SDHB cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	SDHB
Entrez Gene ID	6390
Full Name	succinate dehydrogenase complex iron sulfur subunit B
Synonyms	CWS2,IP,PGL4,SDH,SDH1,SDH2,SDHIP
General protein information	Preferred Names succinate dehydrogenase complex iron sulfur subunit B Names succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial iron-sulfur subunit of complex II succinate dehydrogenase complex, subunit B, iron sulfur (Ip)
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1p36.13
Summary	Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq, Jul 2008].
Disorder MIM:	185470
Disorder Html:	Paragangliomas 4, 115310 (3); Pheochromocytoma, 171300 (3); Paraganglioma and gastric stromal sarcoma, 606864 (3); Cowden syndrome 2, 612359 (3); Gastrointestinal stromal tumor, 606764 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_003000.3	NP_002991.2	succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial precursor
NM_003000.2	NP_002991.2	succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial precursor

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007309	XP_308512.4
Kluyveromyces lactis	KLLA0E00683g	XP_453977.1
	SDHB	XP_001088349.1
Saccharomyces cerevisiae (baker's yeast)	SDH2	NP_013059.1
Eremothecium gossypii	AGOS_ACL065C	NP_983339.1
Neurospora crassa	NCU00959	XP_965152.1
Bos taurus (cattle)	SDHB	NP_001035573.1
Homo sapiens (human)	SDHB	NP_002991.2
Mus musculus (house mouse)	Sdhb	NP_075863.2
Rattus norvegicus (Norway rat)	Sdhb	NP_001094009.1
Gallus gallus (chicken)	SDHB	XP_004950147.1
Danio rerio (zebrafish)	sdhb	NP_001092210.1
Drosophila melanogaster (fruit fly)	SdhB	NP_477101.1
Arabidopsis thaliana (thale cress)	SDH2-1	NP_001118718.1
Arabidopsis thaliana (thale cress)	SDH2-2	NP_198881.1
Oryza sativa (rice)	Os08g0120000	NP_001060876.1
Xenopus tropicalis (tropical clawed frog)	sdhb	NP_001120000.1
Pan troglodytes (chimpanzee)	SDHB	XP_003949355.1
Canis lupus familiaris (dog)	SDHB	NP_001239146.1
Caenorhabditis elegans (roundworm)	sdhb-1	NP_495992.1
Schizosaccharomyces pombe (fission yeast)	sdh2	NP_593530.2
Magnaporthe oryzae (rice blast fungus)	MGG_00167	XP_003718958.1

Gene Ontology

The following SDHB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SDHB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18105	NM_003000.3 Latest version!	Homo sapiens succinate dehydrogenase complex iron sulfur subunit B (SDHB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
OHu18105	NM_003000.2	Homo sapiens succinate dehydrogenase complex iron sulfur subunit B (SDHB), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$216.30 $309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SDHB ( NM_003000.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_002991.2 Homo sapiens succinate dehydrogenase complex iron sulfur subunit B (SDHB), mRNA.

Price & Availability↑

CloneID	OHu18105
Clone ID Related Accession (Same CDS sequence)	NM_003000.3 , NM_003000.2
Accession Version	NM_003000.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC007840.2, BF796275.1 and BU554163.1. This sequence is a reference standard in the RefSeqGene project. On Sep 28, 2006 this sequence version replaced NM_003000.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.327377.1, U17248.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2154361 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 2302193; reported by MitoCarta RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TGGTCGCCCT CTCCTTGAGG CGCCGGTTGC CGGCCACAAC CCTTGGCGGA 
GCCTGCCTGC AGGCCTCCCG AGGAGCCCAG ACAGCTGCAG CCACAGCTCC CCGTATCAAG 
AAATTTGCCA TCTATCGATG GGACCCAGAC AAGGCTGGAG ACAAACCTCA TATGCAGACT 
TATGAAGTTG ACCTTAATAA ATGTGGCCCC ATGGTATTGG ATGCTTTAAT CAAGATTAAG 
AATGAAGTTG ACTCTACTTT GACCTTCCGA AGATCATGCA GAGAAGGCAT CTGTGGCTCT 
TGTGCAATGA ACATCAATGG AGGCAACACT CTAGCTTGCA CCCGAAGGAT TGACACCAAC 
CTCAATAAGG TCTCAAAAAT CTACCCTCTT CCACACATGT ATGTGATAAA GGATCTTGTT 
CCCGATTTGA GCAACTTCTA TGCACAGTAC AAATCCATTG AGCCTTATTT GAAGAAGAAG 
GATGAATCTC AGGAAGGCAA GCAGCAGTAT CTGCAGTCCA TAGAAGAGCG TGAGAAACTG 
GACGGGCTCT ACGAGTGCAT TCTCTGTGCC TGCTGTAGCA CCAGCTGCCC CAGCTACTGG 
TGGAACGGAG ACAAATATCT GGGGCCTGCA GTTCTTATGC AGGCCTATCG CTGGATGATT 
GACTCCAGAG ATGACTTCAC AGAGGAGCGC CTGGCCAAGC TGCAGGACCC ATTCTCTCTA 
TACCGCTGCC ACACCATCAT GAACTGCACA AGGACCTGTC CTAAGGGTCT GAATCCAGGG 
AAAGCTATTG CAGAGATCAA GAAAATGATG GCAACCTATA AGGAGAAGAA AGCTTCAGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002991.2
CDS	152..994
Translation	MAAVVALSLRRRLPATTLGGACLQASRGAQTAAATAPRIKKFAIYRWDPDKAGDKPHMQTYEVDLNKCGPMVLDALIKIKNEVDSTLTFRRSCREGICGSCAMNINGGNTLACTRRIDTNLNKVSKIYPLPHMYVIKDLVPDLSNFYAQYKSIEPYLKKKDESQEGKQQYLQSIEEREKLDGLYECILCACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDFTEERLAKLQDPFSLYRCHTIMNCTRTCPKGLNPGKAIAEIKKMMATYKEKKASV

