RAG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RAG1
Entrez Gene ID	5896
Full Name	recombination activating 1
Synonyms	RAG-1,RNF74
General protein information	Preferred Names recombination activating 1 Names V(D)J recombination-activating protein 1 RING finger protein 74 recombination activating gene 1 recombination activating protein 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11p12
Summary	The protein encoded by this gene is involved in activation of immunoglobulin V-D-J recombination. The encoded protein is involved in recognition of the DNA substrate, but stable binding and cleavage activity also requires RAG2. Defects in this gene can be the cause of several diseases. [provided by RefSeq, Jul 2008].
Disorder MIM:	179615
Disorder Html:	Severe combined immunodeficiency, B cell-negative, 601457 (3); Omenn syndrome, 603554 (3); Alpha/beta T-cell lymphopenia with gamma/delta T-cell expansion, severe cytomegalovirus infection, and autoimmunity, 609889 (3); Combined cellular and humoral immune defects with granulomas, 233650 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001377280.1	NP_001364209.1	V(D)J recombination-activating protein 1
NM_000448.3	NP_000439.2	V(D)J recombination-activating protein 1
NM_001377277.1	NP_001364206.1	V(D)J recombination-activating protein 1
XM_005253041.4	XP_005253098.1	V(D)J recombination-activating protein 1 isoform X1
NM_001377279.1	NP_001364208.1	V(D)J recombination-activating protein 1
XM_011520250.2	XP_011518552.1	V(D)J recombination-activating protein 1 isoform X1
NM_000448.2	NP_000439.1	V(D)J recombination-activating protein 1
NM_001377278.1	NP_001364207.1	V(D)J recombination-activating protein 1

Pathways from BioSystems

Homologies

Bos taurus (cattle)	RAG1	XP_005216456.1
Rattus norvegicus (Norway rat)	Rag1	NP_445920.1
Canis lupus familiaris (dog)	RAG1	XP_540538.1
Mus musculus (house mouse)	Rag1	NP_033045.2
Gallus gallus (chicken)	RAG1	NP_001026359.1
Xenopus tropicalis (tropical clawed frog)	rag1	XP_002937338.1
Pan troglodytes (chimpanzee)	RAG1	XP_001154240.1
	RAG1	XP_001084631.1
Homo sapiens (human)	RAG1	NP_000439.1
Danio rerio (zebrafish)	rag1	NP_571464.1

Gene Ontology
Bibliography

Related articles in PubMed

Novel RAG1 mutation in a case of severe combined immunodeficiency.
Zhang J, Quintal L, Atkinson A, Williams B, Grunebaum E, Roifman CM
Pediatrics116(3)e445-9(2005 Sep)

The immunophenotypic and immunogenotypic B-cell differentiation arrest in bone marrow of RAG-deficient SCID patients corresponds to residual recombination activities of mutated RAG proteins.
Noordzij JG, de Bruin-Versteeg S, Verkaik NS, Vossen JM, de Groot R, Bernatowska E, Langerak AW, van Gent DC, van Dongen JJ
Blood100(6)2145-52(2002 Sep)

Identical mutations in RAG1 or RAG2 genes leading to defective V(D)J recombinase activity can cause either T-B-severe combined immune deficiency or Omenn syndrome.
Corneo B, Moshous D, G?ng?r T, Wulffraat N, Philippet P, Le Deist FL, Fischer A, de Villartay JP
Blood97(9)2772-6(2001 May)

V(D)J recombination defects in lymphocytes due to RAG mutations: severe immunodeficiency with a spectrum of clinical presentations.
Villa A, Sobacchi C, Notarangelo LD, Bozzi F, Abinun M, Abrahamsen TG, Arkwright PD, Baniyash M, Brooks EG, Conley ME, Cortes P, Duse M, Fasth A, Filipovich AM, Infante AJ, Jones A, Mazzolari E, Muller SM, Pasic S, Rechavi G, Sacco MG, Santagata S, Schroeder ML, Seger R, Strina D, Ugazio A, V?liaho J, Vihinen M, Vogler LB, Ochs H, Vezzoni P, Friedrich W, Schwarz K
Blood97(1)81-8(2001 Jan)

N-terminal RAG1 frameshift mutations in Omenn's syndrome: internal methionine usage leads to partial V(D)J recombination activity and reveals a fundamental role in vivo for the N-terminal domains.
Santagata S, Gomez CA, Sobacchi C, Bozzi F, Abinun M, Pasic S, Cortes P, Vezzoni P, Villa A
Proceedings of the National Academy of Sciences of the United States of America97(26)14572-7(2000 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Our findings suggest that mutations in RAG1, specifically the p.R474C variant, can be associated with relatively mild clinical symptoms or delayed occurrence of T cell and B cell deficiencies but may predispose to progressive multifocal leukoencephalopathy.
Title: Evaluation of RAG1 mutations in an adult with combined immunodeficiency and progressive multifocal leukoencephalopathy.

