ROBO2 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ROBO2
Entrez Gene ID	6092
Full Name	roundabout guidance receptor 2
Synonyms	SAX3
General protein information	Preferred Names roundabout guidance receptor 2 Names roundabout homolog 2 roundabout, axon guidance receptor, homolog 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3p12.3
Summary	The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014].
Disorder MIM:	602431
Disorder Html:	Vesicoureteral reflux 2, 610878 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002942.4	NP_002933.1	roundabout homolog 2 isoform ROBO2b precursor
XM_011533983.1	XP_011532285.1	roundabout homolog 2 isoform X5
XM_017006996.1	XP_016862485.1	roundabout homolog 2 isoform X15
XM_017006998.1	XP_016862487.1	roundabout homolog 2 isoform X17
XM_017007000.1	XP_016862489.1	roundabout homolog 2 isoform X19
XM_011533985.1	XP_011532287.1	roundabout homolog 2 isoform X20
XM_011533981.2	XP_011532283.1	roundabout homolog 2 isoform X1
XM_017006986.1	XP_016862475.1	roundabout homolog 2 isoform X2
XM_011533982.1	XP_011532284.1	roundabout homolog 2 isoform X3
XM_017006987.1	XP_016862476.1	roundabout homolog 2 isoform X4
XM_017006988.1	XP_016862477.1	roundabout homolog 2 isoform X6
XM_017006990.1	XP_016862479.1	roundabout homolog 2 isoform X8
XM_017006993.1	XP_016862482.1	roundabout homolog 2 isoform X11
XM_017006995.1	XP_016862484.1	roundabout homolog 2 isoform X14
XM_017007002.1	XP_016862491.1	roundabout homolog 2 isoform X22
XM_017007005.1	XP_016862494.1	roundabout homolog 2 isoform X25
XM_017007003.1	XP_016862492.1	roundabout homolog 2 isoform X23
XM_017007004.1	XP_016862493.1	roundabout homolog 2 isoform X24
XM_017007006.1	XP_016862495.1	roundabout homolog 2 isoform X26
XM_017006997.1	XP_016862486.1	roundabout homolog 2 isoform X16
XM_017006999.1	XP_016862488.1	roundabout homolog 2 isoform X18
XM_017006989.1	XP_016862478.1	roundabout homolog 2 isoform X7
XM_017006991.1	XP_016862480.1	roundabout homolog 2 isoform X9
NM_001290039.2	NP_001276968.1	roundabout homolog 2 isoform 3 precursor
NM_002942.5	NP_002933.1	roundabout homolog 2 isoform ROBO2b precursor
NM_001290039.1	NP_001276968.1	roundabout homolog 2 isoform 3 precursor
NM_001290065.1	NP_001276994.1	roundabout homolog 2 isoform 5
NM_001290040.1	NP_001276969.1	roundabout homolog 2 isoform 4 precursor
XM_017006992.1	XP_016862481.1	roundabout homolog 2 isoform X10
XM_011533984.1	XP_011532286.1	roundabout homolog 2 isoform X12
XM_017006994.1	XP_016862483.1	roundabout homolog 2 isoform X13
XM_017007001.1	XP_016862490.1	roundabout homolog 2 isoform X21
NM_001128929.3	NP_001122401.1	roundabout homolog 2 isoform ROBO2a
NM_001290040.2	NP_001276969.1	roundabout homolog 2 isoform 4 precursor
NM_001290065.2	NP_001276994.1	roundabout homolog 2 isoform 5

Pathways from BioSystems

Homologies

Homo sapiens (human)	ROBO2	NP_002933.1
Rattus norvegicus (Norway rat)	Robo2	NP_115289.1
Canis lupus familiaris (dog)	LOC487691	XP_005638813.1
Bos taurus (cattle)	LOC534842	XP_002684698.3
Gallus gallus (chicken)	ROBO2	XP_416674.4
Danio rerio (zebrafish)	robo2	NP_571708.1
	LOC719197	XP_002802664.1
Mus musculus (house mouse)	Robo2	NP_780758.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP010969	XP_309726.4
Drosophila melanogaster (fruit fly)	robo	NP_476899.1
Xenopus tropicalis (tropical clawed frog)	robo2	XP_004912137.1
Pan troglodytes (chimpanzee)	ROBO2	XP_516591.3

Gene Ontology
Bibliography

Related articles in PubMed

Robo Ig4 Is a Dimerization Domain.
Yom-Tov G, Barak R, Matalon O, Barda-Saad M, Guez-Haddad J, Opatowsky Y
Journal of molecular biology429(23)3606-3616(2017 Nov)

ROBO2 gene variants in children with primary nonsyndromic vesicoureteral reflux with or without renal hypoplasia/dysplasia.
Mitsioni AG, Siomou E, Bouba I, Petridi S, Siamopoulou A, Georgiou I
Pediatric research80(1)72-6(2016 07)

Correlations between gastric cancer family history and ROBO2 and RASSF2A gene methylations.
Chang ZW, Dong L, Qin YR, Song M, Guo HY, Zhu QL
Journal of cancer research and therapeutics12(2)597-600(2016 Apr-Jun)

Human placental multipotent mesenchymal stromal cells modulate placenta angiogenesis through Slit2-Robo signaling.
Chen CY, Tsai CH, Chen CY, Wu YH, Chen CP
Cell adhesion & migration10(1-2)66-76(2016 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

determined the crystal structure of the tandem Ig4-5 domains (D4-5) of human Robo2 and found that a hydrophobic surface on D4 mediates close homotypic contacts with a reciprocal D4
Title: Robo Ig4 Is a Dimerization Domain.

ROBO2 variants were not found to be associated with nonsyndromic vesicoureteral reflux with or without renal hypoplasia/dysplasia, providing further evidence for genetic heterogeneity
Title: ROBO2 gene variants in children with primary nonsyndromic vesicoureteral reflux with or without renal hypoplasia/dysplasia.

ROBO2 and RASSF2A gene methylations may be related to gastric tumorigenesis, and ROBO2 gene methylation is associated with sporadic gastric cancer.
Title: Correlations between gastric cancer family history and ROBO2 and RASSF2A gene methylations.

human placental multipotent mesenchymal stromal cell express Slit2 and both Robo1 and Robo4 are present in human umbilical vein endothelial cells.
Title: Human placental multipotent mesenchymal stromal cells modulate placenta angiogenesis through Slit2-Robo signaling.

ROBO2 somatic mutation is associated with myelodysplastic syndrome progression. Overexpression of ROBO2 produces anti-proliferative and pro-apoptotic effects in leukemia cells. This effect was lost in ROBO mutants and ROBO-SLIT2 signaling is impaired.
Title: Whole-exome and targeted sequencing identify ROBO1 and ROBO2 mutations as progression-related drivers in myelodysplastic syndromes.

The following ROBO2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ROBO2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22766	NM_001128929.3 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu12688	NM_001290065.2 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu94465	XM_017006991.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94466	XM_017006992.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94467	XM_017006993.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94468	XM_017006994.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94469	XM_017006995.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94470	XM_017006996.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94471	XM_017006997.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94472	XM_017006998.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94473	XM_017006999.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94474	XM_017007000.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94475	XM_017007001.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94476	XM_017007002.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94477	XM_017007003.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94478	XM_017007004.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94479	XM_017007005.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94480	XM_017007006.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OHu94460	XM_017006986.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu94461	XM_017006987.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu94462	XM_017006988.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu94463	XM_017006989.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu94464	XM_017006990.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$811.30 $1159.00
OHu11403	NM_002942.5 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu12785	NM_001290040.2 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu12905	NM_001290039.2 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu60280	XM_011533981.2 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu60281	XM_011533982.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu60282	XM_011533983.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$811.30 $1159.00
OHu60283	XM_011533984.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu60284	XM_011533985.1 Latest version!	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
View Old Accession Versions >>
OHu11403	NM_002942.4	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$923.30 $1319.00
OHu12905	NM_001290039.1	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu12785	NM_001290040.1	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$727.30 $1039.00
OHu12688	NM_001290065.1	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ROBO2 ( NM_001128929.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001122401.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22766
Clone ID Related Accession (Same CDS sequence)	NM_001128929.3
Accession Version	NM_001128929.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4185bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-22
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform ROBO2a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC126467.6, DQ533873.1 and AC133040.3. This sequence is a reference standard in the RefSeqGene project. On Feb 22, 2014 this sequence version replaced NM_001128929.2. Transcript Variant: This variant (1) represents the use of an alternate promoter and has multiple differences compared to variant 4. These differences result in a distinct 5' UTR and the use of an alternate start codon compared to variant 4. The encoded protein (isoform ROBO2a; PMID 16829019) has a distinct N-terminus and is shorter than isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ533873.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCCCCCA CAGTTTGTGG TTCGGCCAAG AGATCAGATT 
GTTGCTCAAG GTCGAACAGT GACATTTCCC TGTGAAACTA AAGGAAACCC ACAGCCAGCT 
GTTTTTTGGC AGAAAGAAGG CAGCCAGAAC CTACTTTTCC CAAACCAACC CCAGCAGCCC 
AACAGTAGAT GCTCAGTGTC ACCAACTGGA GACCTCACAA TCACCAACAT TCAACGTTCC 
GACGCGGGTT ACTACATCTG CCAGGCTTTA ACTGTGGCAG GAAGCATTTT AGCAAAAGCT 
CAACTGGAGG TTACTGATGT TTTGACAGAT AGACCTCCAC CTATAATTCT ACAAGGCCCA 
GCCAACCAAA CGCTGGCAGT GGATGGTACA GCGTTACTGA AATGTAAAGC CACTGGTGAT 
CCTCTTCCTG TAATTAGCTG GTTAAAGGAG GGATTTACTT TTCCGGGTAG AGATCCAAGA 
GCAACAATTC AAGAGCAAGG CACACTGCAG ATTAAGAATT TACGGATTTC TGATACTGGC 
ACTTATACTT GTGTGGCTAC AAGTTCAAGT GGAGAGACTT CCTGGAGTGC AGTGCTGGAT 
GTGACAGAGT CTGGAGCAAC AATCAGTAAA AACTATGATT TAAGTGACCT GCCAGGGCCA 
CCATCCAAAC CGCAGGTCAC TGATGTTACT AAGAACAGTG TCACCTTGTC CTGGCAGCCA 
GGTACCCCTG GAACCCTTCC AGCAAGTGCA TATATCATTG AGGCTTTCAG CCAATCAGTG 
AGCAACAGCT GGCAGACCGT GGCAAACCAT GTAAAGACCA CCCTCTATAC TGTAAGAGGA 
CTGCGGCCCA ATACAATCTA CTTATTCATG GTCAGAGCGA TCAACCCCCA AGGTCTCAGT 
GACCCAAGTC CCATGTCAGA TCCTGTGCGC ACACAAGATA TCAGCCCACC AGCACAAGGA 
GTGGACCACA GGCAAGTGCA GAAAGAGCTA GGAGATGTCC TTGTCCGTCT TCATAATCCA 
GTTGTGCTGA CTCCCACCAC GGTTCAGGTC ACATGGACGG TTGATCGCCA ACCCCAGTTT 
ATCCAAGGCT ACCGAGTGAT GTATCGTCAG ACTTCAGGTC TGCAGGCGAC ATCTTCGTGG 
CAGAATTTAG ATGCCAAAGT CCCGACTGAA CGAAGTGCTG TCTTAGTCAA CCTGAAAAAG 
GGGGTGACTT ATGAAATTAA AGTACGGCCA TATTTTAATG AGTTCCAAGG AATGGATAGT 
GAATCTAAAA CGGTTCGTAC TACTGAAGAA GCCCCAAGTG CCCCACCACA GTCTGTCACT 
GTACTGACAG TTGGAAGCTA CAATAGCACA AGTATTAGTG TTTCCTGGGA TCCTCCTCCT 
CCAGATCACC AGAATGGAAT TATCCAAGAA TACAAGATCT GGTGTCTAGG AAATGAAACG 
CGATTCCATA TCAACAAAAC TGTGGATGCA GCCATTCGGT CCGTAATAAT TGGTGGATTA 
TTCCCAGGTA TTCAATACCG GGTAGAGGTT GCAGCTAGTA CCAGTGCAGG GGTTGGAGTA 
AAGAGTGAGC CACAGCCAAT AATAATCGGG AGACGCAATG AAGTTGTCAT TACTGAAAAC 
AATAACAGCA TAACTGAGCA AATCACTGAT GTGGTGAAGC AACCAGCCTT TATAGCTGGT 
ATTGGTGGTG CCTGCTGGGT AATTCTGATG GGTTTTAGCA TATGGTTGTA TTGGCGAAGA 
AAGAAGAGGA AGGGACTCAG TAATTATGCT GTTACGTTTC AAAGAGGAGA TGGAGGACTA 
ATGAGCAATG GAAGCCGTCC AGGTCTTCTC AATGCTGGTG ATCCCAGCTA TCCATGGCTT 
GCTGATTCTT GGCCAGCCAC GAGCTTGCCA GTAAATAATA GCAACAGTGG CCCAAATGAG 
ATTGGAAATT TTGGCCGTGG AGATGTGCTG CCACCAGTTC CAGGCCAAGG GGATAAAACA 
GCAACGATGC TCTCAGATGG AGCCATTTAT AGTAGCATTG ACTTCACTAC CAAAACCAGT 
TACAACAGTT CCAGCCAAAT AACACAGGCT ACCCCATATG CCACGACACA GATCTTGCAT 
TCCAACAGCA TACATGAATT GGCTGTCGAT CTGCCTGATC CACAATGGAA AAGCTCAATT 
CAGCAAAAAA CAGATCTGAT GGGATTTGGT TATTCTCTAC CTGATCAGAA CAAAGGTAAC 
AATGGTGGGA AAGGTGGAAA AAAGAAGAAA AATAAAAACT CTTCTAAACC ACAGAAAAAC 
AATGGATCCA CTTGGGCCAA TGTCCCTCTA CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC 
ACGGAGCTGG AACACTATGC AGTGGAACAA CAAGAAAATG GCTATGACAG TGATAGCTGG 
TGCCCACCAT TGCCAGTACA AACTTACTTA CACCAAGGTC TGGAAGATGA ACTGGAAGAA 
GATGATGATA GGGTCCCAAC ACCTCCTGTT CGAGGCGTGG CTTCTTCTCC TGCTATCTCC 
TTTGGACAGC AGTCCACTGC AACTCTTACT CCATCCCCAC GGGAAGAGAT GCAACCCATG 
CTGCAGGCTC ACCTGGATGA GTTGACAAGA GCCTATCAGT TTGATATAGC AAAACAAACA 
TGGCACATTC AAAGCAATAA TCAACCTCCA CAGCCTCCAG TTCCACCGTT AGGTTATGTG 
TCTGGAGCCT TGATTTCTGA TTTGGAAACG GATGTTGCAG ATGATGATGC CGACGACGAA 
GAGGAAGCTT TAGAAATCCC CAGGCCCCTG AGAGCACTGG ACCAGACTCC TGGATCCAGC 
ATGGACAATC TAGACAGCTC TGTGACAGGA AAAGCCTTTA CCTCCTCTCA AAGACCTCGA 
CCTACCAGCC CATTTTCTAC TGACAGTAAC ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG 
CCTCGGCCCA CTAAAAAACA CAAGGGAGGG CGGATGGACC AACAACCAGC ATTGCCTCAT 
CGAAGGGAAG GAATGACAGA TGAGGAGGCC TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA 
TCTCCAGGTG GCCACAGCTC ATCAGGAACA GCTTCTTCTA AGGGATCCAC TGGACCTAGG 
AAAACCGAGG TGTTGAGAGC AGGCCACCAG CGCAATGCCA GCGACCTTCT TGACATAGGA 
TATATGGGCT CCAACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001122401.1
CDS	300..4484
Translation	MARRHERVTRRMWTWAPGLLMMTVVFWGHQGNGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	NM_001128929.3
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128929.3

ORF Insert Sequence:

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCCCCCA CAGTTTGTGG TTCGGCCAAG AGATCAGATT 
GTTGCTCAAG GTCGAACAGT GACATTTCCC TGTGAAACTA AAGGAAACCC ACAGCCAGCT 
GTTTTTTGGC AGAAAGAAGG CAGCCAGAAC CTACTTTTCC CAAACCAACC CCAGCAGCCC 
AACAGTAGAT GCTCAGTGTC ACCAACTGGA GACCTCACAA TCACCAACAT TCAACGTTCC 
GACGCGGGTT ACTACATCTG CCAGGCTTTA ACTGTGGCAG GAAGCATTTT AGCAAAAGCT 
CAACTGGAGG TTACTGATGT TTTGACAGAT AGACCTCCAC CTATAATTCT ACAAGGCCCA 
GCCAACCAAA CGCTGGCAGT GGATGGTACA GCGTTACTGA AATGTAAAGC CACTGGTGAT 
CCTCTTCCTG TAATTAGCTG GTTAAAGGAG GGATTTACTT TTCCGGGTAG AGATCCAAGA 
GCAACAATTC AAGAGCAAGG CACACTGCAG ATTAAGAATT TACGGATTTC TGATACTGGC 
ACTTATACTT GTGTGGCTAC AAGTTCAAGT GGAGAGACTT CCTGGAGTGC AGTGCTGGAT 
GTGACAGAGT CTGGAGCAAC AATCAGTAAA AACTATGATT TAAGTGACCT GCCAGGGCCA 
CCATCCAAAC CGCAGGTCAC TGATGTTACT AAGAACAGTG TCACCTTGTC CTGGCAGCCA 
GGTACCCCTG GAACCCTTCC AGCAAGTGCA TATATCATTG AGGCTTTCAG CCAATCAGTG 
AGCAACAGCT GGCAGACCGT GGCAAACCAT GTAAAGACCA CCCTCTATAC TGTAAGAGGA 
CTGCGGCCCA ATACAATCTA CTTATTCATG GTCAGAGCGA TCAACCCCCA AGGTCTCAGT 
GACCCAAGTC CCATGTCAGA TCCTGTGCGC ACACAAGATA TCAGCCCACC AGCACAAGGA 
GTGGACCACA GGCAAGTGCA GAAAGAGCTA GGAGATGTCC TTGTCCGTCT TCATAATCCA 
GTTGTGCTGA CTCCCACCAC GGTTCAGGTC ACATGGACGG TTGATCGCCA ACCCCAGTTT 
ATCCAAGGCT ACCGAGTGAT GTATCGTCAG ACTTCAGGTC TGCAGGCGAC ATCTTCGTGG 
CAGAATTTAG ATGCCAAAGT CCCGACTGAA CGAAGTGCTG TCTTAGTCAA CCTGAAAAAG 
GGGGTGACTT ATGAAATTAA AGTACGGCCA TATTTTAATG AGTTCCAAGG AATGGATAGT 
GAATCTAAAA CGGTTCGTAC TACTGAAGAA GCCCCAAGTG CCCCACCACA GTCTGTCACT 
GTACTGACAG TTGGAAGCTA CAATAGCACA AGTATTAGTG TTTCCTGGGA TCCTCCTCCT 
CCAGATCACC AGAATGGAAT TATCCAAGAA TACAAGATCT GGTGTCTAGG AAATGAAACG 
CGATTCCATA TCAACAAAAC TGTGGATGCA GCCATTCGGT CCGTAATAAT TGGTGGATTA 
TTCCCAGGTA TTCAATACCG GGTAGAGGTT GCAGCTAGTA CCAGTGCAGG GGTTGGAGTA 
AAGAGTGAGC CACAGCCAAT AATAATCGGG AGACGCAATG AAGTTGTCAT TACTGAAAAC 
AATAACAGCA TAACTGAGCA AATCACTGAT GTGGTGAAGC AACCAGCCTT TATAGCTGGT 
ATTGGTGGTG CCTGCTGGGT AATTCTGATG GGTTTTAGCA TATGGTTGTA TTGGCGAAGA 
AAGAAGAGGA AGGGACTCAG TAATTATGCT GTTACGTTTC AAAGAGGAGA TGGAGGACTA 
ATGAGCAATG GAAGCCGTCC AGGTCTTCTC AATGCTGGTG ATCCCAGCTA TCCATGGCTT 
GCTGATTCTT GGCCAGCCAC GAGCTTGCCA GTAAATAATA GCAACAGTGG CCCAAATGAG 
ATTGGAAATT TTGGCCGTGG AGATGTGCTG CCACCAGTTC CAGGCCAAGG GGATAAAACA 
GCAACGATGC TCTCAGATGG AGCCATTTAT AGTAGCATTG ACTTCACTAC CAAAACCAGT 
TACAACAGTT CCAGCCAAAT AACACAGGCT ACCCCATATG CCACGACACA GATCTTGCAT 
TCCAACAGCA TACATGAATT GGCTGTCGAT CTGCCTGATC CACAATGGAA AAGCTCAATT 
CAGCAAAAAA CAGATCTGAT GGGATTTGGT TATTCTCTAC CTGATCAGAA CAAAGGTAAC 
AATGGTGGGA AAGGTGGAAA AAAGAAGAAA AATAAAAACT CTTCTAAACC ACAGAAAAAC 
AATGGATCCA CTTGGGCCAA TGTCCCTCTA CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC 
ACGGAGCTGG AACACTATGC AGTGGAACAA CAAGAAAATG GCTATGACAG TGATAGCTGG 
TGCCCACCAT TGCCAGTACA AACTTACTTA CACCAAGGTC TGGAAGATGA ACTGGAAGAA 
GATGATGATA GGGTCCCAAC ACCTCCTGTT CGAGGCGTGG CTTCTTCTCC TGCTATCTCC 
TTTGGACAGC AGTCCACTGC AACTCTTACT CCATCCCCAC GGGAAGAGAT GCAACCCATG 
CTGCAGGCTC ACCTGGATGA GTTGACAAGA GCCTATCAGT TTGATATAGC AAAACAAACA 
TGGCACATTC AAAGCAATAA TCAACCTCCA CAGCCTCCAG TTCCACCGTT AGGTTATGTG 
TCTGGAGCCT TGATTTCTGA TTTGGAAACG GATGTTGCAG ATGATGATGC CGACGACGAA 
GAGGAAGCTT TAGAAATCCC CAGGCCCCTG AGAGCACTGG ACCAGACTCC TGGATCCAGC 
ATGGACAATC TAGACAGCTC TGTGACAGGA AAAGCCTTTA CCTCCTCTCA AAGACCTCGA 
CCTACCAGCC CATTTTCTAC TGACAGTAAC ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG 
CCTCGGCCCA CTAAAAAACA CAAGGGAGGG CGGATGGACC AACAACCAGC ATTGCCTCAT 
CGAAGGGAAG GAATGACAGA TGAGGAGGCC TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA 
TCTCCAGGTG GCCACAGCTC ATCAGGAACA GCTTCTTCTA AGGGATCCAC TGGACCTAGG 
AAAACCGAGG TGTTGAGAGC AGGCCACCAG CGCAATGCCA GCGACCTTCT TGACATAGGA 
TATATGGGCT CCAACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( NM_001290065.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276994.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu12688
Clone ID Related Accession (Same CDS sequence)	NM_001290065.1 , NM_001290065.2
Accession Version	NM_001290065.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ533874.1, BC144302.1 and AC133040.3. Transcript Variant: This variant (5) has multiple differences compared to variant 4. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon compared to variant 4; the 5'-most initiation codon, as used in variant 4, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG resulting in an isoform (5) with a shorter N-terminus compared to isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144302.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465404 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG 
AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT 
CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA 
CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC 
TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT 
GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT 
ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA 
AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT 
CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA 
ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC 
CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA 
GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT 
AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA 
GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG 
GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA 
ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC 
CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG 
TTTACAGGTG AATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276994.1
CDS	2562..4919
Translation	MVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	NM_001290065.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290065.1

ORF Insert Sequence:

ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG 
AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT 
CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA 
CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC 
TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT 
GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT 
ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA 
AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT 
CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA 
ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC 
CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA 
GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT 
AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA 
GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG 
GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA 
ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC 
CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG 
TTTACAGGTG AATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( NM_001290065.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276994.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu12688
Clone ID Related Accession (Same CDS sequence)	NM_001290065.1 , NM_001290065.2
Accession Version	NM_001290065.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2358bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform 5
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117515.8, DQ533874.1, BC144302.1 and AC133040.3. On Jun 2, 2019 this sequence version replaced NM_001290065.1. Transcript Variant: This variant (5) has multiple differences compared to variant 4. These differences result in a distinct 5' UTR and cause translation initiation at a downstream start codon compared to variant 4; the 5'-most initiation codon, as used in variant 4, is associated with a truncated ORF that would render the transcript a candidate for nonsense-mediated decay (NMD). Leaky scanning may allow translation initiation at the downstream AUG resulting in an isoform (5) with a shorter N-terminus compared to isoform 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144302.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03465404 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG 
AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT 
CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA 
CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC 
TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT 
GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT 
ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA 
AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT 
CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA 
ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC 
CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA 
GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT 
AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA 
GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG 
GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA 
ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC 
CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG 
TTTACAGGTG AATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276994.1
CDS	2606..4963
Translation	MVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	NM_001290065.2
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290065.2

ORF Insert Sequence:

ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG 
AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT 
CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA 
CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC 
TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT 
GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT 
ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA 
AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT 
CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA 
ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC 
CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA 
GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT 
AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA 
GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG 
GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA 
ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC 
CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG 
TTTACAGGTG AATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006991.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862480.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X9, mRNA.

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CloneID	OHu94465
Clone ID Related Accession (Same CDS sequence)	XM_017006991.1
Accession Version	XM_017006991.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4491bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG 
AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT 
CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA 
CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC 
TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT 
GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT 
ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA 
AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT 
CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA 
ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC 
CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA 
GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT 
AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA 
GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGATCTT 
CCACCACCAC CAGATCCCCC GCCAGGTCAG GGTTTAAGGC AGCAAATAGG CCCGAGCCAG 
CAGGCTGGTA ACGTGGAAAA CTCAGCAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAT 
GCATCCAGCT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAGAAC CTCCCTAGAG 
TGGCAGCGAC AAACCCAGGA ATGGATAAGC TCCACAGAAC GACAAGAAGA TATACGGAAA 
GCCCCACACA AACAAGGTGT CGGATCAGAG GAGGCCTTGG TGCCCTATAG CAAGCCCAGT 
TTCCCATCTC CAGGTGGCCA CAGCTCATCA GGAACAGCTT CTTCTAAGGG ATCCACTGGA 
CCTAGGAAAA CCGAGGTGTT GAGAGCAGGC CACCAGCGCA ATGCCAGCGA CCTTCTTGAC 
ATAGGATATA TGGGCTCCAA CAGTCAAGGA CAGTTTACAG GTGAATTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862480.1
CDS	400..4890
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDDLPPPPDPPPGQGLRQQIGPSQQAGNVENSAERKGSSLERQHASSLEDTKSSLDCPARTSLEWQRQTQEWISSTERQEDIRKAPHKQGVGSEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017006991.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006991.1

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG 
AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT 
CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA 
CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC 
TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT 
GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT 
ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA 
AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT 
CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA 
ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC 
CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA 
GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT 
AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA 
GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGATCTT 
CCACCACCAC CAGATCCCCC GCCAGGTCAG GGTTTAAGGC AGCAAATAGG CCCGAGCCAG 
CAGGCTGGTA ACGTGGAAAA CTCAGCAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAT 
GCATCCAGCT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAGAAC CTCCCTAGAG 
TGGCAGCGAC AAACCCAGGA ATGGATAAGC TCCACAGAAC GACAAGAAGA TATACGGAAA 
GCCCCACACA AACAAGGTGT CGGATCAGAG GAGGCCTTGG TGCCCTATAG CAAGCCCAGT 
TTCCCATCTC CAGGTGGCCA CAGCTCATCA GGAACAGCTT CTTCTAAGGG ATCCACTGGA 
CCTAGGAAAA CCGAGGTGTT GAGAGCAGGC CACCAGCGCA ATGCCAGCGA CCTTCTTGAC 
ATAGGATATA TGGGCTCCAA CAGTCAAGGA CAGTTTACAG GTGAATTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006992.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862481.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OHu94466
Clone ID Related Accession (Same CDS sequence)	XM_017006992.1
Accession Version	XM_017006992.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4470bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA 
CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG 
CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC 
AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT 
GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT 
CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG 
TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT 
GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT 
CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT 
CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT 
CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA 
GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGATCTTC CACCACCACC AGATCCCCCG 
CCAGGTCAGG GTTTAAGGCA GCAAATAGGC CCGAGCCAGC AGGCTGGTAA CGTGGAAAAC 
TCAGCAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACATG CATCCAGCTT AGAAGACACA 
AAGAGCTCAT TGGATTGTCC AGCTAGAACC TCCCTAGAGT GGCAGCGACA AACCCAGGAA 
TGGATAAGCT CCACAGAACG ACAAGAAGAT ATACGGAAAG CCCCACACAA ACAAGGTGTC 
GGATCAGAGG AGGCCTTGGT GCCCTATAGC AAGCCCAGTT TCCCATCTCC AGGTGGCCAC 
AGCTCATCAG GAACAGCTTC TTCTAAGGGA TCCACTGGAC CTAGGAAAAC CGAGGTGTTG 
AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC CTTCTTGACA TAGGATATAT GGGCTCCAAC 
AGTCAAGGAC AGTTTACAGG TGAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862481.1
CDS	300..4769
Translation	MARRHERVTRRMWTWAPGLLMMTVVFWGHQGNGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDDLPPPPDPPPGQGLRQQIGPSQQAGNVENSAERKGSSLERQHASSLEDTKSSLDCPARTSLEWQRQTQEWISSTERQEDIRKAPHKQGVGSEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017006992.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006992.1

ORF Insert Sequence:

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA 
CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG 
CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC 
AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT 
GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT 
CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG 
TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT 
GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT 
CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT 
CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT 
CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA 
GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGATCTTC CACCACCACC AGATCCCCCG 
CCAGGTCAGG GTTTAAGGCA GCAAATAGGC CCGAGCCAGC AGGCTGGTAA CGTGGAAAAC 
TCAGCAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACATG CATCCAGCTT AGAAGACACA 
AAGAGCTCAT TGGATTGTCC AGCTAGAACC TCCCTAGAGT GGCAGCGACA AACCCAGGAA 
TGGATAAGCT CCACAGAACG ACAAGAAGAT ATACGGAAAG CCCCACACAA ACAAGGTGTC 
GGATCAGAGG AGGCCTTGGT GCCCTATAGC AAGCCCAGTT TCCCATCTCC AGGTGGCCAC 
AGCTCATCAG GAACAGCTTC TTCTAAGGGA TCCACTGGAC CTAGGAAAAC CGAGGTGTTG 
AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC CTTCTTGACA TAGGATATAT GGGCTCCAAC 
AGTCAAGGAC AGTTTACAGG TGAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006993.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862482.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OHu94467
Clone ID Related Accession (Same CDS sequence)	XM_017006993.1
Accession Version	XM_017006993.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4410bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA 
CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG 
CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC 
AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT 
GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT 
CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG 
TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT 
GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT 
CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT 
CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT 
CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA 
GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGATCTTC CACCACCACC AGATCCCCCG 
CCAGGTCAGG GTTTAAGGCA GCAAATAGGC CCGAGCCAGC AGGCTGGTAA CGTGGAAAAC 
TCAGCAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACATG CATCCAGCTT AGAAGACACA 
AAGAGCTCAT TGGATTGTCC AGCTAGAACC TCCCTAGAGT GGCAGCGACA AACCCAGGAA 
TGGATAAGCT CCACAGAACG ACAAGAAGAT ATACGGAAAG CCCCACACAA ACAAGGTGTC 
GGATCAGAGG AGGCCTTGGT GCCCTATAGC AAGCCCAGTT TCCCATCTCC AGGTGGCCAC 
AGCTCATCAG GAACAGCTTC TTCTAAGGGA TCCACTGGAC CTAGGAAAAC CGAGGTGTTG 
AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC CTTCTTGACA TAGGATATAT GGGCTCCAAC 
AGTCAAGGAC AGTTTACAGG TGAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862482.1
CDS	692..5101
Translation	MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDDLPPPPDPPPGQGLRQQIGPSQQAGNVENSAERKGSSLERQHASSLEDTKSSLDCPARTSLEWQRQTQEWISSTERQEDIRKAPHKQGVGSEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017006993.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006993.1

ORF Insert Sequence:

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA 
CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG 
CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC 
AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT 
GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT 
CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG 
TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT 
GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT 
CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT 
CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT 
CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA 
GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGATCTTC CACCACCACC AGATCCCCCG 
CCAGGTCAGG GTTTAAGGCA GCAAATAGGC CCGAGCCAGC AGGCTGGTAA CGTGGAAAAC 
TCAGCAGAGA GAAAAGGAAG CTCTCTAGAG AGACAACATG CATCCAGCTT AGAAGACACA 
AAGAGCTCAT TGGATTGTCC AGCTAGAACC TCCCTAGAGT GGCAGCGACA AACCCAGGAA 
TGGATAAGCT CCACAGAACG ACAAGAAGAT ATACGGAAAG CCCCACACAA ACAAGGTGTC 
GGATCAGAGG AGGCCTTGGT GCCCTATAGC AAGCCCAGTT TCCCATCTCC AGGTGGCCAC 
AGCTCATCAG GAACAGCTTC TTCTAAGGGA TCCACTGGAC CTAGGAAAAC CGAGGTGTTG 
AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC CTTCTTGACA TAGGATATAT GGGCTCCAAC 
AGTCAAGGAC AGTTTACAGG TGAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006994.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862483.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X13, mRNA.

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CloneID	OHu94468
Clone ID Related Accession (Same CDS sequence)	XM_017006994.1
Accession Version	XM_017006994.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4338bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT 
GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA 
GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA 
ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA 
CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA 
ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG 
TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT 
CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT 
TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC 
AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT 
GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT 
GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC 
AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT 
GAGGAGGCCT TGGTGCCCTA TAGCAAGCCC AGTTTCCCAT CTCCAGGTGG CCACAGCTCA 
TCAGGAACAG CTTCTTCTAA GGGATCCACT GGACCTAGGA AAACCGAGGT GTTGAGAGCA 
GGCCACCAGC GCAATGCCAG CGACCTTCTT GACATAGGAT ATATGGGCTC CAACAGTCAA 
GGACAGTTTA CAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862483.1
CDS	400..4737
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_017006994.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006994.1

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT 
GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA 
GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA 
ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA 
CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA 
ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG 
TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT 
CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT 
TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC 
AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT 
GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT 
GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC 
AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT 
GAGGAGGCCT TGGTGCCCTA TAGCAAGCCC AGTTTCCCAT CTCCAGGTGG CCACAGCTCA 
TCAGGAACAG CTTCTTCTAA GGGATCCACT GGACCTAGGA AAACCGAGGT GTTGAGAGCA 
GGCCACCAGC GCAATGCCAG CGACCTTCTT GACATAGGAT ATATGGGCTC CAACAGTCAA 
GGACAGTTTA CAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006995.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862484.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OHu94469
Clone ID Related Accession (Same CDS sequence)	XM_017006995.1
Accession Version	XM_017006995.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4329bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGCC TTACTTTACA TTCCTGACTA CCGATTGGCT 
GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT 
CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA 
AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA 
CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA 
CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA 
CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT 
CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA 
CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG 
GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG 
AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGA 
AAAGCCTTTA CCTCCTCTCA AAGACCTCGA CCTACCAGCC CATTTTCTAC TGACAGTAAC 
ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG 
CGGATGGACC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGAGGAGGCC 
TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA TCTCCAGGTG GCCACAGCTC ATCAGGAACA 
GCTTCTTCTA AGGGATCCAC TGGACCTAGG AAAACCGAGG TGTTGAGAGC AGGCCACCAG 
CGCAATGCCA GCGACCTTCT TGACATAGGA TATATGGGCT CCAACAGTCA AGGACAGTTT 
ACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862484.1
CDS	400..4728
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_017006995.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006995.1

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGCC TTACTTTACA TTCCTGACTA CCGATTGGCT 
GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT 
CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA 
AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA 
CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA 
CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA 
CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT 
CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA 
CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG 
GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG 
AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGA 
AAAGCCTTTA CCTCCTCTCA AAGACCTCGA CCTACCAGCC CATTTTCTAC TGACAGTAAC 
ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG 
CGGATGGACC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGAGGAGGCC 
TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA TCTCCAGGTG GCCACAGCTC ATCAGGAACA 
GCTTCTTCTA AGGGATCCAC TGGACCTAGG AAAACCGAGG TGTTGAGAGC AGGCCACCAG 
CGCAATGCCA GCGACCTTCT TGACATAGGA TATATGGGCT CCAACAGTCA AGGACAGTTT 
ACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006996.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862485.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OHu94470
Clone ID Related Accession (Same CDS sequence)	XM_017006996.1
Accession Version	XM_017006996.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT 
AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA 
GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT 
TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA 
GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC 
TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG 
GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT 
TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG 
GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT 
GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT 
CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT 
GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC 
CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC 
TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC 
CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA 
CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG AGGAGGCCTT GGTGCCCTAT 
AGCAAGCCCA GTTTCCCATC TCCAGGTGGC CACAGCTCAT CAGGAACAGC TTCTTCTAAG 
GGATCCACTG GACCTAGGAA AACCGAGGTG TTGAGAGCAG GCCACCAGCG CAATGCCAGC 
GACCTTCTTG ACATAGGATA TATGGGCTCC AACAGTCAAG GACAGTTTAC AGGTGAATTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862485.1
CDS	300..4622
Translation	MARRHERVTRRMWTWAPGLLMMTVVFWGHQGNGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017006996.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006996.1

ORF Insert Sequence:

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT 
AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA 
GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT 
TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA 
GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC 
TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG 
GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT 
TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG 
GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT 
GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT 
CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT 
GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC 
CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC 
TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC 
CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA 
CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG AGGAGGCCTT GGTGCCCTAT 
AGCAAGCCCA GTTTCCCATC TCCAGGTGGC CACAGCTCAT CAGGAACAGC TTCTTCTAAG 
GGATCCACTG GACCTAGGAA AACCGAGGTG TTGAGAGCAG GCCACCAGCG CAATGCCAGC 
GACCTTCTTG ACATAGGATA TATGGGCTCC AACAGTCAAG GACAGTTTAC AGGTGAATTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006997.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862486.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X16, mRNA.

