SPTBN4 cDNA ORF clone, Homo sapiens(human)

The following SPTBN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPTBN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu04416 NM_025213.3
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant sigma5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $482.30
$689.00
OHu19524 NM_020971.3
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant sigma1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu19524 XM_017027049.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu70939 XM_011527173.2
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu88601 XM_017027050.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu88602 XM_017027051.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1091.30
$1559.00
OHu88603 XM_017027052.1
Latest version!
Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $643.30
$919.00
View Old Accession Versions >>
OHu19524 NM_020971.2 Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant sigma1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $1301.30
$1859.00
OHu04416 NM_025213.2 Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant sigma5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $482.30
$689.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu04416
    Clone ID Related Accession (Same CDS sequence) NM_025213.3 , NM_025213.2
    Accession Version NM_025213.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-19
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 4 isoform sigma5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311856.1, DA514440.1 and AC020929.6. On May 31, 2019 this sequence version replaced NM_025213.2. Transcript Variant: This variant (sigma5) contains a distinct 5' and 3' UTR and lacks an in-frame portion of the 5' and 3' coding region, compared to variant sigma1. The resulting isoform (sigma5), also known as the 77 kDa isoform, has distinct and shorter N- and C-termini when compared to isoform sigma1. ##Evidence-Data-START## Transcript exon combination :: AF311856.1, SRR7410570.654901.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCTGATGG CGCGGGATGG CACGCGGGAG GACAACCACA AGCTGCATAA GAGATGGCTC 
    CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATCGAG
    CGGGAGGGCC AGCAACTGAT GCAGGAGAAG CCCGAACTGG CGGCCTCCGT GCGGAAGAAG
    CTGGGCGAGA TCCGCCAGTG CTGGGCGGAG CTGGAGAGCA CCACCCAGGC CAAGGCACGG
    CAGCTCTTTG AGGCCAGCAA AGCAGACCAG CTGGTGCAGA GCTTTGCTGA GCTGGACAAG
    AAGCTCCTTC ACATGGAGAG CCAGCTGCAA GACGTGGACC CTGGAGGAGA CCTGGCCACT
    GTCAACAGTC AGCTCAAGAA GCTGCAGTCC ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
    GAGGTGGGAG AGCTGCAGGC GCAGACGGCG GCGCTGCCGC TGGAGCCGGC GAGCAAGGAG
    CTGGTGGGTG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGTTG
    CAGGAGCGCC GCCGCTTGCT GCTGGCTTCC AAGGAGTTGC ACCAGGTGGC GCACGACCTG
    GACGACGAGC TGGCATGGGT TCAGGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC
    AACGGTTTGC AGGCGGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
    CAGGCGCATG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGCGCGCT GGCGTCGCTG
    CGCAGCCCGG AGGCAGAGGC AGTGCGCCGG GGCCTGGAGC AGCTGCAGAG CGCCTGGGCC
    GGACTGCGGG AGGCTGCCGA GCGACGGCAG CAGGTGCTGG ACGCCGCCTT CCAGGTGGAG
    CAGTACTACT TCGACGTGGC TGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
    ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAACACCTG
    CAGCTGGAGC AAGGCGTGGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
    CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
    GTGGACCGCC TGTACGTGGC GCTCAAGGAG CTGGGTGAGG AGCGCCGGGT GGCTCTGGAA
    CAGCAGTACT GGCTGTACCA GCTCAGCCGC CAGGTGAGCG AGCTTGAGCA CTGGATTGCC
    GAGAAGGAGG TGGTGGCTGG CTCACCCGAG CTCGGCCAGG ACTTTGAGCA TGTCTCGGTG
    CTGCAGGAGA AATTCTCAGA GTTTGCCAGC GAGACAGGTA TGGCAGGGCG GGAACGGCTG
    GCAGCTGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCATACAGC AGCGGCCACC
    ATGGCCGAGT GGAAGGACGG ACTGAACGAG GCCTGGGCTG AGCTGCTGGA GCTCATGGGC
    ACACGGGCCC AGCTGCTGGC CGCCTCTCGG GAGCTTCATA AGTTCTTCAG TGACGCCCGA
    GAGCTTCAGG GACAGATTGA GGAGAAGCGG AGGCGGCTGC CCCGCCTGAC CACCCCGCCT
    GAGCCGAGAC CCAGTGCCAG TTCCATGCAG CGGACCCTGA GAGCCTTTGA GCATGACCTG
    CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CGGCCCAGCT GCGGACGGTG
    TATGCGGGTG AACATGCCGA GGCCATCGCT AGCCGGGAGC AGGAGGTGCT GCAGGGTTGG
    AAAGAGCTGC TGTCAGCCTG TGAGGATGCC CGCCTGCATG TCAGCTCCAC AGCCGACGCC
    CTGCGCTTCC ACAGCCAAGT CCGCGACCTG CTCTCCTGGA TGGATGGCAT CGCCAGCCAG
    ATTGGGGCAG CCGACAAGCC CAGGTGCCCC TCATCCCTCC TCGGGCTTCC TGCCTCCCCC
    TGGTGGCCTA CCCCAGCCAC CCCCAGCCCA TTGACAGCCC CCTTCTCAAT GGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079489.2
    CDS125..2161
    Translation

    Target ORF information:

    RefSeq Version NM_025213.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant sigma5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025213.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCTGATGG CGCGGGATGG CACGCGGGAG GACAACCACA AGCTGCATAA GAGATGGCTC 
    CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATCGAG
    CGGGAGGGCC AGCAACTGAT GCAGGAGAAG CCCGAACTGG CGGCCTCCGT GCGGAAGAAG
    CTGGGCGAGA TCCGCCAGTG CTGGGCGGAG CTGGAGAGCA CCACCCAGGC CAAGGCACGG
    CAGCTCTTTG AGGCCAGCAA AGCAGACCAG CTGGTGCAGA GCTTTGCTGA GCTGGACAAG
    AAGCTCCTTC ACATGGAGAG CCAGCTGCAA GACGTGGACC CTGGAGGAGA CCTGGCCACT
    GTCAACAGTC AGCTCAAGAA GCTGCAGTCC ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
    GAGGTGGGAG AGCTGCAGGC GCAGACGGCG GCGCTGCCGC TGGAGCCGGC GAGCAAGGAG
    CTGGTGGGTG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGTTG
    CAGGAGCGCC GCCGCTTGCT GCTGGCTTCC AAGGAGTTGC ACCAGGTGGC GCACGACCTG
    GACGACGAGC TGGCATGGGT TCAGGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC
    AACGGTTTGC AGGCGGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
    CAGGCGCATG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGCGCGCT GGCGTCGCTG
    CGCAGCCCGG AGGCAGAGGC AGTGCGCCGG GGCCTGGAGC AGCTGCAGAG CGCCTGGGCC
    GGACTGCGGG AGGCTGCCGA GCGACGGCAG CAGGTGCTGG ACGCCGCCTT CCAGGTGGAG
    CAGTACTACT TCGACGTGGC TGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
    ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAACACCTG
    CAGCTGGAGC AAGGCGTGGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
    CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
    GTGGACCGCC TGTACGTGGC GCTCAAGGAG CTGGGTGAGG AGCGCCGGGT GGCTCTGGAA
    CAGCAGTACT GGCTGTACCA GCTCAGCCGC CAGGTGAGCG AGCTTGAGCA CTGGATTGCC
    GAGAAGGAGG TGGTGGCTGG CTCACCCGAG CTCGGCCAGG ACTTTGAGCA TGTCTCGGTG
    CTGCAGGAGA AATTCTCAGA GTTTGCCAGC GAGACAGGTA TGGCAGGGCG GGAACGGCTG
    GCAGCTGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCATACAGC AGCGGCCACC
    ATGGCCGAGT GGAAGGACGG ACTGAACGAG GCCTGGGCTG AGCTGCTGGA GCTCATGGGC
    ACACGGGCCC AGCTGCTGGC CGCCTCTCGG GAGCTTCATA AGTTCTTCAG TGACGCCCGA
    GAGCTTCAGG GACAGATTGA GGAGAAGCGG AGGCGGCTGC CCCGCCTGAC CACCCCGCCT
    GAGCCGAGAC CCAGTGCCAG TTCCATGCAG CGGACCCTGA GAGCCTTTGA GCATGACCTG
    CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CGGCCCAGCT GCGGACGGTG
    TATGCGGGTG AACATGCCGA GGCCATCGCT AGCCGGGAGC AGGAGGTGCT GCAGGGTTGG
    AAAGAGCTGC TGTCAGCCTG TGAGGATGCC CGCCTGCATG TCAGCTCCAC AGCCGACGCC
    CTGCGCTTCC ACAGCCAAGT CCGCGACCTG CTCTCCTGGA TGGATGGCAT CGCCAGCCAG
    ATTGGGGCAG CCGACAAGCC CAGGTGCCCC TCATCCCTCC TCGGGCTTCC TGCCTCCCCC
    TGGTGGCCTA CCCCAGCCAC CCCCAGCCCA TTGACAGCCC CCTTCTCAAT GGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu04416
    Clone ID Related Accession (Same CDS sequence) NM_025213.3 , NM_025213.2
    Accession Version NM_025213.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2037bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 4 isoform sigma5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311856.1 and DA514440.1. On Sep 30, 2006 this sequence version replaced NM_025213.1. Transcript Variant: This variant (sigma5) contains a distinct 5' and 3' UTR and lacks an in-frame portion of the 5' and 3' coding region, compared to variant sigma1. The resulting isoform (sigma5), also known as the 77 kDa isoform, has distinct and shorter N- and C-termini when compared to isoform sigma1. ##Evidence-Data-START## Transcript exon combination :: AF311856.1, SRR1660807.197546.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCTGATGG CGCGGGATGG CACGCGGGAG GACAACCACA AGCTGCATAA GAGATGGCTC 
    CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATCGAG
    CGGGAGGGCC AGCAACTGAT GCAGGAGAAG CCCGAACTGG CGGCCTCCGT GCGGAAGAAG
    CTGGGCGAGA TCCGCCAGTG CTGGGCGGAG CTGGAGAGCA CCACCCAGGC CAAGGCACGG
    CAGCTCTTTG AGGCCAGCAA AGCAGACCAG CTGGTGCAGA GCTTTGCTGA GCTGGACAAG
    AAGCTCCTTC ACATGGAGAG CCAGCTGCAA GACGTGGACC CTGGAGGAGA CCTGGCCACT
    GTCAACAGTC AGCTCAAGAA GCTGCAGTCC ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
    GAGGTGGGAG AGCTGCAGGC GCAGACGGCG GCGCTGCCGC TGGAGCCGGC GAGCAAGGAG
    CTGGTGGGTG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGTTG
    CAGGAGCGCC GCCGCTTGCT GCTGGCTTCC AAGGAGTTGC ACCAGGTGGC GCACGACCTG
    GACGACGAGC TGGCATGGGT TCAGGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC
    AACGGTTTGC AGGCGGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
    CAGGCGCATG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGCGCGCT GGCGTCGCTG
    CGCAGCCCGG AGGCAGAGGC AGTGCGCCGG GGCCTGGAGC AGCTGCAGAG CGCCTGGGCC
    GGACTGCGGG AGGCTGCCGA GCGACGGCAG CAGGTGCTGG ACGCCGCCTT CCAGGTGGAG
    CAGTACTACT TCGACGTGGC TGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
    ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAACACCTG
    CAGCTGGAGC AAGGCGTGGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
    CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
    GTGGACCGCC TGTACGTGGC GCTCAAGGAG CTGGGTGAGG AGCGCCGGGT GGCTCTGGAA
    CAGCAGTACT GGCTGTACCA GCTCAGCCGC CAGGTGAGCG AGCTTGAGCA CTGGATTGCC
    GAGAAGGAGG TGGTGGCTGG CTCACCCGAG CTCGGCCAGG ACTTTGAGCA TGTCTCGGTG
    CTGCAGGAGA AATTCTCAGA GTTTGCCAGC GAGACAGGTA TGGCAGGGCG GGAACGGCTG
    GCAGCTGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCATACAGC AGCGGCCACC
    ATGGCCGAGT GGAAGGACGG ACTGAACGAG GCCTGGGCTG AGCTGCTGGA GCTCATGGGC
    ACACGGGCCC AGCTGCTGGC CGCCTCTCGG GAGCTTCATA AGTTCTTCAG TGACGCCCGA
    GAGCTTCAGG GACAGATTGA GGAGAAGCGG AGGCGGCTGC CCCGCCTGAC CACCCCGCCT
    GAGCCGAGAC CCAGTGCCAG TTCCATGCAG CGGACCCTGA GAGCCTTTGA GCATGACCTG
    CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CGGCCCAGCT GCGGACGGTG
    TATGCGGGTG AACATGCCGA GGCCATCGCT AGCCGGGAGC AGGAGGTGCT GCAGGGTTGG
    AAAGAGCTGC TGTCAGCCTG TGAGGATGCC CGCCTGCATG TCAGCTCCAC AGCCGACGCC
    CTGCGCTTCC ACAGCCAAGT CCGCGACCTG CTCTCCTGGA TGGATGGCAT CGCCAGCCAG
    ATTGGGGCAG CCGACAAGCC CAGGTGCCCC TCATCCCTCC TCGGGCTTCC TGCCTCCCCC
    TGGTGGCCTA CCCCAGCCAC CCCCAGCCCA TTGACAGCCC CCTTCTCAAT GGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_079489.2
    CDS128..2164
    Translation

