COL20A1 cDNA ORF clone, Homo sapiens(human)

The following COL20A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL20A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu03573 NM_020882.3
Latest version!
Homo sapiens collagen type XX alpha 1 chain (COL20A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
OHu70752 XM_011528939.1
Latest version!
Homo sapiens collagen type XX alpha 1 chain (COL20A1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu70753 XM_011528940.1
Latest version!
Homo sapiens collagen type XX alpha 1 chain (COL20A1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $643.30
$919.00
OHu70750 XM_011528937.1
Latest version!
Homo sapiens collagen type XX alpha 1 chain (COL20A1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu70751 XM_011528938.1
Latest version!
Homo sapiens collagen type XX alpha 1 chain (COL20A1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu03573 NM_020882.2 Homo sapiens collagen type XX alpha 1 chain (COL20A1), mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$274.50-$384.30
$549.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu03573
    Clone ID Related Accession (Same CDS sequence) NM_020882.2 , NM_020882.3
    Accession Version NM_020882.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-16
    Organism Homo sapiens(human)
    Product collagen alpha-1(XX) chain precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC043183.1, BC041767.1, AL121827.34 and AB040943.1. On Aug 18, 2006 this sequence version replaced NM_020882.1. ##Evidence-Data-START## Transcript exon combination :: BC043183.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCTCCG GAGACCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
    CTGGGAAGAG AGCAAGTTCA AGCAAGCGGT CTCCTGAGGC TGGCTGTGCT GCCTGAGGAC
    CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG
    AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC
    ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC
    TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC
    CTGGACAGGA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC
    ACGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG
    GATCCGCGCA CTCCTGCCGG CCCCCAGTTC CGCTGCCTGC CCCCCGTGCC TGCTGACATG
    GTCTTCCTGG TGGACGGGTC CTGGAGCATT GGCCACAGTC ACTTCCAGCA GGTCAAGGAC
    TTCCTGGCCA GTGTCATCGC ACCCTTTGAA ATCGGGCCGG ATAAGGTCCA AGTAGGCCTG
    ACTCAGTACA GCGGGGATGC TCAGACTGAG TGGGACCTGA ACTCCCTCAG CACCAAGGAA