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CloneID	OHu94471
Clone ID Related Accession (Same CDS sequence)	XM_017006997.1
Accession Version	XM_017006997.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4317bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT 
AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA 
GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT 
TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA 
GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC 
TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG 
GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT 
TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG 
GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT 
GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT 
CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT 
GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC 
CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC 
TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC 
CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA 
CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG AGGAGGCCTT GGTGCCCTAT 
AGCAAGCCCA GTTTCCCATC TCCAGGTGGC CACAGCTCAT CAGGAACAGC TTCTTCTAAG 
GGATCCACTG GACCTAGGAA AACCGAGGTG TTGAGAGCAG GCCACCAGCG CAATGCCAGC 
GACCTTCTTG ACATAGGATA TATGGGCTCC AACAGTCAAG GACAGTTTAC AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862486.1
CDS	300..4616
Translation	MARRHERVTRRMWTWAPGLLMMTVVFWGHQGNGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_017006997.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006997.1

ORF Insert Sequence:

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT 
AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA 
GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT 
TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA 
GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC 
TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG 
GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT 
TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG 
GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT 
GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT 
CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT 
GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC 
CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC 
TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC 
CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA 
CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG AGGAGGCCTT GGTGCCCTAT 
AGCAAGCCCA GTTTCCCATC TCCAGGTGGC CACAGCTCAT CAGGAACAGC TTCTTCTAAG 
GGATCCACTG GACCTAGGAA AACCGAGGTG TTGAGAGCAG GCCACCAGCG CAATGCCAGC 
GACCTTCTTG ACATAGGATA TATGGGCTCC AACAGTCAAG GACAGTTTAC AGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006998.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862487.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X17, mRNA.

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CloneID	OHu94472
Clone ID Related Accession (Same CDS sequence)	XM_017006998.1
Accession Version	XM_017006998.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4275bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGCC TTACTTTACA TTCCTGACTA CCGATTGGCT 
GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT 
CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA 
AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA 
CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA 
CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA 
CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT 
CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA 
CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG 
GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG 
AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGA 
AAAGCCTTTA CCTCCTCTCA AAGACCTCGA CCTACCAGCC CATTTTCTAC TGACAGTAAC 
ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG 
CGGATGGACC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGAGGAGGCC 
TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA TCTCCAGGTG GCCACAGCTC ATCAGGAACA 
GCTTCTTCTA AGGGATCCAC TGGACCTAGG AAAACCGAGG TGTTGAGAGC AGGCCACCAG 
CGCAATGCCA GCGACCTTCT TGACATAGGA TATATGGGCT CCAACAGTCA AGGACAGTTT 
ACAGGTGAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862487.1
CDS	692..4966
Translation	MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017006998.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006998.1

ORF Insert Sequence:

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGCC TTACTTTACA TTCCTGACTA CCGATTGGCT 
GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT 
CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA 
AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA 
CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA 
CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA 
CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT 
CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA 
CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG 
GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG 
AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGA 
AAAGCCTTTA CCTCCTCTCA AAGACCTCGA CCTACCAGCC CATTTTCTAC TGACAGTAAC 
ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG 
CGGATGGACC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGAGGAGGCC 
TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA TCTCCAGGTG GCCACAGCTC ATCAGGAACA 
GCTTCTTCTA AGGGATCCAC TGGACCTAGG AAAACCGAGG TGTTGAGAGC AGGCCACCAG 
CGCAATGCCA GCGACCTTCT TGACATAGGA TATATGGGCT CCAACAGTCA AGGACAGTTT 
ACAGGTGAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006999.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862488.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X18, mRNA.

Price & Availability↑

CloneID	OHu94473
Clone ID Related Accession (Same CDS sequence)	XM_017006999.1
Accession Version	XM_017006999.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGCC TTACTTTACA TTCCTGACTA CCGATTGGCT 
GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT 
CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA 
AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA 
CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA 
CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA 
CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT 
CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA 
CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG 
GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG 
AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGA 
AAAGCCTTTA CCTCCTCTCA AAGACCTCGA CCTACCAGCC CATTTTCTAC TGACAGTAAC 
ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG 
CGGATGGACC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGAGGAGGCC 
TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA TCTCCAGGTG GCCACAGCTC ATCAGGAACA 
GCTTCTTCTA AGGGATCCAC TGGACCTAGG AAAACCGAGG TGTTGAGAGC AGGCCACCAG 
CGCAATGCCA GCGACCTTCT TGACATAGGA TATATGGGCT CCAACAGTCA AGGACAGTTT 
ACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862488.1
CDS	692..4960
Translation	MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_017006999.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006999.1

ORF Insert Sequence:

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGCC TTACTTTACA TTCCTGACTA CCGATTGGCT 
GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT 
CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA 
AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA 
CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA 
CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA 
CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT 
CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA 
CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG 
GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG 
AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGA 
AAAGCCTTTA CCTCCTCTCA AAGACCTCGA CCTACCAGCC CATTTTCTAC TGACAGTAAC 
ACCAGTGCAG CCCTGAGTCA AAGTCAGAGG CCTCGGCCCA CTAAAAAACA CAAGGGAGGG 
CGGATGGACC AACAACCAGC ATTGCCTCAT CGAAGGGAAG GAATGACAGA TGAGGAGGCC 
TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA TCTCCAGGTG GCCACAGCTC ATCAGGAACA 
GCTTCTTCTA AGGGATCCAC TGGACCTAGG AAAACCGAGG TGTTGAGAGC AGGCCACCAG 
CGCAATGCCA GCGACCTTCT TGACATAGGA TATATGGGCT CCAACAGTCA AGGACAGTTT 
ACAGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017007000.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862489.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X19, mRNA.

Price & Availability↑

CloneID	OHu94474
Clone ID Related Accession (Same CDS sequence)	XM_017007000.1
Accession Version	XM_017007000.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4263bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT 
AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA 
GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT 
TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA 
GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC 
TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG 
GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT 
TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG 
GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT 
GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT 
CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT 
GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC 
CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC 
TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC 
CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA 
CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG AGGAGGCCTT GGTGCCCTAT 
AGCAAGCCCA GTTTCCCATC TCCAGGTGGC CACAGCTCAT CAGGAACAGC TTCTTCTAAG 
GGATCCACTG GACCTAGGAA AACCGAGGTG TTGAGAGCAG GCCACCAGCG CAATGCCAGC 
GACCTTCTTG ACATAGGATA TATGGGCTCC AACAGTCAAG GACAGTTTAC AGGTGAATTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862489.1
CDS	692..4954
Translation	MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017007000.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007000.1

ORF Insert Sequence:

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT 
AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA 
GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT 
TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA 
GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC 
TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG 
GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT 
TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG 
GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT 
GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT 
CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT 
GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC 
CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC 
TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC 
CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA 
CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG AGGAGGCCTT GGTGCCCTAT 
AGCAAGCCCA GTTTCCCATC TCCAGGTGGC CACAGCTCAT CAGGAACAGC TTCTTCTAAG 
GGATCCACTG GACCTAGGAA AACCGAGGTG TTGAGAGCAG GCCACCAGCG CAATGCCAGC 
GACCTTCTTG ACATAGGATA TATGGGCTCC AACAGTCAAG GACAGTTTAC AGGTGAATTA 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017007001.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862490.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X21, mRNA.

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CloneID	OHu94475
Clone ID Related Accession (Same CDS sequence)	XM_017007001.1
Accession Version	XM_017007001.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4212bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG 
AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT 
CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA 
CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC 
TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT 
GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT 
ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA 
AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT 
CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA 
ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC 
CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA 
GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT 
AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA 
GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG 
GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA 
ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC 
CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG 
TTTACAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862490.1
CDS	400..4611
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_017007001.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007001.1

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG 
AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT 
CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA 
CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC 
TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT 
GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT 
ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA 
AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT 
CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA 
ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC 
CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA 
GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT 
AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA 
GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG 
GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA 
ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC 
CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG 
TTTACAGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017007002.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862491.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X22, mRNA.

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CloneID	OHu94476
Clone ID Related Accession (Same CDS sequence)	XM_017007002.1
Accession Version	XM_017007002.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4209bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA 
AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC 
CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA 
AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA 
GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC 
GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT 
CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT 
GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA 
CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGAAAAGCC 
TTTACCTCCT CTCAAAGACC TCGACCTACC AGCCCATTTT CTACTGACAG TAACACCAGT 
GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG AGGGCGGATG 
GACCAACAAC CAGCATTGCC TCATCGAAGG GAAGGAATGA CAGATGAGGA GGCCTTGGTG 
CCCTATAGCA AGCCCAGTTT CCCATCTCCA GGTGGCCACA GCTCATCAGG AACAGCTTCT 
TCTAAGGGAT CCACTGGACC TAGGAAAACC GAGGTGTTGA GAGCAGGCCA CCAGCGCAAT 
GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCCAACA GTCAAGGACA GTTTACAGGT 
GAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862491.1
CDS	400..4608
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017007002.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007002.1

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA 
AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC 
CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA 
AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA 
GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC 
GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT 
CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT 
GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA 
CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGAAAAGCC 
TTTACCTCCT CTCAAAGACC TCGACCTACC AGCCCATTTT CTACTGACAG TAACACCAGT 
GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG AGGGCGGATG 
GACCAACAAC CAGCATTGCC TCATCGAAGG GAAGGAATGA CAGATGAGGA GGCCTTGGTG 
CCCTATAGCA AGCCCAGTTT CCCATCTCCA GGTGGCCACA GCTCATCAGG AACAGCTTCT 
TCTAAGGGAT CCACTGGACC TAGGAAAACC GAGGTGTTGA GAGCAGGCCA CCAGCGCAAT 
GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCCAACA GTCAAGGACA GTTTACAGGT 
GAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017007003.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862492.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X23, mRNA.

Price & Availability↑

CloneID	OHu94477
Clone ID Related Accession (Same CDS sequence)	XM_017007003.1
Accession Version	XM_017007003.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCCCCC ACAGTTTGTG 
GTTCGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TGACATTTCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCAAACCAAC CCCAGCAGCC CAACAGTAGA TGCTCAGTGT CACCAACTGG AGACCTCACA 
ATCACCAACA TTCAACGTTC CGACGCGGGT TACTACATCT GCCAGGCTTT AACTGTGGCA 
GGAAGCATTT TAGCAAAAGC TCAACTGGAG GTTACTGATG TTTTGACAGA TAGACCTCCA 
CCTATAATTC TACAAGGCCC AGCCAACCAA ACGCTGGCAG TGGATGGTAC AGCGTTACTG 
AAATGTAAAG CCACTGGTGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT 
TTTCCGGGTA GAGATCCAAG AGCAACAATT CAAGAGCAAG GCACACTGCA GATTAAGAAT 
TTACGGATTT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCAAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTGACAGAG TCTGGAGCAA CAATCAGTAA AAACTATGAT 
TTAAGTGACC TGCCAGGGCC ACCATCCAAA CCGCAGGTCA CTGATGTTAC TAAGAACAGT 
GTCACCTTGT CCTGGCAGCC AGGTACCCCT GGAACCCTTC CAGCAAGTGC ATATATCATT 
GAGGCTTTCA GCCAATCAGT GAGCAACAGC TGGCAGACCG TGGCAAACCA TGTAAAGACC 
ACCCTCTATA CTGTAAGAGG ACTGCGGCCC AATACAATCT ACTTATTCAT GGTCAGAGCG 
ATCAACCCCC AAGGTCTCAG TGACCCAAGT CCCATGTCAG ATCCTGTGCG CACACAAGAT 
ATCAGCCCAC CAGCACAAGG AGTGGACCAC AGGCAAGTGC AGAAAGAGCT AGGAGATGTC 
CTTGTCCGTC TTCATAATCC AGTTGTGCTG ACTCCCACCA CGGTTCAGGT CACATGGACG 
GTTGATCGCC AACCCCAGTT TATCCAAGGC TACCGAGTGA TGTATCGTCA GACTTCAGGT 
CTGCAGGCGA CATCTTCGTG GCAGAATTTA GATGCCAAAG TCCCGACTGA ACGAAGTGCT 
GTCTTAGTCA ACCTGAAAAA GGGGGTGACT TATGAAATTA AAGTACGGCC ATATTTTAAT 
GAGTTCCAAG GAATGGATAG TGAATCTAAA ACGGTTCGTA CTACTGAAGA AGCCCCAAGT 
GCCCCACCAC AGTCTGTCAC TGTACTGACA GTTGGAAGCT ACAATAGCAC AAGTATTAGT 
GTTTCCTGGG ATCCTCCTCC TCCAGATCAC CAGAATGGAA TTATCCAAGA ATACAAGATC 
TGGTGTCTAG GAAATGAAAC GCGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGG 
TCCGTAATAA TTGGTGGATT ATTCCCAGGT ATTCAATACC GGGTAGAGGT TGCAGCTAGT 
ACCAGTGCAG GGGTTGGAGT AAAGAGTGAG CCACAGCCAA TAATAATCGG GAGACGCAAT 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTGGTGAAG 
CAACCAGCCT TTATAGCTGG TATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATATGGTTGT ATTGGCGAAG AAAGAAGAGG AAGGGACTCA GTAATTATGC TGTTACGTTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGAAGCCGTC CAGGTCTTCT CAATGCTGGT 
GATCCCAGCT ATCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGCTTGCC AGTAAATAAT 
AGCAACAGTG GCCCAAATGA GATTGGAAAT TTTGGCCGTG GAGATGTGCT GCCACCAGTT 
CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGTAGCATT 
GACTTCACTA CCAAAACCAG TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT 
GCCACGACAC AGATCTTGCA TTCCAACAGC ATACATGAAT TGGCTGTCGA TCTGCCTGAT 
CCACAATGGA AAAGCTCAAT TCAGCAAAAA ACAGATCTGA TGGGATTTGG TTATTCTCTA 
CCTGATCAGA ACAAAGGTAA CAATGGTGGG AAAGGTGGAA AAAAGAAGAA AAATAAAAAC 
TCTTCTAAAC CACAGAAAAA CAATGGATCC ACTTGGGCCA ATGTCCCTCT ACCTCCCCCC 
CCAGTCCAGC CCCTTCCTGG CACGGAGCTG GAACACTATG CAGTGGAACA ACAAGAAAAT 
GGCTATGACA GTGATAGCTG GTGCCCACCA TTGCCAGTAC AAACTTACTT ACACCAAGGT 
CTGGAAGATG AACTGGAAGA AGATGATGAT AGGGTCCCAA CACCTCCTGT TCGAGGCGTG 
GCTTCTTCTC CTGCTATCTC CTTTGGACAG CAGTCCACTG CAACTCTTAC TCCATCCCCA 
CGGGAAGAGA TGCAACCCAT GCTGCAGGCT CACCTGGATG AGTTGACAAG AGCCTATCAG 
TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA ATCAACCTCC ACAGCCTCCA 
GTTCCACCGT TAGGTTATGT GTCTGGAGCC TTGATTTCTG ATTTGGAAAC GGATGTTGCA 
GATGATGATG CCGACGACGA AGAGGAAGCT TTAGAAATCC CCAGGCCCCT GAGAGCACTG 
GACCAGACTC CTGGATCCAG CATGGACAAT CTAGACAGCT CTGTGACAGG AAAAGCCTTT 
ACCTCCTCTC AAAGACCTCG ACCTACCAGC CCATTTTCTA CTGACAGTAA CACCAGTGCA 
GCCCTGAGTC AAAGTCAGAG GCCTCGGCCC ACTAAAAAAC ACAAGGGAGG GCGGATGGAC 
CAACAACCAG CATTGCCTCA TCGAAGGGAA GGAATGACAG ATGAGGAGGC CTTGGTGCCC 
TATAGCAAGC CCAGTTTCCC ATCTCCAGGT GGCCACAGCT CATCAGGAAC AGCTTCTTCT 
AAGGGATCCA CTGGACCTAG GAAAACCGAG GTGTTGAGAG CAGGCCACCA GCGCAATGCC 
AGCGACCTTC TTGACATAGG ATATATGGGC TCCAACAGTC AAGGACAGTT TACAGGTGAA 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862492.1
CDS	400..4605
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017007003.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007003.1

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCCCCC ACAGTTTGTG 
GTTCGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TGACATTTCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCAAACCAAC CCCAGCAGCC CAACAGTAGA TGCTCAGTGT CACCAACTGG AGACCTCACA 
ATCACCAACA TTCAACGTTC CGACGCGGGT TACTACATCT GCCAGGCTTT AACTGTGGCA 
GGAAGCATTT TAGCAAAAGC TCAACTGGAG GTTACTGATG TTTTGACAGA TAGACCTCCA 
CCTATAATTC TACAAGGCCC AGCCAACCAA ACGCTGGCAG TGGATGGTAC AGCGTTACTG 
AAATGTAAAG CCACTGGTGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT 
TTTCCGGGTA GAGATCCAAG AGCAACAATT CAAGAGCAAG GCACACTGCA GATTAAGAAT 
TTACGGATTT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCAAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTGACAGAG TCTGGAGCAA CAATCAGTAA AAACTATGAT 
TTAAGTGACC TGCCAGGGCC ACCATCCAAA CCGCAGGTCA CTGATGTTAC TAAGAACAGT 
GTCACCTTGT CCTGGCAGCC AGGTACCCCT GGAACCCTTC CAGCAAGTGC ATATATCATT 
GAGGCTTTCA GCCAATCAGT GAGCAACAGC TGGCAGACCG TGGCAAACCA TGTAAAGACC 
ACCCTCTATA CTGTAAGAGG ACTGCGGCCC AATACAATCT ACTTATTCAT GGTCAGAGCG 
ATCAACCCCC AAGGTCTCAG TGACCCAAGT CCCATGTCAG ATCCTGTGCG CACACAAGAT 
ATCAGCCCAC CAGCACAAGG AGTGGACCAC AGGCAAGTGC AGAAAGAGCT AGGAGATGTC 
CTTGTCCGTC TTCATAATCC AGTTGTGCTG ACTCCCACCA CGGTTCAGGT CACATGGACG 
GTTGATCGCC AACCCCAGTT TATCCAAGGC TACCGAGTGA TGTATCGTCA GACTTCAGGT 
CTGCAGGCGA CATCTTCGTG GCAGAATTTA GATGCCAAAG TCCCGACTGA ACGAAGTGCT 
GTCTTAGTCA ACCTGAAAAA GGGGGTGACT TATGAAATTA AAGTACGGCC ATATTTTAAT 
GAGTTCCAAG GAATGGATAG TGAATCTAAA ACGGTTCGTA CTACTGAAGA AGCCCCAAGT 
GCCCCACCAC AGTCTGTCAC TGTACTGACA GTTGGAAGCT ACAATAGCAC AAGTATTAGT 
GTTTCCTGGG ATCCTCCTCC TCCAGATCAC CAGAATGGAA TTATCCAAGA ATACAAGATC 
TGGTGTCTAG GAAATGAAAC GCGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGG 
TCCGTAATAA TTGGTGGATT ATTCCCAGGT ATTCAATACC GGGTAGAGGT TGCAGCTAGT 
ACCAGTGCAG GGGTTGGAGT AAAGAGTGAG CCACAGCCAA TAATAATCGG GAGACGCAAT 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTGGTGAAG 
CAACCAGCCT TTATAGCTGG TATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATATGGTTGT ATTGGCGAAG AAAGAAGAGG AAGGGACTCA GTAATTATGC TGTTACGTTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGAAGCCGTC CAGGTCTTCT CAATGCTGGT 
GATCCCAGCT ATCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGCTTGCC AGTAAATAAT 
AGCAACAGTG GCCCAAATGA GATTGGAAAT TTTGGCCGTG GAGATGTGCT GCCACCAGTT 
CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGTAGCATT 
GACTTCACTA CCAAAACCAG TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT 
GCCACGACAC AGATCTTGCA TTCCAACAGC ATACATGAAT TGGCTGTCGA TCTGCCTGAT 
CCACAATGGA AAAGCTCAAT TCAGCAAAAA ACAGATCTGA TGGGATTTGG TTATTCTCTA 
CCTGATCAGA ACAAAGGTAA CAATGGTGGG AAAGGTGGAA AAAAGAAGAA AAATAAAAAC 
TCTTCTAAAC CACAGAAAAA CAATGGATCC ACTTGGGCCA ATGTCCCTCT ACCTCCCCCC 
CCAGTCCAGC CCCTTCCTGG CACGGAGCTG GAACACTATG CAGTGGAACA ACAAGAAAAT 
GGCTATGACA GTGATAGCTG GTGCCCACCA TTGCCAGTAC AAACTTACTT ACACCAAGGT 
CTGGAAGATG AACTGGAAGA AGATGATGAT AGGGTCCCAA CACCTCCTGT TCGAGGCGTG 
GCTTCTTCTC CTGCTATCTC CTTTGGACAG CAGTCCACTG CAACTCTTAC TCCATCCCCA 
CGGGAAGAGA TGCAACCCAT GCTGCAGGCT CACCTGGATG AGTTGACAAG AGCCTATCAG 
TTTGATATAG CAAAACAAAC ATGGCACATT CAAAGCAATA ATCAACCTCC ACAGCCTCCA 
GTTCCACCGT TAGGTTATGT GTCTGGAGCC TTGATTTCTG ATTTGGAAAC GGATGTTGCA 
GATGATGATG CCGACGACGA AGAGGAAGCT TTAGAAATCC CCAGGCCCCT GAGAGCACTG 
GACCAGACTC CTGGATCCAG CATGGACAAT CTAGACAGCT CTGTGACAGG AAAAGCCTTT 
ACCTCCTCTC AAAGACCTCG ACCTACCAGC CCATTTTCTA CTGACAGTAA CACCAGTGCA 
GCCCTGAGTC AAAGTCAGAG GCCTCGGCCC ACTAAAAAAC ACAAGGGAGG GCGGATGGAC 
CAACAACCAG CATTGCCTCA TCGAAGGGAA GGAATGACAG ATGAGGAGGC CTTGGTGCCC 
TATAGCAAGC CCAGTTTCCC ATCTCCAGGT GGCCACAGCT CATCAGGAAC AGCTTCTTCT 
AAGGGATCCA CTGGACCTAG GAAAACCGAG GTGTTGAGAG CAGGCCACCA GCGCAATGCC 
AGCGACCTTC TTGACATAGG ATATATGGGC TCCAACAGTC AAGGACAGTT TACAGGTGAA 
TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017007004.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862493.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X24, mRNA.

