ALS2 cDNA ORF clone, Homo sapiens(human)

The following ALS2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ALS2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu16433 NM_001135745.2
Latest version!
Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $237.30
$339.00
OHu89724 XM_017004572.2
Latest version!
Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu36477 XM_006712655.3
Latest version!
Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $475.30
$679.00
OHu89723 XM_024453025.1
Latest version!
Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu89723 XM_017004571.1
Latest version!
Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OHu19615 NM_020919.4
Latest version!
Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu19615 XM_006712654.3
Latest version!
Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu36475 XM_017004569.2
Latest version!
Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu70842 XM_024453024.1
Latest version!
Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu70842 XM_011511530.2
Latest version!
Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu70843 XM_017004570.2
Latest version!
Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu36475 XM_017004569.1 Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu19615 XM_006712654.1 Homo sapiens amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu19615 NM_020919.3 Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu70843 XM_017004570.1 Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu19615 XM_006712654.2 Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu89724 XM_017004572.1 Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 10-12 $475.30
$679.00
OHu16433 NM_001135745.1 Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $237.30
$339.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu16433
    Clone ID Related Accession (Same CDS sequence) NM_001135745.2 , NM_001135745.1
    Accession Version NM_001135745.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1191bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product alsin isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AB053306.1, AK023024.1, BU623061.1 and AC007279.4. On May 31, 2019 this sequence version replaced NM_001135745.1. Transcript Variant: This variant (2) uses an internal polyA site resulting in a substantially shorter transcript with a unique 3' coding region, compared to variant 1. The encoded isoform (2), also known as the short form, is substantially shorter and has a unique C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.209388.1, SRR1660807.82203.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGGTACCTG CTCAATTTTA TAAAATAAAA
    GTGTGTCTAG AGTTGAACTG TATGGGCTTT TCTTTAGAAA CTTTAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001129217.1
    CDS118..1308
    Translation

    Target ORF information:

    RefSeq Version NM_001135745.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001135745.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGGTACCTG CTCAATTTTA TAAAATAAAA
    GTGTGTCTAG AGTTGAACTG TATGGGCTTT TCTTTAGAAA CTTTAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16433
    Clone ID Related Accession (Same CDS sequence) NM_001135745.2 , NM_001135745.1
    Accession Version NM_001135745.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1191bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-20
    Organism Homo sapiens(human)
    Product alsin isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP348546.1, AC007242.3, AB053306.1, AK023024.1 and BU623061.1. Transcript Variant: This variant (2) uses an internal polyA site resulting in a substantially shorter transcript with a unique 3' coding region, compared to variant 1. The encoded isoform (2), also known as the short form, is substantially shorter and has a unique C-terminus, compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BP348546.1, SRR1660803.137880.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGGTACCTG CTCAATTTTA TAAAATAAAA
    GTGTGTCTAG AGTTGAACTG TATGGGCTTT TCTTTAGAAA CTTTAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001129217.1
    CDS357..1547
    Translation

    Target ORF information:

    RefSeq Version NM_001135745.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001135745.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGGTACCTG CTCAATTTTA TAAAATAAAA
    GTGTGTCTAG AGTTGAACTG TATGGGCTTT TCTTTAGAAA CTTTAAAATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89724
    Clone ID Related Accession (Same CDS sequence) XM_017004572.1 , XM_017004572.2
    Accession Version XM_017004572.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product alsin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGGAGGAT TCCAGCTTCT TGCTAAGCCT GCCATTGATT TCCTAAATAA AAACCAAGAG 
    CTGTTGCAAG ATTTGTCAGA AGTGAATGAC GAAAACACTC AGTTGATGGA AATACTGAAT
    ACTTTGTTTT TCTTGCCAAT CAGACGACTT CATAATTACG CAAAAGTTTT GCTAAAGCTT
    GCTACTTGTT TTGAAGTGGC ATCTCCAGAA TATCAGAAAC TGCAGGATTC CAGTTCTTGT
    TATGAGTGTC TTGCTCTCCA TCTCGGCAGG AAAAGGAAGG AAGCAGAATA CACACTGGGC
    TTCTGGAAGA CCTTCCCCGG AAAAATGACG GATTCCTTGA GGAAGCCAGA GCGTCGACTG
    CTGTGTGAGA GTAGTAACCG AGCCCTGTCT CTGCAGCATG CTGGGAGGTT TTCCGTGAAT
    TGGTTCATTC TCTTTAATGA TGCCCTGGTC CATGCCCAGT TCTCCACGCA CCATGTTTTC
    CCTCTGGCCA CGCTGTGGGC AGAGCCACTG TCTGAAGAAG CTGGTGGTGT GAATGGCTTA
    AAGATAACTA CACCTGAGGA GCAGTTCACT CTCATTTCAT CTACACCCCA GGAAAAGACA
    AAGTGGCTAC GAGCTATAAG CCAAGCCGTA GATCAGGCTT TGAGAGGGAT GTCTGATCTC
    CCCCCTTATG GAAGTGGTAG CAGTGTTCAG AGACAGGAAC CACCCATTTC ACGCAGTGCC
    AAATATACTT TCTACAAGGA TCCTCGCCTA AAGGATGCCA CCTATGATGG ACGCTGGCTT
    TCAGGGAAGC CTCATGGCAG AGGGGTTTTG AAGTGGCCTG ATGGAAAGAT GTATTCTGGC
    ATGTTCAGGA ATGGCTTGGA AGATGGGTAT GGAGAATACA GAATCCCAAA CAAGGCAATG
    AACAAAGAAG ACCATTATGT GGGCCATTGG AAAGAAGGAA AAATGTGCGG TCAAGGAGTC
    TACAGCTATG CTTCTGGTGA AGTATTTGAG GGCTGTTTTC AAGATAATAT GCGTCATGGT
    CATGGTCTTC TACGAAGTGG GAAATTGACG TCCTCTTCTC CTAGTATGTT CATTGGCCAG
    TGGGTAATGG ATAAGAAAGC AGGATATGGT GTCTTTGATG ATATCACTAG GGGGGAAAAG
    TATATGGGAA TGTGGCAAGA TGATGTGTGT CAAGGGAATG GTGTGGTGGT TACCCAGTTT
    GGATTATACT ACGAGGGCAA CTTTCACCTT AATAAAATGA TGGGAAATGG GGTTTTGCTT
    TCCGAAGATG ATACTATCTA TGAAGGAGAA TTTTCAGATG ACTGGACTCT TAGTGGAAAG
    GGAACACTGA CTATGCCAAA TGGAGACTAC ATTGAAGGTT ATTTTAGTGG AGAATGGGGA
    TCTGGGATAA AAATCACTGG AACCTACTTC AAACCTAGTC TATATGAGAG TGATAAAGAC
    AGACCTAAAG TTTTCAGGAA GCTAGGAAAC CTGGCAGTGC CAGCTGATGA GAAGTGGAAA
    GCGGTGTTTG ACGAATGTTG GCGCCAACTG GGCTGTGAGG GCCCAGGCCA AGGGGAAGTT
    TGGAAAGCAT GGGACAATAT TGCTGTGGCC TTGACCACCA GTCGGCGCCA GCACAGAGAC
    AGTCCAGAAA TACTGAGTCG TTCACAGACT CAGACACTAG AGAGTTTGGA ATTCATTCCA
    CAGCATGTTG GTGCCTTCTC TGTGGAGAAA TATGATGACA TCAGGAAATA TTTAATAAAG
    GCCTGTGACA CTCCTCTGCA CCCCCTGGGC AGGCTTGTGG AGACACTGGT TGCAGTGTAT
    AGAATGACAT ACGTGGGCGT AGGAGCCAAC CGCAGGTTAT TGCAGGAGGC TGTAAAGGAG
    ATTAAGTCCT ATCTTAAGCG AATTTTCCAG CTGGTGAGGT TCTTATTTCC TGAGCTGCCT
    GAAGAAGGCA GCACAATTCC TCTCTCTGCT CCTCTGCCAA CCGAAAGGAA GTCTTTTTGC
    ACTGGGAAGT CAGATTCCCG ATCTGAATCA CCAGAGCCAG GTTATGTAGT AACGAGTTCT
    GGATTATTGC TTCCTGTGCT GCTACCTCGG CTCTACCCAC CGCTGTTTAT GCTTTATGCT
    TTGGATAATG ATCGCGAGGA AGACATTTAC TGGGAATGTG TCCTTCGACT AAATAAGCAG
    CCAGATATTG CTCTCCTGGG CTTTCTTGGG GTGCAGAGGA AATTTTGGCC AGCAACCTTG
    TCAATCCTTG GAGAGAGTAA AAAGGTTTTG CCAACCACGA AAGATGCTTG TTTTGCCTCA
    GCAGTAGAAT GTCTGCAGCA GATCAGCACA ACATTTACCC CATCAGACAA ACTTAAGGTC
    ATCCAGCAGA CTTTTGAGGA GATCTCTCAG AGTGTCCTGG CGTCACTCCA CGAAGACTTC
    TTGTGGTCCA TGGATGACTT GTTTCCTGTT TTCTTATATG TGGTGCTACG GGCCAGGATT
    AGGAATTTAG GCTCTGAGGT ACACCTCATT GAGGATCTAA TGGACCCCTA TCTTCAGCAT
    GGGGAACAGG GTATAATGTT CACCACCTTG AAGGCATGTT ACTACCAGAT TCAGCGTGAG
    AAGCTTAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860061.1
    CDS2031..4622
    Translation

    Target ORF information:

    RefSeq Version XM_017004572.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004572.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGGAGGAT TCCAGCTTCT TGCTAAGCCT GCCATTGATT TCCTAAATAA AAACCAAGAG 
    CTGTTGCAAG ATTTGTCAGA AGTGAATGAC GAAAACACTC AGTTGATGGA AATACTGAAT
    ACTTTGTTTT TCTTGCCAAT CAGACGACTT CATAATTACG CAAAAGTTTT GCTAAAGCTT
    GCTACTTGTT TTGAAGTGGC ATCTCCAGAA TATCAGAAAC TGCAGGATTC CAGTTCTTGT
    TATGAGTGTC TTGCTCTCCA TCTCGGCAGG AAAAGGAAGG AAGCAGAATA CACACTGGGC
    TTCTGGAAGA CCTTCCCCGG AAAAATGACG GATTCCTTGA GGAAGCCAGA GCGTCGACTG
    CTGTGTGAGA GTAGTAACCG AGCCCTGTCT CTGCAGCATG CTGGGAGGTT TTCCGTGAAT
    TGGTTCATTC TCTTTAATGA TGCCCTGGTC CATGCCCAGT TCTCCACGCA CCATGTTTTC
    CCTCTGGCCA CGCTGTGGGC AGAGCCACTG TCTGAAGAAG CTGGTGGTGT GAATGGCTTA
    AAGATAACTA CACCTGAGGA GCAGTTCACT CTCATTTCAT CTACACCCCA GGAAAAGACA
    AAGTGGCTAC GAGCTATAAG CCAAGCCGTA GATCAGGCTT TGAGAGGGAT GTCTGATCTC
    CCCCCTTATG GAAGTGGTAG CAGTGTTCAG AGACAGGAAC CACCCATTTC ACGCAGTGCC
    AAATATACTT TCTACAAGGA TCCTCGCCTA AAGGATGCCA CCTATGATGG ACGCTGGCTT
    TCAGGGAAGC CTCATGGCAG AGGGGTTTTG AAGTGGCCTG ATGGAAAGAT GTATTCTGGC
    ATGTTCAGGA ATGGCTTGGA AGATGGGTAT GGAGAATACA GAATCCCAAA CAAGGCAATG
    AACAAAGAAG ACCATTATGT GGGCCATTGG AAAGAAGGAA AAATGTGCGG TCAAGGAGTC
    TACAGCTATG CTTCTGGTGA AGTATTTGAG GGCTGTTTTC AAGATAATAT GCGTCATGGT
    CATGGTCTTC TACGAAGTGG GAAATTGACG TCCTCTTCTC CTAGTATGTT CATTGGCCAG
    TGGGTAATGG ATAAGAAAGC AGGATATGGT GTCTTTGATG ATATCACTAG GGGGGAAAAG
    TATATGGGAA TGTGGCAAGA TGATGTGTGT CAAGGGAATG GTGTGGTGGT TACCCAGTTT
    GGATTATACT ACGAGGGCAA CTTTCACCTT AATAAAATGA TGGGAAATGG GGTTTTGCTT
    TCCGAAGATG ATACTATCTA TGAAGGAGAA TTTTCAGATG ACTGGACTCT TAGTGGAAAG
    GGAACACTGA CTATGCCAAA TGGAGACTAC ATTGAAGGTT ATTTTAGTGG AGAATGGGGA
    TCTGGGATAA AAATCACTGG AACCTACTTC AAACCTAGTC TATATGAGAG TGATAAAGAC
    AGACCTAAAG TTTTCAGGAA GCTAGGAAAC CTGGCAGTGC CAGCTGATGA GAAGTGGAAA
    GCGGTGTTTG ACGAATGTTG GCGCCAACTG GGCTGTGAGG GCCCAGGCCA AGGGGAAGTT
    TGGAAAGCAT GGGACAATAT TGCTGTGGCC TTGACCACCA GTCGGCGCCA GCACAGAGAC
    AGTCCAGAAA TACTGAGTCG TTCACAGACT CAGACACTAG AGAGTTTGGA ATTCATTCCA
    CAGCATGTTG GTGCCTTCTC TGTGGAGAAA TATGATGACA TCAGGAAATA TTTAATAAAG
    GCCTGTGACA CTCCTCTGCA CCCCCTGGGC AGGCTTGTGG AGACACTGGT TGCAGTGTAT
    AGAATGACAT ACGTGGGCGT AGGAGCCAAC CGCAGGTTAT TGCAGGAGGC TGTAAAGGAG
    ATTAAGTCCT ATCTTAAGCG AATTTTCCAG CTGGTGAGGT TCTTATTTCC TGAGCTGCCT
    GAAGAAGGCA GCACAATTCC TCTCTCTGCT CCTCTGCCAA CCGAAAGGAA GTCTTTTTGC
    ACTGGGAAGT CAGATTCCCG ATCTGAATCA CCAGAGCCAG GTTATGTAGT AACGAGTTCT
    GGATTATTGC TTCCTGTGCT GCTACCTCGG CTCTACCCAC CGCTGTTTAT GCTTTATGCT
    TTGGATAATG ATCGCGAGGA AGACATTTAC TGGGAATGTG TCCTTCGACT AAATAAGCAG
    CCAGATATTG CTCTCCTGGG CTTTCTTGGG GTGCAGAGGA AATTTTGGCC AGCAACCTTG
    TCAATCCTTG GAGAGAGTAA AAAGGTTTTG CCAACCACGA AAGATGCTTG TTTTGCCTCA
    GCAGTAGAAT GTCTGCAGCA GATCAGCACA ACATTTACCC CATCAGACAA ACTTAAGGTC
    ATCCAGCAGA CTTTTGAGGA GATCTCTCAG AGTGTCCTGG CGTCACTCCA CGAAGACTTC
    TTGTGGTCCA TGGATGACTT GTTTCCTGTT TTCTTATATG TGGTGCTACG GGCCAGGATT
    AGGAATTTAG GCTCTGAGGT ACACCTCATT GAGGATCTAA TGGACCCCTA TCTTCAGCAT
    GGGGAACAGG GTATAATGTT CACCACCTTG AAGGCATGTT ACTACCAGAT TCAGCGTGAG
    AAGCTTAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89724
    Clone ID Related Accession (Same CDS sequence) XM_017004572.1 , XM_017004572.2
    Accession Version XM_017004572.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2592bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alsin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017004572.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGGAGGAT TCCAGCTTCT TGCTAAGCCT GCCATTGATT TCCTAAATAA AAACCAAGAG 
    CTGTTGCAAG ATTTGTCAGA AGTGAATGAC GAAAACACTC AGTTGATGGA AATACTGAAT
    ACTTTGTTTT TCTTGCCAAT CAGACGACTT CATAATTACG CAAAAGTTTT GCTAAAGCTT
    GCTACTTGTT TTGAAGTGGC ATCTCCAGAA TATCAGAAAC TGCAGGATTC CAGTTCTTGT
    TATGAGTGTC TTGCTCTCCA TCTCGGCAGG AAAAGGAAGG AAGCAGAATA CACACTGGGC
    TTCTGGAAGA CCTTCCCCGG AAAAATGACG GATTCCTTGA GGAAGCCAGA GCGTCGACTG
    CTGTGTGAGA GTAGTAACCG AGCCCTGTCT CTGCAGCATG CTGGGAGGTT TTCCGTGAAT
    TGGTTCATTC TCTTTAATGA TGCCCTGGTC CATGCCCAGT TCTCCACGCA CCATGTTTTC
    CCTCTGGCCA CGCTGTGGGC AGAGCCACTG TCTGAAGAAG CTGGTGGTGT GAATGGCTTA
    AAGATAACTA CACCTGAGGA GCAGTTCACT CTCATTTCAT CTACACCCCA GGAAAAGACA
    AAGTGGCTAC GAGCTATAAG CCAAGCCGTA GATCAGGCTT TGAGAGGGAT GTCTGATCTC
    CCCCCTTATG GAAGTGGTAG CAGTGTTCAG AGACAGGAAC CACCCATTTC ACGCAGTGCC
    AAATATACTT TCTACAAGGA TCCTCGCCTA AAGGATGCCA CCTATGATGG ACGCTGGCTT
    TCAGGGAAGC CTCATGGCAG AGGGGTTTTG AAGTGGCCTG ATGGAAAGAT GTATTCTGGC
    ATGTTCAGGA ATGGCTTGGA AGATGGGTAT GGAGAATACA GAATCCCAAA CAAGGCAATG
    AACAAAGAAG ACCATTATGT GGGCCATTGG AAAGAAGGAA AAATGTGCGG TCAAGGAGTC
    TACAGCTATG CTTCTGGTGA AGTATTTGAG GGCTGTTTTC AAGATAATAT GCGTCATGGT
    CATGGTCTTC TACGAAGTGG GAAATTGACG TCCTCTTCTC CTAGTATGTT CATTGGCCAG
    TGGGTAATGG ATAAGAAAGC AGGATATGGT GTCTTTGATG ATATCACTAG GGGGGAAAAG
    TATATGGGAA TGTGGCAAGA TGATGTGTGT CAAGGGAATG GTGTGGTGGT TACCCAGTTT
    GGATTATACT ACGAGGGCAA CTTTCACCTT AATAAAATGA TGGGAAATGG GGTTTTGCTT
    TCCGAAGATG ATACTATCTA TGAAGGAGAA TTTTCAGATG ACTGGACTCT TAGTGGAAAG
    GGAACACTGA CTATGCCAAA TGGAGACTAC ATTGAAGGTT ATTTTAGTGG AGAATGGGGA
    TCTGGGATAA AAATCACTGG AACCTACTTC AAACCTAGTC TATATGAGAG TGATAAAGAC
    AGACCTAAAG TTTTCAGGAA GCTAGGAAAC CTGGCAGTGC CAGCTGATGA GAAGTGGAAA
    GCGGTGTTTG ACGAATGTTG GCGCCAACTG GGCTGTGAGG GCCCAGGCCA AGGGGAAGTT
    TGGAAAGCAT GGGACAATAT TGCTGTGGCC TTGACCACCA GTCGGCGCCA GCACAGAGAC
    AGTCCAGAAA TACTGAGTCG TTCACAGACT CAGACACTAG AGAGTTTGGA ATTCATTCCA
    CAGCATGTTG GTGCCTTCTC TGTGGAGAAA TATGATGACA TCAGGAAATA TTTAATAAAG
    GCCTGTGACA CTCCTCTGCA CCCCCTGGGC AGGCTTGTGG AGACACTGGT TGCAGTGTAT
    AGAATGACAT ACGTGGGCGT AGGAGCCAAC CGCAGGTTAT TGCAGGAGGC TGTAAAGGAG
    ATTAAGTCCT ATCTTAAGCG AATTTTCCAG CTGGTGAGGT TCTTATTTCC TGAGCTGCCT
    GAAGAAGGCA GCACAATTCC TCTCTCTGCT CCTCTGCCAA CCGAAAGGAA GTCTTTTTGC
    ACTGGGAAGT CAGATTCCCG ATCTGAATCA CCAGAGCCAG GTTATGTAGT AACGAGTTCT
    GGATTATTGC TTCCTGTGCT GCTACCTCGG CTCTACCCAC CGCTGTTTAT GCTTTATGCT
    TTGGATAATG ATCGCGAGGA AGACATTTAC TGGGAATGTG TCCTTCGACT AAATAAGCAG
    CCAGATATTG CTCTCCTGGG CTTTCTTGGG GTGCAGAGGA AATTTTGGCC AGCAACCTTG
    TCAATCCTTG GAGAGAGTAA AAAGGTTTTG CCAACCACGA AAGATGCTTG TTTTGCCTCA
    GCAGTAGAAT GTCTGCAGCA GATCAGCACA ACATTTACCC CATCAGACAA ACTTAAGGTC
    ATCCAGCAGA CTTTTGAGGA GATCTCTCAG AGTGTCCTGG CGTCACTCCA CGAAGACTTC
    TTGTGGTCCA TGGATGACTT GTTTCCTGTT TTCTTATATG TGGTGCTACG GGCCAGGATT
    AGGAATTTAG GCTCTGAGGT ACACCTCATT GAGGATCTAA TGGACCCCTA TCTTCAGCAT
    GGGGAACAGG GTATAATGTT CACCACCTTG AAGGCATGTT ACTACCAGAT TCAGCGTGAG
    AAGCTTAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860061.1
    CDS1739..4330
    Translation

    Target ORF information:

    RefSeq Version XM_017004572.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004572.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGGGAGGAT TCCAGCTTCT TGCTAAGCCT GCCATTGATT TCCTAAATAA AAACCAAGAG 
    CTGTTGCAAG ATTTGTCAGA AGTGAATGAC GAAAACACTC AGTTGATGGA AATACTGAAT
    ACTTTGTTTT TCTTGCCAAT CAGACGACTT CATAATTACG CAAAAGTTTT GCTAAAGCTT
    GCTACTTGTT TTGAAGTGGC ATCTCCAGAA TATCAGAAAC TGCAGGATTC CAGTTCTTGT
    TATGAGTGTC TTGCTCTCCA TCTCGGCAGG AAAAGGAAGG AAGCAGAATA CACACTGGGC
    TTCTGGAAGA CCTTCCCCGG AAAAATGACG GATTCCTTGA GGAAGCCAGA GCGTCGACTG
    CTGTGTGAGA GTAGTAACCG AGCCCTGTCT CTGCAGCATG CTGGGAGGTT TTCCGTGAAT
    TGGTTCATTC TCTTTAATGA TGCCCTGGTC CATGCCCAGT TCTCCACGCA CCATGTTTTC
    CCTCTGGCCA CGCTGTGGGC AGAGCCACTG TCTGAAGAAG CTGGTGGTGT GAATGGCTTA
    AAGATAACTA CACCTGAGGA GCAGTTCACT CTCATTTCAT CTACACCCCA GGAAAAGACA
    AAGTGGCTAC GAGCTATAAG CCAAGCCGTA GATCAGGCTT TGAGAGGGAT GTCTGATCTC
    CCCCCTTATG GAAGTGGTAG CAGTGTTCAG AGACAGGAAC CACCCATTTC ACGCAGTGCC
    AAATATACTT TCTACAAGGA TCCTCGCCTA AAGGATGCCA CCTATGATGG ACGCTGGCTT
    TCAGGGAAGC CTCATGGCAG AGGGGTTTTG AAGTGGCCTG ATGGAAAGAT GTATTCTGGC
    ATGTTCAGGA ATGGCTTGGA AGATGGGTAT GGAGAATACA GAATCCCAAA CAAGGCAATG
    AACAAAGAAG ACCATTATGT GGGCCATTGG AAAGAAGGAA AAATGTGCGG TCAAGGAGTC
    TACAGCTATG CTTCTGGTGA AGTATTTGAG GGCTGTTTTC AAGATAATAT GCGTCATGGT
    CATGGTCTTC TACGAAGTGG GAAATTGACG TCCTCTTCTC CTAGTATGTT CATTGGCCAG
    TGGGTAATGG ATAAGAAAGC AGGATATGGT GTCTTTGATG ATATCACTAG GGGGGAAAAG
    TATATGGGAA TGTGGCAAGA TGATGTGTGT CAAGGGAATG GTGTGGTGGT TACCCAGTTT
    GGATTATACT ACGAGGGCAA CTTTCACCTT AATAAAATGA TGGGAAATGG GGTTTTGCTT
    TCCGAAGATG ATACTATCTA TGAAGGAGAA TTTTCAGATG ACTGGACTCT TAGTGGAAAG
    GGAACACTGA CTATGCCAAA TGGAGACTAC ATTGAAGGTT ATTTTAGTGG AGAATGGGGA
    TCTGGGATAA AAATCACTGG AACCTACTTC AAACCTAGTC TATATGAGAG TGATAAAGAC
    AGACCTAAAG TTTTCAGGAA GCTAGGAAAC CTGGCAGTGC CAGCTGATGA GAAGTGGAAA
    GCGGTGTTTG ACGAATGTTG GCGCCAACTG GGCTGTGAGG GCCCAGGCCA AGGGGAAGTT
    TGGAAAGCAT GGGACAATAT TGCTGTGGCC TTGACCACCA GTCGGCGCCA GCACAGAGAC
    AGTCCAGAAA TACTGAGTCG TTCACAGACT CAGACACTAG AGAGTTTGGA ATTCATTCCA
    CAGCATGTTG GTGCCTTCTC TGTGGAGAAA TATGATGACA TCAGGAAATA TTTAATAAAG
    GCCTGTGACA CTCCTCTGCA CCCCCTGGGC AGGCTTGTGG AGACACTGGT TGCAGTGTAT
    AGAATGACAT ACGTGGGCGT AGGAGCCAAC CGCAGGTTAT TGCAGGAGGC TGTAAAGGAG
    ATTAAGTCCT ATCTTAAGCG AATTTTCCAG CTGGTGAGGT TCTTATTTCC TGAGCTGCCT
    GAAGAAGGCA GCACAATTCC TCTCTCTGCT CCTCTGCCAA CCGAAAGGAA GTCTTTTTGC
    ACTGGGAAGT CAGATTCCCG ATCTGAATCA CCAGAGCCAG GTTATGTAGT AACGAGTTCT
    GGATTATTGC TTCCTGTGCT GCTACCTCGG CTCTACCCAC CGCTGTTTAT GCTTTATGCT
    TTGGATAATG ATCGCGAGGA AGACATTTAC TGGGAATGTG TCCTTCGACT AAATAAGCAG
    CCAGATATTG CTCTCCTGGG CTTTCTTGGG GTGCAGAGGA AATTTTGGCC AGCAACCTTG
    TCAATCCTTG GAGAGAGTAA AAAGGTTTTG CCAACCACGA AAGATGCTTG TTTTGCCTCA
    GCAGTAGAAT GTCTGCAGCA GATCAGCACA ACATTTACCC CATCAGACAA ACTTAAGGTC
    ATCCAGCAGA CTTTTGAGGA GATCTCTCAG AGTGTCCTGG CGTCACTCCA CGAAGACTTC
    TTGTGGTCCA TGGATGACTT GTTTCCTGTT TTCTTATATG TGGTGCTACG GGCCAGGATT
    AGGAATTTAG GCTCTGAGGT ACACCTCATT GAGGATCTAA TGGACCCCTA TCTTCAGCAT
    GGGGAACAGG GTATAATGTT CACCACCTTG AAGGCATGTT ACTACCAGAT TCAGCGTGAG
    AAGCTTAACT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36477
    Clone ID Related Accession (Same CDS sequence) XM_006712655.3
    Accession Version XM_006712655.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2910bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alsin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712655.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGTATA TTGCCAGTCT CCACGAGTTA GCTACTACAG AAAGACGATT CTATTCAAAA 
    CTAAGTGATA TCAAATCTCA GATTCTCAGG CCTCTTCTCA GTTTAGAAAA TTTGGGCACT
    ACAACTACAG TCCAGCTGTT GCAGGAGGTG GCTAGCCGAT TCAGCAAGCT GTGTTACCTC
    ATTGGTCAGC ATGGAGCCTC ATTGAGCAGC TTCCTTCATG GGGTAAAGGA AGCCAGGAGT
    TTGGTCATCC TGAAGCATTC AAGTCTCTTC TTGGATAGTT ATACAGAGTA TTGCACATCT
    ATTACAAATT TCCTGGTTAT GGGAGGATTC CAGCTTCTTG CTAAGCCTGC CATTGATTTC
    CTAAATAAAA ACCAAGAGCT GTTGCAAGAT TTGTCAGAAG TGAATGACGA AAACACTCAG
    TTGATGGAAA TACTGAATAC TTTGTTTTTC TTGCCAATCA GACGACTTCA TAATTACGCA
    AAAGTTTTGC TAAAGCTTGC TACTTGTTTT GAAGTGGCAT CTCCAGAATA TCAGAAACTG
    CAGGATTCCA GTTCTTGTTA TGAGTGTCTT GCTCTCCATC TCGGCAGGAA AAGGAAGGAA
    GCAGAATACA CACTGGGCTT CTGGAAGACC TTCCCCGGAA AAATGACGGA TTCCTTGAGG
    AAGCCAGAGC GTCGACTGCT GTGTGAGAGT AGTAACCGAG CCCTGTCTCT GCAGCATGCT
    GGGAGGTTTT CCGTGAATTG GTTCATTCTC TTTAATGATG CCCTGGTCCA TGCCCAGTTC
    TCCACGCACC ATGTTTTCCC TCTGGCCACG CTGTGGGCAG AGCCACTGTC TGAAGAAGCT
    GGTGGTGTGA ATGGCTTAAA GATAACTACA CCTGAGGAGC AGTTCACTCT CATTTCATCT
    ACACCCCAGG AAAAGACAAA GTGGCTACGA GCTATAAGCC AAGCCGTAGA TCAGGCTTTG
    AGAGGGATGT CTGATCTCCC CCCTTATGGA AGTGGTAGCA GTGTTCAGAG ACAGGAACCA
    CCCATTTCAC GCAGTGCCAA ATATACTTTC TACAAGGATC CTCGCCTAAA GGATGCCACC
    TATGATGGAC GCTGGCTTTC AGGGAAGCCT CATGGCAGAG GGGTTTTGAA GTGGCCTGAT
    GGAAAGATGT ATTCTGGCAT GTTCAGGAAT GGCTTGGAAG ATGGGTATGG AGAATACAGA
    ATCCCAAACA AGGCAATGAA CAAAGAAGAC CATTATGTGG GCCATTGGAA AGAAGGAAAA
    ATGTGCGGTC AAGGAGTCTA CAGCTATGCT TCTGGTGAAG TATTTGAGGG CTGTTTTCAA
    GATAATATGC GTCATGGTCA TGGTCTTCTA CGAAGTGGGA AATTGACGTC CTCTTCTCCT
    AGTATGTTCA TTGGCCAGTG GGTAATGGAT AAGAAAGCAG GATATGGTGT CTTTGATGAT
    ATCACTAGGG GGGAAAAGTA TATGGGAATG TGGCAAGATG ATGTGTGTCA AGGGAATGGT
    GTGGTGGTTA CCCAGTTTGG ATTATACTAC GAGGGCAACT TTCACCTTAA TAAAATGATG
    GGAAATGGGG TTTTGCTTTC CGAAGATGAT ACTATCTATG AAGGAGAATT TTCAGATGAC
    TGGACTCTTA GTGGAAAGGG AACACTGACT ATGCCAAATG GAGACTACAT TGAAGGTTAT
    TTTAGTGGAG AATGGGGATC TGGGATAAAA ATCACTGGAA CCTACTTCAA ACCTAGTCTA
    TATGAGAGTG ATAAAGACAG ACCTAAAGTT TTCAGGAAGC TAGGAAACCT GGCAGTGCCA
    GCTGATGAGA AGTGGAAAGC GGTGTTTGAC GAATGTTGGC GCCAACTGGG CTGTGAGGGC
    CCAGGCCAAG GGGAAGTTTG GAAAGCATGG GACAATATTG CTGTGGCCTT GACCACCAGT
    CGGCGCCAGC ACAGAGACAG TCCAGAAATA CTGAGTCGTT CACAGACTCA GACACTAGAG
    AGTTTGGAAT TCATTCCACA GCATGTTGGT GCCTTCTCTG TGGAGAAATA TGATGACATC
    AGGAAATATT TAATAAAGGC CTGTGACACT CCTCTGCACC CCCTGGGCAG GCTTGTGGAG
    ACACTGGTTG CAGTGTATAG AATGACATAC GTGGGCGTAG GAGCCAACCG CAGGTTATTG
    CAGGAGGCTG TAAAGGAGAT TAAGTCCTAT CTTAAGCGAA TTTTCCAGCT GGTGAGGTTC
    TTATTTCCTG AGCTGCCTGA AGAAGGCAGC ACAATTCCTC TCTCTGCTCC TCTGCCAACC
    GAAAGGAAGT CTTTTTGCAC TGGGAAGTCA GATTCCCGAT CTGAATCACC AGAGCCAGGT
    TATGTAGTAA CGAGTTCTGG ATTATTGCTT CCTGTGCTGC TACCTCGGCT CTACCCACCG
    CTGTTTATGC TTTATGCTTT GGATAATGAT CGCGAGGAAG ACATTTACTG GGAATGTGTC
    CTTCGACTAA ATAAGCAGCC AGATATTGCT CTCCTGGGCT TTCTTGGGGT GCAGAGGAAA
    TTTTGGCCAG CAACCTTGTC AATCCTTGGA GAGAGTAAAA AGGTTTTGCC AACCACGAAA
    GATGCTTGTT TTGCCTCAGC AGTAGAATGT CTGCAGCAGA TCAGCACAAC ATTTACCCCA
    TCAGACAAAC TTAAGGTCAT CCAGCAGACT TTTGAGGAGA TCTCTCAGAG TGTCCTGGCG
    TCACTCCACG AAGACTTCTT GTGGTCCATG GATGACTTGT TTCCTGTTTT CTTATATGTG
    GTGCTACGGG CCAGGATTAG GAATTTAGGC TCTGAGGTAC ACCTCATTGA GGATCTAATG
    GACCCCTATC TTCAGCATGG GGAACAGGGT ATAATGTTCA CCACCTTGAA GGCATGTTAC
    TACCAGATTC AGCGTGAGAA GCTTAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712718.1
    CDS635..3544
    Translation

    Target ORF information:

    RefSeq Version XM_006712655.3
    Organism Homo sapiens(human)
    Definition Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712655.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGTATA TTGCCAGTCT CCACGAGTTA GCTACTACAG AAAGACGATT CTATTCAAAA 
    CTAAGTGATA TCAAATCTCA GATTCTCAGG CCTCTTCTCA GTTTAGAAAA TTTGGGCACT
    ACAACTACAG TCCAGCTGTT GCAGGAGGTG GCTAGCCGAT TCAGCAAGCT GTGTTACCTC
    ATTGGTCAGC ATGGAGCCTC ATTGAGCAGC TTCCTTCATG GGGTAAAGGA AGCCAGGAGT
    TTGGTCATCC TGAAGCATTC AAGTCTCTTC TTGGATAGTT ATACAGAGTA TTGCACATCT
    ATTACAAATT TCCTGGTTAT GGGAGGATTC CAGCTTCTTG CTAAGCCTGC CATTGATTTC
    CTAAATAAAA ACCAAGAGCT GTTGCAAGAT TTGTCAGAAG TGAATGACGA AAACACTCAG
    TTGATGGAAA TACTGAATAC TTTGTTTTTC TTGCCAATCA GACGACTTCA TAATTACGCA
    AAAGTTTTGC TAAAGCTTGC TACTTGTTTT GAAGTGGCAT CTCCAGAATA TCAGAAACTG
    CAGGATTCCA GTTCTTGTTA TGAGTGTCTT GCTCTCCATC TCGGCAGGAA AAGGAAGGAA
    GCAGAATACA CACTGGGCTT CTGGAAGACC TTCCCCGGAA AAATGACGGA TTCCTTGAGG
    AAGCCAGAGC GTCGACTGCT GTGTGAGAGT AGTAACCGAG CCCTGTCTCT GCAGCATGCT
    GGGAGGTTTT CCGTGAATTG GTTCATTCTC TTTAATGATG CCCTGGTCCA TGCCCAGTTC
    TCCACGCACC ATGTTTTCCC TCTGGCCACG CTGTGGGCAG AGCCACTGTC TGAAGAAGCT
    GGTGGTGTGA ATGGCTTAAA GATAACTACA CCTGAGGAGC AGTTCACTCT CATTTCATCT
    ACACCCCAGG AAAAGACAAA GTGGCTACGA GCTATAAGCC AAGCCGTAGA TCAGGCTTTG
    AGAGGGATGT CTGATCTCCC CCCTTATGGA AGTGGTAGCA GTGTTCAGAG ACAGGAACCA
    CCCATTTCAC GCAGTGCCAA ATATACTTTC TACAAGGATC CTCGCCTAAA GGATGCCACC
    TATGATGGAC GCTGGCTTTC AGGGAAGCCT CATGGCAGAG GGGTTTTGAA GTGGCCTGAT
    GGAAAGATGT ATTCTGGCAT GTTCAGGAAT GGCTTGGAAG ATGGGTATGG AGAATACAGA
    ATCCCAAACA AGGCAATGAA CAAAGAAGAC CATTATGTGG GCCATTGGAA AGAAGGAAAA
    ATGTGCGGTC AAGGAGTCTA CAGCTATGCT TCTGGTGAAG TATTTGAGGG CTGTTTTCAA
    GATAATATGC GTCATGGTCA TGGTCTTCTA CGAAGTGGGA AATTGACGTC CTCTTCTCCT
    AGTATGTTCA TTGGCCAGTG GGTAATGGAT AAGAAAGCAG GATATGGTGT CTTTGATGAT
    ATCACTAGGG GGGAAAAGTA TATGGGAATG TGGCAAGATG ATGTGTGTCA AGGGAATGGT
    GTGGTGGTTA CCCAGTTTGG ATTATACTAC GAGGGCAACT TTCACCTTAA TAAAATGATG
    GGAAATGGGG TTTTGCTTTC CGAAGATGAT ACTATCTATG AAGGAGAATT TTCAGATGAC
    TGGACTCTTA GTGGAAAGGG AACACTGACT ATGCCAAATG GAGACTACAT TGAAGGTTAT
    TTTAGTGGAG AATGGGGATC TGGGATAAAA ATCACTGGAA CCTACTTCAA ACCTAGTCTA
    TATGAGAGTG ATAAAGACAG ACCTAAAGTT TTCAGGAAGC TAGGAAACCT GGCAGTGCCA
    GCTGATGAGA AGTGGAAAGC GGTGTTTGAC GAATGTTGGC GCCAACTGGG CTGTGAGGGC
    CCAGGCCAAG GGGAAGTTTG GAAAGCATGG GACAATATTG CTGTGGCCTT GACCACCAGT
    CGGCGCCAGC ACAGAGACAG TCCAGAAATA CTGAGTCGTT CACAGACTCA GACACTAGAG
    AGTTTGGAAT TCATTCCACA GCATGTTGGT GCCTTCTCTG TGGAGAAATA TGATGACATC
    AGGAAATATT TAATAAAGGC CTGTGACACT CCTCTGCACC CCCTGGGCAG GCTTGTGGAG
    ACACTGGTTG CAGTGTATAG AATGACATAC GTGGGCGTAG GAGCCAACCG CAGGTTATTG
    CAGGAGGCTG TAAAGGAGAT TAAGTCCTAT CTTAAGCGAA TTTTCCAGCT GGTGAGGTTC
    TTATTTCCTG AGCTGCCTGA AGAAGGCAGC ACAATTCCTC TCTCTGCTCC TCTGCCAACC
    GAAAGGAAGT CTTTTTGCAC TGGGAAGTCA GATTCCCGAT CTGAATCACC AGAGCCAGGT
    TATGTAGTAA CGAGTTCTGG ATTATTGCTT CCTGTGCTGC TACCTCGGCT CTACCCACCG
    CTGTTTATGC TTTATGCTTT GGATAATGAT CGCGAGGAAG ACATTTACTG GGAATGTGTC
    CTTCGACTAA ATAAGCAGCC AGATATTGCT CTCCTGGGCT TTCTTGGGGT GCAGAGGAAA
    TTTTGGCCAG CAACCTTGTC AATCCTTGGA GAGAGTAAAA AGGTTTTGCC AACCACGAAA
    GATGCTTGTT TTGCCTCAGC AGTAGAATGT CTGCAGCAGA TCAGCACAAC ATTTACCCCA
    TCAGACAAAC TTAAGGTCAT CCAGCAGACT TTTGAGGAGA TCTCTCAGAG TGTCCTGGCG
    TCACTCCACG AAGACTTCTT GTGGTCCATG GATGACTTGT TTCCTGTTTT CTTATATGTG
    GTGCTACGGG CCAGGATTAG GAATTTAGGC TCTGAGGTAC ACCTCATTGA GGATCTAATG
    GACCCCTATC TTCAGCATGG GGAACAGGGT ATAATGTTCA CCACCTTGAA GGCATGTTAC
    TACCAGATTC AGCGTGAGAA GCTTAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89723
    Clone ID Related Accession (Same CDS sequence) XM_017004571.1 , XM_024453025.1
    Accession Version XM_017004571.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product alsin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGTATA TTGCCAGTCT CCACGAGTTA GCTACTACAG AAAGACGATT CTATTCAAAA 
    CTAAGTGATA TCAAATCTCA GATTCTCAGG CCTCTTCTCA GTTTAGAAAA TTTGGGCACT
    ACAACTACAG TCCAGCTGTT GCAGGAGGTG GCTAGCCGAT TCAGCAAGCT GTGTTACCTC
    ATTGGTCAGC ATGGAGCCTC ATTGAGCAGC TTCCTTCATG GGGTAAAGGA AGCCAGGAGT
    TTGGTCATCC TGAAGCATTC AAGTCTCTTC TTGGATAGTT ATACAGAGTA TTGCACATCT
    ATTACAAATT TCCTGGTTAT GGGAGGATTC CAGCTTCTTG CTAAGCCTGC CATTGATTTC
    CTAAATAAAA ACCAAGAGCT GTTGCAAGAT TTGTCAGAAG TGAATGACGA AAACACTCAG
    TTGATGGAAA TACTGAATAC TTTGTTTTTC TTGCCAATCA GACGACTTCA TAATTACGCA
    AAAGTTTTGC TAAAGCTTGC TACTTGTTTT GAAGTGGCAT CTCCAGAATA TCAGAAACTG
    CAGGATTCCA GTTCTTGTTA TGAGTGTCTT GCTCTCCATC TCGGCAGGAA AAGGAAGGAA
    GCAGAATACA CACTGGGCTT CTGGAAGACC TTCCCCGGAA AAATGACGGA TTCCTTGAGG
    AAGCCAGAGC GTCGACTGCT GTGTGAGAGT AGTAACCGAG CCCTGTCTCT GCAGCATGCT
    GGGAGGTTTT CCGTGAATTG GTTCATTCTC TTTAATGATG CCCTGGTCCA TGCCCAGTTC
    TCCACGCACC ATGTTTTCCC TCTGGCCACG CTGTGGGCAG AGCCACTGTC TGAAGAAGCT
    GGTGGTGTGA ATGGCTTAAA GATAACTACA CCTGAGGAGC AGTTCACTCT CATTTCATCT
    ACACCCCAGG AAAAGACAAA GTGGCTACGA GCTATAAGCC AAGCCGTAGA TCAGGCTTTG
    AGAGGGATGT CTGATCTCCC CCCTTATGGA AGTGGTAGCA GTGTTCAGAG ACAGGAACCA
    CCCATTTCAC GCAGTGCCAA ATATACTTTC TACAAGGATC CTCGCCTAAA GGATGCCACC
    TATGATGGAC GCTGGCTTTC AGGGAAGCCT CATGGCAGAG GGGTTTTGAA GTGGCCTGAT
    GGAAAGATGT ATTCTGGCAT GTTCAGGAAT GGCTTGGAAG ATGGGTATGG AGAATACAGA
    ATCCCAAACA AGGCAATGAA CAAAGAAGAC CATTATGTGG GCCATTGGAA AGAAGGAAAA
    ATGTGCGGTC AAGGAGTCTA CAGCTATGCT TCTGGTGAAG TATTTGAGGG CTGTTTTCAA
    GATAATATGC GTCATGGTCA TGGTCTTCTA CGAAGTGGGA AATTGACGTC CTCTTCTCCT
    AGTATGTTCA TTGGCCAGTG GGTAATGGAT AAGAAAGCAG GATATGGTGT CTTTGATGAT
    ATCACTAGGG GGGAAAAGTA TATGGGAATG TGGCAAGATG ATGTGTGTCA AGGGAATGGT
    GTGGTGGTTA CCCAGTTTGG ATTATACTAC GAGGGCAACT TTCACCTTAA TAAAATGATG
    GGAAATGGGG TTTTGCTTTC CGAAGATGAT ACTATCTATG AAGGAGAATT TTCAGATGAC
    TGGACTCTTA GTGGAAAGGG AACACTGACT ATGCCAAATG GAGACTACAT TGAAGGTTAT
    TTTAGTGGAG AATGGGGATC TGGGATAAAA ATCACTGGAA CCTACTTCAA ACCTAGTCTA
    TATGAGAGTG ATAAAGACAG ACCTAAAGTT TTGAAGCTAG GAAACCTGGC AGTGCCAGCT
    GATGAGAAGT GGAAAGCGGT GTTTGACGAA TGTTGGCGCC AACTGGGCTG TGAGGGCCCA
    GGCCAAGGGG AAGTTTGGAA AGCATGGGAC AATATTGCTG TGGCCTTGAC CACCAGTCGG
    CGCCAGCACA GAGACAGTCC AGAAATACTG AGTCGTTCAC AGACTCAGAC ACTAGAGAGT
    TTGGAATTCA TTCCACAGCA TGTTGGTGCC TTCTCTGTGG AGAAATATGA TGACATCAGG
    AAATATTTAA TAAAGGCCTG TGACACTCCT CTGCACCCCC TGGGCAGGCT TGTGGAGACA
    CTGGTTGCAG TGTATAGAAT GACATACGTG GGCGTAGGAG CCAACCGCAG GTTATTGCAG
    GAGGCTGTAA AGGAGATTAA GTCCTATCTT AAGCGAATTT TCCAGCTGGT GAGGTTCTTA
    TTTCCTGAGC TGCCTGAAGA AGGCAGCACA ATTCCTCTCT CTGCTCCTCT GCCAACCGAA
    AGGAAGTCTT TTTGCACTGG GAAGTCAGAT TCCCGATCTG AATCACCAGA GCCAGGTTAT
    GTAGTAACGA GTTCTGGATT ATTGCTTCCT GTGCTGCTAC CTCGGCTCTA CCCACCGCTG
    TTTATGCTTT ATGCTTTGGA TAATGATCGC GAGGAAGACA TTTACTGGGA ATGTGTCCTT
    CGACTAAATA AGCAGCCAGA TATTGCTCTC CTGGGCTTTC TTGGGGTGCA GAGGAAATTT
    TGGCCAGCAA CCTTGTCAAT CCTTGGAGAG AGTAAAAAGG TTTTGCCAAC CACGAAAGAT
    GCTTGTTTTG CCTCAGCAGT AGAATGTCTG CAGCAGATCA GCACAACATT TACCCCATCA
    GACAAACTTA AGGTCATCCA GCAGACTTTT GAGGAGATCT CTCAGAGTGT CCTGGCGTCA
    CTCCACGAAG ACTTCTTGTG GTCCATGGAT GACTTGTTTC CTGTTTTCTT ATATGTGGTG
    CTACGGGCCA GGATTAGGAA TTTAGGCTCT GAGGTACACC TCATTGAGGA TCTAATGGAC
    CCCTATCTTC AGCATGGGGA ACAGGGTATA ATGTTCACCA CCTTGAAGGC ATGTTACTAC
    CAGATTCAGC GTGAGAAGCT TAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860060.1
    CDS2063..4969
    Translation

    Target ORF information:

    RefSeq Version XM_017004571.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004571.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGTATA TTGCCAGTCT CCACGAGTTA GCTACTACAG AAAGACGATT CTATTCAAAA 
    CTAAGTGATA TCAAATCTCA GATTCTCAGG CCTCTTCTCA GTTTAGAAAA TTTGGGCACT
    ACAACTACAG TCCAGCTGTT GCAGGAGGTG GCTAGCCGAT TCAGCAAGCT GTGTTACCTC
    ATTGGTCAGC ATGGAGCCTC ATTGAGCAGC TTCCTTCATG GGGTAAAGGA AGCCAGGAGT
    TTGGTCATCC TGAAGCATTC AAGTCTCTTC TTGGATAGTT ATACAGAGTA TTGCACATCT
    ATTACAAATT TCCTGGTTAT GGGAGGATTC CAGCTTCTTG CTAAGCCTGC CATTGATTTC
    CTAAATAAAA ACCAAGAGCT GTTGCAAGAT TTGTCAGAAG TGAATGACGA AAACACTCAG
    TTGATGGAAA TACTGAATAC TTTGTTTTTC TTGCCAATCA GACGACTTCA TAATTACGCA
    AAAGTTTTGC TAAAGCTTGC TACTTGTTTT GAAGTGGCAT CTCCAGAATA TCAGAAACTG
    CAGGATTCCA GTTCTTGTTA TGAGTGTCTT GCTCTCCATC TCGGCAGGAA AAGGAAGGAA
    GCAGAATACA CACTGGGCTT CTGGAAGACC TTCCCCGGAA AAATGACGGA TTCCTTGAGG
    AAGCCAGAGC GTCGACTGCT GTGTGAGAGT AGTAACCGAG CCCTGTCTCT GCAGCATGCT
    GGGAGGTTTT CCGTGAATTG GTTCATTCTC TTTAATGATG CCCTGGTCCA TGCCCAGTTC
    TCCACGCACC ATGTTTTCCC TCTGGCCACG CTGTGGGCAG AGCCACTGTC TGAAGAAGCT
    GGTGGTGTGA ATGGCTTAAA GATAACTACA CCTGAGGAGC AGTTCACTCT CATTTCATCT
    ACACCCCAGG AAAAGACAAA GTGGCTACGA GCTATAAGCC AAGCCGTAGA TCAGGCTTTG
    AGAGGGATGT CTGATCTCCC CCCTTATGGA AGTGGTAGCA GTGTTCAGAG ACAGGAACCA
    CCCATTTCAC GCAGTGCCAA ATATACTTTC TACAAGGATC CTCGCCTAAA GGATGCCACC
    TATGATGGAC GCTGGCTTTC AGGGAAGCCT CATGGCAGAG GGGTTTTGAA GTGGCCTGAT
    GGAAAGATGT ATTCTGGCAT GTTCAGGAAT GGCTTGGAAG ATGGGTATGG AGAATACAGA
    ATCCCAAACA AGGCAATGAA CAAAGAAGAC CATTATGTGG GCCATTGGAA AGAAGGAAAA
    ATGTGCGGTC AAGGAGTCTA CAGCTATGCT TCTGGTGAAG TATTTGAGGG CTGTTTTCAA
    GATAATATGC GTCATGGTCA TGGTCTTCTA CGAAGTGGGA AATTGACGTC CTCTTCTCCT
    AGTATGTTCA TTGGCCAGTG GGTAATGGAT AAGAAAGCAG GATATGGTGT CTTTGATGAT
    ATCACTAGGG GGGAAAAGTA TATGGGAATG TGGCAAGATG ATGTGTGTCA AGGGAATGGT
    GTGGTGGTTA CCCAGTTTGG ATTATACTAC GAGGGCAACT TTCACCTTAA TAAAATGATG
    GGAAATGGGG TTTTGCTTTC CGAAGATGAT ACTATCTATG AAGGAGAATT TTCAGATGAC
    TGGACTCTTA GTGGAAAGGG AACACTGACT ATGCCAAATG GAGACTACAT TGAAGGTTAT
    TTTAGTGGAG AATGGGGATC TGGGATAAAA ATCACTGGAA CCTACTTCAA ACCTAGTCTA
    TATGAGAGTG ATAAAGACAG ACCTAAAGTT TTGAAGCTAG GAAACCTGGC AGTGCCAGCT
    GATGAGAAGT GGAAAGCGGT GTTTGACGAA TGTTGGCGCC AACTGGGCTG TGAGGGCCCA
    GGCCAAGGGG AAGTTTGGAA AGCATGGGAC AATATTGCTG TGGCCTTGAC CACCAGTCGG
    CGCCAGCACA GAGACAGTCC AGAAATACTG AGTCGTTCAC AGACTCAGAC ACTAGAGAGT
    TTGGAATTCA TTCCACAGCA TGTTGGTGCC TTCTCTGTGG AGAAATATGA TGACATCAGG
    AAATATTTAA TAAAGGCCTG TGACACTCCT CTGCACCCCC TGGGCAGGCT TGTGGAGACA
    CTGGTTGCAG TGTATAGAAT GACATACGTG GGCGTAGGAG CCAACCGCAG GTTATTGCAG
    GAGGCTGTAA AGGAGATTAA GTCCTATCTT AAGCGAATTT TCCAGCTGGT GAGGTTCTTA
    TTTCCTGAGC TGCCTGAAGA AGGCAGCACA ATTCCTCTCT CTGCTCCTCT GCCAACCGAA
    AGGAAGTCTT TTTGCACTGG GAAGTCAGAT TCCCGATCTG AATCACCAGA GCCAGGTTAT
    GTAGTAACGA GTTCTGGATT ATTGCTTCCT GTGCTGCTAC CTCGGCTCTA CCCACCGCTG
    TTTATGCTTT ATGCTTTGGA TAATGATCGC GAGGAAGACA TTTACTGGGA ATGTGTCCTT
    CGACTAAATA AGCAGCCAGA TATTGCTCTC CTGGGCTTTC TTGGGGTGCA GAGGAAATTT
    TGGCCAGCAA CCTTGTCAAT CCTTGGAGAG AGTAAAAAGG TTTTGCCAAC CACGAAAGAT
    GCTTGTTTTG CCTCAGCAGT AGAATGTCTG CAGCAGATCA GCACAACATT TACCCCATCA
    GACAAACTTA AGGTCATCCA GCAGACTTTT GAGGAGATCT CTCAGAGTGT CCTGGCGTCA
    CTCCACGAAG ACTTCTTGTG GTCCATGGAT GACTTGTTTC CTGTTTTCTT ATATGTGGTG
    CTACGGGCCA GGATTAGGAA TTTAGGCTCT GAGGTACACC TCATTGAGGA TCTAATGGAC
    CCCTATCTTC AGCATGGGGA ACAGGGTATA ATGTTCACCA CCTTGAAGGC ATGTTACTAC
    CAGATTCAGC GTGAGAAGCT TAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu89723
    Clone ID Related Accession (Same CDS sequence) XM_017004571.1 , XM_024453025.1
    Accession Version XM_024453025.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2907bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alsin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGTATA TTGCCAGTCT CCACGAGTTA GCTACTACAG AAAGACGATT CTATTCAAAA 
    CTAAGTGATA TCAAATCTCA GATTCTCAGG CCTCTTCTCA GTTTAGAAAA TTTGGGCACT
    ACAACTACAG TCCAGCTGTT GCAGGAGGTG GCTAGCCGAT TCAGCAAGCT GTGTTACCTC
    ATTGGTCAGC ATGGAGCCTC ATTGAGCAGC TTCCTTCATG GGGTAAAGGA AGCCAGGAGT
    TTGGTCATCC TGAAGCATTC AAGTCTCTTC TTGGATAGTT ATACAGAGTA TTGCACATCT
    ATTACAAATT TCCTGGTTAT GGGAGGATTC CAGCTTCTTG CTAAGCCTGC CATTGATTTC
    CTAAATAAAA ACCAAGAGCT GTTGCAAGAT TTGTCAGAAG TGAATGACGA AAACACTCAG
    TTGATGGAAA TACTGAATAC TTTGTTTTTC TTGCCAATCA GACGACTTCA TAATTACGCA
    AAAGTTTTGC TAAAGCTTGC TACTTGTTTT GAAGTGGCAT CTCCAGAATA TCAGAAACTG
    CAGGATTCCA GTTCTTGTTA TGAGTGTCTT GCTCTCCATC TCGGCAGGAA AAGGAAGGAA
    GCAGAATACA CACTGGGCTT CTGGAAGACC TTCCCCGGAA AAATGACGGA TTCCTTGAGG
    AAGCCAGAGC GTCGACTGCT GTGTGAGAGT AGTAACCGAG CCCTGTCTCT GCAGCATGCT
    GGGAGGTTTT CCGTGAATTG GTTCATTCTC TTTAATGATG CCCTGGTCCA TGCCCAGTTC
    TCCACGCACC ATGTTTTCCC TCTGGCCACG CTGTGGGCAG AGCCACTGTC TGAAGAAGCT
    GGTGGTGTGA ATGGCTTAAA GATAACTACA CCTGAGGAGC AGTTCACTCT CATTTCATCT
    ACACCCCAGG AAAAGACAAA GTGGCTACGA GCTATAAGCC AAGCCGTAGA TCAGGCTTTG
    AGAGGGATGT CTGATCTCCC CCCTTATGGA AGTGGTAGCA GTGTTCAGAG ACAGGAACCA
    CCCATTTCAC GCAGTGCCAA ATATACTTTC TACAAGGATC CTCGCCTAAA GGATGCCACC
    TATGATGGAC GCTGGCTTTC AGGGAAGCCT CATGGCAGAG GGGTTTTGAA GTGGCCTGAT
    GGAAAGATGT ATTCTGGCAT GTTCAGGAAT GGCTTGGAAG ATGGGTATGG AGAATACAGA
    ATCCCAAACA AGGCAATGAA CAAAGAAGAC CATTATGTGG GCCATTGGAA AGAAGGAAAA
    ATGTGCGGTC AAGGAGTCTA CAGCTATGCT TCTGGTGAAG TATTTGAGGG CTGTTTTCAA
    GATAATATGC GTCATGGTCA TGGTCTTCTA CGAAGTGGGA AATTGACGTC CTCTTCTCCT
    AGTATGTTCA TTGGCCAGTG GGTAATGGAT AAGAAAGCAG GATATGGTGT CTTTGATGAT
    ATCACTAGGG GGGAAAAGTA TATGGGAATG TGGCAAGATG ATGTGTGTCA AGGGAATGGT
    GTGGTGGTTA CCCAGTTTGG ATTATACTAC GAGGGCAACT TTCACCTTAA TAAAATGATG
    GGAAATGGGG TTTTGCTTTC CGAAGATGAT ACTATCTATG AAGGAGAATT TTCAGATGAC
    TGGACTCTTA GTGGAAAGGG AACACTGACT ATGCCAAATG GAGACTACAT TGAAGGTTAT
    TTTAGTGGAG AATGGGGATC TGGGATAAAA ATCACTGGAA CCTACTTCAA ACCTAGTCTA
    TATGAGAGTG ATAAAGACAG ACCTAAAGTT TTGAAGCTAG GAAACCTGGC AGTGCCAGCT
    GATGAGAAGT GGAAAGCGGT GTTTGACGAA TGTTGGCGCC AACTGGGCTG TGAGGGCCCA
    GGCCAAGGGG AAGTTTGGAA AGCATGGGAC AATATTGCTG TGGCCTTGAC CACCAGTCGG
    CGCCAGCACA GAGACAGTCC AGAAATACTG AGTCGTTCAC AGACTCAGAC ACTAGAGAGT
    TTGGAATTCA TTCCACAGCA TGTTGGTGCC TTCTCTGTGG AGAAATATGA TGACATCAGG
    AAATATTTAA TAAAGGCCTG TGACACTCCT CTGCACCCCC TGGGCAGGCT TGTGGAGACA
    CTGGTTGCAG TGTATAGAAT GACATACGTG GGCGTAGGAG CCAACCGCAG GTTATTGCAG
    GAGGCTGTAA AGGAGATTAA GTCCTATCTT AAGCGAATTT TCCAGCTGGT GAGGTTCTTA
    TTTCCTGAGC TGCCTGAAGA AGGCAGCACA ATTCCTCTCT CTGCTCCTCT GCCAACCGAA
    AGGAAGTCTT TTTGCACTGG GAAGTCAGAT TCCCGATCTG AATCACCAGA GCCAGGTTAT
    GTAGTAACGA GTTCTGGATT ATTGCTTCCT GTGCTGCTAC CTCGGCTCTA CCCACCGCTG
    TTTATGCTTT ATGCTTTGGA TAATGATCGC GAGGAAGACA TTTACTGGGA ATGTGTCCTT
    CGACTAAATA AGCAGCCAGA TATTGCTCTC CTGGGCTTTC TTGGGGTGCA GAGGAAATTT
    TGGCCAGCAA CCTTGTCAAT CCTTGGAGAG AGTAAAAAGG TTTTGCCAAC CACGAAAGAT
    GCTTGTTTTG CCTCAGCAGT AGAATGTCTG CAGCAGATCA GCACAACATT TACCCCATCA
    GACAAACTTA AGGTCATCCA GCAGACTTTT GAGGAGATCT CTCAGAGTGT CCTGGCGTCA
    CTCCACGAAG ACTTCTTGTG GTCCATGGAT GACTTGTTTC CTGTTTTCTT ATATGTGGTG
    CTACGGGCCA GGATTAGGAA TTTAGGCTCT GAGGTACACC TCATTGAGGA TCTAATGGAC
    CCCTATCTTC AGCATGGGGA ACAGGGTATA ATGTTCACCA CCTTGAAGGC ATGTTACTAC
    CAGATTCAGC GTGAGAAGCT TAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308793.1
    CDS1256..4162
    Translation

    Target ORF information:

    RefSeq Version XM_024453025.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453025.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    ATGGGGTATA TTGCCAGTCT CCACGAGTTA GCTACTACAG AAAGACGATT CTATTCAAAA 
    CTAAGTGATA TCAAATCTCA GATTCTCAGG CCTCTTCTCA GTTTAGAAAA TTTGGGCACT
    ACAACTACAG TCCAGCTGTT GCAGGAGGTG GCTAGCCGAT TCAGCAAGCT GTGTTACCTC
    ATTGGTCAGC ATGGAGCCTC ATTGAGCAGC TTCCTTCATG GGGTAAAGGA AGCCAGGAGT
    TTGGTCATCC TGAAGCATTC AAGTCTCTTC TTGGATAGTT ATACAGAGTA TTGCACATCT
    ATTACAAATT TCCTGGTTAT GGGAGGATTC CAGCTTCTTG CTAAGCCTGC CATTGATTTC
    CTAAATAAAA ACCAAGAGCT GTTGCAAGAT TTGTCAGAAG TGAATGACGA AAACACTCAG
    TTGATGGAAA TACTGAATAC TTTGTTTTTC TTGCCAATCA GACGACTTCA TAATTACGCA
    AAAGTTTTGC TAAAGCTTGC TACTTGTTTT GAAGTGGCAT CTCCAGAATA TCAGAAACTG
    CAGGATTCCA GTTCTTGTTA TGAGTGTCTT GCTCTCCATC TCGGCAGGAA AAGGAAGGAA
    GCAGAATACA CACTGGGCTT CTGGAAGACC TTCCCCGGAA AAATGACGGA TTCCTTGAGG
    AAGCCAGAGC GTCGACTGCT GTGTGAGAGT AGTAACCGAG CCCTGTCTCT GCAGCATGCT
    GGGAGGTTTT CCGTGAATTG GTTCATTCTC TTTAATGATG CCCTGGTCCA TGCCCAGTTC
    TCCACGCACC ATGTTTTCCC TCTGGCCACG CTGTGGGCAG AGCCACTGTC TGAAGAAGCT
    GGTGGTGTGA ATGGCTTAAA GATAACTACA CCTGAGGAGC AGTTCACTCT CATTTCATCT
    ACACCCCAGG AAAAGACAAA GTGGCTACGA GCTATAAGCC AAGCCGTAGA TCAGGCTTTG
    AGAGGGATGT CTGATCTCCC CCCTTATGGA AGTGGTAGCA GTGTTCAGAG ACAGGAACCA
    CCCATTTCAC GCAGTGCCAA ATATACTTTC TACAAGGATC CTCGCCTAAA GGATGCCACC
    TATGATGGAC GCTGGCTTTC AGGGAAGCCT CATGGCAGAG GGGTTTTGAA GTGGCCTGAT
    GGAAAGATGT ATTCTGGCAT GTTCAGGAAT GGCTTGGAAG ATGGGTATGG AGAATACAGA
    ATCCCAAACA AGGCAATGAA CAAAGAAGAC CATTATGTGG GCCATTGGAA AGAAGGAAAA
    ATGTGCGGTC AAGGAGTCTA CAGCTATGCT TCTGGTGAAG TATTTGAGGG CTGTTTTCAA
    GATAATATGC GTCATGGTCA TGGTCTTCTA CGAAGTGGGA AATTGACGTC CTCTTCTCCT
    AGTATGTTCA TTGGCCAGTG GGTAATGGAT AAGAAAGCAG GATATGGTGT CTTTGATGAT
    ATCACTAGGG GGGAAAAGTA TATGGGAATG TGGCAAGATG ATGTGTGTCA AGGGAATGGT
    GTGGTGGTTA CCCAGTTTGG ATTATACTAC GAGGGCAACT TTCACCTTAA TAAAATGATG
    GGAAATGGGG TTTTGCTTTC CGAAGATGAT ACTATCTATG AAGGAGAATT TTCAGATGAC
    TGGACTCTTA GTGGAAAGGG AACACTGACT ATGCCAAATG GAGACTACAT TGAAGGTTAT
    TTTAGTGGAG AATGGGGATC TGGGATAAAA ATCACTGGAA CCTACTTCAA ACCTAGTCTA
    TATGAGAGTG ATAAAGACAG ACCTAAAGTT TTGAAGCTAG GAAACCTGGC AGTGCCAGCT
    GATGAGAAGT GGAAAGCGGT GTTTGACGAA TGTTGGCGCC AACTGGGCTG TGAGGGCCCA
    GGCCAAGGGG AAGTTTGGAA AGCATGGGAC AATATTGCTG TGGCCTTGAC CACCAGTCGG
    CGCCAGCACA GAGACAGTCC AGAAATACTG AGTCGTTCAC AGACTCAGAC ACTAGAGAGT
    TTGGAATTCA TTCCACAGCA TGTTGGTGCC TTCTCTGTGG AGAAATATGA TGACATCAGG
    AAATATTTAA TAAAGGCCTG TGACACTCCT CTGCACCCCC TGGGCAGGCT TGTGGAGACA
    CTGGTTGCAG TGTATAGAAT GACATACGTG GGCGTAGGAG CCAACCGCAG GTTATTGCAG
    GAGGCTGTAA AGGAGATTAA GTCCTATCTT AAGCGAATTT TCCAGCTGGT GAGGTTCTTA
    TTTCCTGAGC TGCCTGAAGA AGGCAGCACA ATTCCTCTCT CTGCTCCTCT GCCAACCGAA
    AGGAAGTCTT TTTGCACTGG GAAGTCAGAT TCCCGATCTG AATCACCAGA GCCAGGTTAT
    GTAGTAACGA GTTCTGGATT ATTGCTTCCT GTGCTGCTAC CTCGGCTCTA CCCACCGCTG
    TTTATGCTTT ATGCTTTGGA TAATGATCGC GAGGAAGACA TTTACTGGGA ATGTGTCCTT
    CGACTAAATA AGCAGCCAGA TATTGCTCTC CTGGGCTTTC TTGGGGTGCA GAGGAAATTT
    TGGCCAGCAA CCTTGTCAAT CCTTGGAGAG AGTAAAAAGG TTTTGCCAAC CACGAAAGAT
    GCTTGTTTTG CCTCAGCAGT AGAATGTCTG CAGCAGATCA GCACAACATT TACCCCATCA
    GACAAACTTA AGGTCATCCA GCAGACTTTT GAGGAGATCT CTCAGAGTGT CCTGGCGTCA
    CTCCACGAAG ACTTCTTGTG GTCCATGGAT GACTTGTTTC CTGTTTTCTT ATATGTGGTG
    CTACGGGCCA GGATTAGGAA TTTAGGCTCT GAGGTACACC TCATTGAGGA TCTAATGGAC
    CCCTATCTTC AGCATGGGGA ACAGGGTATA ATGTTCACCA CCTTGAAGGC ATGTTACTAC
    CAGATTCAGC GTGAGAAGCT TAACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19615
    Clone ID Related Accession (Same CDS sequence) NM_020919.3 , XM_006712654.1 , XM_006712654.2 , NM_020919.4 , XM_006712654.3
    Accession Version NM_020919.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-12-26
    Organism Homo sapiens(human)
    Product alsin isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP348546.1, AC007242.3, AK291029.1, AF391100.1 and BM978733.1. This sequence is a reference standard in the RefSeqGene project. On Oct 10, 2008 this sequence version replaced NM_020919.2. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1), also known as the LF or long form. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.19136.1, SRR1660807.141972.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGTCCAGA AATACTGAGT
    CGTTCACAGA CTCAGACACT AGAGAGTTTG GAATTCATTC CACAGCATGT TGGTGCCTTC
    TCTGTGGAGA AATATGATGA CATCAGGAAA TATTTAATAA AGGCCTGTGA CACTCCTCTG
    CACCCCCTGG GCAGGCTTGT GGAGACACTG GTTGCAGTGT ATAGAATGAC ATACGTGGGC
    GTAGGAGCCA ACCGCAGGTT ATTGCAGGAG GCTGTAAAGG AGATTAAGTC CTATCTTAAG
    CGAATTTTCC AGCTGGTGAG GTTCTTATTT CCTGAGCTGC CTGAAGAAGG CAGCACAATT
    CCTCTCTCTG CTCCTCTGCC AACCGAAAGG AAGTCTTTTT GCACTGGGAA GTCAGATTCC
    CGATCTGAAT CACCAGAGCC AGGTTATGTA GTAACGAGTT CTGGATTATT GCTTCCTGTG
    CTGCTACCTC GGCTCTACCC ACCGCTGTTT ATGCTTTATG CTTTGGATAA TGATCGCGAG
    GAAGACATTT ACTGGGAATG TGTCCTTCGA CTAAATAAGC AGCCAGATAT TGCTCTCCTG
    GGCTTTCTTG GGGTGCAGAG GAAATTTTGG CCAGCAACCT TGTCAATCCT TGGAGAGAGT
    AAAAAGGTTT TGCCAACCAC GAAAGATGCT TGTTTTGCCT CAGCAGTAGA ATGTCTGCAG
    CAGATCAGCA CAACATTTAC CCCATCAGAC AAACTTAAGG TCATCCAGCA GACTTTTGAG
    GAGATCTCTC AGAGTGTCCT GGCGTCACTC CACGAAGACT TCTTGTGGTC CATGGATGAC
    TTGTTTCCTG TTTTCTTATA TGTGGTGCTA CGGGCCAGGA TTAGGAATTT AGGCTCTGAG
    GTACACCTCA TTGAGGATCT AATGGACCCC TATCTTCAGC ATGGGGAACA GGGTATAATG
    TTCACCACCT TGAAGGCATG TTACTACCAG ATTCAGCGTG AGAAGCTTAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065970.2
    CDS357..5330
    Translation

    Target ORF information:

    RefSeq Version NM_020919.3
    Organism Homo sapiens(human)
    Definition Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020919.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGTCCAGA AATACTGAGT
    CGTTCACAGA CTCAGACACT AGAGAGTTTG GAATTCATTC CACAGCATGT TGGTGCCTTC
    TCTGTGGAGA AATATGATGA CATCAGGAAA TATTTAATAA AGGCCTGTGA CACTCCTCTG
    CACCCCCTGG GCAGGCTTGT GGAGACACTG GTTGCAGTGT ATAGAATGAC ATACGTGGGC
    GTAGGAGCCA ACCGCAGGTT ATTGCAGGAG GCTGTAAAGG AGATTAAGTC CTATCTTAAG
    CGAATTTTCC AGCTGGTGAG GTTCTTATTT CCTGAGCTGC CTGAAGAAGG CAGCACAATT
    CCTCTCTCTG CTCCTCTGCC AACCGAAAGG AAGTCTTTTT GCACTGGGAA GTCAGATTCC
    CGATCTGAAT CACCAGAGCC AGGTTATGTA GTAACGAGTT CTGGATTATT GCTTCCTGTG
    CTGCTACCTC GGCTCTACCC ACCGCTGTTT ATGCTTTATG CTTTGGATAA TGATCGCGAG
    GAAGACATTT ACTGGGAATG TGTCCTTCGA CTAAATAAGC AGCCAGATAT TGCTCTCCTG
    GGCTTTCTTG GGGTGCAGAG GAAATTTTGG CCAGCAACCT TGTCAATCCT TGGAGAGAGT
    AAAAAGGTTT TGCCAACCAC GAAAGATGCT TGTTTTGCCT CAGCAGTAGA ATGTCTGCAG
    CAGATCAGCA CAACATTTAC CCCATCAGAC AAACTTAAGG TCATCCAGCA GACTTTTGAG
    GAGATCTCTC AGAGTGTCCT GGCGTCACTC CACGAAGACT TCTTGTGGTC CATGGATGAC
    TTGTTTCCTG TTTTCTTATA TGTGGTGCTA CGGGCCAGGA TTAGGAATTT AGGCTCTGAG
    GTACACCTCA TTGAGGATCT AATGGACCCC TATCTTCAGC ATGGGGAACA GGGTATAATG
    TTCACCACCT TGAAGGCATG TTACTACCAG ATTCAGCGTG AGAAGCTTAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19615
    Clone ID Related Accession (Same CDS sequence) NM_020919.3 , XM_006712654.1 , XM_006712654.2 , NM_020919.4 , XM_006712654.3
    Accession Version NM_020919.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-30
    Organism Homo sapiens(human)
    Product alsin isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK291029.1, AF391100.1 and BM978733.1. This sequence is a reference standard in the RefSeqGene project. On Nov 23, 2018 this sequence version replaced NM_020919.3. Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1), also known as the LF or long form. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB053305.1, AF391100.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000264276.11/ ENSP00000264276.6 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGTCCAGA AATACTGAGT
    CGTTCACAGA CTCAGACACT AGAGAGTTTG GAATTCATTC CACAGCATGT TGGTGCCTTC
    TCTGTGGAGA AATATGATGA CATCAGGAAA TATTTAATAA AGGCCTGTGA CACTCCTCTG
    CACCCCCTGG GCAGGCTTGT GGAGACACTG GTTGCAGTGT ATAGAATGAC ATACGTGGGC
    GTAGGAGCCA ACCGCAGGTT ATTGCAGGAG GCTGTAAAGG AGATTAAGTC CTATCTTAAG
    CGAATTTTCC AGCTGGTGAG GTTCTTATTT CCTGAGCTGC CTGAAGAAGG CAGCACAATT
    CCTCTCTCTG CTCCTCTGCC AACCGAAAGG AAGTCTTTTT GCACTGGGAA GTCAGATTCC
    CGATCTGAAT CACCAGAGCC AGGTTATGTA GTAACGAGTT CTGGATTATT GCTTCCTGTG
    CTGCTACCTC GGCTCTACCC ACCGCTGTTT ATGCTTTATG CTTTGGATAA TGATCGCGAG
    GAAGACATTT ACTGGGAATG TGTCCTTCGA CTAAATAAGC AGCCAGATAT TGCTCTCCTG
    GGCTTTCTTG GGGTGCAGAG GAAATTTTGG CCAGCAACCT TGTCAATCCT TGGAGAGAGT
    AAAAAGGTTT TGCCAACCAC GAAAGATGCT TGTTTTGCCT CAGCAGTAGA ATGTCTGCAG
    CAGATCAGCA CAACATTTAC CCCATCAGAC AAACTTAAGG TCATCCAGCA GACTTTTGAG
    GAGATCTCTC AGAGTGTCCT GGCGTCACTC CACGAAGACT TCTTGTGGTC CATGGATGAC
    TTGTTTCCTG TTTTCTTATA TGTGGTGCTA CGGGCCAGGA TTAGGAATTT AGGCTCTGAG
    GTACACCTCA TTGAGGATCT AATGGACCCC TATCTTCAGC ATGGGGAACA GGGTATAATG
    TTCACCACCT TGAAGGCATG TTACTACCAG ATTCAGCGTG AGAAGCTTAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065970.2
    CDS118..5091
    Translation

    Target ORF information:

    RefSeq Version NM_020919.4
    Organism Homo sapiens(human)
    Definition Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020919.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGTCCAGA AATACTGAGT
    CGTTCACAGA CTCAGACACT AGAGAGTTTG GAATTCATTC CACAGCATGT TGGTGCCTTC
    TCTGTGGAGA AATATGATGA CATCAGGAAA TATTTAATAA AGGCCTGTGA CACTCCTCTG
    CACCCCCTGG GCAGGCTTGT GGAGACACTG GTTGCAGTGT ATAGAATGAC ATACGTGGGC
    GTAGGAGCCA ACCGCAGGTT ATTGCAGGAG GCTGTAAAGG AGATTAAGTC CTATCTTAAG
    CGAATTTTCC AGCTGGTGAG GTTCTTATTT CCTGAGCTGC CTGAAGAAGG CAGCACAATT
    CCTCTCTCTG CTCCTCTGCC AACCGAAAGG AAGTCTTTTT GCACTGGGAA GTCAGATTCC
    CGATCTGAAT CACCAGAGCC AGGTTATGTA GTAACGAGTT CTGGATTATT GCTTCCTGTG
    CTGCTACCTC GGCTCTACCC ACCGCTGTTT ATGCTTTATG CTTTGGATAA TGATCGCGAG
    GAAGACATTT ACTGGGAATG TGTCCTTCGA CTAAATAAGC AGCCAGATAT TGCTCTCCTG
    GGCTTTCTTG GGGTGCAGAG GAAATTTTGG CCAGCAACCT TGTCAATCCT TGGAGAGAGT
    AAAAAGGTTT TGCCAACCAC GAAAGATGCT TGTTTTGCCT CAGCAGTAGA ATGTCTGCAG
    CAGATCAGCA CAACATTTAC CCCATCAGAC AAACTTAAGG TCATCCAGCA GACTTTTGAG
    GAGATCTCTC AGAGTGTCCT GGCGTCACTC CACGAAGACT TCTTGTGGTC CATGGATGAC
    TTGTTTCCTG TTTTCTTATA TGTGGTGCTA CGGGCCAGGA TTAGGAATTT AGGCTCTGAG
    GTACACCTCA TTGAGGATCT AATGGACCCC TATCTTCAGC ATGGGGAACA GGGTATAATG
    TTCACCACCT TGAAGGCATG TTACTACCAG ATTCAGCGTG AGAAGCTTAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19615
    Clone ID Related Accession (Same CDS sequence) NM_020919.3 , XM_006712654.1 , XM_006712654.2 , NM_020919.4 , XM_006712654.3
    Accession Version XM_006712654.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product alsin isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGTCCAGA AATACTGAGT
    CGTTCACAGA CTCAGACACT AGAGAGTTTG GAATTCATTC CACAGCATGT TGGTGCCTTC
    TCTGTGGAGA AATATGATGA CATCAGGAAA TATTTAATAA AGGCCTGTGA CACTCCTCTG
    CACCCCCTGG GCAGGCTTGT GGAGACACTG GTTGCAGTGT ATAGAATGAC ATACGTGGGC
    GTAGGAGCCA ACCGCAGGTT ATTGCAGGAG GCTGTAAAGG AGATTAAGTC CTATCTTAAG
    CGAATTTTCC AGCTGGTGAG GTTCTTATTT CCTGAGCTGC CTGAAGAAGG CAGCACAATT
    CCTCTCTCTG CTCCTCTGCC AACCGAAAGG AAGTCTTTTT GCACTGGGAA GTCAGATTCC
    CGATCTGAAT CACCAGAGCC AGGTTATGTA GTAACGAGTT CTGGATTATT GCTTCCTGTG
    CTGCTACCTC GGCTCTACCC ACCGCTGTTT ATGCTTTATG CTTTGGATAA TGATCGCGAG
    GAAGACATTT ACTGGGAATG TGTCCTTCGA CTAAATAAGC AGCCAGATAT TGCTCTCCTG
    GGCTTTCTTG GGGTGCAGAG GAAATTTTGG CCAGCAACCT TGTCAATCCT TGGAGAGAGT
    AAAAAGGTTT TGCCAACCAC GAAAGATGCT TGTTTTGCCT CAGCAGTAGA ATGTCTGCAG
    CAGATCAGCA CAACATTTAC CCCATCAGAC AAACTTAAGG TCATCCAGCA GACTTTTGAG
    GAGATCTCTC AGAGTGTCCT GGCGTCACTC CACGAAGACT TCTTGTGGTC CATGGATGAC
    TTGTTTCCTG TTTTCTTATA TGTGGTGCTA CGGGCCAGGA TTAGGAATTT AGGCTCTGAG
    GTACACCTCA TTGAGGATCT AATGGACCCC TATCTTCAGC ATGGGGAACA GGGTATAATG
    TTCACCACCT TGAAGGCATG TTACTACCAG ATTCAGCGTG AGAAGCTTAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712717.1
    Translation

    Target ORF information:

    RefSeq Version XM_006712654.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens amyotrophic lateral sclerosis 2 (juvenile) (ALS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGTCCAGA AATACTGAGT
    CGTTCACAGA CTCAGACACT AGAGAGTTTG GAATTCATTC CACAGCATGT TGGTGCCTTC
    TCTGTGGAGA AATATGATGA CATCAGGAAA TATTTAATAA AGGCCTGTGA CACTCCTCTG
    CACCCCCTGG GCAGGCTTGT GGAGACACTG GTTGCAGTGT ATAGAATGAC ATACGTGGGC
    GTAGGAGCCA ACCGCAGGTT ATTGCAGGAG GCTGTAAAGG AGATTAAGTC CTATCTTAAG
    CGAATTTTCC AGCTGGTGAG GTTCTTATTT CCTGAGCTGC CTGAAGAAGG CAGCACAATT
    CCTCTCTCTG CTCCTCTGCC AACCGAAAGG AAGTCTTTTT GCACTGGGAA GTCAGATTCC
    CGATCTGAAT CACCAGAGCC AGGTTATGTA GTAACGAGTT CTGGATTATT GCTTCCTGTG
    CTGCTACCTC GGCTCTACCC ACCGCTGTTT ATGCTTTATG CTTTGGATAA TGATCGCGAG
    GAAGACATTT ACTGGGAATG TGTCCTTCGA CTAAATAAGC AGCCAGATAT TGCTCTCCTG
    GGCTTTCTTG GGGTGCAGAG GAAATTTTGG CCAGCAACCT TGTCAATCCT TGGAGAGAGT
    AAAAAGGTTT TGCCAACCAC GAAAGATGCT TGTTTTGCCT CAGCAGTAGA ATGTCTGCAG
    CAGATCAGCA CAACATTTAC CCCATCAGAC AAACTTAAGG TCATCCAGCA GACTTTTGAG
    GAGATCTCTC AGAGTGTCCT GGCGTCACTC CACGAAGACT TCTTGTGGTC CATGGATGAC
    TTGTTTCCTG TTTTCTTATA TGTGGTGCTA CGGGCCAGGA TTAGGAATTT AGGCTCTGAG
    GTACACCTCA TTGAGGATCT AATGGACCCC TATCTTCAGC ATGGGGAACA GGGTATAATG
    TTCACCACCT TGAAGGCATG TTACTACCAG ATTCAGCGTG AGAAGCTTAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19615
    Clone ID Related Accession (Same CDS sequence) NM_020919.3 , XM_006712654.1 , XM_006712654.2 , NM_020919.4 , XM_006712654.3
    Accession Version XM_006712654.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_006712654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGTCCAGA AATACTGAGT
    CGTTCACAGA CTCAGACACT AGAGAGTTTG GAATTCATTC CACAGCATGT TGGTGCCTTC
    TCTGTGGAGA AATATGATGA CATCAGGAAA TATTTAATAA AGGCCTGTGA CACTCCTCTG
    CACCCCCTGG GCAGGCTTGT GGAGACACTG GTTGCAGTGT ATAGAATGAC ATACGTGGGC
    GTAGGAGCCA ACCGCAGGTT ATTGCAGGAG GCTGTAAAGG AGATTAAGTC CTATCTTAAG
    CGAATTTTCC AGCTGGTGAG GTTCTTATTT CCTGAGCTGC CTGAAGAAGG CAGCACAATT
    CCTCTCTCTG CTCCTCTGCC AACCGAAAGG AAGTCTTTTT GCACTGGGAA GTCAGATTCC
    CGATCTGAAT CACCAGAGCC AGGTTATGTA GTAACGAGTT CTGGATTATT GCTTCCTGTG
    CTGCTACCTC GGCTCTACCC ACCGCTGTTT ATGCTTTATG CTTTGGATAA TGATCGCGAG
    GAAGACATTT ACTGGGAATG TGTCCTTCGA CTAAATAAGC AGCCAGATAT TGCTCTCCTG
    GGCTTTCTTG GGGTGCAGAG GAAATTTTGG CCAGCAACCT TGTCAATCCT TGGAGAGAGT
    AAAAAGGTTT TGCCAACCAC GAAAGATGCT TGTTTTGCCT CAGCAGTAGA ATGTCTGCAG
    CAGATCAGCA CAACATTTAC CCCATCAGAC AAACTTAAGG TCATCCAGCA GACTTTTGAG
    GAGATCTCTC AGAGTGTCCT GGCGTCACTC CACGAAGACT TCTTGTGGTC CATGGATGAC
    TTGTTTCCTG TTTTCTTATA TGTGGTGCTA CGGGCCAGGA TTAGGAATTT AGGCTCTGAG
    GTACACCTCA TTGAGGATCT AATGGACCCC TATCTTCAGC ATGGGGAACA GGGTATAATG
    TTCACCACCT TGAAGGCATG TTACTACCAG ATTCAGCGTG AGAAGCTTAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712717.1
    CDS934..5907
    Translation

    Target ORF information:

    RefSeq Version XM_006712654.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712654.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGTCCAGA AATACTGAGT
    CGTTCACAGA CTCAGACACT AGAGAGTTTG GAATTCATTC CACAGCATGT TGGTGCCTTC
    TCTGTGGAGA AATATGATGA CATCAGGAAA TATTTAATAA AGGCCTGTGA CACTCCTCTG
    CACCCCCTGG GCAGGCTTGT GGAGACACTG GTTGCAGTGT ATAGAATGAC ATACGTGGGC
    GTAGGAGCCA ACCGCAGGTT ATTGCAGGAG GCTGTAAAGG AGATTAAGTC CTATCTTAAG
    CGAATTTTCC AGCTGGTGAG GTTCTTATTT CCTGAGCTGC CTGAAGAAGG CAGCACAATT
    CCTCTCTCTG CTCCTCTGCC AACCGAAAGG AAGTCTTTTT GCACTGGGAA GTCAGATTCC
    CGATCTGAAT CACCAGAGCC AGGTTATGTA GTAACGAGTT CTGGATTATT GCTTCCTGTG
    CTGCTACCTC GGCTCTACCC ACCGCTGTTT ATGCTTTATG CTTTGGATAA TGATCGCGAG
    GAAGACATTT ACTGGGAATG TGTCCTTCGA CTAAATAAGC AGCCAGATAT TGCTCTCCTG
    GGCTTTCTTG GGGTGCAGAG GAAATTTTGG CCAGCAACCT TGTCAATCCT TGGAGAGAGT
    AAAAAGGTTT TGCCAACCAC GAAAGATGCT TGTTTTGCCT CAGCAGTAGA ATGTCTGCAG
    CAGATCAGCA CAACATTTAC CCCATCAGAC AAACTTAAGG TCATCCAGCA GACTTTTGAG
    GAGATCTCTC AGAGTGTCCT GGCGTCACTC CACGAAGACT TCTTGTGGTC CATGGATGAC
    TTGTTTCCTG TTTTCTTATA TGTGGTGCTA CGGGCCAGGA TTAGGAATTT AGGCTCTGAG
    GTACACCTCA TTGAGGATCT AATGGACCCC TATCTTCAGC ATGGGGAACA GGGTATAATG
    TTCACCACCT TGAAGGCATG TTACTACCAG ATTCAGCGTG AGAAGCTTAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu19615
    Clone ID Related Accession (Same CDS sequence) NM_020919.3 , XM_006712654.1 , XM_006712654.2 , NM_020919.4 , XM_006712654.3
    Accession Version XM_006712654.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4974bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alsin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_006712654.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGTCCAGA AATACTGAGT
    CGTTCACAGA CTCAGACACT AGAGAGTTTG GAATTCATTC CACAGCATGT TGGTGCCTTC
    TCTGTGGAGA AATATGATGA CATCAGGAAA TATTTAATAA AGGCCTGTGA CACTCCTCTG
    CACCCCCTGG GCAGGCTTGT GGAGACACTG GTTGCAGTGT ATAGAATGAC ATACGTGGGC
    GTAGGAGCCA ACCGCAGGTT ATTGCAGGAG GCTGTAAAGG AGATTAAGTC CTATCTTAAG
    CGAATTTTCC AGCTGGTGAG GTTCTTATTT CCTGAGCTGC CTGAAGAAGG CAGCACAATT
    CCTCTCTCTG CTCCTCTGCC AACCGAAAGG AAGTCTTTTT GCACTGGGAA GTCAGATTCC
    CGATCTGAAT CACCAGAGCC AGGTTATGTA GTAACGAGTT CTGGATTATT GCTTCCTGTG
    CTGCTACCTC GGCTCTACCC ACCGCTGTTT ATGCTTTATG CTTTGGATAA TGATCGCGAG
    GAAGACATTT ACTGGGAATG TGTCCTTCGA CTAAATAAGC AGCCAGATAT TGCTCTCCTG
    GGCTTTCTTG GGGTGCAGAG GAAATTTTGG CCAGCAACCT TGTCAATCCT TGGAGAGAGT
    AAAAAGGTTT TGCCAACCAC GAAAGATGCT TGTTTTGCCT CAGCAGTAGA ATGTCTGCAG
    CAGATCAGCA CAACATTTAC CCCATCAGAC AAACTTAAGG TCATCCAGCA GACTTTTGAG
    GAGATCTCTC AGAGTGTCCT GGCGTCACTC CACGAAGACT TCTTGTGGTC CATGGATGAC
    TTGTTTCCTG TTTTCTTATA TGTGGTGCTA CGGGCCAGGA TTAGGAATTT AGGCTCTGAG
    GTACACCTCA TTGAGGATCT AATGGACCCC TATCTTCAGC ATGGGGAACA GGGTATAATG
    TTCACCACCT TGAAGGCATG TTACTACCAG ATTCAGCGTG AGAAGCTTAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006712717.1
    CDS961..5934
    Translation

    Target ORF information:

    RefSeq Version XM_006712654.3
    Organism Homo sapiens(human)
    Definition Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006712654.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGTCCAGA AATACTGAGT
    CGTTCACAGA CTCAGACACT AGAGAGTTTG GAATTCATTC CACAGCATGT TGGTGCCTTC
    TCTGTGGAGA AATATGATGA CATCAGGAAA TATTTAATAA AGGCCTGTGA CACTCCTCTG
    CACCCCCTGG GCAGGCTTGT GGAGACACTG GTTGCAGTGT ATAGAATGAC ATACGTGGGC
    GTAGGAGCCA ACCGCAGGTT ATTGCAGGAG GCTGTAAAGG AGATTAAGTC CTATCTTAAG
    CGAATTTTCC AGCTGGTGAG GTTCTTATTT CCTGAGCTGC CTGAAGAAGG CAGCACAATT
    CCTCTCTCTG CTCCTCTGCC AACCGAAAGG AAGTCTTTTT GCACTGGGAA GTCAGATTCC
    CGATCTGAAT CACCAGAGCC AGGTTATGTA GTAACGAGTT CTGGATTATT GCTTCCTGTG
    CTGCTACCTC GGCTCTACCC ACCGCTGTTT ATGCTTTATG CTTTGGATAA TGATCGCGAG
    GAAGACATTT ACTGGGAATG TGTCCTTCGA CTAAATAAGC AGCCAGATAT TGCTCTCCTG
    GGCTTTCTTG GGGTGCAGAG GAAATTTTGG CCAGCAACCT TGTCAATCCT TGGAGAGAGT
    AAAAAGGTTT TGCCAACCAC GAAAGATGCT TGTTTTGCCT CAGCAGTAGA ATGTCTGCAG
    CAGATCAGCA CAACATTTAC CCCATCAGAC AAACTTAAGG TCATCCAGCA GACTTTTGAG
    GAGATCTCTC AGAGTGTCCT GGCGTCACTC CACGAAGACT TCTTGTGGTC CATGGATGAC
    TTGTTTCCTG TTTTCTTATA TGTGGTGCTA CGGGCCAGGA TTAGGAATTT AGGCTCTGAG
    GTACACCTCA TTGAGGATCT AATGGACCCC TATCTTCAGC ATGGGGAACA GGGTATAATG
    TTCACCACCT TGAAGGCATG TTACTACCAG ATTCAGCGTG AGAAGCTTAA CTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36475
    Clone ID Related Accession (Same CDS sequence) XM_017004569.1 , XM_017004569.2
    Accession Version XM_017004569.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product alsin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTGAAG
    CTAGGAAACC TGGCAGTGCC AGCTGATGAG AAGTGGAAAG CGGTGTTTGA CGAATGTTGG
    CGCCAACTGG GCTGTGAGGG CCCAGGCCAA GGGGAAGTTT GGAAAGCATG GGACAATATT
    GCTGTGGCCT TGACCACCAG TCGGCGCCAG CACAGAGACA GTCCAGAAAT ACTGAGTCGT
    TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCAC AGCATGTTGG TGCCTTCTCT
    GTGGAGAAAT ATGATGACAT CAGGAAATAT TTAATAAAGG CCTGTGACAC TCCTCTGCAC
    CCCCTGGGCA GGCTTGTGGA GACACTGGTT GCAGTGTATA GAATGACATA CGTGGGCGTA
    GGAGCCAACC GCAGGTTATT GCAGGAGGCT GTAAAGGAGA TTAAGTCCTA TCTTAAGCGA
    ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTGCCTG AAGAAGGCAG CACAATTCCT
    CTCTCTGCTC CTCTGCCAAC CGAAAGGAAG TCTTTTTGCA CTGGGAAGTC AGATTCCCGA
    TCTGAATCAC CAGAGCCAGG TTATGTAGTA ACGAGTTCTG GATTATTGCT TCCTGTGCTG
    CTACCTCGGC TCTACCCACC GCTGTTTATG CTTTATGCTT TGGATAATGA TCGCGAGGAA
    GACATTTACT GGGAATGTGT CCTTCGACTA AATAAGCAGC CAGATATTGC TCTCCTGGGC
    TTTCTTGGGG TGCAGAGGAA ATTTTGGCCA GCAACCTTGT CAATCCTTGG AGAGAGTAAA
    AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CAGTAGAATG TCTGCAGCAG
    ATCAGCACAA CATTTACCCC ATCAGACAAA CTTAAGGTCA TCCAGCAGAC TTTTGAGGAG
    ATCTCTCAGA GTGTCCTGGC GTCACTCCAC GAAGACTTCT TGTGGTCCAT GGATGACTTG
    TTTCCTGTTT TCTTATATGT GGTGCTACGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA
    CACCTCATTG AGGATCTAAT GGACCCCTAT CTTCAGCATG GGGAACAGGG TATAATGTTC
    ACCACCTTGA AGGCATGTTA CTACCAGATT CAGCGTGAGA AGCTTAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860058.1
    CDS61..5031
    Translation

    Target ORF information:

    RefSeq Version XM_017004569.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004569.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTGAAG
    CTAGGAAACC TGGCAGTGCC AGCTGATGAG AAGTGGAAAG CGGTGTTTGA CGAATGTTGG
    CGCCAACTGG GCTGTGAGGG CCCAGGCCAA GGGGAAGTTT GGAAAGCATG GGACAATATT
    GCTGTGGCCT TGACCACCAG TCGGCGCCAG CACAGAGACA GTCCAGAAAT ACTGAGTCGT
    TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCAC AGCATGTTGG TGCCTTCTCT
    GTGGAGAAAT ATGATGACAT CAGGAAATAT TTAATAAAGG CCTGTGACAC TCCTCTGCAC
    CCCCTGGGCA GGCTTGTGGA GACACTGGTT GCAGTGTATA GAATGACATA CGTGGGCGTA
    GGAGCCAACC GCAGGTTATT GCAGGAGGCT GTAAAGGAGA TTAAGTCCTA TCTTAAGCGA
    ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTGCCTG AAGAAGGCAG CACAATTCCT
    CTCTCTGCTC CTCTGCCAAC CGAAAGGAAG TCTTTTTGCA CTGGGAAGTC AGATTCCCGA
    TCTGAATCAC CAGAGCCAGG TTATGTAGTA ACGAGTTCTG GATTATTGCT TCCTGTGCTG
    CTACCTCGGC TCTACCCACC GCTGTTTATG CTTTATGCTT TGGATAATGA TCGCGAGGAA
    GACATTTACT GGGAATGTGT CCTTCGACTA AATAAGCAGC CAGATATTGC TCTCCTGGGC
    TTTCTTGGGG TGCAGAGGAA ATTTTGGCCA GCAACCTTGT CAATCCTTGG AGAGAGTAAA
    AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CAGTAGAATG TCTGCAGCAG
    ATCAGCACAA CATTTACCCC ATCAGACAAA CTTAAGGTCA TCCAGCAGAC TTTTGAGGAG
    ATCTCTCAGA GTGTCCTGGC GTCACTCCAC GAAGACTTCT TGTGGTCCAT GGATGACTTG
    TTTCCTGTTT TCTTATATGT GGTGCTACGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA
    CACCTCATTG AGGATCTAAT GGACCCCTAT CTTCAGCATG GGGAACAGGG TATAATGTTC
    ACCACCTTGA AGGCATGTTA CTACCAGATT CAGCGTGAGA AGCTTAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36475
    Clone ID Related Accession (Same CDS sequence) XM_017004569.1 , XM_017004569.2
    Accession Version XM_017004569.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4971bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alsin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017004569.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTGAAG
    CTAGGAAACC TGGCAGTGCC AGCTGATGAG AAGTGGAAAG CGGTGTTTGA CGAATGTTGG
    CGCCAACTGG GCTGTGAGGG CCCAGGCCAA GGGGAAGTTT GGAAAGCATG GGACAATATT
    GCTGTGGCCT TGACCACCAG TCGGCGCCAG CACAGAGACA GTCCAGAAAT ACTGAGTCGT
    TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCAC AGCATGTTGG TGCCTTCTCT
    GTGGAGAAAT ATGATGACAT CAGGAAATAT TTAATAAAGG CCTGTGACAC TCCTCTGCAC
    CCCCTGGGCA GGCTTGTGGA GACACTGGTT GCAGTGTATA GAATGACATA CGTGGGCGTA
    GGAGCCAACC GCAGGTTATT GCAGGAGGCT GTAAAGGAGA TTAAGTCCTA TCTTAAGCGA
    ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTGCCTG AAGAAGGCAG CACAATTCCT
    CTCTCTGCTC CTCTGCCAAC CGAAAGGAAG TCTTTTTGCA CTGGGAAGTC AGATTCCCGA
    TCTGAATCAC CAGAGCCAGG TTATGTAGTA ACGAGTTCTG GATTATTGCT TCCTGTGCTG
    CTACCTCGGC TCTACCCACC GCTGTTTATG CTTTATGCTT TGGATAATGA TCGCGAGGAA
    GACATTTACT GGGAATGTGT CCTTCGACTA AATAAGCAGC CAGATATTGC TCTCCTGGGC
    TTTCTTGGGG TGCAGAGGAA ATTTTGGCCA GCAACCTTGT CAATCCTTGG AGAGAGTAAA
    AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CAGTAGAATG TCTGCAGCAG
    ATCAGCACAA CATTTACCCC ATCAGACAAA CTTAAGGTCA TCCAGCAGAC TTTTGAGGAG
    ATCTCTCAGA GTGTCCTGGC GTCACTCCAC GAAGACTTCT TGTGGTCCAT GGATGACTTG
    TTTCCTGTTT TCTTATATGT GGTGCTACGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA
    CACCTCATTG AGGATCTAAT GGACCCCTAT CTTCAGCATG GGGAACAGGG TATAATGTTC
    ACCACCTTGA AGGCATGTTA CTACCAGATT CAGCGTGAGA AGCTTAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860058.1
    CDS136..5106
    Translation

    Target ORF information:

    RefSeq Version XM_017004569.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004569.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTGAAG
    CTAGGAAACC TGGCAGTGCC AGCTGATGAG AAGTGGAAAG CGGTGTTTGA CGAATGTTGG
    CGCCAACTGG GCTGTGAGGG CCCAGGCCAA GGGGAAGTTT GGAAAGCATG GGACAATATT
    GCTGTGGCCT TGACCACCAG TCGGCGCCAG CACAGAGACA GTCCAGAAAT ACTGAGTCGT
    TCACAGACTC AGACACTAGA GAGTTTGGAA TTCATTCCAC AGCATGTTGG TGCCTTCTCT
    GTGGAGAAAT ATGATGACAT CAGGAAATAT TTAATAAAGG CCTGTGACAC TCCTCTGCAC
    CCCCTGGGCA GGCTTGTGGA GACACTGGTT GCAGTGTATA GAATGACATA CGTGGGCGTA
    GGAGCCAACC GCAGGTTATT GCAGGAGGCT GTAAAGGAGA TTAAGTCCTA TCTTAAGCGA
    ATTTTCCAGC TGGTGAGGTT CTTATTTCCT GAGCTGCCTG AAGAAGGCAG CACAATTCCT
    CTCTCTGCTC CTCTGCCAAC CGAAAGGAAG TCTTTTTGCA CTGGGAAGTC AGATTCCCGA
    TCTGAATCAC CAGAGCCAGG TTATGTAGTA ACGAGTTCTG GATTATTGCT TCCTGTGCTG
    CTACCTCGGC TCTACCCACC GCTGTTTATG CTTTATGCTT TGGATAATGA TCGCGAGGAA
    GACATTTACT GGGAATGTGT CCTTCGACTA AATAAGCAGC CAGATATTGC TCTCCTGGGC
    TTTCTTGGGG TGCAGAGGAA ATTTTGGCCA GCAACCTTGT CAATCCTTGG AGAGAGTAAA
    AAGGTTTTGC CAACCACGAA AGATGCTTGT TTTGCCTCAG CAGTAGAATG TCTGCAGCAG
    ATCAGCACAA CATTTACCCC ATCAGACAAA CTTAAGGTCA TCCAGCAGAC TTTTGAGGAG
    ATCTCTCAGA GTGTCCTGGC GTCACTCCAC GAAGACTTCT TGTGGTCCAT GGATGACTTG
    TTTCCTGTTT TCTTATATGT GGTGCTACGG GCCAGGATTA GGAATTTAGG CTCTGAGGTA
    CACCTCATTG AGGATCTAAT GGACCCCTAT CTTCAGCATG GGGAACAGGG TATAATGTTC
    ACCACCTTGA AGGCATGTTA CTACCAGATT CAGCGTGAGA AGCTTAACTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70842
    Clone ID Related Accession (Same CDS sequence) XM_024453024.1 , XM_011511530.2
    Accession Version XM_024453024.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alsin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTGGGGAG AGAATTCTGC TGGCCAGTGT GCAGTAGCCA ACCAGCAGTA TGTGCCGGAA 
    CCAAATCCTG TCAGCATTGC TGATTCTGAG GCCAGCCCTT TGTTAGCAGT CAGGATTTTA
    CAGTTGGCGT GTGGCGAGGA GCACACTCTG GCATTGTCAA TAAGCAGAGA GATTTGGGCA
    TGGGGTACCG GTTGTCAGTT GGGTCTCATT ACCACTGCCT TCCCAGTGAC AAAGCCGCAA
    AAGGTAGAAC ATCTTGCTGG GCGAGTGGTG CTTCAAGTTG CCTGTGGTGC TTTCCACAGC
    TTAGCCCTTG TACAATGCCT CCCTTCCCAG GATCTGAAGC CAGTCCCAGA ACGATGCAAC
    CAGTGCAGCC AGCTCTTGAT TACTATGACT GACAAAGAAG ACCATGTGAT TATATCAGAC
    AGTCATTGTT GCCCATTAGG TGTGACACTG ACAGAATCTC AGGCAGAAAA CCATGCCAGC
    ACTGCTCTCA GCCCCTCCAC TGAAACCCTT GACAGGCAGG AAGAAGTATT TGAGAACACT
    CTTGTAGCAA ATGATCAGTC TGTTGCTACT GAACTGAATG CAGTAAGTGC TCAGATCACA
    AGCAGCGATG CCATGTCCTC TCAACAAAAT GTCATGGGAA CAACTGAAAT TTCCTCTGCC
    AGAAACATAC CATCATACCC TGACACCCAA GCAGTCAATG AATACCTACG GAAACTGTCA
    GATCATTCAG TAAGAGAGGA CTCAGAGCAT GGTGAAAAGC CAGTGCCATC TCAGCCTCTT
    TTAGAAGAAG CAATTCCTAA TCTCCACAGC CCGCCTACCA CAAGCACCTC AGCCCTAAAC
    AGCCTGGTGG TCTCTTGTGC ATCTGCTGTT GGTGTGAGAG TGGCTGCTAC TTATGAAGCT
    GGTGCCTTGT CACTGAAGAA AGTTATGAAC TTTTATAGTA CAACCCCTTG TGAAACTGGA
    GCTCAGGCAG GCAGTAGTGC CATTGGCCCC GAAGGTTTGA AAGATAGCAG GGAAGAACAG
    GTTAAACAGG AATCAATGCA AGGAAAGAAA AGTTCAAGTC TTGTGGATAT CAGAGAAGAA
    GAAACAGAGG GAGGCAGTCG AAGACTCTCC CTCCCTGGAT TGTTGTCACA AGTTTCCCCC
    AGGCTCTTAA GAAAGGCTGC ACGGGTGAAA ACGAGGACAG TGGTTCTGAC CCCCACATAC
    AGTGGAGAAG CAGATGCGCT CCTGCCTTCT CTGAGAACAG AAGTGTGGAC CTGGGGGAAA
    GGGAAGGAAG GGCAGCTGGG GCACGGCGAT GTTCTGCCTA GGCTTCAACC GTTGTGTGTA
    AAATGTCTGG ATGGCAAAGA AGTAATCCAT CTGGAGGCAG GTGGTTACCA TTCTCTTGCA
    CTTACTGCGA AATCCCAGGT TTACTCATGG GGTAGCAATA CCTTTGGTCA ACTTGGGCAT
    TCCGATTTTC CAACAACAGT TCCTCGTCTT GCAAAGATAA GCAGTGAAAA TGGAGTCTGG
    AGCATAGCTG CAGGCAGGGA TTATTCCCTG TTTTTAGTGG ATACAGAAGA CTTCCAGCCT
    GGGTTATATT ACAGTGGCCG ACAGGACCCT ACAGAAGGTG ACAACCTTCC AGAGAATCAC
    AGTGGTTCTA AGACTCCAGT ACTTCTCTCC TGTAGTAAGC TTGGATATAT AAGCAGAGTG
    ACAGCAGGAA AAGATAGCTA TTTAGCCTTG GTGGATAAAA ACATTATGGG GTATATTGCC
    AGTCTCCACG AGTTAGCTAC TACAGAAAGA CGATTCTATT CAAAACTAAG TGATATCAAA
    TCTCAGATTC TCAGGCCTCT TCTCAGTTTA GAAAATTTGG GCACTACAAC TACAGTCCAG
    CTGTTGCAGG AGGTGGCTAG CCGATTCAGC AAGCTGTGTT ACCTCATTGG TCAGCATGGA
    GCCTCATTGA GCAGCTTCCT TCATGGGGTA AAGGAAGCCA GGAGTTTGGT CATCCTGAAG
    CATTCAAGTC TCTTCTTGGA TAGTTATACA GAGTATTGCA CATCTATTAC AAATTTCCTG
    GTTATGGGAG GATTCCAGCT TCTTGCTAAG CCTGCCATTG ATTTCCTAAA TAAAAACCAA
    GAGCTGTTGC AAGATTTGTC AGAAGTGAAT GACGAAAACA CTCAGTTGAT GGAAATACTG
    AATACTTTGT TTTTCTTGCC AATCAGACGA CTTCATAATT ACGCAAAAGT TTTGCTAAAG
    CTTGCTACTT GTTTTGAAGT GGCATCTCCA GAATATCAGA AACTGCAGGA TTCCAGTTCT
    TGTTATGAGT GTCTTGCTCT CCATCTCGGC AGGAAAAGGA AGGAAGCAGA ATACACACTG
    GGCTTCTGGA AGACCTTCCC CGGAAAAATG ACGGATTCCT TGAGGAAGCC AGAGCGTCGA
    CTGCTGTGTG AGAGTAGTAA CCGAGCCCTG TCTCTGCAGC ATGCTGGGAG GTTTTCCGTG
    AATTGGTTCA TTCTCTTTAA TGATGCCCTG GTCCATGCCC AGTTCTCCAC GCACCATGTT
    TTCCCTCTGG CCACGCTGTG GGCAGAGCCA CTGTCTGAAG AAGCTGGTGG TGTGAATGGC
    TTAAAGATAA CTACACCTGA GGAGCAGTTC ACTCTCATTT CATCTACACC CCAGGAAAAG
    ACAAAGTGGC TACGAGCTAT AAGCCAAGCC GTAGATCAGG CTTTGAGAGG GATGTCTGAT
    CTCCCCCCTT ATGGAAGTGG TAGCAGTGTT CAGAGACAGG AACCACCCAT TTCACGCAGT
    GCCAAATATA CTTTCTACAA GGATCCTCGC CTAAAGGATG CCACCTATGA TGGACGCTGG
    CTTTCAGGGA AGCCTCATGG CAGAGGGGTT TTGAAGTGGC CTGATGGAAA GATGTATTCT
    GGCATGTTCA GGAATGGCTT GGAAGATGGG TATGGAGAAT ACAGAATCCC AAACAAGGCA
    ATGAACAAAG AAGACCATTA TGTGGGCCAT TGGAAAGAAG GAAAAATGTG CGGTCAAGGA
    GTCTACAGCT ATGCTTCTGG TGAAGTATTT GAGGGCTGTT TTCAAGATAA TATGCGTCAT
    GGTCATGGTC TTCTACGAAG TGGGAAATTG ACGTCCTCTT CTCCTAGTAT GTTCATTGGC
    CAGTGGGTAA TGGATAAGAA AGCAGGATAT GGTGTCTTTG ATGATATCAC TAGGGGGGAA
    AAGTATATGG GAATGTGGCA AGATGATGTG TGTCAAGGGA ATGGTGTGGT GGTTACCCAG
    TTTGGATTAT ACTACGAGGG CAACTTTCAC CTTAATAAAA TGATGGGAAA TGGGGTTTTG
    CTTTCCGAAG ATGATACTAT CTATGAAGGA GAATTTTCAG ATGACTGGAC TCTTAGTGGA
    AAGGGAACAC TGACTATGCC AAATGGAGAC TACATTGAAG GTTATTTTAG TGGAGAATGG
    GGATCTGGGA TAAAAATCAC TGGAACCTAC TTCAAACCTA GTCTATATGA GAGTGATAAA
    GACAGACCTA AAGTTTTCAG GAAGCTAGGA AACCTGGCAG TGCCAGCTGA TGAGAAGTGG
    AAAGCGGTGT TTGACGAATG TTGGCGCCAA CTGGGCTGTG AGGGCCCAGG CCAAGGGGAA
    GTTTGGAAAG CATGGGACAA TATTGCTGTG GCCTTGACCA CCAGTCGGCG CCAGCACAGA
    GACAGTCCAG AAATACTGAG TCGTTCACAG ACTCAGACAC TAGAGAGTTT GGAATTCATT
    CCACAGCATG TTGGTGCCTT CTCTGTGGAG AAATATGATG ACATCAGGAA ATATTTAATA
    AAGGCCTGTG ACACTCCTCT GCACCCCCTG GGCAGGCTTG TGGAGACACT GGTTGCAGTG
    TATAGAATGA CATACGTGGG CGTAGGAGCC AACCGCAGGT TATTGCAGGA GGCTGTAAAG
    GAGATTAAGT CCTATCTTAA GCGAATTTTC CAGCTGGTGA GGTTCTTATT TCCTGAGCTG
    CCTGAAGAAG GCAGCACAAT TCCTCTCTCT GCTCCTCTGC CAACCGAAAG GAAGTCTTTT
    TGCACTGGGA AGTCAGATTC CCGATCTGAA TCACCAGAGC CAGGTTATGT AGTAACGAGT
    TCTGGATTAT TGCTTCCTGT GCTGCTACCT CGGCTCTACC CACCGCTGTT TATGCTTTAT
    GCTTTGGATA ATGATCGCGA GGAAGACATT TACTGGGAAT GTGTCCTTCG ACTAAATAAG
    CAGCCAGATA TTGCTCTCCT GGGCTTTCTT GGGGTGCAGA GGAAATTTTG GCCAGCAACC
    TTGTCAATCC TTGGAGAGAG TAAAAAGGTT TTGCCAACCA CGAAAGATGC TTGTTTTGCC
    TCAGCAGTAG AATGTCTGCA GCAGATCAGC ACAACATTTA CCCCATCAGA CAAACTTAAG
    GTCATCCAGC AGACTTTTGA GGAGATCTCT CAGAGTGTCC TGGCGTCACT CCACGAAGAC
    TTCTTGTGGT CCATGGATGA CTTGTTTCCT GTTTTCTTAT ATGTGGTGCT ACGGGCCAGG
    ATTAGGAATT TAGGCTCTGA GGTACACCTC ATTGAGGATC TAATGGACCC CTATCTTCAG
    CATGGGGAAC AGGGTATAAT GTTCACCACC TTGAAGGCAT GTTACTACCA GATTCAGCGT
    GAGAAGCTTA ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308792.1
    CDS852..5486
    Translation

    Target ORF information:

    RefSeq Version XM_024453024.1
    Organism Homo sapiens(human)
    Definition Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024453024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTGGGGAG AGAATTCTGC TGGCCAGTGT GCAGTAGCCA ACCAGCAGTA TGTGCCGGAA 
    CCAAATCCTG TCAGCATTGC TGATTCTGAG GCCAGCCCTT TGTTAGCAGT CAGGATTTTA
    CAGTTGGCGT GTGGCGAGGA GCACACTCTG GCATTGTCAA TAAGCAGAGA GATTTGGGCA
    TGGGGTACCG GTTGTCAGTT GGGTCTCATT ACCACTGCCT TCCCAGTGAC AAAGCCGCAA
    AAGGTAGAAC ATCTTGCTGG GCGAGTGGTG CTTCAAGTTG CCTGTGGTGC TTTCCACAGC
    TTAGCCCTTG TACAATGCCT CCCTTCCCAG GATCTGAAGC CAGTCCCAGA ACGATGCAAC
    CAGTGCAGCC AGCTCTTGAT TACTATGACT GACAAAGAAG ACCATGTGAT TATATCAGAC
    AGTCATTGTT GCCCATTAGG TGTGACACTG ACAGAATCTC AGGCAGAAAA CCATGCCAGC
    ACTGCTCTCA GCCCCTCCAC TGAAACCCTT GACAGGCAGG AAGAAGTATT TGAGAACACT
    CTTGTAGCAA ATGATCAGTC TGTTGCTACT GAACTGAATG CAGTAAGTGC TCAGATCACA
    AGCAGCGATG CCATGTCCTC TCAACAAAAT GTCATGGGAA CAACTGAAAT TTCCTCTGCC
    AGAAACATAC CATCATACCC TGACACCCAA GCAGTCAATG AATACCTACG GAAACTGTCA
    GATCATTCAG TAAGAGAGGA CTCAGAGCAT GGTGAAAAGC CAGTGCCATC TCAGCCTCTT
    TTAGAAGAAG CAATTCCTAA TCTCCACAGC CCGCCTACCA CAAGCACCTC AGCCCTAAAC
    AGCCTGGTGG TCTCTTGTGC ATCTGCTGTT GGTGTGAGAG TGGCTGCTAC TTATGAAGCT
    GGTGCCTTGT CACTGAAGAA AGTTATGAAC TTTTATAGTA CAACCCCTTG TGAAACTGGA
    GCTCAGGCAG GCAGTAGTGC CATTGGCCCC GAAGGTTTGA AAGATAGCAG GGAAGAACAG
    GTTAAACAGG AATCAATGCA AGGAAAGAAA AGTTCAAGTC TTGTGGATAT CAGAGAAGAA
    GAAACAGAGG GAGGCAGTCG AAGACTCTCC CTCCCTGGAT TGTTGTCACA AGTTTCCCCC
    AGGCTCTTAA GAAAGGCTGC ACGGGTGAAA ACGAGGACAG TGGTTCTGAC CCCCACATAC
    AGTGGAGAAG CAGATGCGCT CCTGCCTTCT CTGAGAACAG AAGTGTGGAC CTGGGGGAAA
    GGGAAGGAAG GGCAGCTGGG GCACGGCGAT GTTCTGCCTA GGCTTCAACC GTTGTGTGTA
    AAATGTCTGG ATGGCAAAGA AGTAATCCAT CTGGAGGCAG GTGGTTACCA TTCTCTTGCA
    CTTACTGCGA AATCCCAGGT TTACTCATGG GGTAGCAATA CCTTTGGTCA ACTTGGGCAT
    TCCGATTTTC CAACAACAGT TCCTCGTCTT GCAAAGATAA GCAGTGAAAA TGGAGTCTGG
    AGCATAGCTG CAGGCAGGGA TTATTCCCTG TTTTTAGTGG ATACAGAAGA CTTCCAGCCT
    GGGTTATATT ACAGTGGCCG ACAGGACCCT ACAGAAGGTG ACAACCTTCC AGAGAATCAC
    AGTGGTTCTA AGACTCCAGT ACTTCTCTCC TGTAGTAAGC TTGGATATAT AAGCAGAGTG
    ACAGCAGGAA AAGATAGCTA TTTAGCCTTG GTGGATAAAA ACATTATGGG GTATATTGCC
    AGTCTCCACG AGTTAGCTAC TACAGAAAGA CGATTCTATT CAAAACTAAG TGATATCAAA
    TCTCAGATTC TCAGGCCTCT TCTCAGTTTA GAAAATTTGG GCACTACAAC TACAGTCCAG
    CTGTTGCAGG AGGTGGCTAG CCGATTCAGC AAGCTGTGTT ACCTCATTGG TCAGCATGGA
    GCCTCATTGA GCAGCTTCCT TCATGGGGTA AAGGAAGCCA GGAGTTTGGT CATCCTGAAG
    CATTCAAGTC TCTTCTTGGA TAGTTATACA GAGTATTGCA CATCTATTAC AAATTTCCTG
    GTTATGGGAG GATTCCAGCT TCTTGCTAAG CCTGCCATTG ATTTCCTAAA TAAAAACCAA
    GAGCTGTTGC AAGATTTGTC AGAAGTGAAT GACGAAAACA CTCAGTTGAT GGAAATACTG
    AATACTTTGT TTTTCTTGCC AATCAGACGA CTTCATAATT ACGCAAAAGT TTTGCTAAAG
    CTTGCTACTT GTTTTGAAGT GGCATCTCCA GAATATCAGA AACTGCAGGA TTCCAGTTCT
    TGTTATGAGT GTCTTGCTCT CCATCTCGGC AGGAAAAGGA AGGAAGCAGA ATACACACTG
    GGCTTCTGGA AGACCTTCCC CGGAAAAATG ACGGATTCCT TGAGGAAGCC AGAGCGTCGA
    CTGCTGTGTG AGAGTAGTAA CCGAGCCCTG TCTCTGCAGC ATGCTGGGAG GTTTTCCGTG
    AATTGGTTCA TTCTCTTTAA TGATGCCCTG GTCCATGCCC AGTTCTCCAC GCACCATGTT
    TTCCCTCTGG CCACGCTGTG GGCAGAGCCA CTGTCTGAAG AAGCTGGTGG TGTGAATGGC
    TTAAAGATAA CTACACCTGA GGAGCAGTTC ACTCTCATTT CATCTACACC CCAGGAAAAG
    ACAAAGTGGC TACGAGCTAT AAGCCAAGCC GTAGATCAGG CTTTGAGAGG GATGTCTGAT
    CTCCCCCCTT ATGGAAGTGG TAGCAGTGTT CAGAGACAGG AACCACCCAT TTCACGCAGT
    GCCAAATATA CTTTCTACAA GGATCCTCGC CTAAAGGATG CCACCTATGA TGGACGCTGG
    CTTTCAGGGA AGCCTCATGG CAGAGGGGTT TTGAAGTGGC CTGATGGAAA GATGTATTCT
    GGCATGTTCA GGAATGGCTT GGAAGATGGG TATGGAGAAT ACAGAATCCC AAACAAGGCA
    ATGAACAAAG AAGACCATTA TGTGGGCCAT TGGAAAGAAG GAAAAATGTG CGGTCAAGGA
    GTCTACAGCT ATGCTTCTGG TGAAGTATTT GAGGGCTGTT TTCAAGATAA TATGCGTCAT
    GGTCATGGTC TTCTACGAAG TGGGAAATTG ACGTCCTCTT CTCCTAGTAT GTTCATTGGC
    CAGTGGGTAA TGGATAAGAA AGCAGGATAT GGTGTCTTTG ATGATATCAC TAGGGGGGAA
    AAGTATATGG GAATGTGGCA AGATGATGTG TGTCAAGGGA ATGGTGTGGT GGTTACCCAG
    TTTGGATTAT ACTACGAGGG CAACTTTCAC CTTAATAAAA TGATGGGAAA TGGGGTTTTG
    CTTTCCGAAG ATGATACTAT CTATGAAGGA GAATTTTCAG ATGACTGGAC TCTTAGTGGA
    AAGGGAACAC TGACTATGCC AAATGGAGAC TACATTGAAG GTTATTTTAG TGGAGAATGG
    GGATCTGGGA TAAAAATCAC TGGAACCTAC TTCAAACCTA GTCTATATGA GAGTGATAAA
    GACAGACCTA AAGTTTTCAG GAAGCTAGGA AACCTGGCAG TGCCAGCTGA TGAGAAGTGG
    AAAGCGGTGT TTGACGAATG TTGGCGCCAA CTGGGCTGTG AGGGCCCAGG CCAAGGGGAA
    GTTTGGAAAG CATGGGACAA TATTGCTGTG GCCTTGACCA CCAGTCGGCG CCAGCACAGA
    GACAGTCCAG AAATACTGAG TCGTTCACAG ACTCAGACAC TAGAGAGTTT GGAATTCATT
    CCACAGCATG TTGGTGCCTT CTCTGTGGAG AAATATGATG ACATCAGGAA ATATTTAATA
    AAGGCCTGTG ACACTCCTCT GCACCCCCTG GGCAGGCTTG TGGAGACACT GGTTGCAGTG
    TATAGAATGA CATACGTGGG CGTAGGAGCC AACCGCAGGT TATTGCAGGA GGCTGTAAAG
    GAGATTAAGT CCTATCTTAA GCGAATTTTC CAGCTGGTGA GGTTCTTATT TCCTGAGCTG
    CCTGAAGAAG GCAGCACAAT TCCTCTCTCT GCTCCTCTGC CAACCGAAAG GAAGTCTTTT
    TGCACTGGGA AGTCAGATTC CCGATCTGAA TCACCAGAGC CAGGTTATGT AGTAACGAGT
    TCTGGATTAT TGCTTCCTGT GCTGCTACCT CGGCTCTACC CACCGCTGTT TATGCTTTAT
    GCTTTGGATA ATGATCGCGA GGAAGACATT TACTGGGAAT GTGTCCTTCG ACTAAATAAG
    CAGCCAGATA TTGCTCTCCT GGGCTTTCTT GGGGTGCAGA GGAAATTTTG GCCAGCAACC
    TTGTCAATCC TTGGAGAGAG TAAAAAGGTT TTGCCAACCA CGAAAGATGC TTGTTTTGCC
    TCAGCAGTAG AATGTCTGCA GCAGATCAGC ACAACATTTA CCCCATCAGA CAAACTTAAG
    GTCATCCAGC AGACTTTTGA GGAGATCTCT CAGAGTGTCC TGGCGTCACT CCACGAAGAC
    TTCTTGTGGT CCATGGATGA CTTGTTTCCT GTTTTCTTAT ATGTGGTGCT ACGGGCCAGG
    ATTAGGAATT TAGGCTCTGA GGTACACCTC ATTGAGGATC TAATGGACCC CTATCTTCAG
    CATGGGGAAC AGGGTATAAT GTTCACCACC TTGAAGGCAT GTTACTACCA GATTCAGCGT
    GAGAAGCTTA ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70842
    Clone ID Related Accession (Same CDS sequence) XM_024453024.1 , XM_011511530.2
    Accession Version XM_011511530.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4635bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product alsin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511530.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTGGGGAG AGAATTCTGC TGGCCAGTGT GCAGTAGCCA ACCAGCAGTA TGTGCCGGAA 
    CCAAATCCTG TCAGCATTGC TGATTCTGAG GCCAGCCCTT TGTTAGCAGT CAGGATTTTA
    CAGTTGGCGT GTGGCGAGGA GCACACTCTG GCATTGTCAA TAAGCAGAGA GATTTGGGCA
    TGGGGTACCG GTTGTCAGTT GGGTCTCATT ACCACTGCCT TCCCAGTGAC AAAGCCGCAA
    AAGGTAGAAC ATCTTGCTGG GCGAGTGGTG CTTCAAGTTG CCTGTGGTGC TTTCCACAGC
    TTAGCCCTTG TACAATGCCT CCCTTCCCAG GATCTGAAGC CAGTCCCAGA ACGATGCAAC
    CAGTGCAGCC AGCTCTTGAT TACTATGACT GACAAAGAAG ACCATGTGAT TATATCAGAC
    AGTCATTGTT GCCCATTAGG TGTGACACTG ACAGAATCTC AGGCAGAAAA CCATGCCAGC
    ACTGCTCTCA GCCCCTCCAC TGAAACCCTT GACAGGCAGG AAGAAGTATT TGAGAACACT
    CTTGTAGCAA ATGATCAGTC TGTTGCTACT GAACTGAATG CAGTAAGTGC TCAGATCACA
    AGCAGCGATG CCATGTCCTC TCAACAAAAT GTCATGGGAA CAACTGAAAT TTCCTCTGCC
    AGAAACATAC CATCATACCC TGACACCCAA GCAGTCAATG AATACCTACG GAAACTGTCA
    GATCATTCAG TAAGAGAGGA CTCAGAGCAT GGTGAAAAGC CAGTGCCATC TCAGCCTCTT
    TTAGAAGAAG CAATTCCTAA TCTCCACAGC CCGCCTACCA CAAGCACCTC AGCCCTAAAC
    AGCCTGGTGG TCTCTTGTGC ATCTGCTGTT GGTGTGAGAG TGGCTGCTAC TTATGAAGCT
    GGTGCCTTGT CACTGAAGAA AGTTATGAAC TTTTATAGTA CAACCCCTTG TGAAACTGGA
    GCTCAGGCAG GCAGTAGTGC CATTGGCCCC GAAGGTTTGA AAGATAGCAG GGAAGAACAG
    GTTAAACAGG AATCAATGCA AGGAAAGAAA AGTTCAAGTC TTGTGGATAT CAGAGAAGAA
    GAAACAGAGG GAGGCAGTCG AAGACTCTCC CTCCCTGGAT TGTTGTCACA AGTTTCCCCC
    AGGCTCTTAA GAAAGGCTGC ACGGGTGAAA ACGAGGACAG TGGTTCTGAC CCCCACATAC
    AGTGGAGAAG CAGATGCGCT CCTGCCTTCT CTGAGAACAG AAGTGTGGAC CTGGGGGAAA
    GGGAAGGAAG GGCAGCTGGG GCACGGCGAT GTTCTGCCTA GGCTTCAACC GTTGTGTGTA
    AAATGTCTGG ATGGCAAAGA AGTAATCCAT CTGGAGGCAG GTGGTTACCA TTCTCTTGCA
    CTTACTGCGA AATCCCAGGT TTACTCATGG GGTAGCAATA CCTTTGGTCA ACTTGGGCAT
    TCCGATTTTC CAACAACAGT TCCTCGTCTT GCAAAGATAA GCAGTGAAAA TGGAGTCTGG
    AGCATAGCTG CAGGCAGGGA TTATTCCCTG TTTTTAGTGG ATACAGAAGA CTTCCAGCCT
    GGGTTATATT ACAGTGGCCG ACAGGACCCT ACAGAAGGTG ACAACCTTCC AGAGAATCAC
    AGTGGTTCTA AGACTCCAGT ACTTCTCTCC TGTAGTAAGC TTGGATATAT AAGCAGAGTG
    ACAGCAGGAA AAGATAGCTA TTTAGCCTTG GTGGATAAAA ACATTATGGG GTATATTGCC
    AGTCTCCACG AGTTAGCTAC TACAGAAAGA CGATTCTATT CAAAACTAAG TGATATCAAA
    TCTCAGATTC TCAGGCCTCT TCTCAGTTTA GAAAATTTGG GCACTACAAC TACAGTCCAG
    CTGTTGCAGG AGGTGGCTAG CCGATTCAGC AAGCTGTGTT ACCTCATTGG TCAGCATGGA
    GCCTCATTGA GCAGCTTCCT TCATGGGGTA AAGGAAGCCA GGAGTTTGGT CATCCTGAAG
    CATTCAAGTC TCTTCTTGGA TAGTTATACA GAGTATTGCA CATCTATTAC AAATTTCCTG
    GTTATGGGAG GATTCCAGCT TCTTGCTAAG CCTGCCATTG ATTTCCTAAA TAAAAACCAA
    GAGCTGTTGC AAGATTTGTC AGAAGTGAAT GACGAAAACA CTCAGTTGAT GGAAATACTG
    AATACTTTGT TTTTCTTGCC AATCAGACGA CTTCATAATT ACGCAAAAGT TTTGCTAAAG
    CTTGCTACTT GTTTTGAAGT GGCATCTCCA GAATATCAGA AACTGCAGGA TTCCAGTTCT
    TGTTATGAGT GTCTTGCTCT CCATCTCGGC AGGAAAAGGA AGGAAGCAGA ATACACACTG
    GGCTTCTGGA AGACCTTCCC CGGAAAAATG ACGGATTCCT TGAGGAAGCC AGAGCGTCGA
    CTGCTGTGTG AGAGTAGTAA CCGAGCCCTG TCTCTGCAGC ATGCTGGGAG GTTTTCCGTG
    AATTGGTTCA TTCTCTTTAA TGATGCCCTG GTCCATGCCC AGTTCTCCAC GCACCATGTT
    TTCCCTCTGG CCACGCTGTG GGCAGAGCCA CTGTCTGAAG AAGCTGGTGG TGTGAATGGC
    TTAAAGATAA CTACACCTGA GGAGCAGTTC ACTCTCATTT CATCTACACC CCAGGAAAAG
    ACAAAGTGGC TACGAGCTAT AAGCCAAGCC GTAGATCAGG CTTTGAGAGG GATGTCTGAT
    CTCCCCCCTT ATGGAAGTGG TAGCAGTGTT CAGAGACAGG AACCACCCAT TTCACGCAGT
    GCCAAATATA CTTTCTACAA GGATCCTCGC CTAAAGGATG CCACCTATGA TGGACGCTGG
    CTTTCAGGGA AGCCTCATGG CAGAGGGGTT TTGAAGTGGC CTGATGGAAA GATGTATTCT
    GGCATGTTCA GGAATGGCTT GGAAGATGGG TATGGAGAAT ACAGAATCCC AAACAAGGCA
    ATGAACAAAG AAGACCATTA TGTGGGCCAT TGGAAAGAAG GAAAAATGTG CGGTCAAGGA
    GTCTACAGCT ATGCTTCTGG TGAAGTATTT GAGGGCTGTT TTCAAGATAA TATGCGTCAT
    GGTCATGGTC TTCTACGAAG TGGGAAATTG ACGTCCTCTT CTCCTAGTAT GTTCATTGGC
    CAGTGGGTAA TGGATAAGAA AGCAGGATAT GGTGTCTTTG ATGATATCAC TAGGGGGGAA
    AAGTATATGG GAATGTGGCA AGATGATGTG TGTCAAGGGA ATGGTGTGGT GGTTACCCAG
    TTTGGATTAT ACTACGAGGG CAACTTTCAC CTTAATAAAA TGATGGGAAA TGGGGTTTTG
    CTTTCCGAAG ATGATACTAT CTATGAAGGA GAATTTTCAG ATGACTGGAC TCTTAGTGGA
    AAGGGAACAC TGACTATGCC AAATGGAGAC TACATTGAAG GTTATTTTAG TGGAGAATGG
    GGATCTGGGA TAAAAATCAC TGGAACCTAC TTCAAACCTA GTCTATATGA GAGTGATAAA
    GACAGACCTA AAGTTTTCAG GAAGCTAGGA AACCTGGCAG TGCCAGCTGA TGAGAAGTGG
    AAAGCGGTGT TTGACGAATG TTGGCGCCAA CTGGGCTGTG AGGGCCCAGG CCAAGGGGAA
    GTTTGGAAAG CATGGGACAA TATTGCTGTG GCCTTGACCA CCAGTCGGCG CCAGCACAGA
    GACAGTCCAG AAATACTGAG TCGTTCACAG ACTCAGACAC TAGAGAGTTT GGAATTCATT
    CCACAGCATG TTGGTGCCTT CTCTGTGGAG AAATATGATG ACATCAGGAA ATATTTAATA
    AAGGCCTGTG ACACTCCTCT GCACCCCCTG GGCAGGCTTG TGGAGACACT GGTTGCAGTG
    TATAGAATGA CATACGTGGG CGTAGGAGCC AACCGCAGGT TATTGCAGGA GGCTGTAAAG
    GAGATTAAGT CCTATCTTAA GCGAATTTTC CAGCTGGTGA GGTTCTTATT TCCTGAGCTG
    CCTGAAGAAG GCAGCACAAT TCCTCTCTCT GCTCCTCTGC CAACCGAAAG GAAGTCTTTT
    TGCACTGGGA AGTCAGATTC CCGATCTGAA TCACCAGAGC CAGGTTATGT AGTAACGAGT
    TCTGGATTAT TGCTTCCTGT GCTGCTACCT CGGCTCTACC CACCGCTGTT TATGCTTTAT
    GCTTTGGATA ATGATCGCGA GGAAGACATT TACTGGGAAT GTGTCCTTCG ACTAAATAAG
    CAGCCAGATA TTGCTCTCCT GGGCTTTCTT GGGGTGCAGA GGAAATTTTG GCCAGCAACC
    TTGTCAATCC TTGGAGAGAG TAAAAAGGTT TTGCCAACCA CGAAAGATGC TTGTTTTGCC
    TCAGCAGTAG AATGTCTGCA GCAGATCAGC ACAACATTTA CCCCATCAGA CAAACTTAAG
    GTCATCCAGC AGACTTTTGA GGAGATCTCT CAGAGTGTCC TGGCGTCACT CCACGAAGAC
    TTCTTGTGGT CCATGGATGA CTTGTTTCCT GTTTTCTTAT ATGTGGTGCT ACGGGCCAGG
    ATTAGGAATT TAGGCTCTGA GGTACACCTC ATTGAGGATC TAATGGACCC CTATCTTCAG
    CATGGGGAAC AGGGTATAAT GTTCACCACC TTGAAGGCAT GTTACTACCA GATTCAGCGT
    GAGAAGCTTA ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011509832.1
    CDS408..5042
    Translation

    Target ORF information:

    RefSeq Version XM_011511530.2
    Organism Homo sapiens(human)
    Definition Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011511530.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTGGGGAG AGAATTCTGC TGGCCAGTGT GCAGTAGCCA ACCAGCAGTA TGTGCCGGAA 
    CCAAATCCTG TCAGCATTGC TGATTCTGAG GCCAGCCCTT TGTTAGCAGT CAGGATTTTA
    CAGTTGGCGT GTGGCGAGGA GCACACTCTG GCATTGTCAA TAAGCAGAGA GATTTGGGCA
    TGGGGTACCG GTTGTCAGTT GGGTCTCATT ACCACTGCCT TCCCAGTGAC AAAGCCGCAA
    AAGGTAGAAC ATCTTGCTGG GCGAGTGGTG CTTCAAGTTG CCTGTGGTGC TTTCCACAGC
    TTAGCCCTTG TACAATGCCT CCCTTCCCAG GATCTGAAGC CAGTCCCAGA ACGATGCAAC
    CAGTGCAGCC AGCTCTTGAT TACTATGACT GACAAAGAAG ACCATGTGAT TATATCAGAC
    AGTCATTGTT GCCCATTAGG TGTGACACTG ACAGAATCTC AGGCAGAAAA CCATGCCAGC
    ACTGCTCTCA GCCCCTCCAC TGAAACCCTT GACAGGCAGG AAGAAGTATT TGAGAACACT
    CTTGTAGCAA ATGATCAGTC TGTTGCTACT GAACTGAATG CAGTAAGTGC TCAGATCACA
    AGCAGCGATG CCATGTCCTC TCAACAAAAT GTCATGGGAA CAACTGAAAT TTCCTCTGCC
    AGAAACATAC CATCATACCC TGACACCCAA GCAGTCAATG AATACCTACG GAAACTGTCA
    GATCATTCAG TAAGAGAGGA CTCAGAGCAT GGTGAAAAGC CAGTGCCATC TCAGCCTCTT
    TTAGAAGAAG CAATTCCTAA TCTCCACAGC CCGCCTACCA CAAGCACCTC AGCCCTAAAC
    AGCCTGGTGG TCTCTTGTGC ATCTGCTGTT GGTGTGAGAG TGGCTGCTAC TTATGAAGCT
    GGTGCCTTGT CACTGAAGAA AGTTATGAAC TTTTATAGTA CAACCCCTTG TGAAACTGGA
    GCTCAGGCAG GCAGTAGTGC CATTGGCCCC GAAGGTTTGA AAGATAGCAG GGAAGAACAG
    GTTAAACAGG AATCAATGCA AGGAAAGAAA AGTTCAAGTC TTGTGGATAT CAGAGAAGAA
    GAAACAGAGG GAGGCAGTCG AAGACTCTCC CTCCCTGGAT TGTTGTCACA AGTTTCCCCC
    AGGCTCTTAA GAAAGGCTGC ACGGGTGAAA ACGAGGACAG TGGTTCTGAC CCCCACATAC
    AGTGGAGAAG CAGATGCGCT CCTGCCTTCT CTGAGAACAG AAGTGTGGAC CTGGGGGAAA
    GGGAAGGAAG GGCAGCTGGG GCACGGCGAT GTTCTGCCTA GGCTTCAACC GTTGTGTGTA
    AAATGTCTGG ATGGCAAAGA AGTAATCCAT CTGGAGGCAG GTGGTTACCA TTCTCTTGCA
    CTTACTGCGA AATCCCAGGT TTACTCATGG GGTAGCAATA CCTTTGGTCA ACTTGGGCAT
    TCCGATTTTC CAACAACAGT TCCTCGTCTT GCAAAGATAA GCAGTGAAAA TGGAGTCTGG
    AGCATAGCTG CAGGCAGGGA TTATTCCCTG TTTTTAGTGG ATACAGAAGA CTTCCAGCCT
    GGGTTATATT ACAGTGGCCG ACAGGACCCT ACAGAAGGTG ACAACCTTCC AGAGAATCAC
    AGTGGTTCTA AGACTCCAGT ACTTCTCTCC TGTAGTAAGC TTGGATATAT AAGCAGAGTG
    ACAGCAGGAA AAGATAGCTA TTTAGCCTTG GTGGATAAAA ACATTATGGG GTATATTGCC
    AGTCTCCACG AGTTAGCTAC TACAGAAAGA CGATTCTATT CAAAACTAAG TGATATCAAA
    TCTCAGATTC TCAGGCCTCT TCTCAGTTTA GAAAATTTGG GCACTACAAC TACAGTCCAG
    CTGTTGCAGG AGGTGGCTAG CCGATTCAGC AAGCTGTGTT ACCTCATTGG TCAGCATGGA
    GCCTCATTGA GCAGCTTCCT TCATGGGGTA AAGGAAGCCA GGAGTTTGGT CATCCTGAAG
    CATTCAAGTC TCTTCTTGGA TAGTTATACA GAGTATTGCA CATCTATTAC AAATTTCCTG
    GTTATGGGAG GATTCCAGCT TCTTGCTAAG CCTGCCATTG ATTTCCTAAA TAAAAACCAA
    GAGCTGTTGC AAGATTTGTC AGAAGTGAAT GACGAAAACA CTCAGTTGAT GGAAATACTG
    AATACTTTGT TTTTCTTGCC AATCAGACGA CTTCATAATT ACGCAAAAGT TTTGCTAAAG
    CTTGCTACTT GTTTTGAAGT GGCATCTCCA GAATATCAGA AACTGCAGGA TTCCAGTTCT
    TGTTATGAGT GTCTTGCTCT CCATCTCGGC AGGAAAAGGA AGGAAGCAGA ATACACACTG
    GGCTTCTGGA AGACCTTCCC CGGAAAAATG ACGGATTCCT TGAGGAAGCC AGAGCGTCGA
    CTGCTGTGTG AGAGTAGTAA CCGAGCCCTG TCTCTGCAGC ATGCTGGGAG GTTTTCCGTG
    AATTGGTTCA TTCTCTTTAA TGATGCCCTG GTCCATGCCC AGTTCTCCAC GCACCATGTT
    TTCCCTCTGG CCACGCTGTG GGCAGAGCCA CTGTCTGAAG AAGCTGGTGG TGTGAATGGC
    TTAAAGATAA CTACACCTGA GGAGCAGTTC ACTCTCATTT CATCTACACC CCAGGAAAAG
    ACAAAGTGGC TACGAGCTAT AAGCCAAGCC GTAGATCAGG CTTTGAGAGG GATGTCTGAT
    CTCCCCCCTT ATGGAAGTGG TAGCAGTGTT CAGAGACAGG AACCACCCAT TTCACGCAGT
    GCCAAATATA CTTTCTACAA GGATCCTCGC CTAAAGGATG CCACCTATGA TGGACGCTGG
    CTTTCAGGGA AGCCTCATGG CAGAGGGGTT TTGAAGTGGC CTGATGGAAA GATGTATTCT
    GGCATGTTCA GGAATGGCTT GGAAGATGGG TATGGAGAAT ACAGAATCCC AAACAAGGCA
    ATGAACAAAG AAGACCATTA TGTGGGCCAT TGGAAAGAAG GAAAAATGTG CGGTCAAGGA
    GTCTACAGCT ATGCTTCTGG TGAAGTATTT GAGGGCTGTT TTCAAGATAA TATGCGTCAT
    GGTCATGGTC TTCTACGAAG TGGGAAATTG ACGTCCTCTT CTCCTAGTAT GTTCATTGGC
    CAGTGGGTAA TGGATAAGAA AGCAGGATAT GGTGTCTTTG ATGATATCAC TAGGGGGGAA
    AAGTATATGG GAATGTGGCA AGATGATGTG TGTCAAGGGA ATGGTGTGGT GGTTACCCAG
    TTTGGATTAT ACTACGAGGG CAACTTTCAC CTTAATAAAA TGATGGGAAA TGGGGTTTTG
    CTTTCCGAAG ATGATACTAT CTATGAAGGA GAATTTTCAG ATGACTGGAC TCTTAGTGGA
    AAGGGAACAC TGACTATGCC AAATGGAGAC TACATTGAAG GTTATTTTAG TGGAGAATGG
    GGATCTGGGA TAAAAATCAC TGGAACCTAC TTCAAACCTA GTCTATATGA GAGTGATAAA
    GACAGACCTA AAGTTTTCAG GAAGCTAGGA AACCTGGCAG TGCCAGCTGA TGAGAAGTGG
    AAAGCGGTGT TTGACGAATG TTGGCGCCAA CTGGGCTGTG AGGGCCCAGG CCAAGGGGAA
    GTTTGGAAAG CATGGGACAA TATTGCTGTG GCCTTGACCA CCAGTCGGCG CCAGCACAGA
    GACAGTCCAG AAATACTGAG TCGTTCACAG ACTCAGACAC TAGAGAGTTT GGAATTCATT
    CCACAGCATG TTGGTGCCTT CTCTGTGGAG AAATATGATG ACATCAGGAA ATATTTAATA
    AAGGCCTGTG ACACTCCTCT GCACCCCCTG GGCAGGCTTG TGGAGACACT GGTTGCAGTG
    TATAGAATGA CATACGTGGG CGTAGGAGCC AACCGCAGGT TATTGCAGGA GGCTGTAAAG
    GAGATTAAGT CCTATCTTAA GCGAATTTTC CAGCTGGTGA GGTTCTTATT TCCTGAGCTG
    CCTGAAGAAG GCAGCACAAT TCCTCTCTCT GCTCCTCTGC CAACCGAAAG GAAGTCTTTT
    TGCACTGGGA AGTCAGATTC CCGATCTGAA TCACCAGAGC CAGGTTATGT AGTAACGAGT
    TCTGGATTAT TGCTTCCTGT GCTGCTACCT CGGCTCTACC CACCGCTGTT TATGCTTTAT
    GCTTTGGATA ATGATCGCGA GGAAGACATT TACTGGGAAT GTGTCCTTCG ACTAAATAAG
    CAGCCAGATA TTGCTCTCCT GGGCTTTCTT GGGGTGCAGA GGAAATTTTG GCCAGCAACC
    TTGTCAATCC TTGGAGAGAG TAAAAAGGTT TTGCCAACCA CGAAAGATGC TTGTTTTGCC
    TCAGCAGTAG AATGTCTGCA GCAGATCAGC ACAACATTTA CCCCATCAGA CAAACTTAAG
    GTCATCCAGC AGACTTTTGA GGAGATCTCT CAGAGTGTCC TGGCGTCACT CCACGAAGAC
    TTCTTGTGGT CCATGGATGA CTTGTTTCCT GTTTTCTTAT ATGTGGTGCT ACGGGCCAGG
    ATTAGGAATT TAGGCTCTGA GGTACACCTC ATTGAGGATC TAATGGACCC CTATCTTCAG
    CATGGGGAAC AGGGTATAAT GTTCACCACC TTGAAGGCAT GTTACTACCA GATTCAGCGT
    GAGAAGCTTA ACTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70843
    Clone ID Related Accession (Same CDS sequence) XM_017004570.2 , XM_017004570.1
    Accession Version XM_017004570.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product alsin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017004570.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGCCTCCA GGACCTTCTG
    TGGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860059.1
    CDS136..4164
    Translation

    Target ORF information:

    RefSeq Version XM_017004570.2
    Organism Homo sapiens(human)
    Definition Homo sapiens alsin Rho guanine nucleotide exchange factor ALS2 (ALS2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004570.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGCCTCCA GGACCTTCTG
    TGGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70843
    Clone ID Related Accession (Same CDS sequence) XM_017004570.2 , XM_017004570.1
    Accession Version XM_017004570.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product alsin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGCCTCCA GGACCTTCTG
    TGGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016860059.1
    CDS61..4089
    Translation

    Target ORF information:

    RefSeq Version XM_017004570.1
    Organism Homo sapiens(human)
    Definition Homo sapiens ALS2, alsin Rho guanine nucleotide exchange factor (ALS2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004570.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGACTCAA AGAAGAGAAG CTCAACAGAG GCAGAAGGAT CCAAGGAAAG AGGCCTGGTC 
    CATATCTGGC AGGCAGGATC CTTTCCCATA ACACCAGAGA GATTGCCAGG CTGGGGAGGA
    AAGACTGTTT TGCAGGCAGC CCTCGGAGTG AAACATGGAG TTCTTCTGAC TGAAGATGGT
    GAGGTCTACA GCTTTGGGAC TCTTCCCTGG AGAAGTGGAC CAGTGGAGAT TTGTCCAAGT
    AGCCCCATTC TAGAAAATGC CCTGGTTGGG CAATATGTTA TTACTGTGGC AACAGGAAGC
    TTCCATAGTG GAGCAGTGAC AGACAATGGT GTCGCGTACA TGTGGGGAGA GAATTCTGCT
    GGCCAGTGTG CAGTAGCCAA CCAGCAGTAT GTGCCGGAAC CAAATCCTGT CAGCATTGCT
    GATTCTGAGG CCAGCCCTTT GTTAGCAGTC AGGATTTTAC AGTTGGCGTG TGGCGAGGAG
    CACACTCTGG CATTGTCAAT AAGCAGAGAG ATTTGGGCAT GGGGTACCGG TTGTCAGTTG
    GGTCTCATTA CCACTGCCTT CCCAGTGACA AAGCCGCAAA AGGTAGAACA TCTTGCTGGG
    CGAGTGGTGC TTCAAGTTGC CTGTGGTGCT TTCCACAGCT TAGCCCTTGT ACAATGCCTC
    CCTTCCCAGG ATCTGAAGCC AGTCCCAGAA CGATGCAACC AGTGCAGCCA GCTCTTGATT
    ACTATGACTG ACAAAGAAGA CCATGTGATT ATATCAGACA GTCATTGTTG CCCATTAGGT
    GTGACACTGA CAGAATCTCA GGCAGAAAAC CATGCCAGCA CTGCTCTCAG CCCCTCCACT
    GAAACCCTTG ACAGGCAGGA AGAAGTATTT GAGAACACTC TTGTAGCAAA TGATCAGTCT
    GTTGCTACTG AACTGAATGC AGTAAGTGCT CAGATCACAA GCAGCGATGC CATGTCCTCT
    CAACAAAATG TCATGGGAAC AACTGAAATT TCCTCTGCCA GAAACATACC ATCATACCCT
    GACACCCAAG CAGTCAATGA ATACCTACGG AAACTGTCAG ATCATTCAGT AAGAGAGGAC
    TCAGAGCATG GTGAAAAGCC AGTGCCATCT CAGCCTCTTT TAGAAGAAGC AATTCCTAAT
    CTCCACAGCC CGCCTACCAC AAGCACCTCA GCCCTAAACA GCCTGGTGGT CTCTTGTGCA
    TCTGCTGTTG GTGTGAGAGT GGCTGCTACT TATGAAGCTG GTGCCTTGTC ACTGAAGAAA
    GTTATGAACT TTTATAGTAC AACCCCTTGT GAAACTGGAG CTCAGGCAGG CAGTAGTGCC
    ATTGGCCCCG AAGGTTTGAA AGATAGCAGG GAAGAACAGG TTAAACAGGA ATCAATGCAA
    GGAAAGAAAA GTTCAAGTCT TGTGGATATC AGAGAAGAAG AAACAGAGGG AGGCAGTCGA
    AGACTCTCCC TCCCTGGATT GTTGTCACAA GTTTCCCCCA GGCTCTTAAG AAAGGCTGCA
    CGGGTGAAAA CGAGGACAGT GGTTCTGACC CCCACATACA GTGGAGAAGC AGATGCGCTC
    CTGCCTTCTC TGAGAACAGA AGTGTGGACC TGGGGGAAAG GGAAGGAAGG GCAGCTGGGG
    CACGGCGATG TTCTGCCTAG GCTTCAACCG TTGTGTGTAA AATGTCTGGA TGGCAAAGAA
    GTAATCCATC TGGAGGCAGG TGGTTACCAT TCTCTTGCAC TTACTGCGAA ATCCCAGGTT
    TACTCATGGG GTAGCAATAC CTTTGGTCAA CTTGGGCATT CCGATTTTCC AACAACAGTT
    CCTCGTCTTG CAAAGATAAG CAGTGAAAAT GGAGTCTGGA GCATAGCTGC AGGCAGGGAT
    TATTCCCTGT TTTTAGTGGA TACAGAAGAC TTCCAGCCTG GGTTATATTA CAGTGGCCGA
    CAGGACCCTA CAGAAGGTGA CAACCTTCCA GAGAATCACA GTGGTTCTAA GACTCCAGTA
    CTTCTCTCCT GTAGTAAGCT TGGATATATA AGCAGAGTGA CAGCAGGAAA AGATAGCTAT
    TTAGCCTTGG TGGATAAAAA CATTATGGGG TATATTGCCA GTCTCCACGA GTTAGCTACT
    ACAGAAAGAC GATTCTATTC AAAACTAAGT GATATCAAAT CTCAGATTCT CAGGCCTCTT
    CTCAGTTTAG AAAATTTGGG CACTACAACT ACAGTCCAGC TGTTGCAGGA GGTGGCTAGC
    CGATTCAGCA AGCTGTGTTA CCTCATTGGT CAGCATGGAG CCTCATTGAG CAGCTTCCTT
    CATGGGGTAA AGGAAGCCAG GAGTTTGGTC ATCCTGAAGC ATTCAAGTCT CTTCTTGGAT
    AGTTATACAG AGTATTGCAC ATCTATTACA AATTTCCTGG TTATGGGAGG ATTCCAGCTT
    CTTGCTAAGC CTGCCATTGA TTTCCTAAAT AAAAACCAAG AGCTGTTGCA AGATTTGTCA
    GAAGTGAATG ACGAAAACAC TCAGTTGATG GAAATACTGA ATACTTTGTT TTTCTTGCCA
    ATCAGACGAC TTCATAATTA CGCAAAAGTT TTGCTAAAGC TTGCTACTTG TTTTGAAGTG
    GCATCTCCAG AATATCAGAA ACTGCAGGAT TCCAGTTCTT GTTATGAGTG TCTTGCTCTC
    CATCTCGGCA GGAAAAGGAA GGAAGCAGAA TACACACTGG GCTTCTGGAA GACCTTCCCC
    GGAAAAATGA CGGATTCCTT GAGGAAGCCA GAGCGTCGAC TGCTGTGTGA GAGTAGTAAC
    CGAGCCCTGT CTCTGCAGCA TGCTGGGAGG TTTTCCGTGA ATTGGTTCAT TCTCTTTAAT
    GATGCCCTGG TCCATGCCCA GTTCTCCACG CACCATGTTT TCCCTCTGGC CACGCTGTGG
    GCAGAGCCAC TGTCTGAAGA AGCTGGTGGT GTGAATGGCT TAAAGATAAC TACACCTGAG
    GAGCAGTTCA CTCTCATTTC ATCTACACCC CAGGAAAAGA CAAAGTGGCT ACGAGCTATA
    AGCCAAGCCG TAGATCAGGC TTTGAGAGGG ATGTCTGATC TCCCCCCTTA TGGAAGTGGT
    AGCAGTGTTC AGAGACAGGA ACCACCCATT TCACGCAGTG CCAAATATAC TTTCTACAAG
    GATCCTCGCC TAAAGGATGC CACCTATGAT GGACGCTGGC TTTCAGGGAA GCCTCATGGC
    AGAGGGGTTT TGAAGTGGCC TGATGGAAAG ATGTATTCTG GCATGTTCAG GAATGGCTTG
    GAAGATGGGT ATGGAGAATA CAGAATCCCA AACAAGGCAA TGAACAAAGA AGACCATTAT
    GTGGGCCATT GGAAAGAAGG AAAAATGTGC GGTCAAGGAG TCTACAGCTA TGCTTCTGGT
    GAAGTATTTG AGGGCTGTTT TCAAGATAAT ATGCGTCATG GTCATGGTCT TCTACGAAGT
    GGGAAATTGA CGTCCTCTTC TCCTAGTATG TTCATTGGCC AGTGGGTAAT GGATAAGAAA
    GCAGGATATG GTGTCTTTGA TGATATCACT AGGGGGGAAA AGTATATGGG AATGTGGCAA
    GATGATGTGT GTCAAGGGAA TGGTGTGGTG GTTACCCAGT TTGGATTATA CTACGAGGGC
    AACTTTCACC TTAATAAAAT GATGGGAAAT GGGGTTTTGC TTTCCGAAGA TGATACTATC
    TATGAAGGAG AATTTTCAGA TGACTGGACT CTTAGTGGAA AGGGAACACT GACTATGCCA
    AATGGAGACT ACATTGAAGG TTATTTTAGT GGAGAATGGG GATCTGGGAT AAAAATCACT
    GGAACCTACT TCAAACCTAG TCTATATGAG AGTGATAAAG ACAGACCTAA AGTTTTCAGG
    AAGCTAGGAA ACCTGGCAGT GCCAGCTGAT GAGAAGTGGA AAGCGGTGTT TGACGAATGT
    TGGCGCCAAC TGGGCTGTGA GGGCCCAGGC CAAGGGGAAG TTTGGAAAGC ATGGGACAAT
    ATTGCTGTGG CCTTGACCAC CAGTCGGCGC CAGCACAGAG ACAGCCTCCA GGACCTTCTG
    TGGCAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.