    CAGGTGCTGG CAGCTGTGCG CCGCCTCCGC TACAAGGGGG GGAACACGTT CACAGGCCTT
    GCCCTGACCC ACGTGCTGGG GCAGAACCTG CAGCCGGCGG CTGGCCTCCG TCCAGAGGCA
    GCCAAGGTGG TGATTCTGGT GACGGACGGC AAGTCCCAGG ACGATGTGCA CACTGCTGCC
    CGTGTCCTCA AGGACCTGGG CGTGAACGTC TTCGCTGTGG GTGTGAAGAA CGCCGATGAG
    GCTGAGCTGA GGCTCCTGGC GTCCCCGCCG AGGGACATCA CCGTCCACAG CGTGCTGGAC
    TTCCTGCAGC TCGGCGCGCT GGCTGGCCTG CTCAGCCGTC TCATCTGCCA GAGGCTCCAG
    GGTGGGAGCC CGCGGCAGGG CCCAGCAGCG GCTCCAGCCC TGGACACCCT CCCTGCCCCC
    ACCAGCCTGG TCCTGAGCCA GGTGACCTCC TCCAGCATCC GCCTGTCCTG GACTCCAGCC
    CCCCGGCACC CCCTCAAGTA TCTGATCGTT TGGCGAGCCT CTAGAGGTGG CACCCCCAGG
    GAGGTGGTGG TGGAGGGACC CGCCGCCTCC ACGGAGCTGC ACAACCTGGC CTCCCGCACA
    GAGTACCTGG TCTCCGTGTT CCCCATCTAT GAGGGCGGGG TTGGCGAAGG CCTGCGGGGC
    CTGGTGACCA CAGCACCTCT GCCTCCGCCC CGGGCGCTGA CCCTGGCCGC AGTGACGCCC
    AGAACCGTCC ACCTCACCTG GCAGCCCTCG GCCGGGGCCA CCCACTACCT GGTGCGATGT
    TCTCCTGCTT CCCCCAAGGG TGAAGAGGAG GAGCGAGAGG TGCAGGTCGG GCGGCCCGAG
    GTGCTGCTGG ATGGCCTGGA ACCTGGCAGG GACTATGAGG TCTCGGTGCA GAGCCTGCGA
    GGCCCTGAGG GCAGCGAGGC CCGGGGCATC CGTGCCAGGA CCCCCACCCT GGCCCCCCCG
    AGACACCTGG GCTTCTCAGA CGTGAGCCAC GACGCGGCAC GAGTGTTCTG GGAGGGTGCC
    CCGAGGCCTG TGCGCCTGGT CAGGGTCACC TATGTGTCCA GCGAGGGTGG ACACTCGGGG
    CAGACAGAGG CTCCTGGGAA CGCCACCTCG GCCACGCTGG GGCCTCTCTC TTCCTCCACC
    ACCTACACTG TCCGTGTCAC CTGCCTCTAC CCTGGGGGTG GCTCCTCTAC GCTGACTGGC
    CGGGTGACCA CCAAGAAAGC TCCCAGCCCA AGCCAGCTGT CCATGACGGA GCTGCCAGGG
    GATGCAGTCC AGCTGGCGTG GGTGGCCGCA GCCCCGTCTG GCGTGCTTGT CTACCAGATC
    ACGTGGACGC CCCTGGGAGA GGGGAAGGCT CACGAGATCT CTGTCCCAGG GAACCTCGGC
    ACGGCCGTCC TGCCTGGCCT AGGGAGGCAC ACAGAGTACG ACGTCACCAT CTTGGCCTAC
    TACAGGGACG GGGCCCGCAG TGACCCTGTG TCCCTCCGCT ATACCCCCTC CACGGTGAGC
    AGGAGCCCAC CCTCCAACCT GGCCCTGGCC TCGGAGACCC CCGACAGCCT GCAGGTCAGC
    TGGACGCCCC CGCTTGGCCG CGTGCTCCAT TACTGGCTCA CCTACGCCCC CGCCTCTGGC
    TTGGGACCCG AGAAATCCGT CTCTGTGCCA GGAGCCAGGA GCCACGTGAC ACTGCCCGAC
    CTGCAGGCAG CCACGAAGTA CAGGGTCCTG GTCTCAGCTA TCTATGCAGC AGGCAGGAGT
    GAGGCTGTGT CTGCCACGGG CCAGACAGCC TGCCCAGCCC TCCGCCCTGA CGGCTCCCTC
    CCAGGGTTTG ACCTGATGGT GGCCTTCAGC CTGGTGGAAA AGGCTTATGC GTCCATCCGG
    GGCGTGGCCA TGGAGCCCTC TGCCTTCGGT GGGACCCCGA CCTTCACGCT CTTCAAGGAC
    GCCCAGCTGA CAAGACGGGT CAGTGACGTC TACCCAGCCC CCCTACCTCC AGAGCACACC
    ATCGTCTTCC TTGTGCGCCT ACTTCCCGAG ACACCCCGTG AGGCCTTCGC GCTGTGGCAG
    ATGACAGCCG AGGACTTCCA GCCCCTCCTT GGGGTTCTGC TGGATGCCGG GAAGAAGTCC
