PYGL cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PYGL
Entrez Gene ID	5836
Full Name	glycogen phosphorylase L
Synonyms	GSD6
General protein information	Preferred Names glycogen phosphorylase L Names glycogen phosphorylase, liver form phosphorylase, glycogen, liver
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q22.1
Summary	This gene encodes a homodimeric protein that catalyses the cleavage of alpha-1,4-glucosidic bonds to release glucose-1-phosphate from liver glycogen stores. This protein switches from inactive phosphorylase B to active phosphorylase A by phosphorylation of serine residue 15. Activity of this enzyme is further regulated by multiple allosteric effectors and hormonal controls. Humans have three glycogen phosphorylase genes that encode distinct isozymes that are primarily expressed in liver, brain and muscle, respectively. The liver isozyme serves the glycemic demands of the body in general while the brain and muscle isozymes supply just those tissues. In glycogen storage disease type VI, also known as Hers disease, mutations in liver glycogen phosphorylase inhibit the conversion of glycogen to glucose and results in moderate hypoglycemia, mild ketosis, growth retardation and hepatomegaly. Alternative splicing results in multiple transcript variants encoding different isoforms.[provided by RefSeq, Feb 2011].
Disorder MIM:	613741
Disorder Html:	Glycogen storage disease VI, 232700 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_002863.4	NP_002854.3	glycogen phosphorylase, liver form isoform 1
NM_001163940.1	NP_001157412.1	glycogen phosphorylase, liver form isoform 2
NM_001163940.2	NP_001157412.1	glycogen phosphorylase, liver form isoform 2
NM_002863.5	NP_002854.3	glycogen phosphorylase, liver form isoform 1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PYGL	NP_002854.3
Gallus gallus (chicken)	PYGL	NP_989723.1
Pan troglodytes (chimpanzee)	PYGL	XP_001145433.2
Mus musculus (house mouse)	Pygl	NP_573461.2
Saccharomyces cerevisiae (baker's yeast)	GPH1	NP_015486.1
Eremothecium gossypii	AGOS_AFR547W	NP_986094.1
Arabidopsis thaliana (thale cress)	AT3G29320	NP_189578.1
Magnaporthe oryzae (rice blast fungus)	MGG_01819	XP_003714833.1
Neurospora crassa	NCU07027	XP_962166.2
	PYGL	XP_001102253.1
Xenopus tropicalis (tropical clawed frog)	pygl	XP_002936619.1
Canis lupus familiaris (dog)	PYGL	XP_003639242.1
Bos taurus (cattle)	PYGL	NP_001068671.1
Rattus norvegicus (Norway rat)	Pygl	NP_071604.1
Kluyveromyces lactis	KLLA0F10065g	XP_455538.1

Gene Ontology
Bibliography

Related articles in PubMed

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Genome-wide association study of atypical psychosis.
Kanazawa T, Ikeda M, Glatt SJ, Tsutsumi A, Kikuyama H, Kawamura Y, Nishida N, Miyagawa T, Hashimoto R, Takeda M, Sasaki T, Tokunaga K, Koh J, Iwata N, Yoneda H
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics162B(7)679-86(2013 Oct)

Genome-wide association study identifies germline polymorphisms associated with relapse of childhood acute lymphoblastic leukemia.
Yang JJ, Cheng C, Devidas M, Cao X, Campana D, Yang W, Fan Y, Neale G, Cox N, Scheet P, Borowitz MJ, Winick NJ, Martin PL, Bowman WP, Camitta B, Reaman GH, Carroll WL, Willman CL, Hunger SP, Evans WE, Pui CH, Loh M, Relling MV
Blood120(20)4197-204(2012 Nov)

Acetylation negatively regulates glycogen phosphorylase by recruiting protein phosphatase 1.
Zhang T, Wang S, Lin Y, Xu W, Ye D, Xiong Y, Zhao S, Guan KL
Cell metabolism15(1)75-87(2012 Jan)

Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.
Flachsbart F, Franke A, Kleindorp R, Caliebe A, Blanch? H, Schreiber S, Nebel A
Mutation research694(1-2)13-9(2010 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Observational study of gene-disease association. (HuGE Navigator)
Title: Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.

Clinical trial of gene-disease association and gene-environment interaction. (HuGE Navigator)
Title: Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score.

Deficiency of liver glycogen phosphorylase is predominantly the result of missense mutations affecting enzyme activity. There are no common mutations and the severity of clinical symptoms varies significantly.
Title: High frequency of missense mutations in glycogen storage disease type VI.

Susceptibility to excessive liver glycogen storage in patients with type 1 diabetes.
Title: Intermittent and recurrent hepatomegaly due to glycogen storage in a patient with type 1 diabetes: genetic analysis of the liver glycogen phosphorylase gene (PYGL).

The following PYGL gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PYGL cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu19505	NM_002863.5 Latest version!	Homo sapiens glycogen phosphorylase L (PYGL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu16439	NM_001163940.2 Latest version!	Homo sapiens glycogen phosphorylase L (PYGL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$580.30 $829.00
View Old Accession Versions >>
OHu16439	NM_001163940.1	Homo sapiens glycogen phosphorylase L (PYGL), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$580.30 $829.00
OHu19505	NM_002863.4	Homo sapiens glycogen phosphorylase L (PYGL), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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Reference Sequences (Refseq)