    Target ORF information:

    RefSeq Version NM_025213.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant sigma5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_025213.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGCTGATGG CGCGGGATGG CACGCGGGAG GACAACCACA AGCTGCATAA GAGATGGCTC 
    CGGCACCAGG CATTCATGGC CGAGCTGGCT CAGAATAAGG AGTGGCTGGA GAAGATCGAG
    CGGGAGGGCC AGCAACTGAT GCAGGAGAAG CCCGAACTGG CGGCCTCCGT GCGGAAGAAG
    CTGGGCGAGA TCCGCCAGTG CTGGGCGGAG CTGGAGAGCA CCACCCAGGC CAAGGCACGG
    CAGCTCTTTG AGGCCAGCAA AGCAGACCAG CTGGTGCAGA GCTTTGCTGA GCTGGACAAG
    AAGCTCCTTC ACATGGAGAG CCAGCTGCAA GACGTGGACC CTGGAGGAGA CCTGGCCACT
    GTCAACAGTC AGCTCAAGAA GCTGCAGTCC ATGGAGTCGC AGGTGGAGGA GTGGTACCGC
    GAGGTGGGAG AGCTGCAGGC GCAGACGGCG GCGCTGCCGC TGGAGCCGGC GAGCAAGGAG
    CTGGTGGGTG AGCGGCAGAA CGCGGTGGGC GAGCGCCTGG TGCGCCTGCT CGAGCCGTTG
    CAGGAGCGCC GCCGCTTGCT GCTGGCTTCC AAGGAGTTGC ACCAGGTGGC GCACGACCTG
    GACGACGAGC TGGCATGGGT TCAGGAGCGG CTGCCACTGG CCATGCAGAC AGAGCGAGGC
    AACGGTTTGC AGGCGGTCCA GCAGCACATC AAAAAGAACC AGGGCCTGCG GCGGGAGATC
    CAGGCGCATG GGCCGCGCCT GGAGGAGGTG CTGGAGCGCG CGGGCGCGCT GGCGTCGCTG
    CGCAGCCCGG AGGCAGAGGC AGTGCGCCGG GGCCTGGAGC AGCTGCAGAG CGCCTGGGCC
    GGACTGCGGG AGGCTGCCGA GCGACGGCAG CAGGTGCTGG ACGCCGCCTT CCAGGTGGAG
    CAGTACTACT TCGACGTGGC TGAGGTGGAG GCGTGGCTGG GCGAGCAGGA GCTGCTCATG
    ATGAGTGAGG ACAAGGGCAA GGACGAACAG AGCACCCTGC AGCTGCTCAA GAAACACCTG
    CAGCTGGAGC AAGGCGTGGA GAACTACGAG GAAAGCATCG CGCAGCTGTC GCGCCAGTGC
    CGGGCGCTGC TGGAGATGGG GCACCCGGAC AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG
    GTGGACCGCC TGTACGTGGC GCTCAAGGAG CTGGGTGAGG AGCGCCGGGT GGCTCTGGAA
    CAGCAGTACT GGCTGTACCA GCTCAGCCGC CAGGTGAGCG AGCTTGAGCA CTGGATTGCC
    GAGAAGGAGG TGGTGGCTGG CTCACCCGAG CTCGGCCAGG ACTTTGAGCA TGTCTCGGTG
    CTGCAGGAGA AATTCTCAGA GTTTGCCAGC GAGACAGGTA TGGCAGGGCG GGAACGGCTG
    GCAGCTGTGA ACCAGATGGT GGATGAGCTG ATCGAGTGTG GCCATACAGC AGCGGCCACC
    ATGGCCGAGT GGAAGGACGG ACTGAACGAG GCCTGGGCTG AGCTGCTGGA GCTCATGGGC
    ACACGGGCCC AGCTGCTGGC CGCCTCTCGG GAGCTTCATA AGTTCTTCAG TGACGCCCGA
    GAGCTTCAGG GACAGATTGA GGAGAAGCGG AGGCGGCTGC CCCGCCTGAC CACCCCGCCT
    GAGCCGAGAC CCAGTGCCAG TTCCATGCAG CGGACCCTGA GAGCCTTTGA GCATGACCTG
    CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG CAGGAGGGGG CGGCCCAGCT GCGGACGGTG
    TATGCGGGTG AACATGCCGA GGCCATCGCT AGCCGGGAGC AGGAGGTGCT GCAGGGTTGG
    AAAGAGCTGC TGTCAGCCTG TGAGGATGCC CGCCTGCATG TCAGCTCCAC AGCCGACGCC
    CTGCGCTTCC ACAGCCAAGT CCGCGACCTG CTCTCCTGGA TGGATGGCAT CGCCAGCCAG
    ATTGGGGCAG CCGACAAGCC CAGGTGCCCC TCATCCCTCC TCGGGCTTCC TGCCTCCCCC
    TGGTGGCCTA CCCCAGCCAC CCCCAGCCCA TTGACAGCCC CCTTCTCAAT GGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19524
    Clone ID Related Accession (Same CDS sequence) XM_017027049.1 , NM_020971.2 , NM_020971.3
    Accession Version NM_020971.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 4 isoform sigma1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AF311855.1, AC020929.6, AB046862.1 and AI962525.1. This sequence is a reference standard in the RefSeqGene project. On Sep 30, 2006 this sequence version replaced NM_020971.1. Transcript Variant: This variant (sigma1) represents the longer transcript and encodes the longer isoform (sigma1), also known as the 289 kDa isoform. ##Evidence-Data-START## Transcript exon combination :: AF311855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2145245 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
    CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
    ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
    GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
    CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
    GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
    GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
    GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
    ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
    TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
    AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
    CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
    CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
    GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
    TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
    TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
    CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
    TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
    GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
    CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
    AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
    CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
    AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
    CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
    TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
    TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
    ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
    TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
    CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
    GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
    TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
    CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
    GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
    CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
    GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
    CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
    TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
    CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
    GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
    GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
    TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
    GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
    GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
    CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
    CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
    GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
    CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
    AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
    TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
    GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
    GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
    CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
    CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
    CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
    GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
    CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
    AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
    CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
    GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
    TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
    TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
    GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
    CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
    GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
    ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
    CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
    ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
    AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
    GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
    GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
    GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
    GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
    GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
    GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
    GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
    CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
    GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
    ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
    CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
    CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
    AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
    TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
    GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
    AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
    TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
    CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
    GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
    CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
    CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
    CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
    GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
    GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
    AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
    ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
    GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
    CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
    CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
    ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
    ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
    ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
    TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
    GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
    TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
    GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
    GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
    GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
    AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
    GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
    GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
    CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
    AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
    CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
    CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
    GCTGGGCCGT CGCTGCCTCA GCCACGCGAG CTTCCCCCAG GTCGCCTGCC CAACGGGCTT
    GAGCTGCCCG AGCGGACACC TCGGCCGGAC CGGCCCCGGG CGCGGGACCG GCCCAAGCCG
    CGACGGCGGC CGCGGCCCAG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
    GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCGCCAC CGCCCACTCA CACAGTGCAG
    CACGAGGGCT TCCTACTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
    TCGTGGGTGA GCCTGTACTG TGTGCTTAGT AAGGGGGAAC TGGGCTTCTA CAAGGACTCC
    AAGGGCCCGG CATCCGGGAG CACACACGGT GGGGAACCGC TGCTCAGCCT GCACAAGGCC
    ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
    GATGGCAGTG AGTTTTTGCT CCAGGCAAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
    GCTGTAGCTT CCTCGGTGGC GGAACACGCA GAGATCGCCC GCTGGGGCCA GACACTACCC
    ACTACTTCAT CCACAGATGA GGGCAACCCT AAGAGGGAAG GCGGAGATCG CAGGGCCAGC
    GGGCGCAGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_066022.2
    CDS103..7797
    Translation

    Target ORF information:

    RefSeq Version NM_020971.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant sigma1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020971.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
    CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
    ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
    GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
    CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
    GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
    GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
    GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
    ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
    TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
    AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
    CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
    CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
    GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
    TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
    TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
    CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
    TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
    GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
    CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
    AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
    CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
    AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
    CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
    TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
    TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
    ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
    TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
    CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
    GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
    TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
    CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
    GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
    CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
    GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
    CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
    TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
    CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
    GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
    GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
    TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
    GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
    GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
    CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
    CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
    GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
    CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
    AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
    TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
    GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
    GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
    CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
    CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
    CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
    GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
    CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
    AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
    CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
    GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
    TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
    TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
    GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
    CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
    GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
    ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
    CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
    ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
    AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
    GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
    GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
    GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
    GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
    GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
    GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
    GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
    CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
    GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
    ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
    CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
    CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
    AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
    TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
    GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
    AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
    TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
    CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
    GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
    CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
    CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
    CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
    GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
    GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
    AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
    ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
    GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
    CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
    CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
    ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
    ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
    ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
    TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
    GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
    TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
    GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
    GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
    GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
    AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
    GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
    GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
    CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
    AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
    CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
    CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
    GCTGGGCCGT CGCTGCCTCA GCCACGCGAG CTTCCCCCAG GTCGCCTGCC CAACGGGCTT
    GAGCTGCCCG AGCGGACACC TCGGCCGGAC CGGCCCCGGG CGCGGGACCG GCCCAAGCCG
    CGACGGCGGC CGCGGCCCAG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
    GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCGCCAC CGCCCACTCA CACAGTGCAG
    CACGAGGGCT TCCTACTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
    TCGTGGGTGA GCCTGTACTG TGTGCTTAGT AAGGGGGAAC TGGGCTTCTA CAAGGACTCC
    AAGGGCCCGG CATCCGGGAG CACACACGGT GGGGAACCGC TGCTCAGCCT GCACAAGGCC
    ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
    GATGGCAGTG AGTTTTTGCT CCAGGCAAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
    GCTGTAGCTT CCTCGGTGGC GGAACACGCA GAGATCGCCC GCTGGGGCCA GACACTACCC
    ACTACTTCAT CCACAGATGA GGGCAACCCT AAGAGGGAAG GCGGAGATCG CAGGGCCAGC
    GGGCGCAGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19524
    Clone ID Related Accession (Same CDS sequence) XM_017027049.1 , NM_020971.2 , NM_020971.3
    Accession Version NM_020971.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 4 isoform sigma1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC020929.6, AF311855.1, AB046862.1 and AI962525.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_020971.2. Transcript Variant: This variant (sigma1) represents the longer transcript and encodes the longer isoform (sigma1), also known as the 289 kDa isoform. ##Evidence-Data-START## Transcript exon combination :: AF311855.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2145245, SAMEA2145743 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000598249.6/ ENSP00000469242.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
    CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
    ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
    GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
    CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
    GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
    GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
    GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
    ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
    TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
    AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
    CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
    CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
    GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
    TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
    TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
    CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
    TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
    GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
    CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
    AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
    CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
    AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
    CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
    TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
    TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
    ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
    TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
    CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
    GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
    TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
    CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
    GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
    CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
    GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
    CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
    TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
    CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
    GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
    GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
    TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
    GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
    GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
    CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
    CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
    GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
    CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
    AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
    TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
    GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
    GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
    CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
    CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
    CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
    GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
    CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
    AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
    CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
    GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
    TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
    TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
    GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
    CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
    GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
    ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
    CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
    ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
    AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
    GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
    GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
    GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
    GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
    GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
    GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
    GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
    CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
    GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
    ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
    CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
    CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
    AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
    TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
    GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
    AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
    TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
    CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
    GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
    CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
    CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
    CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
    GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
    GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
    AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
    ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
    GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
    CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
    CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
    ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
    ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
    ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
    TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
    GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
    TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
    GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
    GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
    GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
    AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
    GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
    GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
    CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
    AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
    CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
    CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
    GCTGGGCCGT CGCTGCCTCA GCCACGCGAG CTTCCCCCAG GTCGCCTGCC CAACGGGCTT
    GAGCTGCCCG AGCGGACACC TCGGCCGGAC CGGCCCCGGG CGCGGGACCG GCCCAAGCCG
    CGACGGCGGC CGCGGCCCAG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
    GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCGCCAC CGCCCACTCA CACAGTGCAG
    CACGAGGGCT TCCTACTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
    TCGTGGGTGA GCCTGTACTG TGTGCTTAGT AAGGGGGAAC TGGGCTTCTA CAAGGACTCC
    AAGGGCCCGG CATCCGGGAG CACACACGGT GGGGAACCGC TGCTCAGCCT GCACAAGGCC
    ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
    GATGGCAGTG AGTTTTTGCT CCAGGCAAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
    GCTGTAGCTT CCTCGGTGGC GGAACACGCA GAGATCGCCC GCTGGGGCCA GACACTACCC
    ACTACTTCAT CCACAGATGA GGGCAACCCT AAGAGGGAAG GCGGAGATCG CAGGGCCAGC
    GGGCGCAGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_066022.2
    CDS321..8015
    Translation

    Target ORF information:

    RefSeq Version NM_020971.3
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant sigma1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020971.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
    CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
    ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
    GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
    CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
    GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
    GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
    GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
    ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
    TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
    AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
    CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
    CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
    GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
    TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
    TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
    CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
    TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
    GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
    CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
    AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
    CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
    AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
    CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
    TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
    TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
    ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
    TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
    CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
    GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
    TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
    CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
    GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
    CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
    GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
    CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
    TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
    CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
    GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
    GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
    TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
    GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
    GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
    CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
    CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
    GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
    CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
    AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
    TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
    GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
    GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
    CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
    CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
    CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
    GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
    CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
    AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
    CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
    GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
    TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
    TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
    GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
    CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
    GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
    ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
    CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
    ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
    AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
    GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
    GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
    GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
    GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
    GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
    GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
    GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
    CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
    GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
    ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
    CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
    CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
    AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
    TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
    GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
    AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
    TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
    CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
    GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
    CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
    CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
    CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
    GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
    GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
    AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
    ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
    GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
    CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
    CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
    ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
    ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
    ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
    TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
    GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
    TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
    GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
    GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
    GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
    AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
    GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
    GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
    CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
    AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
    CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
    CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
    GCTGGGCCGT CGCTGCCTCA GCCACGCGAG CTTCCCCCAG GTCGCCTGCC CAACGGGCTT
    GAGCTGCCCG AGCGGACACC TCGGCCGGAC CGGCCCCGGG CGCGGGACCG GCCCAAGCCG
    CGACGGCGGC CGCGGCCCAG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
    GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCGCCAC CGCCCACTCA CACAGTGCAG
    CACGAGGGCT TCCTACTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
    TCGTGGGTGA GCCTGTACTG TGTGCTTAGT AAGGGGGAAC TGGGCTTCTA CAAGGACTCC
    AAGGGCCCGG CATCCGGGAG CACACACGGT GGGGAACCGC TGCTCAGCCT GCACAAGGCC
    ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
    GATGGCAGTG AGTTTTTGCT CCAGGCAAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
    GCTGTAGCTT CCTCGGTGGC GGAACACGCA GAGATCGCCC GCTGGGGCCA GACACTACCC
    ACTACTTCAT CCACAGATGA GGGCAACCCT AAGAGGGAAG GCGGAGATCG CAGGGCCAGC
    GGGCGCAGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19524
    Clone ID Related Accession (Same CDS sequence) XM_017027049.1 , NM_020971.2 , NM_020971.3
    Accession Version XM_017027049.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7695bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
    CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
    ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
    GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
    CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
    GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
    GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
    GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
    ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
    TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
    AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
    CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
    CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
    GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
    TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
    TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
    CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
    TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
    GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
    CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
    AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
    CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
    AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
    CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
    TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
    TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
    ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
    TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
    CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
    GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
    TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
    CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
    GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
    CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
    GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
    CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
    TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
    CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
    GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
    GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
    TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
    GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
    GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
    CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
    CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
    GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
    CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
    AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
    TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
    GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
    GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
    CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
    CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
    CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
    GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
    CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
    AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
    CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
    GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
    TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
    TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
    GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
    CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
    GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
    ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
    CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
    ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
    AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
    GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
    GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
    GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
    GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
    GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
    GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
    GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
    CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
    GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
    ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
    CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
    CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
    AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
    TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
    GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
    AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
    TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
    CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
    GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
    CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
    CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
    CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
    GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
    GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
    AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
    ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
    GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
    CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
    CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
    ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
    ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
    ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
    TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
    GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
    TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
    GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
    GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
    GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
    AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
    GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
    GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
    CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
    AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
    CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
    CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
    GCTGGGCCGT CGCTGCCTCA GCCACGCGAG CTTCCCCCAG GTCGCCTGCC CAACGGGCTT
    GAGCTGCCCG AGCGGACACC TCGGCCGGAC CGGCCCCGGG CGCGGGACCG GCCCAAGCCG
    CGACGGCGGC CGCGGCCCAG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
    GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCGCCAC CGCCCACTCA CACAGTGCAG
    CACGAGGGCT TCCTACTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
    TCGTGGGTGA GCCTGTACTG TGTGCTTAGT AAGGGGGAAC TGGGCTTCTA CAAGGACTCC
    AAGGGCCCGG CATCCGGGAG CACACACGGT GGGGAACCGC TGCTCAGCCT GCACAAGGCC
    ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
    GATGGCAGTG AGTTTTTGCT CCAGGCAAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
    GCTGTAGCTT CCTCGGTGGC GGAACACGCA GAGATCGCCC GCTGGGGCCA GACACTACCC
    ACTACTTCAT CCACAGATGA GGGCAACCCT AAGAGGGAAG GCGGAGATCG CAGGGCCAGC
    GGGCGCAGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016882538.1
    CDS356..8050
    Translation

    Target ORF information:

    RefSeq Version XM_017027049.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027049.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
    CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
    ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
    GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
    CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
    GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
    GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
    GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
    ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
    TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
    AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
    CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
    CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
    GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
    TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
    TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
    CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
    TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
    GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
    CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
    AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
    CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
    AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
    CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
    TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
    TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
    ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
    TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
    CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
    GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
    TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
    CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
    GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
    CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
    GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
    CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
    TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
    CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
    GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
    GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
    TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
    GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
    GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
    CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
    CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
    GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
    CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
    AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
    TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
    GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
    GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
    CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
    CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
    CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
    GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
    CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
    AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
    CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
    GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
    TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
    TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
    GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
    CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
    GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
    ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
    CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
    ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
    AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
    GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
    GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
    GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
    GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
    GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
    GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
    GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
    CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
    GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
    ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
    CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
    CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
    AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
    TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
    GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
    AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
    TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
    CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
    GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
    CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
    CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
    CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
    GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
    GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
    AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
    ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
    GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
    CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
    CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
    ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
    ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
    ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
    TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
    GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
    TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
    GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
    GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
    GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
    AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
    GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
    GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
    CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
    AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
    CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
    CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
    GCTGGGCCGT CGCTGCCTCA GCCACGCGAG CTTCCCCCAG GTCGCCTGCC CAACGGGCTT
    GAGCTGCCCG AGCGGACACC TCGGCCGGAC CGGCCCCGGG CGCGGGACCG GCCCAAGCCG
    CGACGGCGGC CGCGGCCCAG AGAGGGTGGT GAGGGCGGGG GAAGCCGGCG CTCGCGCTCC
    GCCCCGGCCC AGGGCGGCTC CGCCCCCGCG CCTCCGCCAC CGCCCACTCA CACAGTGCAG
    CACGAGGGCT TCCTACTGCG CAAGCGCGAG CTCGACGCTA ACCGCAAGTC GTCCAACCGG
    TCGTGGGTGA GCCTGTACTG TGTGCTTAGT AAGGGGGAAC TGGGCTTCTA CAAGGACTCC
    AAGGGCCCGG CATCCGGGAG CACACACGGT GGGGAACCGC TGCTCAGCCT GCACAAGGCC
    ACCAGCGAGG TGGCTAGTGA CTACAAGAAA AAGAAGCATG TCTTCAAGCT CCAGACCCAG
    GATGGCAGTG AGTTTTTGCT CCAGGCAAAA GATGAGGAGG AGATGAACGG CTGGCTGGAG
    GCTGTAGCTT CCTCGGTGGC GGAACACGCA GAGATCGCCC GCTGGGGCCA GACACTACCC
    ACTACTTCAT CCACAGATGA GGGCAACCCT AAGAGGGAAG GCGGAGATCG CAGGGCCAGC
    GGGCGCAGGA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70939
    Clone ID Related Accession (Same CDS sequence) XM_011527173.2
    Accession Version XM_011527173.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7056bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011527173.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
    CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
    ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
    GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
    CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
    GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
    GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
    GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
    ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
    TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
    AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
    CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
    CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
    GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
    TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
    TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
    CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
    TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
    GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
    CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
    AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
    CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
    AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
    CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
    TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
    TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
    ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
    TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
    CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
    GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
    TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
    CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
    GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
    CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
    GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
    CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
    TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
    CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
    GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
    GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
    TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
    GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
    GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
    CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
    CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
    GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
    CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
    AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
    TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
    GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
    GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
    CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
    CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
    CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
    GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
    CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
    AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
    CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
    GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
    TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
    TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
    GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
    CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
    GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
    ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
    CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
    ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
    AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
    GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
    GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
    GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
    GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
    GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
    GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
    GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
    CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
    GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
    ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
    CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
    CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
    AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
    TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
    GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
    AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
    TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
    CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
    GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
    CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
    CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
    CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
    GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
    GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
    AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
    ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
    GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
    CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
    CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
    ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
    ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
    ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
    TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
    GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
    TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
    GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
    GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
    GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
    AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
    GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
    GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
    CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
    AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
    CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
    CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
    GCTGGGGTCG TGGGTGAGCC TGTACTGTGT GCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011525475.1
    CDS169..7224
    Translation