We report two siblings with SCID and an atypical phenotype of osteopetrosis (OP). A biallelic microdeletion encompassing the 5' region of TRAF6, RAG1 and RAG2 genes was identified. TRAF6, a tumor necrosis factor receptor-associated family member, plays an important role in T cell signaling and in RANKL-dependent osteoclast differentiation and activation but its role in human OP has not been previously reported
Title: Homozygous deletion of RAG1, RAG2 and 5' region TRAF6 causes severe immune suppression and atypical osteopetrosis.

Agammaglobulinemia associated to nasal polyposis due to a hypomorphic RAG1 mutation
Title: Agammaglobulinemia associated to nasal polyposis due to a hypomorphic RAG1 mutation in a 12 years old boy.

mutation c.256_257delAA in RAG1 gene seems to occur quite frequently in the Polish patients with severe combined immunodeficiency and may result in classical OS as well as in severe combined immunodeficiency without clinical and laboratory features of OS when occurred in homozygous state
Title: Mutation c.256_257delAA in RAG1 Gene in Polish Children with Severe Combined Immunodeficiency: Diversity of Clinical Manifestations.

The show that DNA damage caused by RAG1 activity in pre-B cells was able to downmodulate RAG1 expression and activity, confirming the existence of a negative feedback regulatory mechanism.
Title: The DNA Damage Response Regulates RAG1/2 Expression in Pre-B Cells through ATM-FOXO1 Signaling.

The following RAG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RAG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17854	NM_000448.2 Latest version!	Homo sapiens recombination activating 1 (RAG1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$399.50-$559.30 $799.00
OHu44935	NM_000448.3 Latest version!	Homo sapiens recombination activating 1 (RAG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu44935	NM_001377278.1 Latest version!	Homo sapiens recombination activating 1 (RAG1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu44935	NM_001377280.1 Latest version!	Homo sapiens recombination activating 1 (RAG1), transcript variant 5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu44935	NM_001377277.1 Latest version!	Homo sapiens recombination activating 1 (RAG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu44935	NM_001377279.1 Latest version!	Homo sapiens recombination activating 1 (RAG1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
OHu44935	XM_011520250.2 Latest version!	Homo sapiens recombination activating 1 (RAG1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00
OHu44935	XM_005253041.4 Latest version!	Homo sapiens recombination activating 1 (RAG1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$559.30 $799.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RAG1 ( NM_000448.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_000439.1 Homo sapiens recombination activating 1 (RAG1), mRNA.

Price & Availability↑

CloneID	OHu17854
Clone ID Related Accession (Same CDS sequence)	NM_000448.2
Accession Version	NM_000448.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	V(D)J recombination-activating protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC139427.3 and M29474.1. This sequence is a reference standard in the RefSeqGene project. On Apr 4, 2008 this sequence version replaced NM_000448.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: HY099452.1, M29474.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142853, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCGCAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000439.1
CDS	125..3256
Translation	MAASFPPTLGLSSAPDEIQHPHIKFSEWKFKLFRVRSFEKTPEEAQKEKKDSFEGKPSLEQSPAVLDKADGQKPVPTQPLLKAHPKFSKKFHDNEKARGKAIHQANLRHLCRICGNSFRADEHNRRYPVHGPVDGKTLGLLRKKEKRATSWPDLIAKVFRIDVKADVDSIHPTEFCHNCWSIMHRKFSSAPCEVYFPRNVTMEWHPHTPSCDICNTARRGLKRKSLQPNLQLSKKLKTVLDQARQARQRKRRAQARISSKDVMKKIANCSKIHLSTKLLAVDFPEHFVKSISCQICEHILADPVETNCKHVFCRVCILRCLKVMGSYCPSCRYPCFPTDLESPVKSFLSVLNSLMVKCPAKECNEEVSLEKYNHHISSHKESKEIFVHINKGGRPRQHLLSLTRRAQKHRLRELKLQVKAFADKEEGGDVKSVCMTLFLLALRARNEHRQADELEAIMQGKGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEKVLLPGYHHFEWQPPLKNVSSSTDVGIIDGLSGLSSSVDDYPVDTIAKRFRYDSALVSALMDMEEDILEGMRSQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPVVPEKAVRFSFTIMKITIAHSSQNVKVFEEAKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSELMLELGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEASQNLVFHSITRSHAENLERYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEVYKNPNASKEERKRWQATLDKHLRKKMNLKPIMRMNGNFARKLMTKETVDAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYSFNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKCYEMEDVLKHHWLYTSKYLQKFMNAHNALKTSGFTMNPQASLGDPLGIEDSLESQDSMEF

Target ORF information:

RefSeq Version	NM_000448.2
Organism	Homo sapiens(human)
Definition	Homo sapiens recombination activating 1 (RAG1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000448.2

ORF Insert Sequence:

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCGCAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAG1 ( NM_001377280.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001364209.1 Homo sapiens recombination activating 1 (RAG1), transcript variant 5, mRNA.

Price & Availability↑

CloneID	OHu44935
Clone ID Related Accession (Same CDS sequence)	NM_001377280.1 , NM_000448.3 , NM_001377277.1 , XM_005253041.4 , NM_001377279.1 , XM_011520250.2 , NM_001377278.1
Accession Version	NM_001377280.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Homo sapiens(human)
Product	V(D)J recombination-activating protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC061999.6 and AC139427.3. On Dec 24, 2019 this sequence version replaced XM_005253041.4. Transcript Variant: Variants 1 through 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189664.163932.1, DRR138524.268455.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001364209.1
CDS	200..3331
Translation	MAASFPPTLGLSSAPDEIQHPHIKFSEWKFKLFRVRSFEKTPEEAQKEKKDSFEGKPSLEQSPAVLDKADGQKPVPTQPLLKAHPKFSKKFHDNEKARGKAIHQANLRHLCRICGNSFRADEHNRRYPVHGPVDGKTLGLLRKKEKRATSWPDLIAKVFRIDVKADVDSIHPTEFCHNCWSIMHRKFSSAPCEVYFPRNVTMEWHPHTPSCDICNTARRGLKRKSLQPNLQLSKKLKTVLDQARQARQHKRRAQARISSKDVMKKIANCSKIHLSTKLLAVDFPEHFVKSISCQICEHILADPVETNCKHVFCRVCILRCLKVMGSYCPSCRYPCFPTDLESPVKSFLSVLNSLMVKCPAKECNEEVSLEKYNHHISSHKESKEIFVHINKGGRPRQHLLSLTRRAQKHRLRELKLQVKAFADKEEGGDVKSVCMTLFLLALRARNEHRQADELEAIMQGKGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEKVLLPGYHHFEWQPPLKNVSSSTDVGIIDGLSGLSSSVDDYPVDTIAKRFRYDSALVSALMDMEEDILEGMRSQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPVVPEKAVRFSFTIMKITIAHSSQNVKVFEEAKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSELMLELGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEASQNLVFHSITRSHAENLERYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEVYKNPNASKEERKRWQATLDKHLRKKMNLKPIMRMNGNFARKLMTKETVDAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYSFNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKCYEMEDVLKHHWLYTSKYLQKFMNAHNALKTSGFTMNPQASLGDPLGIEDSLESQDSMEF

Target ORF information:

RefSeq Version	NM_001377280.1
Organism	Homo sapiens(human)
Definition	Homo sapiens recombination activating 1 (RAG1), transcript variant 5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377280.1

ORF Insert Sequence:

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAG1 ( NM_000448.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_000439.2 Homo sapiens recombination activating 1 (RAG1), transcript variant 1, mRNA.

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CloneID	OHu44935
Clone ID Related Accession (Same CDS sequence)	NM_001377280.1 , NM_000448.3 , NM_001377277.1 , XM_005253041.4 , NM_001377279.1 , XM_011520250.2 , NM_001377278.1
Accession Version	NM_000448.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-02
Organism	Homo sapiens(human)
Product	V(D)J recombination-activating protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC139427.3 and M29474.1. On Dec 24, 2019 this sequence version replaced NM_000448.2. Transcript Variant: Variants 1 through 5 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: M29474.1, HY073023.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_000439.2
CDS	131..3262
Translation	MAASFPPTLGLSSAPDEIQHPHIKFSEWKFKLFRVRSFEKTPEEAQKEKKDSFEGKPSLEQSPAVLDKADGQKPVPTQPLLKAHPKFSKKFHDNEKARGKAIHQANLRHLCRICGNSFRADEHNRRYPVHGPVDGKTLGLLRKKEKRATSWPDLIAKVFRIDVKADVDSIHPTEFCHNCWSIMHRKFSSAPCEVYFPRNVTMEWHPHTPSCDICNTARRGLKRKSLQPNLQLSKKLKTVLDQARQARQHKRRAQARISSKDVMKKIANCSKIHLSTKLLAVDFPEHFVKSISCQICEHILADPVETNCKHVFCRVCILRCLKVMGSYCPSCRYPCFPTDLESPVKSFLSVLNSLMVKCPAKECNEEVSLEKYNHHISSHKESKEIFVHINKGGRPRQHLLSLTRRAQKHRLRELKLQVKAFADKEEGGDVKSVCMTLFLLALRARNEHRQADELEAIMQGKGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEKVLLPGYHHFEWQPPLKNVSSSTDVGIIDGLSGLSSSVDDYPVDTIAKRFRYDSALVSALMDMEEDILEGMRSQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPVVPEKAVRFSFTIMKITIAHSSQNVKVFEEAKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSELMLELGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEASQNLVFHSITRSHAENLERYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEVYKNPNASKEERKRWQATLDKHLRKKMNLKPIMRMNGNFARKLMTKETVDAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYSFNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKCYEMEDVLKHHWLYTSKYLQKFMNAHNALKTSGFTMNPQASLGDPLGIEDSLESQDSMEF

Target ORF information:

RefSeq Version	NM_000448.3
Organism	Homo sapiens(human)
Definition	Homo sapiens recombination activating 1 (RAG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_000448.3

ORF Insert Sequence:

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAG1 ( NM_001377277.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001364206.1 Homo sapiens recombination activating 1 (RAG1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu44935
Clone ID Related Accession (Same CDS sequence)	NM_001377280.1 , NM_000448.3 , NM_001377277.1 , XM_005253041.4 , NM_001377279.1 , XM_011520250.2 , NM_001377278.1
Accession Version	NM_001377277.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Homo sapiens(human)
Product	V(D)J recombination-activating protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC061999.6 and AC139427.3. Transcript Variant: Variants 1 through 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.85279.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001364206.1
CDS	474..3605
Translation	MAASFPPTLGLSSAPDEIQHPHIKFSEWKFKLFRVRSFEKTPEEAQKEKKDSFEGKPSLEQSPAVLDKADGQKPVPTQPLLKAHPKFSKKFHDNEKARGKAIHQANLRHLCRICGNSFRADEHNRRYPVHGPVDGKTLGLLRKKEKRATSWPDLIAKVFRIDVKADVDSIHPTEFCHNCWSIMHRKFSSAPCEVYFPRNVTMEWHPHTPSCDICNTARRGLKRKSLQPNLQLSKKLKTVLDQARQARQHKRRAQARISSKDVMKKIANCSKIHLSTKLLAVDFPEHFVKSISCQICEHILADPVETNCKHVFCRVCILRCLKVMGSYCPSCRYPCFPTDLESPVKSFLSVLNSLMVKCPAKECNEEVSLEKYNHHISSHKESKEIFVHINKGGRPRQHLLSLTRRAQKHRLRELKLQVKAFADKEEGGDVKSVCMTLFLLALRARNEHRQADELEAIMQGKGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEKVLLPGYHHFEWQPPLKNVSSSTDVGIIDGLSGLSSSVDDYPVDTIAKRFRYDSALVSALMDMEEDILEGMRSQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPVVPEKAVRFSFTIMKITIAHSSQNVKVFEEAKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSELMLELGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEASQNLVFHSITRSHAENLERYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEVYKNPNASKEERKRWQATLDKHLRKKMNLKPIMRMNGNFARKLMTKETVDAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYSFNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKCYEMEDVLKHHWLYTSKYLQKFMNAHNALKTSGFTMNPQASLGDPLGIEDSLESQDSMEF

Target ORF information:

RefSeq Version	NM_001377277.1
Organism	Homo sapiens(human)
Definition	Homo sapiens recombination activating 1 (RAG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377277.1

ORF Insert Sequence:

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAG1 ( NM_001377279.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001364208.1 Homo sapiens recombination activating 1 (RAG1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu44935
Clone ID Related Accession (Same CDS sequence)	NM_001377280.1 , NM_000448.3 , NM_001377277.1 , XM_005253041.4 , NM_001377279.1 , XM_011520250.2 , NM_001377278.1
Accession Version	NM_001377279.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Homo sapiens(human)
Product	V(D)J recombination-activating protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC061999.6 and AC139427.3. On Dec 24, 2019 this sequence version replaced XM_011520250.2. Transcript Variant: Variants 1 through 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications.

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001364208.1
CDS	314..3445
Translation	MAASFPPTLGLSSAPDEIQHPHIKFSEWKFKLFRVRSFEKTPEEAQKEKKDSFEGKPSLEQSPAVLDKADGQKPVPTQPLLKAHPKFSKKFHDNEKARGKAIHQANLRHLCRICGNSFRADEHNRRYPVHGPVDGKTLGLLRKKEKRATSWPDLIAKVFRIDVKADVDSIHPTEFCHNCWSIMHRKFSSAPCEVYFPRNVTMEWHPHTPSCDICNTARRGLKRKSLQPNLQLSKKLKTVLDQARQARQHKRRAQARISSKDVMKKIANCSKIHLSTKLLAVDFPEHFVKSISCQICEHILADPVETNCKHVFCRVCILRCLKVMGSYCPSCRYPCFPTDLESPVKSFLSVLNSLMVKCPAKECNEEVSLEKYNHHISSHKESKEIFVHINKGGRPRQHLLSLTRRAQKHRLRELKLQVKAFADKEEGGDVKSVCMTLFLLALRARNEHRQADELEAIMQGKGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEKVLLPGYHHFEWQPPLKNVSSSTDVGIIDGLSGLSSSVDDYPVDTIAKRFRYDSALVSALMDMEEDILEGMRSQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPVVPEKAVRFSFTIMKITIAHSSQNVKVFEEAKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSELMLELGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEASQNLVFHSITRSHAENLERYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEVYKNPNASKEERKRWQATLDKHLRKKMNLKPIMRMNGNFARKLMTKETVDAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYSFNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKCYEMEDVLKHHWLYTSKYLQKFMNAHNALKTSGFTMNPQASLGDPLGIEDSLESQDSMEF

Target ORF information:

RefSeq Version	NM_001377279.1
Organism	Homo sapiens(human)
Definition	Homo sapiens recombination activating 1 (RAG1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377279.1

ORF Insert Sequence:

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAG1 ( NM_001377278.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001364207.1 Homo sapiens recombination activating 1 (RAG1), transcript variant 3, mRNA.

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CloneID	OHu44935
Clone ID Related Accession (Same CDS sequence)	NM_001377280.1 , NM_000448.3 , NM_001377277.1 , XM_005253041.4 , NM_001377279.1 , XM_011520250.2 , NM_001377278.1
Accession Version	NM_001377278.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-23
Organism	Homo sapiens(human)
Product	V(D)J recombination-activating protein 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC061999.6 and AC139427.3. Transcript Variant: Variants 1 through 5 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189661.23161.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001364207.1
CDS	412..3543
Translation	MAASFPPTLGLSSAPDEIQHPHIKFSEWKFKLFRVRSFEKTPEEAQKEKKDSFEGKPSLEQSPAVLDKADGQKPVPTQPLLKAHPKFSKKFHDNEKARGKAIHQANLRHLCRICGNSFRADEHNRRYPVHGPVDGKTLGLLRKKEKRATSWPDLIAKVFRIDVKADVDSIHPTEFCHNCWSIMHRKFSSAPCEVYFPRNVTMEWHPHTPSCDICNTARRGLKRKSLQPNLQLSKKLKTVLDQARQARQHKRRAQARISSKDVMKKIANCSKIHLSTKLLAVDFPEHFVKSISCQICEHILADPVETNCKHVFCRVCILRCLKVMGSYCPSCRYPCFPTDLESPVKSFLSVLNSLMVKCPAKECNEEVSLEKYNHHISSHKESKEIFVHINKGGRPRQHLLSLTRRAQKHRLRELKLQVKAFADKEEGGDVKSVCMTLFLLALRARNEHRQADELEAIMQGKGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEKVLLPGYHHFEWQPPLKNVSSSTDVGIIDGLSGLSSSVDDYPVDTIAKRFRYDSALVSALMDMEEDILEGMRSQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPVVPEKAVRFSFTIMKITIAHSSQNVKVFEEAKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSELMLELGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEASQNLVFHSITRSHAENLERYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEVYKNPNASKEERKRWQATLDKHLRKKMNLKPIMRMNGNFARKLMTKETVDAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYSFNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKCYEMEDVLKHHWLYTSKYLQKFMNAHNALKTSGFTMNPQASLGDPLGIEDSLESQDSMEF

Target ORF information:

RefSeq Version	NM_001377278.1
Organism	Homo sapiens(human)
Definition	Homo sapiens recombination activating 1 (RAG1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001377278.1

ORF Insert Sequence:

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAG1 ( XM_005253041.4 ) cDNA ORF clone, Homo sapiens(human) -> XP_005253098.1 Homo sapiens recombination activating 1 (RAG1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu44935
Clone ID Related Accession (Same CDS sequence)	NM_001377280.1 , NM_000448.3 , NM_001377277.1 , XM_005253041.4 , NM_001377279.1 , XM_011520250.2 , NM_001377278.1
Accession Version	XM_005253041.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	V(D)J recombination-activating protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005253041.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005253098.1
CDS	187..3318
Translation	MAASFPPTLGLSSAPDEIQHPHIKFSEWKFKLFRVRSFEKTPEEAQKEKKDSFEGKPSLEQSPAVLDKADGQKPVPTQPLLKAHPKFSKKFHDNEKARGKAIHQANLRHLCRICGNSFRADEHNRRYPVHGPVDGKTLGLLRKKEKRATSWPDLIAKVFRIDVKADVDSIHPTEFCHNCWSIMHRKFSSAPCEVYFPRNVTMEWHPHTPSCDICNTARRGLKRKSLQPNLQLSKKLKTVLDQARQARQHKRRAQARISSKDVMKKIANCSKIHLSTKLLAVDFPEHFVKSISCQICEHILADPVETNCKHVFCRVCILRCLKVMGSYCPSCRYPCFPTDLESPVKSFLSVLNSLMVKCPAKECNEEVSLEKYNHHISSHKESKEIFVHINKGGRPRQHLLSLTRRAQKHRLRELKLQVKAFADKEEGGDVKSVCMTLFLLALRARNEHRQADELEAIMQGKGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEKVLLPGYHHFEWQPPLKNVSSSTDVGIIDGLSGLSSSVDDYPVDTIAKRFRYDSALVSALMDMEEDILEGMRSQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPVVPEKAVRFSFTIMKITIAHSSQNVKVFEEAKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSELMLELGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEASQNLVFHSITRSHAENLERYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEVYKNPNASKEERKRWQATLDKHLRKKMNLKPIMRMNGNFARKLMTKETVDAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYSFNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKCYEMEDVLKHHWLYTSKYLQKFMNAHNALKTSGFTMNPQASLGDPLGIEDSLESQDSMEF

Target ORF information:

RefSeq Version	XM_005253041.4
Organism	Homo sapiens(human)
Definition	Homo sapiens recombination activating 1 (RAG1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005253041.4

ORF Insert Sequence:

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RAG1 ( XM_011520250.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011518552.1 Homo sapiens recombination activating 1 (RAG1), transcript variant X1, mRNA.

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CloneID	OHu44935
Clone ID Related Accession (Same CDS sequence)	NM_001377280.1 , NM_000448.3 , NM_001377277.1 , XM_005253041.4 , NM_001377279.1 , XM_011520250.2 , NM_001377278.1
Accession Version	XM_011520250.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3132bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-12
Organism	Homo sapiens(human)
Product	V(D)J recombination-activating protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000011.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011520250.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20190607 Annotation Version :: 109.20190607 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA Annotation Freeze :: 20190607 ##Genome-Annotation-Data-END##

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011518552.1
CDS	301..3432
Translation	MAASFPPTLGLSSAPDEIQHPHIKFSEWKFKLFRVRSFEKTPEEAQKEKKDSFEGKPSLEQSPAVLDKADGQKPVPTQPLLKAHPKFSKKFHDNEKARGKAIHQANLRHLCRICGNSFRADEHNRRYPVHGPVDGKTLGLLRKKEKRATSWPDLIAKVFRIDVKADVDSIHPTEFCHNCWSIMHRKFSSAPCEVYFPRNVTMEWHPHTPSCDICNTARRGLKRKSLQPNLQLSKKLKTVLDQARQARQHKRRAQARISSKDVMKKIANCSKIHLSTKLLAVDFPEHFVKSISCQICEHILADPVETNCKHVFCRVCILRCLKVMGSYCPSCRYPCFPTDLESPVKSFLSVLNSLMVKCPAKECNEEVSLEKYNHHISSHKESKEIFVHINKGGRPRQHLLSLTRRAQKHRLRELKLQVKAFADKEEGGDVKSVCMTLFLLALRARNEHRQADELEAIMQGKGSGLQPAVCLAIRVNTFLSCSQYHKMYRTVKAITGRQIFQPLHALRNAEKVLLPGYHHFEWQPPLKNVSSSTDVGIIDGLSGLSSSVDDYPVDTIAKRFRYDSALVSALMDMEEDILEGMRSQDLDDYLNGPFTVVVKESCDGMGDVSEKHGSGPVVPEKAVRFSFTIMKITIAHSSQNVKVFEEAKPNSELCCKPLCLMLADESDHETLTAILSPLIAEREAMKSSELMLELGGILRTFKFIFRGTGYDEKLVREVEGLEASGSVYICTLCDATRLEASQNLVFHSITRSHAENLERYEVWRSNPYHESVEELRDRVKGVSAKPFIETVPSIDALHCDIGNAAEFYKIFQLEIGEVYKNPNASKEERKRWQATLDKHLRKKMNLKPIMRMNGNFARKLMTKETVDAVCELIPSEERHEALRELMDLYLKMKPVWRSSCPAKECPESLCQYSFNSQRFAELLSTKFKYRYEGKITNYFHKTLAHVPEIIERDGSIGAWASEGNESGNKLFRRFRKMNARQSKCYEMEDVLKHHWLYTSKYLQKFMNAHNALKTSGFTMNPQASLGDPLGIEDSLESQDSMEF

Target ORF information:

RefSeq Version	XM_011520250.2
Organism	Homo sapiens(human)
Definition	Homo sapiens recombination activating 1 (RAG1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011520250.2

ORF Insert Sequence:

ATGGCAGCCT CTTTCCCACC CACCTTGGGA CTCAGTTCTG CCCCAGATGA AATTCAGCAC 
CCACATATTA AATTTTCAGA ATGGAAATTT AAGCTGTTCC GGGTGAGATC CTTTGAAAAG 
ACACCTGAAG AAGCTCAAAA GGAAAAGAAG GATTCCTTTG AGGGGAAACC CTCTCTGGAG 
CAATCTCCAG CAGTCCTGGA CAAGGCTGAT GGTCAGAAGC CAGTCCCAAC TCAGCCATTG 
TTAAAAGCCC ACCCTAAGTT TTCAAAGAAA TTTCACGACA ACGAGAAAGC AAGAGGCAAA 
GCGATCCATC AAGCCAACCT TCGACATCTC TGCCGCATCT GTGGGAATTC TTTTAGAGCT 
GATGAGCACA ACAGGAGATA TCCAGTCCAT GGTCCTGTGG ATGGTAAAAC CCTAGGCCTT 
TTACGAAAGA AGGAAAAGAG AGCTACTTCC TGGCCGGACC TCATTGCCAA GGTTTTCCGG 
ATCGATGTGA AGGCAGATGT TGACTCGATC CACCCCACTG AGTTCTGCCA TAACTGCTGG 
AGCATCATGC ACAGGAAGTT TAGCAGTGCC CCATGTGAGG TTTACTTCCC GAGGAACGTG 
ACCATGGAGT GGCACCCCCA CACACCATCC TGTGACATCT GCAACACTGC CCGTCGGGGA 
CTCAAGAGGA AGAGTCTTCA GCCAAACTTG CAGCTCAGCA AAAAACTCAA AACTGTGCTT 
GACCAAGCAA GACAAGCCCG TCAGCACAAG AGAAGAGCTC AGGCAAGGAT CAGCAGCAAG 
GATGTCATGA AGAAGATCGC CAACTGCAGT AAGATACATC TTAGTACCAA GCTCCTTGCA 
GTGGACTTCC CAGAGCACTT TGTGAAATCC ATCTCCTGCC AGATCTGTGA ACACATTCTG 
GCTGACCCTG TGGAGACCAA CTGTAAGCAT GTCTTTTGCC GGGTCTGCAT TCTCAGATGC 
CTCAAAGTCA TGGGCAGCTA TTGTCCCTCT TGCCGATATC CATGCTTCCC TACTGACCTG 
GAGAGTCCAG TGAAGTCCTT TCTGAGCGTC TTGAATTCCC TGATGGTGAA ATGTCCAGCA 
AAAGAGTGCA ATGAGGAGGT CAGTTTGGAA AAATATAATC ACCACATCTC AAGTCACAAG 
GAATCAAAAG AGATTTTTGT GCACATTAAT AAAGGGGGCC GGCCCCGCCA ACATCTTCTG 
TCGCTGACTC GGAGAGCTCA GAAGCACCGG CTGAGGGAGC TCAAGCTGCA AGTCAAAGCC 
TTTGCTGACA AAGAAGAAGG TGGAGATGTG AAGTCCGTGT GCATGACCTT GTTCCTGCTG 
GCTCTGAGGG CGAGGAATGA GCACAGGCAA GCTGATGAGC TGGAGGCCAT CATGCAGGGA 
AAGGGCTCTG GCCTGCAGCC AGCTGTTTGC TTGGCCATCC GTGTCAACAC CTTCCTCAGC 
TGCAGTCAGT ACCACAAGAT GTACAGGACT GTGAAAGCCA TCACAGGGAG ACAGATTTTT 
CAGCCTTTGC ATGCCCTTCG GAATGCTGAG AAGGTACTTC TGCCAGGCTA CCACCACTTT 
GAGTGGCAGC CACCTCTGAA GAATGTGTCT TCCAGCACTG ATGTTGGCAT TATTGATGGG 
CTGTCTGGAC TATCATCCTC TGTGGATGAT TACCCAGTGG ACACCATTGC AAAGAGGTTC 
CGCTATGATT CAGCTTTGGT GTCTGCTTTG ATGGACATGG AAGAAGACAT CTTGGAAGGC 
ATGAGATCCC AAGACCTTGA TGATTACCTG AATGGCCCCT TCACTGTGGT GGTGAAGGAG 
TCTTGTGATG GAATGGGAGA CGTGAGTGAG AAGCATGGGA GTGGGCCTGT AGTTCCAGAA 
AAGGCAGTCC GTTTTTCATT CACAATCATG AAAATTACTA TTGCCCACAG CTCTCAGAAT 
GTGAAAGTAT TTGAAGAAGC CAAACCTAAC TCTGAACTGT GTTGCAAGCC ATTGTGCCTT 
ATGCTGGCAG ATGAGTCTGA CCACGAGACG CTGACTGCCA TCCTGAGTCC TCTCATTGCT 
GAGAGGGAGG CCATGAAGAG CAGTGAATTA ATGCTTGAGC TGGGAGGCAT TCTCCGGACT 
TTCAAGTTCA TCTTCAGGGG CACCGGCTAT GATGAAAAAC TTGTGCGGGA AGTGGAAGGC 
CTCGAGGCTT CTGGCTCAGT CTACATTTGT ACTCTTTGTG ATGCCACCCG TCTGGAAGCC 
TCTCAAAATC TTGTCTTCCA CTCTATAACC AGAAGCCATG CTGAGAACCT GGAACGTTAT 
GAGGTCTGGC GTTCCAACCC TTACCATGAG TCTGTGGAAG AACTGCGGGA TCGGGTGAAA 
GGGGTCTCAG CTAAACCTTT CATTGAGACA GTCCCTTCCA TAGATGCACT CCACTGTGAC 
ATTGGCAATG CAGCTGAGTT CTACAAGATC TTCCAGCTAG AGATAGGGGA AGTGTATAAG 
AATCCCAATG CTTCCAAAGA GGAAAGGAAA AGGTGGCAGG CCACACTGGA CAAGCATCTC 
CGGAAGAAGA TGAACCTCAA ACCAATCATG AGGATGAATG GCAACTTTGC CAGGAAGCTC 
ATGACCAAAG AGACTGTGGA TGCAGTTTGT GAGTTAATTC CTTCCGAGGA GAGGCACGAG 
GCTCTGAGGG AGCTGATGGA TCTTTACCTG AAGATGAAAC CAGTATGGCG ATCATCATGC 
CCTGCTAAAG AGTGCCCAGA ATCCCTCTGC CAGTACAGTT TCAATTCACA GCGTTTTGCT 
GAGCTCCTTT CTACGAAGTT CAAGTATAGG TATGAGGGAA AAATCACCAA TTATTTTCAC 
AAAACCCTGG CCCATGTTCC TGAAATTATT GAGAGGGATG GCTCCATTGG GGCATGGGCA 
AGTGAGGGAA ATGAGTCTGG TAACAAACTG TTTAGGCGCT TCCGGAAAAT GAATGCCAGG 
CAGTCCAAAT GCTATGAGAT GGAAGATGTC CTGAAACACC ACTGGTTGTA CACCTCCAAA 
TACCTCCAGA AGTTTATGAA TGCTCATAAT GCATTAAAAA CCTCTGGGTT TACCATGAAC 
CCTCAGGCAA GCTTAGGGGA CCCATTAGGC ATAGAGGACT CTCTGGAAAG CCAAGATTCA 
ATGGAATTTT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Novel RAG1 mutation in a case of severe combined immunodeficiency.
Pediatrics116(3)e445-9(2005 Sep)
Zhang J,Quintal L,Atkinson A,Williams B,Grunebaum E,Roifman CM

The immunophenotypic and immunogenotypic B-cell differentiation arrest in bone marrow of RAG-deficient SCID patients corresponds to residual recombination activities of mutated RAG proteins.
Blood100(6)2145-52(2002 Sep)
Noordzij JG,de Bruin-Versteeg S,Verkaik NS,Vossen JM,de Groot R,Bernatowska E,Langerak AW,van Gent DC,van Dongen JJ

Identical mutations in RAG1 or RAG2 genes leading to defective V(D)J recombinase activity can cause either T-B-severe combined immune deficiency or Omenn syndrome.
Blood97(9)2772-6(2001 May)
Corneo B,Moshous D,G?ng?r T,Wulffraat N,Philippet P,Le Deist FL,Fischer A,de Villartay JP

V(D)J recombination defects in lymphocytes due to RAG mutations: severe immunodeficiency with a spectrum of clinical presentations.
Blood97(1)81-8(2001 Jan)
Villa A,Sobacchi C,Notarangelo LD,Bozzi F,Abinun M,Abrahamsen TG,Arkwright PD,Baniyash M,Brooks EG,Conley ME,Cortes P,Duse M,Fasth A,Filipovich AM,Infante AJ,Jones A,Mazzolari E,Muller SM,Pasic S,Rechavi G,Sacco MG,Santagata S,Schroeder ML,Seger R,Strina D,Ugazio A,V?liaho J,Vihinen M,Vogler LB,Ochs H,Vezzoni P,Friedrich W,Schwarz K

N-terminal RAG1 frameshift mutations in Omenn's syndrome: internal methionine usage leads to partial V(D)J recombination activity and reveals a fundamental role in vivo for the N-terminal domains.
Proceedings of the National Academy of Sciences of the United States of America97(26)14572-7(2000 Dec)
Santagata S,Gomez CA,Sobacchi C,Bozzi F,Abinun M,Pasic S,Cortes P,Vezzoni P,Villa A

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RAG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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