCGAAGA GCCGGACCTG CAGAGCCTGG AGGCCATGAT GGAGGTGGTG 
GTGGTGCAGC AGTTCAAATG CAAGATGTGC CAGTACCGGA GCAGCACCAA GGCCACACTG 
CTGCGCCACA TGCGGGAACG CCACTTCCGT CCAGTAGCAG CAGCCGCAGC AGCAGCTGGT 
AAAAAAGGAC GTCTACGGAA GTGGAGCACC TCCACCAAGA GCCAAGAGGA AGAGGGACCA 
GAGGAGGAGG ACGATGATGA CATTGTAGAC GCTGGAGCCA TTGATGACCT GGAGGAGGAT 
AGCGACTATA ATCCAGCTGA GGATGAGCCC CGAGGCCGGC AGCTTCGGCT CCAGCGCCCC 
ACCCCCAGTA CCCCAAGGCC CCGAAGGAGA CCTGGCCGGC CCCGGAAGCT GCCCCGCCTG 
GAGATCTCAG ACCTCCCAGA TGGTGTGGAA GGAGAGCCTC TAGTGAGTTC CCAGAGTGGA 
CAGAGCCCTC CAGAGCCACA GGATCCCGAG GCTCCCAGCT CCTCAGGCCC AGGACACCTG 
GTGGCCATGG GCAAGGTGAG CAGGACCCCT GTGGAAGCTG GTGTGAGCCA GTCAGATGCA 
GAGAACGCAG CCCCCTCCTG CCCGGATGAG CATGACACTC TGCCCCGGCG CCGAGGTCGA 
CCTTCCAGGC GCTTCCTAGG CAAGAAATAC CGCAAGTACT ATTACAAGTC GCCCAAACCA 
CTTTTGAGGC CCTTCCTGTG CCGCATCTGT GGTTCTCGCT TTCTGTCCCA CGAGGACCTG 
CGCTTCCACG TCAACTCCCA TGAGGCTGGC GATCCCCAGC TCTTCAAGTG CCTGCAGTGC 
AGCTATCGTT CCCGCCGCTG GTCCTCGCTC AAGGAGCACA TGTTCAACCA CGTGGGCAGC 
AAGCCCTACA AGTGTGACGA GTGCAGCTAC ACCAGTGTCT ACCGGAAGGA CGTCATTCGG 
CACGCCGCTG TGCACAGCCG GGACCGGAAG AAGAGGCCAG ATCCGACTCC AAAGCTGAGC 
TCTTTCCCCT GCCCTGTGTG TGGCCGTGTG TACCCCATGC AGAAAAGACT CACGCAGCAC 
ATGAAGACGC ACAGCACTGA GAAGCCCCAC ATGTGTGACA AGTGTGGAAA GTCCTTTAAG 
AAGCGCTACA CCTTCAAAAT GCACCTGCTC ACGCACATCC AGGCTGTTGC CAACCGCAGG 
TTCAAGTGTG AGTTCTGTGA GTTTGTTTGT GAAGACAAGA AGGCACTGCT GAACCACCAG 
TTGTCCCACG TCAGTGACAA GCCCTTCAAA TGCAGCTTTT GTCCCTACCG CACCTTCCGA 
GAGGACTTCT TGCTGTCCCA TGTGGCTGTC AAGCACACAG GGGCCAAGCC CTTCGCCTGT 
GAGTACTGCC ACTTCAGCAC ACGGCACAAG AAGAACCTGC GCCTGCACGT ACGGTGCCGA 
CACGCAAGCA GCTTCGAGGA ATGGGGGAGG CGCCACCCTG AGGAGCCCCC CTCCCGCCGT 
CGCCCCTTCT TCTCTCTGCA GCAGATTGAG GAGCTGAAGC AGCAGCACAG TGCGGCCCCT 
GGACCACCTC CCAGTTCCCC AGGACCTCCT GAGATACCCC CAGAGGCGAC AACTTTCCAG 
TCATCTGAGG CTCCCTCATT GCTCTGTTCT GACACCCTGG GCGGCGCCAC CATCATCTAC 
CAGCAAGGAG CTGAGGAGTC GACAGCGATG GCCACGCAGA CAGCCTTGGA TCTTCTGCTG 
AACATGAGTG CTCAGCGGGA ACTGGGGGGC ACAGCCCTGC AGGTGGCTGT GGTGAAGTCG 
GAAGATGTGG AAGCAGGGTT AGCATCCCCT GGTGGGCAGC CCTCCCCTGA AGGTGCCACT 
CCACAGGTGG TCACCCTCCA CGTGGCAGAG CCAGGGGGCG GTGCAGCAGC CGAGAGCCAG 
CTAGGCCCTC CTGACCTACC GCAGATCACC CTGGCACCTG GTCCATTTGG TGGGACTGGC 
TACAGTGTCA TCACAGCACC CCCTATGGAG GAGGGAACAT CAGCTCCTGG CACACCTTAC 
AGGGGGCTCC TGTGGCCCTC CATCTTCTCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527281.1
CDS	492..3224
Translation	MEENEVESSSDAAPGPGRPEEPSESGLGVGTSEAVSADSSDAAAAPGQAEADDSGVGQSSDRGSRSQEEVSESSSSADPLPNSYLPDSSSVSHGPVAGVTGGPPALVHSSALPDPNMLVSDCTASSSDLGSAIDKIIESTIGPDLIQNCITVTSAEDGGAETTRYLILQGPDDGAPMTSPMSSSTLAHSLAAIEALADGPTSTSTCLEAQGGPSSPVQLPPASGAEEPDLQSLEAMMEVVVVQQFKCKMCQYRSSTKATLLRHMRERHFRPVAAAAAAAGKKGRLRKWSTSTKSQEEEGPEEEDDDDIVDAGAIDDLEEDSDYNPAEDEPRGRQLRLQRPTPSTPRPRRRPGRPRKLPRLEISDLPDGVEGEPLVSSQSGQSPPEPQDPEAPSSSGPGHLVAMGKVSRTPVEAGVSQSDAENAAPSCPDEHDTLPRRRGRPSRRFLGKKYRKYYYKSPKPLLRPFLCRICGSRFLSHEDLRFHVNSHEAGDPQLFKCLQCSYRSRRWSSLKEHMFNHVGSKPYKCDECSYTSVYRKDVIRHAAVHSRDRKKRPDPTPKLSSFPCPVCGRVYPMQKRLTQHMKTHSTEKPHMCDKCGKSFKKRYTFKMHLLTHIQAVANRRFKCEFCEFVCEDKKALLNHQLSHVSDKPFKCSFCPYRTFREDFLLSHVAVKHTGAKPFACEYCHFSTRHKKNLRLHVRCRHASSFEEWGRRHPEEPPSRRRPFFSLQQIEELKQQHSAAPGPPPSSPGPPEIPPEATTFQSSEAPSLLCSDTLGGATIIYQQGAEESTAMATQTALDLLLNMSAQRELGGTALQVAVVKSEDVEAGLASPGGQPSPEGATPQVVTLHVAEPGGGAAAESQLGPPDLPQITLAPGPFGGTGYSVITAPPMEEGTSAPGTPYRGLLWPSIFS

Target ORF information:

RefSeq Version	XM_011528979.2
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 335 (ZNF335), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528979.2

ORF Insert Sequence:

