RFC4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RFC4
Entrez Gene ID	5984
Full Name	replication factor C subunit 4
Synonyms	A1,RFC37
General protein information	Preferred Names replication factor C subunit 4 Names replication factor C subunit 4 A1 37 kDa subunit RF-C 37 kDa subunit RFC 37 kDa subunit activator 1 37 kDa subunit activator 1 subunit 4 replication factor C (activator 1) 4, 37kDa replication factor C 37 kDa subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 3 Map Location: 3q27.3
Summary	The elongation of primed DNA templates by DNA polymerase delta and DNA polymerase epsilon requires the accessory proteins proliferating cell nuclear antigen (PCNA) and replication factor C (RFC). RFC, also named activator 1, is a protein complex consisting of five distinct subunits of 140, 40, 38, 37, and 36 kD. This gene encodes the 37 kD subunit. This subunit forms a core complex with the 36 and 40 kDa subunits. The core complex possesses DNA-dependent ATPase activity, which was found to be stimulated by PCNA in an in vitro system. Alternatively spliced transcript variants encoding the same protein have been reported. [provided by RefSeq, Jul 2008].
Disorder MIM:	102577

mRNA and Protein(s)

mRNA	Protein	Name
NM_181573.2	NP_853551.1	replication factor C subunit 4
NM_002916.3	NP_002907.1	replication factor C subunit 4
NM_002916.4	NP_002907.1	replication factor C subunit 4

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	RFC4	XP_003310208.1
	RFC4	XP_002803097.1
Mus musculus (house mouse)	Rfc4	NP_663455.1
Schizosaccharomyces pombe (fission yeast)	rfc2	NP_594540.1
Arabidopsis thaliana (thale cress)	EMB1968	NP_564148.1
Bos taurus (cattle)	RFC4	NP_001039359.1
Rattus norvegicus (Norway rat)	Rfc4	NP_001099339.1
Gallus gallus (chicken)	RFC4	NP_001006550.1
Danio rerio (zebrafish)	rfc4	NP_999902.2
Xenopus tropicalis (tropical clawed frog)	rfc4	NP_001016363.1
Drosophila melanogaster (fruit fly)	CG8142	NP_573245.1
Saccharomyces cerevisiae (baker's yeast)	RFC2	NP_012602.3
Neurospora crassa	NCU02687	XP_965460.1
Oryza sativa (rice)	Os12g0176500	NP_001066296.2
Homo sapiens (human)	RFC4	NP_853551.1
Canis lupus familiaris (dog)	RFC4	XP_535837.1
Kluyveromyces lactis	KLLA0B13992g	XP_452154.1
Magnaporthe oryzae (rice blast fungus)	MGG_04389	XP_003713504.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003093	XP_312782.3
Eremothecium gossypii	AGOS_AFR422W	NP_985969.2

Gene Ontology
Bibliography

Related articles in PubMed

Systematic identification of key genes and pathways in the development of invasive cervical cancer.
Niu G, Wang D, Pei Y, Sun L
Gene61828-41(2017 Jun)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Levels of human replication factor C4, a clamp loader, correlate with tumor progression and predict the prognosis for colorectal cancer.
Xiang J, Fang L, Luo Y, Yang Z, Liao Y, Cui J, Huang M, Yang Z, Huang Y, Fan X, Wang H, Wang L, Peng J, Wang J
Journal of translational medicine12320(2014 Nov)

ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin.
Lee KY, Fu H, Aladjem MI, Myung K
The Journal of cell biology200(1)31-44(2013 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Expression level of RFC4 was up regulated in cervical squamous cell carcinoma.
Title: Systematic identification of key genes and pathways in the development of invasive cervical cancer.

RFC4 is frequently overexpressed in CRC, and is associated with tumor progression and worse survival outcome. This might be attributed to the regulation of CRC cell proliferation and cell cycle arrest by RFC4
Title: Levels of human replication factor C4, a clamp loader, correlate with tumor progression and predict the prognosis for colorectal cancer.

Observational study of gene-disease association. (HuGE Navigator)
Title: Association between genetic variants in the base excision repair pathway and outcomes after hematopoietic cell transplantations.

Observational study of gene-disease association. (HuGE Navigator)
Title: Polymorphisms in the base excision repair pathway and graft-versus-host disease.

Observational study of gene-disease association. (HuGE Navigator)
Title: Variation within DNA repair pathway genes and risk of multiple sclerosis.