Price & Availability↑

CloneID	OHu94478
Clone ID Related Accession (Same CDS sequence)	XM_017007004.1
Accession Version	XM_017007004.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA 
CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG 
CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC 
AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT 
GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT 
CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG 
TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT 
GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT 
CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT 
CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT 
CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA 
GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG 
CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC 
ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT 
CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862493.1
CDS	300..4496
Translation	MARRHERVTRRMWTWAPGLLMMTVVFWGHQGNGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017007004.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007004.1

ORF Insert Sequence:

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA 
CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG 
CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC 
AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT 
GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT 
CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG 
TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT 
GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT 
CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT 
CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT 
CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA 
GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG 
CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC 
ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT 
CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017007005.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862494.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X25, mRNA.

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CloneID	OHu94479
Clone ID Related Accession (Same CDS sequence)	XM_017007005.1
Accession Version	XM_017007005.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4191bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA 
CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG 
CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC 
AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT 
GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT 
CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG 
TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT 
GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT 
CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT 
CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT 
CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA 
GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG 
CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC 
ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT 
CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862494.1
CDS	300..4490
Translation	MARRHERVTRRMWTWAPGLLMMTVVFWGHQGNGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_017007005.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007005.1

ORF Insert Sequence:

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA 
CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG 
CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC 
AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT 
GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT 
CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG 
TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT 
GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT 
CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT 
CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT 
CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA 
GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG 
CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC 
ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT 
CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017007006.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862495.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X26, mRNA.

Price & Availability↑

CloneID	OHu94480
Clone ID Related Accession (Same CDS sequence)	XM_017007006.1
Accession Version	XM_017007006.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4140bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TGGGAAAGGT GGAAAAAAGA AGAAAAATAA AAACTCTTCT 
AAACCACAGA AAAACAATGG ATCCACTTGG GCCAATGTCC CTCTACCTCC CCCCCCAGTC 
CAGCCCCTTC CTGGCACGGA GCTGGAACAC TATGCAGTGG AACAACAAGA AAATGGCTAT 
GACAGTGATA GCTGGTGCCC ACCATTGCCA GTACAAACTT ACTTACACCA AGGTCTGGAA 
GATGAACTGG AAGAAGATGA TGATAGGGTC CCAACACCTC CTGTTCGAGG CGTGGCTTCT 
TCTCCTGCTA TCTCCTTTGG ACAGCAGTCC ACTGCAACTC TTACTCCATC CCCACGGGAA 
GAGATGCAAC CCATGCTGCA GGCTCACCTG GATGAGTTGA CAAGAGCCTA TCAGTTTGAT 
ATAGCAAAAC AAACATGGCA CATTCAAAGC AATAATCAAC CTCCACAGCC TCCAGTTCCA 
CCGTTAGGTT ATGTGTCTGG AGCCTTGATT TCTGATTTGG AAACGGATGT TGCAGATGAT 
GATGCCGACG ACGAAGAGGA AGCTTTAGAA ATCCCCAGGC CCCTGAGAGC ACTGGACCAG 
ACTCCTGGAT CCAGCATGGA CAATCTAGAC AGCTCTGTGA CAGGAAAAGC CTTTACCTCC 
TCTCAAAGAC CTCGACCTAC CAGCCCATTT TCTACTGACA GTAACACCAG TGCAGCCCTG 
AGTCAAAGTC AGAGGCCTCG GCCCACTAAA AAACACAAGG GAGGGCGGAT GGACCAACAA 
CCAGCATTGC CTCATCGAAG GGAAGGAATG ACAGATGAGG AGGCCTTGGT GCCCTATAGC 
AAGCCCAGTT TCCCATCTCC AGGTGGCCAC AGCTCATCAG GAACAGCTTC TTCTAAGGGA 
TCCACTGGAC CTAGGAAAAC CGAGGTGTTG AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC 
CTTCTTGACA TAGGATATAT GGGCTCCAAC AGTCAAGGAC AGTTTACAGG TGAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862495.1
CDS	692..4831
Translation	MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017007006.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017007006.1

ORF Insert Sequence:

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TGGGAAAGGT GGAAAAAAGA AGAAAAATAA AAACTCTTCT 
AAACCACAGA AAAACAATGG ATCCACTTGG GCCAATGTCC CTCTACCTCC CCCCCCAGTC 
CAGCCCCTTC CTGGCACGGA GCTGGAACAC TATGCAGTGG AACAACAAGA AAATGGCTAT 
GACAGTGATA GCTGGTGCCC ACCATTGCCA GTACAAACTT ACTTACACCA AGGTCTGGAA 
GATGAACTGG AAGAAGATGA TGATAGGGTC CCAACACCTC CTGTTCGAGG CGTGGCTTCT 
TCTCCTGCTA TCTCCTTTGG ACAGCAGTCC ACTGCAACTC TTACTCCATC CCCACGGGAA 
GAGATGCAAC CCATGCTGCA GGCTCACCTG GATGAGTTGA CAAGAGCCTA TCAGTTTGAT 
ATAGCAAAAC AAACATGGCA CATTCAAAGC AATAATCAAC CTCCACAGCC TCCAGTTCCA 
CCGTTAGGTT ATGTGTCTGG AGCCTTGATT TCTGATTTGG AAACGGATGT TGCAGATGAT 
GATGCCGACG ACGAAGAGGA AGCTTTAGAA ATCCCCAGGC CCCTGAGAGC ACTGGACCAG 
ACTCCTGGAT CCAGCATGGA CAATCTAGAC AGCTCTGTGA CAGGAAAAGC CTTTACCTCC 
TCTCAAAGAC CTCGACCTAC CAGCCCATTT TCTACTGACA GTAACACCAG TGCAGCCCTG 
AGTCAAAGTC AGAGGCCTCG GCCCACTAAA AAACACAAGG GAGGGCGGAT GGACCAACAA 
CCAGCATTGC CTCATCGAAG GGAAGGAATG ACAGATGAGG AGGCCTTGGT GCCCTATAGC 
AAGCCCAGTT TCCCATCTCC AGGTGGCCAC AGCTCATCAG GAACAGCTTC TTCTAAGGGA 
TCCACTGGAC CTAGGAAAAC CGAGGTGTTG AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC 
CTTCTTGACA TAGGATATAT GGGCTCCAAC AGTCAAGGAC AGTTTACAGG TGAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006986.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862475.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu94460
Clone ID Related Accession (Same CDS sequence)	XM_017006986.1
Accession Version	XM_017006986.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4731bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGCC TTACTTTACA TTCCTGACTA CCGATTGGCT 
GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT 
CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA 
AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA 
CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA 
CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA 
CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT 
CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA 
CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG 
GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG 
AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGT 
TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA 
TCTAGTGTAG GTAGCTCCTC AGATGGCTCT ATCTTTGCCA GCGGCAGTTT TGCACAAGCA 
CTGGTGGCAG CAGCAGATAA AGCTGGTTTT AGGCTGGATG GAACCAGCCT TACAAGAACA 
GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT 
AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA 
GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGATCTT 
CCACCACCAC CAGATCCCCC GCCAGGTCAG GGTTTAAGGC AGCAAATAGG CCCGAGCCAG 
CAGGCTGGTA ACGTGGAAAA CTCAGCAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAT 
GCATCCAGCT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAGAAC CTCCCTAGAG 
TGGCAGCGAC AAACCCAGGA ATGGATAAGC TCCACAGAAC GACAAGAAGA TATACGGAAA 
GCCCCACACA AACAAGGTGT CGGATCAGAG GAGGCCTTGG TGCCCTATAG CAAGCCCAGT 
TTCCCATCTC CAGGTGGCCA CAGCTCATCA GGAACAGCTT CTTCTAAGGG ATCCACTGGA 
CCTAGGAAAA CCGAGGTGTT GAGAGCAGGC CACCAGCGCA ATGCCAGCGA CCTTCTTGAC 
ATAGGATATA TGGGCTCCAA CAGTCAAGGA CAGTTTACAG GTGAATTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862475.1
CDS	692..5422
Translation	MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAQALVAAADKAGFRLDGTSLTRTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDDLPPPPDPPPGQGLRQQIGPSQQAGNVENSAERKGSSLERQHASSLEDTKSSLDCPARTSLEWQRQTQEWISSTERQEDIRKAPHKQGVGSEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017006986.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006986.1

ORF Insert Sequence:

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGCC TTACTTTACA TTCCTGACTA CCGATTGGCT 
GAGGGATTGT CTAATAGAAT GCCACACAAC CAGTCTCAGG ATTTCAGCAC CACCAGCTCT 
CACAACAGCT CAGAAAGGAG TGGCAGTCTC TCAGGTGGGA AAGGTGGAAA AAAGAAGAAA 
AATAAAAACT CTTCTAAACC ACAGAAAAAC AATGGATCCA CTTGGGCCAA TGTCCCTCTA 
CCTCCCCCCC CAGTCCAGCC CCTTCCTGGC ACGGAGCTGG AACACTATGC AGTGGAACAA 
CAAGAAAATG GCTATGACAG TGATAGCTGG TGCCCACCAT TGCCAGTACA AACTTACTTA 
CACCAAGGTC TGGAAGATGA ACTGGAAGAA GATGATGATA GGGTCCCAAC ACCTCCTGTT 
CGAGGCGTGG CTTCTTCTCC TGCTATCTCC TTTGGACAGC AGTCCACTGC AACTCTTACT 
CCATCCCCAC GGGAAGAGAT GCAACCCATG CTGCAGGCTC ACCTGGATGA GTTGACAAGA 
GCCTATCAGT TTGATATAGC AAAACAAACA TGGCACATTC AAAGCAATAA TCAACCTCCA 
CAGCCTCCAG TTCCACCGTT AGGTTATGTG TCTGGAGCCT TGATTTCTGA TTTGGAAACG 
GATGTTGCAG ATGATGATGC CGACGACGAA GAGGAAGCTT TAGAAATCCC CAGGCCCCTG 
AGAGCACTGG ACCAGACTCC TGGATCCAGC ATGGACAATC TAGACAGCTC TGTGACAGGT 
TCAATGGTAA ATGGATGGGG CTCTGCATCT GATGAGGATC GTAACTTTTC TAGTCATAGA 
TCTAGTGTAG GTAGCTCCTC AGATGGCTCT ATCTTTGCCA GCGGCAGTTT TGCACAAGCA 
CTGGTGGCAG CAGCAGATAA AGCTGGTTTT AGGCTGGATG GAACCAGCCT TACAAGAACA 
GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT 
AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA 
GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGATCTT 
CCACCACCAC CAGATCCCCC GCCAGGTCAG GGTTTAAGGC AGCAAATAGG CCCGAGCCAG 
CAGGCTGGTA ACGTGGAAAA CTCAGCAGAG AGAAAAGGAA GCTCTCTAGA GAGACAACAT 
GCATCCAGCT TAGAAGACAC AAAGAGCTCA TTGGATTGTC CAGCTAGAAC CTCCCTAGAG 
TGGCAGCGAC AAACCCAGGA ATGGATAAGC TCCACAGAAC GACAAGAAGA TATACGGAAA 
GCCCCACACA AACAAGGTGT CGGATCAGAG GAGGCCTTGG TGCCCTATAG CAAGCCCAGT 
TTCCCATCTC CAGGTGGCCA CAGCTCATCA GGAACAGCTT CTTCTAAGGG ATCCACTGGA 
CCTAGGAAAA CCGAGGTGTT GAGAGCAGGC CACCAGCGCA ATGCCAGCGA CCTTCTTGAC 
ATAGGATATA TGGGCTCCAA CAGTCAAGGA CAGTTTACAG GTGAATTATA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006987.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862476.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X4, mRNA.

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CloneID	OHu94461
Clone ID Related Accession (Same CDS sequence)	XM_017006987.1
Accession Version	XM_017006987.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4665bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA 
AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC 
CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA 
AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA 
GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC 
GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT 
CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT 
GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA 
CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGTTCAATG 
GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT 
GTAGGTAGCT CCTCAGATGG CTCTATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG 
GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGAAAA 
GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC 
AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG 
ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA 
CCACCAGATC CCCCGCCAGG TCAGGGTTTA AGGCAGCAAA TAGGCCCGAG CCAGCAGGCT 
GGTAACGTGG AAAACTCAGC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACATGCATCC 
AGCTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA GAACCTCCCT AGAGTGGCAG 
CGACAAACCC AGGAATGGAT AAGCTCCACA GAACGACAAG AAGATATACG GAAAGCCCCA 
CACAAACAAG GTGTCGGATC AGAGGAGGCC TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA 
TCTCCAGGTG GCCACAGCTC ATCAGGAACA GCTTCTTCTA AGGGATCCAC TGGACCTAGG 
AAAACCGAGG TGTTGAGAGC AGGCCACCAG CGCAATGCCA GCGACCTTCT TGACATAGGA 
TATATGGGCT CCAACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862476.1
CDS	400..5064
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAQALVAAADKAGFRLDGTSLTRTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDDLPPPPDPPPGQGLRQQIGPSQQAGNVENSAERKGSSLERQHASSLEDTKSSLDCPARTSLEWQRQTQEWISSTERQEDIRKAPHKQGVGSEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017006987.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006987.1

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA 
AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC 
CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA 
AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA 
GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC 
GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT 
CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT 
GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA 
CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGTTCAATG 
GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT 
GTAGGTAGCT CCTCAGATGG CTCTATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG 
GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGAAAA 
GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC 
AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG 
ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA 
CCACCAGATC CCCCGCCAGG TCAGGGTTTA AGGCAGCAAA TAGGCCCGAG CCAGCAGGCT 
GGTAACGTGG AAAACTCAGC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACATGCATCC 
AGCTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA GAACCTCCCT AGAGTGGCAG 
CGACAAACCC AGGAATGGAT AAGCTCCACA GAACGACAAG AAGATATACG GAAAGCCCCA 
CACAAACAAG GTGTCGGATC AGAGGAGGCC TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA 
TCTCCAGGTG GCCACAGCTC ATCAGGAACA GCTTCTTCTA AGGGATCCAC TGGACCTAGG 
AAAACCGAGG TGTTGAGAGC AGGCCACCAG CGCAATGCCA GCGACCTTCT TGACATAGGA 
TATATGGGCT CCAACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006988.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862477.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X6, mRNA.

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CloneID	OHu94462
Clone ID Related Accession (Same CDS sequence)	XM_017006988.1
Accession Version	XM_017006988.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4611bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT 
GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA 
GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA 
ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA 
CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA 
ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG 
TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT 
CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT 
TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC 
AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT 
GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT 
GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC 
AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT 
GATCTTCCAC CACCACCAGA TCCCCCGCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG 
AGCCAGCAGG CTGGTAACGT GGAAAACTCA GCAGAGAGAA AAGGAAGCTC TCTAGAGAGA 
CAACATGCAT CCAGCTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACCTCC 
CTAGAGTGGC AGCGACAAAC CCAGGAATGG ATAAGCTCCA CAGAACGACA AGAAGATATA 
CGGAAAGCCC CACACAAACA AGGTGTCGGA TCAGAGGAGG CCTTGGTGCC CTATAGCAAG 
CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC 
ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT 
CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862477.1
CDS	400..5010
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDDLPPPPDPPPGQGLRQQIGPSQQAGNVENSAERKGSSLERQHASSLEDTKSSLDCPARTSLEWQRQTQEWISSTERQEDIRKAPHKQGVGSEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_017006988.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006988.1

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT 
GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA 
GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA 
ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA 
CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA 
ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG 
TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT 
CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT 
TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC 
AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT 
GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT 
GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC 
AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT 
GATCTTCCAC CACCACCAGA TCCCCCGCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG 
AGCCAGCAGG CTGGTAACGT GGAAAACTCA GCAGAGAGAA AAGGAAGCTC TCTAGAGAGA 
CAACATGCAT CCAGCTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACCTCC 
CTAGAGTGGC AGCGACAAAC CCAGGAATGG ATAAGCTCCA CAGAACGACA AGAAGATATA 
CGGAAAGCCC CACACAAACA AGGTGTCGGA TCAGAGGAGG CCTTGGTGCC CTATAGCAAG 
CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC 
ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT 
CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006989.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862478.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X7, mRNA.

