RHOU cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	RHOU
Entrez Gene ID	58480
Full Name	ras homolog family member U
Synonyms	ARHU,CDC42L1,G28K,WRCH1,hG28K
General protein information	Preferred Names ras homolog family member U Names rho-related GTP-binding protein RhoU 2310026M05Rik CDC42-like GTPase 1 GTP-binding protein SB128 GTP-binding protein like 1 Ryu GTPase ras homolog gene family, member U ras-like gene family member U rho GTPase-like protein ARHU wnt-1 responsive Cdc42 homolog 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 1 Map Location: 1q42.13
Summary	This gene encodes a member of the Rho family of GTPases. This protein can activate PAK1 and JNK1, and can induce filopodium formation and stress fiber dissolution. It may also mediate the effects of WNT1 signaling in the regulation of cell morphology, cytoskeletal organization, and cell proliferation. A non-coding transcript variant of this gene results from naturally occurring read-through transcription between this locus and the neighboring DUSP5P (dual specificity phosphatase 5 pseudogene) locus.[provided by RefSeq, Mar 2011].
Disorder MIM:	606366

mRNA and Protein(s)

mRNA	Protein	Name
NM_021205.5	NP_067028.1	rho-related GTP-binding protein RhoU
NM_021205.6	NP_067028.1	rho-related GTP-binding protein RhoU

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	RHOU	XP_426146.4
Homo sapiens (human)	RHOU	NP_067028.1
Canis lupus familiaris (dog)	RHOU	XP_003639029.1
Mus musculus (house mouse)	Rhou	NP_598716.1
Xenopus tropicalis (tropical clawed frog)	rhou	NP_001016852.1
	RHOU	XP_001083463.1
Bos taurus (cattle)	RHOU	NP_001091616.1
Danio rerio (zebrafish)	rhoua	NP_001007444.1

Gene Ontology
Bibliography

Related articles in PubMed

PAK4 promotes kinase-independent stabilization of RhoU to modulate cell adhesion.
Dart AE, Box GM, Court W, Gale ME, Brown JP, Pinder SE, Eccles SA, Wells CM
The Journal of cell biology211(4)863-79(2015 Nov)

Atypical RhoV and RhoU GTPases control development of the neural crest.
Faure S, Fort P
Small GTPases6(4)174-7(2015 Oct)

Germline sequence variants in TGM3 and RGS22 confer risk of basal cell carcinoma.
Stacey SN, Sulem P, Gudbjartsson DF, Jonasdottir A, Thorleifsson G, Gudjonsson SA, Masson G, Gudmundsson J, Sigurgeirsson B, Benediktsdottir KR, Thorisdottir K, Ragnarsson R, Fuentelsaz V, Corredera C, Grasa M, Planelles D, Sanmartin O, Rudnai P, Gurzau E, Koppova K, Hemminki K, Nex? BA, Tj?nneland A, Overvad K, Johannsdottir H, Helgadottir HT, Thorsteinsdottir U, Kong A, Vogel U, Kumar R, Nagore E, Mayordomo JI, Rafnar T, Olafsson JH, Stefansson K
Human molecular genetics23(11)3045-53(2014 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PAK4 and RhoU cooperate to drive adhesion turnover and promote cell migration.
Title: PAK4 promotes kinase-independent stabilization of RhoU to modulate cell adhesion.

This review addresses the developmental roles of 2 GTPases of the Rho family, RhoV/Chp and RhoU/Wrch.
Title: Atypical RhoV and RhoU GTPases control development of the neural crest.

Data indicate that upregulated miR-126 upon coxsackievirus B3 (CVB3) infection targets SPRED1, LRP6, and WRCH1 genes, mediating cross-talk between ERK1/2 and Wnt/beta-catenin pathways, and thus promoting viral replication.
Title: MiR-126 promotes coxsackievirus replication by mediating cross-talk of ERK1/2 and Wnt/?-catenin signal pathways.

multiple upstream signals may converge on Wrch1 directly through its SH3 domain-binding properties
Title: SH3-mediated targeting of Wrch1/RhoU by multiple adaptor proteins.

results indicate that NOTCH1 mutation stimulates T-cell acute lymphoblastic leukaemia cell migration through RhoU upregulation that could contribute to the leukaemia cell dissemination
Title: Analysis of Rho GTPase expression in T-ALL identifies RhoU as a target for Notch involved in T-ALL cell migration.