    Target ORF information:

    RefSeq Version XM_011527173.2
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011527173.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
    CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
    ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
    GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
    CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
    GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
    GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
    GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
    ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
    TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
    AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
    CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
    CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
    GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
    TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
    TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
    CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
    TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
    GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
    CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
    AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
    CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
    AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
    CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
    TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
    TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
    ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
    TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
    CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
    GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
    TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
    CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
    GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
    CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
    GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
    CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
    TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
    CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
    GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
    GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
    TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
    GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
    GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
    CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
    CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
    GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
    CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
    AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
    TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
    GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
    GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
    CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
    CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
    CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
    GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
    CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
    AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
    CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
    GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
    TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
    TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
    GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
    CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
    GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
    ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
    CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
    ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
    AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
    GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
    GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
    GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
    GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
    GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
    GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
    GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
    CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
    GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
    ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
    CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
    CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
    AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
    TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
    GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
    AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
    TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
    CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
    GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
    CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
    CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
    CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
    GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
    GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
    AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
    ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
    GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
    CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
    CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
    ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
    ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
    ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
    TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
    GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
    TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
    GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
    GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
    GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGATCG AGAAAATCAA AGCGGAACAG
    AGCAAGCAGC CGCCTACCCC ACTGCTGGGG CGCAAGTTCT TTGGGGACCC CACGGAACTG
    GCGGCCAAGG CGGCGCCCCT GCTGCGGCCA GGGGGCTATG AAAGGGGCTT GGAGCCCCTG
    GCCCGCCGAG CCTCGGACAC GCTCTCGGCC GAGGTGCGGA CTCGGGTGGG GTATGTGCGC
    CAGGAGCTCA AGCCCGAGCG CCTCCAGCCG CGCATTGACC GGCTGCCGGA GATCCCGGGG
    AGGGTGGAGC CCGCGGCCCT GCCGGCCGCA CCAGAGGACG CGGCGGAGAC CCCCGCGACC
    CCCGCGGCGG CGGAGCAGGT GCGGCCACGA CCGGAGCGCC AGGAGTCAGC TGATCGCGCG
    GAGGAGCTGC CCAGGAGGCG GCGGCCTGAG CGGCAAGAGT CAGTCGATCA ATCCGAGGAG
    GCTGCGCGGA GGCGGCGGCC GGAGCGGCAG GAGTCAGCGG AGCACGAGGC GGCACACAGC
    CTTACCCTGG GCCGCTATGA GCAGATGGAG CGGCGGCGCG AGCGGCGTGA GCGGCGCTTG
    GAGCGGCAGG AGTCCAGCGA ACAGGAGATG CCCATCAGAG GAGACCTGGT CAAGGGGAAG
    GCCACCCTGG CTGACATTGT GGAACAGCTG CAGGAGAAAG AGGCAGGCCC AGGGCTGCCT
    GCTGGGGTCG TGGGTGAGCC TGTACTGTGT GCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88601
    Clone ID Related Accession (Same CDS sequence) XM_017027050.1
    Accession Version XM_017027050.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 7353bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCGGATCC ACTCACTGGA GAACGTGGAC AAGGCGCTGC AGTTTCTGAA GGAGCAGCGC 
    GTGCACCTGG AGAACGTGGG TTCGCATGAC ATCGTGGATG GGAATCACCG GCTGACGCTG
    GGGCTGGTCT GGACCATCAT CCTGCGCTTC CAGATTCAAG TCATCAAAAT TGAGACTGAG
    GACAACAGAG AGACACGCTC AGCCAAGGAT GCTCTGCTCT TGTGGTGTCA GATGAAGACA
    GCTGGTTACC CTGAGGTAAA CATCCAGAAT TTCACCACCA GCTGGCGGGA TGGCTTGGCC
    TTCAATGCCC TCATTCACCG GCACAGGCCT GATCTCGTGG ACTTCAGCAA ACTCACCAAG
    TCCAATGCCA ACTACAACCT GCAGAGAGCC TTCCGCACAG CTGAGCAGCA CCTGGGGCTG
    GCGCGGCTGC TGGATCCTGA AGATGTGAAC ATGGAGGCTC CAGATGAGAA GTCCATCATC
    ACCTACGTGG TCTCTTTCTA CCACTATTTC TCCAAGATGA AGGCTCTGGC TGTGGAGGGG