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CloneID	OHu94463
Clone ID Related Accession (Same CDS sequence)	XM_017006989.1
Accession Version	XM_017006989.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4605bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCCCCC ACAGTTTGTG 
GTTCGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TGACATTTCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCAAACCAAC CCCAGCAGCC CAACAGTAGA TGCTCAGTGT CACCAACTGG AGACCTCACA 
ATCACCAACA TTCAACGTTC CGACGCGGGT TACTACATCT GCCAGGCTTT AACTGTGGCA 
GGAAGCATTT TAGCAAAAGC TCAACTGGAG GTTACTGATG TTTTGACAGA TAGACCTCCA 
CCTATAATTC TACAAGGCCC AGCCAACCAA ACGCTGGCAG TGGATGGTAC AGCGTTACTG 
AAATGTAAAG CCACTGGTGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT 
TTTCCGGGTA GAGATCCAAG AGCAACAATT CAAGAGCAAG GCACACTGCA GATTAAGAAT 
TTACGGATTT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCAAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTGACAGAG TCTGGAGCAA CAATCAGTAA AAACTATGAT 
TTAAGTGACC TGCCAGGGCC ACCATCCAAA CCGCAGGTCA CTGATGTTAC TAAGAACAGT 
GTCACCTTGT CCTGGCAGCC AGGTACCCCT GGAACCCTTC CAGCAAGTGC ATATATCATT 
GAGGCTTTCA GCCAATCAGT GAGCAACAGC TGGCAGACCG TGGCAAACCA TGTAAAGACC 
ACCCTCTATA CTGTAAGAGG ACTGCGGCCC AATACAATCT ACTTATTCAT GGTCAGAGCG 
ATCAACCCCC AAGGTCTCAG TGACCCAAGT CCCATGTCAG ATCCTGTGCG CACACAAGAT 
ATCAGCCCAC CAGCACAAGG AGTGGACCAC AGGCAAGTGC AGAAAGAGCT AGGAGATGTC 
CTTGTCCGTC TTCATAATCC AGTTGTGCTG ACTCCCACCA CGGTTCAGGT CACATGGACG 
GTTGATCGCC AACCCCAGTT TATCCAAGGC TACCGAGTGA TGTATCGTCA GACTTCAGGT 
CTGCAGGCGA CATCTTCGTG GCAGAATTTA GATGCCAAAG TCCCGACTGA ACGAAGTGCT 
GTCTTAGTCA ACCTGAAAAA GGGGGTGACT TATGAAATTA AAGTACGGCC ATATTTTAAT 
GAGTTCCAAG GAATGGATAG TGAATCTAAA ACGGTTCGTA CTACTGAAGA AGCCCCAAGT 
GCCCCACCAC AGTCTGTCAC TGTACTGACA GTTGGAAGCT ACAATAGCAC AAGTATTAGT 
GTTTCCTGGG ATCCTCCTCC TCCAGATCAC CAGAATGGAA TTATCCAAGA ATACAAGATC 
TGGTGTCTAG GAAATGAAAC GCGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGG 
TCCGTAATAA TTGGTGGATT ATTCCCAGGT ATTCAATACC GGGTAGAGGT TGCAGCTAGT 
ACCAGTGCAG GGGTTGGAGT AAAGAGTGAG CCACAGCCAA TAATAATCGG GAGACGCAAT 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTGGTGAAG 
CAACCAGCCT TTATAGCTGG TATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATATGGTTGT ATTGGCGAAG AAAGAAGAGG AAGGGACTCA GTAATTATGC TGTTACGTTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGAAGCCGTC CAGGTCTTCT CAATGCTGGT 
GATCCCAGCT ATCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGCTTGCC AGTAAATAAT 
AGCAACAGTG GCCCAAATGA GATTGGAAAT TTTGGCCGTG GAGATGTGCT GCCACCAGTT 
CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGTAGCATT 
GACTTCACTA CCAAAACCAG TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT 
GCCACGACAC AGATCTTGCA TTCCAACAGC ATACATGAAT TGGCTGTCGA TCTGCCTGAT 
CCACAATGGA AAAGCTCAAT TCAGCAAAAA ACAGATCTGA TGGGATTTGG TTATTCTCTA 
CCTGATCAGA ACAAAGGTAA CAATGCCTTA CTTTACATTC CTGACTACCG ATTGGCTGAG 
GGATTGTCTA ATAGAATGCC ACACAACCAG TCTCAGGATT TCAGCACCAC CAGCTCTCAC 
AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAAAT 
AAAAACTCTT CTAAACCACA GAAAAACAAT GGATCCACTT GGGCCAATGT CCCTCTACCT 
CCCCCCCCAG TCCAGCCCCT TCCTGGCACG GAGCTGGAAC ACTATGCAGT GGAACAACAA 
GAAAATGGCT ATGACAGTGA TAGCTGGTGC CCACCATTGC CAGTACAAAC TTACTTACAC 
CAAGGTCTGG AAGATGAACT GGAAGAAGAT GATGATAGGG TCCCAACACC TCCTGTTCGA 
GGCGTGGCTT CTTCTCCTGC TATCTCCTTT GGACAGCAGT CCACTGCAAC TCTTACTCCA 
TCCCCACGGG AAGAGATGCA ACCCATGCTG CAGGCTCACC TGGATGAGTT GACAAGAGCC 
TATCAGTTTG ATATAGCAAA ACAAACATGG CACATTCAAA GCAATAATCA ACCTCCACAG 
CCTCCAGTTC CACCGTTAGG TTATGTGTCT GGAGCCTTGA TTTCTGATTT GGAAACGGAT 
GTTGCAGATG ATGATGCCGA CGACGAAGAG GAAGCTTTAG AAATCCCCAG GCCCCTGAGA 
GCACTGGACC AGACTCCTGG ATCCAGCATG GACAATCTAG ACAGCTCTGT GACAGGAAAA 
GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC 
AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG 
ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA 
CCACCAGATC CCCCGCCAGG TCAGGGTTTA AGGCAGCAAA TAGGCCCGAG CCAGCAGGCT 
GGTAACGTGG AAAACTCAGC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACATGCATCC 
AGCTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA GAACCTCCCT AGAGTGGCAG 
CGACAAACCC AGGAATGGAT AAGCTCCACA GAACGACAAG AAGATATACG GAAAGCCCCA 
CACAAACAAG GTGTCGGATC AGAGGAGGCC TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA 
TCTCCAGGTG GCCACAGCTC ATCAGGAACA GCTTCTTCTA AGGGATCCAC TGGACCTAGG 
AAAACCGAGG TGTTGAGAGC AGGCCACCAG CGCAATGCCA GCGACCTTCT TGACATAGGA 
TATATGGGCT CCAACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862478.1
CDS	400..5004
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDDLPPPPDPPPGQGLRQQIGPSQQAGNVENSAERKGSSLERQHASSLEDTKSSLDCPARTSLEWQRQTQEWISSTERQEDIRKAPHKQGVGSEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017006989.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006989.1

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCCCCC ACAGTTTGTG 
GTTCGGCCAA GAGATCAGAT TGTTGCTCAA GGTCGAACAG TGACATTTCC CTGTGAAACT 
AAAGGAAACC CACAGCCAGC TGTTTTTTGG CAGAAAGAAG GCAGCCAGAA CCTACTTTTC 
CCAAACCAAC CCCAGCAGCC CAACAGTAGA TGCTCAGTGT CACCAACTGG AGACCTCACA 
ATCACCAACA TTCAACGTTC CGACGCGGGT TACTACATCT GCCAGGCTTT AACTGTGGCA 
GGAAGCATTT TAGCAAAAGC TCAACTGGAG GTTACTGATG TTTTGACAGA TAGACCTCCA 
CCTATAATTC TACAAGGCCC AGCCAACCAA ACGCTGGCAG TGGATGGTAC AGCGTTACTG 
AAATGTAAAG CCACTGGTGA TCCTCTTCCT GTAATTAGCT GGTTAAAGGA GGGATTTACT 
TTTCCGGGTA GAGATCCAAG AGCAACAATT CAAGAGCAAG GCACACTGCA GATTAAGAAT 
TTACGGATTT CTGATACTGG CACTTATACT TGTGTGGCTA CAAGTTCAAG TGGAGAGACT 
TCCTGGAGTG CAGTGCTGGA TGTGACAGAG TCTGGAGCAA CAATCAGTAA AAACTATGAT 
TTAAGTGACC TGCCAGGGCC ACCATCCAAA CCGCAGGTCA CTGATGTTAC TAAGAACAGT 
GTCACCTTGT CCTGGCAGCC AGGTACCCCT GGAACCCTTC CAGCAAGTGC ATATATCATT 
GAGGCTTTCA GCCAATCAGT GAGCAACAGC TGGCAGACCG TGGCAAACCA TGTAAAGACC 
ACCCTCTATA CTGTAAGAGG ACTGCGGCCC AATACAATCT ACTTATTCAT GGTCAGAGCG 
ATCAACCCCC AAGGTCTCAG TGACCCAAGT CCCATGTCAG ATCCTGTGCG CACACAAGAT 
ATCAGCCCAC CAGCACAAGG AGTGGACCAC AGGCAAGTGC AGAAAGAGCT AGGAGATGTC 
CTTGTCCGTC TTCATAATCC AGTTGTGCTG ACTCCCACCA CGGTTCAGGT CACATGGACG 
GTTGATCGCC AACCCCAGTT TATCCAAGGC TACCGAGTGA TGTATCGTCA GACTTCAGGT 
CTGCAGGCGA CATCTTCGTG GCAGAATTTA GATGCCAAAG TCCCGACTGA ACGAAGTGCT 
GTCTTAGTCA ACCTGAAAAA GGGGGTGACT TATGAAATTA AAGTACGGCC ATATTTTAAT 
GAGTTCCAAG GAATGGATAG TGAATCTAAA ACGGTTCGTA CTACTGAAGA AGCCCCAAGT 
GCCCCACCAC AGTCTGTCAC TGTACTGACA GTTGGAAGCT ACAATAGCAC AAGTATTAGT 
GTTTCCTGGG ATCCTCCTCC TCCAGATCAC CAGAATGGAA TTATCCAAGA ATACAAGATC 
TGGTGTCTAG GAAATGAAAC GCGATTCCAT ATCAACAAAA CTGTGGATGC AGCCATTCGG 
TCCGTAATAA TTGGTGGATT ATTCCCAGGT ATTCAATACC GGGTAGAGGT TGCAGCTAGT 
ACCAGTGCAG GGGTTGGAGT AAAGAGTGAG CCACAGCCAA TAATAATCGG GAGACGCAAT 
GAAGTTGTCA TTACTGAAAA CAATAACAGC ATAACTGAGC AAATCACTGA TGTGGTGAAG 
CAACCAGCCT TTATAGCTGG TATTGGTGGT GCCTGCTGGG TAATTCTGAT GGGTTTTAGC 
ATATGGTTGT ATTGGCGAAG AAAGAAGAGG AAGGGACTCA GTAATTATGC TGTTACGTTT 
CAAAGAGGAG ATGGAGGACT AATGAGCAAT GGAAGCCGTC CAGGTCTTCT CAATGCTGGT 
GATCCCAGCT ATCCATGGCT TGCTGATTCT TGGCCAGCCA CGAGCTTGCC AGTAAATAAT 
AGCAACAGTG GCCCAAATGA GATTGGAAAT TTTGGCCGTG GAGATGTGCT GCCACCAGTT 
CCAGGCCAAG GGGATAAAAC AGCAACGATG CTCTCAGATG GAGCCATTTA TAGTAGCATT 
GACTTCACTA CCAAAACCAG TTACAACAGT TCCAGCCAAA TAACACAGGC TACCCCATAT 
GCCACGACAC AGATCTTGCA TTCCAACAGC ATACATGAAT TGGCTGTCGA TCTGCCTGAT 
CCACAATGGA AAAGCTCAAT TCAGCAAAAA ACAGATCTGA TGGGATTTGG TTATTCTCTA 
CCTGATCAGA ACAAAGGTAA CAATGCCTTA CTTTACATTC CTGACTACCG ATTGGCTGAG 
GGATTGTCTA ATAGAATGCC ACACAACCAG TCTCAGGATT TCAGCACCAC CAGCTCTCAC 
AACAGCTCAG AAAGGAGTGG CAGTCTCTCA GGTGGGAAAG GTGGAAAAAA GAAGAAAAAT 
AAAAACTCTT CTAAACCACA GAAAAACAAT GGATCCACTT GGGCCAATGT CCCTCTACCT 
CCCCCCCCAG TCCAGCCCCT TCCTGGCACG GAGCTGGAAC ACTATGCAGT GGAACAACAA 
GAAAATGGCT ATGACAGTGA TAGCTGGTGC CCACCATTGC CAGTACAAAC TTACTTACAC 
CAAGGTCTGG AAGATGAACT GGAAGAAGAT GATGATAGGG TCCCAACACC TCCTGTTCGA 
GGCGTGGCTT CTTCTCCTGC TATCTCCTTT GGACAGCAGT CCACTGCAAC TCTTACTCCA 
TCCCCACGGG AAGAGATGCA ACCCATGCTG CAGGCTCACC TGGATGAGTT GACAAGAGCC 
TATCAGTTTG ATATAGCAAA ACAAACATGG CACATTCAAA GCAATAATCA ACCTCCACAG 
CCTCCAGTTC CACCGTTAGG TTATGTGTCT GGAGCCTTGA TTTCTGATTT GGAAACGGAT 
GTTGCAGATG ATGATGCCGA CGACGAAGAG GAAGCTTTAG AAATCCCCAG GCCCCTGAGA 
GCACTGGACC AGACTCCTGG ATCCAGCATG GACAATCTAG ACAGCTCTGT GACAGGAAAA 
GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC 
AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG 
ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA TCTTCCACCA 
CCACCAGATC CCCCGCCAGG TCAGGGTTTA AGGCAGCAAA TAGGCCCGAG CCAGCAGGCT 
GGTAACGTGG AAAACTCAGC AGAGAGAAAA GGAAGCTCTC TAGAGAGACA ACATGCATCC 
AGCTTAGAAG ACACAAAGAG CTCATTGGAT TGTCCAGCTA GAACCTCCCT AGAGTGGCAG 
CGACAAACCC AGGAATGGAT AAGCTCCACA GAACGACAAG AAGATATACG GAAAGCCCCA 
CACAAACAAG GTGTCGGATC AGAGGAGGCC TTGGTGCCCT ATAGCAAGCC CAGTTTCCCA 
TCTCCAGGTG GCCACAGCTC ATCAGGAACA GCTTCTTCTA AGGGATCCAC TGGACCTAGG 
AAAACCGAGG TGTTGAGAGC AGGCCACCAG CGCAATGCCA GCGACCTTCT TGACATAGGA 
TATATGGGCT CCAACAGTCA AGGACAGTTT ACAGGTGAAT TATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_017006990.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016862479.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X8, mRNA.

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CloneID	OHu94464
Clone ID Related Accession (Same CDS sequence)	XM_017006990.1
Accession Version	XM_017006990.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4596bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT 
AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA 
GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT 
TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA 
GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC 
TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG 
GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT 
TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG 
GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT 
GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT 
CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT 
GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC 
CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC 
TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC 
CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA 
CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG ATCTTCCACC ACCACCAGAT 
CCCCCGCCAG GTCAGGGTTT AAGGCAGCAA ATAGGCCCGA GCCAGCAGGC TGGTAACGTG 
GAAAACTCAG CAGAGAGAAA AGGAAGCTCT CTAGAGAGAC AACATGCATC CAGCTTAGAA 
GACACAAAGA GCTCATTGGA TTGTCCAGCT AGAACCTCCC TAGAGTGGCA GCGACAAACC 
CAGGAATGGA TAAGCTCCAC AGAACGACAA GAAGATATAC GGAAAGCCCC ACACAAACAA 
GGTGTCGGAT CAGAGGAGGC CTTGGTGCCC TATAGCAAGC CCAGTTTCCC ATCTCCAGGT 
GGCCACAGCT CATCAGGAAC AGCTTCTTCT AAGGGATCCA CTGGACCTAG GAAAACCGAG 
GTGTTGAGAG CAGGCCACCA GCGCAATGCC AGCGACCTTC TTGACATAGG ATATATGGGC 
TCCAACAGTC AAGGACAGTT TACAGGTGAA TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016862479.1
CDS	300..4895
Translation	MARRHERVTRRMWTWAPGLLMMTVVFWGHQGNGQGQGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDDLPPPPDPPPGQGLRQQIGPSQQAGNVENSAERKGSSLERQHASSLEDTKSSLDCPARTSLEWQRQTQEWISSTERQEDIRKAPHKQGVGSEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_017006990.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017006990.1

ORF Insert Sequence:

ATGGCCAGAA GACATGAACG TGTCACTAGA AGGATGTGGA CATGGGCTCC GGGACTGTTG 
ATGATGACTG TGGTGTTTTG GGGTCATCAG GGGAATGGAC AAGGCCAAGG ATCGCGTCTT 
CGCCAGGAGG ACTTTCCCCC GCGGATTGTG GAGCATCCTT CCGATGTCAT CGTCTCTAAG 
GGCGAGCCCA CGACTCTGAA CTGCAAGGCG GAGGGCCGGC CAACGCCCAC CATTGAGTGG 
TACAAAGATG GGGAGCGAGT GGAGACTGAC AAGGACGATC CCCGGTCCCA CAGGATGCTT 
CTGCCCAGCG GATCCTTATT CTTCTTGCGC ATCGTGCACG GGCGCAGGAG TAAACCTGAT 
GAAGGAAGCT ACGTTTGTGT TGCGAGGAAC TATCTTGGTG AAGCAGTGAG TCGAAATGCG 
TCTCTGGAAG TGGCATTGTT ACGAGATGAC TTCCGACAAA ACCCCACAGA TGTTGTAGTG 
GCAGCTGGAG AGCCTGCAAT CCTGGAGTGC CAGCCTCCCC GGGGACACCC AGAACCCACC 
ATCTACTGGA AAAAAGACAA AGTTCGAATT GATGACAAGG AAGAAAGAAT AAGTATCCGT 
GGTGGAAAAC TGATGATCTC CAATACCAGG AAAAGTGATG CAGGGATGTA TACTTGTGTT 
GGTACCAATA TGGTGGGAGA AAGGGACAGT GACCCAGCAG AGCTGACTGT CTTTGAACGA 
CCCACATTTC TCAGGAGGCC AATTAACCAG GTGGTACTGG AGGAAGAAGC TGTAGAATTT 
CGTTGTCAAG TCCAAGGAGA TCCTCAACCA ACTGTGAGGT GGAAAAAGGA TGATGCAGAC 
TTGCCAAGAG GAAGGTATGA CATCAAAGAC GATTACACAC TAAGAATTAA AAAGACCATG 
AGTACAGATG AAGGCACCTA TATGTGTATT GCTGAGAATC GGGTTGGAAA AATGGAAGCC 
TCTGCTACAC TCACCGTCCG AGCTCGCCCT GTTGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGCCTT ACTTTACATT CCTGACTACC GATTGGCTGA GGGATTGTCT 
AATAGAATGC CACACAACCA GTCTCAGGAT TTCAGCACCA CCAGCTCTCA CAACAGCTCA 
GAAAGGAGTG GCAGTCTCTC AGGTGGGAAA GGTGGAAAAA AGAAGAAAAA TAAAAACTCT 
TCTAAACCAC AGAAAAACAA TGGATCCACT TGGGCCAATG TCCCTCTACC TCCCCCCCCA 
GTCCAGCCCC TTCCTGGCAC GGAGCTGGAA CACTATGCAG TGGAACAACA AGAAAATGGC 
TATGACAGTG ATAGCTGGTG CCCACCATTG CCAGTACAAA CTTACTTACA CCAAGGTCTG 
GAAGATGAAC TGGAAGAAGA TGATGATAGG GTCCCAACAC CTCCTGTTCG AGGCGTGGCT 
TCTTCTCCTG CTATCTCCTT TGGACAGCAG TCCACTGCAA CTCTTACTCC ATCCCCACGG 
GAAGAGATGC AACCCATGCT GCAGGCTCAC CTGGATGAGT TGACAAGAGC CTATCAGTTT 
GATATAGCAA AACAAACATG GCACATTCAA AGCAATAATC AACCTCCACA GCCTCCAGTT 
CCACCGTTAG GTTATGTGTC TGGAGCCTTG ATTTCTGATT TGGAAACGGA TGTTGCAGAT 
GATGATGCCG ACGACGAAGA GGAAGCTTTA GAAATCCCCA GGCCCCTGAG AGCACTGGAC 
CAGACTCCTG GATCCAGCAT GGACAATCTA GACAGCTCTG TGACAGGAAA AGCCTTTACC 
TCCTCTCAAA GACCTCGACC TACCAGCCCA TTTTCTACTG ACAGTAACAC CAGTGCAGCC 
CTGAGTCAAA GTCAGAGGCC TCGGCCCACT AAAAAACACA AGGGAGGGCG GATGGACCAA 
CAACCAGCAT TGCCTCATCG AAGGGAAGGA ATGACAGATG ATCTTCCACC ACCACCAGAT 
CCCCCGCCAG GTCAGGGTTT AAGGCAGCAA ATAGGCCCGA GCCAGCAGGC TGGTAACGTG 
GAAAACTCAG CAGAGAGAAA AGGAAGCTCT CTAGAGAGAC AACATGCATC CAGCTTAGAA 
GACACAAAGA GCTCATTGGA TTGTCCAGCT AGAACCTCCC TAGAGTGGCA GCGACAAACC 
CAGGAATGGA TAAGCTCCAC AGAACGACAA GAAGATATAC GGAAAGCCCC ACACAAACAA 
GGTGTCGGAT CAGAGGAGGC CTTGGTGCCC TATAGCAAGC CCAGTTTCCC ATCTCCAGGT 
GGCCACAGCT CATCAGGAAC AGCTTCTTCT AAGGGATCCA CTGGACCTAG GAAAACCGAG 
GTGTTGAGAG CAGGCCACCA GCGCAATGCC AGCGACCTTC TTGACATAGG ATATATGGGC 
TCCAACAGTC AAGGACAGTT TACAGGTGAA TTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( NM_002942.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002933.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 2, mRNA.

