CHD8 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CHD8
Entrez Gene ID	57680
Full Name	chromodomain helicase DNA binding protein 8
Synonyms	AUTS18,HELSNF1
General protein information	Preferred Names chromodomain helicase DNA binding protein 8 Names chromodomain-helicase-DNA-binding protein 8 ATP-dependent helicase CHD8 axis duplication inhibitor duplin helicase with SNF2 domain 1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 14 Map Location: 14q11.2
Summary	This gene encodes a member of the chromodomain-helicase-DNA binding protein family, which is characterized by a SNF2-like domain and two chromatin organization modifier domains. The encoded protein also contains brahma and kismet domains, which are common to the subfamily of chromodomain-helicase-DNA binding proteins to which this protein belongs. This gene has been shown to function in several processes that include transcriptional regulation, epigenetic remodeling, promotion of cell proliferation, and regulation of RNA synthesis. Allelic variants of this gene are associated with autism spectrum disorder. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2016].
Disorder MIM:	610528
Disorder Html:	{Autism, susceptibility to, 18}, 615032 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001170629.1	NP_001164100.1	chromodomain-helicase-DNA-binding protein 8 isoform 1
NM_001170629.2	NP_001164100.1	chromodomain-helicase-DNA-binding protein 8 isoform 1
NM_020920.3	NP_065971.2	chromodomain-helicase-DNA-binding protein 8 isoform 2
NM_020920.4	NP_065971.2	chromodomain-helicase-DNA-binding protein 8 isoform 2

Pathways from BioSystems

Homologies

	CHD8	XP_001096619.2
Bos taurus (cattle)	CHD8	NP_001179063.1
Danio rerio (zebrafish)	chd8	NP_001189381.1
Canis lupus familiaris (dog)	CHD8	XP_532624.4
Rattus norvegicus (Norway rat)	Chd8	NP_075222.2
Pan troglodytes (chimpanzee)	CHD8	XP_003314292.1
Mus musculus (house mouse)	Chd8	NP_963999.2
Homo sapiens (human)	CHD8	NP_001164100.1
Xenopus tropicalis (tropical clawed frog)	chd8	NP_001131089.2

Gene Ontology
Bibliography

Related articles in PubMed

Enriched expression of genes associated with autism spectrum disorders in human inhibitory neurons.
Wang P, Zhao D, Lachman HM, Zheng D
Translational psychiatry8(1)13(2018 Jan)

Genome-wide association study identified copy number variants associated with sporadic colorectal cancer risk.
Thean LF, Low YS, Lo M, Teo YY, Koh WP, Yuan JM, Chew MH, Tang CL, Cheah PY
Journal of medical genetics(2017 Oct)

The ATP-dependent chromatin remodeling enzymes CHD6, CHD7, and CHD8 exhibit distinct nucleosome binding and remodeling activities.
Manning BJ, Yusufzai T
The Journal of biological chemistry292(28)11927-11936(2017 07)

A systematic variant annotation approach for ranking genes associated with autism spectrum disorders.
Larsen E, Menashe I, Ziats MN, Pereanu W, Packer A, Banerjee-Basu S
Molecular autism744(2016)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that CHD6 and CHD7 both bind with high affinity to short linker DNA, whereas CHD8 requires longer DNA for binding; thus, CHD8 slides nucleosomes into positions with more flanking linker DNA than CHD7; CHD6 disrupts nucleosomes in a distinct non-sliding manner.
Title: The ATP-dependent chromatin remodeling enzymes CHD6, CHD7, and CHD8 exhibit distinct nucleosome binding and remodeling activities.

Review of 16 other CHD8 mutation cases suggests that clinical features and their severity vary considerably across individuals; however, these data support a CHD8 mutation syndrome, further highlighting the importance of genomic medicine to guide clinical assessment and treatment
Title: A de novo frameshift mutation in chromodomain helicase DNA-binding domain 8 (CHD8): A case report and literature review.

present observation and published data suggest that phenotype present in patients with duplication of 14q11.2 region, encompassing the SUPT16H and CHD8 genes, resemble in some extend features described in cases carrying microdeletion of that genomic region
Title: Novel 14q11.2 microduplication including the CHD8 and SUPT16H genes associated with developmental delay.

Our clinical case supports the hypothesis that CHD8 may play a central role in neuronal cell development and Autism spectrum disorders risk
Title: CHD8 intragenic deletion associated with autism spectrum disorder.

SHANK3, CHD8, and ADNP had distinctly higher scores than all other genes in the dataset describing the genes associated with autism spectrum disorders.
Title: A systematic variant annotation approach for ranking genes associated with autism spectrum disorders.

The following CHD8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHD8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu15118	NM_001170629.2 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu17867	NM_020920.4 Latest version!	Homo sapiens chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
View Old Accession Versions >>
OHu15118	NM_001170629.1	Homo sapiens chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$659.50-$923.30 $1319.00
OHu17867	NM_020920.3	Homo sapiens chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$923.30 $1319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CHD8 ( NM_001170629.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001164100.1 Homo sapiens chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 1, mRNA.

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CloneID	OHu15118
Clone ID Related Accession (Same CDS sequence)	NM_001170629.1 , NM_001170629.2
Accession Version	NM_001170629.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 8 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161747.5, AB046784.3, DA327823.1, DB344386.1 and AW327785.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript and protein alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB046784.3 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACC CCATCATGGA TCTGTTCGAT GACCCAAATT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAACCA GGTCACACAA GACCCCATTG AGGAAGCCCT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAGGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGAACTGGT CCCTCCACCA GAGGAAACAG CTCCCACAGA ACTTTCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCATA ACCTTGCATG ATTATACCAC TCAGCCTGCC 
AGCCAGGAGC AGCCAGCCCA ACCTGTCTTA CAGACATCGA CGCCAACATC AGGACTTTTG 
CAAGTCTCCA AGAGCCAGGA GATCCTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGCTGTCT CCTCCAGTAG TGCTGGAGGG CAGCCACCTC AGTCAGCCCC TAAGATTGTT 
ATCCTTAAGG CCCCACCAAG CTCCTCAGTC ACTGGTGCCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGCACAGC TCAGCCCCTG GTGGCAGGCA CAGCCAATGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTAAC CGGCACACCC CTTCGACCAG GTGTTTCCAT TGTCTCTGGT 
AATACAGTGT TGGCCGCCAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCCAGCCGAC CAGTAAAGCA GCTGGTCCTC CAGCCAGTTA AGGGTTCAGC TCCTGCTGGA 
AACCCTGGGG CCACAGGGCC CCCACTGAAG CCTGCAGTTA CACTGACCTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACCCTGGTC CTCCAGCAGC CACAGTCTGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGCT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTGGCAGCCC TGACTCAAGC CAAGAATGCC CAAGGGCAGC CTGCCAAGGT AGTAACTATC 
CAGCTGCAGG TGCAGCAGCC ACAGCAAAAA ATCCAGATTG TACCACAACC ACCATCATCG 
CAGCCACAGC CCCAGCAGCC ACCCTCCACC CAGCCAGTGA CTCTGTCCTC TGTACAGCAG 
GCTCAGATAA TGGGACCAGG ACAAAGCCCA GGACAAAGAC TTTCAGTACC AGTCAAGGTG 
GTACTGCAGC CACAGGCTGG CTCTTCCCAA GGGGCCTCTT CTGGGCTCTC TGTAGTTAAA 
GTTCTGAGTG CCAGTGAAGT GGCAGCTTTG TCATCACCAG CAAGCTCTGC TCCTCATTCG 
GGGGGAAAGA CAGGAATGGA GGAAAACCGC AGATTGGAAC ACCAGAAGAA GCAAGAGAAA 
GCAAATCGGA TTGTAGCAGA GGCCATTGCG AGAGCCCGTG CCCGCGGTGA GCAGAACATA 
CCTCGAGTCT TAAATGAGGA CGAGTTGCCC AGCGTTCGGC CAGAGGAGGA AGGCGAGAAG 
AAACGCAGGA AGAAGAGTGC TGGGGAGAGG CTGAAAGAGG AGAAGCCAAA GAAGAGTAAA 
ACATCTGGTG CCTCCAAAAC AAAGGGCAAG AGCAAGCTCA ACACCATCAC TCCTGTAGTG 
GGTAAGAAGA GAAAACGTAA TACCTCATCT GATAATTCAG ATGTGGAAGT CATGCCTGCA 
CAGTCACCTC GAGAAGATGA AGAAAGCAGC ATTCAGAAGA GACGCTCAAA CCGCCAAGTT 
AAGCGAAAAA AATATACAGA GGACCTGGAT ATAAAGATCA CAGATGATGA AGAAGAAGAA 
GAGGTGGATG TAACTGGTCC AATAAAACCT GAGCCTATCC TCCCTGAACC AGTGCAAGAA 
CCAGATGGCG AGACTTTGCC TTCCATGCAG TTCTTTGTGG AGAATCCCAG TGAAGAAGAT 
GCAGCCATTG TAGACAAAGT GCTTTCTATG CGGATTGTGA AGAAGGAGCT CCCTTCTGGA 
CAATATACTG AAGCAGAAGA ATTCTTTGTC AAGTACAAGA ACTACTCCTA TCTGCATTGT 
GAATGGGCTA CTATCTCCCA ACTAGAGAAG GATAAGAGGA TACATCAAAA ATTAAAGCGC 
TTCAAAACCA AAATGGCTCA GATGAGACAC TTCTTCCATG AGGATGAAGA GCCCTTTAAT 
CCAGACTACG TAGAGGTGGA TAGGATATTG GATGAGTCTC ACAGTATTGA CAAGGACAAT 
GGGGAGCCCG TTATTTACTA CCTGGTGAAA TGGTGCTCTC TGCCCTATGA GGATAGCACA 
TGGGAGCTAA AAGAAGATGT TGATGAGGGC AAGATTCGAG AATTTAAACG GATTCAGTCA 
AGGCACCCAG AACTCAAAAG GGTGAATCGT CCGCAGGCAA GTGCCTGGAA GAAATTGGAG 
CTATCACATG AATATAAAAA CAGAAACCAG CTACGGGAAT ATCAGTTGGA AGGGGTTAAT 
TGGCTGCTCT TTAATTGGTA TAACAGGCAG AACTGCATCC TGGCTGATGA GATGGGATTG 
GGCAAAACTA TTCAGTCCAT TGCCTTCTTG CAGGAAGTAT ATAATGTGGG CATCCATGGT 
CCCTTCTTGG TCATTGCCCC ACTGTCCACA ATTACTAACT GGGAGCGAGA ATTTAATACA 
TGGACAGAAA TGAACACTAT TGTGTACCAT GGCAGTCTGG CCAGCAGGCA GATGATTCAA 
CAGTATGAAA TGTACTGCAA AGATTCACGG GGACGCCTCA TCCCAGGCGC ATACAAGTTT 
GACGCTCTGA TCACCACTTT TGAGATGATT TTGTCAGATT GTCCTGAGCT TCGTGAAATT 
GAATGGCGTT GTGTTATCAT TGATGAAGCC CATCGACTGA AAAACCGTAA TTGCAAGCTG 
CTTGATAGTC TCAAGCACAT GGACCTGGAA CACAAGGTGC TACTCACAGG AACACCATTG 
CAAAATACTG TAGAAGAACT GTTTAGCTTG CTTCATTTCT TGGAACCGTC ACAATTTCCC 
TCAGAATCAG AGTTTCTCAA GGACTTTGGG GATCTCAAGA CAGAGGAACA GGTTCAAAAG 
CTACAGGCCA TTCTTAAGCC AATGATGCTG AGAAGACTCA AAGAGGATGT TGAAAAAAAC 
TTGGCACCCA AACAGGAAAC AATTATTGAA GTAGAGCTGA CTAATATCCA GAAGAAATAC 
TATCGGGCTA TTTTGGAGAA GAATTTCTCC TTCCTTTCCA AAGGGGCAGG TCATACCAAC 
ATGCCTAATC TACTTAACAC AATGATGGAG TTGCGCAAGT GCTGCAACCA CCCATATCTC 
ATCAATGGTG CTGAAGAAAA AATCCTAACA GAATTCCGTG AAGCTTGCCA TATTATACCT 
CATGACTTTC ACCTGCAGGC CATGGTTCGT TCAGCCGGCA AACTGGTTCT TATTGACAAG 
TTGCTTCCAA AGCTTAAAGC TGGTGGCCAT AAAGTTCTGA TCTTCTCTCA GATGGTGCGC 
TGCCTAGACA TCCTAGAGGA TTATTTAATC CAGAGGAGGT ACTTATATGA ACGTATTGAT 
GGGCGAGTTA GAGGCAACCT TCGACAGGCT GCCATTGACC GCTTCAGCAA GCCTGACTCA 
GACCGCTTTG TCTTCTTACT GTGTACCCGG GCTGGTGGAC TTGGTATTAA TCTTACAGCT 
GCTGATACCT GCATCATCTT TGATTCAGAC TGGAATCCAC AAAATGACCT GCAGGCCCAG 
GCACGATGTC ATCGAATTGG GCAGAGCAAA GCTGTGAAGG TGTACCGCCT CATCACTCGT 
AATTCCTACG AGAGAGAGAT GTTTGATAAG GCCAGCCTCA AGTTGGGGTT GGATAAGGCT 
GTGCTTCAAT CCATGAGTGG TCGGGATGGC AACATTACTG GAATCCAACA GTTCTCTAAG 
AAGGAGATTG AAGATCTTTT AAGAAAAGGA GCATATGCAG CCATCATGGA GGAAGATGAT 
GAAGGCTCCA AGTTTTGTGA AGAGGACATT GACCAGATCT TGTTAAGACG AACTACAACC 
ATCACCATTG AATCTGAAGG AAAAGGTTCC ACCTTTGCTA AGGCAAGCTT TGTTGCTTCT 
GAAAACAGGA CAGATATTTC TTTGGATGAC CCCAACTTTT GGCAAAAGTG GGCCAAAAAG 
GCTGACCTAG ACATGGATCT GCTCAACAGC AAGAATAATT TGGTAATTGA CACACCTAGA 
GTACGAAAAC AAACGCGCCA CTTTAGCACT CTGAAAGATG ATGACCTGGT GGAATTCTCT 
GATTTGGAAA GTGAGGATGA TGAGCGGCCA CGCTCCCGCA GACATGACCG TCATCATGCC 
TATGGGCGCA CTGACTGCTT TCGGGTGGAA AAGCATCTCC TGGTATATGG TTGGGGACGA 
TGGCGAGATA TTTTATCTCA TGGACGCTTC AAGCGACGTA TGACTGAACG AGATGTGGAG 
ACCATTTGTC GGGCCATTCT CGTGTACTGT CTTCTACACT ACCGTGGGGA TGAAAATATT 
AAAGGCTTCA TCTGGGACTT GATTAGCCCA GCTGAAAATG GCAAGACAAA AGAATTGCAG 
AATCATTCAG GTCTATCTAT CCCTGTGCCT CGTGGACGCA AAGGAAAAAA AGTAAAGTCA 
CAAAGCACTT TTGATATCCA TAAGGCAGAT TGGATCCGGA AATATAACCC TGACACTTTG 
TTCCAAGATG AAAGTTATAA GAAGCACTTG AAACATCAGT GTAACAAGGT ACTGTTGCGG 
GTACGAATGC TATACTACCT GAGGCAGGAG GTTATTGGAG ACCAAGCAGA AAAGGTGTTA 
GGGGGTGCGA TTGCCAGTGA GATTGACATA TGGTTCCCAG TAGTGGATCA ACTGGAGGTT 
CCAACAACTT GGTGGGACAG TGAGGCTGAC AAGTCGCTGC TCATTGGAGT CTTTAAACAT 
GGCTATGAGA AATATAATAC CATGAGGGCA GACCCAGCCT TATGTTTCCT AGAAAAGGCT 
GGCCGACCAG ATGACAAAGC AATTGCAGCA GAACATCGAG TGTTGGATAA CTTCTCTGAC 
ATAGTAGAAG GGGTTGACTT TGATAAAGAT TGTGAAGATC CTGAATATAA ACCACTCCAA 
GGTCCCCCAA AGGACCAAGA TGATGAGGGT GATCCCTTGA TGATGATGGA TGAGGAGATC 
TCAGTGATTG ATGGAGATGA AGCCCAGGTG ACCCAACAGC CAGGCCATTT ATTCTGGCCT 
CCGGGCTCTG CCCTAACAGC TAGGCTTCGG CGTCTAGTAA CAGCGTATCA GCGCAGCTAC 
AAGAGAGAAC AAATGAAGAT AGAGGCTGCA GAACGTGGGG ACCGGCGAAG GCGGCGTTGT 
GAAGCAGCCT TCAAGCTGAA AGAAATTGCA CGGCGGGAGA AACAACAACG ATGGACAAGG 
CGTGAACAAA CTGATTTTTA TCGAGTGGTG TCTACGTTTG GTGTGGAATA TGACCCTGAC 
ACCATGCAGT TCCATTGGGA TCGCTTCCGC ACTTTTGCTC GACTAGACAA AAAGACAGAT 
GAAAGCCTTA CCAAGTACTT CCATGGCTTT GTGGCCATGT GCCGCCAAGT ATGCCGCCTT 
CCCCCAGCAG CTGGAGATGA ACCCCCCGAC CCTAACCTGT TCATTGAGCC CATCACTGAG 
GAGAGAGCCT CACGGACTCT CTACCGTATA GAATTGCTTC GGCGCTTACG GGAACAAGTT 
TTATGCCACC CCCTTTTGGA AGATCGGCTG GCATTGTGTC AGCCTCCTGG TCCTGAATTG 
CCCAAATGGT GGGAGCCTGT TCGGCATGAT GGGGAGCTTC TAAGAGGGGC AGCCCGCCAT 
GGGGTGAGCC AAACAGACTG CAACATCATG CAGGACCCAG ACTTCTCTTT TCTGGCTGCC 
CGTATGAATT ATATGCAGAA CCATCAAGCA GGAGCACCAG CTCCATCCTT GTCACGCTGC 
TCTACTCCAC TGCTGCACCA GCAGTATACC TCACGCACTG CCTCACCACT GCCCCTGCGC 
CCAGATGCTC CTGTTGAAAA GTCACCCGAG GAGACAGCTA CCCAGGTCCC CAGTCTGGAG 
AGTCTGACTT TAAAGCTAGA GCACGAGGTG GTGGCCAGGA GCCGACCAAC CCCACAAGAC 
TATGAGATGC GAGTATCCCC CTCTGATACT ACCCCTCTGG TTTCCCGGAG TGTTCCACCA 
GTCAAACTGG AGGATGAGGA TGATTCGGAC TCTGAGCTGG ACTTGAGCAA GCTGTCACCA 
TCTTCTTCTT CTTCCTCATC CTCATCCAGC TCCAGCTCCA GCACTGATGA GAGTGAGGAT 
GAGAAGGAAG AGAAGCTAAC TGACCAGTCC CGCTCAAAGC TCTATGATGA AGAGAGTCTC 
CTGTCCCTCA CTATGTCCCA AGATGGATTC CCAAATGAAG ATGGAGAACA AATGACCCCT 
GAGCTTCTGC TACTGCAGGA AAGACAAAGA GCCTCTGAGT GGCCCAAGGA TCGTGTCCTG 
ATAAACCGTA TTGACCTCGT CTGCCAGGCT GTACTCTCAG GGAAGTGGCC TTCTAGCCGT 
AGGAGCCAGG AAATGGTAAC AGGAGGAATT TTGGGGCCAG GCAACCACTT GCTAGACAGT 
CCCTCATTGA CTCCTGGAGA ATATGGTGAC TCTCCAGTCC CCACACCACG AAGTAGTAGT 
GCAGCTTCCA TGGCAGAGGA GGAAGCATCT GCAGTCAGCA CAGCGGCAGC CCAGTTCACC 
AAACTTCGCC GAGGCATGGA TGAAAAGGAG TTTACAGTTC AAATCAAAGA TGAGGAAGGA 
TTGAAGTTAA CATTCCAGAA GCACAAGTTG ATGGCGAATG GAGTAATGGG AGATGGACAT 
CCACTGTTTC ATAAGAAGAA GGGGAACAGA AAGAAGCTAG TAGAGCTGGA GGTGGAGTGC 
ATGGAAGAGC CTAATCACCT TGATGTGGAC CTGGAGACCC GGATCCCTGT CATCAATAAG 
GTGGATGGTA CTTTGCTGGT GGGTGAGGAT GCCCCTCGCC GGGCTGAACT GGAGATGTGG 
TTACAGGGTC ATCCAGAGTT TGCTGTTGAT CCCCGATTTC TAGCGTATAT GGAGGATCGC 
AGAAAACAGA AGTGGCAAAG ATGTAAAAAA AATAATAAGG CAGAATTGAA CTGTTTGGGA 
ATGGAACCAG TACAGACAGC TAACTCTAGA AATGGGAAAA AGGGTCATCA CACTGAAACG 
GTGTTCAACC GGGTTTTGCC AGGGCCTATT GCACCAGAGA GCAGCAAGAA GCGGGCCCGT 
AGGATGCGAC CAGACCTTTC TAAGATGATG GCCCTCATGC AGGGTGGAAG CACTGGGTCT 
CTATCTCTGC ATAACACGTT CCAACACAGC AGTAGTGGCC TACAGTCTGT GTCATCTTTG 
GGTCACAGCA GTGCCACTTC TGCATCTTTG CCTTTTATGC CATTTGTGAT GGGTGGTGCA 
CCATCATCCC CTCATGTAGA CTCCAGCACC ATGCTTCATC ACCACCACCA CCACCCCCAC 
CCCCACCATC ACCACCATCA CCATCCAGGC TTGAGAGCCC CTGGCTACCC CTCTTCACCA 
GTGACTACCG CCTCTGGTAC TACCTTGCGG TTGCCACCAC TGCAACCTGA GGAGGATGAC 
GATGAGGATG AAGAAGATGA TGATGACTTA TCTCAGGGCT ATGATAGCTC AGAAAGGGAC 
TTCTCACTCA TTGATGATCC TATGATGCCA GCTAACTCAG ACTCCAGTGA AGATGCTGAT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164100.1
CDS	66..7811
Translation	MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASELVPPPEETAPTELSKESTAPAPESITLHDYTTQPASQEQPAQPVLQTSTPTSGLLQVSKSQEILSQGNPFMGVSATAVSSSSAGGQPPQSAPKIVILKAPPSSSVTGAHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLAAKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGATGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQQPPSTQPVTLSSVQQAQIMGPGQSPGQRLSVPVKVVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASSAPHSGGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSAGERLKEEKPKKSKTSGASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRIVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSIDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELKRVNRPQASAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILTEFREACHIIPHDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHAYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKGFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDIVEGVDFDKDCEDPEYKPLQGPPKDQDDEGDPLMMMDEEISVIDGDEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKIEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDTMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGPELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGAPAPSLSRCSTPLLHQQYTSRTASPLPLRPDAPVEKSPEETATQVPSLESLTLKLEHEVVARSRPTPQDYEMRVSPSDTTPLVSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTDQSRSKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSSRRSQEMVTGGILGPGNHLLDSPSLTPGEYGDSPVPTPRSSSAASMAEEEASAVSTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHKLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDVDLETRIPVINKVDGTLLVGEDAPRRAELEMWLQGHPEFAVDPRFLAYMEDRRKQKWQRCKKNNKAELNCLGMEPVQTANSRNGKKGHHTETVFNRVLPGPIAPESSKKRARRMRPDLSKMMALMQGGSTGSLSLHNTFQHSSSGLQSVSSLGHSSATSASLPFMPFVMGGAPSSPHVDSSTMLHHHHHHPHPHHHHHHHPGLRAPGYPSSPVTTASGTTLRLPPLQPEEDDDEDEEDDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	NM_001170629.1
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170629.1