    AAGCGTATCG GGAAGGTCTT GGACCAGGTA TTGGAGGTGG GGAAGATCAT AGAACGCTAC
    GAGGAGCTGG CGGCTGAGCT GCTGGCCTGG ATCCACCGCA CCGTGGGCCT CATCAGCAAT
    CAGAAATTTG CCAACTCCTT AAGTGGGGTG CAGCAGCAAC TCCAGGCTTT CACGGCCTAT
    TGCACGCTGG AGAAGCCTGT CAAGTTCCAG GAGAAGGGGA ACCTAGAGGT GCTGCTCTTC
    AGCATCCAGA GCAAACTGCG TGCCTGCAAC CGTCGCCTCT TTGTGCCTCG GGAGGGCTGT
    GGCATCTGGG ATATTGACAA GGCATGGGGT GAGCTGGAGA AGGCTGAGCA TGAGCGGGAG
    GCTGCCCTAC GGGCTGAGCT GATTCGGCAG GAGAAGCTGG AACTACTGGC ACAGAGGTTT
    GACCACAAGG TGGCTATGAG GGAGAGCTGG CTGAATGAGA ACCAGCGTCT GGTCTCCCAG
    GACAACTTTG GGTATGAGCT GCCCGCAGTG GAGGCAGCCA TGAAGAAACA CGAAGCGATC
    GAGGCAGACA TTGCGGCCTA CGAGGAGCGG GTGCAGGGTG TGGCGGAGCT GGCCCAGGCA
    TTGGCAGCCG AAGGCTACTA CGATATCCGG CGGGTGGCAG CCCAGCGTGA CAGCGTCCTG
    CGCCAGTGGG CCCTGCTAAC TGGGCTTGTG GGTGCCCGGC GGACACGACT TGAGCAGAAC
    CTTGCCCTGC AGAAGGTCTT CCAGGAGATG GTGTACATGG TGGACTGGAT GGAGGAGATG
    CAGGCTCAGC TGCTGTCCCG GGAGTGTGGG CAGCACCTGG TGGAGGCAGA CGACCTGTTG
    CAGAAGCATG GACTGCTGGA GGGAGACATT GCCGCCCAGA GCGAGCGGGT GGAGGCTCTC
    AATGCCGCTG CCCTGCGCTT CTCCCAGCTG CAGGGCTACC AGCCCTGCGA CCCGCAGGTC
    ATCTGCAACC GCGTGAACCA CGTGCACGGC TGCCTGGCGG AGCTGCAGGA GCAGGCAGCG
    CGGCGACGCG CGGAGCTGGA GGCTTCGCGG AGCCTGTGGG CGCTGCTGCA GGAGCTGGAG
    GAGGCCGAGA GCTGGGCGCG CGACAAGGAG CGTCTCCTGG AGGCTGCGGG CGGCGGCGGT
    GCGGCGGGCG CAGCGGGCGC AGCGGGAACA GCGGGCGGCG CGCATGACCT GTCCAGCACA
    GCGCGCCTCC TGGCCCAGCA CAAGATCCTG CAGGGCGAGC TGGGCGGGCG GCGAGCGTTG
    CTGCAGCAGG CCCTGCGGTG TGGCGAGGAG CTGGTTGCGG CCGGCGGTGC CGTCGGCCCG
    GGAGCAGACA CCGTGCACCT GGTAGGCCTG GCGGAGCGCG CGGCGAGCGC CCGGCGCCGC
    TGGCAGAGGC TGGAAGAGGC GGCGGCGCGG CGAGAGCGGC GGCTGCAGGA GGCGCGGGCG
    CTGCACCAGT TCGGCGCTGA CCTCGACGGG CTGCTGGACT GGCTTCGCGA CGCTTACCGC
    CTGGCAGCCG CCGGTGACTT CGGCCACGAC GAAGCTTCCA GCCGCCGCCT GGCGCGCCAG
    CACCGCGCGC TCACCGGGGA GGTGGAGGCA CATCGCGGGC CCGTGAGCGG CCTGCGGCGC
    CAGCTGGCGA CACTCGGGGG TGCCAGTGGC GCAGGGCCAC TGGTGGTGGC GCTGCAGGTG
    CGCGTGGTGG AAGCAGAGCA GTTGTTCGCT GAGGTGACCG AAGTGGCGGC GCTGAGGCGC
    CAGTGGCTGC GGGACGCGCT CGCTGTCTAC CGCATGTTTG GCGAGGTGCA CGCGTGTGAG
    CTGTGGATCG GCGAGAAGGA GCAATGGCTG CTCTCCATGC GTGTGCCGGA TTCACTCGAC
    GACGTCGAGG TGGTGCAGCA CCGATTCGAG AGCCTGGACC AAGAGATGAA CAGCCTGATG
    GGCCGCGTTC TGGACGTGAA CCACACAGTC CAGGAGCTGG TGGAAGGAGG CCACCCCAGT
    TCAGATGAGG TGCGTTCCTG CCAGGACCAC CTCAACAGCA GGTGGAACCG CATCGTGGAG
    CTAGTGGAAC AGCGCAAAGA GGAAATGAGC GCGGTGCTGC TGGTGGAGAA CCACGTGCTG
    GAGGTGGCCG AGGTGCGCGC CCAGGTGCGT GAGAAGCGGA GAGCTGTGGA GAGCGCGCCC
    CGGGCCGGCG GCGCCCTGCA GTGGCGTCTT AGCGGCCTAG AGGCCGCTCT GCAGGCGCTG
    GAGCCGCGCC AGGCGGCCCT TCTGGAGGAG GCAGCCCTGC TGGCTGAGCG CTTCCCGGCG
    CAGGCGGCGC GGCTGCACCA GGGCGCGGAG GAGCTGGGCG CCGAGTGGGG CGCGCTAGCT
    AGCGCGGCTC AGGCCTGCGG CGAGGCGGTG GCGGCAGCAG GGCGCCTGCA GCGCTTCCTA
    CATGACCTCG ACGCTTTCCT GGACTGGCTC GTGCGCGCCC AGGAGGCGGC GGGCGGCAGC
    GAGGGGCCCC TGCCCAACAG CCTAGAAGAG GCGGACGCGC TGCTGGCGCG CCACGCTGCG
    CTCAAGGAGG AGGTGGACCA GCGCGAGGAA GACTATGCTC GCATCGTGGC GGCCAGCGAG
    GCGCTGCTGG CCGCCGACGG CGCAGAGCTG GGCCCGGGCC TGGCACTAGA CGAGTGGCTG
    CCACACCTCG AACTTGGCTG GCATAAACTG CTCGGCTTGT GGGAGGCGCG CAGGGAGGCG
    CTGGTCCAGG CGCACATCTA CCAGCTCTTC CTGCGGGATC TACGCCAGGC GCTCGTGGTG
    CTGCGTAACC AGGAGATGGC GCTGTCTGGT GCGGAGCTCC CGGGCACAGT GGAATCGGTG
    GAGGAGGCCT TGAAACAGCA CCGTGACTTT CTCACCACCA TGGAGCTGAG CCAGCAAAAG
    ATGCAGGTGG CCGTGCAGGC TGCAGAGGGC CTGCTGAGGC AGGGCAACAT CTACGGGGAG
    CAGGCTCAGG AGGCTGTGAC CCGGCTGCTG GAGAAGAACC AAGAAAACCA GTTACGGGCC
    CAGCAATGGA TGCAAAAGCT ACATGACCAA CTTGAGCTGC AGCACTTCCT CCGAGACTGC
    CACGAGCTGG ATGGCTGGAT CCATGAGAAG ATGCTGATGG CGCGGGATGG CACGCGGGAG
    GACAACCACA AGCTGCATAA GAGATGGCTC CGGCACCAGG CATTCATGGC CGAGCTGGCT
    CAGAATAAGG AGTGGCTGGA GAAGATCGAG CGGGAGGGCC AGCAACTGAT GCAGGAGAAG
    CCCGAACTGG CGGCCTCCGT GCGGAAGAAG CTGGGCGAGA TCCGCCAGTG CTGGGCGGAG
    CTGGAGAGCA CCACCCAGGC CAAGGCACGG CAGCTCTTTG AGGCCAGCAA AGCAGACCAG
    CTGGTGCAGA GCTTTGCTGA GCTGGACAAG AAGCTCCTTC ACATGGAGAG CCAGCTGCAA
    GACGTGGACC CTGGAGGAGA CCTGGCCACT GTCAACAGTC AGCTCAAGAA GCTGCAGTCC
    ATGGAGTCGC AGGTGGAGGA GTGGTACCGC GAGGTGGGAG AGCTGCAGGC GCAGACGGCG
    GCGCTGCCGC TGGAGCCGGC GAGCAAGGAG CTGGTGGGTG AGCGGCAGAA CGCGGTGGGC
    GAGCGCCTGG TGCGCCTGCT CGAGCCGTTG CAGGAGCGCC GCCGCTTGCT GCTGGCTTCC
    AAGGAGTTGC ACCAGGTGGC GCACGACCTG GACGACGAGC TGGCATGGGT TCAGGAGCGG
    CTGCCACTGG CCATGCAGAC AGAGCGAGGC AACGGTTTGC AGGCGGTCCA GCAGCACATC
    AAAAAGAACC AGGGCCTGCG GCGGGAGATC CAGGCGCATG GGCCGCGCCT GGAGGAGGTG
    CTGGAGCGCG CGGGCGCGCT GGCGTCGCTG CGCAGCCCGG AGGCAGAGGC AGTGCGCCGG
    GGCCTGGAGC AGCTGCAGAG CGCCTGGGCC GGACTGCGGG AGGCTGCCGA GCGACGGCAG
    CAGGTGCTGG ACGCCGCCTT CCAGGTGGAG CAGTACTACT TCGACGTGGC TGAGGTGGAG
    GCGTGGCTGG GCGAGCAGGA GCTGCTCATG ATGAGTGAGG ACAAGGGCAA GGACGAACAG
    AGCACCCTGC AGCTGCTCAA GAAACACCTG CAGCTGGAGC AAGGCGTGGA GAACTACGAG
    GAAAGCATCG CGCAGCTGTC GCGCCAGTGC CGGGCGCTGC TGGAGATGGG GCACCCGGAC
    AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG GTGGACCGCC TGTACGTGGC GCTCAAGGAG
    CTGGGTGAGG AGCGCCGGGT GGCTCTGGAA CAGCAGTACT GGCTGTACCA GCTCAGCCGC
    CAGGTGAGCG AGCTTGAGCA CTGGATTGCC GAGAAGGAGG TGGTGGCTGG CTCACCCGAG
    CTCGGCCAGG ACTTTGAGCA TGTCTCGGTG CTGCAGGAGA AATTCTCAGA GTTTGCCAGC
    GAGACAGGTA TGGCAGGGCG GGAACGGCTG GCAGCTGTGA ACCAGATGGT GGATGAGCTG
    ATCGAGTGTG GCCATACAGC AGCGGCCACC ATGGCCGAGT GGAAGGACGG ACTGAACGAG
    GCCTGGGCTG AGCTGCTGGA GCTCATGGGC ACACGGGCCC AGCTGCTGGC CGCCTCTCGG
    GAGCTTCATA AGTTCTTCAG TGACGCCCGA GAGCTTCAGG GACAGATTGA GGAGAAGCGG
    AGGCGGCTGC CCCGCCTGAC CACCCCGCCT GAGCCGAGAC CCAGTGCCAG TTCCATGCAG
    CGGACCCTGA GAGCCTTTGA GCATGACCTG CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG
    CAGGAGGGGG CGGCCCAGCT GCGGACGGTG TATGCGGGTG AACATGCCGA GGCCATCGCT
    AGCCGGGAGC AGGAGGTGCT GCAGGGTTGG AAAGAGCTGC TGTCAGCCTG TGAGGATGCC
    CGCCTGCATG TCAGCTCCAC AGCCGACGCC CTGCGCTTCC ACAGCCAAGT CCGCGACCTG
    CTCTCCTGGA TGGATGGCAT CGCCAGCCAG ATTGGGGCAG CCGACAAGCC CAGGGACGTG
    TCATCAGTGG AGGTGCTCAT GAACTACCAC CAGGGCCTGA AGACTGAGCT GGAGGCGCGG
    GTGCCTGAGC TGACCACCTG CCAGGAGCTG GGGCGATCTC TGCTGCTCAA CAAAAGTGCC
    ATGGCTGATG AGATCCAGGC ACAGCTGGAC AAGCTGGGAA CCAGGAAGGA GGAGGTGTCG
    GAAAAGTGGG ACCGCCATTG GGAGTGGCTG CAGCAGATGC TGGAGGTGCA CCAGTTTGCC
    CAGGAGGCGG TGGTGGCTGA TGCCTGGCTG ACAGCCCAGG AGCCGCTCCT GCAGAGCCGG
    GAGCTGGGCA GCAGCGTGGA TGAGGTGGAG CAGCTTATCC GGCGACATGA GGCCTTCCGC
    AAAGCGGCTG CAGCCTGGGA AGAGAGGTTC AGCTCTCTGC GGCGCCTGAC CACGATCGAG
    AAAATCAAAG CGGAACAGAG CAAGCAGCCG CCTACCCCAC TGCTGGGGCG CAAGTTCTTT
    GGGGACCCCA CGGAACTGGC GGCCAAGGCG GCGCCCCTGC TGCGGCCAGG GGGCTATGAA
    AGGGGCTTGG AGCCCCTGGC CCGCCGAGCC TCGGACACGC TCTCGGCCGA GGTGCGGACT
    CGGGTGGGGT ATGTGCGCCA GGAGCTCAAG CCCGAGCGCC TCCAGCCGCG CATTGACCGG
    CTGCCGGAGA TCCCGGGGAG GGTGGAGCCC GCGGCCCTGC CGGCCGCACC AGAGGACGCG
    GCGGAGACCC CCGCGACCCC CGCGGCGGCG GAGCAGGTGC GGCCACGACC GGAGCGCCAG
    GAGTCAGCTG ATCGCGCGGA GGAGCTGCCC AGGAGGCGGC GGCCTGAGCG GCAAGAGTCA
    GTCGATCAAT CCGAGGAGGC TGCGCGGAGG CGGCGGCCGG AGCGGCAGGA GTCAGCGGAG
    CACGAGGCGG CACACAGCCT TACCCTGGGC CGCTATGAGC AGATGGAGCG GCGGCGCGAG
    CGGCGTGAGC GGCGCTTGGA GCGGCAGGAG TCCAGCGAAC AGGAGATGCC CATCAGAGGA
    GACCTGGTCA AGGGGAAGGC CACCCTGGCT GACATTGTGG AACAGCTGCA GGAGAAAGAG
    GCAGGCCCAG GGCTGCCTGC TGGGCCGTCG CTGCCTCAGC CACGCGAGCT TCCCCCAGGT
    CGCCTGCCCA ACGGGCTTGA GCTGCCCGAG CGGACACCTC GGCCGGACCG GCCCCGGGCG
    CGGGACCGGC CCAAGCCGCG ACGGCGGCCG CGGCCCAGAG AGGGTGGTGA GGGCGGGGGA
    AGCCGGCGCT CGCGCTCCGC CCCGGCCCAG GGCGGCTCCG CCCCCGCGCC TCCGCCACCG
    CCCACTCACA CAGTGCAGCA CGAGGGCTTC CTACTGCGCA AGCGCGAGCT CGACGCTAAC
    CGCAAGTCGT CCAACCGGTC GTGGGTGAGC CTGTACTGTG TGCTTAGTAA GGGGGAACTG
    GGCTTCTACA AGGACTCCAA GGGCCCGGCA TCCGGGAGCA CACACGGTGG GGAACCGCTG
    CTCAGCCTGC ACAAGGCCAC CAGCGAGGTG GCTAGTGACT ACAAGAAAAA GAAGCATGTC
    TTCAAGCTCC AGACCCAGGA TGGCAGTGAG TTTTTGCTCC AGGCAAAAGA TGAGGAGGAG
    ATGAACGGCT GGCTGGAGGC TGTAGCTTCC TCGGTGGCGG AACACGCAGA GATCGCCCGC
    TGGGGCCAGA CACTACCCAC TACTTCATCC ACAGATGAGG GCAACCCTAA GAGGGAAGGC
    GGAGATCGCA GGGCCAGCGG GCGCAGGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016882539.1
    CDS80..7432
    Translation