    CTGACCTACT TCCACCGTGA CCCCAGGGCT GCCTTGCAGG AGGCCACCTT CGACCCGCAG
    GAAGTGAGGA AGATTTTCTT CGGGAGCTTC CACAAGGTGC ACGTGGCTGT GGGCCGCTCC
    AAGGTCAGGC TCTATGTGGA CTGCCGGAAG GTGGCTGAGC GGCCCCTTGG GGAGATGGGC
    AGCCCACCCG CTGCGGGCTT CGTCACGCTG GGGAGGCTGG CCAAGGCCAG GGGCCCCCGG
    AGCAGTTCGG CCGCGTTTCA GCTCCAGATG CTGCAGATCG TGTGCAGTGA CACCTGGGCC
    GATGAGGACC GGTGCTGTGA GCTCCCTGCC TCGAGGGATG GAGAGACCTG CCCCGCCTTC
    GTGTCTGCCT GTTCCTGTTC CTCAGAGACC CCTGGGCCCC CAGGACCTCA AGGACCCCCA
    GGCCTCCCTG GGAGGAATGG CACCCCAGGA GAGCAGGGCT TCCCAGGGCC CAGGGGTCCA
    CCAGGGGTCA AAGGAGAGAA GGGAGACCAT GGGCTTCCAG GCTTGCAGGG CCACCCCGGC
    CACCAGGGCA TCCCCGGGAG AGTTGGCCTC CAGGGACCAA AGGGAATGAG AGGCCTGGAG
    GGAACTGCTG GCCTGCCTGG ACCCCCTGGC CCCAGGGGGT TCCAGGGCAT GGCAGGGGCC
    AGGGGCACTA GTGGAGAGCG AGGACCTCCA GGGACCGTGG GGCCCACAGG ACTGCCAGGG
    CCCAAAGGGG AACGAGGAGA GAAGGGCGAG CCGCAGTCCC TTGCCACCCT CTACCAGCTT
    GTGAGCCAGG CCTCACACGT GTCAAAGTTC GACTCCTTCC ACGAGAACAC CAGGCCCCCC
    ATGCCCATCT TGGAGCAGAA GCTGGAGCCG GGCACTGAGC CCCTGGGGTC CCCTGGCACC
    CGCAGCAAGG CCCTGGTTCC TGGAGAATGG GGGCGTGGTG GCCGCCACCT TGAGGGCAGA
    GGGGAGCCTG GAGCTGTTGG TCAGATGGGC AGCCCTGGGC AGCAGGGGGC TAGCACCCAG
    GGCCTCTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065933.2
    CDS101..3955
    Translation

    Target ORF information:

    RefSeq Version NM_020882.2
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type XX alpha 1 chain (COL20A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020882.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCTCCG GAGACCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
    CTGGGAAGAG AGCAAGTTCA AGCAAGCGGT CTCCTGAGGC TGGCTGTGCT GCCTGAGGAC
    CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG
    AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC
    ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC
    TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC
    CTGGACAGGA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC
    ACGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG
    GATCCGCGCA CTCCTGCCGG CCCCCAGTTC CGCTGCCTGC CCCCCGTGCC TGCTGACATG
    GTCTTCCTGG TGGACGGGTC CTGGAGCATT GGCCACAGTC ACTTCCAGCA GGTCAAGGAC
    TTCCTGGCCA GTGTCATCGC ACCCTTTGAA ATCGGGCCGG ATAAGGTCCA AGTAGGCCTG
    ACTCAGTACA GCGGGGATGC TCAGACTGAG TGGGACCTGA ACTCCCTCAG CACCAAGGAA
    CAGGTGCTGG CAGCTGTGCG CCGCCTCCGC TACAAGGGGG GGAACACGTT CACAGGCCTT