ATGGAGGAGA ACGAGGTGGA GAGCAGCAGC GACGCGGCCC CTGGGCCTGG CCGGCCCGAG 
GAGCCCTCTG AGAGCGGCCT GGGTGTGGGC ACCTCAGAAG CCGTGTCCGC CGACAGCAGC 
GACGCCGCGG CCGCCCCGGG GCAGGCAGAG GCCGATGACT CTGGCGTGGG GCAAAGCTCG 
GACCGCGGCA GCCGTTCTCA GGAGGAGGTA TCTGAGAGCA GCTCGAGCGC AGACCCCCTG 
CCTAATAGCT ACCTCCCTGA TTCATCGTCT GTGTCTCATG GGCCAGTGGC AGGGGTGACA 
GGCGGTCCCC CAGCACTTGT GCACTCTAGT GCACTCCCAG ACCCCAACAT GCTGGTGTCC 
GACTGCACAG CTTCCTCCTC GGACCTGGGC TCGGCCATCG ACAAGATCAT CGAGTCCACC 
ATCGGGCCCG ACCTCATCCA GAACTGCATC ACTGTGACCA GTGCTGAGGA TGGCGGGGCC 
GAGACCACAC GGTACCTGAT CCTACAGGGC CCAGATGATG GAGCCCCCAT GACATCACCA 
ATGTCCAGTT CCACCTTGGC CCACAGCCTA GCAGCCATTG AGGCCCTGGC AGATGGCCCC 
ACATCCACAT CCACATGCCT GGAGGCACAG GGTGGGCCCA GCTCCCCGGT GCAGCTGCCC 
CCAGCCTCCG GTGCCGAAGA GCCGGACCTG CAGAGCCTGG AGGCCATGAT GGAGGTGGTG 
GTGGTGCAGC AGTTCAAATG CAAGATGTGC CAGTACCGGA GCAGCACCAA GGCCACACTG 
CTGCGCCACA TGCGGGAACG CCACTTCCGT CCAGTAGCAG CAGCCGCAGC AGCAGCTGGT 
AAAAAAGGAC GTCTACGGAA GTGGAGCACC TCCACCAAGA GCCAAGAGGA AGAGGGACCA 
GAGGAGGAGG ACGATGATGA CATTGTAGAC GCTGGAGCCA TTGATGACCT GGAGGAGGAT 
AGCGACTATA ATCCAGCTGA GGATGAGCCC CGAGGCCGGC AGCTTCGGCT CCAGCGCCCC 
ACCCCCAGTA CCCCAAGGCC CCGAAGGAGA CCTGGCCGGC CCCGGAAGCT GCCCCGCCTG 
GAGATCTCAG ACCTCCCAGA TGGTGTGGAA GGAGAGCCTC TAGTGAGTTC CCAGAGTGGA 
CAGAGCCCTC CAGAGCCACA GGATCCCGAG GCTCCCAGCT CCTCAGGCCC AGGACACCTG 
GTGGCCATGG GCAAGGTGAG CAGGACCCCT GTGGAAGCTG GTGTGAGCCA GTCAGATGCA 
GAGAACGCAG CCCCCTCCTG CCCGGATGAG CATGACACTC TGCCCCGGCG CCGAGGTCGA 
CCTTCCAGGC GCTTCCTAGG CAAGAAATAC CGCAAGTACT ATTACAAGTC GCCCAAACCA 
CTTTTGAGGC CCTTCCTGTG CCGCATCTGT GGTTCTCGCT TTCTGTCCCA CGAGGACCTG 
CGCTTCCACG TCAACTCCCA TGAGGCTGGC GATCCCCAGC TCTTCAAGTG CCTGCAGTGC 
AGCTATCGTT CCCGCCGCTG GTCCTCGCTC AAGGAGCACA TGTTCAACCA CGTGGGCAGC 
AAGCCCTACA AGTGTGACGA GTGCAGCTAC ACCAGTGTCT ACCGGAAGGA CGTCATTCGG 
CACGCCGCTG TGCACAGCCG GGACCGGAAG AAGAGGCCAG ATCCGACTCC AAAGCTGAGC 
TCTTTCCCCT GCCCTGTGTG TGGCCGTGTG TACCCCATGC AGAAAAGACT CACGCAGCAC 
ATGAAGACGC ACAGCACTGA GAAGCCCCAC ATGTGTGACA AGTGTGGAAA GTCCTTTAAG 
AAGCGCTACA CCTTCAAAAT GCACCTGCTC ACGCACATCC AGGCTGTTGC CAACCGCAGG 
TTCAAGTGTG AGTTCTGTGA GTTTGTTTGT GAAGACAAGA AGGCACTGCT GAACCACCAG 
TTGTCCCACG TCAGTGACAA GCCCTTCAAA TGCAGCTTTT GTCCCTACCG CACCTTCCGA 
GAGGACTTCT TGCTGTCCCA TGTGGCTGTC AAGCACACAG GGGCCAAGCC CTTCGCCTGT 
GAGTACTGCC ACTTCAGCAC ACGGCACAAG AAGAACCTGC GCCTGCACGT ACGGTGCCGA 
CACGCAAGCA GCTTCGAGGA ATGGGGGAGG CGCCACCCTG AGGAGCCCCC CTCCCGCCGT 
CGCCCCTTCT TCTCTCTGCA GCAGATTGAG GAGCTGAAGC AGCAGCACAG TGCGGCCCCT 
GGACCACCTC CCAGTTCCCC AGGACCTCCT GAGATACCCC CAGAGGCGAC AACTTTCCAG 
TCATCTGAGG CTCCCTCATT GCTCTGTTCT GACACCCTGG GCGGCGCCAC CATCATCTAC 
CAGCAAGGAG CTGAGGAGTC GACAGCGATG GCCACGCAGA CAGCCTTGGA TCTTCTGCTG 
AACATGAGTG CTCAGCGGGA ACTGGGGGGC ACAGCCCTGC AGGTGGCTGT GGTGAAGTCG 
GAAGATGTGG AAGCAGGGTT AGCATCCCCT GGTGGGCAGC CCTCCCCTGA AGGTGCCACT 
CCACAGGTGG TCACCCTCCA CGTGGCAGAG CCAGGGGGCG GTGCAGCAGC CGAGAGCCAG 
CTAGGCCCTC CTGACCTACC GCAGATCACC CTGGCACCTG GTCCATTTGG TGGGACTGGC 
TACAGTGTCA TCACAGCACC CCCTATGGAG GAGGGAACAT CAGCTCCTGG CACACCTTAC 
AGGGGGCTCC TGTGGCCCTC CATCTTCTCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF335 ( XM_011528979.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011527281.1 Homo sapiens zinc finger protein 335 (ZNF335), transcript variant X4, mRNA.

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CloneID	OHu71155
Clone ID Related Accession (Same CDS sequence)	XM_011528979.2 , XM_011528979.3
Accession Version	XM_011528979.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	zinc finger protein 335 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011528979.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACGAGGTGGA GAGCAGCAGC GACGCGGCCC CTGGGCCTGG CCGGCCCGAG 
GAGCCCTCTG AGAGCGGCCT GGGTGTGGGC ACCTCAGAAG CCGTGTCCGC CGACAGCAGC 
GACGCCGCGG CCGCCCCGGG GCAGGCAGAG GCCGATGACT CTGGCGTGGG GCAAAGCTCG 