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTGTTCGAT GACCCAAATT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAACCA GGTCACACAA GACCCCATTG AGGAAGCCCT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAGGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGAACTGGT CCCTCCACCA GAGGAAACAG CTCCCACAGA ACTTTCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCATA ACCTTGCATG ATTATACCAC TCAGCCTGCC 
AGCCAGGAGC AGCCAGCCCA ACCTGTCTTA CAGACATCGA CGCCAACATC AGGACTTTTG 
CAAGTCTCCA AGAGCCAGGA GATCCTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGCTGTCT CCTCCAGTAG TGCTGGAGGG CAGCCACCTC AGTCAGCCCC TAAGATTGTT 
ATCCTTAAGG CCCCACCAAG CTCCTCAGTC ACTGGTGCCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGCACAGC TCAGCCCCTG GTGGCAGGCA CAGCCAATGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTAAC CGGCACACCC CTTCGACCAG GTGTTTCCAT TGTCTCTGGT 
AATACAGTGT TGGCCGCCAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCCAGCCGAC CAGTAAAGCA GCTGGTCCTC CAGCCAGTTA AGGGTTCAGC TCCTGCTGGA 
AACCCTGGGG CCACAGGGCC CCCACTGAAG CCTGCAGTTA CACTGACCTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACCCTGGTC CTCCAGCAGC CACAGTCTGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGCT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTGGCAGCCC TGACTCAAGC CAAGAATGCC CAAGGGCAGC CTGCCAAGGT AGTAACTATC 
CAGCTGCAGG TGCAGCAGCC ACAGCAAAAA ATCCAGATTG TACCACAACC ACCATCATCG 
CAGCCACAGC CCCAGCAGCC ACCCTCCACC CAGCCAGTGA CTCTGTCCTC TGTACAGCAG 
GCTCAGATAA TGGGACCAGG ACAAAGCCCA GGACAAAGAC TTTCAGTACC AGTCAAGGTG 
GTACTGCAGC CACAGGCTGG CTCTTCCCAA GGGGCCTCTT CTGGGCTCTC TGTAGTTAAA 
GTTCTGAGTG CCAGTGAAGT GGCAGCTTTG TCATCACCAG CAAGCTCTGC TCCTCATTCG 
GGGGGAAAGA CAGGAATGGA GGAAAACCGC AGATTGGAAC ACCAGAAGAA GCAAGAGAAA 
GCAAATCGGA TTGTAGCAGA GGCCATTGCG AGAGCCCGTG CCCGCGGTGA GCAGAACATA 
CCTCGAGTCT TAAATGAGGA CGAGTTGCCC AGCGTTCGGC CAGAGGAGGA AGGCGAGAAG 
AAACGCAGGA AGAAGAGTGC TGGGGAGAGG CTGAAAGAGG AGAAGCCAAA GAAGAGTAAA 
ACATCTGGTG CCTCCAAAAC AAAGGGCAAG AGCAAGCTCA ACACCATCAC TCCTGTAGTG 
GGTAAGAAGA GAAAACGTAA TACCTCATCT GATAATTCAG ATGTGGAAGT CATGCCTGCA 
CAGTCACCTC GAGAAGATGA AGAAAGCAGC ATTCAGAAGA GACGCTCAAA CCGCCAAGTT 
AAGCGAAAAA AATATACAGA GGACCTGGAT ATAAAGATCA CAGATGATGA AGAAGAAGAA 
GAGGTGGATG TAACTGGTCC AATAAAACCT GAGCCTATCC TCCCTGAACC AGTGCAAGAA 
CCAGATGGCG AGACTTTGCC TTCCATGCAG TTCTTTGTGG AGAATCCCAG TGAAGAAGAT 
GCAGCCATTG TAGACAAAGT GCTTTCTATG CGGATTGTGA AGAAGGAGCT CCCTTCTGGA 
CAATATACTG AAGCAGAAGA ATTCTTTGTC AAGTACAAGA ACTACTCCTA TCTGCATTGT 
GAATGGGCTA CTATCTCCCA ACTAGAGAAG GATAAGAGGA TACATCAAAA ATTAAAGCGC 
TTCAAAACCA AAATGGCTCA GATGAGACAC TTCTTCCATG AGGATGAAGA GCCCTTTAAT 
CCAGACTACG TAGAGGTGGA TAGGATATTG GATGAGTCTC ACAGTATTGA CAAGGACAAT 
GGGGAGCCCG TTATTTACTA CCTGGTGAAA TGGTGCTCTC TGCCCTATGA GGATAGCACA 
TGGGAGCTAA AAGAAGATGT TGATGAGGGC AAGATTCGAG AATTTAAACG GATTCAGTCA 
AGGCACCCAG AACTCAAAAG GGTGAATCGT CCGCAGGCAA GTGCCTGGAA GAAATTGGAG 
CTATCACATG AATATAAAAA CAGAAACCAG CTACGGGAAT ATCAGTTGGA AGGGGTTAAT 
TGGCTGCTCT TTAATTGGTA TAACAGGCAG AACTGCATCC TGGCTGATGA GATGGGATTG 
GGCAAAACTA TTCAGTCCAT TGCCTTCTTG CAGGAAGTAT ATAATGTGGG CATCCATGGT 
CCCTTCTTGG TCATTGCCCC ACTGTCCACA ATTACTAACT GGGAGCGAGA ATTTAATACA 
TGGACAGAAA TGAACACTAT TGTGTACCAT GGCAGTCTGG CCAGCAGGCA GATGATTCAA 
CAGTATGAAA TGTACTGCAA AGATTCACGG GGACGCCTCA TCCCAGGCGC ATACAAGTTT 
GACGCTCTGA TCACCACTTT TGAGATGATT TTGTCAGATT GTCCTGAGCT TCGTGAAATT 
GAATGGCGTT GTGTTATCAT TGATGAAGCC CATCGACTGA AAAACCGTAA TTGCAAGCTG 
CTTGATAGTC TCAAGCACAT GGACCTGGAA CACAAGGTGC TACTCACAGG AACACCATTG 
CAAAATACTG TAGAAGAACT GTTTAGCTTG CTTCATTTCT TGGAACCGTC ACAATTTCCC 
TCAGAATCAG AGTTTCTCAA GGACTTTGGG GATCTCAAGA CAGAGGAACA GGTTCAAAAG 
CTACAGGCCA TTCTTAAGCC AATGATGCTG AGAAGACTCA AAGAGGATGT TGAAAAAAAC 
TTGGCACCCA AACAGGAAAC AATTATTGAA GTAGAGCTGA CTAATATCCA GAAGAAATAC 
TATCGGGCTA TTTTGGAGAA GAATTTCTCC TTCCTTTCCA AAGGGGCAGG TCATACCAAC 
ATGCCTAATC TACTTAACAC AATGATGGAG TTGCGCAAGT GCTGCAACCA CCCATATCTC 
ATCAATGGTG CTGAAGAAAA AATCCTAACA GAATTCCGTG AAGCTTGCCA TATTATACCT 
CATGACTTTC ACCTGCAGGC CATGGTTCGT TCAGCCGGCA AACTGGTTCT TATTGACAAG 
TTGCTTCCAA AGCTTAAAGC TGGTGGCCAT AAAGTTCTGA TCTTCTCTCA GATGGTGCGC 
TGCCTAGACA TCCTAGAGGA TTATTTAATC CAGAGGAGGT ACTTATATGA ACGTATTGAT 
GGGCGAGTTA GAGGCAACCT TCGACAGGCT GCCATTGACC GCTTCAGCAA GCCTGACTCA 
GACCGCTTTG TCTTCTTACT GTGTACCCGG GCTGGTGGAC TTGGTATTAA TCTTACAGCT 
GCTGATACCT GCATCATCTT TGATTCAGAC TGGAATCCAC AAAATGACCT GCAGGCCCAG 
GCACGATGTC ATCGAATTGG GCAGAGCAAA GCTGTGAAGG TGTACCGCCT CATCACTCGT 
AATTCCTACG AGAGAGAGAT GTTTGATAAG GCCAGCCTCA AGTTGGGGTT GGATAAGGCT 
GTGCTTCAAT CCATGAGTGG TCGGGATGGC AACATTACTG GAATCCAACA GTTCTCTAAG 
AAGGAGATTG AAGATCTTTT AAGAAAAGGA GCATATGCAG CCATCATGGA GGAAGATGAT 
GAAGGCTCCA AGTTTTGTGA AGAGGACATT GACCAGATCT TGTTAAGACG AACTACAACC 
ATCACCATTG AATCTGAAGG AAAAGGTTCC ACCTTTGCTA AGGCAAGCTT TGTTGCTTCT 
GAAAACAGGA CAGATATTTC TTTGGATGAC CCCAACTTTT GGCAAAAGTG GGCCAAAAAG 
GCTGACCTAG ACATGGATCT GCTCAACAGC AAGAATAATT TGGTAATTGA CACACCTAGA 
GTACGAAAAC AAACGCGCCA CTTTAGCACT CTGAAAGATG ATGACCTGGT GGAATTCTCT 
GATTTGGAAA GTGAGGATGA TGAGCGGCCA CGCTCCCGCA GACATGACCG TCATCATGCC 
TATGGGCGCA CTGACTGCTT TCGGGTGGAA AAGCATCTCC TGGTATATGG TTGGGGACGA 
TGGCGAGATA TTTTATCTCA TGGACGCTTC AAGCGACGTA TGACTGAACG AGATGTGGAG 
ACCATTTGTC GGGCCATTCT CGTGTACTGT CTTCTACACT ACCGTGGGGA TGAAAATATT 
AAAGGCTTCA TCTGGGACTT GATTAGCCCA GCTGAAAATG GCAAGACAAA AGAATTGCAG 
AATCATTCAG GTCTATCTAT CCCTGTGCCT CGTGGACGCA AAGGAAAAAA AGTAAAGTCA 
CAAAGCACTT TTGATATCCA TAAGGCAGAT TGGATCCGGA AATATAACCC TGACACTTTG 
TTCCAAGATG AAAGTTATAA GAAGCACTTG AAACATCAGT GTAACAAGGT ACTGTTGCGG 
GTACGAATGC TATACTACCT GAGGCAGGAG GTTATTGGAG ACCAAGCAGA AAAGGTGTTA 
GGGGGTGCGA TTGCCAGTGA GATTGACATA TGGTTCCCAG TAGTGGATCA ACTGGAGGTT 
CCAACAACTT GGTGGGACAG TGAGGCTGAC AAGTCGCTGC TCATTGGAGT CTTTAAACAT 
GGCTATGAGA AATATAATAC CATGAGGGCA GACCCAGCCT TATGTTTCCT AGAAAAGGCT 
GGCCGACCAG ATGACAAAGC AATTGCAGCA GAACATCGAG TGTTGGATAA CTTCTCTGAC 
ATAGTAGAAG GGGTTGACTT TGATAAAGAT TGTGAAGATC CTGAATATAA ACCACTCCAA 
GGTCCCCCAA AGGACCAAGA TGATGAGGGT GATCCCTTGA TGATGATGGA TGAGGAGATC 
TCAGTGATTG ATGGAGATGA AGCCCAGGTG ACCCAACAGC CAGGCCATTT ATTCTGGCCT 
CCGGGCTCTG CCCTAACAGC TAGGCTTCGG CGTCTAGTAA CAGCGTATCA GCGCAGCTAC 
AAGAGAGAAC AAATGAAGAT AGAGGCTGCA GAACGTGGGG ACCGGCGAAG GCGGCGTTGT 
GAAGCAGCCT TCAAGCTGAA AGAAATTGCA CGGCGGGAGA AACAACAACG ATGGACAAGG 
CGTGAACAAA CTGATTTTTA TCGAGTGGTG TCTACGTTTG GTGTGGAATA TGACCCTGAC 
ACCATGCAGT TCCATTGGGA TCGCTTCCGC ACTTTTGCTC GACTAGACAA AAAGACAGAT 
GAAAGCCTTA CCAAGTACTT CCATGGCTTT GTGGCCATGT GCCGCCAAGT ATGCCGCCTT 
CCCCCAGCAG CTGGAGATGA ACCCCCCGAC CCTAACCTGT TCATTGAGCC CATCACTGAG 
GAGAGAGCCT CACGGACTCT CTACCGTATA GAATTGCTTC GGCGCTTACG GGAACAAGTT 
TTATGCCACC CCCTTTTGGA AGATCGGCTG GCATTGTGTC AGCCTCCTGG TCCTGAATTG 
CCCAAATGGT GGGAGCCTGT TCGGCATGAT GGGGAGCTTC TAAGAGGGGC AGCCCGCCAT 
GGGGTGAGCC AAACAGACTG CAACATCATG CAGGACCCAG ACTTCTCTTT TCTGGCTGCC 
CGTATGAATT ATATGCAGAA CCATCAAGCA GGAGCACCAG CTCCATCCTT GTCACGCTGC 
TCTACTCCAC TGCTGCACCA GCAGTATACC TCACGCACTG CCTCACCACT GCCCCTGCGC 
CCAGATGCTC CTGTTGAAAA GTCACCCGAG GAGACAGCTA CCCAGGTCCC CAGTCTGGAG 
AGTCTGACTT TAAAGCTAGA GCACGAGGTG GTGGCCAGGA GCCGACCAAC CCCACAAGAC 
TATGAGATGC GAGTATCCCC CTCTGATACT ACCCCTCTGG TTTCCCGGAG TGTTCCACCA 
GTCAAACTGG AGGATGAGGA TGATTCGGAC TCTGAGCTGG ACTTGAGCAA GCTGTCACCA 
TCTTCTTCTT CTTCCTCATC CTCATCCAGC TCCAGCTCCA GCACTGATGA GAGTGAGGAT 
GAGAAGGAAG AGAAGCTAAC TGACCAGTCC CGCTCAAAGC TCTATGATGA AGAGAGTCTC 
CTGTCCCTCA CTATGTCCCA AGATGGATTC CCAAATGAAG ATGGAGAACA AATGACCCCT 
GAGCTTCTGC TACTGCAGGA AAGACAAAGA GCCTCTGAGT GGCCCAAGGA TCGTGTCCTG 
ATAAACCGTA TTGACCTCGT CTGCCAGGCT GTACTCTCAG GGAAGTGGCC TTCTAGCCGT 
AGGAGCCAGG AAATGGTAAC AGGAGGAATT TTGGGGCCAG GCAACCACTT GCTAGACAGT 
CCCTCATTGA CTCCTGGAGA ATATGGTGAC TCTCCAGTCC CCACACCACG AAGTAGTAGT 
GCAGCTTCCA TGGCAGAGGA GGAAGCATCT GCAGTCAGCA CAGCGGCAGC CCAGTTCACC 
AAACTTCGCC GAGGCATGGA TGAAAAGGAG TTTACAGTTC AAATCAAAGA TGAGGAAGGA 
TTGAAGTTAA CATTCCAGAA GCACAAGTTG ATGGCGAATG GAGTAATGGG AGATGGACAT 
CCACTGTTTC ATAAGAAGAA GGGGAACAGA AAGAAGCTAG TAGAGCTGGA GGTGGAGTGC 
ATGGAAGAGC CTAATCACCT TGATGTGGAC CTGGAGACCC GGATCCCTGT CATCAATAAG 
GTGGATGGTA CTTTGCTGGT GGGTGAGGAT GCCCCTCGCC GGGCTGAACT GGAGATGTGG 
TTACAGGGTC ATCCAGAGTT TGCTGTTGAT CCCCGATTTC TAGCGTATAT GGAGGATCGC 
AGAAAACAGA AGTGGCAAAG ATGTAAAAAA AATAATAAGG CAGAATTGAA CTGTTTGGGA 
ATGGAACCAG TACAGACAGC TAACTCTAGA AATGGGAAAA AGGGTCATCA CACTGAAACG 
GTGTTCAACC GGGTTTTGCC AGGGCCTATT GCACCAGAGA GCAGCAAGAA GCGGGCCCGT 
AGGATGCGAC CAGACCTTTC TAAGATGATG GCCCTCATGC AGGGTGGAAG CACTGGGTCT 
CTATCTCTGC ATAACACGTT CCAACACAGC AGTAGTGGCC TACAGTCTGT GTCATCTTTG 
GGTCACAGCA GTGCCACTTC TGCATCTTTG CCTTTTATGC CATTTGTGAT GGGTGGTGCA 
CCATCATCCC CTCATGTAGA CTCCAGCACC ATGCTTCATC ACCACCACCA CCACCCCCAC 
CCCCACCATC ACCACCATCA CCATCCAGGC TTGAGAGCCC CTGGCTACCC CTCTTCACCA 
GTGACTACCG CCTCTGGTAC TACCTTGCGG TTGCCACCAC TGCAACCTGA GGAGGATGAC 
GATGAGGATG AAGAAGATGA TGATGACTTA TCTCAGGGCT ATGATAGCTC AGAAAGGGAC 
TTCTCACTCA TTGATGATCC TATGATGCCA GCTAACTCAG ACTCCAGTGA AGATGCTGAT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD8 ( NM_001170629.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001164100.1 Homo sapiens chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 1, mRNA.