    GCCCTGACCC ACGTGCTGGG GCAGAACCTG CAGCCGGCGG CTGGCCTCCG TCCAGAGGCA
    GCCAAGGTGG TGATTCTGGT GACGGACGGC AAGTCCCAGG ACGATGTGCA CACTGCTGCC
    CGTGTCCTCA AGGACCTGGG CGTGAACGTC TTCGCTGTGG GTGTGAAGAA CGCCGATGAG
    GCTGAGCTGA GGCTCCTGGC GTCCCCGCCG AGGGACATCA CCGTCCACAG CGTGCTGGAC
    TTCCTGCAGC TCGGCGCGCT GGCTGGCCTG CTCAGCCGTC TCATCTGCCA GAGGCTCCAG
    GGTGGGAGCC CGCGGCAGGG CCCAGCAGCG GCTCCAGCCC TGGACACCCT CCCTGCCCCC
    ACCAGCCTGG TCCTGAGCCA GGTGACCTCC TCCAGCATCC GCCTGTCCTG GACTCCAGCC
    CCCCGGCACC CCCTCAAGTA TCTGATCGTT TGGCGAGCCT CTAGAGGTGG CACCCCCAGG
    GAGGTGGTGG TGGAGGGACC CGCCGCCTCC ACGGAGCTGC ACAACCTGGC CTCCCGCACA
    GAGTACCTGG TCTCCGTGTT CCCCATCTAT GAGGGCGGGG TTGGCGAAGG CCTGCGGGGC
    CTGGTGACCA CAGCACCTCT GCCTCCGCCC CGGGCGCTGA CCCTGGCCGC AGTGACGCCC
    AGAACCGTCC ACCTCACCTG GCAGCCCTCG GCCGGGGCCA CCCACTACCT GGTGCGATGT
    TCTCCTGCTT CCCCCAAGGG TGAAGAGGAG GAGCGAGAGG TGCAGGTCGG GCGGCCCGAG
    GTGCTGCTGG ATGGCCTGGA ACCTGGCAGG GACTATGAGG TCTCGGTGCA GAGCCTGCGA
    GGCCCTGAGG GCAGCGAGGC CCGGGGCATC CGTGCCAGGA CCCCCACCCT GGCCCCCCCG
    AGACACCTGG GCTTCTCAGA CGTGAGCCAC GACGCGGCAC GAGTGTTCTG GGAGGGTGCC
    CCGAGGCCTG TGCGCCTGGT CAGGGTCACC TATGTGTCCA GCGAGGGTGG ACACTCGGGG
    CAGACAGAGG CTCCTGGGAA CGCCACCTCG GCCACGCTGG GGCCTCTCTC TTCCTCCACC
    ACCTACACTG TCCGTGTCAC CTGCCTCTAC CCTGGGGGTG GCTCCTCTAC GCTGACTGGC
    CGGGTGACCA CCAAGAAAGC TCCCAGCCCA AGCCAGCTGT CCATGACGGA GCTGCCAGGG
    GATGCAGTCC AGCTGGCGTG GGTGGCCGCA GCCCCGTCTG GCGTGCTTGT CTACCAGATC
    ACGTGGACGC CCCTGGGAGA GGGGAAGGCT CACGAGATCT CTGTCCCAGG GAACCTCGGC
    ACGGCCGTCC TGCCTGGCCT AGGGAGGCAC ACAGAGTACG ACGTCACCAT CTTGGCCTAC
    TACAGGGACG GGGCCCGCAG TGACCCTGTG TCCCTCCGCT ATACCCCCTC CACGGTGAGC
    AGGAGCCCAC CCTCCAACCT GGCCCTGGCC TCGGAGACCC CCGACAGCCT GCAGGTCAGC
    TGGACGCCCC CGCTTGGCCG CGTGCTCCAT TACTGGCTCA CCTACGCCCC CGCCTCTGGC
    TTGGGACCCG AGAAATCCGT CTCTGTGCCA GGAGCCAGGA GCCACGTGAC ACTGCCCGAC
    CTGCAGGCAG CCACGAAGTA CAGGGTCCTG GTCTCAGCTA TCTATGCAGC AGGCAGGAGT
    GAGGCTGTGT CTGCCACGGG CCAGACAGCC TGCCCAGCCC TCCGCCCTGA CGGCTCCCTC
    CCAGGGTTTG ACCTGATGGT GGCCTTCAGC CTGGTGGAAA AGGCTTATGC GTCCATCCGG
    GGCGTGGCCA TGGAGCCCTC TGCCTTCGGT GGGACCCCGA CCTTCACGCT CTTCAAGGAC
    GCCCAGCTGA CAAGACGGGT CAGTGACGTC TACCCAGCCC CCCTACCTCC AGAGCACACC
    ATCGTCTTCC TTGTGCGCCT ACTTCCCGAG ACACCCCGTG AGGCCTTCGC GCTGTGGCAG
    ATGACAGCCG AGGACTTCCA GCCCCTCCTT GGGGTTCTGC TGGATGCCGG GAAGAAGTCC
    CTGACCTACT TCCACCGTGA CCCCAGGGCT GCCTTGCAGG AGGCCACCTT CGACCCGCAG