GACCGCGGCA GCCGTTCTCA GGAGGAGGTA TCTGAGAGCA GCTCGAGCGC AGACCCCCTG 
CCTAATAGCT ACCTCCCTGA TTCATCGTCT GTGTCTCATG GGCCAGTGGC AGGGGTGACA 
GGCGGTCCCC CAGCACTTGT GCACTCTAGT GCACTCCCAG ACCCCAACAT GCTGGTGTCC 
GACTGCACAG CTTCCTCCTC GGACCTGGGC TCGGCCATCG ACAAGATCAT CGAGTCCACC 
ATCGGGCCCG ACCTCATCCA GAACTGCATC ACTGTGACCA GTGCTGAGGA TGGCGGGGCC 
GAGACCACAC GGTACCTGAT CCTACAGGGC CCAGATGATG GAGCCCCCAT GACATCACCA 
ATGTCCAGTT CCACCTTGGC CCACAGCCTA GCAGCCATTG AGGCCCTGGC AGATGGCCCC 
ACATCCACAT CCACATGCCT GGAGGCACAG GGTGGGCCCA GCTCCCCGGT GCAGCTGCCC 
CCAGCCTCCG GTGCCGAAGA GCCGGACCTG CAGAGCCTGG AGGCCATGAT GGAGGTGGTG 
GTGGTGCAGC AGTTCAAATG CAAGATGTGC CAGTACCGGA GCAGCACCAA GGCCACACTG 
CTGCGCCACA TGCGGGAACG CCACTTCCGT CCAGTAGCAG CAGCCGCAGC AGCAGCTGGT 
AAAAAAGGAC GTCTACGGAA GTGGAGCACC TCCACCAAGA GCCAAGAGGA AGAGGGACCA 
GAGGAGGAGG ACGATGATGA CATTGTAGAC GCTGGAGCCA TTGATGACCT GGAGGAGGAT 
AGCGACTATA ATCCAGCTGA GGATGAGCCC CGAGGCCGGC AGCTTCGGCT CCAGCGCCCC 
ACCCCCAGTA CCCCAAGGCC CCGAAGGAGA CCTGGCCGGC CCCGGAAGCT GCCCCGCCTG 
GAGATCTCAG ACCTCCCAGA TGGTGTGGAA GGAGAGCCTC TAGTGAGTTC CCAGAGTGGA 
CAGAGCCCTC CAGAGCCACA GGATCCCGAG GCTCCCAGCT CCTCAGGCCC AGGACACCTG 
GTGGCCATGG GCAAGGTGAG CAGGACCCCT GTGGAAGCTG GTGTGAGCCA GTCAGATGCA 
GAGAACGCAG CCCCCTCCTG CCCGGATGAG CATGACACTC TGCCCCGGCG CCGAGGTCGA 
CCTTCCAGGC GCTTCCTAGG CAAGAAATAC CGCAAGTACT ATTACAAGTC GCCCAAACCA 
CTTTTGAGGC CCTTCCTGTG CCGCATCTGT GGTTCTCGCT TTCTGTCCCA CGAGGACCTG 
CGCTTCCACG TCAACTCCCA TGAGGCTGGC GATCCCCAGC TCTTCAAGTG CCTGCAGTGC 
AGCTATCGTT CCCGCCGCTG GTCCTCGCTC AAGGAGCACA TGTTCAACCA CGTGGGCAGC 
AAGCCCTACA AGTGTGACGA GTGCAGCTAC ACCAGTGTCT ACCGGAAGGA CGTCATTCGG 
CACGCCGCTG TGCACAGCCG GGACCGGAAG AAGAGGCCAG ATCCGACTCC AAAGCTGAGC 
TCTTTCCCCT GCCCTGTGTG TGGCCGTGTG TACCCCATGC AGAAAAGACT CACGCAGCAC 
ATGAAGACGC ACAGCACTGA GAAGCCCCAC ATGTGTGACA AGTGTGGAAA GTCCTTTAAG 
AAGCGCTACA CCTTCAAAAT GCACCTGCTC ACGCACATCC AGGCTGTTGC CAACCGCAGG 
TTCAAGTGTG AGTTCTGTGA GTTTGTTTGT GAAGACAAGA AGGCACTGCT GAACCACCAG 
TTGTCCCACG TCAGTGACAA GCCCTTCAAA TGCAGCTTTT GTCCCTACCG CACCTTCCGA 
GAGGACTTCT TGCTGTCCCA TGTGGCTGTC AAGCACACAG GGGCCAAGCC CTTCGCCTGT 
GAGTACTGCC ACTTCAGCAC ACGGCACAAG AAGAACCTGC GCCTGCACGT ACGGTGCCGA 
CACGCAAGCA GCTTCGAGGA ATGGGGGAGG CGCCACCCTG AGGAGCCCCC CTCCCGCCGT 
CGCCCCTTCT TCTCTCTGCA GCAGATTGAG GAGCTGAAGC AGCAGCACAG TGCGGCCCCT 
GGACCACCTC CCAGTTCCCC AGGACCTCCT GAGATACCCC CAGAGGCGAC AACTTTCCAG 
TCATCTGAGG CTCCCTCATT GCTCTGTTCT GACACCCTGG GCGGCGCCAC CATCATCTAC 
CAGCAAGGAG CTGAGGAGTC GACAGCGATG GCCACGCAGA CAGCCTTGGA TCTTCTGCTG 
AACATGAGTG CTCAGCGGGA ACTGGGGGGC ACAGCCCTGC AGGTGGCTGT GGTGAAGTCG 
GAAGATGTGG AAGCAGGGTT AGCATCCCCT GGTGGGCAGC CCTCCCCTGA AGGTGCCACT 
CCACAGGTGG TCACCCTCCA CGTGGCAGAG CCAGGGGGCG GTGCAGCAGC CGAGAGCCAG 
CTAGGCCCTC CTGACCTACC GCAGATCACC CTGGCACCTG GTCCATTTGG TGGGACTGGC 
TACAGTGTCA TCACAGCACC CCCTATGGAG GAGGGAACAT CAGCTCCTGG CACACCTTAC 
AGGGGGCTCC TGTGGCCCTC CATCTTCTCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011527281.1
CDS	493..3225
Translation	MEENEVESSSDAAPGPGRPEEPSESGLGVGTSEAVSADSSDAAAAPGQAEADDSGVGQSSDRGSRSQEEVSESSSSADPLPNSYLPDSSSVSHGPVAGVTGGPPALVHSSALPDPNMLVSDCTASSSDLGSAIDKIIESTIGPDLIQNCITVTSAEDGGAETTRYLILQGPDDGAPMTSPMSSSTLAHSLAAIEALADGPTSTSTCLEAQGGPSSPVQLPPASGAEEPDLQSLEAMMEVVVVQQFKCKMCQYRSSTKATLLRHMRERHFRPVAAAAAAAGKKGRLRKWSTSTKSQEEEGPEEEDDDDIVDAGAIDDLEEDSDYNPAEDEPRGRQLRLQRPTPSTPRPRRRPGRPRKLPRLEISDLPDGVEGEPLVSSQSGQSPPEPQDPEAPSSSGPGHLVAMGKVSRTPVEAGVSQSDAENAAPSCPDEHDTLPRRRGRPSRRFLGKKYRKYYYKSPKPLLRPFLCRICGSRFLSHEDLRFHVNSHEAGDPQLFKCLQCSYRSRRWSSLKEHMFNHVGSKPYKCDECSYTSVYRKDVIRHAAVHSRDRKKRPDPTPKLSSFPCPVCGRVYPMQKRLTQHMKTHSTEKPHMCDKCGKSFKKRYTFKMHLLTHIQAVANRRFKCEFCEFVCEDKKALLNHQLSHVSDKPFKCSFCPYRTFREDFLLSHVAVKHTGAKPFACEYCHFSTRHKKNLRLHVRCRHASSFEEWGRRHPEEPPSRRRPFFSLQQIEELKQQHSAAPGPPPSSPGPPEIPPEATTFQSSEAPSLLCSDTLGGATIIYQQGAEESTAMATQTALDLLLNMSAQRELGGTALQVAVVKSEDVEAGLASPGGQPSPEGATPQVVTLHVAEPGGGAAAESQLGPPDLPQITLAPGPFGGTGYSVITAPPMEEGTSAPGTPYRGLLWPSIFS

