RPL35A cDNA ORF clone, Homo sapiens(Human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPL35A
Entrez Gene ID	6165
Full Name	ribosomal protein L35a
Synonyms	DBA5,L35A
General protein information	Preferred Names ribosomal protein L35a Names 60S ribosomal protein L35a cell growth-inhibiting gene 33 protein large ribosomal subunit protein eL33
Gene Type	protein-coding
Organism	Homo sapiens(Human)
Genome	Chromosome: 3 Map Location: 3q29
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L35AE family of ribosomal proteins. It is located in the cytoplasm. The rat protein has been shown to bind to both initiator and elongator tRNAs, and thus, it is located at the P site, or P and A sites, of the ribosome. Although this gene was originally mapped to chromosome 18, it has been established that it is located at 3q29-qter. Alternative splicing results in multiple transcript variants. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Oct 2015].
Disorder MIM:	180468
Disorder Html:	Diamond-Blackfan anemia 5, 612528 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001316311.2	NP_001303240.1	60S ribosomal protein L35a
XM_005269351.1	XP_005269408.1	60S ribosomal protein L35a isoform X1
NM_000996.4	NP_000987.2	60S ribosomal protein L35a
NM_001316311.1	NP_001303240.1	60S ribosomal protein L35a
NM_000996.3	NP_000987.2	60S ribosomal protein L35a
NM_000996.2	NP_000987.2	60S ribosomal protein L35a

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Rpl35a	NP_067313.2
Rattus norvegicus (Norway rat)	LOC100359498	XP_002727942.1
Homo sapiens (human)	RPL35A	NP_000987.2
	LOC711011	XP_001110581.2
Rattus norvegicus (Norway rat)	Rpl35a	NP_067087.1
Danio rerio (zebrafish)	rpl35a	NP_001002487.1
Drosophila melanogaster (fruit fly)	RpL35A	NP_649539.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP002754	XP_312171.3
Caenorhabditis elegans (roundworm)	rpl-33	NP_495468.1
Schizosaccharomyces pombe (fission yeast)	rpl35b	NP_001018813.2
Pan troglodytes (chimpanzee)	RPL35A	NP_001238881.1
Bos taurus (cattle)	RPL35A	NP_001020492.1
Gallus gallus (chicken)	RPL35A	NP_001264386.1
Arabidopsis thaliana (thale cress)	AT3G55750	NP_191134.1
Arabidopsis thaliana (thale cress)	AT1G07070	NP_172188.1
Oryza sativa (rice)	Os05g0556900	NP_001056284.1
	LOC700960	XP_001094991.2
	RPL35A	XP_001103775.1
	LOC715460	XP_001112591.2
Canis lupus familiaris (dog)	RPL35A	XP_535773.1
Schizosaccharomyces pombe (fission yeast)	rpl35a	NP_587864.1
Neurospora crassa	NCU09109	XP_958318.2
Oryza sativa (rice)	Os02g0785800	NP_001048330.1
Xenopus tropicalis (tropical clawed frog)	rpl35a	NP_001005134.1
Saccharomyces cerevisiae (baker's yeast)	RPL33B	NP_014877.3
Saccharomyces cerevisiae (baker's yeast)	RPL33A	NP_015182.1
Kluyveromyces lactis	KLLA0D07405g	XP_453392.1
Eremothecium gossypii	AGOS_ACR120C	NP_983522.1
Magnaporthe oryzae (rice blast fungus)	MGG_09927	XP_003710027.1
Arabidopsis thaliana (thale cress)	AT1G41880	NP_174951.1
Arabidopsis thaliana (thale cress)	AT1G74270	NP_177567.1

Gene Ontology
Bibliography

Related articles in PubMed

Novel 3q27.2-qter deletion in a patient with Diamond-Blackfan anemia and immunodeficiency: Case report and review of literature.
Alkhunaizi E, Schrewe B, Alizadehfar R, V?zina C, Stewart GS, Braverman N
American journal of medical genetics. Part A173(6)1514-1520(2017 Jun)

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Jefferson M, Donaszi-Ivanov A, Pollen S, Dalmay T, Saalbach G, Powell PP
Journal of virology88(18)10340-53(2014 Sep)

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Zanivan S, Maione F, Hein MY, Hern?ndez-Fernaud JR, Ostasiewicz P, Giraudo E, Mann M
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We identified 85 overlapping deletions, of which six included the RPL35A gene and all should be had Diamond-Blackfan anemia (DBA).we sequenced the remaining RNF168 gene and examined her fibroblast culture for a DNA double strand break repair deficiency. These results were normal, indicating that the immunodeficiency is unlikely to result from a RNF168 deficiency.
Title: Novel 3q27.2-qter deletion in a patient with Diamond-Blackfan anemia and immunodeficiency: Case report and review of literature.

