POLD4 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	POLD4
Entrez Gene ID	57804
Full Name	DNA polymerase delta 4, accessory subunit
Synonyms	POLDS,p12
General protein information	Preferred Names DNA polymerase delta 4, accessory subunit Names DNA polymerase delta subunit 4 DNA polymerase delta smallest subunit p12 polymerase (DNA) delta 4, accessory subunit polymerase (DNA-directed), delta 4, accessory subunit
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 11 Map Location: 11q13.2
Summary	This gene encodes the smallest subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. The encoded protein enhances the activity of DNA polymerase delta and plays a role in fork repair and stabilization through interactions with the DNA helicase Bloom syndrome protein. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Mar 2012].
Disorder MIM:	611525

mRNA and Protein(s)

mRNA	Protein	Name
NM_021173.4	NP_066996.3	DNA polymerase delta subunit 4 isoform 1
NM_001256870.1	NP_001243799.1	DNA polymerase delta subunit 4 isoform 2
NM_021173.5	NP_066996.3	DNA polymerase delta subunit 4 isoform 1

Pathways from BioSystems

Homologies

	POLD4	XP_001107074.1
Rattus norvegicus (Norway rat)	Pold4	NP_001013213.1
Pan troglodytes (chimpanzee)	POLD4	XP_001173013.1
Canis lupus familiaris (dog)	POLD4	XP_854661.1
Homo sapiens (human)	POLD4	NP_066996.3
Mus musculus (house mouse)	Pold4	NP_081472.1
Bos taurus (cattle)	POLD4	NP_001069949.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

The tail that wags the dog: p12, the smallest subunit of DNA polymerase ?, is degraded by ubiquitin ligases in response to DNA damage and during cell cycle progression.
Lee MY, Zhang S, Lin SH, Wang X, Darzynkiewicz Z, Zhang Z, Lee EY
Cell cycle (Georgetown, Tex.)13(1)23-31(2014)

Proteolysis of the human DNA polymerase delta smallest subunit p12 by ?-calpain in calcium-triggered apoptotic HeLa cells.
Fan X, Zhang Q, You C, Qian Y, Gao J, Liu P, Chen H, Song H, Chen Y, Chen K, Zhou Y
PloS one9(4)e93642(2014)

Dynamics of enzymatic interactions during short flap human Okazaki fragment processing by two forms of human DNA polymerase ?.
Lin SH, Wang X, Zhang S, Zhang Z, Lee EY, Lee MY
DNA repair12(11)922-35(2013 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

A parallel study of Pol delta4 and Pol delta3 in Okazaki fragment processing provides evidence for a role of Pol delta3 in DNA replication
Title: The tail that wags the dog: p12, the smallest subunit of DNA polymerase ?, is degraded by ubiquitin ligases in response to DNA damage and during cell cycle progression.

the p12/Pol delta is a target as a nuclear substrate of mu-calpain in a calcium-triggered apoptosis and appears to be a potential marker in the study of the chemotherapy of cancer therapies.
Title: Proteolysis of the human DNA polymerase delta smallest subunit p12 by ?-calpain in calcium-triggered apoptotic HeLa cells.

Findings provide evidence for the novel concept that Pol delta3 has a role in lagging strand synthesis, and that both forms of Pol delta3 and 4 may participate in DNA replication in higher eukaryotic cells.
Title: Dynamics of enzymatic interactions during short flap human Okazaki fragment processing by two forms of human DNA polymerase ?.

Data indicate that CRL4(Cdt2) regulates the degradation of the p12 subunit of Pol delta4.
Title: A novel function of CRL4(Cdt2): regulation of the subunit structure of DNA polymerase ? in response to DNA damage and during the S phase.

ubiquitination of p12 through CRL4(Cdt2) and subsequent degradation form one mechanism by which a cell responds to DNA damage to inhibit fork progression.
Title: Degradation of p12 subunit by CRL4Cdt2 E3 ligase inhibits fork progression after DNA damage.

The following POLD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the POLD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11766	NM_021173.5 Latest version!	Homo sapiens DNA polymerase delta 4, accessory subunit (POLD4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
OHu10809	NM_001256870.1 Latest version!	Homo sapiens DNA polymerase delta 4, accessory subunit (POLD4), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$69.30 $99.00
View Old Accession Versions >>
OHu11766	NM_021173.4	Homo sapiens DNA polymerase delta 4, accessory subunit (POLD4), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

POLD4 ( NM_021173.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_066996.3 Homo sapiens DNA polymerase delta 4, accessory subunit (POLD4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11766
Clone ID Related Accession (Same CDS sequence)	NM_021173.4 , NM_021173.5
Accession Version	NM_021173.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-02
Organism	Homo sapiens(human)
Product	DNA polymerase delta subunit 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM697399.1, BC070250.1, AF179890.1 and AP003419.3. On Mar 2, 2012 this sequence version replaced NM_021173.3. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070250.1, SRR1163657.529253.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCGGA AGCGGCTCAT CACTGATTCC TACCCGGTTG TGAAGAGGAG GGAGGGGCCC 
GCTGGGCACA GCAAGGGGGA GCTGGCACCC GAGCTAGGGG AGGAGCCCCA GCCCCGCGAC 
GAGGAGGAAG CGGAGCTGGA GCTGCTGAGG CAGTTTGACC TGGCCTGGCA GTACGGGCCC 
TGCACCGGGA TCACACGGCT GCAGCGCTGG TGTCGGGCCA AGCAGATGGG CTTGGAGCCT 
CCCCCAGAGG TGTGGCAGGT GCTGAAGACC CACCCCGGAG ACCCCCGCTT CCAGTGCAGT 
CTCTGGCATC TCTATCCCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066996.3
CDS	198..521
Translation	MGRKRLITDSYPVVKRREGPAGHSKGELAPELGEEPQPRDEEEAELELLRQFDLAWQYGPCTGITRLQRWCRAKQMGLEPPPEVWQVLKTHPGDPRFQCSLWHLYPL

Target ORF information:

RefSeq Version	NM_021173.4
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase delta 4, accessory subunit (POLD4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021173.4

ORF Insert Sequence:

ATGGGCCGGA AGCGGCTCAT CACTGATTCC TACCCGGTTG TGAAGAGGAG GGAGGGGCCC 
GCTGGGCACA GCAAGGGGGA GCTGGCACCC GAGCTAGGGG AGGAGCCCCA GCCCCGCGAC 
GAGGAGGAAG CGGAGCTGGA GCTGCTGAGG CAGTTTGACC TGGCCTGGCA GTACGGGCCC 
TGCACCGGGA TCACACGGCT GCAGCGCTGG TGTCGGGCCA AGCAGATGGG CTTGGAGCCT 
CCCCCAGAGG TGTGGCAGGT GCTGAAGACC CACCCCGGAG ACCCCCGCTT CCAGTGCAGT 
CTCTGGCATC TCTATCCCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLD4 ( NM_021173.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_066996.3 Homo sapiens DNA polymerase delta 4, accessory subunit (POLD4), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu11766
Clone ID Related Accession (Same CDS sequence)	NM_021173.4 , NM_021173.5
Accession Version	NM_021173.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 324bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	DNA polymerase delta subunit 4 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC070250.1, AF179890.1 and AP003419.3. On Nov 23, 2018 this sequence version replaced NM_021173.4. Transcript Variant: This variant (1) encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC070250.1, SRR3476690.800184.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000312419.8/ ENSP00000311368.3 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGGCCGGA AGCGGCTCAT CACTGATTCC TACCCGGTTG TGAAGAGGAG GGAGGGGCCC 
GCTGGGCACA GCAAGGGGGA GCTGGCACCC GAGCTAGGGG AGGAGCCCCA GCCCCGCGAC 
GAGGAGGAAG CGGAGCTGGA GCTGCTGAGG CAGTTTGACC TGGCCTGGCA GTACGGGCCC 
TGCACCGGGA TCACACGGCT GCAGCGCTGG TGTCGGGCCA AGCAGATGGG CTTGGAGCCT 
CCCCCAGAGG TGTGGCAGGT GCTGAAGACC CACCCCGGAG ACCCCCGCTT CCAGTGCAGT 
CTCTGGCATC TCTATCCCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_066996.3
CDS	148..471
Translation	MGRKRLITDSYPVVKRREGPAGHSKGELAPELGEEPQPRDEEEAELELLRQFDLAWQYGPCTGITRLQRWCRAKQMGLEPPPEVWQVLKTHPGDPRFQCSLWHLYPL

Target ORF information:

RefSeq Version	NM_021173.5
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase delta 4, accessory subunit (POLD4), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_021173.5

ORF Insert Sequence:

ATGGGCCGGA AGCGGCTCAT CACTGATTCC TACCCGGTTG TGAAGAGGAG GGAGGGGCCC 
GCTGGGCACA GCAAGGGGGA GCTGGCACCC GAGCTAGGGG AGGAGCCCCA GCCCCGCGAC 
GAGGAGGAAG CGGAGCTGGA GCTGCTGAGG CAGTTTGACC TGGCCTGGCA GTACGGGCCC 
TGCACCGGGA TCACACGGCT GCAGCGCTGG TGTCGGGCCA AGCAGATGGG CTTGGAGCCT 
CCCCCAGAGG TGTGGCAGGT GCTGAAGACC CACCCCGGAG ACCCCCGCTT CCAGTGCAGT 
CTCTGGCATC TCTATCCCCT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

POLD4 ( NM_001256870.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001243799.1 Homo sapiens DNA polymerase delta 4, accessory subunit (POLD4), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu10809
Clone ID Related Accession (Same CDS sequence)	NM_001256870.1
Accession Version	NM_001256870.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 240bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	DNA polymerase delta subunit 4 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BM697399.1, BG576821.1, BG403692.1 and AP003419.3. Transcript Variant: This variant (2) lacks an exon in the coding region, which results in a frameshift, compared to variant 1. The encoded isoform (2) is shorter and has a distinct C-terminus, compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BG403692.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCCGGA AGCGGCTCAT CACTGATTCC TACCCGGTTG TGAAGAGGAG GGAGGGGCCC 
GCTGGGCACA GCAAGGGGGA GCTGGCACCC GAGCTAGGGG AGGAGCCCCA GCCCCGCGAC 
GAGGAGGAAG CGGAGCTGGA GCTGCTGAGG CAGTTTGACC TGGCCTGGCA GTACGGGCCC 
TGCACCGTCT CTGGCATCTC TATCCCCTAT GAGGCACCAC GTAAGACCTC CTGCCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001243799.1
CDS	198..437
Translation	MGRKRLITDSYPVVKRREGPAGHSKGELAPELGEEPQPRDEEEAELELLRQFDLAWQYGPCTVSGISIPYEAPRKTSCP

Target ORF information:

RefSeq Version	NM_001256870.1
Organism	Homo sapiens(human)
Definition	Homo sapiens DNA polymerase delta 4, accessory subunit (POLD4), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001256870.1

ORF Insert Sequence:

ATGGGCCGGA AGCGGCTCAT CACTGATTCC TACCCGGTTG TGAAGAGGAG GGAGGGGCCC 
GCTGGGCACA GCAAGGGGGA GCTGGCACCC GAGCTAGGGG AGGAGCCCCA GCCCCGCGAC 
GAGGAGGAAG CGGAGCTGGA GCTGCTGAGG CAGTTTGACC TGGCCTGGCA GTACGGGCCC 
TGCACCGTCT CTGGCATCTC TATCCCCTAT GAGGCACCAC GTAAGACCTC CTGCCCTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

The tail that wags the dog: p12, the smallest subunit of DNA polymerase ?, is degraded by ubiquitin ligases in response to DNA damage and during cell cycle progression.
Cell cycle (Georgetown, Tex.)13(1)23-31(2014)
Lee MY,Zhang S,Lin SH,Wang X,Darzynkiewicz Z,Zhang Z,Lee EY

Proteolysis of the human DNA polymerase delta smallest subunit p12 by ?-calpain in calcium-triggered apoptotic HeLa cells.
PloS one9(4)e93642(2014)
Fan X,Zhang Q,You C,Qian Y,Gao J,Liu P,Chen H,Song H,Chen Y,Chen K,Zhou Y

Dynamics of enzymatic interactions during short flap human Okazaki fragment processing by two forms of human DNA polymerase ?.
DNA repair12(11)922-35(2013 Nov)
Lin SH,Wang X,Zhang S,Zhang Z,Lee EY,Lee MY

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POLD4 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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