Target ORF information:

RefSeq Version	XM_011528979.3
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 335 (ZNF335), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528979.3

ORF Insert Sequence:

ATGGAGGAGA ACGAGGTGGA GAGCAGCAGC GACGCGGCCC CTGGGCCTGG CCGGCCCGAG 
GAGCCCTCTG AGAGCGGCCT GGGTGTGGGC ACCTCAGAAG CCGTGTCCGC CGACAGCAGC 
GACGCCGCGG CCGCCCCGGG GCAGGCAGAG GCCGATGACT CTGGCGTGGG GCAAAGCTCG 
GACCGCGGCA GCCGTTCTCA GGAGGAGGTA TCTGAGAGCA GCTCGAGCGC AGACCCCCTG 
CCTAATAGCT ACCTCCCTGA TTCATCGTCT GTGTCTCATG GGCCAGTGGC AGGGGTGACA 
GGCGGTCCCC CAGCACTTGT GCACTCTAGT GCACTCCCAG ACCCCAACAT GCTGGTGTCC 
GACTGCACAG CTTCCTCCTC GGACCTGGGC TCGGCCATCG ACAAGATCAT CGAGTCCACC 
ATCGGGCCCG ACCTCATCCA GAACTGCATC ACTGTGACCA GTGCTGAGGA TGGCGGGGCC 
GAGACCACAC GGTACCTGAT CCTACAGGGC CCAGATGATG GAGCCCCCAT GACATCACCA 
ATGTCCAGTT CCACCTTGGC CCACAGCCTA GCAGCCATTG AGGCCCTGGC AGATGGCCCC 
ACATCCACAT CCACATGCCT GGAGGCACAG GGTGGGCCCA GCTCCCCGGT GCAGCTGCCC 
CCAGCCTCCG GTGCCGAAGA GCCGGACCTG CAGAGCCTGG AGGCCATGAT GGAGGTGGTG 
GTGGTGCAGC AGTTCAAATG CAAGATGTGC CAGTACCGGA GCAGCACCAA GGCCACACTG 
CTGCGCCACA TGCGGGAACG CCACTTCCGT CCAGTAGCAG CAGCCGCAGC AGCAGCTGGT 
AAAAAAGGAC GTCTACGGAA GTGGAGCACC TCCACCAAGA GCCAAGAGGA AGAGGGACCA 
GAGGAGGAGG ACGATGATGA CATTGTAGAC GCTGGAGCCA TTGATGACCT GGAGGAGGAT 
AGCGACTATA ATCCAGCTGA GGATGAGCCC CGAGGCCGGC AGCTTCGGCT CCAGCGCCCC 
ACCCCCAGTA CCCCAAGGCC CCGAAGGAGA CCTGGCCGGC CCCGGAAGCT GCCCCGCCTG 
GAGATCTCAG ACCTCCCAGA TGGTGTGGAA GGAGAGCCTC TAGTGAGTTC CCAGAGTGGA 
CAGAGCCCTC CAGAGCCACA GGATCCCGAG GCTCCCAGCT CCTCAGGCCC AGGACACCTG 
GTGGCCATGG GCAAGGTGAG CAGGACCCCT GTGGAAGCTG GTGTGAGCCA GTCAGATGCA 
GAGAACGCAG CCCCCTCCTG CCCGGATGAG CATGACACTC TGCCCCGGCG CCGAGGTCGA 
CCTTCCAGGC GCTTCCTAGG CAAGAAATAC CGCAAGTACT ATTACAAGTC GCCCAAACCA 
CTTTTGAGGC CCTTCCTGTG CCGCATCTGT GGTTCTCGCT TTCTGTCCCA CGAGGACCTG 
CGCTTCCACG TCAACTCCCA TGAGGCTGGC GATCCCCAGC TCTTCAAGTG CCTGCAGTGC 
AGCTATCGTT CCCGCCGCTG GTCCTCGCTC AAGGAGCACA TGTTCAACCA CGTGGGCAGC 
AAGCCCTACA AGTGTGACGA GTGCAGCTAC ACCAGTGTCT ACCGGAAGGA CGTCATTCGG 
CACGCCGCTG TGCACAGCCG GGACCGGAAG AAGAGGCCAG ATCCGACTCC AAAGCTGAGC 
TCTTTCCCCT GCCCTGTGTG TGGCCGTGTG TACCCCATGC AGAAAAGACT CACGCAGCAC 
ATGAAGACGC ACAGCACTGA GAAGCCCCAC ATGTGTGACA AGTGTGGAAA GTCCTTTAAG 
AAGCGCTACA CCTTCAAAAT GCACCTGCTC ACGCACATCC AGGCTGTTGC CAACCGCAGG 
TTCAAGTGTG AGTTCTGTGA GTTTGTTTGT GAAGACAAGA AGGCACTGCT GAACCACCAG 
TTGTCCCACG TCAGTGACAA GCCCTTCAAA TGCAGCTTTT GTCCCTACCG CACCTTCCGA 
GAGGACTTCT TGCTGTCCCA TGTGGCTGTC AAGCACACAG GGGCCAAGCC CTTCGCCTGT 
GAGTACTGCC ACTTCAGCAC ACGGCACAAG AAGAACCTGC GCCTGCACGT ACGGTGCCGA 
CACGCAAGCA GCTTCGAGGA ATGGGGGAGG CGCCACCCTG AGGAGCCCCC CTCCCGCCGT 
CGCCCCTTCT TCTCTCTGCA GCAGATTGAG GAGCTGAAGC AGCAGCACAG TGCGGCCCCT 
GGACCACCTC CCAGTTCCCC AGGACCTCCT GAGATACCCC CAGAGGCGAC AACTTTCCAG 
TCATCTGAGG CTCCCTCATT GCTCTGTTCT GACACCCTGG GCGGCGCCAC CATCATCTAC 
CAGCAAGGAG CTGAGGAGTC GACAGCGATG GCCACGCAGA CAGCCTTGGA TCTTCTGCTG 
AACATGAGTG CTCAGCGGGA ACTGGGGGGC ACAGCCCTGC AGGTGGCTGT GGTGAAGTCG 
GAAGATGTGG AAGCAGGGTT AGCATCCCCT GGTGGGCAGC CCTCCCCTGA AGGTGCCACT 
CCACAGGTGG TCACCCTCCA CGTGGCAGAG CCAGGGGGCG GTGCAGCAGC CGAGAGCCAG 
CTAGGCCCTC CTGACCTACC GCAGATCACC CTGGCACCTG GTCCATTTGG TGGGACTGGC 
TACAGTGTCA TCACAGCACC CCCTATGGAG GAGGGAACAT CAGCTCCTGG CACACCTTAC 
AGGGGGCTCC TGTGGCCCTC CATCTTCTCC TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF335 ( XM_017028012.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016883501.1 Homo sapiens zinc finger protein 335 (ZNF335), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu53086
Clone ID Related Accession (Same CDS sequence)	XM_017028012.1
Accession Version	XM_017028012.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	zinc finger protein 335 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCAC CAATGTCCAG TTCCACCTTG GCCCACAGCC TAGCAGCCAT TGAGGCCCTG 
GCAGATGGCC CCACATCCAC ATCCACATGC CTGGAGGCAC AGGGTGGGCC CAGCTCCCCG 
GTGCAGCTGC CCCCAGCCTC CGGTGCCGAA GAGCCGGACC TGCAGAGCCT GGAGGCCATG 
ATGGAGGTGG TGGTGGTGCA GCAGTTCAAA TGCAAGATGT GCCAGTACCG GAGCAGCACC 
AAGGCCACAC TGCTGCGCCA CATGCGGGAA CGCCACTTCC GTCCAGTAGC AGCAGCCGCA 
GCAGCAGCTG GTAAAAAAGG ACGTCTACGG AAGTGGAGCA CCTCCACCAA GAGCCAAGAG 
GAAGAGGGAC CAGAGGAGGA GGACGATGAT GACATTGTAG ACGCTGGAGC CATTGATGAC 
CTGGAGGAGG ATAGCGACTA TAATCCAGCT GAGGATGAGC CCCGAGGCCG GCAGCTTCGG 
CTCCAGCGCC CCACCCCCAG TACCCCAAGG CCCCGAAGGA GACCTGGCCG GCCCCGGAAG 
CTGCCCCGCC TGGAGATCTC AGACCTCCCA GATGGTGTGG AAGGAGAGCC TCTAGTGAGT 
TCCCAGAGTG GACAGAGCCC TCCAGAGCCA CAGGATCCCG AGGCTCCCAG CTCCTCAGGC 
