RPL21 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RPL21
Entrez Gene ID	6144
Full Name	ribosomal protein L21
Synonyms	HYPT12,L21
General protein information	Preferred Names ribosomal protein L21 Names 60S ribosomal protein L21 large ribosomal subunit protein eL21
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 13 Map Location: 13q12.2
Summary	Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L21E family of ribosomal proteins. It is located in the cytoplasm. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq, Jul 2008].
Disorder MIM:	603636
Disorder Html:	Hypotrichosis 12, 615885 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_000982.3	NP_000973.2	60S ribosomal protein L21

Pathways from BioSystems

Homologies

Homo sapiens (human)	RPL21	NP_000973.2
Bos taurus (cattle)	RPL21	NP_001178341.1
Rattus norvegicus (Norway rat)	Rpl21	NP_445782.1
Caenorhabditis elegans (roundworm)	rpl-21	NP_498774.1
Saccharomyces cerevisiae (baker's yeast)	RPL21B	NP_015246.1
Magnaporthe oryzae (rice blast fungus)	MGG_02953	XP_003720754.1
	LOC703876	XP_001094881.1
Gallus gallus (chicken)	RPL21	XP_001233393.1
Schizosaccharomyces pombe (fission yeast)	rpl2102	NP_594175.1
Mus musculus (house mouse)	Rpl21	NP_062621.2
Drosophila melanogaster (fruit fly)	RpL21	NP_001260689.1
Kluyveromyces lactis	KLLA0E23651g	XP_455019.1
Neurospora crassa	NCU01948	XP_964232.1
Arabidopsis thaliana (thale cress)	AT1G09590	NP_563847.1
Arabidopsis thaliana (thale cress)	AT1G09690	NP_563849.1
Oryza sativa (rice)	Os10g0465800	NP_001064799.1
Xenopus tropicalis (tropical clawed frog)	rpl21	NP_001005026.1
Canis lupus familiaris (dog)	RPL21	NP_001238973.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP012138	XP_320389.2
Arabidopsis thaliana (thale cress)	AT1G57660	NP_564724.1
Danio rerio (zebrafish)	rpl21	NP_001002155.1
Saccharomyces cerevisiae (baker's yeast)	RPL21A	NP_009750.1
Eremothecium gossypii	AGOS_AEL254W	NP_984607.1
Schizosaccharomyces pombe (fission yeast)	rpl2101	NP_596032.1
Arabidopsis thaliana (thale cress)	AT1G57860	NP_564726.1
Oryza sativa (rice)	Os03g0141000	NP_001173260.1

Gene Ontology
Bibliography

Related articles in PubMed

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Jefferson M, Donaszi-Ivanov A, Pollen S, Dalmay T, Saalbach G, Powell PP
Journal of virology88(18)10340-53(2014 Sep)

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Castello A, Fischer B, Eichelbaum K, Horos R, Beckmann BM, Strein C, Davey NE, Humphreys DT, Preiss T, Steinmetz LM, Krijgsveld J, Hentze MW
Cell149(6)1393-406(2012 Jun)

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Baltz AG, Munschauer M, Schwanh?usser B, Vasile A, Murakawa Y, Schueler M, Youngs N, Penfold-Brown D, Drew K, Milek M, Wyler E, Bonneau R, Selbach M, Dieterich C, Landthaler M
Molecular cell46(5)674-90(2012 Jun)

Mutation in ribosomal protein L21 underlies hereditary hypotrichosis simplex.
Zhou C, Zang D, Jin Y, Wu H, Liu Z, Du J, Zhang J
Human mutation32(7)710-4(2011 Jul)

Defining the membrane proteome of NK cells.
Ghosh D, Lippert D, Krokhin O, Cortens JP, Wilkins JA
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The cause of hereditary hypotrichosis in this family was identified as a c.95G>A (p.Arg32Gln) mutation in the ribosomal protein L21.
Title: Mutation in ribosomal protein L21 underlies hereditary hypotrichosis simplex.

The following RPL21 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RPL21 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OHu30529	NM_000982.3 Latest version!	Homo sapiens ribosomal protein L21 (RPL21), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RPL21 ( NM_000982.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000973.2 Homo sapiens ribosomal protein L21 (RPL21), mRNA.