    GAAGTGAGGA AGATTTTCTT CGGGAGCTTC CACAAGGTGC ACGTGGCTGT GGGCCGCTCC
    AAGGTCAGGC TCTATGTGGA CTGCCGGAAG GTGGCTGAGC GGCCCCTTGG GGAGATGGGC
    AGCCCACCCG CTGCGGGCTT CGTCACGCTG GGGAGGCTGG CCAAGGCCAG GGGCCCCCGG
    AGCAGTTCGG CCGCGTTTCA GCTCCAGATG CTGCAGATCG TGTGCAGTGA CACCTGGGCC
    GATGAGGACC GGTGCTGTGA GCTCCCTGCC TCGAGGGATG GAGAGACCTG CCCCGCCTTC
    GTGTCTGCCT GTTCCTGTTC CTCAGAGACC CCTGGGCCCC CAGGACCTCA AGGACCCCCA
    GGCCTCCCTG GGAGGAATGG CACCCCAGGA GAGCAGGGCT TCCCAGGGCC CAGGGGTCCA
    CCAGGGGTCA AAGGAGAGAA GGGAGACCAT GGGCTTCCAG GCTTGCAGGG CCACCCCGGC
    CACCAGGGCA TCCCCGGGAG AGTTGGCCTC CAGGGACCAA AGGGAATGAG AGGCCTGGAG
    GGAACTGCTG GCCTGCCTGG ACCCCCTGGC CCCAGGGGGT TCCAGGGCAT GGCAGGGGCC
    AGGGGCACTA GTGGAGAGCG AGGACCTCCA GGGACCGTGG GGCCCACAGG ACTGCCAGGG
    CCCAAAGGGG AACGAGGAGA GAAGGGCGAG CCGCAGTCCC TTGCCACCCT CTACCAGCTT
    GTGAGCCAGG CCTCACACGT GTCAAAGTTC GACTCCTTCC ACGAGAACAC CAGGCCCCCC
    ATGCCCATCT TGGAGCAGAA GCTGGAGCCG GGCACTGAGC CCCTGGGGTC CCCTGGCACC
    CGCAGCAAGG CCCTGGTTCC TGGAGAATGG GGGCGTGGTG GCCGCCACCT TGAGGGCAGA
    GGGGAGCCTG GAGCTGTTGG TCAGATGGGC AGCCCTGGGC AGCAGGGGGC TAGCACCCAG
    GGCCTCTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu03573
    Clone ID Related Accession (Same CDS sequence) NM_020882.2 , NM_020882.3
    Accession Version NM_020882.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3855bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-06
    Organism Homo sapiens(human)
    Product collagen alpha-1(XX) chain precursor
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC043183.1, BC041767.1, AL121827.34 and AB040943.1. On Jun 2, 2019 this sequence version replaced NM_020882.2. ##Evidence-Data-START## Transcript exon combination :: BC043183.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2157437 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCTCCG GAGACCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
    CTGGGAAGAG AGCAAGTTCA AGCAAGCGGT CTCCTGAGGC TGGCTGTGCT GCCTGAGGAC
    CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG
    AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC
    ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC
    TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC
    CTGGACAGGA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC
    ACGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG
    GATCCGCGCA CTCCTGCCGG CCCCCAGTTC CGCTGCCTGC CCCCCGTGCC TGCTGACATG
    GTCTTCCTGG TGGACGGGTC CTGGAGCATT GGCCACAGTC ACTTCCAGCA GGTCAAGGAC