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CloneID	OHu15118
Clone ID Related Accession (Same CDS sequence)	NM_001170629.1 , NM_001170629.2
Accession Version	NM_001170629.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7746bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-28
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 8 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL161747.5, AB046784.3, AK122910.1 and BC036920.1. On Jul 1, 2019 this sequence version replaced NM_001170629.1. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript and protein alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## inferred exon combination :: based on alignments, homology RefSeq Select criteria :: based on manual assertion, conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCAGACC CCATCATGGA TCTGTTCGAT GACCCAAATT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAACCA GGTCACACAA GACCCCATTG AGGAAGCCCT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAGGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGAACTGGT CCCTCCACCA GAGGAAACAG CTCCCACAGA ACTTTCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCATA ACCTTGCATG ATTATACCAC TCAGCCTGCC 
AGCCAGGAGC AGCCAGCCCA ACCTGTCTTA CAGACATCGA CGCCAACATC AGGACTTTTG 
CAAGTCTCCA AGAGCCAGGA GATCCTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGCTGTCT CCTCCAGTAG TGCTGGAGGG CAGCCACCTC AGTCAGCCCC TAAGATTGTT 
ATCCTTAAGG CCCCACCAAG CTCCTCAGTC ACTGGTGCCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGCACAGC TCAGCCCCTG GTGGCAGGCA CAGCCAATGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTAAC CGGCACACCC CTTCGACCAG GTGTTTCCAT TGTCTCTGGT 
AATACAGTGT TGGCCGCCAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCCAGCCGAC CAGTAAAGCA GCTGGTCCTC CAGCCAGTTA AGGGTTCAGC TCCTGCTGGA 
AACCCTGGGG CCACAGGGCC CCCACTGAAG CCTGCAGTTA CACTGACCTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACCCTGGTC CTCCAGCAGC CACAGTCTGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGCT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTGGCAGCCC TGACTCAAGC CAAGAATGCC CAAGGGCAGC CTGCCAAGGT AGTAACTATC 
CAGCTGCAGG TGCAGCAGCC ACAGCAAAAA ATCCAGATTG TACCACAACC ACCATCATCG 
CAGCCACAGC CCCAGCAGCC ACCCTCCACC CAGCCAGTGA CTCTGTCCTC TGTACAGCAG 
GCTCAGATAA TGGGACCAGG ACAAAGCCCA GGACAAAGAC TTTCAGTACC AGTCAAGGTG 
GTACTGCAGC CACAGGCTGG CTCTTCCCAA GGGGCCTCTT CTGGGCTCTC TGTAGTTAAA 
GTTCTGAGTG CCAGTGAAGT GGCAGCTTTG TCATCACCAG CAAGCTCTGC TCCTCATTCG 
GGGGGAAAGA CAGGAATGGA GGAAAACCGC AGATTGGAAC ACCAGAAGAA GCAAGAGAAA 
GCAAATCGGA TTGTAGCAGA GGCCATTGCG AGAGCCCGTG CCCGCGGTGA GCAGAACATA 
CCTCGAGTCT TAAATGAGGA CGAGTTGCCC AGCGTTCGGC CAGAGGAGGA AGGCGAGAAG 
AAACGCAGGA AGAAGAGTGC TGGGGAGAGG CTGAAAGAGG AGAAGCCAAA GAAGAGTAAA 
ACATCTGGTG CCTCCAAAAC AAAGGGCAAG AGCAAGCTCA ACACCATCAC TCCTGTAGTG 
GGTAAGAAGA GAAAACGTAA TACCTCATCT GATAATTCAG ATGTGGAAGT CATGCCTGCA 
CAGTCACCTC GAGAAGATGA AGAAAGCAGC ATTCAGAAGA GACGCTCAAA CCGCCAAGTT 
AAGCGAAAAA AATATACAGA GGACCTGGAT ATAAAGATCA CAGATGATGA AGAAGAAGAA 
GAGGTGGATG TAACTGGTCC AATAAAACCT GAGCCTATCC TCCCTGAACC AGTGCAAGAA 
CCAGATGGCG AGACTTTGCC TTCCATGCAG TTCTTTGTGG AGAATCCCAG TGAAGAAGAT 
GCAGCCATTG TAGACAAAGT GCTTTCTATG CGGATTGTGA AGAAGGAGCT CCCTTCTGGA 
CAATATACTG AAGCAGAAGA ATTCTTTGTC AAGTACAAGA ACTACTCCTA TCTGCATTGT 
GAATGGGCTA CTATCTCCCA ACTAGAGAAG GATAAGAGGA TACATCAAAA ATTAAAGCGC 
TTCAAAACCA AAATGGCTCA GATGAGACAC TTCTTCCATG AGGATGAAGA GCCCTTTAAT 
CCAGACTACG TAGAGGTGGA TAGGATATTG GATGAGTCTC ACAGTATTGA CAAGGACAAT 
GGGGAGCCCG TTATTTACTA CCTGGTGAAA TGGTGCTCTC TGCCCTATGA GGATAGCACA 
TGGGAGCTAA AAGAAGATGT TGATGAGGGC AAGATTCGAG AATTTAAACG GATTCAGTCA 
AGGCACCCAG AACTCAAAAG GGTGAATCGT CCGCAGGCAA GTGCCTGGAA GAAATTGGAG 
CTATCACATG AATATAAAAA CAGAAACCAG CTACGGGAAT ATCAGTTGGA AGGGGTTAAT 
TGGCTGCTCT TTAATTGGTA TAACAGGCAG AACTGCATCC TGGCTGATGA GATGGGATTG 
GGCAAAACTA TTCAGTCCAT TGCCTTCTTG CAGGAAGTAT ATAATGTGGG CATCCATGGT 
CCCTTCTTGG TCATTGCCCC ACTGTCCACA ATTACTAACT GGGAGCGAGA ATTTAATACA 
TGGACAGAAA TGAACACTAT TGTGTACCAT GGCAGTCTGG CCAGCAGGCA GATGATTCAA 
CAGTATGAAA TGTACTGCAA AGATTCACGG GGACGCCTCA TCCCAGGCGC ATACAAGTTT 
GACGCTCTGA TCACCACTTT TGAGATGATT TTGTCAGATT GTCCTGAGCT TCGTGAAATT 
GAATGGCGTT GTGTTATCAT TGATGAAGCC CATCGACTGA AAAACCGTAA TTGCAAGCTG 
CTTGATAGTC TCAAGCACAT GGACCTGGAA CACAAGGTGC TACTCACAGG AACACCATTG 
CAAAATACTG TAGAAGAACT GTTTAGCTTG CTTCATTTCT TGGAACCGTC ACAATTTCCC 
TCAGAATCAG AGTTTCTCAA GGACTTTGGG GATCTCAAGA CAGAGGAACA GGTTCAAAAG 
CTACAGGCCA TTCTTAAGCC AATGATGCTG AGAAGACTCA AAGAGGATGT TGAAAAAAAC 
TTGGCACCCA AACAGGAAAC AATTATTGAA GTAGAGCTGA CTAATATCCA GAAGAAATAC 
TATCGGGCTA TTTTGGAGAA GAATTTCTCC TTCCTTTCCA AAGGGGCAGG TCATACCAAC 
ATGCCTAATC TACTTAACAC AATGATGGAG TTGCGCAAGT GCTGCAACCA CCCATATCTC 
ATCAATGGTG CTGAAGAAAA AATCCTAACA GAATTCCGTG AAGCTTGCCA TATTATACCT 
CATGACTTTC ACCTGCAGGC CATGGTTCGT TCAGCCGGCA AACTGGTTCT TATTGACAAG 
TTGCTTCCAA AGCTTAAAGC TGGTGGCCAT AAAGTTCTGA TCTTCTCTCA GATGGTGCGC 
TGCCTAGACA TCCTAGAGGA TTATTTAATC CAGAGGAGGT ACTTATATGA ACGTATTGAT 
GGGCGAGTTA GAGGCAACCT TCGACAGGCT GCCATTGACC GCTTCAGCAA GCCTGACTCA 
GACCGCTTTG TCTTCTTACT GTGTACCCGG GCTGGTGGAC TTGGTATTAA TCTTACAGCT 
GCTGATACCT GCATCATCTT TGATTCAGAC TGGAATCCAC AAAATGACCT GCAGGCCCAG 
GCACGATGTC ATCGAATTGG GCAGAGCAAA GCTGTGAAGG TGTACCGCCT CATCACTCGT 
AATTCCTACG AGAGAGAGAT GTTTGATAAG GCCAGCCTCA AGTTGGGGTT GGATAAGGCT 
GTGCTTCAAT CCATGAGTGG TCGGGATGGC AACATTACTG GAATCCAACA GTTCTCTAAG 
AAGGAGATTG AAGATCTTTT AAGAAAAGGA GCATATGCAG CCATCATGGA GGAAGATGAT 
GAAGGCTCCA AGTTTTGTGA AGAGGACATT GACCAGATCT TGTTAAGACG AACTACAACC 
ATCACCATTG AATCTGAAGG AAAAGGTTCC ACCTTTGCTA AGGCAAGCTT TGTTGCTTCT 
GAAAACAGGA CAGATATTTC TTTGGATGAC CCCAACTTTT GGCAAAAGTG GGCCAAAAAG 
GCTGACCTAG ACATGGATCT GCTCAACAGC AAGAATAATT TGGTAATTGA CACACCTAGA 
GTACGAAAAC AAACGCGCCA CTTTAGCACT CTGAAAGATG ATGACCTGGT GGAATTCTCT 
GATTTGGAAA GTGAGGATGA TGAGCGGCCA CGCTCCCGCA GACATGACCG TCATCATGCC 
TATGGGCGCA CTGACTGCTT TCGGGTGGAA AAGCATCTCC TGGTATATGG TTGGGGACGA 
TGGCGAGATA TTTTATCTCA TGGACGCTTC AAGCGACGTA TGACTGAACG AGATGTGGAG 
ACCATTTGTC GGGCCATTCT CGTGTACTGT CTTCTACACT ACCGTGGGGA TGAAAATATT 
AAAGGCTTCA TCTGGGACTT GATTAGCCCA GCTGAAAATG GCAAGACAAA AGAATTGCAG 
AATCATTCAG GTCTATCTAT CCCTGTGCCT CGTGGACGCA AAGGAAAAAA AGTAAAGTCA 
CAAAGCACTT TTGATATCCA TAAGGCAGAT TGGATCCGGA AATATAACCC TGACACTTTG 
TTCCAAGATG AAAGTTATAA GAAGCACTTG AAACATCAGT GTAACAAGGT ACTGTTGCGG 
GTACGAATGC TATACTACCT GAGGCAGGAG GTTATTGGAG ACCAAGCAGA AAAGGTGTTA 
GGGGGTGCGA TTGCCAGTGA GATTGACATA TGGTTCCCAG TAGTGGATCA ACTGGAGGTT 
CCAACAACTT GGTGGGACAG TGAGGCTGAC AAGTCGCTGC TCATTGGAGT CTTTAAACAT 
GGCTATGAGA AATATAATAC CATGAGGGCA GACCCAGCCT TATGTTTCCT AGAAAAGGCT 
GGCCGACCAG ATGACAAAGC AATTGCAGCA GAACATCGAG TGTTGGATAA CTTCTCTGAC 
ATAGTAGAAG GGGTTGACTT TGATAAAGAT TGTGAAGATC CTGAATATAA ACCACTCCAA 
GGTCCCCCAA AGGACCAAGA TGATGAGGGT GATCCCTTGA TGATGATGGA TGAGGAGATC 
TCAGTGATTG ATGGAGATGA AGCCCAGGTG ACCCAACAGC CAGGCCATTT ATTCTGGCCT 
CCGGGCTCTG CCCTAACAGC TAGGCTTCGG CGTCTAGTAA CAGCGTATCA GCGCAGCTAC 
AAGAGAGAAC AAATGAAGAT AGAGGCTGCA GAACGTGGGG ACCGGCGAAG GCGGCGTTGT 
GAAGCAGCCT TCAAGCTGAA AGAAATTGCA CGGCGGGAGA AACAACAACG ATGGACAAGG 
CGTGAACAAA CTGATTTTTA TCGAGTGGTG TCTACGTTTG GTGTGGAATA TGACCCTGAC 
ACCATGCAGT TCCATTGGGA TCGCTTCCGC ACTTTTGCTC GACTAGACAA AAAGACAGAT 
GAAAGCCTTA CCAAGTACTT CCATGGCTTT GTGGCCATGT GCCGCCAAGT ATGCCGCCTT 
CCCCCAGCAG CTGGAGATGA ACCCCCCGAC CCTAACCTGT TCATTGAGCC CATCACTGAG 
GAGAGAGCCT CACGGACTCT CTACCGTATA GAATTGCTTC GGCGCTTACG GGAACAAGTT 
TTATGCCACC CCCTTTTGGA AGATCGGCTG GCATTGTGTC AGCCTCCTGG TCCTGAATTG 
CCCAAATGGT GGGAGCCTGT TCGGCATGAT GGGGAGCTTC TAAGAGGGGC AGCCCGCCAT 
GGGGTGAGCC AAACAGACTG CAACATCATG CAGGACCCAG ACTTCTCTTT TCTGGCTGCC 
CGTATGAATT ATATGCAGAA CCATCAAGCA GGAGCACCAG CTCCATCCTT GTCACGCTGC 
TCTACTCCAC TGCTGCACCA GCAGTATACC TCACGCACTG CCTCACCACT GCCCCTGCGC 
CCAGATGCTC CTGTTGAAAA GTCACCCGAG GAGACAGCTA CCCAGGTCCC CAGTCTGGAG 
AGTCTGACTT TAAAGCTAGA GCACGAGGTG GTGGCCAGGA GCCGACCAAC CCCACAAGAC 
TATGAGATGC GAGTATCCCC CTCTGATACT ACCCCTCTGG TTTCCCGGAG TGTTCCACCA 
GTCAAACTGG AGGATGAGGA TGATTCGGAC TCTGAGCTGG ACTTGAGCAA GCTGTCACCA 
TCTTCTTCTT CTTCCTCATC CTCATCCAGC TCCAGCTCCA GCACTGATGA GAGTGAGGAT 
GAGAAGGAAG AGAAGCTAAC TGACCAGTCC CGCTCAAAGC TCTATGATGA AGAGAGTCTC 
CTGTCCCTCA CTATGTCCCA AGATGGATTC CCAAATGAAG ATGGAGAACA AATGACCCCT 
GAGCTTCTGC TACTGCAGGA AAGACAAAGA GCCTCTGAGT GGCCCAAGGA TCGTGTCCTG 
ATAAACCGTA TTGACCTCGT CTGCCAGGCT GTACTCTCAG GGAAGTGGCC TTCTAGCCGT 
AGGAGCCAGG AAATGGTAAC AGGAGGAATT TTGGGGCCAG GCAACCACTT GCTAGACAGT 
CCCTCATTGA CTCCTGGAGA ATATGGTGAC TCTCCAGTCC CCACACCACG AAGTAGTAGT 
GCAGCTTCCA TGGCAGAGGA GGAAGCATCT GCAGTCAGCA CAGCGGCAGC CCAGTTCACC 
AAACTTCGCC GAGGCATGGA TGAAAAGGAG TTTACAGTTC AAATCAAAGA TGAGGAAGGA 
TTGAAGTTAA CATTCCAGAA GCACAAGTTG ATGGCGAATG GAGTAATGGG AGATGGACAT 
CCACTGTTTC ATAAGAAGAA GGGGAACAGA AAGAAGCTAG TAGAGCTGGA GGTGGAGTGC 
ATGGAAGAGC CTAATCACCT TGATGTGGAC CTGGAGACCC GGATCCCTGT CATCAATAAG 
GTGGATGGTA CTTTGCTGGT GGGTGAGGAT GCCCCTCGCC GGGCTGAACT GGAGATGTGG 
TTACAGGGTC ATCCAGAGTT TGCTGTTGAT CCCCGATTTC TAGCGTATAT GGAGGATCGC 
AGAAAACAGA AGTGGCAAAG ATGTAAAAAA AATAATAAGG CAGAATTGAA CTGTTTGGGA 
ATGGAACCAG TACAGACAGC TAACTCTAGA AATGGGAAAA AGGGTCATCA CACTGAAACG 
GTGTTCAACC GGGTTTTGCC AGGGCCTATT GCACCAGAGA GCAGCAAGAA GCGGGCCCGT 
AGGATGCGAC CAGACCTTTC TAAGATGATG GCCCTCATGC AGGGTGGAAG CACTGGGTCT 
CTATCTCTGC ATAACACGTT CCAACACAGC AGTAGTGGCC TACAGTCTGT GTCATCTTTG 
GGTCACAGCA GTGCCACTTC TGCATCTTTG CCTTTTATGC CATTTGTGAT GGGTGGTGCA 
CCATCATCCC CTCATGTAGA CTCCAGCACC ATGCTTCATC ACCACCACCA CCACCCCCAC 
CCCCACCATC ACCACCATCA CCATCCAGGC TTGAGAGCCC CTGGCTACCC CTCTTCACCA 
GTGACTACCG CCTCTGGTAC TACCTTGCGG TTGCCACCAC TGCAACCTGA GGAGGATGAC 
GATGAGGATG AAGAAGATGA TGATGACTTA TCTCAGGGCT ATGATAGCTC AGAAAGGGAC 
TTCTCACTCA TTGATGATCC TATGATGCCA GCTAACTCAG ACTCCAGTGA AGATGCTGAT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001164100.1
CDS	308..8053
Translation	MADPIMDLFDDPNLFGLDSLTDDSFNQVTQDPIEEALGLPSSLDSLDQMNQDGGGGDVGNSSASELVPPPEETAPTELSKESTAPAPESITLHDYTTQPASQEQPAQPVLQTSTPTSGLLQVSKSQEILSQGNPFMGVSATAVSSSSAGGQPPQSAPKIVILKAPPSSSVTGAHVAQIQAQGITSTAQPLVAGTANGGKVTFTKVLTGTPLRPGVSIVSGNTVLAAKVPGNQAAVQRIVQPSRPVKQLVLQPVKGSAPAGNPGATGPPLKPAVTLTSTPTQGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQQPPSTQPVTLSSVQQAQIMGPGQSPGQRLSVPVKVVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASSAPHSGGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSAGERLKEEKPKKSKTSGASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRIVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSIDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELKRVNRPQASAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILTEFREACHIIPHDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHAYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKGFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDIVEGVDFDKDCEDPEYKPLQGPPKDQDDEGDPLMMMDEEISVIDGDEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKIEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDTMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGPELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGAPAPSLSRCSTPLLHQQYTSRTASPLPLRPDAPVEKSPEETATQVPSLESLTLKLEHEVVARSRPTPQDYEMRVSPSDTTPLVSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTDQSRSKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSSRRSQEMVTGGILGPGNHLLDSPSLTPGEYGDSPVPTPRSSSAASMAEEEASAVSTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHKLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDVDLETRIPVINKVDGTLLVGEDAPRRAELEMWLQGHPEFAVDPRFLAYMEDRRKQKWQRCKKNNKAELNCLGMEPVQTANSRNGKKGHHTETVFNRVLPGPIAPESSKKRARRMRPDLSKMMALMQGGSTGSLSLHNTFQHSSSGLQSVSSLGHSSATSASLPFMPFVMGGAPSSPHVDSSTMLHHHHHHPHPHHHHHHHPGLRAPGYPSSPVTTASGTTLRLPPLQPEEDDDEDEEDDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	NM_001170629.2
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170629.2