PYGL ( NM_002863.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002854.3 Homo sapiens glycogen phosphorylase L (PYGL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19505
Clone ID Related Accession (Same CDS sequence)	NM_002863.4 , NM_002863.5
Accession Version	NM_002863.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	glycogen phosphorylase, liver form isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA287767.1, BC095850.1 and AA523002.1. This sequence is a reference standard in the RefSeqGene project. On Aug 7, 2009 this sequence version replaced NM_002863.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.106036.1, M14636.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAAGC CCCTGACGGA CCAGGAGAAG CGGCGGCAGA TCAGCATCCG CGGCATCGTG 
GGCGTGGAGA ACGTGGCAGA GCTGAAGAAG AGTTTCAACC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACCACCCGC GACTACTACT TCGCGCTGGC GCACACGGTG 
CGCGACCACC TGGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTACGA CAAGTGCCCC 
AAGAGGGTAT ATTACCTCTC TCTGGAATTT TACATGGGCC GAACATTACA GAACACCATG 
ATCAACCTCG GTCTGCAAAA TGCCTGTGAT GAGGCCATTT ACCAGCTTGG ATTGGATATA 
GAAGAGTTAG AAGAAATTGA AGAAGATGCT GGACTTGGCA ATGGTGGTCT TGGGAGACTT 
GCTGCCTGCT TCTTGGATTC CATGGCAACC CTGGGACTTG CAGCCTATGG ATACGGCATT 
CGGTATGAAT ATGGGATTTT CAATCAGAAG ATCCGAGATG GATGGCAGGT AGAAGAAGCA 
GATGATTGGC TCAGATATGG AAACCCTTGG GAGAAGTCCC GCCCAGAATT CATGCTGCCT 
GTGCACTTCT ATGGAAAAGT AGAACACACC AACACCGGGA CCAAGTGGAT TGACACTCAA 
GTGGTCCTGG CTCTGCCATA TGACACCCCC GTGCCCGGCT ACATGAATAA CACTGTCAAC 
ACCATGCGCC TCTGGTCTGC TCGGGCACCA AATGACTTTA ACCTCAGAGA CTTTAATGTT 
GGAGACTACA TTCAGGCTGT GCTGGACCGA AACCTGGCCG AGAACATCTC CCGGGTCCTC 
TATCCCAATG ACAATTTTTT TGAAGGGAAG GAGCTAAGAT TGAAGCAGGA ATACTTTGTG 
GTGGCTGCAA CCTTGCAAGA TATCATCCGC CGTTTCAAAG CCTCCAAGTT TGGCTCCACC 
CGTGGTGCAG GAACTGTGTT TGATGCCTTC CCGGATCAGG TGGCCATCCA GCTGAATGAC 
ACTCACCCTG CACTCGCGAT CCCTGAGCTG ATGAGGATTT TTGTGGATAT TGAAAAACTG 
CCCTGGTCCA AGGCATGGGA GCTCACCCAG AAGACCTTCG CCTACACCAA CCACACAGTG 
CTCCCGGAAG CCCTGGAGCG CTGGCCCGTG GACCTGGTGG AGAAGCTGCT CCCTCGACAT 
TTGGAAATCA TTTATGAGAT AAATCAGAAG CATTTAGATA GAATTGTGGC CTTGTTTCCT 
AAAGATGTGG ACCGTCTGAG AAGGATGTCT CTGATAGAAG AGGAAGGAAG CAAAAGGATC 
AACATGGCCC ATCTCTGCAT TGTCGGTTCC CATGCTGTGA ATGGCGTGGC TAAAATCCAC 
TCAGACATCG TGAAGACTAA AGTATTCAAG GACTTCAGTG AGCTAGAACC TGACAAGTTT 
CAGAATAAAA CCAATGGGAT CACTCCAAGG CGCTGGCTCC TACTCTGCAA CCCAGGACTT 
GCAGAGCTCA TAGCAGAGAA AATTGGAGAA GACTATGTGA AAGACCTGAG CCAGCTGACG 
AAGCTCCACA GCTTCCTGGG TGATGATGTC TTCCTCCGGG AACTCGCCAA GGTGAAGCAG 
GAGAATAAGC TGAAGTTTTC TCAGTTCCTG GAGACGGAGT ACAAAGTGAA GATCAACCCA 
TCCTCCATGT TTGATGTCCA GGTGAAGAGG ATACATGAGT ACAAGCGACA GCTCTTGAAC 
TGTCTGCATG TGATCACGAT GTACAACCGC ATTAAGAAAG ACCCTAAGAA GTTATTCGTG 
CCAAGGACAG TTATCATTGG TGGTAAAGCT GCCCCAGGAT ATCACATGGC CAAAATGATC 
ATAAAGCTGA TCACTTCAGT GGCAGATGTG GTGAACAATG ACCCTATGGT TGGAAGCAAG 
TTGAAAGTCA TCTTCTTGGA GAACTACAGA GTATCTCTTG CTGAAAAAGT CATTCCAGCC 
ACAGATCTGT CAGAGCAGAT TTCCACTGCA GGCACCGAAG CCTCGGGGAC AGGCAATATG 
AAGTTCATGC TAAATGGGGC CCTAACTATC GGGACCATGG ATGGGGCCAA TGTGGAAATG 
GCAGAAGAAG CTGGGGAAGA GAACCTGTTC ATCTTTGGCA TGAGGATAGA TGATGTGGCT 
GCTTTGGACA AGAAAGGGTA CGAGGCAAAA GAATACTATG AGGCACTTCC AGAGCTGAAG 
CTGGTCATTG ATCAAATTGA CAATGGCTTT TTTTCTCCCA AGCAGCCTGA CCTCTTCAAA 
GATATCATCA ACATGCTATT TTATCATGAC AGGTTTAAAG TCTTTGCAGA CTACGAAGCC 
TATGTCAAGT GTCAAGATAA AGTGAGTCAG CTGTACATGA ATCCAAAGGC CTGGAACACA 
ATGGTACTCA AAAACATAGC TGCCTCGGGG AAATTCTCCA GTGACCGAAC AATTAAAGAA 
TATGCCCAAA ACATCTGGAA CGTGGAACCT TCAGATCTAA AGATTTCTCT ATCCAATGAA 
TCTAACAAAG TCAATGGAAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002854.3
CDS	128..2671
Translation	MAKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTLVKDRNVATTRDYYFALAHTVRDHLVGRWIRTQQHYYDKCPKRVYYLSLEFYMGRTLQNTMINLGLQNACDEAIYQLGLDIEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEYGIFNQKIRDGWQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHTNTGTKWIDTQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLRDFNVGDYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDLVEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLIEEEGSKRINMAHLCIVGSHAVNGVAKIHSDIVKTKVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKIGEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDVQVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNESNKVNGN

Target ORF information:

RefSeq Version	NM_002863.4
Organism	Homo sapiens(human)
Definition	Homo sapiens glycogen phosphorylase L (PYGL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002863.4

ORF Insert Sequence:

ATGGCGAAGC CCCTGACGGA CCAGGAGAAG CGGCGGCAGA TCAGCATCCG CGGCATCGTG 
GGCGTGGAGA ACGTGGCAGA GCTGAAGAAG AGTTTCAACC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACCACCCGC GACTACTACT TCGCGCTGGC GCACACGGTG 
CGCGACCACC TGGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTACGA CAAGTGCCCC 
AAGAGGGTAT ATTACCTCTC TCTGGAATTT TACATGGGCC GAACATTACA GAACACCATG 
ATCAACCTCG GTCTGCAAAA TGCCTGTGAT GAGGCCATTT ACCAGCTTGG ATTGGATATA 
GAAGAGTTAG AAGAAATTGA AGAAGATGCT GGACTTGGCA ATGGTGGTCT TGGGAGACTT 
GCTGCCTGCT TCTTGGATTC CATGGCAACC CTGGGACTTG CAGCCTATGG ATACGGCATT 
CGGTATGAAT ATGGGATTTT CAATCAGAAG ATCCGAGATG GATGGCAGGT AGAAGAAGCA 
GATGATTGGC TCAGATATGG AAACCCTTGG GAGAAGTCCC GCCCAGAATT CATGCTGCCT 
GTGCACTTCT ATGGAAAAGT AGAACACACC AACACCGGGA CCAAGTGGAT TGACACTCAA 
GTGGTCCTGG CTCTGCCATA TGACACCCCC GTGCCCGGCT ACATGAATAA CACTGTCAAC 
ACCATGCGCC TCTGGTCTGC TCGGGCACCA AATGACTTTA ACCTCAGAGA CTTTAATGTT 
GGAGACTACA TTCAGGCTGT GCTGGACCGA AACCTGGCCG AGAACATCTC CCGGGTCCTC 
TATCCCAATG ACAATTTTTT TGAAGGGAAG GAGCTAAGAT TGAAGCAGGA ATACTTTGTG 
GTGGCTGCAA CCTTGCAAGA TATCATCCGC CGTTTCAAAG CCTCCAAGTT TGGCTCCACC 
CGTGGTGCAG GAACTGTGTT TGATGCCTTC CCGGATCAGG TGGCCATCCA GCTGAATGAC 
ACTCACCCTG CACTCGCGAT CCCTGAGCTG ATGAGGATTT TTGTGGATAT TGAAAAACTG 
CCCTGGTCCA AGGCATGGGA GCTCACCCAG AAGACCTTCG CCTACACCAA CCACACAGTG 
CTCCCGGAAG CCCTGGAGCG CTGGCCCGTG GACCTGGTGG AGAAGCTGCT CCCTCGACAT 
TTGGAAATCA TTTATGAGAT AAATCAGAAG CATTTAGATA GAATTGTGGC CTTGTTTCCT 
AAAGATGTGG ACCGTCTGAG AAGGATGTCT CTGATAGAAG AGGAAGGAAG CAAAAGGATC 
AACATGGCCC ATCTCTGCAT TGTCGGTTCC CATGCTGTGA ATGGCGTGGC TAAAATCCAC 
TCAGACATCG TGAAGACTAA AGTATTCAAG GACTTCAGTG AGCTAGAACC TGACAAGTTT 
CAGAATAAAA CCAATGGGAT CACTCCAAGG CGCTGGCTCC TACTCTGCAA CCCAGGACTT 
GCAGAGCTCA TAGCAGAGAA AATTGGAGAA GACTATGTGA AAGACCTGAG CCAGCTGACG 
AAGCTCCACA GCTTCCTGGG TGATGATGTC TTCCTCCGGG AACTCGCCAA GGTGAAGCAG 
GAGAATAAGC TGAAGTTTTC TCAGTTCCTG GAGACGGAGT ACAAAGTGAA GATCAACCCA 
TCCTCCATGT TTGATGTCCA GGTGAAGAGG ATACATGAGT ACAAGCGACA GCTCTTGAAC 
TGTCTGCATG TGATCACGAT GTACAACCGC ATTAAGAAAG ACCCTAAGAA GTTATTCGTG 
CCAAGGACAG TTATCATTGG TGGTAAAGCT GCCCCAGGAT ATCACATGGC CAAAATGATC 
ATAAAGCTGA TCACTTCAGT GGCAGATGTG GTGAACAATG ACCCTATGGT TGGAAGCAAG 
TTGAAAGTCA TCTTCTTGGA GAACTACAGA GTATCTCTTG CTGAAAAAGT CATTCCAGCC 
ACAGATCTGT CAGAGCAGAT TTCCACTGCA GGCACCGAAG CCTCGGGGAC AGGCAATATG 
AAGTTCATGC TAAATGGGGC CCTAACTATC GGGACCATGG ATGGGGCCAA TGTGGAAATG 
GCAGAAGAAG CTGGGGAAGA GAACCTGTTC ATCTTTGGCA TGAGGATAGA TGATGTGGCT 
GCTTTGGACA AGAAAGGGTA CGAGGCAAAA GAATACTATG AGGCACTTCC AGAGCTGAAG 
CTGGTCATTG ATCAAATTGA CAATGGCTTT TTTTCTCCCA AGCAGCCTGA CCTCTTCAAA 
GATATCATCA ACATGCTATT TTATCATGAC AGGTTTAAAG TCTTTGCAGA CTACGAAGCC 
TATGTCAAGT GTCAAGATAA AGTGAGTCAG CTGTACATGA ATCCAAAGGC CTGGAACACA 
ATGGTACTCA AAAACATAGC TGCCTCGGGG AAATTCTCCA GTGACCGAAC AATTAAAGAA 
TATGCCCAAA ACATCTGGAA CGTGGAACCT TCAGATCTAA AGATTTCTCT ATCCAATGAA 
TCTAACAAAG TCAATGGAAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PYGL ( NM_002863.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002854.3 Homo sapiens glycogen phosphorylase L (PYGL), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu19505
Clone ID Related Accession (Same CDS sequence)	NM_002863.4 , NM_002863.5
Accession Version	NM_002863.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2544bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	glycogen phosphorylase, liver form isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC095850.1 and AA523002.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_002863.4. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK225904.1, BC095850.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000216392.8/ ENSP00000216392.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGAAGC CCCTGACGGA CCAGGAGAAG CGGCGGCAGA TCAGCATCCG CGGCATCGTG 
GGCGTGGAGA ACGTGGCAGA GCTGAAGAAG AGTTTCAACC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACCACCCGC GACTACTACT TCGCGCTGGC GCACACGGTG 
CGCGACCACC TGGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTACGA CAAGTGCCCC 
AAGAGGGTAT ATTACCTCTC TCTGGAATTT TACATGGGCC GAACATTACA GAACACCATG 
ATCAACCTCG GTCTGCAAAA TGCCTGTGAT GAGGCCATTT ACCAGCTTGG ATTGGATATA 
GAAGAGTTAG AAGAAATTGA AGAAGATGCT GGACTTGGCA ATGGTGGTCT TGGGAGACTT 
GCTGCCTGCT TCTTGGATTC CATGGCAACC CTGGGACTTG CAGCCTATGG ATACGGCATT 
CGGTATGAAT ATGGGATTTT CAATCAGAAG ATCCGAGATG GATGGCAGGT AGAAGAAGCA 
GATGATTGGC TCAGATATGG AAACCCTTGG GAGAAGTCCC GCCCAGAATT CATGCTGCCT 
GTGCACTTCT ATGGAAAAGT AGAACACACC AACACCGGGA CCAAGTGGAT TGACACTCAA 
GTGGTCCTGG CTCTGCCATA TGACACCCCC GTGCCCGGCT ACATGAATAA CACTGTCAAC 
ACCATGCGCC TCTGGTCTGC TCGGGCACCA AATGACTTTA ACCTCAGAGA CTTTAATGTT 
GGAGACTACA TTCAGGCTGT GCTGGACCGA AACCTGGCCG AGAACATCTC CCGGGTCCTC 
TATCCCAATG ACAATTTTTT TGAAGGGAAG GAGCTAAGAT TGAAGCAGGA ATACTTTGTG 
GTGGCTGCAA CCTTGCAAGA TATCATCCGC CGTTTCAAAG CCTCCAAGTT TGGCTCCACC 
CGTGGTGCAG GAACTGTGTT TGATGCCTTC CCGGATCAGG TGGCCATCCA GCTGAATGAC 
ACTCACCCTG CACTCGCGAT CCCTGAGCTG ATGAGGATTT TTGTGGATAT TGAAAAACTG 
CCCTGGTCCA AGGCATGGGA GCTCACCCAG AAGACCTTCG CCTACACCAA CCACACAGTG 
CTCCCGGAAG CCCTGGAGCG CTGGCCCGTG GACCTGGTGG AGAAGCTGCT CCCTCGACAT 
TTGGAAATCA TTTATGAGAT AAATCAGAAG CATTTAGATA GAATTGTGGC CTTGTTTCCT 
AAAGATGTGG ACCGTCTGAG AAGGATGTCT CTGATAGAAG AGGAAGGAAG CAAAAGGATC 
AACATGGCCC ATCTCTGCAT TGTCGGTTCC CATGCTGTGA ATGGCGTGGC TAAAATCCAC 
TCAGACATCG TGAAGACTAA AGTATTCAAG GACTTCAGTG AGCTAGAACC TGACAAGTTT 
CAGAATAAAA CCAATGGGAT CACTCCAAGG CGCTGGCTCC TACTCTGCAA CCCAGGACTT 
GCAGAGCTCA TAGCAGAGAA AATTGGAGAA GACTATGTGA AAGACCTGAG CCAGCTGACG 
AAGCTCCACA GCTTCCTGGG TGATGATGTC TTCCTCCGGG AACTCGCCAA GGTGAAGCAG 
GAGAATAAGC TGAAGTTTTC TCAGTTCCTG GAGACGGAGT ACAAAGTGAA GATCAACCCA 
TCCTCCATGT TTGATGTCCA GGTGAAGAGG ATACATGAGT ACAAGCGACA GCTCTTGAAC 
TGTCTGCATG TGATCACGAT GTACAACCGC ATTAAGAAAG ACCCTAAGAA GTTATTCGTG 
CCAAGGACAG TTATCATTGG TGGTAAAGCT GCCCCAGGAT ATCACATGGC CAAAATGATC 
ATAAAGCTGA TCACTTCAGT GGCAGATGTG GTGAACAATG ACCCTATGGT TGGAAGCAAG 
TTGAAAGTCA TCTTCTTGGA GAACTACAGA GTATCTCTTG CTGAAAAAGT CATTCCAGCC 
ACAGATCTGT CAGAGCAGAT TTCCACTGCA GGCACCGAAG CCTCGGGGAC AGGCAATATG 
AAGTTCATGC TAAATGGGGC CCTAACTATC GGGACCATGG ATGGGGCCAA TGTGGAAATG 
GCAGAAGAAG CTGGGGAAGA GAACCTGTTC ATCTTTGGCA TGAGGATAGA TGATGTGGCT 
GCTTTGGACA AGAAAGGGTA CGAGGCAAAA GAATACTATG AGGCACTTCC AGAGCTGAAG 
CTGGTCATTG ATCAAATTGA CAATGGCTTT TTTTCTCCCA AGCAGCCTGA CCTCTTCAAA 
GATATCATCA ACATGCTATT TTATCATGAC AGGTTTAAAG TCTTTGCAGA CTACGAAGCC 
TATGTCAAGT GTCAAGATAA AGTGAGTCAG CTGTACATGA ATCCAAAGGC CTGGAACACA 
ATGGTACTCA AAAACATAGC TGCCTCGGGG AAATTCTCCA GTGACCGAAC AATTAAAGAA 
TATGCCCAAA ACATCTGGAA CGTGGAACCT TCAGATCTAA AGATTTCTCT ATCCAATGAA 
TCTAACAAAG TCAATGGAAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002854.3
CDS	81..2624
Translation	MAKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTLVKDRNVATTRDYYFALAHTVRDHLVGRWIRTQQHYYDKCPKRVYYLSLEFYMGRTLQNTMINLGLQNACDEAIYQLGLDIEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEYGIFNQKIRDGWQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHTNTGTKWIDTQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLRDFNVGDYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDLVEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLIEEEGSKRINMAHLCIVGSHAVNGVAKIHSDIVKTKVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKIGEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDVQVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNESNKVNGN

Target ORF information:

RefSeq Version	NM_002863.5
Organism	Homo sapiens(human)
Definition	Homo sapiens glycogen phosphorylase L (PYGL), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002863.5

ORF Insert Sequence:

ATGGCGAAGC CCCTGACGGA CCAGGAGAAG CGGCGGCAGA TCAGCATCCG CGGCATCGTG 
GGCGTGGAGA ACGTGGCAGA GCTGAAGAAG AGTTTCAACC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACCACCCGC GACTACTACT TCGCGCTGGC GCACACGGTG 
CGCGACCACC TGGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTACGA CAAGTGCCCC 
AAGAGGGTAT ATTACCTCTC TCTGGAATTT TACATGGGCC GAACATTACA GAACACCATG 
ATCAACCTCG GTCTGCAAAA TGCCTGTGAT GAGGCCATTT ACCAGCTTGG ATTGGATATA 
GAAGAGTTAG AAGAAATTGA AGAAGATGCT GGACTTGGCA ATGGTGGTCT TGGGAGACTT 
GCTGCCTGCT TCTTGGATTC CATGGCAACC CTGGGACTTG CAGCCTATGG ATACGGCATT 
CGGTATGAAT ATGGGATTTT CAATCAGAAG ATCCGAGATG GATGGCAGGT AGAAGAAGCA 
GATGATTGGC TCAGATATGG AAACCCTTGG GAGAAGTCCC GCCCAGAATT CATGCTGCCT 
GTGCACTTCT ATGGAAAAGT AGAACACACC AACACCGGGA CCAAGTGGAT TGACACTCAA 
GTGGTCCTGG CTCTGCCATA TGACACCCCC GTGCCCGGCT ACATGAATAA CACTGTCAAC 
ACCATGCGCC TCTGGTCTGC TCGGGCACCA AATGACTTTA ACCTCAGAGA CTTTAATGTT 
GGAGACTACA TTCAGGCTGT GCTGGACCGA AACCTGGCCG AGAACATCTC CCGGGTCCTC 
TATCCCAATG ACAATTTTTT TGAAGGGAAG GAGCTAAGAT TGAAGCAGGA ATACTTTGTG 
GTGGCTGCAA CCTTGCAAGA TATCATCCGC CGTTTCAAAG CCTCCAAGTT TGGCTCCACC 
CGTGGTGCAG GAACTGTGTT TGATGCCTTC CCGGATCAGG TGGCCATCCA GCTGAATGAC 
ACTCACCCTG CACTCGCGAT CCCTGAGCTG ATGAGGATTT TTGTGGATAT TGAAAAACTG 
CCCTGGTCCA AGGCATGGGA GCTCACCCAG AAGACCTTCG CCTACACCAA CCACACAGTG 
CTCCCGGAAG CCCTGGAGCG CTGGCCCGTG GACCTGGTGG AGAAGCTGCT CCCTCGACAT 
TTGGAAATCA TTTATGAGAT AAATCAGAAG CATTTAGATA GAATTGTGGC CTTGTTTCCT 
AAAGATGTGG ACCGTCTGAG AAGGATGTCT CTGATAGAAG AGGAAGGAAG CAAAAGGATC 
AACATGGCCC ATCTCTGCAT TGTCGGTTCC CATGCTGTGA ATGGCGTGGC TAAAATCCAC 
TCAGACATCG TGAAGACTAA AGTATTCAAG GACTTCAGTG AGCTAGAACC TGACAAGTTT 
CAGAATAAAA CCAATGGGAT CACTCCAAGG CGCTGGCTCC TACTCTGCAA CCCAGGACTT 
GCAGAGCTCA TAGCAGAGAA AATTGGAGAA GACTATGTGA AAGACCTGAG CCAGCTGACG 
AAGCTCCACA GCTTCCTGGG TGATGATGTC TTCCTCCGGG AACTCGCCAA GGTGAAGCAG 
GAGAATAAGC TGAAGTTTTC TCAGTTCCTG GAGACGGAGT ACAAAGTGAA GATCAACCCA 
TCCTCCATGT TTGATGTCCA GGTGAAGAGG ATACATGAGT ACAAGCGACA GCTCTTGAAC 
TGTCTGCATG TGATCACGAT GTACAACCGC ATTAAGAAAG ACCCTAAGAA GTTATTCGTG 
CCAAGGACAG TTATCATTGG TGGTAAAGCT GCCCCAGGAT ATCACATGGC CAAAATGATC 
ATAAAGCTGA TCACTTCAGT GGCAGATGTG GTGAACAATG ACCCTATGGT TGGAAGCAAG 
TTGAAAGTCA TCTTCTTGGA GAACTACAGA GTATCTCTTG CTGAAAAAGT CATTCCAGCC 
ACAGATCTGT CAGAGCAGAT TTCCACTGCA GGCACCGAAG CCTCGGGGAC AGGCAATATG 
AAGTTCATGC TAAATGGGGC CCTAACTATC GGGACCATGG ATGGGGCCAA TGTGGAAATG 
GCAGAAGAAG CTGGGGAAGA GAACCTGTTC ATCTTTGGCA TGAGGATAGA TGATGTGGCT 
GCTTTGGACA AGAAAGGGTA CGAGGCAAAA GAATACTATG AGGCACTTCC AGAGCTGAAG 
CTGGTCATTG ATCAAATTGA CAATGGCTTT TTTTCTCCCA AGCAGCCTGA CCTCTTCAAA 
GATATCATCA ACATGCTATT TTATCATGAC AGGTTTAAAG TCTTTGCAGA CTACGAAGCC 
TATGTCAAGT GTCAAGATAA AGTGAGTCAG CTGTACATGA ATCCAAAGGC CTGGAACACA 
ATGGTACTCA AAAACATAGC TGCCTCGGGG AAATTCTCCA GTGACCGAAC AATTAAAGAA 
TATGCCCAAA ACATCTGGAA CGTGGAACCT TCAGATCTAA AGATTTCTCT ATCCAATGAA 
TCTAACAAAG TCAATGGAAA TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PYGL ( NM_001163940.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157412.1 Homo sapiens glycogen phosphorylase L (PYGL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16439
Clone ID Related Accession (Same CDS sequence)	NM_001163940.1 , NM_001163940.2
Accession Version	NM_001163940.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	glycogen phosphorylase, liver form isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA287767.1, BC095850.1 and AA523002.1. Transcript Variant: This variant (2) lacks in an in-frame exon in the 5' coding region, compared to variant 1, which results in a shorter isoform (2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300580.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAAGC CCCTGACGGA CCAGGAGAAG CGGCGGCAGA TCAGCATCCG CGGCATCGTG 
GGCGTGGAGA ACGTGGCAGA GCTGAAGAAG AGTTTCAACC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACCACCCGC GACTACTACT TCGCGCTGGC GCACACGGTG 
CGCGACCACC TGGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTACGA CAAGTGCCCC 
AAGCTTGGAT TGGATATAGA AGAGTTAGAA GAAATTGAAG AAGATGCTGG ACTTGGCAAT 
GGTGGTCTTG GGAGACTTGC TGCCTGCTTC TTGGATTCCA TGGCAACCCT GGGACTTGCA 
GCCTATGGAT ACGGCATTCG GTATGAATAT GGGATTTTCA ATCAGAAGAT CCGAGATGGA 
TGGCAGGTAG AAGAAGCAGA TGATTGGCTC AGATATGGAA ACCCTTGGGA GAAGTCCCGC 
CCAGAATTCA TGCTGCCTGT GCACTTCTAT GGAAAAGTAG AACACACCAA CACCGGGACC 
AAGTGGATTG ACACTCAAGT GGTCCTGGCT CTGCCATATG ACACCCCCGT GCCCGGCTAC 
ATGAATAACA CTGTCAACAC CATGCGCCTC TGGTCTGCTC GGGCACCAAA TGACTTTAAC 
CTCAGAGACT TTAATGTTGG AGACTACATT CAGGCTGTGC TGGACCGAAA CCTGGCCGAG 
AACATCTCCC GGGTCCTCTA TCCCAATGAC AATTTTTTTG AAGGGAAGGA GCTAAGATTG 
AAGCAGGAAT ACTTTGTGGT GGCTGCAACC TTGCAAGATA TCATCCGCCG TTTCAAAGCC 
TCCAAGTTTG GCTCCACCCG TGGTGCAGGA ACTGTGTTTG ATGCCTTCCC GGATCAGGTG 
GCCATCCAGC TGAATGACAC TCACCCTGCA CTCGCGATCC CTGAGCTGAT GAGGATTTTT 
GTGGATATTG AAAAACTGCC CTGGTCCAAG GCATGGGAGC TCACCCAGAA GACCTTCGCC 
TACACCAACC ACACAGTGCT CCCGGAAGCC CTGGAGCGCT GGCCCGTGGA CCTGGTGGAG 
AAGCTGCTCC CTCGACATTT GGAAATCATT TATGAGATAA ATCAGAAGCA TTTAGATAGA 
ATTGTGGCCT TGTTTCCTAA AGATGTGGAC CGTCTGAGAA GGATGTCTCT GATAGAAGAG 
GAAGGAAGCA AAAGGATCAA CATGGCCCAT CTCTGCATTG TCGGTTCCCA TGCTGTGAAT 
GGCGTGGCTA AAATCCACTC AGACATCGTG AAGACTAAAG TATTCAAGGA CTTCAGTGAG 
CTAGAACCTG ACAAGTTTCA GAATAAAACC AATGGGATCA CTCCAAGGCG CTGGCTCCTA 
CTCTGCAACC CAGGACTTGC AGAGCTCATA GCAGAGAAAA TTGGAGAAGA CTATGTGAAA 
GACCTGAGCC AGCTGACGAA GCTCCACAGC TTCCTGGGTG ATGATGTCTT CCTCCGGGAA 
CTCGCCAAGG TGAAGCAGGA GAATAAGCTG AAGTTTTCTC AGTTCCTGGA GACGGAGTAC 
AAAGTGAAGA TCAACCCATC CTCCATGTTT GATGTCCAGG TGAAGAGGAT ACATGAGTAC 
AAGCGACAGC TCTTGAACTG TCTGCATGTG ATCACGATGT ACAACCGCAT TAAGAAAGAC 
CCTAAGAAGT TATTCGTGCC AAGGACAGTT ATCATTGGTG GTAAAGCTGC CCCAGGATAT 
CACATGGCCA AAATGATCAT AAAGCTGATC ACTTCAGTGG CAGATGTGGT GAACAATGAC 
CCTATGGTTG GAAGCAAGTT GAAAGTCATC TTCTTGGAGA ACTACAGAGT ATCTCTTGCT 
GAAAAAGTCA TTCCAGCCAC AGATCTGTCA GAGCAGATTT CCACTGCAGG CACCGAAGCC 
TCGGGGACAG GCAATATGAA GTTCATGCTA AATGGGGCCC TAACTATCGG GACCATGGAT 
GGGGCCAATG TGGAAATGGC AGAAGAAGCT GGGGAAGAGA ACCTGTTCAT CTTTGGCATG 
AGGATAGATG ATGTGGCTGC TTTGGACAAG AAAGGGTACG AGGCAAAAGA ATACTATGAG 
GCACTTCCAG AGCTGAAGCT GGTCATTGAT CAAATTGACA ATGGCTTTTT TTCTCCCAAG 
CAGCCTGACC TCTTCAAAGA TATCATCAAC ATGCTATTTT ATCATGACAG GTTTAAAGTC 
TTTGCAGACT ACGAAGCCTA TGTCAAGTGT CAAGATAAAG TGAGTCAGCT GTACATGAAT 
CCAAAGGCCT GGAACACAAT GGTACTCAAA AACATAGCTG CCTCGGGGAA ATTCTCCAGT 
GACCGAACAA TTAAAGAATA TGCCCAAAAC ATCTGGAACG TGGAACCTTC AGATCTAAAG 
ATTTCTCTAT CCAATGAATC TAACAAAGTC AATGGAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157412.1
CDS	128..2569
Translation	MAKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTLVKDRNVATTRDYYFALAHTVRDHLVGRWIRTQQHYYDKCPKLGLDIEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEYGIFNQKIRDGWQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHTNTGTKWIDTQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLRDFNVGDYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDLVEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLIEEEGSKRINMAHLCIVGSHAVNGVAKIHSDIVKTKVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKIGEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDVQVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNESNKVNGN

Target ORF information:

RefSeq Version	NM_001163940.1
Organism	Homo sapiens(human)
Definition	Homo sapiens glycogen phosphorylase L (PYGL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163940.1

ORF Insert Sequence:

ATGGCGAAGC CCCTGACGGA CCAGGAGAAG CGGCGGCAGA TCAGCATCCG CGGCATCGTG 
GGCGTGGAGA ACGTGGCAGA GCTGAAGAAG AGTTTCAACC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACCACCCGC GACTACTACT TCGCGCTGGC GCACACGGTG 
CGCGACCACC TGGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTACGA CAAGTGCCCC 
AAGCTTGGAT TGGATATAGA AGAGTTAGAA GAAATTGAAG AAGATGCTGG ACTTGGCAAT 
GGTGGTCTTG GGAGACTTGC TGCCTGCTTC TTGGATTCCA TGGCAACCCT GGGACTTGCA 
GCCTATGGAT ACGGCATTCG GTATGAATAT GGGATTTTCA ATCAGAAGAT CCGAGATGGA 
TGGCAGGTAG AAGAAGCAGA TGATTGGCTC AGATATGGAA ACCCTTGGGA GAAGTCCCGC 
CCAGAATTCA TGCTGCCTGT GCACTTCTAT GGAAAAGTAG AACACACCAA CACCGGGACC 
AAGTGGATTG ACACTCAAGT GGTCCTGGCT CTGCCATATG ACACCCCCGT GCCCGGCTAC 
ATGAATAACA CTGTCAACAC CATGCGCCTC TGGTCTGCTC GGGCACCAAA TGACTTTAAC 
CTCAGAGACT TTAATGTTGG AGACTACATT CAGGCTGTGC TGGACCGAAA CCTGGCCGAG 
AACATCTCCC GGGTCCTCTA TCCCAATGAC AATTTTTTTG AAGGGAAGGA GCTAAGATTG 
AAGCAGGAAT ACTTTGTGGT GGCTGCAACC TTGCAAGATA TCATCCGCCG TTTCAAAGCC 
TCCAAGTTTG GCTCCACCCG TGGTGCAGGA ACTGTGTTTG ATGCCTTCCC GGATCAGGTG 
GCCATCCAGC TGAATGACAC TCACCCTGCA CTCGCGATCC CTGAGCTGAT GAGGATTTTT 
GTGGATATTG AAAAACTGCC CTGGTCCAAG GCATGGGAGC TCACCCAGAA GACCTTCGCC 
TACACCAACC ACACAGTGCT CCCGGAAGCC CTGGAGCGCT GGCCCGTGGA CCTGGTGGAG 
AAGCTGCTCC CTCGACATTT GGAAATCATT TATGAGATAA ATCAGAAGCA TTTAGATAGA 
ATTGTGGCCT TGTTTCCTAA AGATGTGGAC CGTCTGAGAA GGATGTCTCT GATAGAAGAG 
GAAGGAAGCA AAAGGATCAA CATGGCCCAT CTCTGCATTG TCGGTTCCCA TGCTGTGAAT 
GGCGTGGCTA AAATCCACTC AGACATCGTG AAGACTAAAG TATTCAAGGA CTTCAGTGAG 
CTAGAACCTG ACAAGTTTCA GAATAAAACC AATGGGATCA CTCCAAGGCG CTGGCTCCTA 
CTCTGCAACC CAGGACTTGC AGAGCTCATA GCAGAGAAAA TTGGAGAAGA CTATGTGAAA 
GACCTGAGCC AGCTGACGAA GCTCCACAGC TTCCTGGGTG ATGATGTCTT CCTCCGGGAA 
CTCGCCAAGG TGAAGCAGGA GAATAAGCTG AAGTTTTCTC AGTTCCTGGA GACGGAGTAC 
AAAGTGAAGA TCAACCCATC CTCCATGTTT GATGTCCAGG TGAAGAGGAT ACATGAGTAC 
AAGCGACAGC TCTTGAACTG TCTGCATGTG ATCACGATGT ACAACCGCAT TAAGAAAGAC 
CCTAAGAAGT TATTCGTGCC AAGGACAGTT ATCATTGGTG GTAAAGCTGC CCCAGGATAT 
CACATGGCCA AAATGATCAT AAAGCTGATC ACTTCAGTGG CAGATGTGGT GAACAATGAC 
CCTATGGTTG GAAGCAAGTT GAAAGTCATC TTCTTGGAGA ACTACAGAGT ATCTCTTGCT 
GAAAAAGTCA TTCCAGCCAC AGATCTGTCA GAGCAGATTT CCACTGCAGG CACCGAAGCC 
TCGGGGACAG GCAATATGAA GTTCATGCTA AATGGGGCCC TAACTATCGG GACCATGGAT 
GGGGCCAATG TGGAAATGGC AGAAGAAGCT GGGGAAGAGA ACCTGTTCAT CTTTGGCATG 
AGGATAGATG ATGTGGCTGC TTTGGACAAG AAAGGGTACG AGGCAAAAGA ATACTATGAG 
GCACTTCCAG AGCTGAAGCT GGTCATTGAT CAAATTGACA ATGGCTTTTT TTCTCCCAAG 
CAGCCTGACC TCTTCAAAGA TATCATCAAC ATGCTATTTT ATCATGACAG GTTTAAAGTC 
TTTGCAGACT ACGAAGCCTA TGTCAAGTGT CAAGATAAAG TGAGTCAGCT GTACATGAAT 
CCAAAGGCCT GGAACACAAT GGTACTCAAA AACATAGCTG CCTCGGGGAA ATTCTCCAGT 
GACCGAACAA TTAAAGAATA TGCCCAAAAC ATCTGGAACG TGGAACCTTC AGATCTAAAG 
ATTTCTCTAT CCAATGAATC TAACAAAGTC AATGGAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PYGL ( NM_001163940.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001157412.1 Homo sapiens glycogen phosphorylase L (PYGL), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu16439
Clone ID Related Accession (Same CDS sequence)	NM_001163940.1 , NM_001163940.2
Accession Version	NM_001163940.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-03
Organism	Homo sapiens(human)
Product	glycogen phosphorylase, liver form isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC095850.1 and AA523002.1. On May 31, 2019 this sequence version replaced NM_001163940.1. Transcript Variant: This variant (2) lacks in an in-frame exon in the 5' coding region, compared to variant 1, which results in a shorter isoform (2), compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300580.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAAGC CCCTGACGGA CCAGGAGAAG CGGCGGCAGA TCAGCATCCG CGGCATCGTG 