Target ORF information:

RefSeq Version	NM_003000.2
Organism	Homo sapiens(human)
Definition	Homo sapiens succinate dehydrogenase complex iron sulfur subunit B (SDHB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003000.2

ORF Insert Sequence:

ATGGCGGCGG TGGTCGCCCT CTCCTTGAGG CGCCGGTTGC CGGCCACAAC CCTTGGCGGA 
GCCTGCCTGC AGGCCTCCCG AGGAGCCCAG ACAGCTGCAG CCACAGCTCC CCGTATCAAG 
AAATTTGCCA TCTATCGATG GGACCCAGAC AAGGCTGGAG ACAAACCTCA TATGCAGACT 
TATGAAGTTG ACCTTAATAA ATGTGGCCCC ATGGTATTGG ATGCTTTAAT CAAGATTAAG 
AATGAAGTTG ACTCTACTTT GACCTTCCGA AGATCATGCA GAGAAGGCAT CTGTGGCTCT 
TGTGCAATGA ACATCAATGG AGGCAACACT CTAGCTTGCA CCCGAAGGAT TGACACCAAC 
CTCAATAAGG TCTCAAAAAT CTACCCTCTT CCACACATGT ATGTGATAAA GGATCTTGTT 
CCCGATTTGA GCAACTTCTA TGCACAGTAC AAATCCATTG AGCCTTATTT GAAGAAGAAG 
GATGAATCTC AGGAAGGCAA GCAGCAGTAT CTGCAGTCCA TAGAAGAGCG TGAGAAACTG 
GACGGGCTCT ACGAGTGCAT TCTCTGTGCC TGCTGTAGCA CCAGCTGCCC CAGCTACTGG 
TGGAACGGAG ACAAATATCT GGGGCCTGCA GTTCTTATGC AGGCCTATCG CTGGATGATT 
GACTCCAGAG ATGACTTCAC AGAGGAGCGC CTGGCCAAGC TGCAGGACCC ATTCTCTCTA 
TACCGCTGCC ACACCATCAT GAACTGCACA AGGACCTGTC CTAAGGGTCT GAATCCAGGG 
AAAGCTATTG CAGAGATCAA GAAAATGATG GCAACCTATA AGGAGAAGAA AGCTTCAGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SDHB ( NM_003000.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002991.2 Homo sapiens succinate dehydrogenase complex iron sulfur subunit B (SDHB), mRNA.