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CloneID	OHu11403
Clone ID Related Accession (Same CDS sequence)	NM_002942.4 , NM_002942.5
Accession Version	NM_002942.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform ROBO2b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ533874.1 and AC133040.3. On Jun 23, 2010 this sequence version replaced NM_002942.3. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the coding region compared to variant 4. The encoded protein (isoform ROBO2b; PMID 16829019) is shorter than variant 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ533874.1, BC146772.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA 
CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG 
CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC 
AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT 
GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT 
CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG 
TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT 
GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT 
CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT 
CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT 
CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA 
GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG 
CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC 
ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT 
CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002933.1
CDS	644..4780
Translation	MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	NM_002942.4
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002942.4

ORF Insert Sequence:

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA 
CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG 
CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC 
AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT 
GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT 
CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG 
TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT 
GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT 
CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT 
CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT 
CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA 
GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG 
CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC 
ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT 
CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( NM_002942.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002933.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 2, mRNA.

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CloneID	OHu11403
Clone ID Related Accession (Same CDS sequence)	NM_002942.4 , NM_002942.5
Accession Version	NM_002942.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform ROBO2b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117515.8, DQ533874.1 and AC133040.3. On Jun 2, 2019 this sequence version replaced NM_002942.4. Transcript Variant: This variant (2) lacks two alternate in-frame exons in the coding region compared to variant 4. The encoded protein (isoform ROBO2b; PMID 16829019) is shorter than variant 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ533874.1, BC146772.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2154665, SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA 
CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG 
CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC 
AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT 
GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT 
CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG 
TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT 
GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT 
CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT 
CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT 
CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA 
GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG 
CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC 
ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT 
CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002933.1
CDS	688..4824
Translation	MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	NM_002942.5
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002942.5

ORF Insert Sequence:

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCCCC CACAGTTTGT GGTTCGGCCA 
AGAGATCAGA TTGTTGCTCA AGGTCGAACA GTGACATTTC CCTGTGAAAC TAAAGGAAAC 
CCACAGCCAG CTGTTTTTTG GCAGAAAGAA GGCAGCCAGA ACCTACTTTT CCCAAACCAA 
CCCCAGCAGC CCAACAGTAG ATGCTCAGTG TCACCAACTG GAGACCTCAC AATCACCAAC 
ATTCAACGTT CCGACGCGGG TTACTACATC TGCCAGGCTT TAACTGTGGC AGGAAGCATT 
TTAGCAAAAG CTCAACTGGA GGTTACTGAT GTTTTGACAG ATAGACCTCC ACCTATAATT 
CTACAAGGCC CAGCCAACCA AACGCTGGCA GTGGATGGTA CAGCGTTACT GAAATGTAAA 
GCCACTGGTG ATCCTCTTCC TGTAATTAGC TGGTTAAAGG AGGGATTTAC TTTTCCGGGT 
AGAGATCCAA GAGCAACAAT TCAAGAGCAA GGCACACTGC AGATTAAGAA TTTACGGATT 
TCTGATACTG GCACTTATAC TTGTGTGGCT ACAAGTTCAA GTGGAGAGAC TTCCTGGAGT 
GCAGTGCTGG ATGTGACAGA GTCTGGAGCA ACAATCAGTA AAAACTATGA TTTAAGTGAC 
CTGCCAGGGC CACCATCCAA ACCGCAGGTC ACTGATGTTA CTAAGAACAG TGTCACCTTG 
TCCTGGCAGC CAGGTACCCC TGGAACCCTT CCAGCAAGTG CATATATCAT TGAGGCTTTC 
AGCCAATCAG TGAGCAACAG CTGGCAGACC GTGGCAAACC ATGTAAAGAC CACCCTCTAT 
ACTGTAAGAG GACTGCGGCC CAATACAATC TACTTATTCA TGGTCAGAGC GATCAACCCC 
CAAGGTCTCA GTGACCCAAG TCCCATGTCA GATCCTGTGC GCACACAAGA TATCAGCCCA 
CCAGCACAAG GAGTGGACCA CAGGCAAGTG CAGAAAGAGC TAGGAGATGT CCTTGTCCGT 
CTTCATAATC CAGTTGTGCT GACTCCCACC ACGGTTCAGG TCACATGGAC GGTTGATCGC 
CAACCCCAGT TTATCCAAGG CTACCGAGTG ATGTATCGTC AGACTTCAGG TCTGCAGGCG 
ACATCTTCGT GGCAGAATTT AGATGCCAAA GTCCCGACTG AACGAAGTGC TGTCTTAGTC 
AACCTGAAAA AGGGGGTGAC TTATGAAATT AAAGTACGGC CATATTTTAA TGAGTTCCAA 
GGAATGGATA GTGAATCTAA AACGGTTCGT ACTACTGAAG AAGCCCCAAG TGCCCCACCA 
CAGTCTGTCA CTGTACTGAC AGTTGGAAGC TACAATAGCA CAAGTATTAG TGTTTCCTGG 
GATCCTCCTC CTCCAGATCA CCAGAATGGA ATTATCCAAG AATACAAGAT CTGGTGTCTA 
GGAAATGAAA CGCGATTCCA TATCAACAAA ACTGTGGATG CAGCCATTCG GTCCGTAATA 
ATTGGTGGAT TATTCCCAGG TATTCAATAC CGGGTAGAGG TTGCAGCTAG TACCAGTGCA 
GGGGTTGGAG TAAAGAGTGA GCCACAGCCA ATAATAATCG GGAGACGCAA TGAAGTTGTC 
ATTACTGAAA ACAATAACAG CATAACTGAG CAAATCACTG ATGTGGTGAA GCAACCAGCC 
TTTATAGCTG GTATTGGTGG TGCCTGCTGG GTAATTCTGA TGGGTTTTAG CATATGGTTG 
TATTGGCGAA GAAAGAAGAG GAAGGGACTC AGTAATTATG CTGTTACGTT TCAAAGAGGA 
GATGGAGGAC TAATGAGCAA TGGAAGCCGT CCAGGTCTTC TCAATGCTGG TGATCCCAGC 
TATCCATGGC TTGCTGATTC TTGGCCAGCC ACGAGCTTGC CAGTAAATAA TAGCAACAGT 
GGCCCAAATG AGATTGGAAA TTTTGGCCGT GGAGATGTGC TGCCACCAGT TCCAGGCCAA 
GGGGATAAAA CAGCAACGAT GCTCTCAGAT GGAGCCATTT ATAGTAGCAT TGACTTCACT 
ACCAAAACCA GTTACAACAG TTCCAGCCAA ATAACACAGG CTACCCCATA TGCCACGACA 
CAGATCTTGC ATTCCAACAG CATACATGAA TTGGCTGTCG ATCTGCCTGA TCCACAATGG 
AAAAGCTCAA TTCAGCAAAA AACAGATCTG ATGGGATTTG GTTATTCTCT ACCTGATCAG 
AACAAAGGTA ACAATGGTGG GAAAGGTGGA AAAAAGAAGA AAAATAAAAA CTCTTCTAAA 
CCACAGAAAA ACAATGGATC CACTTGGGCC AATGTCCCTC TACCTCCCCC CCCAGTCCAG 
CCCCTTCCTG GCACGGAGCT GGAACACTAT GCAGTGGAAC AACAAGAAAA TGGCTATGAC 
AGTGATAGCT GGTGCCCACC ATTGCCAGTA CAAACTTACT TACACCAAGG TCTGGAAGAT 
GAACTGGAAG AAGATGATGA TAGGGTCCCA ACACCTCCTG TTCGAGGCGT GGCTTCTTCT 
CCTGCTATCT CCTTTGGACA GCAGTCCACT GCAACTCTTA CTCCATCCCC ACGGGAAGAG 
ATGCAACCCA TGCTGCAGGC TCACCTGGAT GAGTTGACAA GAGCCTATCA GTTTGATATA 
GCAAAACAAA CATGGCACAT TCAAAGCAAT AATCAACCTC CACAGCCTCC AGTTCCACCG 
TTAGGTTATG TGTCTGGAGC CTTGATTTCT GATTTGGAAA CGGATGTTGC AGATGATGAT 
GCCGACGACG AAGAGGAAGC TTTAGAAATC CCCAGGCCCC TGAGAGCACT GGACCAGACT 
CCTGGATCCA GCATGGACAA TCTAGACAGC TCTGTGACAG GAAAAGCCTT TACCTCCTCT 
CAAAGACCTC GACCTACCAG CCCATTTTCT ACTGACAGTA ACACCAGTGC AGCCCTGAGT 
CAAAGTCAGA GGCCTCGGCC CACTAAAAAA CACAAGGGAG GGCGGATGGA CCAACAACCA 
GCATTGCCTC ATCGAAGGGA AGGAATGACA GATGAGGAGG CCTTGGTGCC CTATAGCAAG 
CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC 
ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT 
CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( NM_001290040.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276969.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu12785
Clone ID Related Accession (Same CDS sequence)	NM_001290040.1 , NM_001290040.2
Accession Version	NM_001290040.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-29
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ533874.1, BC144303.1 and AC133040.3. On Feb 22, 2014 this sequence version replaced XM_005264768.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144303.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA 
AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC 
CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA 
AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA 
GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC 
GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT 
CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT 
GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA 
CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGTTCAATG 
GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT 
GTAGGTAGCT CCTCAGATGG CTCTATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG 
GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGAAAA 
GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC 
AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG 
ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA GGAGGCCTTG 
GTGCCCTATA GCAAGCCCAG TTTCCCATCT CCAGGTGGCC ACAGCTCATC AGGAACAGCT 
TCTTCTAAGG GATCCACTGG ACCTAGGAAA ACCGAGGTGT TGAGAGCAGG CCACCAGCGC 
AATGCCAGCG ACCTTCTTGA CATAGGATAT ATGGGCTCCA ACAGTCAAGG ACAGTTTACA 
GGTGAATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276969.1
CDS	644..4975
Translation	MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAQALVAAADKAGFRLDGTSLTRTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	NM_001290040.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290040.1

ORF Insert Sequence:

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA 
AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC 
CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA 
AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA 
GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC 
GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT 
CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT 
GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA 
CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGTTCAATG 
GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT 
GTAGGTAGCT CCTCAGATGG CTCTATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG 
GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGAAAA 
GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC 
AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG 
ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA GGAGGCCTTG 
GTGCCCTATA GCAAGCCCAG TTTCCCATCT CCAGGTGGCC ACAGCTCATC AGGAACAGCT 
TCTTCTAAGG GATCCACTGG ACCTAGGAAA ACCGAGGTGT TGAGAGCAGG CCACCAGCGC 
AATGCCAGCG ACCTTCTTGA CATAGGATAT ATGGGCTCCA ACAGTCAAGG ACAGTTTACA 
GGTGAATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( NM_001290040.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276969.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu12785
Clone ID Related Accession (Same CDS sequence)	NM_001290040.1 , NM_001290040.2
Accession Version	NM_001290040.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4332bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform 4 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117515.8, DQ533874.1, BC144303.1 and AC133040.3. On Jun 2, 2019 this sequence version replaced NM_001290040.1. Transcript Variant: This variant (4) represents the longest transcript and encodes the longest isoform (4). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC144303.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA 
AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC 
CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA 
AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA 
GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC 
GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT 
CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT 
GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA 
CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGTTCAATG 
GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT 
GTAGGTAGCT CCTCAGATGG CTCTATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG 
GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGAAAA 
GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC 
AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG 
ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA GGAGGCCTTG 
GTGCCCTATA GCAAGCCCAG TTTCCCATCT CCAGGTGGCC ACAGCTCATC AGGAACAGCT 
TCTTCTAAGG GATCCACTGG ACCTAGGAAA ACCGAGGTGT TGAGAGCAGG CCACCAGCGC 
AATGCCAGCG ACCTTCTTGA CATAGGATAT ATGGGCTCCA ACAGTCAAGG ACAGTTTACA 
GGTGAATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276969.1
CDS	688..5019
Translation	MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAQALVAAADKAGFRLDGTSLTRTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	NM_001290040.2
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290040.2

ORF Insert Sequence:

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA 
AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC 
CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA 
AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA 
GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC 
GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT 
CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT 
GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA 
CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGTTCAATG 
GTAAATGGAT GGGGCTCTGC ATCTGATGAG GATCGTAACT TTTCTAGTCA TAGATCTAGT 
GTAGGTAGCT CCTCAGATGG CTCTATCTTT GCCAGCGGCA GTTTTGCACA AGCACTGGTG 
GCAGCAGCAG ATAAAGCTGG TTTTAGGCTG GATGGAACCA GCCTTACAAG AACAGGAAAA 
GCCTTTACCT CCTCTCAAAG ACCTCGACCT ACCAGCCCAT TTTCTACTGA CAGTAACACC 
AGTGCAGCCC TGAGTCAAAG TCAGAGGCCT CGGCCCACTA AAAAACACAA GGGAGGGCGG 
ATGGACCAAC AACCAGCATT GCCTCATCGA AGGGAAGGAA TGACAGATGA GGAGGCCTTG 
GTGCCCTATA GCAAGCCCAG TTTCCCATCT CCAGGTGGCC ACAGCTCATC AGGAACAGCT 
TCTTCTAAGG GATCCACTGG ACCTAGGAAA ACCGAGGTGT TGAGAGCAGG CCACCAGCGC 
AATGCCAGCG ACCTTCTTGA CATAGGATAT ATGGGCTCCA ACAGTCAAGG ACAGTTTACA 
GGTGAATTAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( NM_001290039.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276968.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 3, mRNA.

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CloneID	OHu12905
Clone ID Related Accession (Same CDS sequence)	NM_001290039.2 , NM_001290039.1
Accession Version	NM_001290039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC117515.8, DQ533874.1, BC144306.1, AC117516.5 and AC133040.3. On Jun 2, 2019 this sequence version replaced NM_001290039.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region compared to variant 4. The encoded protein (isoform 3) is shorter than variant 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.13775.1, SRR1803613.148636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA 
AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC 
CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA 
AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA 
GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC 
GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT 
CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT 
GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA 
CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGAAAAGCC 
TTTACCTCCT CTCAAAGACC TCGACCTACC AGCCCATTTT CTACTGACAG TAACACCAGT 
GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG AGGGCGGATG 
GACCAACAAC CAGCATTGCC TCATCGAAGG GAAGGAATGA CAGATGAGGA GGCCTTGGTG 
CCCTATAGCA AGCCCAGTTT CCCATCTCCA GGTGGCCACA GCTCATCAGG AACAGCTTCT 
TCTAAGGGAT CCACTGGACC TAGGAAAACC GAGGTGTTGA GAGCAGGCCA CCAGCGCAAT 
GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCCAACA GTCAAGGACA GTTTACAGGT 
GAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276968.1
CDS	688..4836
Translation	MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	NM_001290039.2
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290039.2

ORF Insert Sequence:

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA 
AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC 
CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA 
AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA 
GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC 
GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT 
CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT 
GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA 
CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGAAAAGCC 
TTTACCTCCT CTCAAAGACC TCGACCTACC AGCCCATTTT CTACTGACAG TAACACCAGT 
GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG AGGGCGGATG 
GACCAACAAC CAGCATTGCC TCATCGAAGG GAAGGAATGA CAGATGAGGA GGCCTTGGTG 
CCCTATAGCA AGCCCAGTTT CCCATCTCCA GGTGGCCACA GCTCATCAGG AACAGCTTCT 
TCTAAGGGAT CCACTGGACC TAGGAAAACC GAGGTGTTGA GAGCAGGCCA CCAGCGCAAT 
GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCCAACA GTCAAGGACA GTTTACAGGT 
GAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( NM_001290039.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001276968.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 3, mRNA.