The following RHOU gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RHOU cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu22557	NM_021205.6 Latest version!	Homo sapiens ras homolog family member U (RHOU), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
View Old Accession Versions >>
OHu22557	NM_021205.5	Homo sapiens ras homolog family member U (RHOU), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

RHOU ( NM_021205.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_067028.1 Homo sapiens ras homolog family member U (RHOU), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22557
Clone ID Related Accession (Same CDS sequence)	NM_021205.5 , NM_021205.6
Accession Version	NM_021205.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	rho-related GTP-binding protein RhoU
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL096776.12, AF211836.1, BC040076.1 and AI094830.1. On Mar 15, 2011 this sequence version replaced NM_021205.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF211836.1, BC040076.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCCCCGC AGCAGGGGGA CCCCGCGTTC CCCGACCGCT GCGAGGCGCC TCCGGTGCCG 
CCGCGTCGGG AGCGCGGTGG ACGCGGGGGA CGCGGGCCTG GGGAGCCGGG GGGCCGGGGG 
CGTGCGGGGG GTGCCGAGGG GCGCGGCGTC AAGTGCGTGC TGGTCGGCGA CGGCGCGGTG 
GGCAAGACGA GCCTGGTGGT GAGCTACACC ACCAACGGCT ACCCCACCGA GTACATCCCT 
ACTGCCTTCG ACAACTTCTC CGCGGTGGTG TCTGTGGATG GGCGGCCCGT GAGACTCCAA 
CTCTGTGACA CTGCCGGACA GGATGAATTT GACAAGCTGA GGCCTCTCTG CTACACCAAC 
ACAGACATCT TCCTGCTCTG CTTCAGTGTC GTGAGCCCCT CATCCTTCCA GAACGTCAGT 
GAGAAATGGG TGCCGGAGAT TCGATGCCAC TGTCCCAAAG CCCCCATCAT CCTAGTTGGA 
ACGCAGTCGG ATCTCAGAGA AGATGTCAAA GTCCTCATTG AGTTGGACAA ATGCAAAGAA 
AAGCCAGTGC CTGAAGAGGC GGCTAAGCTG TGCGCCGAGG AAATCAAAGC CGCCTCCTAC 
ATCGAGTGTT CAGCCTTGAC TCAAAAAAAC CTCAAAGAGG TCTTTGATGC AGCCATCGTC 
GCTGGCATTC AATACTCGGA CACTCAGCAA CAGCCAAAGA AGTCTAAAAG CAGGACTCCA 
GATAAAATGA AAAACCTCTC CAAGTCCTGG TGGAAGAAGT ACTGCTGTTT CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067028.1
CDS	667..1443
Translation	MPPQQGDPAFPDRCEAPPVPPRRERGGRGGRGPGEPGGRGRAGGAEGRGVKCVLVGDGAVGKTSLVVSYTTNGYPTEYIPTAFDNFSAVVSVDGRPVRLQLCDTAGQDEFDKLRPLCYTNTDIFLLCFSVVSPSSFQNVSEKWVPEIRCHCPKAPIILVGTQSDLREDVKVLIELDKCKEKPVPEEAAKLCAEEIKAASYIECSALTQKNLKEVFDAAIVAGIQYSDTQQQPKKSKSRTPDKMKNLSKSWWKKYCCFV

Target ORF information:

RefSeq Version	NM_021205.5
Organism	Homo sapiens(human)
Definition	Homo sapiens ras homolog family member U (RHOU), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021205.5

ORF Insert Sequence:

ATGCCCCCGC AGCAGGGGGA CCCCGCGTTC CCCGACCGCT GCGAGGCGCC TCCGGTGCCG 
CCGCGTCGGG AGCGCGGTGG ACGCGGGGGA CGCGGGCCTG GGGAGCCGGG GGGCCGGGGG 
CGTGCGGGGG GTGCCGAGGG GCGCGGCGTC AAGTGCGTGC TGGTCGGCGA CGGCGCGGTG 
GGCAAGACGA GCCTGGTGGT GAGCTACACC ACCAACGGCT ACCCCACCGA GTACATCCCT 
ACTGCCTTCG ACAACTTCTC CGCGGTGGTG TCTGTGGATG GGCGGCCCGT GAGACTCCAA 
CTCTGTGACA CTGCCGGACA GGATGAATTT GACAAGCTGA GGCCTCTCTG CTACACCAAC 
ACAGACATCT TCCTGCTCTG CTTCAGTGTC GTGAGCCCCT CATCCTTCCA GAACGTCAGT 
GAGAAATGGG TGCCGGAGAT TCGATGCCAC TGTCCCAAAG CCCCCATCAT CCTAGTTGGA 
ACGCAGTCGG ATCTCAGAGA AGATGTCAAA GTCCTCATTG AGTTGGACAA ATGCAAAGAA 
AAGCCAGTGC CTGAAGAGGC GGCTAAGCTG TGCGCCGAGG AAATCAAAGC CGCCTCCTAC 
ATCGAGTGTT CAGCCTTGAC TCAAAAAAAC CTCAAAGAGG TCTTTGATGC AGCCATCGTC 
GCTGGCATTC AATACTCGGA CACTCAGCAA CAGCCAAAGA AGTCTAAAAG CAGGACTCCA 
GATAAAATGA AAAACCTCTC CAAGTCCTGG TGGAAGAAGT ACTGCTGTTT CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RHOU ( NM_021205.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_067028.1 Homo sapiens ras homolog family member U (RHOU), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu22557
Clone ID Related Accession (Same CDS sequence)	NM_021205.5 , NM_021205.6
Accession Version	NM_021205.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 777bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	rho-related GTP-binding protein RhoU
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF211836.1 and BC040076.1. On Nov 23, 2018 this sequence version replaced NM_021205.5. Transcript Variant: This variant (1) represents the longer transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040076.1, AF211836.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000366691.4/ ENSP00000355652.3 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCCCCCGC AGCAGGGGGA CCCCGCGTTC CCCGACCGCT GCGAGGCGCC TCCGGTGCCG 
CCGCGTCGGG AGCGCGGTGG ACGCGGGGGA CGCGGGCCTG GGGAGCCGGG GGGCCGGGGG 
CGTGCGGGGG GTGCCGAGGG GCGCGGCGTC AAGTGCGTGC TGGTCGGCGA CGGCGCGGTG 
GGCAAGACGA GCCTGGTGGT GAGCTACACC ACCAACGGCT ACCCCACCGA GTACATCCCT 
ACTGCCTTCG ACAACTTCTC CGCGGTGGTG TCTGTGGATG GGCGGCCCGT GAGACTCCAA 
CTCTGTGACA CTGCCGGACA GGATGAATTT GACAAGCTGA GGCCTCTCTG CTACACCAAC 
ACAGACATCT TCCTGCTCTG CTTCAGTGTC GTGAGCCCCT CATCCTTCCA GAACGTCAGT 
GAGAAATGGG TGCCGGAGAT TCGATGCCAC TGTCCCAAAG CCCCCATCAT CCTAGTTGGA 
ACGCAGTCGG ATCTCAGAGA AGATGTCAAA GTCCTCATTG AGTTGGACAA ATGCAAAGAA 
AAGCCAGTGC CTGAAGAGGC GGCTAAGCTG TGCGCCGAGG AAATCAAAGC CGCCTCCTAC 
ATCGAGTGTT CAGCCTTGAC TCAAAAAAAC CTCAAAGAGG TCTTTGATGC AGCCATCGTC 
GCTGGCATTC AATACTCGGA CACTCAGCAA CAGCCAAAGA AGTCTAAAAG CAGGACTCCA 
GATAAAATGA AAAACCTCTC CAAGTCCTGG TGGAAGAAGT ACTGCTGTTT CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_067028.1
CDS	265..1041
Translation	MPPQQGDPAFPDRCEAPPVPPRRERGGRGGRGPGEPGGRGRAGGAEGRGVKCVLVGDGAVGKTSLVVSYTTNGYPTEYIPTAFDNFSAVVSVDGRPVRLQLCDTAGQDEFDKLRPLCYTNTDIFLLCFSVVSPSSFQNVSEKWVPEIRCHCPKAPIILVGTQSDLREDVKVLIELDKCKEKPVPEEAAKLCAEEIKAASYIECSALTQKNLKEVFDAAIVAGIQYSDTQQQPKKSKSRTPDKMKNLSKSWWKKYCCFV