Price & Availability↑

CloneID	OHu18105
Clone ID Related Accession (Same CDS sequence)	NM_003000.3 , NM_003000.2
Accession Version	NM_003000.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 843bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-14
Organism	Homo sapiens(human)
Product	succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC007840.2, BF796275.1 and BU554163.1. On Aug 31, 2019 this sequence version replaced NM_003000.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK312056.1, BM923277.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: PMID: 2302193; reported by MitoCarta RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCGGCGG TGGTCGCCCT CTCCTTGAGG CGCCGGTTGC CGGCCACAAC CCTTGGCGGA 
GCCTGCCTGC AGGCCTCCCG AGGAGCCCAG ACAGCTGCAG CCACAGCTCC CCGTATCAAG 
AAATTTGCCA TCTATCGATG GGACCCAGAC AAGGCTGGAG ACAAACCTCA TATGCAGACT 
TATGAAGTTG ACCTTAATAA ATGTGGCCCC ATGGTATTGG ATGCTTTAAT CAAGATTAAG 
AATGAAGTTG ACTCTACTTT GACCTTCCGA AGATCATGCA GAGAAGGCAT CTGTGGCTCT 
TGTGCAATGA ACATCAATGG AGGCAACACT CTAGCTTGCA CCCGAAGGAT TGACACCAAC 
CTCAATAAGG TCTCAAAAAT CTACCCTCTT CCACACATGT ATGTGATAAA GGATCTTGTT 
CCCGATTTGA GCAACTTCTA TGCACAGTAC AAATCCATTG AGCCTTATTT GAAGAAGAAG 
GATGAATCTC AGGAAGGCAA GCAGCAGTAT CTGCAGTCCA TAGAAGAGCG TGAGAAACTG 
GACGGGCTCT ACGAGTGCAT TCTCTGTGCC TGCTGTAGCA CCAGCTGCCC CAGCTACTGG 
TGGAACGGAG ACAAATATCT GGGGCCTGCA GTTCTTATGC AGGCCTATCG CTGGATGATT 
GACTCCAGAG ATGACTTCAC AGAGGAGCGC CTGGCCAAGC TGCAGGACCC ATTCTCTCTA 
TACCGCTGCC ACACCATCAT GAACTGCACA AGGACCTGTC CTAAGGGTCT GAATCCAGGG 
AAAGCTATTG CAGAGATCAA GAAAATGATG GCAACCTATA AGGAGAAGAA AGCTTCAGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002991.2
CDS	14..856
Translation	MAAVVALSLRRRLPATTLGGACLQASRGAQTAAATAPRIKKFAIYRWDPDKAGDKPHMQTYEVDLNKCGPMVLDALIKIKNEVDSTLTFRRSCREGICGSCAMNINGGNTLACTRRIDTNLNKVSKIYPLPHMYVIKDLVPDLSNFYAQYKSIEPYLKKKDESQEGKQQYLQSIEEREKLDGLYECILCACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDFTEERLAKLQDPFSLYRCHTIMNCTRTCPKGLNPGKAIAEIKKMMATYKEKKASV

Target ORF information:

RefSeq Version	NM_003000.3
Organism	Homo sapiens(human)
Definition	Homo sapiens succinate dehydrogenase complex iron sulfur subunit B (SDHB), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_003000.3

ORF Insert Sequence:

ATGGCGGCGG TGGTCGCCCT CTCCTTGAGG CGCCGGTTGC CGGCCACAAC CCTTGGCGGA 
GCCTGCCTGC AGGCCTCCCG AGGAGCCCAG ACAGCTGCAG CCACAGCTCC CCGTATCAAG 
AAATTTGCCA TCTATCGATG GGACCCAGAC AAGGCTGGAG ACAAACCTCA TATGCAGACT 
TATGAAGTTG ACCTTAATAA ATGTGGCCCC ATGGTATTGG ATGCTTTAAT CAAGATTAAG 
AATGAAGTTG ACTCTACTTT GACCTTCCGA AGATCATGCA GAGAAGGCAT CTGTGGCTCT 
TGTGCAATGA ACATCAATGG AGGCAACACT CTAGCTTGCA CCCGAAGGAT TGACACCAAC 
CTCAATAAGG TCTCAAAAAT CTACCCTCTT CCACACATGT ATGTGATAAA GGATCTTGTT 
CCCGATTTGA GCAACTTCTA TGCACAGTAC AAATCCATTG AGCCTTATTT GAAGAAGAAG 
GATGAATCTC AGGAAGGCAA GCAGCAGTAT CTGCAGTCCA TAGAAGAGCG TGAGAAACTG 
GACGGGCTCT ACGAGTGCAT TCTCTGTGCC TGCTGTAGCA CCAGCTGCCC CAGCTACTGG 
TGGAACGGAG ACAAATATCT GGGGCCTGCA GTTCTTATGC AGGCCTATCG CTGGATGATT 
GACTCCAGAG ATGACTTCAC AGAGGAGCGC CTGGCCAAGC TGCAGGACCC ATTCTCTCTA 
TACCGCTGCC ACACCATCAT GAACTGCACA AGGACCTGTC CTAAGGGTCT GAATCCAGGG 
AAAGCTATTG CAGAGATCAA GAAAATGATG GCAACCTATA AGGAGAAGAA AGCTTCAGTT 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.