Price & Availability↑

CloneID	OHu30529
Clone ID Related Accession (Same CDS sequence)	NM_000982.3
Accession Version	NM_000982.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 483bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-22
Organism	Homo sapiens(human)
Product	60S ribosomal protein L21
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff in collaboration with Francesco Amaldi. The reference sequence was derived from D28406.1, U14967.1 and BC001603.1. This sequence is a reference standard in the RefSeqGene project. On Oct 27, 2005 this sequence version replaced NM_000982.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP203966.1, SRR1163657.538040.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGACGAACA CAAAGGGAAA GAGGAGAGGC ACCCGATATA TGTTCTCTAG GCCTTTTAGA 
AAACATGGAG TTGTTCCTTT GGCCACATAT ATGCGAATCT ATAAGAAAGG TGATATTGTA 
GACATCAAGG GAATGGGTAC TGTTCAAAAA GGAATGCCCC ACAAGTGTTA CCATGGCAAA 
ACTGGAAGAG TCTACAATGT TACCCAGCAT GCTGTTGGCA TTGTTGTAAA CAAACAAGTT 
AAGGGCAAGA TTCTTGCCAA GAGAATTAAT GTGCGTATTG AGCACATTAA GCACTCTAAG 
AGCCGAGATA GCTTCCTGAA ACGTGTGAAG GAAAATGATC AGAAAAAGAA AGAAGCCAAA 
GAGAAAGGTA CCTGGGTTCA ACTAAAGCGC CAGCCTGCTC CACCCAGAGA AGCACACTTT 
GTGAGAACCA ATGGGAAGGA GCCTGAGCTG CTGGAACCTA TTCCCTATGA ATTCATGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000973.2
CDS	44..526
Translation	MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA

Target ORF information:

RefSeq Version	NM_000982.3
Organism	Homo sapiens(human)
Definition	Homo sapiens ribosomal protein L21 (RPL21), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000982.3

ORF Insert Sequence:

ATGACGAACA CAAAGGGAAA GAGGAGAGGC ACCCGATATA TGTTCTCTAG GCCTTTTAGA 
AAACATGGAG TTGTTCCTTT GGCCACATAT ATGCGAATCT ATAAGAAAGG TGATATTGTA 
GACATCAAGG GAATGGGTAC TGTTCAAAAA GGAATGCCCC ACAAGTGTTA CCATGGCAAA 
ACTGGAAGAG TCTACAATGT TACCCAGCAT GCTGTTGGCA TTGTTGTAAA CAAACAAGTT 
AAGGGCAAGA TTCTTGCCAA GAGAATTAAT GTGCGTATTG AGCACATTAA GCACTCTAAG 
AGCCGAGATA GCTTCCTGAA ACGTGTGAAG GAAAATGATC AGAAAAAGAA AGAAGCCAAA 
GAGAAAGGTA CCTGGGTTCA ACTAAAGCGC CAGCCTGCTC CACCCAGAGA AGCACACTTT 
GTGAGAACCA ATGGGAAGGA GCCTGAGCTG CTGGAACCTA TTCCCTATGA ATTCATGGCA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Host factors that interact with the pestivirus N-terminal protease, Npro, are components of the ribonucleoprotein complex.
Journal of virology88(18)10340-53(2014 Sep)
Jefferson M,Donaszi-Ivanov A,Pollen S,Dalmay T,Saalbach G,Powell PP

Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.
Cell149(6)1393-406(2012 Jun)
Castello A,Fischer B,Eichelbaum K,Horos R,Beckmann BM,Strein C,Davey NE,Humphreys DT,Preiss T,Steinmetz LM,Krijgsveld J,Hentze MW

The mRNA-bound proteome and its global occupancy profile on protein-coding transcripts.
Molecular cell46(5)674-90(2012 Jun)
Baltz AG,Munschauer M,Schwanh?usser B,Vasile A,Murakawa Y,Schueler M,Youngs N,Penfold-Brown D,Drew K,Milek M,Wyler E,Bonneau R,Selbach M,Dieterich C,Landthaler M

Mutation in ribosomal protein L21 underlies hereditary hypotrichosis simplex.
Human mutation32(7)710-4(2011 Jul)
Zhou C,Zang D,Jin Y,Wu H,Liu Z,Du J,Zhang J

Defining the membrane proteome of NK cells.
Journal of mass spectrometry : JMS45(1)1-25(2010 Jan)
Ghosh D,Lippert D,Krokhin O,Cortens JP,Wilkins JA

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RPL21 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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