The following RFC4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RFC4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu10762	NM_181573.2 Latest version!	Homo sapiens replication factor C subunit 4 (RFC4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
OHu10762	NM_002916.4 Latest version!	Homo sapiens replication factor C subunit 4 (RFC4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu10762	NM_002916.3	Homo sapiens replication factor C subunit 4 (RFC4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RFC4 ( NM_181573.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_853551.1 Homo sapiens replication factor C subunit 4 (RFC4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10762
Clone ID Related Accession (Same CDS sequence)	NM_181573.2 , NM_002916.3 , NM_002916.4
Accession Version	NM_181573.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	replication factor C subunit 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CX788139.1, AC112907.7 and AA521171.1. On Aug 14, 2008 this sequence version replaced NM_181573.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.563759.1, SRR1163655.193016.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCAAGCAT TTCTTAAAGG TACATCCATC AGTACTAAAC CCCCGCTGAC CAAGGATCGA 
GGAGTAGCTG CCAGTGCGGG AAGTAGCGGA GAGAACAAGA AAGCCAAACC CGTTCCCTGG 
GTGGAAAAAT ATCGCCCAAA ATGTGTGGAT GAAGTTGCTT TCCAGGAAGA AGTGGTTGCA 
GTGCTGAAAA AATCTTTAGA AGGAGCAGAT CTTCCTAATC TCTTGTTTTA CGGACCACCT 
GGAACTGGAA AAACATCCAC TATTTTGGCA GCAGCTAGAG AACTCTTTGG GCCTGAACTT 
TTCCGATTAA GAGTTCTTGA GTTAAATGCA TCTGATGAAC GTGGAATACA AGTAGTTCGA 
GAGAAAGTGA AAAATTTTGC TCAATTAACT GTGTCAGGAA GTCGCTCAGA TGGGAAGCCG 
TGTCCGCCTT TTAAGATTGT GATTCTGGAT GAAGCAGATT CTATGACCTC AGCTGCTCAG 
GCAGCTTTAA GACGTACCAT GGAGAAGGAG TCGAAAACCA CCCGATTCTG TCTTATCTGT 
AACTATGTCA GTCGAATAAT TGAACCCCTG ACCTCTAGAT GTTCAAAATT CCGCTTCAAG 
CCTCTGTCAG ATAAAATTCA ACAGCAGCGA TTACTAGACA TTGCCAAGAA GGAAAATGTC 
AAAATTAGTG ATGAGGGAAT AGCTTATCTT GTTAAAGTGT CAGAAGGAGA CTTAAGAAAA 
GCCATTACAT TTCTTCAAAG CGCTACTCGA TTAACAGGTG GAAAGGAGAT CACAGAGAAA 
GTGATTACAG ACATTGCCGG GGTAATACCA GCTGAGAAAA TTGATGGAGT ATTTGCTGCC 
TGTCAGAGTG GCTCTTTTGA CAAACTAGAA GCTGTGGTCA AGGATTTAAT AGATGAGGGT 
CATGCAGCAA CTCAGCTCGT CAATCAACTC CATGATGTGG TTGTAGAAAA TAACTTATCT 
GATAAACAGA AGTCTATTAT CACAGAAAAA CTTGCCGAAG TTGACAAATG CCTAGCAGAT 
GGTGCTGATG AACATTTGCA ACTCATCAGC CTTTGTGCAA CTGTGATGCA GCAGTTATCT 
CAGAATTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_853551.1
CDS	283..1374
Translation	MQAFLKGTSISTKPPLTKDRGVAASAGSSGENKKAKPVPWVEKYRPKCVDEVAFQEEVVAVLKKSLEGADLPNLLFYGPPGTGKTSTILAAARELFGPELFRLRVLELNASDERGIQVVREKVKNFAQLTVSGSRSDGKPCPPFKIVILDEADSMTSAAQAALRRTMEKESKTTRFCLICNYVSRIIEPLTSRCSKFRFKPLSDKIQQQRLLDIAKKENVKISDEGIAYLVKVSEGDLRKAITFLQSATRLTGGKEITEKVITDIAGVIPAEKIDGVFAACQSGSFDKLEAVVKDLIDEGHAATQLVNQLHDVVVENNLSDKQKSIITEKLAEVDKCLADGADEHLQLISLCATVMQQLSQNC

Target ORF information:

RefSeq Version	NM_181573.2
Organism	Homo sapiens(human)
Definition	Homo sapiens replication factor C subunit 4 (RFC4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_181573.2

ORF Insert Sequence:

ATGCAAGCAT TTCTTAAAGG TACATCCATC AGTACTAAAC CCCCGCTGAC CAAGGATCGA 
GGAGTAGCTG CCAGTGCGGG AAGTAGCGGA GAGAACAAGA AAGCCAAACC CGTTCCCTGG 
GTGGAAAAAT ATCGCCCAAA ATGTGTGGAT GAAGTTGCTT TCCAGGAAGA AGTGGTTGCA 
GTGCTGAAAA AATCTTTAGA AGGAGCAGAT CTTCCTAATC TCTTGTTTTA CGGACCACCT 
GGAACTGGAA AAACATCCAC TATTTTGGCA GCAGCTAGAG AACTCTTTGG GCCTGAACTT 
TTCCGATTAA GAGTTCTTGA GTTAAATGCA TCTGATGAAC GTGGAATACA AGTAGTTCGA 
GAGAAAGTGA AAAATTTTGC TCAATTAACT GTGTCAGGAA GTCGCTCAGA TGGGAAGCCG 
TGTCCGCCTT TTAAGATTGT GATTCTGGAT GAAGCAGATT CTATGACCTC AGCTGCTCAG 
GCAGCTTTAA GACGTACCAT GGAGAAGGAG TCGAAAACCA CCCGATTCTG TCTTATCTGT 
AACTATGTCA GTCGAATAAT TGAACCCCTG ACCTCTAGAT GTTCAAAATT CCGCTTCAAG 
CCTCTGTCAG ATAAAATTCA ACAGCAGCGA TTACTAGACA TTGCCAAGAA GGAAAATGTC 
AAAATTAGTG ATGAGGGAAT AGCTTATCTT GTTAAAGTGT CAGAAGGAGA CTTAAGAAAA 
GCCATTACAT TTCTTCAAAG CGCTACTCGA TTAACAGGTG GAAAGGAGAT CACAGAGAAA 
GTGATTACAG ACATTGCCGG GGTAATACCA GCTGAGAAAA TTGATGGAGT ATTTGCTGCC 
TGTCAGAGTG GCTCTTTTGA CAAACTAGAA GCTGTGGTCA AGGATTTAAT AGATGAGGGT 
CATGCAGCAA CTCAGCTCGT CAATCAACTC CATGATGTGG TTGTAGAAAA TAACTTATCT 
GATAAACAGA AGTCTATTAT CACAGAAAAA CTTGCCGAAG TTGACAAATG CCTAGCAGAT 
GGTGCTGATG AACATTTGCA ACTCATCAGC CTTTGTGCAA CTGTGATGCA GCAGTTATCT 
CAGAATTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFC4 ( NM_002916.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002907.1 Homo sapiens replication factor C subunit 4 (RFC4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10762
Clone ID Related Accession (Same CDS sequence)	NM_181573.2 , NM_002916.3 , NM_002916.4
Accession Version	NM_002916.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-15
Organism	Homo sapiens(human)
Product	replication factor C subunit 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA359927.1, BC017452.1, AC112907.7 and AA521171.1. On Jun 18, 2003 this sequence version replaced NM_002916.2. Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC017452.1, SRR1163657.357301.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAGCAT TTCTTAAAGG TACATCCATC AGTACTAAAC CCCCGCTGAC CAAGGATCGA 
GGAGTAGCTG CCAGTGCGGG AAGTAGCGGA GAGAACAAGA AAGCCAAACC CGTTCCCTGG 
GTGGAAAAAT ATCGCCCAAA ATGTGTGGAT GAAGTTGCTT TCCAGGAAGA AGTGGTTGCA 
GTGCTGAAAA AATCTTTAGA AGGAGCAGAT CTTCCTAATC TCTTGTTTTA CGGACCACCT 
GGAACTGGAA AAACATCCAC TATTTTGGCA GCAGCTAGAG AACTCTTTGG GCCTGAACTT 
TTCCGATTAA GAGTTCTTGA GTTAAATGCA TCTGATGAAC GTGGAATACA AGTAGTTCGA 
GAGAAAGTGA AAAATTTTGC TCAATTAACT GTGTCAGGAA GTCGCTCAGA TGGGAAGCCG 
TGTCCGCCTT TTAAGATTGT GATTCTGGAT GAAGCAGATT CTATGACCTC AGCTGCTCAG 
GCAGCTTTAA GACGTACCAT GGAGAAGGAG TCGAAAACCA CCCGATTCTG TCTTATCTGT 
AACTATGTCA GTCGAATAAT TGAACCCCTG ACCTCTAGAT GTTCAAAATT CCGCTTCAAG 
CCTCTGTCAG ATAAAATTCA ACAGCAGCGA TTACTAGACA TTGCCAAGAA GGAAAATGTC 
AAAATTAGTG ATGAGGGAAT AGCTTATCTT GTTAAAGTGT CAGAAGGAGA CTTAAGAAAA 
GCCATTACAT TTCTTCAAAG CGCTACTCGA TTAACAGGTG GAAAGGAGAT CACAGAGAAA 
GTGATTACAG ACATTGCCGG GGTAATACCA GCTGAGAAAA TTGATGGAGT ATTTGCTGCC 
TGTCAGAGTG GCTCTTTTGA CAAACTAGAA GCTGTGGTCA AGGATTTAAT AGATGAGGGT 
CATGCAGCAA CTCAGCTCGT CAATCAACTC CATGATGTGG TTGTAGAAAA TAACTTATCT 
GATAAACAGA AGTCTATTAT CACAGAAAAA CTTGCCGAAG TTGACAAATG CCTAGCAGAT 
GGTGCTGATG AACATTTGCA ACTCATCAGC CTTTGTGCAA CTGTGATGCA GCAGTTATCT 
CAGAATTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002907.1
CDS	224..1315
Translation	MQAFLKGTSISTKPPLTKDRGVAASAGSSGENKKAKPVPWVEKYRPKCVDEVAFQEEVVAVLKKSLEGADLPNLLFYGPPGTGKTSTILAAARELFGPELFRLRVLELNASDERGIQVVREKVKNFAQLTVSGSRSDGKPCPPFKIVILDEADSMTSAAQAALRRTMEKESKTTRFCLICNYVSRIIEPLTSRCSKFRFKPLSDKIQQQRLLDIAKKENVKISDEGIAYLVKVSEGDLRKAITFLQSATRLTGGKEITEKVITDIAGVIPAEKIDGVFAACQSGSFDKLEAVVKDLIDEGHAATQLVNQLHDVVVENNLSDKQKSIITEKLAEVDKCLADGADEHLQLISLCATVMQQLSQNC

