NFE4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NFE4
Entrez Gene ID	58160
Full Name	nuclear factor, erythroid 4
Synonyms	NF-E4
General protein information	Preferred Names nuclear factor, erythroid 4 Names transcription factor NF-E4 fetal globin activator NF-E4 gamma-globin gene activator
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 7 Map Location: 7q22.1
Summary	The erythroid-specific protein encoded by this gene, and the ubiquitous transcription factor CP2, form the stage selector protein (SSP) complex, which is involved in preferential expression of the gamma-globin genes in fetal erythroid cells. Alternate use of an in-frame upstream non-AUG (CUG) translation initiation codon, and a downstream AUG codon, results in two isoforms. While the long isoform (22 kDa) acts as an activator, the short isoform (14 kDa) has been shown to repress gamma-globin gene expression. This gene is located in an intron of the FBXL13 gene on the opposite strand. [provided by RefSeq, Jul 2008].
Disorder MIM:	612133

mRNA and Protein(s)

mRNA	Protein	Name
NM_001085386.1	NP_001078855.1	transcription factor NF-E4
NM_001085386.2	NP_001078855.1	transcription factor NF-E4

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Repression of human gamma-globin gene expression by a short isoform of the NF-E4 protein is associated with loss of NF-E2 and RNA polymerase II recruitment to the promoter.
Zhao Q, Zhou W, Rank G, Sutton R, Wang X, Cumming H, Cerruti L, Cunningham JM, Jane SM
Blood107(5)2138-45(2006 Mar)

Site-specific acetylation of the fetal globin activator NF-E4 prevents its ubiquitination and regulates its interaction with the histone deacetylase, HDAC1.
Zhao Q, Cumming H, Cerruti L, Cunningham JM, Jane SM
The Journal of biological chemistry279(40)41477-86(2004 Oct)

The role of p22 NF-E4 in human globin gene switching.
Zhou W, Zhao Q, Sutton R, Cumming H, Wang X, Cerruti L, Hall M, Wu R, Cunningham JM, Jane SM
The Journal of biological chemistry279(25)26227-32(2004 Jun)

Induction of human fetal globin gene expression by a novel erythroid factor, NF-E4.
Zhou W, Clouston DR, Wang X, Cerruti L, Cunningham JM, Jane SM
Molecular and cellular biology20(20)7662-72(2000 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

stabilization of NF-E4 by acetylation is PCAF-dependent; acetylation of Lys(43) also reduces the interaction between NF-E4 and HDAC1, potentially maximizing the activating ability of the factor
Title: Site-specific acetylation of the fetal globin activator NF-E4 prevents its ubiquitination and regulates its interaction with the histone deacetylase, HDAC1.

findings indicate that p22 NF-E4 is capable of influencing human globin gene expression in vivo but is incapable of overriding the intrinsic mechanisms governing gamma-gene silencing in this context
Title: The role of p22 NF-E4 in human globin gene switching.

Cloning and characterization of NF-E4 as a component of the stage selector protein complex, and demonstration of non-AUG translation initiation codon usage.
Title: Induction of human fetal globin gene expression by a novel erythroid factor, NF-E4.

The following NFE4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NFE4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30579	NM_001085386.2 Latest version!	Homo sapiens nuclear factor, erythroid 4 (NFE4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu30579	NM_001085386.1	Homo sapiens nuclear factor, erythroid 4 (NFE4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NFE4 ( NM_001085386.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001078855.1 Homo sapiens nuclear factor, erythroid 4 (NFE4), mRNA.

Price & Availability↑

CloneID	OHu30579
Clone ID Related Accession (Same CDS sequence)	NM_001085386.1 , NM_001085386.2
Accession Version	NM_001085386.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-29
Organism	Homo sapiens(human)
Product	transcription factor NF-E4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY258907.1, AC073127.11 and BC036938.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2159764, SAMN01820687 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 16263792, 11003662 ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

CTGCCTCGTG TTGTCTGTTG GCACACTCTC AAGAGTTTGA ACGGATACAA GAATCTTTCA 
TCTGGTGCCG AAACCCGGGA GGGGCTCCGG TCTTCGTCCC CCGTGGACCT ACCCCTCCGC 
CCCAGAAAGC AGGCCACAGC AGCCGGACAA AGGAAGCTCC TCAGCCTCCA GTTGCTTCTC 
TGTGCATGCA CATCAGTCAC TGATCTCACC TACTGGGGCC CTGCAGGCCA TGGGGCCACA 
GCTCCACACA GAAGCCTCCT AGCAATCCAC CTCCACCTGG TGCCTGCTTC AAGTGCGGCA 
ATGAAGGCCA CTGGCCCACA CAATGCCCAA ACCCAGGTAA ACCCACGAGG CCATGCCCCC 
TCTGCGGAGG ACCCCACTGG AAGTTGGACT GTGAGCGGCC CCTGCAAGGA CCACCCCCAT 
CCCTTCCTGA GCCAATCAAA CCCTCCTACT CGGATCTCGT CAGCCTTGCC GCTGAAGACT 
GATAGTGCCT TGGAACAGAC ACCCCAGCAA CTACCATCGC TTCATCTGAG CCAAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078855.1
CDS	121..660
Translation	LPRVVCWHTLKSLNGYKNLSSGAETREGLRSSSPVDLPLRPRKQATAAGQRKLLSLQLLLCACTSVTDLTYWGPAGHGATAPHRSLLAIHLHLVPASSAAMKATGPHNAQTQVNPRGHAPSAEDPTGSWTVSGPCKDHPHPFLSQSNPPTRISSALPLKTDSALEQTPQQLPSLHLSQG

Target ORF information:

RefSeq Version	NM_001085386.1
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor, erythroid 4 (NFE4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085386.1

ORF Insert Sequence:

CTGCCTCGTG TTGTCTGTTG GCACACTCTC AAGAGTTTGA ACGGATACAA GAATCTTTCA 
TCTGGTGCCG AAACCCGGGA GGGGCTCCGG TCTTCGTCCC CCGTGGACCT ACCCCTCCGC 
CCCAGAAAGC AGGCCACAGC AGCCGGACAA AGGAAGCTCC TCAGCCTCCA GTTGCTTCTC 
TGTGCATGCA CATCAGTCAC TGATCTCACC TACTGGGGCC CTGCAGGCCA TGGGGCCACA 
GCTCCACACA GAAGCCTCCT AGCAATCCAC CTCCACCTGG TGCCTGCTTC AAGTGCGGCA 
ATGAAGGCCA CTGGCCCACA CAATGCCCAA ACCCAGGTAA ACCCACGAGG CCATGCCCCC 
TCTGCGGAGG ACCCCACTGG AAGTTGGACT GTGAGCGGCC CCTGCAAGGA CCACCCCCAT 
CCCTTCCTGA GCCAATCAAA CCCTCCTACT CGGATCTCGT CAGCCTTGCC GCTGAAGACT 
GATAGTGCCT TGGAACAGAC ACCCCAGCAA CTACCATCGC TTCATCTGAG CCAAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NFE4 ( NM_001085386.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001078855.1 Homo sapiens nuclear factor, erythroid 4 (NFE4), mRNA.

Price & Availability↑

CloneID	OHu30579
Clone ID Related Accession (Same CDS sequence)	NM_001085386.1 , NM_001085386.2
Accession Version	NM_001085386.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-30
Organism	Homo sapiens(human)
Product	transcription factor NF-E4
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC073127.11, AY258907.1 and BC036938.1. On Nov 22, 2018 this sequence version replaced NM_001085386.1. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA2159764, SAMN01820687 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## non-AUG initiation codon :: PMID: 16263792, 11003662 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

CTGCCTCGTG TTGTCTGTTG GCACACTCTC AAGAGTTTGA ACGGATACAA GAATCTTTCA 
TCTGGTGCCG AAACCCGGGA GGGGCTCCGG TCTTCGTCCC CCGTGGACCT ACCCCTCCGC 
CCCAGAAAGC AGGCCACAGC AGCCGGACAA AGGAAGCTCC TCAGCCTCCA GTTGCTTCTC 
TGTGCATGCA CATCAGTCAC TGATCTCACC TACTGGGGCC CTGCAGGCCA TGGGGCCACA 
GCTCCACACA GAAGCCTCCT AGCAATCCAC CTCCACCTGG TGCCTGCTTC AAGTGCGGCA 
ATGAAGGCCA CTGGCCCACA CAATGCCCAA ACCCAGGTAA ACCCACGAGG CCATGCCCCC 
TCTGCGGAGG ACCCCACTGG AAGTTGGACT GTGAGCGGCC CCTGCAAGGA CCACCCCCAT 
CCCTTCCTGA GCCAATCAAA CCCTCCTACT CGGATCTCGT CAGCCTTGCC GCTGAAGACT 
GATAGTGCCT TGGAACAGAC ACCCCAGCAA CTACCATCGC TTCATCTGAG CCAAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001078855.1
CDS	128..667
Translation	LPRVVCWHTLKSLNGYKNLSSGAETREGLRSSSPVDLPLRPRKQATAAGQRKLLSLQLLLCACTSVTDLTYWGPAGHGATAPHRSLLAIHLHLVPASSAAMKATGPHNAQTQVNPRGHAPSAEDPTGSWTVSGPCKDHPHPFLSQSNPPTRISSALPLKTDSALEQTPQQLPSLHLSQG

Target ORF information:

RefSeq Version	NM_001085386.2
Organism	Homo sapiens(human)
Definition	Homo sapiens nuclear factor, erythroid 4 (NFE4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001085386.2

ORF Insert Sequence:

CTGCCTCGTG TTGTCTGTTG GCACACTCTC AAGAGTTTGA ACGGATACAA GAATCTTTCA 
TCTGGTGCCG AAACCCGGGA GGGGCTCCGG TCTTCGTCCC CCGTGGACCT ACCCCTCCGC 
CCCAGAAAGC AGGCCACAGC AGCCGGACAA AGGAAGCTCC TCAGCCTCCA GTTGCTTCTC 
TGTGCATGCA CATCAGTCAC TGATCTCACC TACTGGGGCC CTGCAGGCCA TGGGGCCACA 
GCTCCACACA GAAGCCTCCT AGCAATCCAC CTCCACCTGG TGCCTGCTTC AAGTGCGGCA 
ATGAAGGCCA CTGGCCCACA CAATGCCCAA ACCCAGGTAA ACCCACGAGG CCATGCCCCC 
TCTGCGGAGG ACCCCACTGG AAGTTGGACT GTGAGCGGCC CCTGCAAGGA CCACCCCCAT 
CCCTTCCTGA GCCAATCAAA CCCTCCTACT CGGATCTCGT CAGCCTTGCC GCTGAAGACT 
GATAGTGCCT TGGAACAGAC ACCCCAGCAA CTACCATCGC TTCATCTGAG CCAAGGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Repression of human gamma-globin gene expression by a short isoform of the NF-E4 protein is associated with loss of NF-E2 and RNA polymerase II recruitment to the promoter.
Blood107(5)2138-45(2006 Mar)
Zhao Q,Zhou W,Rank G,Sutton R,Wang X,Cumming H,Cerruti L,Cunningham JM,Jane SM

Site-specific acetylation of the fetal globin activator NF-E4 prevents its ubiquitination and regulates its interaction with the histone deacetylase, HDAC1.
The Journal of biological chemistry279(40)41477-86(2004 Oct)
Zhao Q,Cumming H,Cerruti L,Cunningham JM,Jane SM

The role of p22 NF-E4 in human globin gene switching.
The Journal of biological chemistry279(25)26227-32(2004 Jun)
Zhou W,Zhao Q,Sutton R,Cumming H,Wang X,Cerruti L,Hall M,Wu R,Cunningham JM,Jane SM

Induction of human fetal globin gene expression by a novel erythroid factor, NF-E4.
Molecular and cellular biology20(20)7662-72(2000 Oct)
Zhou W,Clouston DR,Wang X,Cerruti L,Cunningham JM,Jane SM

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NFE4 cDNA ORF clone, Homo sapiens(human)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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