Data show 1 proband with an RPL5 deletion, 1 patient with an RPL35A deletion, 3 with RPS17 deletions, and 1 with an RPS19 deletion.
Title: Extensive gene deletions in Japanese patients with Diamond-Blackfan anemia.

Studies identified deletions at known Diamond-Blackfan anemia (DBA)-related ribosomal protein gene loci in 17% (9 of 51) of patients without an identifiable mutation, including RPS19, RPS17, RPS26, and RPL35A.
Title: Ribosomal protein gene deletions in Diamond-Blackfan anemia.

Observational study of gene-disease association. (HuGE Navigator)
Title: Genetic variants in nuclear-encoded mitochondrial genes influence AIDS progression.

Observational study of gene-disease association. (HuGE Navigator)
Title: Mutations in the ribosomal protein genes in Japanese patients with Diamond-Blackfan anemia.

The following RPL35A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPL35A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19419	NM_000996.4 Latest version!	Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu19419	NM_001316311.2 Latest version!	Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu19419	XM_005269351.1 Latest version!	Homo sapiens ribosomal protein L35a (RPL35A), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu19419	NM_001316311.1	Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu19419	NM_000996.3	Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu19419	NM_000996.2	Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPL35A ( NM_001316311.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001303240.1 Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19419
Clone ID Related Accession (Same CDS sequence)	NM_001316311.2 , XM_005269351.1 , NM_000996.4 , NM_001316311.1 , NM_000996.3 , NM_000996.2
Accession Version	NM_001316311.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	60S ribosomal protein L35a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC135893.2 and BC017093.1. On May 31, 2019 this sequence version replaced NM_001316311.1. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DB279260.1, BC017093.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGAA GGCTGTGGTC CAAGGCCATT TTTGCTGGCT ATAAGCGGGG TCTCCGGAAC 
CAAAGGGAGC ACACAGCTCT TCTTAAAATT GAAGGTGTTT ACGCCCGAGA TGAAACAGAA 
TTCTATTTGG GCAAGAGATG CGCTTATGTA TATAAAGCAA AGAACAACAC AGTCACTCCT 
GGCGGCAAAC CAAACAAAAC CAGAGTCATC TGGGGAAAAG TAACTCGGGC CCATGGAAAC 
AGTGGCATGG TTCGTGCCAA ATTCCGAAGC AATCTTCCTG CTAAGGCCAT TGGACACAGA 
ATCCGAGTGA TGCTGTACCC CTCAAGGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303240.1
CDS	143..475
Translation	MSGRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPGGKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI

Target ORF information:

RefSeq Version	NM_001316311.2
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316311.2

ORF Insert Sequence:

ATGTCTGGAA GGCTGTGGTC CAAGGCCATT TTTGCTGGCT ATAAGCGGGG TCTCCGGAAC 
CAAAGGGAGC ACACAGCTCT TCTTAAAATT GAAGGTGTTT ACGCCCGAGA TGAAACAGAA 
TTCTATTTGG GCAAGAGATG CGCTTATGTA TATAAAGCAA AGAACAACAC AGTCACTCCT 
GGCGGCAAAC CAAACAAAAC CAGAGTCATC TGGGGAAAAG TAACTCGGGC CCATGGAAAC 
AGTGGCATGG TTCGTGCCAA ATTCCGAAGC AATCTTCCTG CTAAGGCCAT TGGACACAGA 
ATCCGAGTGA TGCTGTACCC CTCAAGGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL35A ( NM_000996.4 ) cDNA ORF clone, Homo sapiens(Human) -> NP_000987.2 Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu19419
Clone ID Related Accession (Same CDS sequence)	NM_001316311.2 , XM_005269351.1 , NM_000996.4 , NM_001316311.1 , NM_000996.3 , NM_000996.2
Accession Version	NM_000996.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	60S ribosomal protein L35a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BC010949.1, BM972808.1 and AW575227.1. This sequence is a reference standard in the RefSeqGene project. On May 8, 2018 this sequence version replaced NM_000996.3. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189658.66844.1, SRR5189658.88288.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000647248.2/ ENSP00000495672.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTGGAA GGCTGTGGTC CAAGGCCATT TTTGCTGGCT ATAAGCGGGG TCTCCGGAAC 
CAAAGGGAGC ACACAGCTCT TCTTAAAATT GAAGGTGTTT ACGCCCGAGA TGAAACAGAA 
TTCTATTTGG GCAAGAGATG CGCTTATGTA TATAAAGCAA AGAACAACAC AGTCACTCCT 
GGCGGCAAAC CAAACAAAAC CAGAGTCATC TGGGGAAAAG TAACTCGGGC CCATGGAAAC 
AGTGGCATGG TTCGTGCCAA ATTCCGAAGC AATCTTCCTG CTAAGGCCAT TGGACACAGA 
ATCCGAGTGA TGCTGTACCC CTCAAGGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000987.2
CDS	65..397
Translation	MSGRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPGGKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI

Target ORF information:

RefSeq Version	NM_000996.4
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000996.4

ORF Insert Sequence:

ATGTCTGGAA GGCTGTGGTC CAAGGCCATT TTTGCTGGCT ATAAGCGGGG TCTCCGGAAC 
CAAAGGGAGC ACACAGCTCT TCTTAAAATT GAAGGTGTTT ACGCCCGAGA TGAAACAGAA 
TTCTATTTGG GCAAGAGATG CGCTTATGTA TATAAAGCAA AGAACAACAC AGTCACTCCT 
GGCGGCAAAC CAAACAAAAC CAGAGTCATC TGGGGAAAAG TAACTCGGGC CCATGGAAAC 
AGTGGCATGG TTCGTGCCAA ATTCCGAAGC AATCTTCCTG CTAAGGCCAT TGGACACAGA 
ATCCGAGTGA TGCTGTACCC CTCAAGGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL35A ( NM_001316311.1 ) cDNA ORF clone, Homo sapiens(Human) -> NP_001303240.1 Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19419
Clone ID Related Accession (Same CDS sequence)	NM_001316311.2 , XM_005269351.1 , NM_000996.4 , NM_001316311.1 , NM_000996.3 , NM_000996.2
Accession Version	NM_001316311.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	60S ribosomal protein L35a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC017093.1. On Oct 9, 2015 this sequence version replaced XM_005269351.1. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017093.1, SRR1163657.455398.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTGGAA GGCTGTGGTC CAAGGCCATT TTTGCTGGCT ATAAGCGGGG TCTCCGGAAC 
CAAAGGGAGC ACACAGCTCT TCTTAAAATT GAAGGTGTTT ACGCCCGAGA TGAAACAGAA 
TTCTATTTGG GCAAGAGATG CGCTTATGTA TATAAAGCAA AGAACAACAC AGTCACTCCT 
GGCGGCAAAC CAAACAAAAC CAGAGTCATC TGGGGAAAAG TAACTCGGGC CCATGGAAAC 
AGTGGCATGG TTCGTGCCAA ATTCCGAAGC AATCTTCCTG CTAAGGCCAT TGGACACAGA 
ATCCGAGTGA TGCTGTACCC CTCAAGGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001303240.1
CDS	109..441
Translation	MSGRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPGGKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI

Target ORF information:

RefSeq Version	NM_001316311.1
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001316311.1

ORF Insert Sequence:

ATGTCTGGAA GGCTGTGGTC CAAGGCCATT TTTGCTGGCT ATAAGCGGGG TCTCCGGAAC 
CAAAGGGAGC ACACAGCTCT TCTTAAAATT GAAGGTGTTT ACGCCCGAGA TGAAACAGAA 
TTCTATTTGG GCAAGAGATG CGCTTATGTA TATAAAGCAA AGAACAACAC AGTCACTCCT 
GGCGGCAAAC CAAACAAAAC CAGAGTCATC TGGGGAAAAG TAACTCGGGC CCATGGAAAC 
AGTGGCATGG TTCGTGCCAA ATTCCGAAGC AATCTTCCTG CTAAGGCCAT TGGACACAGA 
ATCCGAGTGA TGCTGTACCC CTCAAGGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL35A ( NM_000996.3 ) cDNA ORF clone, Homo sapiens(Human) -> NP_000987.2 Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu19419
Clone ID Related Accession (Same CDS sequence)	NM_001316311.2 , XM_005269351.1 , NM_000996.4 , NM_001316311.1 , NM_000996.3 , NM_000996.2
Accession Version	NM_000996.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	60S ribosomal protein L35a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BC010949.1 and BC061890.1. This sequence is a reference standard in the RefSeqGene project. On Dec 19, 2017 this sequence version replaced NM_000996.2. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AU152019.1, BC010949.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGGAA GGCTGTGGTC CAAGGCCATT TTTGCTGGCT ATAAGCGGGG TCTCCGGAAC 
CAAAGGGAGC ACACAGCTCT TCTTAAAATT GAAGGTGTTT ACGCCCGAGA TGAAACAGAA 
TTCTATTTGG GCAAGAGATG CGCTTATGTA TATAAAGCAA AGAACAACAC AGTCACTCCT 
GGCGGCAAAC CAAACAAAAC CAGAGTCATC TGGGGAAAAG TAACTCGGGC CCATGGAAAC 
AGTGGCATGG TTCGTGCCAA ATTCCGAAGC AATCTTCCTG CTAAGGCCAT TGGACACAGA 
ATCCGAGTGA TGCTGTACCC CTCAAGGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000987.2
CDS	65..397
Translation	MSGRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPGGKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI

Target ORF information:

RefSeq Version	NM_000996.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000996.3

ORF Insert Sequence:

ATGTCTGGAA GGCTGTGGTC CAAGGCCATT TTTGCTGGCT ATAAGCGGGG TCTCCGGAAC 
CAAAGGGAGC ACACAGCTCT TCTTAAAATT GAAGGTGTTT ACGCCCGAGA TGAAACAGAA 
TTCTATTTGG GCAAGAGATG CGCTTATGTA TATAAAGCAA AGAACAACAC AGTCACTCCT 
GGCGGCAAAC CAAACAAAAC CAGAGTCATC TGGGGAAAAG TAACTCGGGC CCATGGAAAC 
AGTGGCATGG TTCGTGCCAA ATTCCGAAGC AATCTTCCTG CTAAGGCCAT TGGACACAGA 
ATCCGAGTGA TGCTGTACCC CTCAAGGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL35A ( NM_000996.2 ) cDNA ORF clone, Homo sapiens(Human) -> NP_000987.2 Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu19419
Clone ID Related Accession (Same CDS sequence)	NM_001316311.2 , XM_005269351.1 , NM_000996.4 , NM_001316311.1 , NM_000996.3 , NM_000996.2
Accession Version	NM_000996.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-04-24
Organism	Homo sapiens(Human)
Product	60S ribosomal protein L35a
Comment	REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from BC010949.1. This sequence is a reference standard in the RefSeqGene project. On Oct 13, 2001 this sequence version replaced NM_000996.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.622202.1, BC010949.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGTCTGGAA GGCTGTGGTC CAAGGCCATT TTTGCTGGCT ATAAGCGGGG TCTCCGGAAC 
CAAAGGGAGC ACACAGCTCT TCTTAAAATT GAAGGTGTTT ACGCCCGAGA TGAAACAGAA 
TTCTATTTGG GCAAGAGATG CGCTTATGTA TATAAAGCAA AGAACAACAC AGTCACTCCT 
GGCGGCAAAC CAAACAAAAC CAGAGTCATC TGGGGAAAAG TAACTCGGGC CCATGGAAAC 
AGTGGCATGG TTCGTGCCAA ATTCCGAAGC AATCTTCCTG CTAAGGCCAT TGGACACAGA 
ATCCGAGTGA TGCTGTACCC CTCAAGGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000987.2
CDS	74..406
Translation	MSGRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPGGKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI

Target ORF information:

RefSeq Version	NM_000996.2
Organism	Homo sapiens(Human)
Definition	Homo sapiens ribosomal protein L35a (RPL35A), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000996.2