Target ORF information:

RefSeq Version	NM_002916.3
Organism	Homo sapiens(human)
Definition	Homo sapiens replication factor C subunit 4 (RFC4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002916.3

ORF Insert Sequence:

ATGCAAGCAT TTCTTAAAGG TACATCCATC AGTACTAAAC CCCCGCTGAC CAAGGATCGA 
GGAGTAGCTG CCAGTGCGGG AAGTAGCGGA GAGAACAAGA AAGCCAAACC CGTTCCCTGG 
GTGGAAAAAT ATCGCCCAAA ATGTGTGGAT GAAGTTGCTT TCCAGGAAGA AGTGGTTGCA 
GTGCTGAAAA AATCTTTAGA AGGAGCAGAT CTTCCTAATC TCTTGTTTTA CGGACCACCT 
GGAACTGGAA AAACATCCAC TATTTTGGCA GCAGCTAGAG AACTCTTTGG GCCTGAACTT 
TTCCGATTAA GAGTTCTTGA GTTAAATGCA TCTGATGAAC GTGGAATACA AGTAGTTCGA 
GAGAAAGTGA AAAATTTTGC TCAATTAACT GTGTCAGGAA GTCGCTCAGA TGGGAAGCCG 
TGTCCGCCTT TTAAGATTGT GATTCTGGAT GAAGCAGATT CTATGACCTC AGCTGCTCAG 
GCAGCTTTAA GACGTACCAT GGAGAAGGAG TCGAAAACCA CCCGATTCTG TCTTATCTGT 
AACTATGTCA GTCGAATAAT TGAACCCCTG ACCTCTAGAT GTTCAAAATT CCGCTTCAAG 
CCTCTGTCAG ATAAAATTCA ACAGCAGCGA TTACTAGACA TTGCCAAGAA GGAAAATGTC 
AAAATTAGTG ATGAGGGAAT AGCTTATCTT GTTAAAGTGT CAGAAGGAGA CTTAAGAAAA 
GCCATTACAT TTCTTCAAAG CGCTACTCGA TTAACAGGTG GAAAGGAGAT CACAGAGAAA 
GTGATTACAG ACATTGCCGG GGTAATACCA GCTGAGAAAA TTGATGGAGT ATTTGCTGCC 
TGTCAGAGTG GCTCTTTTGA CAAACTAGAA GCTGTGGTCA AGGATTTAAT AGATGAGGGT 
CATGCAGCAA CTCAGCTCGT CAATCAACTC CATGATGTGG TTGTAGAAAA TAACTTATCT 
GATAAACAGA AGTCTATTAT CACAGAAAAA CTTGCCGAAG TTGACAAATG CCTAGCAGAT 
GGTGCTGATG AACATTTGCA ACTCATCAGC CTTTGTGCAA CTGTGATGCA GCAGTTATCT 
CAGAATTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RFC4 ( NM_002916.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002907.1 Homo sapiens replication factor C subunit 4 (RFC4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu10762
Clone ID Related Accession (Same CDS sequence)	NM_181573.2 , NM_002916.3 , NM_002916.4