CCAGGACACC TGGTGGCCAT GGGCAAGGTG AGCAGGACCC CTGTGGAAGC TGGTGTGAGC 
CAGTCAGATG CAGAGAACGC AGCCCCCTCC TGCCCGGATG AGCATGACAC TCTGCCCCGG 
CGCCGAGGTC GACCTTCCAG GCGCTTCCTA GGCAAGAAAT ACCGCAAGTA CTATTACAAG 
TCGCCCAAAC CACTTTTGAG GCCCTTCCTG TGCCGCATCT GTGGTTCTCG CTTTCTGTCC 
CACGAGGACC TGCGCTTCCA CGTCAACTCC CATGAGGCTG GCGATCCCCA GCTCTTCAAG 
TGCCTGCAGT GCAGCTATCG TTCCCGCCGC TGGTCCTCGC TCAAGGAGCA CATGTTCAAC 
CACGTGGGCA GCAAGCCCTA CAAGTGTGAC GAGTGCAGCT ACACCAGTGT CTACCGGAAG 
GACGTCATTC GGCACGCCGC TGTGCACAGC CGGGACCGGA AGAAGAGGCC AGATCCGACT 
CCAAAGCTGA GCTCTTTCCC CTGCCCTGTG TGTGGCCGTG TGTACCCCAT GCAGAAAAGA 
CTCACGCAGC ACATGAAGAC GCACAGCACT GAGAAGCCCC ACATGTGTGA CAAGTGTGGA 
AAGTCCTTTA AGAAGCGCTA CACCTTCAAA ATGCACCTGC TCACGCACAT CCAGGCTGTT 
GCCAACCGCA GGTTCAAGTG TGAGTTCTGT GAGTTTGTTT GTGAAGACAA GAAGGCACTG 
CTGAACCACC AGTTGTCCCA CGTCAGTGAC AAGCCCTTCA AATGCAGCTT TTGTCCCTAC 
CGCACCTTCC GAGAGGACTT CTTGCTGTCC CATGTGGCTG TCAAGCACAC AGGGGCCAAG 
CCCTTCGCCT GTGAGTACTG CCACTTCAGC ACACGGCACA AGAAGAACCT GCGCCTGCAC 
GTACGGTGCC GACACGCAAG CAGCTTCGAG GAATGGGGGA GGCGCCACCC TGAGGAGCCC 
CCCTCCCGCC GTCGCCCCTT CTTCTCTCTG CAGCAGATTG AGGAGCTGAA GCAGCAGCAC 
AGTGCGGCCC CTGGACCACC TCCCAGTTCC CCAGGACCTC CTGAGATACC CCCAGAGGCG 
ACAACTTTCC AGTCATCTGA GGCTCCCTCA TTGCTCTGTT CTGACACCCT GGGCGGCGCC 
ACCATCATCT ACCAGCAAGG AGCTGAGGAG TCGACAGCGA TGGCCACGCA GACAGCCTTG 
GATCTTCTGC TGAACATGAG TGCTCAGCGG GAACTGGGGG GCACAGCCCT GCAGGTGGCT 
GTGGTGAAGT CGGAAGATGT GGAAGCAGGG TTAGCATCCC CTGGTGGGCA GCCCTCCCCT 
GAAGGTGCCA CTCCACAGGT GGTCACCCTC CACGTGGCAG AGCCAGGGGG CGGTGCAGCA 
GCCGAGAGCC AGCTAGGCCC TCCTGACCTA CCGCAGATCA CCCTGGCACC TGGTCCATTT 
GGTGGGACTG GCTACAGTGT CATCACAGCA CCCCCTATGG AGGAGGGAAC ATCAGCTCCT 
GGCACACCTT ACAGCGAGGA GCCCGCAGGA GAGGCAGCCC AGGCTGTGGT TGTGAGTGAC 
ACCCTAAAAG AAGCTGGCAC CCACTACATC ATGGCTACTG ATGGTACCCA GTTGCACCAC 
ATTGAGCTCA CCGCAGATGG CTCCATCTCC TTCCCAAGTC CAGATGCTCT GGCCTCTGGT 
GCCAAATGGC CCCTGCTGCA GTGTGGGGGA CTGCCCAGAG ACGGCCCTGA GCCCCCATCT 
CCAGCCAAGA CCCACTGCGT AGGGGACTCC CAGAGCTCTG CCTCCTCACC TCCTGCAACC 
AGCAAAGCCC TGGGCCTGGC AGTGCCCCCG TCACCGCCAT CTGCAGCCAC TGCTGCATCA 
AAGAAGTTTT CCTGCAAGAT CTGTGCCGAG GCCTTCCCTG GCCGAGCTGA GATGGAGAGT 
CACAAGCGGG CCCACGCTGG GCCTGGTGCC TTCAAGTGCC CCGACTGCCC CTTCAGTGCC 
CGCCAGTGGC CCGAGGTCCG GGCGCACATG GCACAGCACT CAAGCCTACG GCCCCACCAG 
TGTAGCCAGT GCAGCTTTGC CTCCAAGAAC AAGAAGGACC TGCGTCGGCA CATGCTGACT 
CACACAAAGG AGAAGCCTTT TGCATGCCAC CTCTGCGGGC AGCGTTTCAA CCGTAACGGG 
CACCTCAAGT TCCACATCCA GCGGCTGCAC AGTCCTGATG GGAGGAAGTC AGGAACCCCT 
ACAGCCCGGG CCCCTACCCA GACCCCAACC CAGACCATCA TCCTGAACAG TGATGACGAA 
ACACTGGCCA CCCTGCACAC TGCACTCCAG TCCAGTCACG GGGTCCTGGG CCCAGAGCGG 
CTACAGCAGG CACTGAGCCA GGAACACATC ATCGTTGCCC AGGAACAGAC AGTGACCAAT 
CAGGAGGAAG CCGCCTACAT CCAAGAGATC ACCACGGCAG ATGGCCAGAC CGTACAGCAC 
CTGGTGACCT CCGACAACCA GGTGCAGTAT ATCATCTCCC AGGATGGTGT CCAGCACCTG 
CTCCCCCAGG AATATGTTGT GGTCCCTGAA GGCCATCACA TCCAGGTACA GGAGGGCCAG 
ATCACACACA TCCAGTATGA ACAAGGAGCC CCGTTCCTTC AGGAGTCCCA GATCCAGTAT 
GTGCCTGTGT CCCCAGGCCA GCAGCTTGTC ACACAGGCTC AACTTGAGGC TGCAGCACAC 
TCAGCTGTCA CAGCAGTGGC TGATGCTGCC ATGGCCCAAG CCCAGGGCCT GTTTGGTACA 
GACGAGACAG TGCCCGAACA CATTCAACAG CTGCAGCACC AGGGCATCGA GTACGACGTC 
ATCACCCTGG CCGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016883501.1
CDS	423..3923
Translation	MTSPMSSSTLAHSLAAIEALADGPTSTSTCLEAQGGPSSPVQLPPASGAEEPDLQSLEAMMEVVVVQQFKCKMCQYRSSTKATLLRHMRERHFRPVAAAAAAAGKKGRLRKWSTSTKSQEEEGPEEEDDDDIVDAGAIDDLEEDSDYNPAEDEPRGRQLRLQRPTPSTPRPRRRPGRPRKLPRLEISDLPDGVEGEPLVSSQSGQSPPEPQDPEAPSSSGPGHLVAMGKVSRTPVEAGVSQSDAENAAPSCPDEHDTLPRRRGRPSRRFLGKKYRKYYYKSPKPLLRPFLCRICGSRFLSHEDLRFHVNSHEAGDPQLFKCLQCSYRSRRWSSLKEHMFNHVGSKPYKCDECSYTSVYRKDVIRHAAVHSRDRKKRPDPTPKLSSFPCPVCGRVYPMQKRLTQHMKTHSTEKPHMCDKCGKSFKKRYTFKMHLLTHIQAVANRRFKCEFCEFVCEDKKALLNHQLSHVSDKPFKCSFCPYRTFREDFLLSHVAVKHTGAKPFACEYCHFSTRHKKNLRLHVRCRHASSFEEWGRRHPEEPPSRRRPFFSLQQIEELKQQHSAAPGPPPSSPGPPEIPPEATTFQSSEAPSLLCSDTLGGATIIYQQGAEESTAMATQTALDLLLNMSAQRELGGTALQVAVVKSEDVEAGLASPGGQPSPEGATPQVVTLHVAEPGGGAAAESQLGPPDLPQITLAPGPFGGTGYSVITAPPMEEGTSAPGTPYSEEPAGEAAQAVVVSDTLKEAGTHYIMATDGTQLHHIELTADGSISFPSPDALASGAKWPLLQCGGLPRDGPEPPSPAKTHCVGDSQSSASSPPATSKALGLAVPPSPPSAATAASKKFSCKICAEAFPGRAEMESHKRAHAGPGAFKCPDCPFSARQWPEVRAHMAQHSSLRPHQCSQCSFASKNKKDLRRHMLTHTKEKPFACHLCGQRFNRNGHLKFHIQRLHSPDGRKSGTPTARAPTQTPTQTIILNSDDETLATLHTALQSSHGVLGPERLQQALSQEHIIVAQEQTVTNQEEAAYIQEITTADGQTVQHLVTSDNQVQYIISQDGVQHLLPQEYVVVPEGHHIQVQEGQITHIQYEQGAPFLQESQIQYVPVSPGQQLVTQAQLEAAAHSAVTAVADAAMAQAQGLFGTDETVPEHIQQLQHQGIEYDVITLADD