Target ORF information:

RefSeq Version	NM_021205.6
Organism	Homo sapiens(human)
Definition	Homo sapiens ras homolog family member U (RHOU), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021205.6

ORF Insert Sequence:

ATGCCCCCGC AGCAGGGGGA CCCCGCGTTC CCCGACCGCT GCGAGGCGCC TCCGGTGCCG 
CCGCGTCGGG AGCGCGGTGG ACGCGGGGGA CGCGGGCCTG GGGAGCCGGG GGGCCGGGGG 
CGTGCGGGGG GTGCCGAGGG GCGCGGCGTC AAGTGCGTGC TGGTCGGCGA CGGCGCGGTG 
GGCAAGACGA GCCTGGTGGT GAGCTACACC ACCAACGGCT ACCCCACCGA GTACATCCCT 
ACTGCCTTCG ACAACTTCTC CGCGGTGGTG TCTGTGGATG GGCGGCCCGT GAGACTCCAA 
CTCTGTGACA CTGCCGGACA GGATGAATTT GACAAGCTGA GGCCTCTCTG CTACACCAAC 
ACAGACATCT TCCTGCTCTG CTTCAGTGTC GTGAGCCCCT CATCCTTCCA GAACGTCAGT 
GAGAAATGGG TGCCGGAGAT TCGATGCCAC TGTCCCAAAG CCCCCATCAT CCTAGTTGGA 
ACGCAGTCGG ATCTCAGAGA AGATGTCAAA GTCCTCATTG AGTTGGACAA ATGCAAAGAA 
AAGCCAGTGC CTGAAGAGGC GGCTAAGCTG TGCGCCGAGG AAATCAAAGC CGCCTCCTAC 
ATCGAGTGTT CAGCCTTGAC TCAAAAAAAC CTCAAAGAGG TCTTTGATGC AGCCATCGTC 
GCTGGCATTC AATACTCGGA CACTCAGCAA CAGCCAAAGA AGTCTAAAAG CAGGACTCCA 
GATAAAATGA AAAACCTCTC CAAGTCCTGG TGGAAGAAGT ACTGCTGTTT CGTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

PAK4 promotes kinase-independent stabilization of RhoU to modulate cell adhesion.
The Journal of cell biology211(4)863-79(2015 Nov)
Dart AE,Box GM,Court W,Gale ME,Brown JP,Pinder SE,Eccles SA,Wells CM

Atypical RhoV and RhoU GTPases control development of the neural crest.
Small GTPases6(4)174-7(2015 Oct)
Faure S,Fort P

Germline sequence variants in TGM3 and RGS22 confer risk of basal cell carcinoma.
Human molecular genetics23(11)3045-53(2014 Jun)
Stacey SN,Sulem P,Gudbjartsson DF,Jonasdottir A,Thorleifsson G,Gudjonsson SA,Masson G,Gudmundsson J,Sigurgeirsson B,Benediktsdottir KR,Thorisdottir K,Ragnarsson R,Fuentelsaz V,Corredera C,Grasa M,Planelles D,Sanmartin O,Rudnai P,Gurzau E,Koppova K,Hemminki K,Nex? BA,Tj?nneland A,Overvad K,Johannsdottir H,Helgadottir HT,Thorsteinsdottir U,Kong A,Vogel U,Kumar R,Nagore E,Mayordomo JI,Rafnar T,Olafsson JH,Stefansson K

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RHOU cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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