Accession Version	NM_002916.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	replication factor C subunit 4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA359927.1, BC017452.1, AC112907.7 and AA521171.1. On Jun 2, 2019 this sequence version replaced NM_002916.3. Transcript Variant: This variant (1) differs in the 5' UTR compared to variant 2. Variants 1 and 2 encode the same isoform. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.69115.1, SRR5189667.120619.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCAAGCAT TTCTTAAAGG TACATCCATC AGTACTAAAC CCCCGCTGAC CAAGGATCGA 
GGAGTAGCTG CCAGTGCGGG AAGTAGCGGA GAGAACAAGA AAGCCAAACC CGTTCCCTGG 
GTGGAAAAAT ATCGCCCAAA ATGTGTGGAT GAAGTTGCTT TCCAGGAAGA AGTGGTTGCA 
GTGCTGAAAA AATCTTTAGA AGGAGCAGAT CTTCCTAATC TCTTGTTTTA CGGACCACCT 
GGAACTGGAA AAACATCCAC TATTTTGGCA GCAGCTAGAG AACTCTTTGG GCCTGAACTT 
TTCCGATTAA GAGTTCTTGA GTTAAATGCA TCTGATGAAC GTGGAATACA AGTAGTTCGA 
GAGAAAGTGA AAAATTTTGC TCAATTAACT GTGTCAGGAA GTCGCTCAGA TGGGAAGCCG 
TGTCCGCCTT TTAAGATTGT GATTCTGGAT GAAGCAGATT CTATGACCTC AGCTGCTCAG 
GCAGCTTTAA GACGTACCAT GGAGAAGGAG TCGAAAACCA CCCGATTCTG TCTTATCTGT 
AACTATGTCA GTCGAATAAT TGAACCCCTG ACCTCTAGAT GTTCAAAATT CCGCTTCAAG 
CCTCTGTCAG ATAAAATTCA ACAGCAGCGA TTACTAGACA TTGCCAAGAA GGAAAATGTC 
AAAATTAGTG ATGAGGGAAT AGCTTATCTT GTTAAAGTGT CAGAAGGAGA CTTAAGAAAA 
GCCATTACAT TTCTTCAAAG CGCTACTCGA TTAACAGGTG GAAAGGAGAT CACAGAGAAA 
GTGATTACAG ACATTGCCGG GGTAATACCA GCTGAGAAAA TTGATGGAGT ATTTGCTGCC 
TGTCAGAGTG GCTCTTTTGA CAAACTAGAA GCTGTGGTCA AGGATTTAAT AGATGAGGGT 
CATGCAGCAA CTCAGCTCGT CAATCAACTC CATGATGTGG TTGTAGAAAA TAACTTATCT 
GATAAACAGA AGTCTATTAT CACAGAAAAA CTTGCCGAAG TTGACAAATG CCTAGCAGAT 
GGTGCTGATG AACATTTGCA ACTCATCAGC CTTTGTGCAA CTGTGATGCA GCAGTTATCT 
CAGAATTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002907.1
CDS	205..1296
Translation	MQAFLKGTSISTKPPLTKDRGVAASAGSSGENKKAKPVPWVEKYRPKCVDEVAFQEEVVAVLKKSLEGADLPNLLFYGPPGTGKTSTILAAARELFGPELFRLRVLELNASDERGIQVVREKVKNFAQLTVSGSRSDGKPCPPFKIVILDEADSMTSAAQAALRRTMEKESKTTRFCLICNYVSRIIEPLTSRCSKFRFKPLSDKIQQQRLLDIAKKENVKISDEGIAYLVKVSEGDLRKAITFLQSATRLTGGKEITEKVITDIAGVIPAEKIDGVFAACQSGSFDKLEAVVKDLIDEGHAATQLVNQLHDVVVENNLSDKQKSIITEKLAEVDKCLADGADEHLQLISLCATVMQQLSQNC