Target ORF information:

RefSeq Version	XM_017028012.1
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 335 (ZNF335), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017028012.1

ORF Insert Sequence:

ATGACATCAC CAATGTCCAG TTCCACCTTG GCCCACAGCC TAGCAGCCAT TGAGGCCCTG 
GCAGATGGCC CCACATCCAC ATCCACATGC CTGGAGGCAC AGGGTGGGCC CAGCTCCCCG 
GTGCAGCTGC CCCCAGCCTC CGGTGCCGAA GAGCCGGACC TGCAGAGCCT GGAGGCCATG 
ATGGAGGTGG TGGTGGTGCA GCAGTTCAAA TGCAAGATGT GCCAGTACCG GAGCAGCACC 
AAGGCCACAC TGCTGCGCCA CATGCGGGAA CGCCACTTCC GTCCAGTAGC AGCAGCCGCA 
GCAGCAGCTG GTAAAAAAGG ACGTCTACGG AAGTGGAGCA CCTCCACCAA GAGCCAAGAG 
GAAGAGGGAC CAGAGGAGGA GGACGATGAT GACATTGTAG ACGCTGGAGC CATTGATGAC 
CTGGAGGAGG ATAGCGACTA TAATCCAGCT GAGGATGAGC CCCGAGGCCG GCAGCTTCGG 
CTCCAGCGCC CCACCCCCAG TACCCCAAGG CCCCGAAGGA GACCTGGCCG GCCCCGGAAG 
CTGCCCCGCC TGGAGATCTC AGACCTCCCA GATGGTGTGG AAGGAGAGCC TCTAGTGAGT 
TCCCAGAGTG GACAGAGCCC TCCAGAGCCA CAGGATCCCG AGGCTCCCAG CTCCTCAGGC 
CCAGGACACC TGGTGGCCAT GGGCAAGGTG AGCAGGACCC CTGTGGAAGC TGGTGTGAGC 
CAGTCAGATG CAGAGAACGC AGCCCCCTCC TGCCCGGATG AGCATGACAC TCTGCCCCGG 
CGCCGAGGTC GACCTTCCAG GCGCTTCCTA GGCAAGAAAT ACCGCAAGTA CTATTACAAG 
TCGCCCAAAC CACTTTTGAG GCCCTTCCTG TGCCGCATCT GTGGTTCTCG CTTTCTGTCC 
CACGAGGACC TGCGCTTCCA CGTCAACTCC CATGAGGCTG GCGATCCCCA GCTCTTCAAG 
TGCCTGCAGT GCAGCTATCG TTCCCGCCGC TGGTCCTCGC TCAAGGAGCA CATGTTCAAC 
CACGTGGGCA GCAAGCCCTA CAAGTGTGAC GAGTGCAGCT ACACCAGTGT CTACCGGAAG 
GACGTCATTC GGCACGCCGC TGTGCACAGC CGGGACCGGA AGAAGAGGCC AGATCCGACT 
CCAAAGCTGA GCTCTTTCCC CTGCCCTGTG TGTGGCCGTG TGTACCCCAT GCAGAAAAGA 
CTCACGCAGC ACATGAAGAC GCACAGCACT GAGAAGCCCC ACATGTGTGA CAAGTGTGGA 
AAGTCCTTTA AGAAGCGCTA CACCTTCAAA ATGCACCTGC TCACGCACAT CCAGGCTGTT 
GCCAACCGCA GGTTCAAGTG TGAGTTCTGT GAGTTTGTTT GTGAAGACAA GAAGGCACTG 
CTGAACCACC AGTTGTCCCA CGTCAGTGAC AAGCCCTTCA AATGCAGCTT TTGTCCCTAC 
CGCACCTTCC GAGAGGACTT CTTGCTGTCC CATGTGGCTG TCAAGCACAC AGGGGCCAAG 
CCCTTCGCCT GTGAGTACTG CCACTTCAGC ACACGGCACA AGAAGAACCT GCGCCTGCAC 
GTACGGTGCC GACACGCAAG CAGCTTCGAG GAATGGGGGA GGCGCCACCC TGAGGAGCCC 
CCCTCCCGCC GTCGCCCCTT CTTCTCTCTG CAGCAGATTG AGGAGCTGAA GCAGCAGCAC 
AGTGCGGCCC CTGGACCACC TCCCAGTTCC CCAGGACCTC CTGAGATACC CCCAGAGGCG 
ACAACTTTCC AGTCATCTGA GGCTCCCTCA TTGCTCTGTT CTGACACCCT GGGCGGCGCC 
ACCATCATCT ACCAGCAAGG AGCTGAGGAG TCGACAGCGA TGGCCACGCA GACAGCCTTG 
GATCTTCTGC TGAACATGAG TGCTCAGCGG GAACTGGGGG GCACAGCCCT GCAGGTGGCT 
GTGGTGAAGT CGGAAGATGT GGAAGCAGGG TTAGCATCCC CTGGTGGGCA GCCCTCCCCT 
GAAGGTGCCA CTCCACAGGT GGTCACCCTC CACGTGGCAG AGCCAGGGGG CGGTGCAGCA 
GCCGAGAGCC AGCTAGGCCC TCCTGACCTA CCGCAGATCA CCCTGGCACC TGGTCCATTT 
GGTGGGACTG GCTACAGTGT CATCACAGCA CCCCCTATGG AGGAGGGAAC ATCAGCTCCT 
GGCACACCTT ACAGCGAGGA GCCCGCAGGA GAGGCAGCCC AGGCTGTGGT TGTGAGTGAC 
ACCCTAAAAG AAGCTGGCAC CCACTACATC ATGGCTACTG ATGGTACCCA GTTGCACCAC 
ATTGAGCTCA CCGCAGATGG CTCCATCTCC TTCCCAAGTC CAGATGCTCT GGCCTCTGGT 
GCCAAATGGC CCCTGCTGCA GTGTGGGGGA CTGCCCAGAG ACGGCCCTGA GCCCCCATCT 
CCAGCCAAGA CCCACTGCGT AGGGGACTCC CAGAGCTCTG CCTCCTCACC TCCTGCAACC 
AGCAAAGCCC TGGGCCTGGC AGTGCCCCCG TCACCGCCAT CTGCAGCCAC TGCTGCATCA 
AAGAAGTTTT CCTGCAAGAT CTGTGCCGAG GCCTTCCCTG GCCGAGCTGA GATGGAGAGT 
CACAAGCGGG CCCACGCTGG GCCTGGTGCC TTCAAGTGCC CCGACTGCCC CTTCAGTGCC 
CGCCAGTGGC CCGAGGTCCG GGCGCACATG GCACAGCACT CAAGCCTACG GCCCCACCAG 
TGTAGCCAGT GCAGCTTTGC CTCCAAGAAC AAGAAGGACC TGCGTCGGCA CATGCTGACT 
CACACAAAGG AGAAGCCTTT TGCATGCCAC CTCTGCGGGC AGCGTTTCAA CCGTAACGGG 
CACCTCAAGT TCCACATCCA GCGGCTGCAC AGTCCTGATG GGAGGAAGTC AGGAACCCCT 
ACAGCCCGGG CCCCTACCCA GACCCCAACC CAGACCATCA TCCTGAACAG TGATGACGAA 
ACACTGGCCA CCCTGCACAC TGCACTCCAG TCCAGTCACG GGGTCCTGGG CCCAGAGCGG 
CTACAGCAGG CACTGAGCCA GGAACACATC ATCGTTGCCC AGGAACAGAC AGTGACCAAT 
CAGGAGGAAG CCGCCTACAT CCAAGAGATC ACCACGGCAG ATGGCCAGAC CGTACAGCAC 
CTGGTGACCT CCGACAACCA GGTGCAGTAT ATCATCTCCC AGGATGGTGT CCAGCACCTG 
CTCCCCCAGG AATATGTTGT GGTCCCTGAA GGCCATCACA TCCAGGTACA GGAGGGCCAG 
ATCACACACA TCCAGTATGA ACAAGGAGCC CCGTTCCTTC AGGAGTCCCA GATCCAGTAT 
GTGCCTGTGT CCCCAGGCCA GCAGCTTGTC ACACAGGCTC AACTTGAGGC TGCAGCACAC 
TCAGCTGTCA CAGCAGTGGC TGATGCTGCC ATGGCCCAAG CCCAGGGCCT GTTTGGTACA 
GACGAGACAG TGCCCGAACA CATTCAACAG CTGCAGCACC AGGGCATCGA GTACGACGTC 
ATCACCCTGG CCGATGACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ZNF335 ( XM_005260504.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005260561.1 Homo sapiens zinc finger protein 335 (ZNF335), transcript variant X1, mRNA.