ORF Insert Sequence:

ATGGCAGACC CCATCATGGA TCTGTTCGAT GACCCAAATT TATTTGGCCT GGACTCTCTG 
ACTGATGACA GCTTTAACCA GGTCACACAA GACCCCATTG AGGAAGCCCT TGGACTGCCA 
AGCTCTCTGG ACTCCTTGGA TCAGATGAAC CAGGATGGTG GAGGTGGTGA TGTGGGGAAT 
TCATCAGCAA GTGAACTGGT CCCTCCACCA GAGGAAACAG CTCCCACAGA ACTTTCCAAA 
GAATCCACAG CTCCAGCTCC AGAATCCATA ACCTTGCATG ATTATACCAC TCAGCCTGCC 
AGCCAGGAGC AGCCAGCCCA ACCTGTCTTA CAGACATCGA CGCCAACATC AGGACTTTTG 
CAAGTCTCCA AGAGCCAGGA GATCCTGAGC CAAGGGAATC CTTTCATGGG TGTCTCTGCC 
ACAGCTGTCT CCTCCAGTAG TGCTGGAGGG CAGCCACCTC AGTCAGCCCC TAAGATTGTT 
ATCCTTAAGG CCCCACCAAG CTCCTCAGTC ACTGGTGCCC ATGTGGCACA AATTCAGGCC 
CAAGGTATCA CCAGCACAGC TCAGCCCCTG GTGGCAGGCA CAGCCAATGG TGGAAAAGTC 
ACTTTTACCA AAGTGCTAAC CGGCACACCC CTTCGACCAG GTGTTTCCAT TGTCTCTGGT 
AATACAGTGT TGGCCGCCAA GGTCCCTGGG AACCAGGCTG CTGTTCAGCG CATTGTCCAG 
CCCAGCCGAC CAGTAAAGCA GCTGGTCCTC CAGCCAGTTA AGGGTTCAGC TCCTGCTGGA 
AACCCTGGGG CCACAGGGCC CCCACTGAAG CCTGCAGTTA CACTGACCTC TACACCTACC 
CAGGGTGAAT CGAAACGCAT CACCCTGGTC CTCCAGCAGC CACAGTCTGG AGGTCCCCAA 
GGACATCGGC ATGTTGTGCT AGGGAGTCTA CCAGGCAAGA TAGTGTTACA GGGCAACCAG 
CTGGCAGCCC TGACTCAAGC CAAGAATGCC CAAGGGCAGC CTGCCAAGGT AGTAACTATC 
CAGCTGCAGG TGCAGCAGCC ACAGCAAAAA ATCCAGATTG TACCACAACC ACCATCATCG 
CAGCCACAGC CCCAGCAGCC ACCCTCCACC CAGCCAGTGA CTCTGTCCTC TGTACAGCAG 
GCTCAGATAA TGGGACCAGG ACAAAGCCCA GGACAAAGAC TTTCAGTACC AGTCAAGGTG 
GTACTGCAGC CACAGGCTGG CTCTTCCCAA GGGGCCTCTT CTGGGCTCTC TGTAGTTAAA 
GTTCTGAGTG CCAGTGAAGT GGCAGCTTTG TCATCACCAG CAAGCTCTGC TCCTCATTCG 
GGGGGAAAGA CAGGAATGGA GGAAAACCGC AGATTGGAAC ACCAGAAGAA GCAAGAGAAA 
GCAAATCGGA TTGTAGCAGA GGCCATTGCG AGAGCCCGTG CCCGCGGTGA GCAGAACATA 
CCTCGAGTCT TAAATGAGGA CGAGTTGCCC AGCGTTCGGC CAGAGGAGGA AGGCGAGAAG 
AAACGCAGGA AGAAGAGTGC TGGGGAGAGG CTGAAAGAGG AGAAGCCAAA GAAGAGTAAA 
ACATCTGGTG CCTCCAAAAC AAAGGGCAAG AGCAAGCTCA ACACCATCAC TCCTGTAGTG 
GGTAAGAAGA GAAAACGTAA TACCTCATCT GATAATTCAG ATGTGGAAGT CATGCCTGCA 
CAGTCACCTC GAGAAGATGA AGAAAGCAGC ATTCAGAAGA GACGCTCAAA CCGCCAAGTT 
AAGCGAAAAA AATATACAGA GGACCTGGAT ATAAAGATCA CAGATGATGA AGAAGAAGAA 
GAGGTGGATG TAACTGGTCC AATAAAACCT GAGCCTATCC TCCCTGAACC AGTGCAAGAA 
CCAGATGGCG AGACTTTGCC TTCCATGCAG TTCTTTGTGG AGAATCCCAG TGAAGAAGAT 
GCAGCCATTG TAGACAAAGT GCTTTCTATG CGGATTGTGA AGAAGGAGCT CCCTTCTGGA 
CAATATACTG AAGCAGAAGA ATTCTTTGTC AAGTACAAGA ACTACTCCTA TCTGCATTGT 
GAATGGGCTA CTATCTCCCA ACTAGAGAAG GATAAGAGGA TACATCAAAA ATTAAAGCGC 
TTCAAAACCA AAATGGCTCA GATGAGACAC TTCTTCCATG AGGATGAAGA GCCCTTTAAT 
CCAGACTACG TAGAGGTGGA TAGGATATTG GATGAGTCTC ACAGTATTGA CAAGGACAAT 
GGGGAGCCCG TTATTTACTA CCTGGTGAAA TGGTGCTCTC TGCCCTATGA GGATAGCACA 
TGGGAGCTAA AAGAAGATGT TGATGAGGGC AAGATTCGAG AATTTAAACG GATTCAGTCA 
AGGCACCCAG AACTCAAAAG GGTGAATCGT CCGCAGGCAA GTGCCTGGAA GAAATTGGAG 
CTATCACATG AATATAAAAA CAGAAACCAG CTACGGGAAT ATCAGTTGGA AGGGGTTAAT 
TGGCTGCTCT TTAATTGGTA TAACAGGCAG AACTGCATCC TGGCTGATGA GATGGGATTG 
GGCAAAACTA TTCAGTCCAT TGCCTTCTTG CAGGAAGTAT ATAATGTGGG CATCCATGGT 
CCCTTCTTGG TCATTGCCCC ACTGTCCACA ATTACTAACT GGGAGCGAGA ATTTAATACA 
TGGACAGAAA TGAACACTAT TGTGTACCAT GGCAGTCTGG CCAGCAGGCA GATGATTCAA 
CAGTATGAAA TGTACTGCAA AGATTCACGG GGACGCCTCA TCCCAGGCGC ATACAAGTTT 
GACGCTCTGA TCACCACTTT TGAGATGATT TTGTCAGATT GTCCTGAGCT TCGTGAAATT 
GAATGGCGTT GTGTTATCAT TGATGAAGCC CATCGACTGA AAAACCGTAA TTGCAAGCTG 
CTTGATAGTC TCAAGCACAT GGACCTGGAA CACAAGGTGC TACTCACAGG AACACCATTG 
CAAAATACTG TAGAAGAACT GTTTAGCTTG CTTCATTTCT TGGAACCGTC ACAATTTCCC 
TCAGAATCAG AGTTTCTCAA GGACTTTGGG GATCTCAAGA CAGAGGAACA GGTTCAAAAG 
CTACAGGCCA TTCTTAAGCC AATGATGCTG AGAAGACTCA AAGAGGATGT TGAAAAAAAC 
TTGGCACCCA AACAGGAAAC AATTATTGAA GTAGAGCTGA CTAATATCCA GAAGAAATAC 
TATCGGGCTA TTTTGGAGAA GAATTTCTCC TTCCTTTCCA AAGGGGCAGG TCATACCAAC 
ATGCCTAATC TACTTAACAC AATGATGGAG TTGCGCAAGT GCTGCAACCA CCCATATCTC 
ATCAATGGTG CTGAAGAAAA AATCCTAACA GAATTCCGTG AAGCTTGCCA TATTATACCT 
CATGACTTTC ACCTGCAGGC CATGGTTCGT TCAGCCGGCA AACTGGTTCT TATTGACAAG 
TTGCTTCCAA AGCTTAAAGC TGGTGGCCAT AAAGTTCTGA TCTTCTCTCA GATGGTGCGC 
TGCCTAGACA TCCTAGAGGA TTATTTAATC CAGAGGAGGT ACTTATATGA ACGTATTGAT 
GGGCGAGTTA GAGGCAACCT TCGACAGGCT GCCATTGACC GCTTCAGCAA GCCTGACTCA 
GACCGCTTTG TCTTCTTACT GTGTACCCGG GCTGGTGGAC TTGGTATTAA TCTTACAGCT 
GCTGATACCT GCATCATCTT TGATTCAGAC TGGAATCCAC AAAATGACCT GCAGGCCCAG 
GCACGATGTC ATCGAATTGG GCAGAGCAAA GCTGTGAAGG TGTACCGCCT CATCACTCGT 
AATTCCTACG AGAGAGAGAT GTTTGATAAG GCCAGCCTCA AGTTGGGGTT GGATAAGGCT 
GTGCTTCAAT CCATGAGTGG TCGGGATGGC AACATTACTG GAATCCAACA GTTCTCTAAG 
AAGGAGATTG AAGATCTTTT AAGAAAAGGA GCATATGCAG CCATCATGGA GGAAGATGAT 
GAAGGCTCCA AGTTTTGTGA AGAGGACATT GACCAGATCT TGTTAAGACG AACTACAACC 
ATCACCATTG AATCTGAAGG AAAAGGTTCC ACCTTTGCTA AGGCAAGCTT TGTTGCTTCT 
GAAAACAGGA CAGATATTTC TTTGGATGAC CCCAACTTTT GGCAAAAGTG GGCCAAAAAG 
GCTGACCTAG ACATGGATCT GCTCAACAGC AAGAATAATT TGGTAATTGA CACACCTAGA 
GTACGAAAAC AAACGCGCCA CTTTAGCACT CTGAAAGATG ATGACCTGGT GGAATTCTCT 
GATTTGGAAA GTGAGGATGA TGAGCGGCCA CGCTCCCGCA GACATGACCG TCATCATGCC 
TATGGGCGCA CTGACTGCTT TCGGGTGGAA AAGCATCTCC TGGTATATGG TTGGGGACGA 
TGGCGAGATA TTTTATCTCA TGGACGCTTC AAGCGACGTA TGACTGAACG AGATGTGGAG 
ACCATTTGTC GGGCCATTCT CGTGTACTGT CTTCTACACT ACCGTGGGGA TGAAAATATT 
AAAGGCTTCA TCTGGGACTT GATTAGCCCA GCTGAAAATG GCAAGACAAA AGAATTGCAG 
AATCATTCAG GTCTATCTAT CCCTGTGCCT CGTGGACGCA AAGGAAAAAA AGTAAAGTCA 
CAAAGCACTT TTGATATCCA TAAGGCAGAT TGGATCCGGA AATATAACCC TGACACTTTG 
TTCCAAGATG AAAGTTATAA GAAGCACTTG AAACATCAGT GTAACAAGGT ACTGTTGCGG 
GTACGAATGC TATACTACCT GAGGCAGGAG GTTATTGGAG ACCAAGCAGA AAAGGTGTTA 
GGGGGTGCGA TTGCCAGTGA GATTGACATA TGGTTCCCAG TAGTGGATCA ACTGGAGGTT 
CCAACAACTT GGTGGGACAG TGAGGCTGAC AAGTCGCTGC TCATTGGAGT CTTTAAACAT 
GGCTATGAGA AATATAATAC CATGAGGGCA GACCCAGCCT TATGTTTCCT AGAAAAGGCT 
GGCCGACCAG ATGACAAAGC AATTGCAGCA GAACATCGAG TGTTGGATAA CTTCTCTGAC 
ATAGTAGAAG GGGTTGACTT TGATAAAGAT TGTGAAGATC CTGAATATAA ACCACTCCAA 
GGTCCCCCAA AGGACCAAGA TGATGAGGGT GATCCCTTGA TGATGATGGA TGAGGAGATC 
TCAGTGATTG ATGGAGATGA AGCCCAGGTG ACCCAACAGC CAGGCCATTT ATTCTGGCCT 
CCGGGCTCTG CCCTAACAGC TAGGCTTCGG CGTCTAGTAA CAGCGTATCA GCGCAGCTAC 
AAGAGAGAAC AAATGAAGAT AGAGGCTGCA GAACGTGGGG ACCGGCGAAG GCGGCGTTGT 
GAAGCAGCCT TCAAGCTGAA AGAAATTGCA CGGCGGGAGA AACAACAACG ATGGACAAGG 
CGTGAACAAA CTGATTTTTA TCGAGTGGTG TCTACGTTTG GTGTGGAATA TGACCCTGAC 
ACCATGCAGT TCCATTGGGA TCGCTTCCGC ACTTTTGCTC GACTAGACAA AAAGACAGAT 
GAAAGCCTTA CCAAGTACTT CCATGGCTTT GTGGCCATGT GCCGCCAAGT ATGCCGCCTT 
CCCCCAGCAG CTGGAGATGA ACCCCCCGAC CCTAACCTGT TCATTGAGCC CATCACTGAG 
GAGAGAGCCT CACGGACTCT CTACCGTATA GAATTGCTTC GGCGCTTACG GGAACAAGTT 
TTATGCCACC CCCTTTTGGA AGATCGGCTG GCATTGTGTC AGCCTCCTGG TCCTGAATTG 
CCCAAATGGT GGGAGCCTGT TCGGCATGAT GGGGAGCTTC TAAGAGGGGC AGCCCGCCAT 
GGGGTGAGCC AAACAGACTG CAACATCATG CAGGACCCAG ACTTCTCTTT TCTGGCTGCC 
CGTATGAATT ATATGCAGAA CCATCAAGCA GGAGCACCAG CTCCATCCTT GTCACGCTGC 
TCTACTCCAC TGCTGCACCA GCAGTATACC TCACGCACTG CCTCACCACT GCCCCTGCGC 
CCAGATGCTC CTGTTGAAAA GTCACCCGAG GAGACAGCTA CCCAGGTCCC CAGTCTGGAG 
AGTCTGACTT TAAAGCTAGA GCACGAGGTG GTGGCCAGGA GCCGACCAAC CCCACAAGAC 
TATGAGATGC GAGTATCCCC CTCTGATACT ACCCCTCTGG TTTCCCGGAG TGTTCCACCA 
GTCAAACTGG AGGATGAGGA TGATTCGGAC TCTGAGCTGG ACTTGAGCAA GCTGTCACCA 
TCTTCTTCTT CTTCCTCATC CTCATCCAGC TCCAGCTCCA GCACTGATGA GAGTGAGGAT 
GAGAAGGAAG AGAAGCTAAC TGACCAGTCC CGCTCAAAGC TCTATGATGA AGAGAGTCTC 
CTGTCCCTCA CTATGTCCCA AGATGGATTC CCAAATGAAG ATGGAGAACA AATGACCCCT 
GAGCTTCTGC TACTGCAGGA AAGACAAAGA GCCTCTGAGT GGCCCAAGGA TCGTGTCCTG 
ATAAACCGTA TTGACCTCGT CTGCCAGGCT GTACTCTCAG GGAAGTGGCC TTCTAGCCGT 
AGGAGCCAGG AAATGGTAAC AGGAGGAATT TTGGGGCCAG GCAACCACTT GCTAGACAGT 
CCCTCATTGA CTCCTGGAGA ATATGGTGAC TCTCCAGTCC CCACACCACG AAGTAGTAGT 
GCAGCTTCCA TGGCAGAGGA GGAAGCATCT GCAGTCAGCA CAGCGGCAGC CCAGTTCACC 
AAACTTCGCC GAGGCATGGA TGAAAAGGAG TTTACAGTTC AAATCAAAGA TGAGGAAGGA 
TTGAAGTTAA CATTCCAGAA GCACAAGTTG ATGGCGAATG GAGTAATGGG AGATGGACAT 
CCACTGTTTC ATAAGAAGAA GGGGAACAGA AAGAAGCTAG TAGAGCTGGA GGTGGAGTGC 
ATGGAAGAGC CTAATCACCT TGATGTGGAC CTGGAGACCC GGATCCCTGT CATCAATAAG 
GTGGATGGTA CTTTGCTGGT GGGTGAGGAT GCCCCTCGCC GGGCTGAACT GGAGATGTGG 
TTACAGGGTC ATCCAGAGTT TGCTGTTGAT CCCCGATTTC TAGCGTATAT GGAGGATCGC 
AGAAAACAGA AGTGGCAAAG ATGTAAAAAA AATAATAAGG CAGAATTGAA CTGTTTGGGA 
ATGGAACCAG TACAGACAGC TAACTCTAGA AATGGGAAAA AGGGTCATCA CACTGAAACG 
GTGTTCAACC GGGTTTTGCC AGGGCCTATT GCACCAGAGA GCAGCAAGAA GCGGGCCCGT 
AGGATGCGAC CAGACCTTTC TAAGATGATG GCCCTCATGC AGGGTGGAAG CACTGGGTCT 
CTATCTCTGC ATAACACGTT CCAACACAGC AGTAGTGGCC TACAGTCTGT GTCATCTTTG 
GGTCACAGCA GTGCCACTTC TGCATCTTTG CCTTTTATGC CATTTGTGAT GGGTGGTGCA 
CCATCATCCC CTCATGTAGA CTCCAGCACC ATGCTTCATC ACCACCACCA CCACCCCCAC 
CCCCACCATC ACCACCATCA CCATCCAGGC TTGAGAGCCC CTGGCTACCC CTCTTCACCA 
GTGACTACCG CCTCTGGTAC TACCTTGCGG TTGCCACCAC TGCAACCTGA GGAGGATGAC 
GATGAGGATG AAGAAGATGA TGATGACTTA TCTCAGGGCT ATGATAGCTC AGAAAGGGAC 
TTCTCACTCA TTGATGATCC TATGATGCCA GCTAACTCAG ACTCCAGTGA AGATGCTGAT 
GACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD8 ( NM_020920.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065971.2 Homo sapiens chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 2, mRNA.