ORF Insert Sequence:

ATGTCTGGAA GGCTGTGGTC CAAGGCCATT TTTGCTGGCT ATAAGCGGGG TCTCCGGAAC 
CAAAGGGAGC ACACAGCTCT TCTTAAAATT GAAGGTGTTT ACGCCCGAGA TGAAACAGAA 
TTCTATTTGG GCAAGAGATG CGCTTATGTA TATAAAGCAA AGAACAACAC AGTCACTCCT 
GGCGGCAAAC CAAACAAAAC CAGAGTCATC TGGGGAAAAG TAACTCGGGC CCATGGAAAC 
AGTGGCATGG TTCGTGCCAA ATTCCGAAGC AATCTTCCTG CTAAGGCCAT TGGACACAGA 
ATCCGAGTGA TGCTGTACCC CTCAAGGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RPL35A ( XM_005269351.1 ) cDNA ORF clone, Homo sapiens(Human) -> XP_005269408.1 Homo sapiens ribosomal protein L35a (RPL35A), transcript variant X1, mRNA.

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CloneID	OHu19419
Clone ID Related Accession (Same CDS sequence)	NM_001316311.2 , XM_005269351.1 , NM_000996.4 , NM_001316311.1 , NM_000996.3 , NM_000996.2
Accession Version	XM_005269351.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 333bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	60S ribosomal protein L35a isoform X1
Comment

ATGTCTGGAA GGCTGTGGTC CAAGGCCATT TTTGCTGGCT ATAAGCGGGG TCTCCGGAAC 
CAAAGGGAGC ACACAGCTCT TCTTAAAATT GAAGGTGTTT ACGCCCGAGA TGAAACAGAA 
TTCTATTTGG GCAAGAGATG CGCTTATGTA TATAAAGCAA AGAACAACAC AGTCACTCCT 
GGCGGCAAAC CAAACAAAAC CAGAGTCATC TGGGGAAAAG TAACTCGGGC CCATGGAAAC 
AGTGGCATGG TTCGTGCCAA ATTCCGAAGC AATCTTCCTG CTAAGGCCAT TGGACACAGA 
ATCCGAGTGA TGCTGTACCC CTCAAGGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005269408.1
Translation	MSGRLWSKAIFAGYKRGLRNQREHTALLKIEGVYARDETEFYLGKRCAYVYKAKNNTVTPGGKPNKTRVIWGKVTRAHGNSGMVRAKFRSNLPAKAIGHRIRVMLYPSRI

Target ORF information:

RefSeq Version	XM_005269351.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens ribosomal protein L35a (RPL35A), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005269351.1

ORF Insert Sequence:

ATGTCTGGAA GGCTGTGGTC CAAGGCCATT TTTGCTGGCT ATAAGCGGGG TCTCCGGAAC 
CAAAGGGAGC ACACAGCTCT TCTTAAAATT GAAGGTGTTT ACGCCCGAGA TGAAACAGAA 
TTCTATTTGG GCAAGAGATG CGCTTATGTA TATAAAGCAA AGAACAACAC AGTCACTCCT 
GGCGGCAAAC CAAACAAAAC CAGAGTCATC TGGGGAAAAG TAACTCGGGC CCATGGAAAC 
AGTGGCATGG TTCGTGCCAA ATTCCGAAGC AATCTTCCTG CTAAGGCCAT TGGACACAGA 
ATCCGAGTGA TGCTGTACCC CTCAAGGATT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel 3q27.2-qter deletion in a patient with Diamond-Blackfan anemia and immunodeficiency: Case report and review of literature.
American journal of medical genetics. Part A173(6)1514-1520(2017 Jun)
Alkhunaizi E,Schrewe B,Alizadehfar R,V?zina C,Stewart GS,Braverman N

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Journal of virology88(18)10340-53(2014 Sep)
Jefferson M,Donaszi-Ivanov A,Pollen S,Dalmay T,Saalbach G,Powell PP

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)
Zanivan S,Maione F,Hein MY,Hern?ndez-Fernaud JR,Ostasiewicz P,Giraudo E,Mann M

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

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RPL35A cDNA ORF clone, Homo sapiens(Human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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