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CloneID	OHu53085
Clone ID Related Accession (Same CDS sequence)	XM_005260504.4
Accession Version	XM_005260504.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4026bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	zinc finger protein 335 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260504.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGGAGA ACGAGGTGGA GAGCAGCAGC GACGCGGCCC CTGGGCCTGG CCGGCCCGAG 
GAGCCCTCTG AGAGCGGCCT GGGTGTGGGC ACCTCAGAAG CCGTGTCCGC CGACAGCAGC 
GACGCCGCGG CCGCCCCGGG GCAGGCAGAG GCCGATGACT CTGGCGTGGG GCAAAGCTCG 
GACCGCGGCA GCCGTTCTCA GGAGGAGGTA TCTGAGAGCA GCTCGAGCGC AGACCCCCTG 
CCTAATAGCT ACCTCCCTGA TTCATCGTCT GTGTCTCATG GGCCAGTGGC AGGGGTGACA 
GGCGGTCCCC CAGCACTTGT GCACTCTAGT GCACTCCCAG ACCCCAACAT GCTGGTGTCC 
GACTGCACAG CTTCCTCCTC GGACCTGGGC TCGGCCATCG ACAAGATCAT CGAGTCCACC 
ATCGGGCCCG ACCTCATCCA GAACTGCATC ACTGTGACCA GTGCTGAGGA TGGCGGGGCC 
GAGACCACAC GGTACCTGAT CCTACAGGGC CCAGATGATG GAGCCCCCAT GACATCACCA 
ATGTCCAGTT CCACCTTGGC CCACAGCCTA GCAGCCATTG AGGCCCTGGC AGATGGCCCC 
ACATCCACAT CCACATGCCT GGAGGCACAG GGTGGGCCCA GCTCCCCGGT GCAGCTGCCC 
CCAGCCTCCG GTGCCGAAGA GCCGGACCTG CAGAGCCTGG AGGCCATGAT GGAGGTGGTG 
GTGGTGCAGC AGTTCAAATG CAAGATGTGC CAGTACCGGA GCAGCACCAA GGCCACACTG 
CTGCGCCACA TGCGGGAACG CCACTTCCGT CCAGCAGCAG CCGCAGCAGC AGCTGGTAAA 
AAAGGACGTC TACGGAAGTG GAGCACCTCC ACCAAGAGCC AAGAGGAAGA GGGACCAGAG 
GAGGAGGACG ATGATGACAT TGTAGACGCT GGAGCCATTG ATGACCTGGA GGAGGATAGC 
GACTATAATC CAGCTGAGGA TGAGCCCCGA GGCCGGCAGC TTCGGCTCCA GCGCCCCACC 
CCCAGTACCC CAAGGCCCCG AAGGAGACCT GGCCGGCCCC GGAAGCTGCC CCGCCTGGAG 
ATCTCAGACC TCCCAGATGG TGTGGAAGGA GAGCCTCTAG TGAGTTCCCA GAGTGGACAG 
AGCCCTCCAG AGCCACAGGA TCCCGAGGCT CCCAGCTCCT CAGGCCCAGG ACACCTGGTG 
GCCATGGGCA AGGTGAGCAG GACCCCTGTG GAAGCTGGTG TGAGCCAGTC AGATGCAGAG 
AACGCAGCCC CCTCCTGCCC GGATGAGCAT GACACTCTGC CCCGGCGCCG AGGTCGACCT 
TCCAGGCGCT TCCTAGGCAA GAAATACCGC AAGTACTATT ACAAGTCGCC CAAACCACTT 
TTGAGGCCCT TCCTGTGCCG CATCTGTGGT TCTCGCTTTC TGTCCCACGA GGACCTGCGC 
TTCCACGTCA ACTCCCATGA GGCTGGCGAT CCCCAGCTCT TCAAGTGCCT GCAGTGCAGC 
TATCGTTCCC GCCGCTGGTC CTCGCTCAAG GAGCACATGT TCAACCACGT GGGCAGCAAG 
CCCTACAAGT GTGACGAGTG CAGCTACACC AGTGTCTACC GGAAGGACGT CATTCGGCAC 
GCCGCTGTGC ACAGCCGGGA CCGGAAGAAG AGGCCAGATC CGACTCCAAA GCTGAGCTCT 
TTCCCCTGCC CTGTGTGTGG CCGTGTGTAC CCCATGCAGA AAAGACTCAC GCAGCACATG 
AAGACGCACA GCACTGAGAA GCCCCACATG TGTGACAAGT GTGGAAAGTC CTTTAAGAAG 
CGCTACACCT TCAAAATGCA CCTGCTCACG CACATCCAGG CTGTTGCCAA CCGCAGGTTC 
AAGTGTGAGT TCTGTGAGTT TGTTTGTGAA GACAAGAAGG CACTGCTGAA CCACCAGTTG 
TCCCACGTCA GTGACAAGCC CTTCAAATGC AGCTTTTGTC CCTACCGCAC CTTCCGAGAG 
GACTTCTTGC TGTCCCATGT GGCTGTCAAG CACACAGGGG CCAAGCCCTT CGCCTGTGAG 
TACTGCCACT TCAGCACACG GCACAAGAAG AACCTGCGCC TGCACGTACG GTGCCGACAC 
GCAAGCAGCT TCGAGGAATG GGGGAGGCGC CACCCTGAGG AGCCCCCCTC CCGCCGTCGC 
CCCTTCTTCT CTCTGCAGCA GATTGAGGAG CTGAAGCAGC AGCACAGTGC GGCCCCTGGA 
CCACCTCCCA GTTCCCCAGG ACCTCCTGAG ATACCCCCAG AGGCGACAAC TTTCCAGTCA 
TCTGAGGCTC CCTCATTGCT CTGTTCTGAC ACCCTGGGCG GCGCCACCAT CATCTACCAG 
CAAGGAGCTG AGGAGTCGAC AGCGATGGCC ACGCAGACAG CCTTGGATCT TCTGCTGAAC 
ATGAGTGCTC AGCGGGAACT GGGGGGCACA GCCCTGCAGG TGGCTGTGGT GAAGTCGGAA 
GATGTGGAAG CAGGGTTAGC ATCCCCTGGT GGGCAGCCCT CCCCTGAAGG TGCCACTCCA 
CAGGTGGTCA CCCTCCACGT GGCAGAGCCA GGGGGCGGTG CAGCAGCCGA GAGCCAGCTA 
GGCCCTCCTG ACCTACCGCA GATCACCCTG GCACCTGGTC CATTTGGTGG GACTGGCTAC 
AGTGTCATCA CAGCACCCCC TATGGAGGAG GGAACATCAG CTCCTGGCAC ACCTTACAGC 
GAGGAGCCCG CAGGAGAGGC AGCCCAGGCT GTGGTTGTGA GTGACACCCT AAAAGAAGCT 
GGCACCCACT ACATCATGGC TACTGATGGT ACCCAGTTGC ACCACATTGA GCTCACCGCA 
GATGGCTCCA TCTCCTTCCC AAGTCCAGAT GCTCTGGCCT CTGGTGCCAA ATGGCCCCTG 
CTGCAGTGTG GGGGACTGCC CAGAGACGGC CCTGAGCCCC CATCTCCAGC CAAGACCCAC 
TGCGTAGGGG ACTCCCAGAG CTCTGCCTCC TCACCTCCTG CAACCAGCAA AGCCCTGGGC 
CTGGCAGTGC CCCCGTCACC GCCATCTGCA GCCACTGCTG CATCAAAGAA GTTTTCCTGC 
AAGATCTGTG CCGAGGCCTT CCCTGGCCGA GCTGAGATGG AGAGTCACAA GCGGGCCCAC 
GCTGGGCCTG GTGCCTTCAA GTGCCCCGAC TGCCCCTTCA GTGCCCGCCA GTGGCCCGAG 
GTCCGGGCGC ACATGGCACA GCACTCAAGC CTACGGCCCC ACCAGTGTAG CCAGTGCAGC 
TTTGCCTCCA AGAACAAGAA GGACCTGCGT CGGCACATGC TGACTCACAC AAAGGAGAAG 
CCTTTTGCAT GCCACCTCTG CGGGCAGCGT TTCAACCGTA ACGGGCACCT CAAGTTCCAC 
ATCCAGCGGC TGCACAGTCC TGATGGGAGG AAGTCAGGAA CCCCTACAGC CCGGGCCCCT 
ACCCAGACCC CAACCCAGAC CATCATCCTG AACAGTGATG ACGAAACACT GGCCACCCTG 
CACACTGCAC TCCAGTCCAG TCACGGGGTC CTGGGCCCAG AGCGGCTACA GCAGGCACTG 
AGCCAGGAAC ACATCATCGT TGCCCAGGAA CAGACAGTGA CCAATCAGGA GGAAGCCGCC 
TACATCCAAG AGATCACCAC GGCAGATGGC CAGACCGTAC AGCACCTGGT GACCTCCGAC 
AACCAGGTGC AGTATATCAT CTCCCAGGAT GGTGTCCAGC ACCTGCTCCC CCAGGAATAT 
GTTGTGGTCC CTGAAGGCCA TCACATCCAG GTACAGGAGG GCCAGATCAC ACACATCCAG 
TATGAACAAG GAGCCCCGTT CCTTCAGGAG TCCCAGATCC AGTATGTGCC TGTGTCCCCA 
GGCCAGCAGC TTGTCACACA GGCTCAACTT GAGGCTGCAG CACACTCAGC TGTCACAGCA 
GTGGCTGATG CTGCCATGGC CCAAGCCCAG GGCCTGTTTG GTACAGACGA GACAGTGCCC 
GAACACATTC AACAGCTGCA GCACCAGGGC ATCGAGTACG ACGTCATCAC CCTGGCCGAT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005260561.1
CDS	840..4865
Translation	MEENEVESSSDAAPGPGRPEEPSESGLGVGTSEAVSADSSDAAAAPGQAEADDSGVGQSSDRGSRSQEEVSESSSSADPLPNSYLPDSSSVSHGPVAGVTGGPPALVHSSALPDPNMLVSDCTASSSDLGSAIDKIIESTIGPDLIQNCITVTSAEDGGAETTRYLILQGPDDGAPMTSPMSSSTLAHSLAAIEALADGPTSTSTCLEAQGGPSSPVQLPPASGAEEPDLQSLEAMMEVVVVQQFKCKMCQYRSSTKATLLRHMRERHFRPAAAAAAAGKKGRLRKWSTSTKSQEEEGPEEEDDDDIVDAGAIDDLEEDSDYNPAEDEPRGRQLRLQRPTPSTPRPRRRPGRPRKLPRLEISDLPDGVEGEPLVSSQSGQSPPEPQDPEAPSSSGPGHLVAMGKVSRTPVEAGVSQSDAENAAPSCPDEHDTLPRRRGRPSRRFLGKKYRKYYYKSPKPLLRPFLCRICGSRFLSHEDLRFHVNSHEAGDPQLFKCLQCSYRSRRWSSLKEHMFNHVGSKPYKCDECSYTSVYRKDVIRHAAVHSRDRKKRPDPTPKLSSFPCPVCGRVYPMQKRLTQHMKTHSTEKPHMCDKCGKSFKKRYTFKMHLLTHIQAVANRRFKCEFCEFVCEDKKALLNHQLSHVSDKPFKCSFCPYRTFREDFLLSHVAVKHTGAKPFACEYCHFSTRHKKNLRLHVRCRHASSFEEWGRRHPEEPPSRRRPFFSLQQIEELKQQHSAAPGPPPSSPGPPEIPPEATTFQSSEAPSLLCSDTLGGATIIYQQGAEESTAMATQTALDLLLNMSAQRELGGTALQVAVVKSEDVEAGLASPGGQPSPEGATPQVVTLHVAEPGGGAAAESQLGPPDLPQITLAPGPFGGTGYSVITAPPMEEGTSAPGTPYSEEPAGEAAQAVVVSDTLKEAGTHYIMATDGTQLHHIELTADGSISFPSPDALASGAKWPLLQCGGLPRDGPEPPSPAKTHCVGDSQSSASSPPATSKALGLAVPPSPPSAATAASKKFSCKICAEAFPGRAEMESHKRAHAGPGAFKCPDCPFSARQWPEVRAHMAQHSSLRPHQCSQCSFASKNKKDLRRHMLTHTKEKPFACHLCGQRFNRNGHLKFHIQRLHSPDGRKSGTPTARAPTQTPTQTIILNSDDETLATLHTALQSSHGVLGPERLQQALSQEHIIVAQEQTVTNQEEAAYIQEITTADGQTVQHLVTSDNQVQYIISQDGVQHLLPQEYVVVPEGHHIQVQEGQITHIQYEQGAPFLQESQIQYVPVSPGQQLVTQAQLEAAAHSAVTAVADAAMAQAQGLFGTDETVPEHIQQLQHQGIEYDVITLADD