    Target ORF information:

    RefSeq Version XM_017027050.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027050.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    ATGCGGATCC ACTCACTGGA GAACGTGGAC AAGGCGCTGC AGTTTCTGAA GGAGCAGCGC 
    GTGCACCTGG AGAACGTGGG TTCGCATGAC ATCGTGGATG GGAATCACCG GCTGACGCTG
    GGGCTGGTCT GGACCATCAT CCTGCGCTTC CAGATTCAAG TCATCAAAAT TGAGACTGAG
    GACAACAGAG AGACACGCTC AGCCAAGGAT GCTCTGCTCT TGTGGTGTCA GATGAAGACA
    GCTGGTTACC CTGAGGTAAA CATCCAGAAT TTCACCACCA GCTGGCGGGA TGGCTTGGCC
    TTCAATGCCC TCATTCACCG GCACAGGCCT GATCTCGTGG ACTTCAGCAA ACTCACCAAG
    TCCAATGCCA ACTACAACCT GCAGAGAGCC TTCCGCACAG CTGAGCAGCA CCTGGGGCTG
    GCGCGGCTGC TGGATCCTGA AGATGTGAAC ATGGAGGCTC CAGATGAGAA GTCCATCATC
    ACCTACGTGG TCTCTTTCTA CCACTATTTC TCCAAGATGA AGGCTCTGGC TGTGGAGGGG
    AAGCGTATCG GGAAGGTCTT GGACCAGGTA TTGGAGGTGG GGAAGATCAT AGAACGCTAC
    GAGGAGCTGG CGGCTGAGCT GCTGGCCTGG ATCCACCGCA CCGTGGGCCT CATCAGCAAT
    CAGAAATTTG CCAACTCCTT AAGTGGGGTG CAGCAGCAAC TCCAGGCTTT CACGGCCTAT
    TGCACGCTGG AGAAGCCTGT CAAGTTCCAG GAGAAGGGGA ACCTAGAGGT GCTGCTCTTC
    AGCATCCAGA GCAAACTGCG TGCCTGCAAC CGTCGCCTCT TTGTGCCTCG GGAGGGCTGT
    GGCATCTGGG ATATTGACAA GGCATGGGGT GAGCTGGAGA AGGCTGAGCA TGAGCGGGAG
    GCTGCCCTAC GGGCTGAGCT GATTCGGCAG GAGAAGCTGG AACTACTGGC ACAGAGGTTT
    GACCACAAGG TGGCTATGAG GGAGAGCTGG CTGAATGAGA ACCAGCGTCT GGTCTCCCAG
    GACAACTTTG GGTATGAGCT GCCCGCAGTG GAGGCAGCCA TGAAGAAACA CGAAGCGATC
    GAGGCAGACA TTGCGGCCTA CGAGGAGCGG GTGCAGGGTG TGGCGGAGCT GGCCCAGGCA
    TTGGCAGCCG AAGGCTACTA CGATATCCGG CGGGTGGCAG CCCAGCGTGA CAGCGTCCTG
    CGCCAGTGGG CCCTGCTAAC TGGGCTTGTG GGTGCCCGGC GGACACGACT TGAGCAGAAC
    CTTGCCCTGC AGAAGGTCTT CCAGGAGATG GTGTACATGG TGGACTGGAT GGAGGAGATG
    CAGGCTCAGC TGCTGTCCCG GGAGTGTGGG CAGCACCTGG TGGAGGCAGA CGACCTGTTG
    CAGAAGCATG GACTGCTGGA GGGAGACATT GCCGCCCAGA GCGAGCGGGT GGAGGCTCTC
    AATGCCGCTG CCCTGCGCTT CTCCCAGCTG CAGGGCTACC AGCCCTGCGA CCCGCAGGTC
    ATCTGCAACC GCGTGAACCA CGTGCACGGC TGCCTGGCGG AGCTGCAGGA GCAGGCAGCG
    CGGCGACGCG CGGAGCTGGA GGCTTCGCGG AGCCTGTGGG CGCTGCTGCA GGAGCTGGAG
    GAGGCCGAGA GCTGGGCGCG CGACAAGGAG CGTCTCCTGG AGGCTGCGGG CGGCGGCGGT
    GCGGCGGGCG CAGCGGGCGC AGCGGGAACA GCGGGCGGCG CGCATGACCT GTCCAGCACA
    GCGCGCCTCC TGGCCCAGCA CAAGATCCTG CAGGGCGAGC TGGGCGGGCG GCGAGCGTTG
    CTGCAGCAGG CCCTGCGGTG TGGCGAGGAG CTGGTTGCGG CCGGCGGTGC CGTCGGCCCG
    GGAGCAGACA CCGTGCACCT GGTAGGCCTG GCGGAGCGCG CGGCGAGCGC CCGGCGCCGC
    TGGCAGAGGC TGGAAGAGGC GGCGGCGCGG CGAGAGCGGC GGCTGCAGGA GGCGCGGGCG
    CTGCACCAGT TCGGCGCTGA CCTCGACGGG CTGCTGGACT GGCTTCGCGA CGCTTACCGC
    CTGGCAGCCG CCGGTGACTT CGGCCACGAC GAAGCTTCCA GCCGCCGCCT GGCGCGCCAG
    CACCGCGCGC TCACCGGGGA GGTGGAGGCA CATCGCGGGC CCGTGAGCGG CCTGCGGCGC
    CAGCTGGCGA CACTCGGGGG TGCCAGTGGC GCAGGGCCAC TGGTGGTGGC GCTGCAGGTG
    CGCGTGGTGG AAGCAGAGCA GTTGTTCGCT GAGGTGACCG AAGTGGCGGC GCTGAGGCGC
    CAGTGGCTGC GGGACGCGCT CGCTGTCTAC CGCATGTTTG GCGAGGTGCA CGCGTGTGAG
    CTGTGGATCG GCGAGAAGGA GCAATGGCTG CTCTCCATGC GTGTGCCGGA TTCACTCGAC
    GACGTCGAGG TGGTGCAGCA CCGATTCGAG AGCCTGGACC AAGAGATGAA CAGCCTGATG
    GGCCGCGTTC TGGACGTGAA CCACACAGTC CAGGAGCTGG TGGAAGGAGG CCACCCCAGT
    TCAGATGAGG TGCGTTCCTG CCAGGACCAC CTCAACAGCA GGTGGAACCG CATCGTGGAG
    CTAGTGGAAC AGCGCAAAGA GGAAATGAGC GCGGTGCTGC TGGTGGAGAA CCACGTGCTG
    GAGGTGGCCG AGGTGCGCGC CCAGGTGCGT GAGAAGCGGA GAGCTGTGGA GAGCGCGCCC
    CGGGCCGGCG GCGCCCTGCA GTGGCGTCTT AGCGGCCTAG AGGCCGCTCT GCAGGCGCTG
    GAGCCGCGCC AGGCGGCCCT TCTGGAGGAG GCAGCCCTGC TGGCTGAGCG CTTCCCGGCG
    CAGGCGGCGC GGCTGCACCA GGGCGCGGAG GAGCTGGGCG CCGAGTGGGG CGCGCTAGCT
    AGCGCGGCTC AGGCCTGCGG CGAGGCGGTG GCGGCAGCAG GGCGCCTGCA GCGCTTCCTA
    CATGACCTCG ACGCTTTCCT GGACTGGCTC GTGCGCGCCC AGGAGGCGGC GGGCGGCAGC
    GAGGGGCCCC TGCCCAACAG CCTAGAAGAG GCGGACGCGC TGCTGGCGCG CCACGCTGCG
    CTCAAGGAGG AGGTGGACCA GCGCGAGGAA GACTATGCTC GCATCGTGGC GGCCAGCGAG
    GCGCTGCTGG CCGCCGACGG CGCAGAGCTG GGCCCGGGCC TGGCACTAGA CGAGTGGCTG
    CCACACCTCG AACTTGGCTG GCATAAACTG CTCGGCTTGT GGGAGGCGCG CAGGGAGGCG
    CTGGTCCAGG CGCACATCTA CCAGCTCTTC CTGCGGGATC TACGCCAGGC GCTCGTGGTG
    CTGCGTAACC AGGAGATGGC GCTGTCTGGT GCGGAGCTCC CGGGCACAGT GGAATCGGTG
    GAGGAGGCCT TGAAACAGCA CCGTGACTTT CTCACCACCA TGGAGCTGAG CCAGCAAAAG
    ATGCAGGTGG CCGTGCAGGC TGCAGAGGGC CTGCTGAGGC AGGGCAACAT CTACGGGGAG
    CAGGCTCAGG AGGCTGTGAC CCGGCTGCTG GAGAAGAACC AAGAAAACCA GTTACGGGCC
    CAGCAATGGA TGCAAAAGCT ACATGACCAA CTTGAGCTGC AGCACTTCCT CCGAGACTGC
    CACGAGCTGG ATGGCTGGAT CCATGAGAAG ATGCTGATGG CGCGGGATGG CACGCGGGAG
    GACAACCACA AGCTGCATAA GAGATGGCTC CGGCACCAGG CATTCATGGC CGAGCTGGCT
    CAGAATAAGG AGTGGCTGGA GAAGATCGAG CGGGAGGGCC AGCAACTGAT GCAGGAGAAG
    CCCGAACTGG CGGCCTCCGT GCGGAAGAAG CTGGGCGAGA TCCGCCAGTG CTGGGCGGAG
    CTGGAGAGCA CCACCCAGGC CAAGGCACGG CAGCTCTTTG AGGCCAGCAA AGCAGACCAG
    CTGGTGCAGA GCTTTGCTGA GCTGGACAAG AAGCTCCTTC ACATGGAGAG CCAGCTGCAA
    GACGTGGACC CTGGAGGAGA CCTGGCCACT GTCAACAGTC AGCTCAAGAA GCTGCAGTCC
    ATGGAGTCGC AGGTGGAGGA GTGGTACCGC GAGGTGGGAG AGCTGCAGGC GCAGACGGCG
    GCGCTGCCGC TGGAGCCGGC GAGCAAGGAG CTGGTGGGTG AGCGGCAGAA CGCGGTGGGC
    GAGCGCCTGG TGCGCCTGCT CGAGCCGTTG CAGGAGCGCC GCCGCTTGCT GCTGGCTTCC
    AAGGAGTTGC ACCAGGTGGC GCACGACCTG GACGACGAGC TGGCATGGGT TCAGGAGCGG
    CTGCCACTGG CCATGCAGAC AGAGCGAGGC AACGGTTTGC AGGCGGTCCA GCAGCACATC
    AAAAAGAACC AGGGCCTGCG GCGGGAGATC CAGGCGCATG GGCCGCGCCT GGAGGAGGTG
    CTGGAGCGCG CGGGCGCGCT GGCGTCGCTG CGCAGCCCGG AGGCAGAGGC AGTGCGCCGG
    GGCCTGGAGC AGCTGCAGAG CGCCTGGGCC GGACTGCGGG AGGCTGCCGA GCGACGGCAG
    CAGGTGCTGG ACGCCGCCTT CCAGGTGGAG CAGTACTACT TCGACGTGGC TGAGGTGGAG
    GCGTGGCTGG GCGAGCAGGA GCTGCTCATG ATGAGTGAGG ACAAGGGCAA GGACGAACAG
    AGCACCCTGC AGCTGCTCAA GAAACACCTG CAGCTGGAGC AAGGCGTGGA GAACTACGAG
    GAAAGCATCG CGCAGCTGTC GCGCCAGTGC CGGGCGCTGC TGGAGATGGG GCACCCGGAC
    AGCGAGCAGA TCAGCCGGCG GCAGTCTCAG GTGGACCGCC TGTACGTGGC GCTCAAGGAG
    CTGGGTGAGG AGCGCCGGGT GGCTCTGGAA CAGCAGTACT GGCTGTACCA GCTCAGCCGC
    CAGGTGAGCG AGCTTGAGCA CTGGATTGCC GAGAAGGAGG TGGTGGCTGG CTCACCCGAG
    CTCGGCCAGG ACTTTGAGCA TGTCTCGGTG CTGCAGGAGA AATTCTCAGA GTTTGCCAGC
    GAGACAGGTA TGGCAGGGCG GGAACGGCTG GCAGCTGTGA ACCAGATGGT GGATGAGCTG
    ATCGAGTGTG GCCATACAGC AGCGGCCACC ATGGCCGAGT GGAAGGACGG ACTGAACGAG
    GCCTGGGCTG AGCTGCTGGA GCTCATGGGC ACACGGGCCC AGCTGCTGGC CGCCTCTCGG
    GAGCTTCATA AGTTCTTCAG TGACGCCCGA GAGCTTCAGG GACAGATTGA GGAGAAGCGG
    AGGCGGCTGC CCCGCCTGAC CACCCCGCCT GAGCCGAGAC CCAGTGCCAG TTCCATGCAG
    CGGACCCTGA GAGCCTTTGA GCATGACCTG CAGCTCCTCG TGTCCCAGGT ACGGCAGCTG
    CAGGAGGGGG CGGCCCAGCT GCGGACGGTG TATGCGGGTG AACATGCCGA GGCCATCGCT
    AGCCGGGAGC AGGAGGTGCT GCAGGGTTGG AAAGAGCTGC TGTCAGCCTG TGAGGATGCC
    CGCCTGCATG TCAGCTCCAC AGCCGACGCC CTGCGCTTCC ACAGCCAAGT CCGCGACCTG
    CTCTCCTGGA TGGATGGCAT CGCCAGCCAG ATTGGGGCAG CCGACAAGCC CAGGGACGTG
    TCATCAGTGG AGGTGCTCAT GAACTACCAC CAGGGCCTGA AGACTGAGCT GGAGGCGCGG
    GTGCCTGAGC TGACCACCTG CCAGGAGCTG GGGCGATCTC TGCTGCTCAA CAAAAGTGCC
    ATGGCTGATG AGATCCAGGC ACAGCTGGAC AAGCTGGGAA CCAGGAAGGA GGAGGTGTCG
    GAAAAGTGGG ACCGCCATTG GGAGTGGCTG CAGCAGATGC TGGAGGTGCA CCAGTTTGCC
    CAGGAGGCGG TGGTGGCTGA TGCCTGGCTG ACAGCCCAGG AGCCGCTCCT GCAGAGCCGG
    GAGCTGGGCA GCAGCGTGGA TGAGGTGGAG CAGCTTATCC GGCGACATGA GGCCTTCCGC
    AAAGCGGCTG CAGCCTGGGA AGAGAGGTTC AGCTCTCTGC GGCGCCTGAC CACGATCGAG
    AAAATCAAAG CGGAACAGAG CAAGCAGCCG CCTACCCCAC TGCTGGGGCG CAAGTTCTTT
    GGGGACCCCA CGGAACTGGC GGCCAAGGCG GCGCCCCTGC TGCGGCCAGG GGGCTATGAA
    AGGGGCTTGG AGCCCCTGGC CCGCCGAGCC TCGGACACGC TCTCGGCCGA GGTGCGGACT
    CGGGTGGGGT ATGTGCGCCA GGAGCTCAAG CCCGAGCGCC TCCAGCCGCG CATTGACCGG
    CTGCCGGAGA TCCCGGGGAG GGTGGAGCCC GCGGCCCTGC CGGCCGCACC AGAGGACGCG
    GCGGAGACCC CCGCGACCCC CGCGGCGGCG GAGCAGGTGC GGCCACGACC GGAGCGCCAG
    GAGTCAGCTG ATCGCGCGGA GGAGCTGCCC AGGAGGCGGC GGCCTGAGCG GCAAGAGTCA
    GTCGATCAAT CCGAGGAGGC TGCGCGGAGG CGGCGGCCGG AGCGGCAGGA GTCAGCGGAG
    CACGAGGCGG CACACAGCCT TACCCTGGGC CGCTATGAGC AGATGGAGCG GCGGCGCGAG
    CGGCGTGAGC GGCGCTTGGA GCGGCAGGAG TCCAGCGAAC AGGAGATGCC CATCAGAGGA
    GACCTGGTCA AGGGGAAGGC CACCCTGGCT GACATTGTGG AACAGCTGCA GGAGAAAGAG
    GCAGGCCCAG GGCTGCCTGC TGGGCCGTCG CTGCCTCAGC CACGCGAGCT TCCCCCAGGT
    CGCCTGCCCA ACGGGCTTGA GCTGCCCGAG CGGACACCTC GGCCGGACCG GCCCCGGGCG
    CGGGACCGGC CCAAGCCGCG ACGGCGGCCG CGGCCCAGAG AGGGTGGTGA GGGCGGGGGA
    AGCCGGCGCT CGCGCTCCGC CCCGGCCCAG GGCGGCTCCG CCCCCGCGCC TCCGCCACCG
    CCCACTCACA CAGTGCAGCA CGAGGGCTTC CTACTGCGCA AGCGCGAGCT CGACGCTAAC
    CGCAAGTCGT CCAACCGGTC GTGGGTGAGC CTGTACTGTG TGCTTAGTAA GGGGGAACTG
    GGCTTCTACA AGGACTCCAA GGGCCCGGCA TCCGGGAGCA CACACGGTGG GGAACCGCTG
    CTCAGCCTGC ACAAGGCCAC CAGCGAGGTG GCTAGTGACT ACAAGAAAAA GAAGCATGTC
    TTCAAGCTCC AGACCCAGGA TGGCAGTGAG TTTTTGCTCC AGGCAAAAGA TGAGGAGGAG
    ATGAACGGCT GGCTGGAGGC TGTAGCTTCC TCGGTGGCGG AACACGCAGA GATCGCCCGC
    TGGGGCCAGA CACTACCCAC TACTTCATCC ACAGATGAGG GCAACCCTAA GAGGGAAGGC
    GGAGATCGCA GGGCCAGCGG GCGCAGGAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88602
    Clone ID Related Accession (Same CDS sequence) XM_017027051.1
    Accession Version XM_017027051.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 6390bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
    CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
    ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
    GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
    CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
    GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
    GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
    GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
    ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
    TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
    AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
    CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
    CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
    GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
    TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
    TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
    CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
    TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
    GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
    CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
    AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
    CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
    AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
    CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
    TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
    TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
    ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
    TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
    CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
    GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
    TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
    CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
    GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
    CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
    GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
    CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
    TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
    CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
    GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
    GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
    TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
    GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
    GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
    CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
    CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
    GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
    CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
    AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
    TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
    GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
    GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
    CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
    CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
    CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
    GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
    CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
    AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
    CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
    GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
    TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
    TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
    GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
    CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
    GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
    ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
    CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
    ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
    AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
    GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
    GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
    GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
    GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
    GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
    GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
    GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
    CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
    GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
    ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
    CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
    CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
    AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
    TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
    GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
    AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
    TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
    CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
    GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
    CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
    CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
    CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
    GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
    GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
    AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
    ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
    GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
    CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
    CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
    ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
    ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
    ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
    TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
    GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
    TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
    GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
    GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
    GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGGCGG GAGGATCACT TGAACCCAGG
    AGTTCAAGAC CAGCCTTGGC AACATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016882540.1
    CDS169..6558
    Translation

    Target ORF information:

    RefSeq Version XM_017027051.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027051.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
    CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
    ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
    GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
    CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
    GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
    GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
    GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
    ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
    TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
    AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
    CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
    CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
    GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
    TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
    TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
    CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
    TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
    GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
    CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
    AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
    CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
    AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
    CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
    TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
    TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
    ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
    TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
    CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
    GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
    TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
    CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
    GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
    CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
    GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
    CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
    TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
    CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
    GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
    GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
    TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
    GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
    GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
    CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
    CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
    GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
    CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
    AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
    TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
    GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
    GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
    CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
    CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
    CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
    GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
    CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
    AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
    CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
    GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
    TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
    TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
    GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
    CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
    GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
    ACCCGGCTGC TGGAGAAGAA CCAAGAAAAC CAGTTACGGG CCCAGCAATG GATGCAAAAG
    CTACATGACC AACTTGAGCT GCAGCACTTC CTCCGAGACT GCCACGAGCT GGATGGCTGG
    ATCCATGAGA AGATGCTGAT GGCGCGGGAT GGCACGCGGG AGGACAACCA CAAGCTGCAT
    AAGAGATGGC TCCGGCACCA GGCATTCATG GCCGAGCTGG CTCAGAATAA GGAGTGGCTG
    GAGAAGATCG AGCGGGAGGG CCAGCAACTG ATGCAGGAGA AGCCCGAACT GGCGGCCTCC
    GTGCGGAAGA AGCTGGGCGA GATCCGCCAG TGCTGGGCGG AGCTGGAGAG CACCACCCAG
    GCCAAGGCAC GGCAGCTCTT TGAGGCCAGC AAAGCAGACC AGCTGGTGCA GAGCTTTGCT
    GAGCTGGACA AGAAGCTCCT TCACATGGAG AGCCAGCTGC AAGACGTGGA CCCTGGAGGA
    GACCTGGCCA CTGTCAACAG TCAGCTCAAG AAGCTGCAGT CCATGGAGTC GCAGGTGGAG
    GAGTGGTACC GCGAGGTGGG AGAGCTGCAG GCGCAGACGG CGGCGCTGCC GCTGGAGCCG
    GCGAGCAAGG AGCTGGTGGG TGAGCGGCAG AACGCGGTGG GCGAGCGCCT GGTGCGCCTG
    CTCGAGCCGT TGCAGGAGCG CCGCCGCTTG CTGCTGGCTT CCAAGGAGTT GCACCAGGTG
    GCGCACGACC TGGACGACGA GCTGGCATGG GTTCAGGAGC GGCTGCCACT GGCCATGCAG
    ACAGAGCGAG GCAACGGTTT GCAGGCGGTC CAGCAGCACA TCAAAAAGAA CCAGGGCCTG
    CGGCGGGAGA TCCAGGCGCA TGGGCCGCGC CTGGAGGAGG TGCTGGAGCG CGCGGGCGCG
    CTGGCGTCGC TGCGCAGCCC GGAGGCAGAG GCAGTGCGCC GGGGCCTGGA GCAGCTGCAG
    AGCGCCTGGG CCGGACTGCG GGAGGCTGCC GAGCGACGGC AGCAGGTGCT GGACGCCGCC
    TTCCAGGTGG AGCAGTACTA CTTCGACGTG GCTGAGGTGG AGGCGTGGCT GGGCGAGCAG
    GAGCTGCTCA TGATGAGTGA GGACAAGGGC AAGGACGAAC AGAGCACCCT GCAGCTGCTC
    AAGAAACACC TGCAGCTGGA GCAAGGCGTG GAGAACTACG AGGAAAGCAT CGCGCAGCTG
    TCGCGCCAGT GCCGGGCGCT GCTGGAGATG GGGCACCCGG ACAGCGAGCA GATCAGCCGG
    CGGCAGTCTC AGGTGGACCG CCTGTACGTG GCGCTCAAGG AGCTGGGTGA GGAGCGCCGG
    GTGGCTCTGG AACAGCAGTA CTGGCTGTAC CAGCTCAGCC GCCAGGTGAG CGAGCTTGAG
    CACTGGATTG CCGAGAAGGA GGTGGTGGCT GGCTCACCCG AGCTCGGCCA GGACTTTGAG
    CATGTCTCGG TGCTGCAGGA GAAATTCTCA GAGTTTGCCA GCGAGACAGG TATGGCAGGG
    CGGGAACGGC TGGCAGCTGT GAACCAGATG GTGGATGAGC TGATCGAGTG TGGCCATACA
    GCAGCGGCCA CCATGGCCGA GTGGAAGGAC GGACTGAACG AGGCCTGGGC TGAGCTGCTG
    GAGCTCATGG GCACACGGGC CCAGCTGCTG GCCGCCTCTC GGGAGCTTCA TAAGTTCTTC
    AGTGACGCCC GAGAGCTTCA GGGACAGATT GAGGAGAAGC GGAGGCGGCT GCCCCGCCTG
    ACCACCCCGC CTGAGCCGAG ACCCAGTGCC AGTTCCATGC AGCGGACCCT GAGAGCCTTT
    GAGCATGACC TGCAGCTCCT CGTGTCCCAG GTACGGCAGC TGCAGGAGGG GGCGGCCCAG
    CTGCGGACGG TGTATGCGGG TGAACATGCC GAGGCCATCG CTAGCCGGGA GCAGGAGGTG
    CTGCAGGGTT GGAAAGAGCT GCTGTCAGCC TGTGAGGATG CCCGCCTGCA TGTCAGCTCC
    ACAGCCGACG CCCTGCGCTT CCACAGCCAA GTCCGCGACC TGCTCTCCTG GATGGATGGC
    ATCGCCAGCC AGATTGGGGC AGCCGACAAG CCCAGGGACG TGTCATCAGT GGAGGTGCTC
    ATGAACTACC ACCAGGGCCT GAAGACTGAG CTGGAGGCGC GGGTGCCTGA GCTGACCACC
    TGCCAGGAGC TGGGGCGATC TCTGCTGCTC AACAAAAGTG CCATGGCTGA TGAGATCCAG
    GCACAGCTGG ACAAGCTGGG AACCAGGAAG GAGGAGGTGT CGGAAAAGTG GGACCGCCAT
    TGGGAGTGGC TGCAGCAGAT GCTGGAGGTG CACCAGTTTG CCCAGGAGGC GGTGGTGGCT
    GATGCCTGGC TGACAGCCCA GGAGCCGCTC CTGCAGAGCC GGGAGCTGGG CAGCAGCGTG
    GATGAGGTGG AGCAGCTTAT CCGGCGACAT GAGGCCTTCC GCAAAGCGGC TGCAGCCTGG
    GAAGAGAGGT TCAGCTCTCT GCGGCGCCTG ACCACGGCGG GAGGATCACT TGAACCCAGG
    AGTTCAAGAC CAGCCTTGGC AACATACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88603
    Clone ID Related Accession (Same CDS sequence) XM_017027052.1
    Accession Version XM_017027052.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3930bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product spectrin beta chain, non-erythrocytic 4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
    CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
    ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
    GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
    CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
    GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
    GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
    GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
    ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
    TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
    AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
    CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
    CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
    GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
    TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
    TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
    CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
    TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
    GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
    CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
    AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
    CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
    AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
    CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
    TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
    TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
    ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
    TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
    CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
    GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
    TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
    CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
    GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
    CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
    GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
    CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
    TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
    CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
    GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
    GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
    TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
    GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
    GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
    CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
    CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
    GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
    CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
    AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
    TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
    GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
    GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
    CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
    CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
    CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
    GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
    CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
    AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
    CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
    GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
    TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
    TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
    GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
    CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
    GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
    ACCCGGCTGC TGGAGAAATG CCTCATTATC CATCCTGCTC TTCTGCACCC TCCTTGGGAA
    CCCCCAGACC TCCCCAGATC CAGCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016882541.1
    CDS169..4098
    Translation

    Target ORF information:

    RefSeq Version XM_017027052.1
    Organism Homo sapiens(human)
    Definition Homo sapiens spectrin beta, non-erythrocytic 4 (SPTBN4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027052.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGCGCAGG TACCAGGGGA AGTGGACAAC ATGGAGGGCC TGCCTGCTCC TAACAACAAC 
    CCTGCTGCCC GCTGGGAGAG TCCGGATCGG GGCTGGGAGC GGGAGCAGCC GGCTGCGTCC
    ACCGCAGCGG CCTCGCTCTT TGAGTGCTCC CGGATCAAGG CCTTGGCAGA TGAGCGGGAA
    GCCGTGCAGA AGAAAACCTT CACCAAGTGG GTGAACTCGC ACCTCGCCCG CGTGGGCTGC
    CACATCGGGG ACCTCTATGT GGACCTCCGG GACGGCTTCG TGCTCACGCG GCTCCTGGAA
    GTGCTGTCTG GGGAGCAGCT GCCCAGGCCC ACGCGCGGCC GCATGCGGAT CCACTCACTG
    GAGAACGTGG ACAAGGCGCT GCAGTTTCTG AAGGAGCAGC GCGTGCACCT GGAGAACGTG
    GGTTCGCATG ACATCGTGGA TGGGAATCAC CGGCTGACGC TGGGGCTGGT CTGGACCATC
    ATCCTGCGCT TCCAGATTCA AGTCATCAAA ATTGAGACTG AGGACAACAG AGAGACACGC
    TCAGCCAAGG ATGCTCTGCT CTTGTGGTGT CAGATGAAGA CAGCTGGTTA CCCTGAGGTA
    AACATCCAGA ATTTCACCAC CAGCTGGCGG GATGGCTTGG CCTTCAATGC CCTCATTCAC
    CGGCACAGGC CTGATCTCGT GGACTTCAGC AAACTCACCA AGTCCAATGC CAACTACAAC
    CTGCAGAGAG CCTTCCGCAC AGCTGAGCAG CACCTGGGGC TGGCGCGGCT GCTGGATCCT
    GAAGATGTGA ACATGGAGGC TCCAGATGAG AAGTCCATCA TCACCTACGT GGTCTCTTTC
    TACCACTATT TCTCCAAGAT GAAGGCTCTG GCTGTGGAGG GGAAGCGTAT CGGGAAGGTC
    TTGGACCAGG TATTGGAGGT GGGGAAGATC ATAGAACGCT ACGAGGAGCT GGCGGCTGAG
    CTGCTGGCCT GGATCCACCG CACCGTGGGC CTCATCAGCA ATCAGAAATT TGCCAACTCC
    TTAAGTGGGG TGCAGCAGCA ACTCCAGGCT TTCACGGCCT ATTGCACGCT GGAGAAGCCT
    GTCAAGTTCC AGGAGAAGGG GAACCTAGAG GTGCTGCTCT TCAGCATCCA GAGCAAACTG
    CGTGCCTGCA ACCGTCGCCT CTTTGTGCCT CGGGAGGGCT GTGGCATCTG GGATATTGAC
    AAGGCATGGG GTGAGCTGGA GAAGGCTGAG CATGAGCGGG AGGCTGCCCT ACGGGCTGAG
    CTGATTCGGC AGGAGAAGCT GGAACTACTG GCACAGAGGT TTGACCACAA GGTGGCTATG
    AGGGAGAGCT GGCTGAATGA GAACCAGCGT CTGGTCTCCC AGGACAACTT TGGGTATGAG
    CTGCCCGCAG TGGAGGCAGC CATGAAGAAA CACGAAGCGA TCGAGGCAGA CATTGCGGCC
    TACGAGGAGC GGGTGCAGGG TGTGGCGGAG CTGGCCCAGG CATTGGCAGC CGAAGGCTAC
    TACGATATCC GGCGGGTGGC AGCCCAGCGT GACAGCGTCC TGCGCCAGTG GGCCCTGCTA
    ACTGGGCTTG TGGGTGCCCG GCGGACACGA CTTGAGCAGA ACCTTGCCCT GCAGAAGGTC
    TTCCAGGAGA TGGTGTACAT GGTGGACTGG ATGGAGGAGA TGCAGGCTCA GCTGCTGTCC
    CGGGAGTGTG GGCAGCACCT GGTGGAGGCA GACGACCTGT TGCAGAAGCA TGGACTGCTG
    GAGGGAGACA TTGCCGCCCA GAGCGAGCGG GTGGAGGCTC TCAATGCCGC TGCCCTGCGC
    TTCTCCCAGC TGCAGGGCTA CCAGCCCTGC GACCCGCAGG TCATCTGCAA CCGCGTGAAC
    CACGTGCACG GCTGCCTGGC GGAGCTGCAG GAGCAGGCAG CGCGGCGACG CGCGGAGCTG
    GAGGCTTCGC GGAGCCTGTG GGCGCTGCTG CAGGAGCTGG AGGAGGCCGA GAGCTGGGCG
    CGCGACAAGG AGCGTCTCCT GGAGGCTGCG GGCGGCGGCG GTGCGGCGGG CGCAGCGGGC
    GCAGCGGGAA CAGCGGGCGG CGCGCATGAC CTGTCCAGCA CAGCGCGCCT CCTGGCCCAG
    CACAAGATCC TGCAGGGCGA GCTGGGCGGG CGGCGAGCGT TGCTGCAGCA GGCCCTGCGG
    TGTGGCGAGG AGCTGGTTGC GGCCGGCGGT GCCGTCGGCC CGGGAGCAGA CACCGTGCAC
    CTGGTAGGCC TGGCGGAGCG CGCGGCGAGC GCCCGGCGCC GCTGGCAGAG GCTGGAAGAG
    GCGGCGGCGC GGCGAGAGCG GCGGCTGCAG GAGGCGCGGG CGCTGCACCA GTTCGGCGCT
    GACCTCGACG GGCTGCTGGA CTGGCTTCGC GACGCTTACC GCCTGGCAGC CGCCGGTGAC
    TTCGGCCACG ACGAAGCTTC CAGCCGCCGC CTGGCGCGCC AGCACCGCGC GCTCACCGGG
    GAGGTGGAGG CACATCGCGG GCCCGTGAGC GGCCTGCGGC GCCAGCTGGC GACACTCGGG
    GGTGCCAGTG GCGCAGGGCC ACTGGTGGTG GCGCTGCAGG TGCGCGTGGT GGAAGCAGAG
    CAGTTGTTCG CTGAGGTGAC CGAAGTGGCG GCGCTGAGGC GCCAGTGGCT GCGGGACGCG
    CTCGCTGTCT ACCGCATGTT TGGCGAGGTG CACGCGTGTG AGCTGTGGAT CGGCGAGAAG
    GAGCAATGGC TGCTCTCCAT GCGTGTGCCG GATTCACTCG ACGACGTCGA GGTGGTGCAG
    CACCGATTCG AGAGCCTGGA CCAAGAGATG AACAGCCTGA TGGGCCGCGT TCTGGACGTG
    AACCACACAG TCCAGGAGCT GGTGGAAGGA GGCCACCCCA GTTCAGATGA GGTGCGTTCC
    TGCCAGGACC ACCTCAACAG CAGGTGGAAC CGCATCGTGG AGCTAGTGGA ACAGCGCAAA
    GAGGAAATGA GCGCGGTGCT GCTGGTGGAG AACCACGTGC TGGAGGTGGC CGAGGTGCGC
    GCCCAGGTGC GTGAGAAGCG GAGAGCTGTG GAGAGCGCGC CCCGGGCCGG CGGCGCCCTG
    CAGTGGCGTC TTAGCGGCCT AGAGGCCGCT CTGCAGGCGC TGGAGCCGCG CCAGGCGGCC
    CTTCTGGAGG AGGCAGCCCT GCTGGCTGAG CGCTTCCCGG CGCAGGCGGC GCGGCTGCAC
    CAGGGCGCGG AGGAGCTGGG CGCCGAGTGG GGCGCGCTAG CTAGCGCGGC TCAGGCCTGC
    GGCGAGGCGG TGGCGGCAGC AGGGCGCCTG CAGCGCTTCC TACATGACCT CGACGCTTTC
    CTGGACTGGC TCGTGCGCGC CCAGGAGGCG GCGGGCGGCA GCGAGGGGCC CCTGCCCAAC
    AGCCTAGAAG AGGCGGACGC GCTGCTGGCG CGCCACGCTG CGCTCAAGGA GGAGGTGGAC
    CAGCGCGAGG AAGACTATGC TCGCATCGTG GCGGCCAGCG AGGCGCTGCT GGCCGCCGAC
    GGCGCAGAGC TGGGCCCGGG CCTGGCACTA GACGAGTGGC TGCCACACCT CGAACTTGGC
    TGGCATAAAC TGCTCGGCTT GTGGGAGGCG CGCAGGGAGG CGCTGGTCCA GGCGCACATC
    TACCAGCTCT TCCTGCGGGA TCTACGCCAG GCGCTCGTGG TGCTGCGTAA CCAGGAGATG
    GCGCTGTCTG GTGCGGAGCT CCCGGGCACA GTGGAATCGG TGGAGGAGGC CTTGAAACAG
    CACCGTGACT TTCTCACCAC CATGGAGCTG AGCCAGCAAA AGATGCAGGT GGCCGTGCAG
    GCTGCAGAGG GCCTGCTGAG GCAGGGCAAC ATCTACGGGG AGCAGGCTCA GGAGGCTGTG
    ACCCGGCTGC TGGAGAAATG CCTCATTATC CATCCTGCTC TTCTGCACCC TCCTTGGGAA
    CCCCCAGACC TCCCCAGATC CAGCAGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.