GGCGTGGAGA ACGTGGCAGA GCTGAAGAAG AGTTTCAACC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACCACCCGC GACTACTACT TCGCGCTGGC GCACACGGTG 
CGCGACCACC TGGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTACGA CAAGTGCCCC 
AAGCTTGGAT TGGATATAGA AGAGTTAGAA GAAATTGAAG AAGATGCTGG ACTTGGCAAT 
GGTGGTCTTG GGAGACTTGC TGCCTGCTTC TTGGATTCCA TGGCAACCCT GGGACTTGCA 
GCCTATGGAT ACGGCATTCG GTATGAATAT GGGATTTTCA ATCAGAAGAT CCGAGATGGA 
TGGCAGGTAG AAGAAGCAGA TGATTGGCTC AGATATGGAA ACCCTTGGGA GAAGTCCCGC 
CCAGAATTCA TGCTGCCTGT GCACTTCTAT GGAAAAGTAG AACACACCAA CACCGGGACC 
AAGTGGATTG ACACTCAAGT GGTCCTGGCT CTGCCATATG ACACCCCCGT GCCCGGCTAC 
ATGAATAACA CTGTCAACAC CATGCGCCTC TGGTCTGCTC GGGCACCAAA TGACTTTAAC 
CTCAGAGACT TTAATGTTGG AGACTACATT CAGGCTGTGC TGGACCGAAA CCTGGCCGAG 
AACATCTCCC GGGTCCTCTA TCCCAATGAC AATTTTTTTG AAGGGAAGGA GCTAAGATTG 
AAGCAGGAAT ACTTTGTGGT GGCTGCAACC TTGCAAGATA TCATCCGCCG TTTCAAAGCC 
TCCAAGTTTG GCTCCACCCG TGGTGCAGGA ACTGTGTTTG ATGCCTTCCC GGATCAGGTG 
GCCATCCAGC TGAATGACAC TCACCCTGCA CTCGCGATCC CTGAGCTGAT GAGGATTTTT 
GTGGATATTG AAAAACTGCC CTGGTCCAAG GCATGGGAGC TCACCCAGAA GACCTTCGCC 
TACACCAACC ACACAGTGCT CCCGGAAGCC CTGGAGCGCT GGCCCGTGGA CCTGGTGGAG 
AAGCTGCTCC CTCGACATTT GGAAATCATT TATGAGATAA ATCAGAAGCA TTTAGATAGA 
ATTGTGGCCT TGTTTCCTAA AGATGTGGAC CGTCTGAGAA GGATGTCTCT GATAGAAGAG 
GAAGGAAGCA AAAGGATCAA CATGGCCCAT CTCTGCATTG TCGGTTCCCA TGCTGTGAAT 
GGCGTGGCTA AAATCCACTC AGACATCGTG AAGACTAAAG TATTCAAGGA CTTCAGTGAG 
CTAGAACCTG ACAAGTTTCA GAATAAAACC AATGGGATCA CTCCAAGGCG CTGGCTCCTA 
CTCTGCAACC CAGGACTTGC AGAGCTCATA GCAGAGAAAA TTGGAGAAGA CTATGTGAAA 
GACCTGAGCC AGCTGACGAA GCTCCACAGC TTCCTGGGTG ATGATGTCTT CCTCCGGGAA 
CTCGCCAAGG TGAAGCAGGA GAATAAGCTG AAGTTTTCTC AGTTCCTGGA GACGGAGTAC 
AAAGTGAAGA TCAACCCATC CTCCATGTTT GATGTCCAGG TGAAGAGGAT ACATGAGTAC 
AAGCGACAGC TCTTGAACTG TCTGCATGTG ATCACGATGT ACAACCGCAT TAAGAAAGAC 
CCTAAGAAGT TATTCGTGCC AAGGACAGTT ATCATTGGTG GTAAAGCTGC CCCAGGATAT 
CACATGGCCA AAATGATCAT AAAGCTGATC ACTTCAGTGG CAGATGTGGT GAACAATGAC 
CCTATGGTTG GAAGCAAGTT GAAAGTCATC TTCTTGGAGA ACTACAGAGT ATCTCTTGCT 
GAAAAAGTCA TTCCAGCCAC AGATCTGTCA GAGCAGATTT CCACTGCAGG CACCGAAGCC 
TCGGGGACAG GCAATATGAA GTTCATGCTA AATGGGGCCC TAACTATCGG GACCATGGAT 
GGGGCCAATG TGGAAATGGC AGAAGAAGCT GGGGAAGAGA ACCTGTTCAT CTTTGGCATG 
AGGATAGATG ATGTGGCTGC TTTGGACAAG AAAGGGTACG AGGCAAAAGA ATACTATGAG 
GCACTTCCAG AGCTGAAGCT GGTCATTGAT CAAATTGACA ATGGCTTTTT TTCTCCCAAG 
CAGCCTGACC TCTTCAAAGA TATCATCAAC ATGCTATTTT ATCATGACAG GTTTAAAGTC 
TTTGCAGACT ACGAAGCCTA TGTCAAGTGT CAAGATAAAG TGAGTCAGCT GTACATGAAT 
CCAAAGGCCT GGAACACAAT GGTACTCAAA AACATAGCTG CCTCGGGGAA ATTCTCCAGT 
GACCGAACAA TTAAAGAATA TGCCCAAAAC ATCTGGAACG TGGAACCTTC AGATCTAAAG 
ATTTCTCTAT CCAATGAATC TAACAAAGTC AATGGAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157412.1
CDS	81..2522
Translation	MAKPLTDQEKRRQISIRGIVGVENVAELKKSFNRHLHFTLVKDRNVATTRDYYFALAHTVRDHLVGRWIRTQQHYYDKCPKLGLDIEELEEIEEDAGLGNGGLGRLAACFLDSMATLGLAAYGYGIRYEYGIFNQKIRDGWQVEEADDWLRYGNPWEKSRPEFMLPVHFYGKVEHTNTGTKWIDTQVVLALPYDTPVPGYMNNTVNTMRLWSARAPNDFNLRDFNVGDYIQAVLDRNLAENISRVLYPNDNFFEGKELRLKQEYFVVAATLQDIIRRFKASKFGSTRGAGTVFDAFPDQVAIQLNDTHPALAIPELMRIFVDIEKLPWSKAWELTQKTFAYTNHTVLPEALERWPVDLVEKLLPRHLEIIYEINQKHLDRIVALFPKDVDRLRRMSLIEEEGSKRINMAHLCIVGSHAVNGVAKIHSDIVKTKVFKDFSELEPDKFQNKTNGITPRRWLLLCNPGLAELIAEKIGEDYVKDLSQLTKLHSFLGDDVFLRELAKVKQENKLKFSQFLETEYKVKINPSSMFDVQVKRIHEYKRQLLNCLHVITMYNRIKKDPKKLFVPRTVIIGGKAAPGYHMAKMIIKLITSVADVVNNDPMVGSKLKVIFLENYRVSLAEKVIPATDLSEQISTAGTEASGTGNMKFMLNGALTIGTMDGANVEMAEEAGEENLFIFGMRIDDVAALDKKGYEAKEYYEALPELKLVIDQIDNGFFSPKQPDLFKDIINMLFYHDRFKVFADYEAYVKCQDKVSQLYMNPKAWNTMVLKNIAASGKFSSDRTIKEYAQNIWNVEPSDLKISLSNESNKVNGN

Target ORF information:

RefSeq Version	NM_001163940.2
Organism	Homo sapiens(human)
Definition	Homo sapiens glycogen phosphorylase L (PYGL), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163940.2

ORF Insert Sequence:

ATGGCGAAGC CCCTGACGGA CCAGGAGAAG CGGCGGCAGA TCAGCATCCG CGGCATCGTG 
GGCGTGGAGA ACGTGGCAGA GCTGAAGAAG AGTTTCAACC GGCACCTGCA CTTCACGCTG 
GTCAAGGACC GCAACGTGGC CACCACCCGC GACTACTACT TCGCGCTGGC GCACACGGTG 
CGCGACCACC TGGTGGGGCG CTGGATCCGC ACGCAGCAGC ACTACTACGA CAAGTGCCCC 
AAGCTTGGAT TGGATATAGA AGAGTTAGAA GAAATTGAAG AAGATGCTGG ACTTGGCAAT 
GGTGGTCTTG GGAGACTTGC TGCCTGCTTC TTGGATTCCA TGGCAACCCT GGGACTTGCA 
GCCTATGGAT ACGGCATTCG GTATGAATAT GGGATTTTCA ATCAGAAGAT CCGAGATGGA 
TGGCAGGTAG AAGAAGCAGA TGATTGGCTC AGATATGGAA ACCCTTGGGA GAAGTCCCGC 
CCAGAATTCA TGCTGCCTGT GCACTTCTAT GGAAAAGTAG AACACACCAA CACCGGGACC 
AAGTGGATTG ACACTCAAGT GGTCCTGGCT CTGCCATATG ACACCCCCGT GCCCGGCTAC 
ATGAATAACA CTGTCAACAC CATGCGCCTC TGGTCTGCTC GGGCACCAAA TGACTTTAAC 
CTCAGAGACT TTAATGTTGG AGACTACATT CAGGCTGTGC TGGACCGAAA CCTGGCCGAG 
AACATCTCCC GGGTCCTCTA TCCCAATGAC AATTTTTTTG AAGGGAAGGA GCTAAGATTG 
AAGCAGGAAT ACTTTGTGGT GGCTGCAACC TTGCAAGATA TCATCCGCCG TTTCAAAGCC 
TCCAAGTTTG GCTCCACCCG TGGTGCAGGA ACTGTGTTTG ATGCCTTCCC GGATCAGGTG 
GCCATCCAGC TGAATGACAC TCACCCTGCA CTCGCGATCC CTGAGCTGAT GAGGATTTTT 
GTGGATATTG AAAAACTGCC CTGGTCCAAG GCATGGGAGC TCACCCAGAA GACCTTCGCC 
TACACCAACC ACACAGTGCT CCCGGAAGCC CTGGAGCGCT GGCCCGTGGA CCTGGTGGAG 
AAGCTGCTCC CTCGACATTT GGAAATCATT TATGAGATAA ATCAGAAGCA TTTAGATAGA 
ATTGTGGCCT TGTTTCCTAA AGATGTGGAC CGTCTGAGAA GGATGTCTCT GATAGAAGAG 
GAAGGAAGCA AAAGGATCAA CATGGCCCAT CTCTGCATTG TCGGTTCCCA TGCTGTGAAT 
GGCGTGGCTA AAATCCACTC AGACATCGTG AAGACTAAAG TATTCAAGGA CTTCAGTGAG 
CTAGAACCTG ACAAGTTTCA GAATAAAACC AATGGGATCA CTCCAAGGCG CTGGCTCCTA 
CTCTGCAACC CAGGACTTGC AGAGCTCATA GCAGAGAAAA TTGGAGAAGA CTATGTGAAA 
GACCTGAGCC AGCTGACGAA GCTCCACAGC TTCCTGGGTG ATGATGTCTT CCTCCGGGAA 
CTCGCCAAGG TGAAGCAGGA GAATAAGCTG AAGTTTTCTC AGTTCCTGGA GACGGAGTAC 
AAAGTGAAGA TCAACCCATC CTCCATGTTT GATGTCCAGG TGAAGAGGAT ACATGAGTAC 
AAGCGACAGC TCTTGAACTG TCTGCATGTG ATCACGATGT ACAACCGCAT TAAGAAAGAC 
CCTAAGAAGT TATTCGTGCC AAGGACAGTT ATCATTGGTG GTAAAGCTGC CCCAGGATAT 
CACATGGCCA AAATGATCAT AAAGCTGATC ACTTCAGTGG CAGATGTGGT GAACAATGAC 
CCTATGGTTG GAAGCAAGTT GAAAGTCATC TTCTTGGAGA ACTACAGAGT ATCTCTTGCT 
GAAAAAGTCA TTCCAGCCAC AGATCTGTCA GAGCAGATTT CCACTGCAGG CACCGAAGCC 
TCGGGGACAG GCAATATGAA GTTCATGCTA AATGGGGCCC TAACTATCGG GACCATGGAT 
GGGGCCAATG TGGAAATGGC AGAAGAAGCT GGGGAAGAGA ACCTGTTCAT CTTTGGCATG 
AGGATAGATG ATGTGGCTGC TTTGGACAAG AAAGGGTACG AGGCAAAAGA ATACTATGAG 
GCACTTCCAG AGCTGAAGCT GGTCATTGAT CAAATTGACA ATGGCTTTTT TTCTCCCAAG 
CAGCCTGACC TCTTCAAAGA TATCATCAAC ATGCTATTTT ATCATGACAG GTTTAAAGTC 
TTTGCAGACT ACGAAGCCTA TGTCAAGTGT CAAGATAAAG TGAGTCAGCT GTACATGAAT 
CCAAAGGCCT GGAACACAAT GGTACTCAAA AACATAGCTG CCTCGGGGAA ATTCTCCAGT 
GACCGAACAA TTAAAGAATA TGCCCAAAAC ATCTGGAACG TGGAACCTTC AGATCTAAAG 
ATTTCTCTAT CCAATGAATC TAACAAAGTC AATGGAAATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kov?cs IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barab?si AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

Genome-wide association study of atypical psychosis.
American journal of medical genetics. Part B, Neuropsychiatric genetics : the official publication of the International Society of Psychiatric Genetics162B(7)679-86(2013 Oct)
Kanazawa T,Ikeda M,Glatt SJ,Tsutsumi A,Kikuyama H,Kawamura Y,Nishida N,Miyagawa T,Hashimoto R,Takeda M,Sasaki T,Tokunaga K,Koh J,Iwata N,Yoneda H

Genome-wide association study identifies germline polymorphisms associated with relapse of childhood acute lymphoblastic leukemia.
Blood120(20)4197-204(2012 Nov)
Yang JJ,Cheng C,Devidas M,Cao X,Campana D,Yang W,Fan Y,Neale G,Cox N,Scheet P,Borowitz MJ,Winick NJ,Martin PL,Bowman WP,Camitta B,Reaman GH,Carroll WL,Willman CL,Hunger SP,Evans WE,Pui CH,Loh M,Relling MV

Acetylation negatively regulates glycogen phosphorylase by recruiting protein phosphatase 1.
Cell metabolism15(1)75-87(2012 Jan)
Zhang T,Wang S,Lin Y,Xu W,Ye D,Xiong Y,Zhao S,Guan KL

Investigation of genetic susceptibility factors for human longevity - a targeted nonsynonymous SNP study.
Mutation research694(1-2)13-9(2010 Dec)
Flachsbart F,Franke A,Kleindorp R,Caliebe A,Blanch? H,Schreiber S,Nebel A

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PYGL cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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