Target ORF information:

RefSeq Version	NM_002916.4
Organism	Homo sapiens(human)
Definition	Homo sapiens replication factor C subunit 4 (RFC4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002916.4

ORF Insert Sequence:

ATGCAAGCAT TTCTTAAAGG TACATCCATC AGTACTAAAC CCCCGCTGAC CAAGGATCGA 
GGAGTAGCTG CCAGTGCGGG AAGTAGCGGA GAGAACAAGA AAGCCAAACC CGTTCCCTGG 
GTGGAAAAAT ATCGCCCAAA ATGTGTGGAT GAAGTTGCTT TCCAGGAAGA AGTGGTTGCA 
GTGCTGAAAA AATCTTTAGA AGGAGCAGAT CTTCCTAATC TCTTGTTTTA CGGACCACCT 
GGAACTGGAA AAACATCCAC TATTTTGGCA GCAGCTAGAG AACTCTTTGG GCCTGAACTT 
TTCCGATTAA GAGTTCTTGA GTTAAATGCA TCTGATGAAC GTGGAATACA AGTAGTTCGA 
GAGAAAGTGA AAAATTTTGC TCAATTAACT GTGTCAGGAA GTCGCTCAGA TGGGAAGCCG 
TGTCCGCCTT TTAAGATTGT GATTCTGGAT GAAGCAGATT CTATGACCTC AGCTGCTCAG 
GCAGCTTTAA GACGTACCAT GGAGAAGGAG TCGAAAACCA CCCGATTCTG TCTTATCTGT 
AACTATGTCA GTCGAATAAT TGAACCCCTG ACCTCTAGAT GTTCAAAATT CCGCTTCAAG 
CCTCTGTCAG ATAAAATTCA ACAGCAGCGA TTACTAGACA TTGCCAAGAA GGAAAATGTC 
AAAATTAGTG ATGAGGGAAT AGCTTATCTT GTTAAAGTGT CAGAAGGAGA CTTAAGAAAA 
GCCATTACAT TTCTTCAAAG CGCTACTCGA TTAACAGGTG GAAAGGAGAT CACAGAGAAA 
GTGATTACAG ACATTGCCGG GGTAATACCA GCTGAGAAAA TTGATGGAGT ATTTGCTGCC 
TGTCAGAGTG GCTCTTTTGA CAAACTAGAA GCTGTGGTCA AGGATTTAAT AGATGAGGGT 
CATGCAGCAA CTCAGCTCGT CAATCAACTC CATGATGTGG TTGTAGAAAA TAACTTATCT 
GATAAACAGA AGTCTATTAT CACAGAAAAA CTTGCCGAAG TTGACAAATG CCTAGCAGAT 
GGTGCTGATG AACATTTGCA ACTCATCAGC CTTTGTGCAA CTGTGATGCA GCAGTTATCT 
CAGAATTGTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Systematic identification of key genes and pathways in the development of invasive cervical cancer.
Gene61828-41(2017 Jun)
Niu G,Wang D,Pei Y,Sun L

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Levels of human replication factor C4, a clamp loader, correlate with tumor progression and predict the prognosis for colorectal cancer.
Journal of translational medicine12320(2014 Nov)
Xiang J,Fang L,Luo Y,Yang Z,Liao Y,Cui J,Huang M,Yang Z,Huang Y,Fan X,Wang H,Wang L,Peng J,Wang J

ATAD5 regulates the lifespan of DNA replication factories by modulating PCNA level on the chromatin.
The Journal of cell biology200(1)31-44(2013 Jan)
Lee KY,Fu H,Aladjem MI,Myung K

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RFC4 cDNA ORF clone, Homo sapiens(human)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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