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CloneID	OHu12905
Clone ID Related Accession (Same CDS sequence)	NM_001290039.2 , NM_001290039.1
Accession Version	NM_001290039.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4149bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform 3 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DQ533874.1, BC144306.1, AC117516.5 and AC133040.3. On Feb 22, 2014 this sequence version replaced XM_005264769.1. Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region compared to variant 4. The encoded protein (isoform 3) is shorter than variant 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.13775.1, SRR1803613.148636.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142363, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA 
AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC 
CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA 
AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA 
GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC 
GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT 
CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT 
GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA 
CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGAAAAGCC 
TTTACCTCCT CTCAAAGACC TCGACCTACC AGCCCATTTT CTACTGACAG TAACACCAGT 
GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG AGGGCGGATG 
GACCAACAAC CAGCATTGCC TCATCGAAGG GAAGGAATGA CAGATGAGGA GGCCTTGGTG 
CCCTATAGCA AGCCCAGTTT CCCATCTCCA GGTGGCCACA GCTCATCAGG AACAGCTTCT 
TCTAAGGGAT CCACTGGACC TAGGAAAACC GAGGTGTTGA GAGCAGGCCA CCAGCGCAAT 
GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCCAACA GTCAAGGACA GTTTACAGGT 
GAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001276968.1
CDS	644..4792
Translation	MSLLMFTQLLLCGFLYVRVDGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	NM_001290039.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001290039.1

ORF Insert Sequence:

ATGAGTCTGC TGATGTTTAC ACAACTACTG CTCTGTGGAT TTTTATATGT TCGGGTTGAT 
GGATCGCGTC TTCGCCAGGA GGACTTTCCC CCGCGGATTG TGGAGCATCC TTCCGATGTC 
ATCGTCTCTA AGGGCGAGCC CACGACTCTG AACTGCAAGG CGGAGGGCCG GCCAACGCCC 
ACCATTGAGT GGTACAAAGA TGGGGAGCGA GTGGAGACTG ACAAGGACGA TCCCCGGTCC 
CACAGGATGC TTCTGCCCAG CGGATCCTTA TTCTTCTTGC GCATCGTGCA CGGGCGCAGG 
AGTAAACCTG ATGAAGGAAG CTACGTTTGT GTTGCGAGGA ACTATCTTGG TGAAGCAGTG 
AGTCGAAATG CGTCTCTGGA AGTGGCATTG TTACGAGATG ACTTCCGACA AAACCCCACA 
GATGTTGTAG TGGCAGCTGG AGAGCCTGCA ATCCTGGAGT GCCAGCCTCC CCGGGGACAC 
CCAGAACCCA CCATCTACTG GAAAAAAGAC AAAGTTCGAA TTGATGACAA GGAAGAAAGA 
ATAAGTATCC GTGGTGGAAA ACTGATGATC TCCAATACCA GGAAAAGTGA TGCAGGGATG 
TATACTTGTG TTGGTACCAA TATGGTGGGA GAAAGGGACA GTGACCCAGC AGAGCTGACT 
GTCTTTGAAC GACCCACATT TCTCAGGAGG CCAATTAACC AGGTGGTACT GGAGGAAGAA 
GCTGTAGAAT TTCGTTGTCA AGTCCAAGGA GATCCTCAAC CAACTGTGAG GTGGAAAAAG 
GATGATGCAG ACTTGCCAAG AGGAAGGTAT GACATCAAAG ACGATTACAC ACTAAGAATT 
AAAAAGACCA TGAGTACAGA TGAAGGCACC TATATGTGTA TTGCTGAGAA TCGGGTTGGA 
AAAATGGAAG CCTCTGCTAC ACTCACCGTC CGAGCTCGCC CTGTTGCTCC CCCACAGTTT 
GTGGTTCGGC CAAGAGATCA GATTGTTGCT CAAGGTCGAA CAGTGACATT TCCCTGTGAA 
ACTAAAGGAA ACCCACAGCC AGCTGTTTTT TGGCAGAAAG AAGGCAGCCA GAACCTACTT 
TTCCCAAACC AACCCCAGCA GCCCAACAGT AGATGCTCAG TGTCACCAAC TGGAGACCTC 
ACAATCACCA ACATTCAACG TTCCGACGCG GGTTACTACA TCTGCCAGGC TTTAACTGTG 
GCAGGAAGCA TTTTAGCAAA AGCTCAACTG GAGGTTACTG ATGTTTTGAC AGATAGACCT 
CCACCTATAA TTCTACAAGG CCCAGCCAAC CAAACGCTGG CAGTGGATGG TACAGCGTTA 
CTGAAATGTA AAGCCACTGG TGATCCTCTT CCTGTAATTA GCTGGTTAAA GGAGGGATTT 
ACTTTTCCGG GTAGAGATCC AAGAGCAACA ATTCAAGAGC AAGGCACACT GCAGATTAAG 
AATTTACGGA TTTCTGATAC TGGCACTTAT ACTTGTGTGG CTACAAGTTC AAGTGGAGAG 
ACTTCCTGGA GTGCAGTGCT GGATGTGACA GAGTCTGGAG CAACAATCAG TAAAAACTAT 
GATTTAAGTG ACCTGCCAGG GCCACCATCC AAACCGCAGG TCACTGATGT TACTAAGAAC 
AGTGTCACCT TGTCCTGGCA GCCAGGTACC CCTGGAACCC TTCCAGCAAG TGCATATATC 
ATTGAGGCTT TCAGCCAATC AGTGAGCAAC AGCTGGCAGA CCGTGGCAAA CCATGTAAAG 
ACCACCCTCT ATACTGTAAG AGGACTGCGG CCCAATACAA TCTACTTATT CATGGTCAGA 
GCGATCAACC CCCAAGGTCT CAGTGACCCA AGTCCCATGT CAGATCCTGT GCGCACACAA 
GATATCAGCC CACCAGCACA AGGAGTGGAC CACAGGCAAG TGCAGAAAGA GCTAGGAGAT 
GTCCTTGTCC GTCTTCATAA TCCAGTTGTG CTGACTCCCA CCACGGTTCA GGTCACATGG 
ACGGTTGATC GCCAACCCCA GTTTATCCAA GGCTACCGAG TGATGTATCG TCAGACTTCA 
GGTCTGCAGG CGACATCTTC GTGGCAGAAT TTAGATGCCA AAGTCCCGAC TGAACGAAGT 
GCTGTCTTAG TCAACCTGAA AAAGGGGGTG ACTTATGAAA TTAAAGTACG GCCATATTTT 
AATGAGTTCC AAGGAATGGA TAGTGAATCT AAAACGGTTC GTACTACTGA AGAAGCCCCA 
AGTGCCCCAC CACAGTCTGT CACTGTACTG ACAGTTGGAA GCTACAATAG CACAAGTATT 
AGTGTTTCCT GGGATCCTCC TCCTCCAGAT CACCAGAATG GAATTATCCA AGAATACAAG 
ATCTGGTGTC TAGGAAATGA AACGCGATTC CATATCAACA AAACTGTGGA TGCAGCCATT 
CGGTCCGTAA TAATTGGTGG ATTATTCCCA GGTATTCAAT ACCGGGTAGA GGTTGCAGCT 
AGTACCAGTG CAGGGGTTGG AGTAAAGAGT GAGCCACAGC CAATAATAAT CGGGAGACGC 
AATGAAGTTG TCATTACTGA AAACAATAAC AGCATAACTG AGCAAATCAC TGATGTGGTG 
AAGCAACCAG CCTTTATAGC TGGTATTGGT GGTGCCTGCT GGGTAATTCT GATGGGTTTT 
AGCATATGGT TGTATTGGCG AAGAAAGAAG AGGAAGGGAC TCAGTAATTA TGCTGTTACG 
TTTCAAAGAG GAGATGGAGG ACTAATGAGC AATGGAAGCC GTCCAGGTCT TCTCAATGCT 
GGTGATCCCA GCTATCCATG GCTTGCTGAT TCTTGGCCAG CCACGAGCTT GCCAGTAAAT 
AATAGCAACA GTGGCCCAAA TGAGATTGGA AATTTTGGCC GTGGAGATGT GCTGCCACCA 
GTTCCAGGCC AAGGGGATAA AACAGCAACG ATGCTCTCAG ATGGAGCCAT TTATAGTAGC 
ATTGACTTCA CTACCAAAAC CAGTTACAAC AGTTCCAGCC AAATAACACA GGCTACCCCA 
TATGCCACGA CACAGATCTT GCATTCCAAC AGCATACATG AATTGGCTGT CGATCTGCCT 
GATCCACAAT GGAAAAGCTC AATTCAGCAA AAAACAGATC TGATGGGATT TGGTTATTCT 
CTACCTGATC AGAACAAAGG TAACAATGGT GGGAAAGGTG GAAAAAAGAA GAAAAATAAA 
AACTCTTCTA AACCACAGAA AAACAATGGA TCCACTTGGG CCAATGTCCC TCTACCTCCC 
CCCCCAGTCC AGCCCCTTCC TGGCACGGAG CTGGAACACT ATGCAGTGGA ACAACAAGAA 
AATGGCTATG ACAGTGATAG CTGGTGCCCA CCATTGCCAG TACAAACTTA CTTACACCAA 
GGTCTGGAAG ATGAACTGGA AGAAGATGAT GATAGGGTCC CAACACCTCC TGTTCGAGGC 
GTGGCTTCTT CTCCTGCTAT CTCCTTTGGA CAGCAGTCCA CTGCAACTCT TACTCCATCC 
CCACGGGAAG AGATGCAACC CATGCTGCAG GCTCACCTGG ATGAGTTGAC AAGAGCCTAT 
CAGTTTGATA TAGCAAAACA AACATGGCAC ATTCAAAGCA ATAATCAACC TCCACAGCCT 
CCAGTTCCAC CGTTAGGTTA TGTGTCTGGA GCCTTGATTT CTGATTTGGA AACGGATGTT 
GCAGATGATG ATGCCGACGA CGAAGAGGAA GCTTTAGAAA TCCCCAGGCC CCTGAGAGCA 
CTGGACCAGA CTCCTGGATC CAGCATGGAC AATCTAGACA GCTCTGTGAC AGGAAAAGCC 
TTTACCTCCT CTCAAAGACC TCGACCTACC AGCCCATTTT CTACTGACAG TAACACCAGT 
GCAGCCCTGA GTCAAAGTCA GAGGCCTCGG CCCACTAAAA AACACAAGGG AGGGCGGATG 
GACCAACAAC CAGCATTGCC TCATCGAAGG GAAGGAATGA CAGATGAGGA GGCCTTGGTG 
CCCTATAGCA AGCCCAGTTT CCCATCTCCA GGTGGCCACA GCTCATCAGG AACAGCTTCT 
TCTAAGGGAT CCACTGGACC TAGGAAAACC GAGGTGTTGA GAGCAGGCCA CCAGCGCAAT 
GCCAGCGACC TTCTTGACAT AGGATATATG GGCTCCAACA GTCAAGGACA GTTTACAGGT 
GAATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_011533981.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532283.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu60280
Clone ID Related Accession (Same CDS sequence)	XM_011533981.2
Accession Version	XM_011533981.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011533981.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT 
GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA 
GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA 
ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA 
CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA 
ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG 
TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT 
CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT 
TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC 
AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT 
GTGACAGGTT CAATGGTAAA TGGATGGGGC TCTGCATCTG ATGAGGATCG TAACTTTTCT 
AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGGCTCTA TCTTTGCCAG CGGCAGTTTT 
GCACAAGCAC TGGTGGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTT 
ACAAGAACAG GAAAAGCCTT TACCTCCTCT CAAAGACCTC GACCTACCAG CCCATTTTCT 
ACTGACAGTA ACACCAGTGC AGCCCTGAGT CAAAGTCAGA GGCCTCGGCC CACTAAAAAA 
CACAAGGGAG GGCGGATGGA CCAACAACCA GCATTGCCTC ATCGAAGGGA AGGAATGACA 
GATGATCTTC CACCACCACC AGATCCCCCG CCAGGTCAGG GTTTAAGGCA GCAAATAGGC 
CCGAGCCAGC AGGCTGGTAA CGTGGAAAAC TCAGCAGAGA GAAAAGGAAG CTCTCTAGAG 
AGACAACATG CATCCAGCTT AGAAGACACA AAGAGCTCAT TGGATTGTCC AGCTAGAACC 
TCCCTAGAGT GGCAGCGACA AACCCAGGAA TGGATAAGCT CCACAGAACG ACAAGAAGAT 
ATACGGAAAG CCCCACACAA ACAAGGTGTC GGATCAGAGG AGGCCTTGGT GCCCTATAGC 
AAGCCCAGTT TCCCATCTCC AGGTGGCCAC AGCTCATCAG GAACAGCTTC TTCTAAGGGA 
TCCACTGGAC CTAGGAAAAC CGAGGTGTTG AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC 
CTTCTTGACA TAGGATATAT GGGCTCCAAC AGTCAAGGAC AGTTTACAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532283.1
CDS	400..5193
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAQALVAAADKAGFRLDGTSLTRTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDDLPPPPDPPPGQGLRQQIGPSQQAGNVENSAERKGSSLERQHASSLEDTKSSLDCPARTSLEWQRQTQEWISSTERQEDIRKAPHKQGVGSEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTE

Target ORF information:

RefSeq Version	XM_011533981.2
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533981.2

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT 
GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA 
GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA 
ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA 
CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA 
ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG 
TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT 
CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT 
TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC 
AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT 
GTGACAGGTT CAATGGTAAA TGGATGGGGC TCTGCATCTG ATGAGGATCG TAACTTTTCT 
AGTCATAGAT CTAGTGTAGG TAGCTCCTCA GATGGCTCTA TCTTTGCCAG CGGCAGTTTT 
GCACAAGCAC TGGTGGCAGC AGCAGATAAA GCTGGTTTTA GGCTGGATGG AACCAGCCTT 
ACAAGAACAG GAAAAGCCTT TACCTCCTCT CAAAGACCTC GACCTACCAG CCCATTTTCT 
ACTGACAGTA ACACCAGTGC AGCCCTGAGT CAAAGTCAGA GGCCTCGGCC CACTAAAAAA 
CACAAGGGAG GGCGGATGGA CCAACAACCA GCATTGCCTC ATCGAAGGGA AGGAATGACA 
GATGATCTTC CACCACCACC AGATCCCCCG CCAGGTCAGG GTTTAAGGCA GCAAATAGGC 
CCGAGCCAGC AGGCTGGTAA CGTGGAAAAC TCAGCAGAGA GAAAAGGAAG CTCTCTAGAG 
AGACAACATG CATCCAGCTT AGAAGACACA AAGAGCTCAT TGGATTGTCC AGCTAGAACC 
TCCCTAGAGT GGCAGCGACA AACCCAGGAA TGGATAAGCT CCACAGAACG ACAAGAAGAT 
ATACGGAAAG CCCCACACAA ACAAGGTGTC GGATCAGAGG AGGCCTTGGT GCCCTATAGC 
AAGCCCAGTT TCCCATCTCC AGGTGGCCAC AGCTCATCAG GAACAGCTTC TTCTAAGGGA 
TCCACTGGAC CTAGGAAAAC CGAGGTGTTG AGAGCAGGCC ACCAGCGCAA TGCCAGCGAC 
CTTCTTGACA TAGGATATAT GGGCTCCAAC AGTCAAGGAC AGTTTACAGA GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_011533982.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532284.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OHu60281
Clone ID Related Accession (Same CDS sequence)	XM_011533982.1
Accession Version	XM_011533982.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4674bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG 
AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT 
CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA 
CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC 
TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT 
GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT 
ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA 
AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT 
CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA 
ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC 
CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA 
GGTTCAATGG TAAATGGATG GGGCTCTGCA TCTGATGAGG ATCGTAACTT TTCTAGTCAT 
AGATCTAGTG TAGGTAGCTC CTCAGATGGC TCTATCTTTG CCAGCGGCAG TTTTGCACAA 
GCACTGGTGG CAGCAGCAGA TAAAGCTGGT TTTAGGCTGG ATGGAACCAG CCTTACAAGA 
ACAGGAAAAG CCTTTACCTC CTCTCAAAGA CCTCGACCTA CCAGCCCATT TTCTACTGAC 
AGTAACACCA GTGCAGCCCT GAGTCAAAGT CAGAGGCCTC GGCCCACTAA AAAACACAAG 
GGAGGGCGGA TGGACCAACA ACCAGCATTG CCTCATCGAA GGGAAGGAAT GACAGATGAT 
CTTCCACCAC CACCAGATCC CCCGCCAGGT CAGGGTTTAA GGCAGCAAAT AGGCCCGAGC 
CAGCAGGCTG GTAACGTGGA AAACTCAGCA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA 
CATGCATCCA GCTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAG AACCTCCCTA 
GAGTGGCAGC GACAAACCCA GGAATGGATA AGCTCCACAG AACGACAAGA AGATATACGG 
AAAGCCCCAC ACAAACAAGG TGTCGGATCA GAGGAGGCCT TGGTGCCCTA TAGCAAGCCC 
AGTTTCCCAT CTCCAGGTGG CCACAGCTCA TCAGGAACAG CTTCTTCTAA GGGATCCACT 
GGACCTAGGA AAACCGAGGT GTTGAGAGCA GGCCACCAGC GCAATGCCAG CGACCTTCTT 
GACATAGGAT ATATGGGCTC CAACAGTCAA GGACAGTTTA CAGGTGAATT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532284.1
CDS	400..5073
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGSMVNGWGSASDEDRNFSSHRSSVGSSSDGSIFASGSFAQALVAAADKAGFRLDGTSLTRTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDDLPPPPDPPPGQGLRQQIGPSQQAGNVENSAERKGSSLERQHASSLEDTKSSLDCPARTSLEWQRQTQEWISSTERQEDIRKAPHKQGVGSEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_011533982.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533982.1

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG 
AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT 
CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA 
CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC 
TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT 
GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT 
ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA 
AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT 
CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA 
ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC 
CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA 
GGTTCAATGG TAAATGGATG GGGCTCTGCA TCTGATGAGG ATCGTAACTT TTCTAGTCAT 
AGATCTAGTG TAGGTAGCTC CTCAGATGGC TCTATCTTTG CCAGCGGCAG TTTTGCACAA 
GCACTGGTGG CAGCAGCAGA TAAAGCTGGT TTTAGGCTGG ATGGAACCAG CCTTACAAGA 
ACAGGAAAAG CCTTTACCTC CTCTCAAAGA CCTCGACCTA CCAGCCCATT TTCTACTGAC 
AGTAACACCA GTGCAGCCCT GAGTCAAAGT CAGAGGCCTC GGCCCACTAA AAAACACAAG 
GGAGGGCGGA TGGACCAACA ACCAGCATTG CCTCATCGAA GGGAAGGAAT GACAGATGAT 
CTTCCACCAC CACCAGATCC CCCGCCAGGT CAGGGTTTAA GGCAGCAAAT AGGCCCGAGC 
CAGCAGGCTG GTAACGTGGA AAACTCAGCA GAGAGAAAAG GAAGCTCTCT AGAGAGACAA 
CATGCATCCA GCTTAGAAGA CACAAAGAGC TCATTGGATT GTCCAGCTAG AACCTCCCTA 
GAGTGGCAGC GACAAACCCA GGAATGGATA AGCTCCACAG AACGACAAGA AGATATACGG 
AAAGCCCCAC ACAAACAAGG TGTCGGATCA GAGGAGGCCT TGGTGCCCTA TAGCAAGCCC 
AGTTTCCCAT CTCCAGGTGG CCACAGCTCA TCAGGAACAG CTTCTTCTAA GGGATCCACT 
GGACCTAGGA AAACCGAGGT GTTGAGAGCA GGCCACCAGC GCAATGCCAG CGACCTTCTT 
GACATAGGAT ATATGGGCTC CAACAGTCAA GGACAGTTTA CAGGTGAATT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_011533983.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532285.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X5, mRNA.

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CloneID	OHu60282
Clone ID Related Accession (Same CDS sequence)	XM_011533983.1
Accession Version	XM_011533983.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4617bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT 
GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA 
GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA 
ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA 
CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA 
ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG 
TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT 
CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT 
TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC 
AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT 
GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT 
GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC 
AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT 
GATCTTCCAC CACCACCAGA TCCCCCGCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG 
AGCCAGCAGG CTGGTAACGT GGAAAACTCA GCAGAGAGAA AAGGAAGCTC TCTAGAGAGA 
CAACATGCAT CCAGCTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACCTCC 
CTAGAGTGGC AGCGACAAAC CCAGGAATGG ATAAGCTCCA CAGAACGACA AGAAGATATA 
CGGAAAGCCC CACACAAACA AGGTGTCGGA TCAGAGGAGG CCTTGGTGCC CTATAGCAAG 
CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC 
ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT 
CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532285.1
CDS	400..5016
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDDLPPPPDPPPGQGLRQQIGPSQQAGNVENSAERKGSSLERQHASSLEDTKSSLDCPARTSLEWQRQTQEWISSTERQEDIRKAPHKQGVGSEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_011533983.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533983.1

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT 
GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA 
GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA 
ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA 
CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA 
ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG 
TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT 
CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT 
TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC 
AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT 
GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT 
GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC 
AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT 
GATCTTCCAC CACCACCAGA TCCCCCGCCA GGTCAGGGTT TAAGGCAGCA AATAGGCCCG 
AGCCAGCAGG CTGGTAACGT GGAAAACTCA GCAGAGAGAA AAGGAAGCTC TCTAGAGAGA 
CAACATGCAT CCAGCTTAGA AGACACAAAG AGCTCATTGG ATTGTCCAGC TAGAACCTCC 
CTAGAGTGGC AGCGACAAAC CCAGGAATGG ATAAGCTCCA CAGAACGACA AGAAGATATA 
CGGAAAGCCC CACACAAACA AGGTGTCGGA TCAGAGGAGG CCTTGGTGCC CTATAGCAAG 
CCCAGTTTCC CATCTCCAGG TGGCCACAGC TCATCAGGAA CAGCTTCTTC TAAGGGATCC 
ACTGGACCTA GGAAAACCGA GGTGTTGAGA GCAGGCCACC AGCGCAATGC CAGCGACCTT 
CTTGACATAG GATATATGGG CTCCAACAGT CAAGGACAGT TTACAGGTGA ATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_011533984.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532286.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X12, mRNA.