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CloneID	OHu17867
Clone ID Related Accession (Same CDS sequence)	NM_020920.3 , NM_020920.4
Accession Version	NM_020920.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 8 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN290520.1, AK122910.1, AB046784.3, AL834524.1, BC036920.1 and AW327785.1. This sequence is a reference standard in the RefSeqGene project. On Dec 29, 2009 this sequence version replaced NM_020920.2. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR and has a distinct 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749315.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGGTG AATCGAAACG CATCACCCTG GTCCTCCAGC AGCCACAGTC TGGAGGTCCC 
CAAGGACATC GGCATGTTGT GCTAGGGAGT CTACCAGGCA AGATAGTGTT ACAGGGCAAC 
CAGCTGGCAG CCCTGACTCA AGCCAAGAAT GCCCAAGGGC AGCCTGCCAA GGTAGTAACT 
ATCCAGCTGC AGGTGCAGCA GCCACAGCAA AAAATCCAGA TTGTACCACA ACCACCATCA 
TCGCAGCCAC AGCCCCAGCA GCCACCCTCC ACCCAGCCAG TGACTCTGTC CTCTGTACAG 
CAGGCTCAGA TAATGGGACC AGGACAAAGC CCAGGACAAA GACTTTCAGT ACCAGTCAAG 
GTGGTACTGC AGCCACAGGC TGGCTCTTCC CAAGGGGCCT CTTCTGGGCT CTCTGTAGTT 
AAAGTTCTGA GTGCCAGTGA AGTGGCAGCT TTGTCATCAC CAGCAAGCTC TGCTCCTCAT 
TCGGGGGGAA AGACAGGAAT GGAGGAAAAC CGCAGATTGG AACACCAGAA GAAGCAAGAG 
AAAGCAAATC GGATTGTAGC AGAGGCCATT GCGAGAGCCC GTGCCCGCGG TGAGCAGAAC 
ATACCTCGAG TCTTAAATGA GGACGAGTTG CCCAGCGTTC GGCCAGAGGA GGAAGGCGAG 
AAGAAACGCA GGAAGAAGAG TGCTGGGGAG AGGCTGAAAG AGGAGAAGCC AAAGAAGAGT 
AAAACATCTG GTGCCTCCAA AACAAAGGGC AAGAGCAAGC TCAACACCAT CACTCCTGTA 
GTGGGTAAGA AGAGAAAACG TAATACCTCA TCTGATAATT CAGATGTGGA AGTCATGCCT 
GCACAGTCAC CTCGAGAAGA TGAAGAAAGC AGCATTCAGA AGAGACGCTC AAACCGCCAA 
GTTAAGCGAA AAAAATATAC AGAGGACCTG GATATAAAGA TCACAGATGA TGAAGAAGAA 
GAAGAGGTGG ATGTAACTGG TCCAATAAAA CCTGAGCCTA TCCTCCCTGA ACCAGTGCAA 
GAACCAGATG GCGAGACTTT GCCTTCCATG CAGTTCTTTG TGGAGAATCC CAGTGAAGAA 
GATGCAGCCA TTGTAGACAA AGTGCTTTCT ATGCGGATTG TGAAGAAGGA GCTCCCTTCT 
GGACAATATA CTGAAGCAGA AGAATTCTTT GTCAAGTACA AGAACTACTC CTATCTGCAT 
TGTGAATGGG CTACTATCTC CCAACTAGAG AAGGATAAGA GGATACATCA AAAATTAAAG 
CGCTTCAAAA CCAAAATGGC TCAGATGAGA CACTTCTTCC ATGAGGATGA AGAGCCCTTT 
AATCCAGACT ACGTAGAGGT GGATAGGATA TTGGATGAGT CTCACAGTAT TGACAAGGAC 
AATGGGGAGC CCGTTATTTA CTACCTGGTG AAATGGTGCT CTCTGCCCTA TGAGGATAGC 
ACATGGGAGC TAAAAGAAGA TGTTGATGAG GGCAAGATTC GAGAATTTAA ACGGATTCAG 
TCAAGGCACC CAGAACTCAA AAGGGTGAAT CGTCCGCAGG CAAGTGCCTG GAAGAAATTG 
GAGCTATCAC ATGAATATAA AAACAGAAAC CAGCTACGGG AATATCAGTT GGAAGGGGTT 
AATTGGCTGC TCTTTAATTG GTATAACAGG CAGAACTGCA TCCTGGCTGA TGAGATGGGA 
TTGGGCAAAA CTATTCAGTC CATTGCCTTC TTGCAGGAAG TATATAATGT GGGCATCCAT 
GGTCCCTTCT TGGTCATTGC CCCACTGTCC ACAATTACTA ACTGGGAGCG AGAATTTAAT 
ACATGGACAG AAATGAACAC TATTGTGTAC CATGGCAGTC TGGCCAGCAG GCAGATGATT 
CAACAGTATG AAATGTACTG CAAAGATTCA CGGGGACGCC TCATCCCAGG CGCATACAAG 
TTTGACGCTC TGATCACCAC TTTTGAGATG ATTTTGTCAG ATTGTCCTGA GCTTCGTGAA 
ATTGAATGGC GTTGTGTTAT CATTGATGAA GCCCATCGAC TGAAAAACCG TAATTGCAAG 
CTGCTTGATA GTCTCAAGCA CATGGACCTG GAACACAAGG TGCTACTCAC AGGAACACCA 
TTGCAAAATA CTGTAGAAGA ACTGTTTAGC TTGCTTCATT TCTTGGAACC GTCACAATTT 
CCCTCAGAAT CAGAGTTTCT CAAGGACTTT GGGGATCTCA AGACAGAGGA ACAGGTTCAA 
AAGCTACAGG CCATTCTTAA GCCAATGATG CTGAGAAGAC TCAAAGAGGA TGTTGAAAAA 
AACTTGGCAC CCAAACAGGA AACAATTATT GAAGTAGAGC TGACTAATAT CCAGAAGAAA 
TACTATCGGG CTATTTTGGA GAAGAATTTC TCCTTCCTTT CCAAAGGGGC AGGTCATACC 
AACATGCCTA ATCTACTTAA CACAATGATG GAGTTGCGCA AGTGCTGCAA CCACCCATAT 
CTCATCAATG GTGCTGAAGA AAAAATCCTA ACAGAATTCC GTGAAGCTTG CCATATTATA 
CCTCATGACT TTCACCTGCA GGCCATGGTT CGTTCAGCCG GCAAACTGGT TCTTATTGAC 
AAGTTGCTTC CAAAGCTTAA AGCTGGTGGC CATAAAGTTC TGATCTTCTC TCAGATGGTG 
CGCTGCCTAG ACATCCTAGA GGATTATTTA ATCCAGAGGA GGTACTTATA TGAACGTATT 
GATGGGCGAG TTAGAGGCAA CCTTCGACAG GCTGCCATTG ACCGCTTCAG CAAGCCTGAC 
TCAGACCGCT TTGTCTTCTT ACTGTGTACC CGGGCTGGTG GACTTGGTAT TAATCTTACA 
GCTGCTGATA CCTGCATCAT CTTTGATTCA GACTGGAATC CACAAAATGA CCTGCAGGCC 
CAGGCACGAT GTCATCGAAT TGGGCAGAGC AAAGCTGTGA AGGTGTACCG CCTCATCACT 
CGTAATTCCT ACGAGAGAGA GATGTTTGAT AAGGCCAGCC TCAAGTTGGG GTTGGATAAG 
GCTGTGCTTC AATCCATGAG TGGTCGGGAT GGCAACATTA CTGGAATCCA ACAGTTCTCT 
AAGAAGGAGA TTGAAGATCT TTTAAGAAAA GGAGCATATG CAGCCATCAT GGAGGAAGAT 
GATGAAGGCT CCAAGTTTTG TGAAGAGGAC ATTGACCAGA TCTTGTTAAG ACGAACTACA 
ACCATCACCA TTGAATCTGA AGGAAAAGGT TCCACCTTTG CTAAGGCAAG CTTTGTTGCT 
TCTGAAAACA GGACAGATAT TTCTTTGGAT GACCCCAACT TTTGGCAAAA GTGGGCCAAA 
AAGGCTGACC TAGACATGGA TCTGCTCAAC AGCAAGAATA ATTTGGTAAT TGACACACCT 
AGAGTACGAA AACAAACGCG CCACTTTAGC ACTCTGAAAG ATGATGACCT GGTGGAATTC 
TCTGATTTGG AAAGTGAGGA TGATGAGCGG CCACGCTCCC GCAGACATGA CCGTCATCAT 
GCCTATGGGC GCACTGACTG CTTTCGGGTG GAAAAGCATC TCCTGGTATA TGGTTGGGGA 
CGATGGCGAG ATATTTTATC TCATGGACGC TTCAAGCGAC GTATGACTGA ACGAGATGTG 
GAGACCATTT GTCGGGCCAT TCTCGTGTAC TGTCTTCTAC ACTACCGTGG GGATGAAAAT 
ATTAAAGGCT TCATCTGGGA CTTGATTAGC CCAGCTGAAA ATGGCAAGAC AAAAGAATTG 
CAGAATCATT CAGGTCTATC TATCCCTGTG CCTCGTGGAC GCAAAGGAAA AAAAGTAAAG 
TCACAAAGCA CTTTTGATAT CCATAAGGCA GATTGGATCC GGAAATATAA CCCTGACACT 
TTGTTCCAAG ATGAAAGTTA TAAGAAGCAC TTGAAACATC AGTGTAACAA GGTACTGTTG 
CGGGTACGAA TGCTATACTA CCTGAGGCAG GAGGTTATTG GAGACCAAGC AGAAAAGGTG 
TTAGGGGGTG CGATTGCCAG TGAGATTGAC ATATGGTTCC CAGTAGTGGA TCAACTGGAG 
GTTCCAACAA CTTGGTGGGA CAGTGAGGCT GACAAGTCGC TGCTCATTGG AGTCTTTAAA 
CATGGCTATG AGAAATATAA TACCATGAGG GCAGACCCAG CCTTATGTTT CCTAGAAAAG 
GCTGGCCGAC CAGATGACAA AGCAATTGCA GCAGAACATC GAGTGTTGGA TAACTTCTCT 
GACATAGTAG AAGGGGTTGA CTTTGATAAA GATTGTGAAG ATCCTGAATA TAAACCACTC 
CAAGGTCCCC CAAAGGACCA AGATGATGAG GGTGATCCCT TGATGATGAT GGATGAGGAG 
ATCTCAGTGA TTGATGGAGA TGAAGCCCAG GTGACCCAAC AGCCAGGCCA TTTATTCTGG 
CCTCCGGGCT CTGCCCTAAC AGCTAGGCTT CGGCGTCTAG TAACAGCGTA TCAGCGCAGC 
TACAAGAGAG AACAAATGAA GATAGAGGCT GCAGAACGTG GGGACCGGCG AAGGCGGCGT 
TGTGAAGCAG CCTTCAAGCT GAAAGAAATT GCACGGCGGG AGAAACAACA ACGATGGACA 
AGGCGTGAAC AAACTGATTT TTATCGAGTG GTGTCTACGT TTGGTGTGGA ATATGACCCT 
GACACCATGC AGTTCCATTG GGATCGCTTC CGCACTTTTG CTCGACTAGA CAAAAAGACA 
GATGAAAGCC TTACCAAGTA CTTCCATGGC TTTGTGGCCA TGTGCCGCCA AGTATGCCGC 
CTTCCCCCAG CAGCTGGAGA TGAACCCCCC GACCCTAACC TGTTCATTGA GCCCATCACT 
GAGGAGAGAG CCTCACGGAC TCTCTACCGT ATAGAATTGC TTCGGCGCTT ACGGGAACAA 
GTTTTATGCC ACCCCCTTTT GGAAGATCGG CTGGCATTGT GTCAGCCTCC TGGTCCTGAA 
TTGCCCAAAT GGTGGGAGCC TGTTCGGCAT GATGGGGAGC TTCTAAGAGG GGCAGCCCGC 
CATGGGGTGA GCCAAACAGA CTGCAACATC ATGCAGGACC CAGACTTCTC TTTTCTGGCT 
GCCCGTATGA ATTATATGCA GAACCATCAA GCAGGAGCAC CAGCTCCATC CTTGTCACGC 
TGCTCTACTC CACTGCTGCA CCAGCAGTAT ACCTCACGCA CTGCCTCACC ACTGCCCCTG 
CGCCCAGATG CTCCTGTTGA AAAGTCACCC GAGGAGACAG CTACCCAGGT CCCCAGTCTG 
GAGAGTCTGA CTTTAAAGCT AGAGCACGAG GTGGTGGCCA GGAGCCGACC AACCCCACAA 
GACTATGAGA TGCGAGTATC CCCCTCTGAT ACTACCCCTC TGGTTTCCCG GAGTGTTCCA 
CCAGTCAAAC TGGAGGATGA GGATGATTCG GACTCTGAGC TGGACTTGAG CAAGCTGTCA 
CCATCTTCTT CTTCTTCCTC ATCCTCATCC AGCTCCAGCT CCAGCACTGA TGAGAGTGAG 
GATGAGAAGG AAGAGAAGCT AACTGACCAG TCCCGCTCAA AGCTCTATGA TGAAGAGAGT 
CTCCTGTCCC TCACTATGTC CCAAGATGGA TTCCCAAATG AAGATGGAGA ACAAATGACC 
CCTGAGCTTC TGCTACTGCA GGAAAGACAA AGAGCCTCTG AGTGGCCCAA GGATCGTGTC 
CTGATAAACC GTATTGACCT CGTCTGCCAG GCTGTACTCT CAGGGAAGTG GCCTTCTAGC 
CGTAGGAGCC AGGAAATGGT AACAGGAGGA ATTTTGGGGC CAGGCAACCA CTTGCTAGAC 
AGTCCCTCAT TGACTCCTGG AGAATATGGT GACTCTCCAG TCCCCACACC ACGAAGTAGT 
AGTGCAGCTT CCATGGCAGA GGAGGAAGCA TCTGCAGTCA GCACAGCGGC AGCCCAGTTC 
ACCAAACTTC GCCGAGGCAT GGATGAAAAG GAGTTTACAG TTCAAATCAA AGATGAGGAA 
GGATTGAAGT TAACATTCCA GAAGCACAAG TTGATGGCGA ATGGAGTAAT GGGAGATGGA 
CATCCACTGT TTCATAAGAA GAAGGGGAAC AGAAAGAAGC TAGTAGAGCT GGAGGTGGAG 
TGCATGGAAG AGCCTAATCA CCTTGATGTG GACCTGGAGA CCCGGATCCC TGTCATCAAT 
AAGGTGGATG GTACTTTGCT GGTGGGTGAG GATGCCCCTC GCCGGGCTGA ACTGGAGATG 
TGGTTACAGG GTCATCCAGA GTTTGCTGTT GATCCCCGAT TTCTAGCGTA TATGGAGGAT 
CGCAGAAAAC AGAAGTGGCA AAGATGTAAA AAAAATAATA AGGCAGAATT GAACTGTTTG 
GGAATGGAAC CAGTACAGAC AGCTAACTCT AGAAATGGGA AAAAGGGTCA TCACACTGAA 
ACGGTGTTCA ACCGGGTTTT GCCAGGGCCT ATTGCACCAG AGAGCAGCAA GAAGCGGGCC 
CGTAGGATGC GACCAGACCT TTCTAAGATG ATGGCCCTCA TGCAGGGTGG AAGCACTGGG 
TCTCTATCTC TGCATAACAC GTTCCAACAC AGCAGTAGTG GCCTACAGTC TGTGTCATCT 
TTGGGTCACA GCAGTGCCAC TTCTGCATCT TTGCCTTTTA TGCCATTTGT GATGGGTGGT 
GCACCATCAT CCCCTCATGT AGACTCCAGC ACCATGCTTC ATCACCACCA CCACCACCCC 
CACCCCCACC ATCACCACCA TCACCATCCA GGCTTGAGAG CCCCTGGCTA CCCCTCTTCA 
CCAGTGACTA CCGCCTCTGG TACTACCTTG CGGTTGCCAC CACTGCAACC TGAGGAGGAT 
GACGATGAGG ATGAAGAAGA TGATGATGAC TTATCTCAGG GCTATGATAG CTCAGAAAGG 
GACTTCTCAC TCATTGATGA TCCTATGATG CCAGCTAACT CAGACTCCAG TGAAGATGCT 
GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065971.2
CDS	148..7056
Translation	MKGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQQPPSTQPVTLSSVQQAQIMGPGQSPGQRLSVPVKVVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASSAPHSGGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSAGERLKEEKPKKSKTSGASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRIVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSIDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELKRVNRPQASAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILTEFREACHIIPHDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHAYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKGFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDIVEGVDFDKDCEDPEYKPLQGPPKDQDDEGDPLMMMDEEISVIDGDEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKIEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDTMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGPELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGAPAPSLSRCSTPLLHQQYTSRTASPLPLRPDAPVEKSPEETATQVPSLESLTLKLEHEVVARSRPTPQDYEMRVSPSDTTPLVSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTDQSRSKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSSRRSQEMVTGGILGPGNHLLDSPSLTPGEYGDSPVPTPRSSSAASMAEEEASAVSTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHKLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDVDLETRIPVINKVDGTLLVGEDAPRRAELEMWLQGHPEFAVDPRFLAYMEDRRKQKWQRCKKNNKAELNCLGMEPVQTANSRNGKKGHHTETVFNRVLPGPIAPESSKKRARRMRPDLSKMMALMQGGSTGSLSLHNTFQHSSSGLQSVSSLGHSSATSASLPFMPFVMGGAPSSPHVDSSTMLHHHHHHPHPHHHHHHHPGLRAPGYPSSPVTTASGTTLRLPPLQPEEDDDEDEEDDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	NM_020920.3
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020920.3

ORF Insert Sequence:

ATGAAGGGTG AATCGAAACG CATCACCCTG GTCCTCCAGC AGCCACAGTC TGGAGGTCCC 
CAAGGACATC GGCATGTTGT GCTAGGGAGT CTACCAGGCA AGATAGTGTT ACAGGGCAAC 
CAGCTGGCAG CCCTGACTCA AGCCAAGAAT GCCCAAGGGC AGCCTGCCAA GGTAGTAACT 
ATCCAGCTGC AGGTGCAGCA GCCACAGCAA AAAATCCAGA TTGTACCACA ACCACCATCA 
TCGCAGCCAC AGCCCCAGCA GCCACCCTCC ACCCAGCCAG TGACTCTGTC CTCTGTACAG 
CAGGCTCAGA TAATGGGACC AGGACAAAGC CCAGGACAAA GACTTTCAGT ACCAGTCAAG 
GTGGTACTGC AGCCACAGGC TGGCTCTTCC CAAGGGGCCT CTTCTGGGCT CTCTGTAGTT 
AAAGTTCTGA GTGCCAGTGA AGTGGCAGCT TTGTCATCAC CAGCAAGCTC TGCTCCTCAT 
TCGGGGGGAA AGACAGGAAT GGAGGAAAAC CGCAGATTGG AACACCAGAA GAAGCAAGAG 
AAAGCAAATC GGATTGTAGC AGAGGCCATT GCGAGAGCCC GTGCCCGCGG TGAGCAGAAC 
ATACCTCGAG TCTTAAATGA GGACGAGTTG CCCAGCGTTC GGCCAGAGGA GGAAGGCGAG 
AAGAAACGCA GGAAGAAGAG TGCTGGGGAG AGGCTGAAAG AGGAGAAGCC AAAGAAGAGT 
AAAACATCTG GTGCCTCCAA AACAAAGGGC AAGAGCAAGC TCAACACCAT CACTCCTGTA 
GTGGGTAAGA AGAGAAAACG TAATACCTCA TCTGATAATT CAGATGTGGA AGTCATGCCT 
GCACAGTCAC CTCGAGAAGA TGAAGAAAGC AGCATTCAGA AGAGACGCTC AAACCGCCAA 
GTTAAGCGAA AAAAATATAC AGAGGACCTG GATATAAAGA TCACAGATGA TGAAGAAGAA 
GAAGAGGTGG ATGTAACTGG TCCAATAAAA CCTGAGCCTA TCCTCCCTGA ACCAGTGCAA 
GAACCAGATG GCGAGACTTT GCCTTCCATG CAGTTCTTTG TGGAGAATCC CAGTGAAGAA 
GATGCAGCCA TTGTAGACAA AGTGCTTTCT ATGCGGATTG TGAAGAAGGA GCTCCCTTCT 
GGACAATATA CTGAAGCAGA AGAATTCTTT GTCAAGTACA AGAACTACTC CTATCTGCAT 
TGTGAATGGG CTACTATCTC CCAACTAGAG AAGGATAAGA GGATACATCA AAAATTAAAG 
CGCTTCAAAA CCAAAATGGC TCAGATGAGA CACTTCTTCC ATGAGGATGA AGAGCCCTTT 
AATCCAGACT ACGTAGAGGT GGATAGGATA TTGGATGAGT CTCACAGTAT TGACAAGGAC 
AATGGGGAGC CCGTTATTTA CTACCTGGTG AAATGGTGCT CTCTGCCCTA TGAGGATAGC 
ACATGGGAGC TAAAAGAAGA TGTTGATGAG GGCAAGATTC GAGAATTTAA ACGGATTCAG 
TCAAGGCACC CAGAACTCAA AAGGGTGAAT CGTCCGCAGG CAAGTGCCTG GAAGAAATTG 
GAGCTATCAC ATGAATATAA AAACAGAAAC CAGCTACGGG AATATCAGTT GGAAGGGGTT 
AATTGGCTGC TCTTTAATTG GTATAACAGG CAGAACTGCA TCCTGGCTGA TGAGATGGGA 
TTGGGCAAAA CTATTCAGTC CATTGCCTTC TTGCAGGAAG TATATAATGT GGGCATCCAT 
GGTCCCTTCT TGGTCATTGC CCCACTGTCC ACAATTACTA ACTGGGAGCG AGAATTTAAT 
ACATGGACAG AAATGAACAC TATTGTGTAC CATGGCAGTC TGGCCAGCAG GCAGATGATT 
CAACAGTATG AAATGTACTG CAAAGATTCA CGGGGACGCC TCATCCCAGG CGCATACAAG 
TTTGACGCTC TGATCACCAC TTTTGAGATG ATTTTGTCAG ATTGTCCTGA GCTTCGTGAA 
ATTGAATGGC GTTGTGTTAT CATTGATGAA GCCCATCGAC TGAAAAACCG TAATTGCAAG 
CTGCTTGATA GTCTCAAGCA CATGGACCTG GAACACAAGG TGCTACTCAC AGGAACACCA 
TTGCAAAATA CTGTAGAAGA ACTGTTTAGC TTGCTTCATT TCTTGGAACC GTCACAATTT 
CCCTCAGAAT CAGAGTTTCT CAAGGACTTT GGGGATCTCA AGACAGAGGA ACAGGTTCAA 
AAGCTACAGG CCATTCTTAA GCCAATGATG CTGAGAAGAC TCAAAGAGGA TGTTGAAAAA 
AACTTGGCAC CCAAACAGGA AACAATTATT GAAGTAGAGC TGACTAATAT CCAGAAGAAA 
TACTATCGGG CTATTTTGGA GAAGAATTTC TCCTTCCTTT CCAAAGGGGC AGGTCATACC 
AACATGCCTA ATCTACTTAA CACAATGATG GAGTTGCGCA AGTGCTGCAA CCACCCATAT 
CTCATCAATG GTGCTGAAGA AAAAATCCTA ACAGAATTCC GTGAAGCTTG CCATATTATA 
CCTCATGACT TTCACCTGCA GGCCATGGTT CGTTCAGCCG GCAAACTGGT TCTTATTGAC 
AAGTTGCTTC CAAAGCTTAA AGCTGGTGGC CATAAAGTTC TGATCTTCTC TCAGATGGTG 
CGCTGCCTAG ACATCCTAGA GGATTATTTA ATCCAGAGGA GGTACTTATA TGAACGTATT 
GATGGGCGAG TTAGAGGCAA CCTTCGACAG GCTGCCATTG ACCGCTTCAG CAAGCCTGAC 
TCAGACCGCT TTGTCTTCTT ACTGTGTACC CGGGCTGGTG GACTTGGTAT TAATCTTACA 
GCTGCTGATA CCTGCATCAT CTTTGATTCA GACTGGAATC CACAAAATGA CCTGCAGGCC 
CAGGCACGAT GTCATCGAAT TGGGCAGAGC AAAGCTGTGA AGGTGTACCG CCTCATCACT 
CGTAATTCCT ACGAGAGAGA GATGTTTGAT AAGGCCAGCC TCAAGTTGGG GTTGGATAAG 
GCTGTGCTTC AATCCATGAG TGGTCGGGAT GGCAACATTA CTGGAATCCA ACAGTTCTCT 
AAGAAGGAGA TTGAAGATCT TTTAAGAAAA GGAGCATATG CAGCCATCAT GGAGGAAGAT 
GATGAAGGCT CCAAGTTTTG TGAAGAGGAC ATTGACCAGA TCTTGTTAAG ACGAACTACA 
ACCATCACCA TTGAATCTGA AGGAAAAGGT TCCACCTTTG CTAAGGCAAG CTTTGTTGCT 
TCTGAAAACA GGACAGATAT TTCTTTGGAT GACCCCAACT TTTGGCAAAA GTGGGCCAAA 
AAGGCTGACC TAGACATGGA TCTGCTCAAC AGCAAGAATA ATTTGGTAAT TGACACACCT 
AGAGTACGAA AACAAACGCG CCACTTTAGC ACTCTGAAAG ATGATGACCT GGTGGAATTC 
TCTGATTTGG AAAGTGAGGA TGATGAGCGG CCACGCTCCC GCAGACATGA CCGTCATCAT 
GCCTATGGGC GCACTGACTG CTTTCGGGTG GAAAAGCATC TCCTGGTATA TGGTTGGGGA 
CGATGGCGAG ATATTTTATC TCATGGACGC TTCAAGCGAC GTATGACTGA ACGAGATGTG 
GAGACCATTT GTCGGGCCAT TCTCGTGTAC TGTCTTCTAC ACTACCGTGG GGATGAAAAT 
ATTAAAGGCT TCATCTGGGA CTTGATTAGC CCAGCTGAAA ATGGCAAGAC AAAAGAATTG 
CAGAATCATT CAGGTCTATC TATCCCTGTG CCTCGTGGAC GCAAAGGAAA AAAAGTAAAG 
TCACAAAGCA CTTTTGATAT CCATAAGGCA GATTGGATCC GGAAATATAA CCCTGACACT 
TTGTTCCAAG ATGAAAGTTA TAAGAAGCAC TTGAAACATC AGTGTAACAA GGTACTGTTG 
CGGGTACGAA TGCTATACTA CCTGAGGCAG GAGGTTATTG GAGACCAAGC AGAAAAGGTG 
TTAGGGGGTG CGATTGCCAG TGAGATTGAC ATATGGTTCC CAGTAGTGGA TCAACTGGAG 
GTTCCAACAA CTTGGTGGGA CAGTGAGGCT GACAAGTCGC TGCTCATTGG AGTCTTTAAA 
CATGGCTATG AGAAATATAA TACCATGAGG GCAGACCCAG CCTTATGTTT CCTAGAAAAG 
GCTGGCCGAC CAGATGACAA AGCAATTGCA GCAGAACATC GAGTGTTGGA TAACTTCTCT 
GACATAGTAG AAGGGGTTGA CTTTGATAAA GATTGTGAAG ATCCTGAATA TAAACCACTC 
CAAGGTCCCC CAAAGGACCA AGATGATGAG GGTGATCCCT TGATGATGAT GGATGAGGAG 
ATCTCAGTGA TTGATGGAGA TGAAGCCCAG GTGACCCAAC AGCCAGGCCA TTTATTCTGG 
CCTCCGGGCT CTGCCCTAAC AGCTAGGCTT CGGCGTCTAG TAACAGCGTA TCAGCGCAGC 
TACAAGAGAG AACAAATGAA GATAGAGGCT GCAGAACGTG GGGACCGGCG AAGGCGGCGT 
TGTGAAGCAG CCTTCAAGCT GAAAGAAATT GCACGGCGGG AGAAACAACA ACGATGGACA 
AGGCGTGAAC AAACTGATTT TTATCGAGTG GTGTCTACGT TTGGTGTGGA ATATGACCCT 
GACACCATGC AGTTCCATTG GGATCGCTTC CGCACTTTTG CTCGACTAGA CAAAAAGACA 
GATGAAAGCC TTACCAAGTA CTTCCATGGC TTTGTGGCCA TGTGCCGCCA AGTATGCCGC 
CTTCCCCCAG CAGCTGGAGA TGAACCCCCC GACCCTAACC TGTTCATTGA GCCCATCACT 
GAGGAGAGAG CCTCACGGAC TCTCTACCGT ATAGAATTGC TTCGGCGCTT ACGGGAACAA 
GTTTTATGCC ACCCCCTTTT GGAAGATCGG CTGGCATTGT GTCAGCCTCC TGGTCCTGAA 
TTGCCCAAAT GGTGGGAGCC TGTTCGGCAT GATGGGGAGC TTCTAAGAGG GGCAGCCCGC 
CATGGGGTGA GCCAAACAGA CTGCAACATC ATGCAGGACC CAGACTTCTC TTTTCTGGCT 
GCCCGTATGA ATTATATGCA GAACCATCAA GCAGGAGCAC CAGCTCCATC CTTGTCACGC 
TGCTCTACTC CACTGCTGCA CCAGCAGTAT ACCTCACGCA CTGCCTCACC ACTGCCCCTG 
CGCCCAGATG CTCCTGTTGA AAAGTCACCC GAGGAGACAG CTACCCAGGT CCCCAGTCTG 
GAGAGTCTGA CTTTAAAGCT AGAGCACGAG GTGGTGGCCA GGAGCCGACC AACCCCACAA 
GACTATGAGA TGCGAGTATC CCCCTCTGAT ACTACCCCTC TGGTTTCCCG GAGTGTTCCA 
CCAGTCAAAC TGGAGGATGA GGATGATTCG GACTCTGAGC TGGACTTGAG CAAGCTGTCA 
CCATCTTCTT CTTCTTCCTC ATCCTCATCC AGCTCCAGCT CCAGCACTGA TGAGAGTGAG 
GATGAGAAGG AAGAGAAGCT AACTGACCAG TCCCGCTCAA AGCTCTATGA TGAAGAGAGT 
CTCCTGTCCC TCACTATGTC CCAAGATGGA TTCCCAAATG AAGATGGAGA ACAAATGACC 
CCTGAGCTTC TGCTACTGCA GGAAAGACAA AGAGCCTCTG AGTGGCCCAA GGATCGTGTC 
CTGATAAACC GTATTGACCT CGTCTGCCAG GCTGTACTCT CAGGGAAGTG GCCTTCTAGC 
CGTAGGAGCC AGGAAATGGT AACAGGAGGA ATTTTGGGGC CAGGCAACCA CTTGCTAGAC 
AGTCCCTCAT TGACTCCTGG AGAATATGGT GACTCTCCAG TCCCCACACC ACGAAGTAGT 
AGTGCAGCTT CCATGGCAGA GGAGGAAGCA TCTGCAGTCA GCACAGCGGC AGCCCAGTTC 
ACCAAACTTC GCCGAGGCAT GGATGAAAAG GAGTTTACAG TTCAAATCAA AGATGAGGAA 
GGATTGAAGT TAACATTCCA GAAGCACAAG TTGATGGCGA ATGGAGTAAT GGGAGATGGA 
CATCCACTGT TTCATAAGAA GAAGGGGAAC AGAAAGAAGC TAGTAGAGCT GGAGGTGGAG 
TGCATGGAAG AGCCTAATCA CCTTGATGTG GACCTGGAGA CCCGGATCCC TGTCATCAAT 
AAGGTGGATG GTACTTTGCT GGTGGGTGAG GATGCCCCTC GCCGGGCTGA ACTGGAGATG 
TGGTTACAGG GTCATCCAGA GTTTGCTGTT GATCCCCGAT TTCTAGCGTA TATGGAGGAT 
CGCAGAAAAC AGAAGTGGCA AAGATGTAAA AAAAATAATA AGGCAGAATT GAACTGTTTG 
GGAATGGAAC CAGTACAGAC AGCTAACTCT AGAAATGGGA AAAAGGGTCA TCACACTGAA 
ACGGTGTTCA ACCGGGTTTT GCCAGGGCCT ATTGCACCAG AGAGCAGCAA GAAGCGGGCC 
CGTAGGATGC GACCAGACCT TTCTAAGATG ATGGCCCTCA TGCAGGGTGG AAGCACTGGG 
TCTCTATCTC TGCATAACAC GTTCCAACAC AGCAGTAGTG GCCTACAGTC TGTGTCATCT 
TTGGGTCACA GCAGTGCCAC TTCTGCATCT TTGCCTTTTA TGCCATTTGT GATGGGTGGT 
GCACCATCAT CCCCTCATGT AGACTCCAGC ACCATGCTTC ATCACCACCA CCACCACCCC 
CACCCCCACC ATCACCACCA TCACCATCCA GGCTTGAGAG CCCCTGGCTA CCCCTCTTCA 
CCAGTGACTA CCGCCTCTGG TACTACCTTG CGGTTGCCAC CACTGCAACC TGAGGAGGAT 
GACGATGAGG ATGAAGAAGA TGATGATGAC TTATCTCAGG GCTATGATAG CTCAGAAAGG 
GACTTCTCAC TCATTGATGA TCCTATGATG CCAGCTAACT CAGACTCCAG TGAAGATGCT 
GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CHD8 ( NM_020920.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_065971.2 Homo sapiens chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 2, mRNA.

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CloneID	OHu17867
Clone ID Related Accession (Same CDS sequence)	NM_020920.3 , NM_020920.4
Accession Version	NM_020920.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6909bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-29
Organism	Homo sapiens(human)
Product	chromodomain-helicase-DNA-binding protein 8 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN290520.1, AK122910.1, AB046784.3, AL834524.1 and BC036920.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_020920.3. Transcript Variant: This variant (2) represents use of an alternate promoter and 5' UTR and has a distinct 5' coding region, compared to variant 1. The resulting isoform (2) has a shorter and distinct N-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: CR749315.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAAGGGTG AATCGAAACG CATCACCCTG GTCCTCCAGC AGCCACAGTC TGGAGGTCCC 
CAAGGACATC GGCATGTTGT GCTAGGGAGT CTACCAGGCA AGATAGTGTT ACAGGGCAAC 
CAGCTGGCAG CCCTGACTCA AGCCAAGAAT GCCCAAGGGC AGCCTGCCAA GGTAGTAACT 
ATCCAGCTGC AGGTGCAGCA GCCACAGCAA AAAATCCAGA TTGTACCACA ACCACCATCA 
TCGCAGCCAC AGCCCCAGCA GCCACCCTCC ACCCAGCCAG TGACTCTGTC CTCTGTACAG 
CAGGCTCAGA TAATGGGACC AGGACAAAGC CCAGGACAAA GACTTTCAGT ACCAGTCAAG 
GTGGTACTGC AGCCACAGGC TGGCTCTTCC CAAGGGGCCT CTTCTGGGCT CTCTGTAGTT 
AAAGTTCTGA GTGCCAGTGA AGTGGCAGCT TTGTCATCAC CAGCAAGCTC TGCTCCTCAT 
TCGGGGGGAA AGACAGGAAT GGAGGAAAAC CGCAGATTGG AACACCAGAA GAAGCAAGAG 
AAAGCAAATC GGATTGTAGC AGAGGCCATT GCGAGAGCCC GTGCCCGCGG TGAGCAGAAC 
ATACCTCGAG TCTTAAATGA GGACGAGTTG CCCAGCGTTC GGCCAGAGGA GGAAGGCGAG 
AAGAAACGCA GGAAGAAGAG TGCTGGGGAG AGGCTGAAAG AGGAGAAGCC AAAGAAGAGT 
AAAACATCTG GTGCCTCCAA AACAAAGGGC AAGAGCAAGC TCAACACCAT CACTCCTGTA 
GTGGGTAAGA AGAGAAAACG TAATACCTCA TCTGATAATT CAGATGTGGA AGTCATGCCT 
GCACAGTCAC CTCGAGAAGA TGAAGAAAGC AGCATTCAGA AGAGACGCTC AAACCGCCAA 
GTTAAGCGAA AAAAATATAC AGAGGACCTG GATATAAAGA TCACAGATGA TGAAGAAGAA 
GAAGAGGTGG ATGTAACTGG TCCAATAAAA CCTGAGCCTA TCCTCCCTGA ACCAGTGCAA 
GAACCAGATG GCGAGACTTT GCCTTCCATG CAGTTCTTTG TGGAGAATCC CAGTGAAGAA 
GATGCAGCCA TTGTAGACAA AGTGCTTTCT ATGCGGATTG TGAAGAAGGA GCTCCCTTCT 
GGACAATATA CTGAAGCAGA AGAATTCTTT GTCAAGTACA AGAACTACTC CTATCTGCAT 
TGTGAATGGG CTACTATCTC CCAACTAGAG AAGGATAAGA GGATACATCA AAAATTAAAG 
CGCTTCAAAA CCAAAATGGC TCAGATGAGA CACTTCTTCC ATGAGGATGA AGAGCCCTTT 
AATCCAGACT ACGTAGAGGT GGATAGGATA TTGGATGAGT CTCACAGTAT TGACAAGGAC 
AATGGGGAGC CCGTTATTTA CTACCTGGTG AAATGGTGCT CTCTGCCCTA TGAGGATAGC 
ACATGGGAGC TAAAAGAAGA TGTTGATGAG GGCAAGATTC GAGAATTTAA ACGGATTCAG 
TCAAGGCACC CAGAACTCAA AAGGGTGAAT CGTCCGCAGG CAAGTGCCTG GAAGAAATTG 
GAGCTATCAC ATGAATATAA AAACAGAAAC CAGCTACGGG AATATCAGTT GGAAGGGGTT 
AATTGGCTGC TCTTTAATTG GTATAACAGG CAGAACTGCA TCCTGGCTGA TGAGATGGGA 
TTGGGCAAAA CTATTCAGTC CATTGCCTTC TTGCAGGAAG TATATAATGT GGGCATCCAT 
GGTCCCTTCT TGGTCATTGC CCCACTGTCC ACAATTACTA ACTGGGAGCG AGAATTTAAT 
ACATGGACAG AAATGAACAC TATTGTGTAC CATGGCAGTC TGGCCAGCAG GCAGATGATT 
CAACAGTATG AAATGTACTG CAAAGATTCA CGGGGACGCC TCATCCCAGG CGCATACAAG 
TTTGACGCTC TGATCACCAC TTTTGAGATG ATTTTGTCAG ATTGTCCTGA GCTTCGTGAA 
ATTGAATGGC GTTGTGTTAT CATTGATGAA GCCCATCGAC TGAAAAACCG TAATTGCAAG 
CTGCTTGATA GTCTCAAGCA CATGGACCTG GAACACAAGG TGCTACTCAC AGGAACACCA 
TTGCAAAATA CTGTAGAAGA ACTGTTTAGC TTGCTTCATT TCTTGGAACC GTCACAATTT 
CCCTCAGAAT CAGAGTTTCT CAAGGACTTT GGGGATCTCA AGACAGAGGA ACAGGTTCAA 
AAGCTACAGG CCATTCTTAA GCCAATGATG CTGAGAAGAC TCAAAGAGGA TGTTGAAAAA 
AACTTGGCAC CCAAACAGGA AACAATTATT GAAGTAGAGC TGACTAATAT CCAGAAGAAA 
TACTATCGGG CTATTTTGGA GAAGAATTTC TCCTTCCTTT CCAAAGGGGC AGGTCATACC 
AACATGCCTA ATCTACTTAA CACAATGATG GAGTTGCGCA AGTGCTGCAA CCACCCATAT 
CTCATCAATG GTGCTGAAGA AAAAATCCTA ACAGAATTCC GTGAAGCTTG CCATATTATA 
CCTCATGACT TTCACCTGCA GGCCATGGTT CGTTCAGCCG GCAAACTGGT TCTTATTGAC 
AAGTTGCTTC CAAAGCTTAA AGCTGGTGGC CATAAAGTTC TGATCTTCTC TCAGATGGTG 
CGCTGCCTAG ACATCCTAGA GGATTATTTA ATCCAGAGGA GGTACTTATA TGAACGTATT 
GATGGGCGAG TTAGAGGCAA CCTTCGACAG GCTGCCATTG ACCGCTTCAG CAAGCCTGAC 
TCAGACCGCT TTGTCTTCTT ACTGTGTACC CGGGCTGGTG GACTTGGTAT TAATCTTACA 
GCTGCTGATA CCTGCATCAT CTTTGATTCA GACTGGAATC CACAAAATGA CCTGCAGGCC 
CAGGCACGAT GTCATCGAAT TGGGCAGAGC AAAGCTGTGA AGGTGTACCG CCTCATCACT 
CGTAATTCCT ACGAGAGAGA GATGTTTGAT AAGGCCAGCC TCAAGTTGGG GTTGGATAAG 
GCTGTGCTTC AATCCATGAG TGGTCGGGAT GGCAACATTA CTGGAATCCA ACAGTTCTCT 
AAGAAGGAGA TTGAAGATCT TTTAAGAAAA GGAGCATATG CAGCCATCAT GGAGGAAGAT 
GATGAAGGCT CCAAGTTTTG TGAAGAGGAC ATTGACCAGA TCTTGTTAAG ACGAACTACA 
ACCATCACCA TTGAATCTGA AGGAAAAGGT TCCACCTTTG CTAAGGCAAG CTTTGTTGCT 
TCTGAAAACA GGACAGATAT TTCTTTGGAT GACCCCAACT TTTGGCAAAA GTGGGCCAAA 
AAGGCTGACC TAGACATGGA TCTGCTCAAC AGCAAGAATA ATTTGGTAAT TGACACACCT 
AGAGTACGAA AACAAACGCG CCACTTTAGC ACTCTGAAAG ATGATGACCT GGTGGAATTC 
TCTGATTTGG AAAGTGAGGA TGATGAGCGG CCACGCTCCC GCAGACATGA CCGTCATCAT 
GCCTATGGGC GCACTGACTG CTTTCGGGTG GAAAAGCATC TCCTGGTATA TGGTTGGGGA 
CGATGGCGAG ATATTTTATC TCATGGACGC TTCAAGCGAC GTATGACTGA ACGAGATGTG 
GAGACCATTT GTCGGGCCAT TCTCGTGTAC TGTCTTCTAC ACTACCGTGG GGATGAAAAT 
ATTAAAGGCT TCATCTGGGA CTTGATTAGC CCAGCTGAAA ATGGCAAGAC AAAAGAATTG 
CAGAATCATT CAGGTCTATC TATCCCTGTG CCTCGTGGAC GCAAAGGAAA AAAAGTAAAG 
TCACAAAGCA CTTTTGATAT CCATAAGGCA GATTGGATCC GGAAATATAA CCCTGACACT 
TTGTTCCAAG ATGAAAGTTA TAAGAAGCAC TTGAAACATC AGTGTAACAA GGTACTGTTG 
CGGGTACGAA TGCTATACTA CCTGAGGCAG GAGGTTATTG GAGACCAAGC AGAAAAGGTG 
TTAGGGGGTG CGATTGCCAG TGAGATTGAC ATATGGTTCC CAGTAGTGGA TCAACTGGAG 
GTTCCAACAA CTTGGTGGGA CAGTGAGGCT GACAAGTCGC TGCTCATTGG AGTCTTTAAA 
CATGGCTATG AGAAATATAA TACCATGAGG GCAGACCCAG CCTTATGTTT CCTAGAAAAG 
GCTGGCCGAC CAGATGACAA AGCAATTGCA GCAGAACATC GAGTGTTGGA TAACTTCTCT 
GACATAGTAG AAGGGGTTGA CTTTGATAAA GATTGTGAAG ATCCTGAATA TAAACCACTC 
CAAGGTCCCC CAAAGGACCA AGATGATGAG GGTGATCCCT TGATGATGAT GGATGAGGAG 
ATCTCAGTGA TTGATGGAGA TGAAGCCCAG GTGACCCAAC AGCCAGGCCA TTTATTCTGG 
CCTCCGGGCT CTGCCCTAAC AGCTAGGCTT CGGCGTCTAG TAACAGCGTA TCAGCGCAGC 
TACAAGAGAG AACAAATGAA GATAGAGGCT GCAGAACGTG GGGACCGGCG AAGGCGGCGT 
TGTGAAGCAG CCTTCAAGCT GAAAGAAATT GCACGGCGGG AGAAACAACA ACGATGGACA 
AGGCGTGAAC AAACTGATTT TTATCGAGTG GTGTCTACGT TTGGTGTGGA ATATGACCCT 
GACACCATGC AGTTCCATTG GGATCGCTTC CGCACTTTTG CTCGACTAGA CAAAAAGACA 
GATGAAAGCC TTACCAAGTA CTTCCATGGC TTTGTGGCCA TGTGCCGCCA AGTATGCCGC 
CTTCCCCCAG CAGCTGGAGA TGAACCCCCC GACCCTAACC TGTTCATTGA GCCCATCACT 
GAGGAGAGAG CCTCACGGAC TCTCTACCGT ATAGAATTGC TTCGGCGCTT ACGGGAACAA 
GTTTTATGCC ACCCCCTTTT GGAAGATCGG CTGGCATTGT GTCAGCCTCC TGGTCCTGAA 
TTGCCCAAAT GGTGGGAGCC TGTTCGGCAT GATGGGGAGC TTCTAAGAGG GGCAGCCCGC 
CATGGGGTGA GCCAAACAGA CTGCAACATC ATGCAGGACC CAGACTTCTC TTTTCTGGCT 
GCCCGTATGA ATTATATGCA GAACCATCAA GCAGGAGCAC CAGCTCCATC CTTGTCACGC 
TGCTCTACTC CACTGCTGCA CCAGCAGTAT ACCTCACGCA CTGCCTCACC ACTGCCCCTG 
CGCCCAGATG CTCCTGTTGA AAAGTCACCC GAGGAGACAG CTACCCAGGT CCCCAGTCTG 
GAGAGTCTGA CTTTAAAGCT AGAGCACGAG GTGGTGGCCA GGAGCCGACC AACCCCACAA 
GACTATGAGA TGCGAGTATC CCCCTCTGAT ACTACCCCTC TGGTTTCCCG GAGTGTTCCA 
CCAGTCAAAC TGGAGGATGA GGATGATTCG GACTCTGAGC TGGACTTGAG CAAGCTGTCA 
CCATCTTCTT CTTCTTCCTC ATCCTCATCC AGCTCCAGCT CCAGCACTGA TGAGAGTGAG 
GATGAGAAGG AAGAGAAGCT AACTGACCAG TCCCGCTCAA AGCTCTATGA TGAAGAGAGT 
CTCCTGTCCC TCACTATGTC CCAAGATGGA TTCCCAAATG AAGATGGAGA ACAAATGACC 
CCTGAGCTTC TGCTACTGCA GGAAAGACAA AGAGCCTCTG AGTGGCCCAA GGATCGTGTC 
CTGATAAACC GTATTGACCT CGTCTGCCAG GCTGTACTCT CAGGGAAGTG GCCTTCTAGC 
CGTAGGAGCC AGGAAATGGT AACAGGAGGA ATTTTGGGGC CAGGCAACCA CTTGCTAGAC 
AGTCCCTCAT TGACTCCTGG AGAATATGGT GACTCTCCAG TCCCCACACC ACGAAGTAGT 
AGTGCAGCTT CCATGGCAGA GGAGGAAGCA TCTGCAGTCA GCACAGCGGC AGCCCAGTTC 
ACCAAACTTC GCCGAGGCAT GGATGAAAAG GAGTTTACAG TTCAAATCAA AGATGAGGAA 
GGATTGAAGT TAACATTCCA GAAGCACAAG TTGATGGCGA ATGGAGTAAT GGGAGATGGA 
CATCCACTGT TTCATAAGAA GAAGGGGAAC AGAAAGAAGC TAGTAGAGCT GGAGGTGGAG 
TGCATGGAAG AGCCTAATCA CCTTGATGTG GACCTGGAGA CCCGGATCCC TGTCATCAAT 
AAGGTGGATG GTACTTTGCT GGTGGGTGAG GATGCCCCTC GCCGGGCTGA ACTGGAGATG 
TGGTTACAGG GTCATCCAGA GTTTGCTGTT GATCCCCGAT TTCTAGCGTA TATGGAGGAT 
CGCAGAAAAC AGAAGTGGCA AAGATGTAAA AAAAATAATA AGGCAGAATT GAACTGTTTG 
GGAATGGAAC CAGTACAGAC AGCTAACTCT AGAAATGGGA AAAAGGGTCA TCACACTGAA 
ACGGTGTTCA ACCGGGTTTT GCCAGGGCCT ATTGCACCAG AGAGCAGCAA GAAGCGGGCC 
CGTAGGATGC GACCAGACCT TTCTAAGATG ATGGCCCTCA TGCAGGGTGG AAGCACTGGG 
TCTCTATCTC TGCATAACAC GTTCCAACAC AGCAGTAGTG GCCTACAGTC TGTGTCATCT 
TTGGGTCACA GCAGTGCCAC TTCTGCATCT TTGCCTTTTA TGCCATTTGT GATGGGTGGT 
GCACCATCAT CCCCTCATGT AGACTCCAGC ACCATGCTTC ATCACCACCA CCACCACCCC 
CACCCCCACC ATCACCACCA TCACCATCCA GGCTTGAGAG CCCCTGGCTA CCCCTCTTCA 
CCAGTGACTA CCGCCTCTGG TACTACCTTG CGGTTGCCAC CACTGCAACC TGAGGAGGAT 
GACGATGAGG ATGAAGAAGA TGATGATGAC TTATCTCAGG GCTATGATAG CTCAGAAAGG 
GACTTCTCAC TCATTGATGA TCCTATGATG CCAGCTAACT CAGACTCCAG TGAAGATGCT 
GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065971.2
CDS	125..7033
Translation	MKGESKRITLVLQQPQSGGPQGHRHVVLGSLPGKIVLQGNQLAALTQAKNAQGQPAKVVTIQLQVQQPQQKIQIVPQPPSSQPQPQQPPSTQPVTLSSVQQAQIMGPGQSPGQRLSVPVKVVLQPQAGSSQGASSGLSVVKVLSASEVAALSSPASSAPHSGGKTGMEENRRLEHQKKQEKANRIVAEAIARARARGEQNIPRVLNEDELPSVRPEEEGEKKRRKKSAGERLKEEKPKKSKTSGASKTKGKSKLNTITPVVGKKRKRNTSSDNSDVEVMPAQSPREDEESSIQKRRSNRQVKRKKYTEDLDIKITDDEEEEEVDVTGPIKPEPILPEPVQEPDGETLPSMQFFVENPSEEDAAIVDKVLSMRIVKKELPSGQYTEAEEFFVKYKNYSYLHCEWATISQLEKDKRIHQKLKRFKTKMAQMRHFFHEDEEPFNPDYVEVDRILDESHSIDKDNGEPVIYYLVKWCSLPYEDSTWELKEDVDEGKIREFKRIQSRHPELKRVNRPQASAWKKLELSHEYKNRNQLREYQLEGVNWLLFNWYNRQNCILADEMGLGKTIQSIAFLQEVYNVGIHGPFLVIAPLSTITNWEREFNTWTEMNTIVYHGSLASRQMIQQYEMYCKDSRGRLIPGAYKFDALITTFEMILSDCPELREIEWRCVIIDEAHRLKNRNCKLLDSLKHMDLEHKVLLTGTPLQNTVEELFSLLHFLEPSQFPSESEFLKDFGDLKTEEQVQKLQAILKPMMLRRLKEDVEKNLAPKQETIIEVELTNIQKKYYRAILEKNFSFLSKGAGHTNMPNLLNTMMELRKCCNHPYLINGAEEKILTEFREACHIIPHDFHLQAMVRSAGKLVLIDKLLPKLKAGGHKVLIFSQMVRCLDILEDYLIQRRYLYERIDGRVRGNLRQAAIDRFSKPDSDRFVFLLCTRAGGLGINLTAADTCIIFDSDWNPQNDLQAQARCHRIGQSKAVKVYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQFSKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASFVASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDLVEFSDLESEDDERPRSRRHDRHHAYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMTERDVETICRAILVYCLLHYRGDENIKGFIWDLISPAENGKTKELQNHSGLSIPVPRGRKGKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIGDQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADPALCFLEKAGRPDDKAIAAEHRVLDNFSDIVEGVDFDKDCEDPEYKPLQGPPKDQDDEGDPLMMMDEEISVIDGDEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKIEAAERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDTMQFHWDRFRTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLYRIELLRRLREQVLCHPLLEDRLALCQPPGPELPKWWEPVRHDGELLRGAARHGVSQTDCNIMQDPDFSFLAARMNYMQNHQAGAPAPSLSRCSTPLLHQQYTSRTASPLPLRPDAPVEKSPEETATQVPSLESLTLKLEHEVVARSRPTPQDYEMRVSPSDTTPLVSRSVPPVKLEDEDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTDQSRSKLYDEESLLSLTMSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSSRRSQEMVTGGILGPGNHLLDSPSLTPGEYGDSPVPTPRSSSAASMAEEEASAVSTAAAQFTKLRRGMDEKEFTVQIKDEEGLKLTFQKHKLMANGVMGDGHPLFHKKKGNRKKLVELEVECMEEPNHLDVDLETRIPVINKVDGTLLVGEDAPRRAELEMWLQGHPEFAVDPRFLAYMEDRRKQKWQRCKKNNKAELNCLGMEPVQTANSRNGKKGHHTETVFNRVLPGPIAPESSKKRARRMRPDLSKMMALMQGGSTGSLSLHNTFQHSSSGLQSVSSLGHSSATSASLPFMPFVMGGAPSSPHVDSSTMLHHHHHHPHPHHHHHHHPGLRAPGYPSSPVTTASGTTLRLPPLQPEEDDDEDEEDDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD

Target ORF information:

RefSeq Version	NM_020920.4
Organism	Homo sapiens(human)
Definition	Homo sapiens chromodomain helicase DNA binding protein 8 (CHD8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020920.4

ORF Insert Sequence:

ATGAAGGGTG AATCGAAACG CATCACCCTG GTCCTCCAGC AGCCACAGTC TGGAGGTCCC 
CAAGGACATC GGCATGTTGT GCTAGGGAGT CTACCAGGCA AGATAGTGTT ACAGGGCAAC 
CAGCTGGCAG CCCTGACTCA AGCCAAGAAT GCCCAAGGGC AGCCTGCCAA GGTAGTAACT 
ATCCAGCTGC AGGTGCAGCA GCCACAGCAA AAAATCCAGA TTGTACCACA ACCACCATCA 
TCGCAGCCAC AGCCCCAGCA GCCACCCTCC ACCCAGCCAG TGACTCTGTC CTCTGTACAG 
CAGGCTCAGA TAATGGGACC AGGACAAAGC CCAGGACAAA GACTTTCAGT ACCAGTCAAG 
GTGGTACTGC AGCCACAGGC TGGCTCTTCC CAAGGGGCCT CTTCTGGGCT CTCTGTAGTT 
AAAGTTCTGA GTGCCAGTGA AGTGGCAGCT TTGTCATCAC CAGCAAGCTC TGCTCCTCAT 
TCGGGGGGAA AGACAGGAAT GGAGGAAAAC CGCAGATTGG AACACCAGAA GAAGCAAGAG 
AAAGCAAATC GGATTGTAGC AGAGGCCATT GCGAGAGCCC GTGCCCGCGG TGAGCAGAAC 
ATACCTCGAG TCTTAAATGA GGACGAGTTG CCCAGCGTTC GGCCAGAGGA GGAAGGCGAG 
AAGAAACGCA GGAAGAAGAG TGCTGGGGAG AGGCTGAAAG AGGAGAAGCC AAAGAAGAGT 
AAAACATCTG GTGCCTCCAA AACAAAGGGC AAGAGCAAGC TCAACACCAT CACTCCTGTA 
GTGGGTAAGA AGAGAAAACG TAATACCTCA TCTGATAATT CAGATGTGGA AGTCATGCCT 
GCACAGTCAC CTCGAGAAGA TGAAGAAAGC AGCATTCAGA AGAGACGCTC AAACCGCCAA 
GTTAAGCGAA AAAAATATAC AGAGGACCTG GATATAAAGA TCACAGATGA TGAAGAAGAA 
GAAGAGGTGG ATGTAACTGG TCCAATAAAA CCTGAGCCTA TCCTCCCTGA ACCAGTGCAA 
GAACCAGATG GCGAGACTTT GCCTTCCATG CAGTTCTTTG TGGAGAATCC CAGTGAAGAA 
GATGCAGCCA TTGTAGACAA AGTGCTTTCT ATGCGGATTG TGAAGAAGGA GCTCCCTTCT 
GGACAATATA CTGAAGCAGA AGAATTCTTT GTCAAGTACA AGAACTACTC CTATCTGCAT 
TGTGAATGGG CTACTATCTC CCAACTAGAG AAGGATAAGA GGATACATCA AAAATTAAAG 
CGCTTCAAAA CCAAAATGGC TCAGATGAGA CACTTCTTCC ATGAGGATGA AGAGCCCTTT 
AATCCAGACT ACGTAGAGGT GGATAGGATA TTGGATGAGT CTCACAGTAT TGACAAGGAC 
AATGGGGAGC CCGTTATTTA CTACCTGGTG AAATGGTGCT CTCTGCCCTA TGAGGATAGC 
ACATGGGAGC TAAAAGAAGA TGTTGATGAG GGCAAGATTC GAGAATTTAA ACGGATTCAG 
TCAAGGCACC CAGAACTCAA AAGGGTGAAT CGTCCGCAGG CAAGTGCCTG GAAGAAATTG 
GAGCTATCAC ATGAATATAA AAACAGAAAC CAGCTACGGG AATATCAGTT GGAAGGGGTT 
AATTGGCTGC TCTTTAATTG GTATAACAGG CAGAACTGCA TCCTGGCTGA TGAGATGGGA 
TTGGGCAAAA CTATTCAGTC CATTGCCTTC TTGCAGGAAG TATATAATGT GGGCATCCAT 
GGTCCCTTCT TGGTCATTGC CCCACTGTCC ACAATTACTA ACTGGGAGCG AGAATTTAAT 
ACATGGACAG AAATGAACAC TATTGTGTAC CATGGCAGTC TGGCCAGCAG GCAGATGATT 
CAACAGTATG AAATGTACTG CAAAGATTCA CGGGGACGCC TCATCCCAGG CGCATACAAG 
TTTGACGCTC TGATCACCAC TTTTGAGATG ATTTTGTCAG ATTGTCCTGA GCTTCGTGAA 
ATTGAATGGC GTTGTGTTAT CATTGATGAA GCCCATCGAC TGAAAAACCG TAATTGCAAG 
CTGCTTGATA GTCTCAAGCA CATGGACCTG GAACACAAGG TGCTACTCAC AGGAACACCA 
TTGCAAAATA CTGTAGAAGA ACTGTTTAGC TTGCTTCATT TCTTGGAACC GTCACAATTT 
CCCTCAGAAT CAGAGTTTCT CAAGGACTTT GGGGATCTCA AGACAGAGGA ACAGGTTCAA 
AAGCTACAGG CCATTCTTAA GCCAATGATG CTGAGAAGAC TCAAAGAGGA TGTTGAAAAA 
AACTTGGCAC CCAAACAGGA AACAATTATT GAAGTAGAGC TGACTAATAT CCAGAAGAAA 
TACTATCGGG CTATTTTGGA GAAGAATTTC TCCTTCCTTT CCAAAGGGGC AGGTCATACC 
AACATGCCTA ATCTACTTAA CACAATGATG GAGTTGCGCA AGTGCTGCAA CCACCCATAT 
CTCATCAATG GTGCTGAAGA AAAAATCCTA ACAGAATTCC GTGAAGCTTG CCATATTATA 
CCTCATGACT TTCACCTGCA GGCCATGGTT CGTTCAGCCG GCAAACTGGT TCTTATTGAC 
AAGTTGCTTC CAAAGCTTAA AGCTGGTGGC CATAAAGTTC TGATCTTCTC TCAGATGGTG 
CGCTGCCTAG ACATCCTAGA GGATTATTTA ATCCAGAGGA GGTACTTATA TGAACGTATT 
GATGGGCGAG TTAGAGGCAA CCTTCGACAG GCTGCCATTG ACCGCTTCAG CAAGCCTGAC 
TCAGACCGCT TTGTCTTCTT ACTGTGTACC CGGGCTGGTG GACTTGGTAT TAATCTTACA 
GCTGCTGATA CCTGCATCAT CTTTGATTCA GACTGGAATC CACAAAATGA CCTGCAGGCC 
CAGGCACGAT GTCATCGAAT TGGGCAGAGC AAAGCTGTGA AGGTGTACCG CCTCATCACT 
CGTAATTCCT ACGAGAGAGA GATGTTTGAT AAGGCCAGCC TCAAGTTGGG GTTGGATAAG 
GCTGTGCTTC AATCCATGAG TGGTCGGGAT GGCAACATTA CTGGAATCCA ACAGTTCTCT 
AAGAAGGAGA TTGAAGATCT TTTAAGAAAA GGAGCATATG CAGCCATCAT GGAGGAAGAT 
GATGAAGGCT CCAAGTTTTG TGAAGAGGAC ATTGACCAGA TCTTGTTAAG ACGAACTACA 
ACCATCACCA TTGAATCTGA AGGAAAAGGT TCCACCTTTG CTAAGGCAAG CTTTGTTGCT 
TCTGAAAACA GGACAGATAT TTCTTTGGAT GACCCCAACT TTTGGCAAAA GTGGGCCAAA 
AAGGCTGACC TAGACATGGA TCTGCTCAAC AGCAAGAATA ATTTGGTAAT TGACACACCT 
AGAGTACGAA AACAAACGCG CCACTTTAGC ACTCTGAAAG ATGATGACCT GGTGGAATTC 
TCTGATTTGG AAAGTGAGGA TGATGAGCGG CCACGCTCCC GCAGACATGA CCGTCATCAT 
GCCTATGGGC GCACTGACTG CTTTCGGGTG GAAAAGCATC TCCTGGTATA TGGTTGGGGA 
CGATGGCGAG ATATTTTATC TCATGGACGC TTCAAGCGAC GTATGACTGA ACGAGATGTG 
GAGACCATTT GTCGGGCCAT TCTCGTGTAC TGTCTTCTAC ACTACCGTGG GGATGAAAAT 
ATTAAAGGCT TCATCTGGGA CTTGATTAGC CCAGCTGAAA ATGGCAAGAC AAAAGAATTG 
CAGAATCATT CAGGTCTATC TATCCCTGTG CCTCGTGGAC GCAAAGGAAA AAAAGTAAAG 
TCACAAAGCA CTTTTGATAT CCATAAGGCA GATTGGATCC GGAAATATAA CCCTGACACT 
TTGTTCCAAG ATGAAAGTTA TAAGAAGCAC TTGAAACATC AGTGTAACAA GGTACTGTTG 
CGGGTACGAA TGCTATACTA CCTGAGGCAG GAGGTTATTG GAGACCAAGC AGAAAAGGTG 
TTAGGGGGTG CGATTGCCAG TGAGATTGAC ATATGGTTCC CAGTAGTGGA TCAACTGGAG 
GTTCCAACAA CTTGGTGGGA CAGTGAGGCT GACAAGTCGC TGCTCATTGG AGTCTTTAAA 
CATGGCTATG AGAAATATAA TACCATGAGG GCAGACCCAG CCTTATGTTT CCTAGAAAAG 
GCTGGCCGAC CAGATGACAA AGCAATTGCA GCAGAACATC GAGTGTTGGA TAACTTCTCT 
GACATAGTAG AAGGGGTTGA CTTTGATAAA GATTGTGAAG ATCCTGAATA TAAACCACTC 
CAAGGTCCCC CAAAGGACCA AGATGATGAG GGTGATCCCT TGATGATGAT GGATGAGGAG 
ATCTCAGTGA TTGATGGAGA TGAAGCCCAG GTGACCCAAC AGCCAGGCCA TTTATTCTGG 
CCTCCGGGCT CTGCCCTAAC AGCTAGGCTT CGGCGTCTAG TAACAGCGTA TCAGCGCAGC 
TACAAGAGAG AACAAATGAA GATAGAGGCT GCAGAACGTG GGGACCGGCG AAGGCGGCGT 
TGTGAAGCAG CCTTCAAGCT GAAAGAAATT GCACGGCGGG AGAAACAACA ACGATGGACA 
AGGCGTGAAC AAACTGATTT TTATCGAGTG GTGTCTACGT TTGGTGTGGA ATATGACCCT 
GACACCATGC AGTTCCATTG GGATCGCTTC CGCACTTTTG CTCGACTAGA CAAAAAGACA 
GATGAAAGCC TTACCAAGTA CTTCCATGGC TTTGTGGCCA TGTGCCGCCA AGTATGCCGC 
CTTCCCCCAG CAGCTGGAGA TGAACCCCCC GACCCTAACC TGTTCATTGA GCCCATCACT 
GAGGAGAGAG CCTCACGGAC TCTCTACCGT ATAGAATTGC TTCGGCGCTT ACGGGAACAA 
GTTTTATGCC ACCCCCTTTT GGAAGATCGG CTGGCATTGT GTCAGCCTCC TGGTCCTGAA 
TTGCCCAAAT GGTGGGAGCC TGTTCGGCAT GATGGGGAGC TTCTAAGAGG GGCAGCCCGC 
CATGGGGTGA GCCAAACAGA CTGCAACATC ATGCAGGACC CAGACTTCTC TTTTCTGGCT 
GCCCGTATGA ATTATATGCA GAACCATCAA GCAGGAGCAC CAGCTCCATC CTTGTCACGC 
TGCTCTACTC CACTGCTGCA CCAGCAGTAT ACCTCACGCA CTGCCTCACC ACTGCCCCTG 
CGCCCAGATG CTCCTGTTGA AAAGTCACCC GAGGAGACAG CTACCCAGGT CCCCAGTCTG 
GAGAGTCTGA CTTTAAAGCT AGAGCACGAG GTGGTGGCCA GGAGCCGACC AACCCCACAA 
GACTATGAGA TGCGAGTATC CCCCTCTGAT ACTACCCCTC TGGTTTCCCG GAGTGTTCCA 
CCAGTCAAAC TGGAGGATGA GGATGATTCG GACTCTGAGC TGGACTTGAG CAAGCTGTCA 
CCATCTTCTT CTTCTTCCTC ATCCTCATCC AGCTCCAGCT CCAGCACTGA TGAGAGTGAG 
GATGAGAAGG AAGAGAAGCT AACTGACCAG TCCCGCTCAA AGCTCTATGA TGAAGAGAGT 
CTCCTGTCCC TCACTATGTC CCAAGATGGA TTCCCAAATG AAGATGGAGA ACAAATGACC 
CCTGAGCTTC TGCTACTGCA GGAAAGACAA AGAGCCTCTG AGTGGCCCAA GGATCGTGTC 
CTGATAAACC GTATTGACCT CGTCTGCCAG GCTGTACTCT CAGGGAAGTG GCCTTCTAGC 
CGTAGGAGCC AGGAAATGGT AACAGGAGGA ATTTTGGGGC CAGGCAACCA CTTGCTAGAC 
AGTCCCTCAT TGACTCCTGG AGAATATGGT GACTCTCCAG TCCCCACACC ACGAAGTAGT 
AGTGCAGCTT CCATGGCAGA GGAGGAAGCA TCTGCAGTCA GCACAGCGGC AGCCCAGTTC 
ACCAAACTTC GCCGAGGCAT GGATGAAAAG GAGTTTACAG TTCAAATCAA AGATGAGGAA 
GGATTGAAGT TAACATTCCA GAAGCACAAG TTGATGGCGA ATGGAGTAAT GGGAGATGGA 
CATCCACTGT TTCATAAGAA GAAGGGGAAC AGAAAGAAGC TAGTAGAGCT GGAGGTGGAG 
TGCATGGAAG AGCCTAATCA CCTTGATGTG GACCTGGAGA CCCGGATCCC TGTCATCAAT 
AAGGTGGATG GTACTTTGCT GGTGGGTGAG GATGCCCCTC GCCGGGCTGA ACTGGAGATG 
TGGTTACAGG GTCATCCAGA GTTTGCTGTT GATCCCCGAT TTCTAGCGTA TATGGAGGAT 
CGCAGAAAAC AGAAGTGGCA AAGATGTAAA AAAAATAATA AGGCAGAATT GAACTGTTTG 
GGAATGGAAC CAGTACAGAC AGCTAACTCT AGAAATGGGA AAAAGGGTCA TCACACTGAA 
ACGGTGTTCA ACCGGGTTTT GCCAGGGCCT ATTGCACCAG AGAGCAGCAA GAAGCGGGCC 
CGTAGGATGC GACCAGACCT TTCTAAGATG ATGGCCCTCA TGCAGGGTGG AAGCACTGGG 
TCTCTATCTC TGCATAACAC GTTCCAACAC AGCAGTAGTG GCCTACAGTC TGTGTCATCT 
TTGGGTCACA GCAGTGCCAC TTCTGCATCT TTGCCTTTTA TGCCATTTGT GATGGGTGGT 
GCACCATCAT CCCCTCATGT AGACTCCAGC ACCATGCTTC ATCACCACCA CCACCACCCC 
CACCCCCACC ATCACCACCA TCACCATCCA GGCTTGAGAG CCCCTGGCTA CCCCTCTTCA 
CCAGTGACTA CCGCCTCTGG TACTACCTTG CGGTTGCCAC CACTGCAACC TGAGGAGGAT 
GACGATGAGG ATGAAGAAGA TGATGATGAC TTATCTCAGG GCTATGATAG CTCAGAAAGG 
GACTTCTCAC TCATTGATGA TCCTATGATG CCAGCTAACT CAGACTCCAG TGAAGATGCT 
GATGACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Enriched expression of genes associated with autism spectrum disorders in human inhibitory neurons.
Translational psychiatry8(1)13(2018 Jan)
Wang P,Zhao D,Lachman HM,Zheng D

Genome-wide association study identified copy number variants associated with sporadic colorectal cancer risk.
Journal of medical genetics(2017 Oct)
Thean LF,Low YS,Lo M,Teo YY,Koh WP,Yuan JM,Chew MH,Tang CL,Cheah PY

The ATP-dependent chromatin remodeling enzymes CHD6, CHD7, and CHD8 exhibit distinct nucleosome binding and remodeling activities.
The Journal of biological chemistry292(28)11927-11936(2017 07)
Manning BJ,Yusufzai T

A systematic variant annotation approach for ranking genes associated with autism spectrum disorders.
Molecular autism744(2016)
Larsen E,Menashe I,Ziats MN,Pereanu W,Packer A,Banerjee-Basu S

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CHD8 cDNA ORF clone, Homo sapiens(human)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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