Target ORF information:

RefSeq Version	XM_005260504.4
Organism	Homo sapiens(human)
Definition	Homo sapiens zinc finger protein 335 (ZNF335), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005260504.4

ORF Insert Sequence:

ATGGAGGAGA ACGAGGTGGA GAGCAGCAGC GACGCGGCCC CTGGGCCTGG CCGGCCCGAG 
GAGCCCTCTG AGAGCGGCCT GGGTGTGGGC ACCTCAGAAG CCGTGTCCGC CGACAGCAGC 
GACGCCGCGG CCGCCCCGGG GCAGGCAGAG GCCGATGACT CTGGCGTGGG GCAAAGCTCG 
GACCGCGGCA GCCGTTCTCA GGAGGAGGTA TCTGAGAGCA GCTCGAGCGC AGACCCCCTG 
CCTAATAGCT ACCTCCCTGA TTCATCGTCT GTGTCTCATG GGCCAGTGGC AGGGGTGACA 
GGCGGTCCCC CAGCACTTGT GCACTCTAGT GCACTCCCAG ACCCCAACAT GCTGGTGTCC 
GACTGCACAG CTTCCTCCTC GGACCTGGGC TCGGCCATCG ACAAGATCAT CGAGTCCACC 
ATCGGGCCCG ACCTCATCCA GAACTGCATC ACTGTGACCA GTGCTGAGGA TGGCGGGGCC 
GAGACCACAC GGTACCTGAT CCTACAGGGC CCAGATGATG GAGCCCCCAT GACATCACCA 
ATGTCCAGTT CCACCTTGGC CCACAGCCTA GCAGCCATTG AGGCCCTGGC AGATGGCCCC 
ACATCCACAT CCACATGCCT GGAGGCACAG GGTGGGCCCA GCTCCCCGGT GCAGCTGCCC 
CCAGCCTCCG GTGCCGAAGA GCCGGACCTG CAGAGCCTGG AGGCCATGAT GGAGGTGGTG 
GTGGTGCAGC AGTTCAAATG CAAGATGTGC CAGTACCGGA GCAGCACCAA GGCCACACTG 
CTGCGCCACA TGCGGGAACG CCACTTCCGT CCAGCAGCAG CCGCAGCAGC AGCTGGTAAA 
AAAGGACGTC TACGGAAGTG GAGCACCTCC ACCAAGAGCC AAGAGGAAGA GGGACCAGAG 
GAGGAGGACG ATGATGACAT TGTAGACGCT GGAGCCATTG ATGACCTGGA GGAGGATAGC 
GACTATAATC CAGCTGAGGA TGAGCCCCGA GGCCGGCAGC TTCGGCTCCA GCGCCCCACC 
CCCAGTACCC CAAGGCCCCG AAGGAGACCT GGCCGGCCCC GGAAGCTGCC CCGCCTGGAG 
ATCTCAGACC TCCCAGATGG TGTGGAAGGA GAGCCTCTAG TGAGTTCCCA GAGTGGACAG 
AGCCCTCCAG AGCCACAGGA TCCCGAGGCT CCCAGCTCCT CAGGCCCAGG ACACCTGGTG 
GCCATGGGCA AGGTGAGCAG GACCCCTGTG GAAGCTGGTG TGAGCCAGTC AGATGCAGAG 
AACGCAGCCC CCTCCTGCCC GGATGAGCAT GACACTCTGC CCCGGCGCCG AGGTCGACCT 
TCCAGGCGCT TCCTAGGCAA GAAATACCGC AAGTACTATT ACAAGTCGCC CAAACCACTT 
TTGAGGCCCT TCCTGTGCCG CATCTGTGGT TCTCGCTTTC TGTCCCACGA GGACCTGCGC 
TTCCACGTCA ACTCCCATGA GGCTGGCGAT CCCCAGCTCT TCAAGTGCCT GCAGTGCAGC 
TATCGTTCCC GCCGCTGGTC CTCGCTCAAG GAGCACATGT TCAACCACGT GGGCAGCAAG 
CCCTACAAGT GTGACGAGTG CAGCTACACC AGTGTCTACC GGAAGGACGT CATTCGGCAC 
GCCGCTGTGC ACAGCCGGGA CCGGAAGAAG AGGCCAGATC CGACTCCAAA GCTGAGCTCT 
TTCCCCTGCC CTGTGTGTGG CCGTGTGTAC CCCATGCAGA AAAGACTCAC GCAGCACATG 
AAGACGCACA GCACTGAGAA GCCCCACATG TGTGACAAGT GTGGAAAGTC CTTTAAGAAG 
CGCTACACCT TCAAAATGCA CCTGCTCACG CACATCCAGG CTGTTGCCAA CCGCAGGTTC 
AAGTGTGAGT TCTGTGAGTT TGTTTGTGAA GACAAGAAGG CACTGCTGAA CCACCAGTTG 
TCCCACGTCA GTGACAAGCC CTTCAAATGC AGCTTTTGTC CCTACCGCAC CTTCCGAGAG 
GACTTCTTGC TGTCCCATGT GGCTGTCAAG CACACAGGGG CCAAGCCCTT CGCCTGTGAG 
TACTGCCACT TCAGCACACG GCACAAGAAG AACCTGCGCC TGCACGTACG GTGCCGACAC 
GCAAGCAGCT TCGAGGAATG GGGGAGGCGC CACCCTGAGG AGCCCCCCTC CCGCCGTCGC 
CCCTTCTTCT CTCTGCAGCA GATTGAGGAG CTGAAGCAGC AGCACAGTGC GGCCCCTGGA 
CCACCTCCCA GTTCCCCAGG ACCTCCTGAG ATACCCCCAG AGGCGACAAC TTTCCAGTCA 
TCTGAGGCTC CCTCATTGCT CTGTTCTGAC ACCCTGGGCG GCGCCACCAT CATCTACCAG 
CAAGGAGCTG AGGAGTCGAC AGCGATGGCC ACGCAGACAG CCTTGGATCT TCTGCTGAAC 
ATGAGTGCTC AGCGGGAACT GGGGGGCACA GCCCTGCAGG TGGCTGTGGT GAAGTCGGAA 
GATGTGGAAG CAGGGTTAGC ATCCCCTGGT GGGCAGCCCT CCCCTGAAGG TGCCACTCCA 
CAGGTGGTCA CCCTCCACGT GGCAGAGCCA GGGGGCGGTG CAGCAGCCGA GAGCCAGCTA 
GGCCCTCCTG ACCTACCGCA GATCACCCTG GCACCTGGTC CATTTGGTGG GACTGGCTAC 
AGTGTCATCA CAGCACCCCC TATGGAGGAG GGAACATCAG CTCCTGGCAC ACCTTACAGC 
GAGGAGCCCG CAGGAGAGGC AGCCCAGGCT GTGGTTGTGA GTGACACCCT AAAAGAAGCT 
GGCACCCACT ACATCATGGC TACTGATGGT ACCCAGTTGC ACCACATTGA GCTCACCGCA 
GATGGCTCCA TCTCCTTCCC AAGTCCAGAT GCTCTGGCCT CTGGTGCCAA ATGGCCCCTG 
CTGCAGTGTG GGGGACTGCC CAGAGACGGC CCTGAGCCCC CATCTCCAGC CAAGACCCAC 
TGCGTAGGGG ACTCCCAGAG CTCTGCCTCC TCACCTCCTG CAACCAGCAA AGCCCTGGGC 
CTGGCAGTGC CCCCGTCACC GCCATCTGCA GCCACTGCTG CATCAAAGAA GTTTTCCTGC 
AAGATCTGTG CCGAGGCCTT CCCTGGCCGA GCTGAGATGG AGAGTCACAA GCGGGCCCAC 
GCTGGGCCTG GTGCCTTCAA GTGCCCCGAC TGCCCCTTCA GTGCCCGCCA GTGGCCCGAG 
GTCCGGGCGC ACATGGCACA GCACTCAAGC CTACGGCCCC ACCAGTGTAG CCAGTGCAGC 
TTTGCCTCCA AGAACAAGAA GGACCTGCGT CGGCACATGC TGACTCACAC AAAGGAGAAG 
CCTTTTGCAT GCCACCTCTG CGGGCAGCGT TTCAACCGTA ACGGGCACCT CAAGTTCCAC 
ATCCAGCGGC TGCACAGTCC TGATGGGAGG AAGTCAGGAA CCCCTACAGC CCGGGCCCCT 
ACCCAGACCC CAACCCAGAC CATCATCCTG AACAGTGATG ACGAAACACT GGCCACCCTG 
CACACTGCAC TCCAGTCCAG TCACGGGGTC CTGGGCCCAG AGCGGCTACA GCAGGCACTG 
AGCCAGGAAC ACATCATCGT TGCCCAGGAA CAGACAGTGA CCAATCAGGA GGAAGCCGCC 
TACATCCAAG AGATCACCAC GGCAGATGGC CAGACCGTAC AGCACCTGGT GACCTCCGAC 
AACCAGGTGC AGTATATCAT CTCCCAGGAT GGTGTCCAGC ACCTGCTCCC CCAGGAATAT 
GTTGTGGTCC CTGAAGGCCA TCACATCCAG GTACAGGAGG GCCAGATCAC ACACATCCAG 
TATGAACAAG GAGCCCCGTT CCTTCAGGAG TCCCAGATCC AGTATGTGCC TGTGTCCCCA 
GGCCAGCAGC TTGTCACACA GGCTCAACTT GAGGCTGCAG CACACTCAGC TGTCACAGCA 
GTGGCTGATG CTGCCATGGC CCAAGCCCAG GGCCTGTTTG GTACAGACGA GACAGTGCCC 
GAACACATTC AACAGCTGCA GCACCAGGGC ATCGAGTACG ACGTCATCAC CCTGGCCGAT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Association Between Invisible Basal Ganglia and ZNF335 Mutations: A Case Report.
Pediatrics138(3)(2016 Sep)
Sato R,Takanashi J,Tsuyusaki Y,Kato M,Saitsu H,Matsumoto N,Takahashi T

Anaesthesia and orphan disease: primary autosomal recessive microcephaly-10 caused by a mutation in the ZNF335 gene.
European journal of anaesthesiology33(7)543-5(2016 07)
Nishida T,Mihara T,Horiki T,Ka K

Common polygenic variation in coeliac disease and confirmation of ZNF335 and NIFA as disease susceptibility loci.
European journal of human genetics : EJHG24(2)291-7(2016 Feb)
Coleman C,Quinn EM,Ryan AW,Conroy J,Trimble V,Mahmud N,Kennedy N,Corvin AP,Morris DW,Donohoe G,O'Morain C,MacMathuna P,Byrnes V,Kiat C,Trynka G,Wijmenga C,Kelleher D,Ennis S,Anney RJ,McManus R

IMAC fractionation in combination with LC-MS reveals H2B and NIF-1 peptides as potential bladder cancer biomarkers.
Journal of proteome research12(9)3969-79(2013 Sep)
Frantzi M,Zoidakis J,Papadopoulos T,Z?rbig P,Katafigiotis I,Stravodimos K,Lazaris A,Giannopoulou I,Ploumidis A,Mischak H,Mullen W,Vlahou A

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ZNF335 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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