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CloneID	OHu60283
Clone ID Related Accession (Same CDS sequence)	XM_011533984.1
Accession Version	XM_011533984.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4344bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT 
GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA 
GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA 
ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA 
CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA 
ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG 
TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT 
CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT 
TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC 
AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT 
GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT 
GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC 
AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT 
GAGGAGGCCT TGGTGCCCTA TAGCAAGCCC AGTTTCCCAT CTCCAGGTGG CCACAGCTCA 
TCAGGAACAG CTTCTTCTAA GGGATCCACT GGACCTAGGA AAACCGAGGT GTTGAGAGCA 
GGCCACCAGC GCAATGCCAG CGACCTTCTT GACATAGGAT ATATGGGCTC CAACAGTCAA 
GGACAGTTTA CAGGTGAATT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532286.1
CDS	400..4743
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNALLYIPDYRLAEGLSNRMPHNQSQDFSTTSSHNSSERSGSLSGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_011533984.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533984.1

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGCCT TACTTTACAT TCCTGACTAC 
CGATTGGCTG AGGGATTGTC TAATAGAATG CCACACAACC AGTCTCAGGA TTTCAGCACC 
ACCAGCTCTC ACAACAGCTC AGAAAGGAGT GGCAGTCTCT CAGGTGGGAA AGGTGGAAAA 
AAGAAGAAAA ATAAAAACTC TTCTAAACCA CAGAAAAACA ATGGATCCAC TTGGGCCAAT 
GTCCCTCTAC CTCCCCCCCC AGTCCAGCCC CTTCCTGGCA CGGAGCTGGA ACACTATGCA 
GTGGAACAAC AAGAAAATGG CTATGACAGT GATAGCTGGT GCCCACCATT GCCAGTACAA 
ACTTACTTAC ACCAAGGTCT GGAAGATGAA CTGGAAGAAG ATGATGATAG GGTCCCAACA 
CCTCCTGTTC GAGGCGTGGC TTCTTCTCCT GCTATCTCCT TTGGACAGCA GTCCACTGCA 
ACTCTTACTC CATCCCCACG GGAAGAGATG CAACCCATGC TGCAGGCTCA CCTGGATGAG 
TTGACAAGAG CCTATCAGTT TGATATAGCA AAACAAACAT GGCACATTCA AAGCAATAAT 
CAACCTCCAC AGCCTCCAGT TCCACCGTTA GGTTATGTGT CTGGAGCCTT GATTTCTGAT 
TTGGAAACGG ATGTTGCAGA TGATGATGCC GACGACGAAG AGGAAGCTTT AGAAATCCCC 
AGGCCCCTGA GAGCACTGGA CCAGACTCCT GGATCCAGCA TGGACAATCT AGACAGCTCT 
GTGACAGGAA AAGCCTTTAC CTCCTCTCAA AGACCTCGAC CTACCAGCCC ATTTTCTACT 
GACAGTAACA CCAGTGCAGC CCTGAGTCAA AGTCAGAGGC CTCGGCCCAC TAAAAAACAC 
AAGGGAGGGC GGATGGACCA ACAACCAGCA TTGCCTCATC GAAGGGAAGG AATGACAGAT 
GAGGAGGCCT TGGTGCCCTA TAGCAAGCCC AGTTTCCCAT CTCCAGGTGG CCACAGCTCA 
TCAGGAACAG CTTCTTCTAA GGGATCCACT GGACCTAGGA AAACCGAGGT GTTGAGAGCA 
GGCCACCAGC GCAATGCCAG CGACCTTCTT GACATAGGAT ATATGGGCTC CAACAGTCAA 
GGACAGTTTA CAGGTGAATT ATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ROBO2 ( XM_011533985.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011532287.1 Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X20, mRNA.

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CloneID	OHu60284
Clone ID Related Accession (Same CDS sequence)	XM_011533985.1
Accession Version	XM_011533985.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4218bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	roundabout homolog 2 isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000003.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG 
AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT 
CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA 
CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC 
TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT 
GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT 
ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA 
AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT 
CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA 
ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC 
CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA 
GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT 
AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA 
GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG 
GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA 
ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC 
CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG 
TTTACAGGTG AATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011532287.1
CDS	400..4617
Translation	MLSSEKVEKITRLELLFVLMLFLSGPTLSKLTRTPWKGKPWKGGSRLRQEDFPPRIVEHPSDVIVSKGEPTTLNCKAEGRPTPTIEWYKDGERVETDKDDPRSHRMLLPSGSLFFLRIVHGRRSKPDEGSYVCVARNYLGEAVSRNASLEVALLRDDFRQNPTDVVVAAGEPAILECQPPRGHPEPTIYWKKDKVRIDDKEERISIRGGKLMISNTRKSDAGMYTCVGTNMVGERDSDPAELTVFERPTFLRRPINQVVLEEEAVEFRCQVQGDPQPTVRWKKDDADLPRGRYDIKDDYTLRIKKTMSTDEGTYMCIAENRVGKMEASATLTVRARPVAPPQFVVRPRDQIVAQGRTVTFPCETKGNPQPAVFWQKEGSQNLLFPNQPQQPNSRCSVSPTGDLTITNIQRSDAGYYICQALTVAGSILAKAQLEVTDVLTDRPPPIILQGPANQTLAVDGTALLKCKATGDPLPVISWLKEGFTFPGRDPRATIQEQGTLQIKNLRISDTGTYTCVATSSSGETSWSAVLDVTESGATISKNYDLSDLPGPPSKPQVTDVTKNSVTLSWQPGTPGTLPASAYIIEAFSQSVSNSWQTVANHVKTTLYTVRGLRPNTIYLFMVRAINPQGLSDPSPMSDPVRTQDISPPAQGVDHRQVQKELGDVLVRLHNPVVLTPTTVQVTWTVDRQPQFIQGYRVMYRQTSGLQATSSWQNLDAKVPTERSAVLVNLKKGVTYEIKVRPYFNEFQGMDSESKTVRTTEEAPSAPPQSVTVLTVGSYNSTSISVSWDPPPPDHQNGIIQEYKIWCLGNETRFHINKTVDAAIRSVIIGGLFPGIQYRVEVAASTSAGVGVKSEPQPIIIGRRNEVVITENNNSITEQITDVVKQPAFIAGIGGACWVILMGFSIWLYWRRKKRKGLSNYAVTFQRGDGGLMSNGSRPGLLNAGDPSYPWLADSWPATSLPVNNSNSGPNEIGNFGRGDVLPPVPGQGDKTATMLSDGAIYSSIDFTTKTSYNSSSQITQATPYATTQILHSNSIHELAVDLPDPQWKSSIQQKTDLMGFGYSLPDQNKGNNGGKGGKKKKNKNSSKPQKNNGSTWANVPLPPPPVQPLPGTELEHYAVEQQENGYDSDSWCPPLPVQTYLHQGLEDELEEDDDRVPTPPVRGVASSPAISFGQQSTATLTPSPREEMQPMLQAHLDELTRAYQFDIAKQTWHIQSNNQPPQPPVPPLGYVSGALISDLETDVADDDADDEEEALEIPRPLRALDQTPGSSMDNLDSSVTGKAFTSSQRPRPTSPFSTDSNTSAALSQSQRPRPTKKHKGGRMDQQPALPHRREGMTDEEALVPYSKPSFPSPGGHSSSGTASSKGSTGPRKTEVLRAGHQRNASDLLDIGYMGSNSQGQFTGEL

Target ORF information:

RefSeq Version	XM_011533985.1
Organism	Homo sapiens(human)
Definition	Homo sapiens roundabout guidance receptor 2 (ROBO2), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011533985.1

ORF Insert Sequence:

ATGTTATCAT CAGAGAAAGT GGAGAAAATT ACTAGGCTTG AACTTCTCTT CGTTTTGATG 
CTCTTCCTCT CTGGACCAAC TCTGAGCAAG CTGACCCGGA CCCCCTGGAA AGGAAAACCT 
TGGAAGGGGG GATCGCGTCT TCGCCAGGAG GACTTTCCCC CGCGGATTGT GGAGCATCCT 
TCCGATGTCA TCGTCTCTAA GGGCGAGCCC ACGACTCTGA ACTGCAAGGC GGAGGGCCGG 
CCAACGCCCA CCATTGAGTG GTACAAAGAT GGGGAGCGAG TGGAGACTGA CAAGGACGAT 
CCCCGGTCCC ACAGGATGCT TCTGCCCAGC GGATCCTTAT TCTTCTTGCG CATCGTGCAC 
GGGCGCAGGA GTAAACCTGA TGAAGGAAGC TACGTTTGTG TTGCGAGGAA CTATCTTGGT 
GAAGCAGTGA GTCGAAATGC GTCTCTGGAA GTGGCATTGT TACGAGATGA CTTCCGACAA 
AACCCCACAG ATGTTGTAGT GGCAGCTGGA GAGCCTGCAA TCCTGGAGTG CCAGCCTCCC 
CGGGGACACC CAGAACCCAC CATCTACTGG AAAAAAGACA AAGTTCGAAT TGATGACAAG 
GAAGAAAGAA TAAGTATCCG TGGTGGAAAA CTGATGATCT CCAATACCAG GAAAAGTGAT 
GCAGGGATGT ATACTTGTGT TGGTACCAAT ATGGTGGGAG AAAGGGACAG TGACCCAGCA 
GAGCTGACTG TCTTTGAACG ACCCACATTT CTCAGGAGGC CAATTAACCA GGTGGTACTG 
GAGGAAGAAG CTGTAGAATT TCGTTGTCAA GTCCAAGGAG ATCCTCAACC AACTGTGAGG 
TGGAAAAAGG ATGATGCAGA CTTGCCAAGA GGAAGGTATG ACATCAAAGA CGATTACACA 
CTAAGAATTA AAAAGACCAT GAGTACAGAT GAAGGCACCT ATATGTGTAT TGCTGAGAAT 
CGGGTTGGAA AAATGGAAGC CTCTGCTACA CTCACCGTCC GAGCTCGCCC TGTTGCTCCC 
CCACAGTTTG TGGTTCGGCC AAGAGATCAG ATTGTTGCTC AAGGTCGAAC AGTGACATTT 
CCCTGTGAAA CTAAAGGAAA CCCACAGCCA GCTGTTTTTT GGCAGAAAGA AGGCAGCCAG 
AACCTACTTT TCCCAAACCA ACCCCAGCAG CCCAACAGTA GATGCTCAGT GTCACCAACT 
GGAGACCTCA CAATCACCAA CATTCAACGT TCCGACGCGG GTTACTACAT CTGCCAGGCT 
TTAACTGTGG CAGGAAGCAT TTTAGCAAAA GCTCAACTGG AGGTTACTGA TGTTTTGACA 
GATAGACCTC CACCTATAAT TCTACAAGGC CCAGCCAACC AAACGCTGGC AGTGGATGGT 
ACAGCGTTAC TGAAATGTAA AGCCACTGGT GATCCTCTTC CTGTAATTAG CTGGTTAAAG 
GAGGGATTTA CTTTTCCGGG TAGAGATCCA AGAGCAACAA TTCAAGAGCA AGGCACACTG 
CAGATTAAGA ATTTACGGAT TTCTGATACT GGCACTTATA CTTGTGTGGC TACAAGTTCA 
AGTGGAGAGA CTTCCTGGAG TGCAGTGCTG GATGTGACAG AGTCTGGAGC AACAATCAGT 
AAAAACTATG ATTTAAGTGA CCTGCCAGGG CCACCATCCA AACCGCAGGT CACTGATGTT 
ACTAAGAACA GTGTCACCTT GTCCTGGCAG CCAGGTACCC CTGGAACCCT TCCAGCAAGT 
GCATATATCA TTGAGGCTTT CAGCCAATCA GTGAGCAACA GCTGGCAGAC CGTGGCAAAC 
CATGTAAAGA CCACCCTCTA TACTGTAAGA GGACTGCGGC CCAATACAAT CTACTTATTC 
ATGGTCAGAG CGATCAACCC CCAAGGTCTC AGTGACCCAA GTCCCATGTC AGATCCTGTG 
CGCACACAAG ATATCAGCCC ACCAGCACAA GGAGTGGACC ACAGGCAAGT GCAGAAAGAG 
CTAGGAGATG TCCTTGTCCG TCTTCATAAT CCAGTTGTGC TGACTCCCAC CACGGTTCAG 
GTCACATGGA CGGTTGATCG CCAACCCCAG TTTATCCAAG GCTACCGAGT GATGTATCGT 
CAGACTTCAG GTCTGCAGGC GACATCTTCG TGGCAGAATT TAGATGCCAA AGTCCCGACT 
GAACGAAGTG CTGTCTTAGT CAACCTGAAA AAGGGGGTGA CTTATGAAAT TAAAGTACGG 
CCATATTTTA ATGAGTTCCA AGGAATGGAT AGTGAATCTA AAACGGTTCG TACTACTGAA 
GAAGCCCCAA GTGCCCCACC ACAGTCTGTC ACTGTACTGA CAGTTGGAAG CTACAATAGC 
ACAAGTATTA GTGTTTCCTG GGATCCTCCT CCTCCAGATC ACCAGAATGG AATTATCCAA 
GAATACAAGA TCTGGTGTCT AGGAAATGAA ACGCGATTCC ATATCAACAA AACTGTGGAT 
GCAGCCATTC GGTCCGTAAT AATTGGTGGA TTATTCCCAG GTATTCAATA CCGGGTAGAG 
GTTGCAGCTA GTACCAGTGC AGGGGTTGGA GTAAAGAGTG AGCCACAGCC AATAATAATC 
GGGAGACGCA ATGAAGTTGT CATTACTGAA AACAATAACA GCATAACTGA GCAAATCACT 
GATGTGGTGA AGCAACCAGC CTTTATAGCT GGTATTGGTG GTGCCTGCTG GGTAATTCTG 
ATGGGTTTTA GCATATGGTT GTATTGGCGA AGAAAGAAGA GGAAGGGACT CAGTAATTAT 
GCTGTTACGT TTCAAAGAGG AGATGGAGGA CTAATGAGCA ATGGAAGCCG TCCAGGTCTT 
CTCAATGCTG GTGATCCCAG CTATCCATGG CTTGCTGATT CTTGGCCAGC CACGAGCTTG 
CCAGTAAATA ATAGCAACAG TGGCCCAAAT GAGATTGGAA ATTTTGGCCG TGGAGATGTG 
CTGCCACCAG TTCCAGGCCA AGGGGATAAA ACAGCAACGA TGCTCTCAGA TGGAGCCATT 
TATAGTAGCA TTGACTTCAC TACCAAAACC AGTTACAACA GTTCCAGCCA AATAACACAG 
GCTACCCCAT ATGCCACGAC ACAGATCTTG CATTCCAACA GCATACATGA ATTGGCTGTC 
GATCTGCCTG ATCCACAATG GAAAAGCTCA ATTCAGCAAA AAACAGATCT GATGGGATTT 
GGTTATTCTC TACCTGATCA GAACAAAGGT AACAATGGTG GGAAAGGTGG AAAAAAGAAG 
AAAAATAAAA ACTCTTCTAA ACCACAGAAA AACAATGGAT CCACTTGGGC CAATGTCCCT 
CTACCTCCCC CCCCAGTCCA GCCCCTTCCT GGCACGGAGC TGGAACACTA TGCAGTGGAA 
CAACAAGAAA ATGGCTATGA CAGTGATAGC TGGTGCCCAC CATTGCCAGT ACAAACTTAC 
TTACACCAAG GTCTGGAAGA TGAACTGGAA GAAGATGATG ATAGGGTCCC AACACCTCCT 
GTTCGAGGCG TGGCTTCTTC TCCTGCTATC TCCTTTGGAC AGCAGTCCAC TGCAACTCTT 
ACTCCATCCC CACGGGAAGA GATGCAACCC ATGCTGCAGG CTCACCTGGA TGAGTTGACA 
AGAGCCTATC AGTTTGATAT AGCAAAACAA ACATGGCACA TTCAAAGCAA TAATCAACCT 
CCACAGCCTC CAGTTCCACC GTTAGGTTAT GTGTCTGGAG CCTTGATTTC TGATTTGGAA 
ACGGATGTTG CAGATGATGA TGCCGACGAC GAAGAGGAAG CTTTAGAAAT CCCCAGGCCC 
CTGAGAGCAC TGGACCAGAC TCCTGGATCC AGCATGGACA ATCTAGACAG CTCTGTGACA 
GGAAAAGCCT TTACCTCCTC TCAAAGACCT CGACCTACCA GCCCATTTTC TACTGACAGT 
AACACCAGTG CAGCCCTGAG TCAAAGTCAG AGGCCTCGGC CCACTAAAAA ACACAAGGGA 
GGGCGGATGG ACCAACAACC AGCATTGCCT CATCGAAGGG AAGGAATGAC AGATGAGGAG 
GCCTTGGTGC CCTATAGCAA GCCCAGTTTC CCATCTCCAG GTGGCCACAG CTCATCAGGA 
ACAGCTTCTT CTAAGGGATC CACTGGACCT AGGAAAACCG AGGTGTTGAG AGCAGGCCAC 
CAGCGCAATG CCAGCGACCT TCTTGACATA GGATATATGG GCTCCAACAG TCAAGGACAG 
TTTACAGGTG AATTATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Robo Ig4 Is a Dimerization Domain.
Journal of molecular biology429(23)3606-3616(2017 Nov)
Yom-Tov G,Barak R,Matalon O,Barda-Saad M,Guez-Haddad J,Opatowsky Y

ROBO2 gene variants in children with primary nonsyndromic vesicoureteral reflux with or without renal hypoplasia/dysplasia.
Pediatric research80(1)72-6(2016 07)
Mitsioni AG,Siomou E,Bouba I,Petridi S,Siamopoulou A,Georgiou I

Correlations between gastric cancer family history and ROBO2 and RASSF2A gene methylations.
Journal of cancer research and therapeutics12(2)597-600(2016 Apr-Jun)
Chang ZW,Dong L,Qin YR,Song M,Guo HY,Zhu QL

Human placental multipotent mesenchymal stromal cells modulate placenta angiogenesis through Slit2-Robo signaling.
Cell adhesion & migration10(1-2)66-76(2016 Mar)
Chen CY,Tsai CH,Chen CY,Wu YH,Chen CP

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ROBO2 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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ORF Insert Sequence:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence: