TRAPPC1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	TRAPPC1
Entrez Gene ID	58485
Full Name	trafficking protein particle complex 1
Synonyms	BET5,MUM2
General protein information	Preferred Names trafficking protein particle complex 1 Names trafficking protein particle complex subunit 1 BET5 homolog MUM-2 melanoma ubiquitous mutated 2 multiple myeloma protein 2
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17p13.1
Summary	This gene product plays a role in vesicular transport of proteins to the Golgi apparatus from the endoplasmic reticulum. The encoded protein is a component of the multisubunit transport protein particle (TRAPP) complex. Alternative splicing results in multiple transcript variants.[provided by RefSeq, Oct 2009].
Disorder MIM:	610969

mRNA and Protein(s)

mRNA	Protein	Name
NM_021210.4	NP_067033.1	trafficking protein particle complex subunit 1
NM_001166621.1	NP_001160093.1	trafficking protein particle complex subunit 1
NM_021210.5	NP_067033.1	trafficking protein particle complex subunit 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TRAPPC1	XP_001172364.1
Bos taurus (cattle)	TRAPPC1	NP_001069881.1
Mus musculus (house mouse)	Trappc1	NP_001019377.1
Rattus norvegicus (Norway rat)	Trappc1	NP_001034467.1
Canis lupus familiaris (dog)	TRAPPC1	XP_536628.2
Caenorhabditis elegans (roundworm)	CELE_DC2.8	NP_001122900.2
Magnaporthe oryzae (rice blast fungus)	MGG_06435	XP_003717134.1
Arabidopsis thaliana (thale cress)	AT1G51160	NP_175528.1
Oryza sativa (rice)	Os01g0513700	NP_001043184.1
Xenopus tropicalis (tropical clawed frog)	trappc1	NP_001008076.1
Homo sapiens (human)	TRAPPC1	NP_067033.1
	TRAPPC1	XP_001111285.1
Danio rerio (zebrafish)	trappc1	NP_001002742.1
Drosophila melanogaster (fruit fly)	Bet5	NP_651778.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000995	XP_309190.3
Schizosaccharomyces pombe (fission yeast)	bet5	NP_594073.1
Neurospora crassa	NCU06176	XP_962988.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Boldt K, van Reeuwijk J, Lu Q, Koutroumpas K, Nguyen TM, Texier Y, van Beersum SE, Horn N, Willer JR, Mans DA, Dougherty G, Lamers IJ, Coene KL, Arts HH, Betts MJ, Beyer T, Bolat E, Gloeckner CJ, Haidari K, Hetterschijt L, Iaconis D, Jenkins D, Klose F, Knapp B, Latour B, Letteboer SJ, Marcelis CL, Mitic D, Morleo M, Oud MM, Riemersma M, Rix S, Terhal PA, Toedt G, van Dam TJ, de Vrieze E, Wissinger Y, Wu KM, Apic G, Beales PL, Blacque OE, Gibson TJ, Huynen MA, Katsanis N, Kremer H, Omran H, van Wijk E, Wolfrum U, Kepes F, Davis EE, Franco B, Giles RH, Ueffing M, Russell RB, Roepman R,
Nature communications711491(2016 May)

Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.
Westlake CJ, Baye LM, Nachury MV, Wright KJ, Ervin KE, Phu L, Chalouni C, Beck JS, Kirkpatrick DS, Slusarski DC, Sheffield VC, Scheller RH, Jackson PK
Proceedings of the National Academy of Sciences of the United States of America108(7)2759-64(2011 Feb)

Structure of the Bet3-Tpc6B core of TRAPP: two Tpc6 paralogs form trimeric complexes with Bet3 and Mum2.
K?mmel D, M?ller JJ, Roske Y, Henke N, Heinemann U
Journal of molecular biology361(1)22-32(2006 Aug)

Multi-component protein complexes and Golgi membrane trafficking.
Oka T, Krieger M
Journal of biochemistry137(2)109-14(2005 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mum2 binds to the alpha1-alpha2 loop of the Bet3-Tpc6B heterodimer.
Title: Structure of the Bet3-Tpc6B core of TRAPP: two Tpc6 paralogs form trimeric complexes with Bet3 and Mum2.

This paper studies human as well as yeast TRAPP subunits.
Title: Functional organization of the yeast proteome by systematic analysis of protein complexes.

The following TRAPPC1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TRAPPC1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu01624	NM_001166621.1 Latest version!	Homo sapiens trafficking protein particle complex 1 (TRAPPC1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu01624	NM_021210.5 Latest version!	Homo sapiens trafficking protein particle complex 1 (TRAPPC1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu01624	NM_021210.4	Homo sapiens trafficking protein particle complex 1 (TRAPPC1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

TRAPPC1 ( NM_021210.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_067033.1 Homo sapiens trafficking protein particle complex 1 (TRAPPC1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01624
Clone ID Related Accession (Same CDS sequence)	NM_021210.4 , NM_001166621.1 , NM_021210.5
Accession Version	NM_021210.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-21
Organism	Homo sapiens(human)
Product	trafficking protein particle complex subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI457771.1, AC104581.22, BC032717.1 and BU687854.1. On Oct 31, 2009 this sequence version replaced NM_021210.3. Transcript Variant: This variant (1) represents the longest transcript. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: BU687854.1, BC032717.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGTCC ACAACCTGTA CCTGTTTGAC CGGAATGGAG TGTGTCTGCA CTACAGCGAA 
TGGCACCGCA AGAAGCAAGC AGGGATTCCC AAGGAGGAGG AGTATAAGCT GATGTACGGG 
ATGCTCTTCT CTATCCGCTC GTTTGTCAGC AAGATGTCCC CGCTAGACAT GAAGGATGGC 
TTCCTGGCCT TCCAAACTAG CCGTTACAAA CTCCATTACT ACGAGACGCC CACTGGGATC 
AAAGTTGTCA TGAATACTGA CTTGGGCGTG GGACCCATCC GAGATGTGCT GCACCACATC 
TACAGTGCGC TGTATGTGGA GCTGGTGGTG AAGAATCCCC TGTGCCCGCT GGGCCAAACT 
GTGCAAAGTG AGCTCTTTCG CTCCCGACTG GACTCCTATG TTCGCTCTCT GCCCTTCTTC 
TCCGCCCGGG CTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067033.1
CDS	121..558
Translation	MTVHNLYLFDRNGVCLHYSEWHRKKQAGIPKEEEYKLMYGMLFSIRSFVSKMSPLDMKDGFLAFQTSRYKLHYYETPTGIKVVMNTDLGVGPIRDVLHHIYSALYVELVVKNPLCPLGQTVQSELFRSRLDSYVRSLPFFSARAG

Target ORF information:

RefSeq Version	NM_021210.4
Organism	Homo sapiens(human)
Definition	Homo sapiens trafficking protein particle complex 1 (TRAPPC1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021210.4

ORF Insert Sequence:

ATGACTGTCC ACAACCTGTA CCTGTTTGAC CGGAATGGAG TGTGTCTGCA CTACAGCGAA 
TGGCACCGCA AGAAGCAAGC AGGGATTCCC AAGGAGGAGG AGTATAAGCT GATGTACGGG 
ATGCTCTTCT CTATCCGCTC GTTTGTCAGC AAGATGTCCC CGCTAGACAT GAAGGATGGC 
TTCCTGGCCT TCCAAACTAG CCGTTACAAA CTCCATTACT ACGAGACGCC CACTGGGATC 
AAAGTTGTCA TGAATACTGA CTTGGGCGTG GGACCCATCC GAGATGTGCT GCACCACATC 
TACAGTGCGC TGTATGTGGA GCTGGTGGTG AAGAATCCCC TGTGCCCGCT GGGCCAAACT 
GTGCAAAGTG AGCTCTTTCG CTCCCGACTG GACTCCTATG TTCGCTCTCT GCCCTTCTTC 
TCCGCCCGGG CTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRAPPC1 ( NM_001166621.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001160093.1 Homo sapiens trafficking protein particle complex 1 (TRAPPC1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu01624
Clone ID Related Accession (Same CDS sequence)	NM_021210.4 , NM_001166621.1 , NM_021210.5
Accession Version	NM_001166621.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	trafficking protein particle complex subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI600633.1, BM762006.1, BC032717.1 and BU687854.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR1163657.153007.1, SRR1163657.340691.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2156266, SAMEA2158188 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGACTGTCC ACAACCTGTA CCTGTTTGAC CGGAATGGAG TGTGTCTGCA CTACAGCGAA 
TGGCACCGCA AGAAGCAAGC AGGGATTCCC AAGGAGGAGG AGTATAAGCT GATGTACGGG 
ATGCTCTTCT CTATCCGCTC GTTTGTCAGC AAGATGTCCC CGCTAGACAT GAAGGATGGC 
TTCCTGGCCT TCCAAACTAG CCGTTACAAA CTCCATTACT ACGAGACGCC CACTGGGATC 
AAAGTTGTCA TGAATACTGA CTTGGGCGTG GGACCCATCC GAGATGTGCT GCACCACATC 
TACAGTGCGC TGTATGTGGA GCTGGTGGTG AAGAATCCCC TGTGCCCGCT GGGCCAAACT 
GTGCAAAGTG AGCTCTTTCG CTCCCGACTG GACTCCTATG TTCGCTCTCT GCCCTTCTTC 
TCCGCCCGGG CTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001160093.1
CDS	61..498
Translation	MTVHNLYLFDRNGVCLHYSEWHRKKQAGIPKEEEYKLMYGMLFSIRSFVSKMSPLDMKDGFLAFQTSRYKLHYYETPTGIKVVMNTDLGVGPIRDVLHHIYSALYVELVVKNPLCPLGQTVQSELFRSRLDSYVRSLPFFSARAG

Target ORF information:

RefSeq Version	NM_001166621.1
Organism	Homo sapiens(human)
Definition	Homo sapiens trafficking protein particle complex 1 (TRAPPC1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001166621.1

ORF Insert Sequence:

ATGACTGTCC ACAACCTGTA CCTGTTTGAC CGGAATGGAG TGTGTCTGCA CTACAGCGAA 
TGGCACCGCA AGAAGCAAGC AGGGATTCCC AAGGAGGAGG AGTATAAGCT GATGTACGGG 
ATGCTCTTCT CTATCCGCTC GTTTGTCAGC AAGATGTCCC CGCTAGACAT GAAGGATGGC 
TTCCTGGCCT TCCAAACTAG CCGTTACAAA CTCCATTACT ACGAGACGCC CACTGGGATC 
AAAGTTGTCA TGAATACTGA CTTGGGCGTG GGACCCATCC GAGATGTGCT GCACCACATC 
TACAGTGCGC TGTATGTGGA GCTGGTGGTG AAGAATCCCC TGTGCCCGCT GGGCCAAACT 
GTGCAAAGTG AGCTCTTTCG CTCCCGACTG GACTCCTATG TTCGCTCTCT GCCCTTCTTC 
TCCGCCCGGG CTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TRAPPC1 ( NM_021210.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_067033.1 Homo sapiens trafficking protein particle complex 1 (TRAPPC1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu01624
Clone ID Related Accession (Same CDS sequence)	NM_021210.4 , NM_001166621.1 , NM_021210.5
Accession Version	NM_021210.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 438bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	trafficking protein particle complex subunit 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC104581.22 and BC032717.1. On May 17, 2019 this sequence version replaced NM_021210.4. Transcript Variant: This variant (1) represents the longest transcript. Both variants 1 and 2 encode the same protein. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.165702.1, SRR3476690.124091.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000303731.9/ ENSP00000302783.4 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGACTGTCC ACAACCTGTA CCTGTTTGAC CGGAATGGAG TGTGTCTGCA CTACAGCGAA 
TGGCACCGCA AGAAGCAAGC AGGGATTCCC AAGGAGGAGG AGTATAAGCT GATGTACGGG 
ATGCTCTTCT CTATCCGCTC GTTTGTCAGC AAGATGTCCC CGCTAGACAT GAAGGATGGC 
TTCCTGGCCT TCCAAACTAG CCGTTACAAA CTCCATTACT ACGAGACGCC CACTGGGATC 
AAAGTTGTCA TGAATACTGA CTTGGGCGTG GGACCCATCC GAGATGTGCT GCACCACATC 
TACAGTGCGC TGTATGTGGA GCTGGTGGTG AAGAATCCCC TGTGCCCGCT GGGCCAAACT 
GTGCAAAGTG AGCTCTTTCG CTCCCGACTG GACTCCTATG TTCGCTCTCT GCCCTTCTTC 
TCCGCCCGGG CTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067033.1
CDS	118..555
Translation	MTVHNLYLFDRNGVCLHYSEWHRKKQAGIPKEEEYKLMYGMLFSIRSFVSKMSPLDMKDGFLAFQTSRYKLHYYETPTGIKVVMNTDLGVGPIRDVLHHIYSALYVELVVKNPLCPLGQTVQSELFRSRLDSYVRSLPFFSARAG

Target ORF information:

RefSeq Version	NM_021210.5
Organism	Homo sapiens(human)
Definition	Homo sapiens trafficking protein particle complex 1 (TRAPPC1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021210.5

ORF Insert Sequence:

ATGACTGTCC ACAACCTGTA CCTGTTTGAC CGGAATGGAG TGTGTCTGCA CTACAGCGAA 
TGGCACCGCA AGAAGCAAGC AGGGATTCCC AAGGAGGAGG AGTATAAGCT GATGTACGGG 
ATGCTCTTCT CTATCCGCTC GTTTGTCAGC AAGATGTCCC CGCTAGACAT GAAGGATGGC 
TTCCTGGCCT TCCAAACTAG CCGTTACAAA CTCCATTACT ACGAGACGCC CACTGGGATC 
AAAGTTGTCA TGAATACTGA CTTGGGCGTG GGACCCATCC GAGATGTGCT GCACCACATC 
TACAGTGCGC TGTATGTGGA GCTGGTGGTG AAGAATCCCC TGTGCCCGCT GGGCCAAACT 
GTGCAAAGTG AGCTCTTTCG CTCCCGACTG GACTCCTATG TTCGCTCTCT GCCCTTCTTC 
TCCGCCCGGG CTGGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

An organelle-specific protein landscape identifies novel diseases and molecular mechanisms.
Nature communications711491(2016 May)
Boldt K,van Reeuwijk J,Lu Q,Koutroumpas K,Nguyen TM,Texier Y,van Beersum SE,Horn N,Willer JR,Mans DA,Dougherty G,Lamers IJ,Coene KL,Arts HH,Betts MJ,Beyer T,Bolat E,Gloeckner CJ,Haidari K,Hetterschijt L,Iaconis D,Jenkins D,Klose F,Knapp B,Latour B,Letteboer SJ,Marcelis CL,Mitic D,Morleo M,Oud MM,Riemersma M,Rix S,Terhal PA,Toedt G,van Dam TJ,de Vrieze E,Wissinger Y,Wu KM,Apic G,Beales PL,Blacque OE,Gibson TJ,Huynen MA,Katsanis N,Kremer H,Omran H,van Wijk E,Wolfrum U,Kepes F,Davis EE,Franco B,Giles RH,Ueffing M,Russell RB,Roepman R,

Primary cilia membrane assembly is initiated by Rab11 and transport protein particle II (TRAPPII) complex-dependent trafficking of Rabin8 to the centrosome.
Proceedings of the National Academy of Sciences of the United States of America108(7)2759-64(2011 Feb)
Westlake CJ,Baye LM,Nachury MV,Wright KJ,Ervin KE,Phu L,Chalouni C,Beck JS,Kirkpatrick DS,Slusarski DC,Sheffield VC,Scheller RH,Jackson PK

Structure of the Bet3-Tpc6B core of TRAPP: two Tpc6 paralogs form trimeric complexes with Bet3 and Mum2.
Journal of molecular biology361(1)22-32(2006 Aug)
K?mmel D,M?ller JJ,Roske Y,Henke N,Heinemann U

Multi-component protein complexes and Golgi membrane trafficking.
Journal of biochemistry137(2)109-14(2005 Feb)
Oka T,Krieger M

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TRAPPC1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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