    TTCCTGGCCA GTGTCATCGC ACCCTTTGAA ATCGGGCCGG ATAAGGTCCA AGTAGGCCTG
    ACTCAGTACA GCGGGGATGC TCAGACTGAG TGGGACCTGA ACTCCCTCAG CACCAAGGAA
    CAGGTGCTGG CAGCTGTGCG CCGCCTCCGC TACAAGGGGG GGAACACGTT CACAGGCCTT
    GCCCTGACCC ACGTGCTGGG GCAGAACCTG CAGCCGGCGG CTGGCCTCCG TCCAGAGGCA
    GCCAAGGTGG TGATTCTGGT GACGGACGGC AAGTCCCAGG ACGATGTGCA CACTGCTGCC
    CGTGTCCTCA AGGACCTGGG CGTGAACGTC TTCGCTGTGG GTGTGAAGAA CGCCGATGAG
    GCTGAGCTGA GGCTCCTGGC GTCCCCGCCG AGGGACATCA CCGTCCACAG CGTGCTGGAC
    TTCCTGCAGC TCGGCGCGCT GGCTGGCCTG CTCAGCCGTC TCATCTGCCA GAGGCTCCAG
    GGTGGGAGCC CGCGGCAGGG CCCAGCAGCG GCTCCAGCCC TGGACACCCT CCCTGCCCCC
    ACCAGCCTGG TCCTGAGCCA GGTGACCTCC TCCAGCATCC GCCTGTCCTG GACTCCAGCC
    CCCCGGCACC CCCTCAAGTA TCTGATCGTT TGGCGAGCCT CTAGAGGTGG CACCCCCAGG
    GAGGTGGTGG TGGAGGGACC CGCCGCCTCC ACGGAGCTGC ACAACCTGGC CTCCCGCACA
    GAGTACCTGG TCTCCGTGTT CCCCATCTAT GAGGGCGGGG TTGGCGAAGG CCTGCGGGGC
    CTGGTGACCA CAGCACCTCT GCCTCCGCCC CGGGCGCTGA CCCTGGCCGC AGTGACGCCC
    AGAACCGTCC ACCTCACCTG GCAGCCCTCG GCCGGGGCCA CCCACTACCT GGTGCGATGT
    TCTCCTGCTT CCCCCAAGGG TGAAGAGGAG GAGCGAGAGG TGCAGGTCGG GCGGCCCGAG
    GTGCTGCTGG ATGGCCTGGA ACCTGGCAGG GACTATGAGG TCTCGGTGCA GAGCCTGCGA
    GGCCCTGAGG GCAGCGAGGC CCGGGGCATC CGTGCCAGGA CCCCCACCCT GGCCCCCCCG
    AGACACCTGG GCTTCTCAGA CGTGAGCCAC GACGCGGCAC GAGTGTTCTG GGAGGGTGCC
    CCGAGGCCTG TGCGCCTGGT CAGGGTCACC TATGTGTCCA GCGAGGGTGG ACACTCGGGG
    CAGACAGAGG CTCCTGGGAA CGCCACCTCG GCCACGCTGG GGCCTCTCTC TTCCTCCACC
    ACCTACACTG TCCGTGTCAC CTGCCTCTAC CCTGGGGGTG GCTCCTCTAC GCTGACTGGC
    CGGGTGACCA CCAAGAAAGC TCCCAGCCCA AGCCAGCTGT CCATGACGGA GCTGCCAGGG
    GATGCAGTCC AGCTGGCGTG GGTGGCCGCA GCCCCGTCTG GCGTGCTTGT CTACCAGATC
    ACGTGGACGC CCCTGGGAGA GGGGAAGGCT CACGAGATCT CTGTCCCAGG GAACCTCGGC
    ACGGCCGTCC TGCCTGGCCT AGGGAGGCAC ACAGAGTACG ACGTCACCAT CTTGGCCTAC
    TACAGGGACG GGGCCCGCAG TGACCCTGTG TCCCTCCGCT ATACCCCCTC CACGGTGAGC
    AGGAGCCCAC CCTCCAACCT GGCCCTGGCC TCGGAGACCC CCGACAGCCT GCAGGTCAGC
    TGGACGCCCC CGCTTGGCCG CGTGCTCCAT TACTGGCTCA CCTACGCCCC CGCCTCTGGC
    TTGGGACCCG AGAAATCCGT CTCTGTGCCA GGAGCCAGGA GCCACGTGAC ACTGCCCGAC
    CTGCAGGCAG CCACGAAGTA CAGGGTCCTG GTCTCAGCTA TCTATGCAGC AGGCAGGAGT
    GAGGCTGTGT CTGCCACGGG CCAGACAGCC TGCCCAGCCC TCCGCCCTGA CGGCTCCCTC
    CCAGGGTTTG ACCTGATGGT GGCCTTCAGC CTGGTGGAAA AGGCTTATGC GTCCATCCGG
    GGCGTGGCCA TGGAGCCCTC TGCCTTCGGT GGGACCCCGA CCTTCACGCT CTTCAAGGAC
    GCCCAGCTGA CAAGACGGGT CAGTGACGTC TACCCAGCCC CCCTACCTCC AGAGCACACC
    ATCGTCTTCC TTGTGCGCCT ACTTCCCGAG ACACCCCGTG AGGCCTTCGC GCTGTGGCAG
    ATGACAGCCG AGGACTTCCA GCCCCTCCTT GGGGTTCTGC TGGATGCCGG GAAGAAGTCC
    CTGACCTACT TCCACCGTGA CCCCAGGGCT GCCTTGCAGG AGGCCACCTT CGACCCGCAG
    GAAGTGAGGA AGATTTTCTT CGGGAGCTTC CACAAGGTGC ACGTGGCTGT GGGCCGCTCC
    AAGGTCAGGC TCTATGTGGA CTGCCGGAAG GTGGCTGAGC GGCCCCTTGG GGAGATGGGC
    AGCCCACCCG CTGCGGGCTT CGTCACGCTG GGGAGGCTGG CCAAGGCCAG GGGCCCCCGG
    AGCAGTTCGG CCGCGTTTCA GCTCCAGATG CTGCAGATCG TGTGCAGTGA CACCTGGGCC
    GATGAGGACC GGTGCTGTGA GCTCCCTGCC TCGAGGGATG GAGAGACCTG CCCCGCCTTC
    GTGTCTGCCT GTTCCTGTTC CTCAGAGACC CCTGGGCCCC CAGGACCTCA AGGACCCCCA
    GGCCTCCCTG GGAGGAATGG CACCCCAGGA GAGCAGGGCT TCCCAGGGCC CAGGGGTCCA
    CCAGGGGTCA AAGGAGAGAA GGGAGACCAT GGGCTTCCAG GCTTGCAGGG CCACCCCGGC
    CACCAGGGCA TCCCCGGGAG AGTTGGCCTC CAGGGACCAA AGGGAATGAG AGGCCTGGAG
    GGAACTGCTG GCCTGCCTGG ACCCCCTGGC CCCAGGGGGT TCCAGGGCAT GGCAGGGGCC
    AGGGGCACTA GTGGAGAGCG AGGACCTCCA GGGACCGTGG GGCCCACAGG ACTGCCAGGG
    CCCAAAGGGG AACGAGGAGA GAAGGGCGAG CCGCAGTCCC TTGCCACCCT CTACCAGCTT
    GTGAGCCAGG CCTCACACGT GTCAAAGTTC GACTCCTTCC ACGAGAACAC CAGGCCCCCC
    ATGCCCATCT TGGAGCAGAA GCTGGAGCCG GGCACTGAGC CCCTGGGGTC CCCTGGCACC
    CGCAGCAAGG CCCTGGTTCC TGGAGAATGG GGGCGTGGTG GCCGCCACCT TGAGGGCAGA
    GGGGAGCCTG GAGCTGTTGG TCAGATGGGC AGCCCTGGGC AGCAGGGGGC TAGCACCCAG
    GGCCTCTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065933.2
    CDS101..3955
    Translation

    Target ORF information:

    RefSeq Version NM_020882.3
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type XX alpha 1 chain (COL20A1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020882.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCTCCG GAGACCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
    CTGGGAAGAG AGCAAGTTCA AGCAAGCGGT CTCCTGAGGC TGGCTGTGCT GCCTGAGGAC
    CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG
    AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC
    ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC
    TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC
    CTGGACAGGA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC
    ACGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG
    GATCCGCGCA CTCCTGCCGG CCCCCAGTTC CGCTGCCTGC CCCCCGTGCC TGCTGACATG
    GTCTTCCTGG TGGACGGGTC CTGGAGCATT GGCCACAGTC ACTTCCAGCA GGTCAAGGAC
    TTCCTGGCCA GTGTCATCGC ACCCTTTGAA ATCGGGCCGG ATAAGGTCCA AGTAGGCCTG
    ACTCAGTACA GCGGGGATGC TCAGACTGAG TGGGACCTGA ACTCCCTCAG CACCAAGGAA
    CAGGTGCTGG CAGCTGTGCG CCGCCTCCGC TACAAGGGGG GGAACACGTT CACAGGCCTT
    GCCCTGACCC ACGTGCTGGG GCAGAACCTG CAGCCGGCGG CTGGCCTCCG TCCAGAGGCA
    GCCAAGGTGG TGATTCTGGT GACGGACGGC AAGTCCCAGG ACGATGTGCA CACTGCTGCC
    CGTGTCCTCA AGGACCTGGG CGTGAACGTC TTCGCTGTGG GTGTGAAGAA CGCCGATGAG
    GCTGAGCTGA GGCTCCTGGC GTCCCCGCCG AGGGACATCA CCGTCCACAG CGTGCTGGAC
    TTCCTGCAGC TCGGCGCGCT GGCTGGCCTG CTCAGCCGTC TCATCTGCCA GAGGCTCCAG
    GGTGGGAGCC CGCGGCAGGG CCCAGCAGCG GCTCCAGCCC TGGACACCCT CCCTGCCCCC
    ACCAGCCTGG TCCTGAGCCA GGTGACCTCC TCCAGCATCC GCCTGTCCTG GACTCCAGCC
    CCCCGGCACC CCCTCAAGTA TCTGATCGTT TGGCGAGCCT CTAGAGGTGG CACCCCCAGG
    GAGGTGGTGG TGGAGGGACC CGCCGCCTCC ACGGAGCTGC ACAACCTGGC CTCCCGCACA
    GAGTACCTGG TCTCCGTGTT CCCCATCTAT GAGGGCGGGG TTGGCGAAGG CCTGCGGGGC
    CTGGTGACCA CAGCACCTCT GCCTCCGCCC CGGGCGCTGA CCCTGGCCGC AGTGACGCCC
    AGAACCGTCC ACCTCACCTG GCAGCCCTCG GCCGGGGCCA CCCACTACCT GGTGCGATGT
    TCTCCTGCTT CCCCCAAGGG TGAAGAGGAG GAGCGAGAGG TGCAGGTCGG GCGGCCCGAG
    GTGCTGCTGG ATGGCCTGGA ACCTGGCAGG GACTATGAGG TCTCGGTGCA GAGCCTGCGA
    GGCCCTGAGG GCAGCGAGGC CCGGGGCATC CGTGCCAGGA CCCCCACCCT GGCCCCCCCG
    AGACACCTGG GCTTCTCAGA CGTGAGCCAC GACGCGGCAC GAGTGTTCTG GGAGGGTGCC
    CCGAGGCCTG TGCGCCTGGT CAGGGTCACC TATGTGTCCA GCGAGGGTGG ACACTCGGGG
    CAGACAGAGG CTCCTGGGAA CGCCACCTCG GCCACGCTGG GGCCTCTCTC TTCCTCCACC
    ACCTACACTG TCCGTGTCAC CTGCCTCTAC CCTGGGGGTG GCTCCTCTAC GCTGACTGGC
    CGGGTGACCA CCAAGAAAGC TCCCAGCCCA AGCCAGCTGT CCATGACGGA GCTGCCAGGG
    GATGCAGTCC AGCTGGCGTG GGTGGCCGCA GCCCCGTCTG GCGTGCTTGT CTACCAGATC
    ACGTGGACGC CCCTGGGAGA GGGGAAGGCT CACGAGATCT CTGTCCCAGG GAACCTCGGC
    ACGGCCGTCC TGCCTGGCCT AGGGAGGCAC ACAGAGTACG ACGTCACCAT CTTGGCCTAC
    TACAGGGACG GGGCCCGCAG TGACCCTGTG TCCCTCCGCT ATACCCCCTC CACGGTGAGC
    AGGAGCCCAC CCTCCAACCT GGCCCTGGCC TCGGAGACCC CCGACAGCCT GCAGGTCAGC
    TGGACGCCCC CGCTTGGCCG CGTGCTCCAT TACTGGCTCA CCTACGCCCC CGCCTCTGGC
    TTGGGACCCG AGAAATCCGT CTCTGTGCCA GGAGCCAGGA GCCACGTGAC ACTGCCCGAC
    CTGCAGGCAG CCACGAAGTA CAGGGTCCTG GTCTCAGCTA TCTATGCAGC AGGCAGGAGT
    GAGGCTGTGT CTGCCACGGG CCAGACAGCC TGCCCAGCCC TCCGCCCTGA CGGCTCCCTC
    CCAGGGTTTG ACCTGATGGT GGCCTTCAGC CTGGTGGAAA AGGCTTATGC GTCCATCCGG
    GGCGTGGCCA TGGAGCCCTC TGCCTTCGGT GGGACCCCGA CCTTCACGCT CTTCAAGGAC
    GCCCAGCTGA CAAGACGGGT CAGTGACGTC TACCCAGCCC CCCTACCTCC AGAGCACACC
    ATCGTCTTCC TTGTGCGCCT ACTTCCCGAG ACACCCCGTG AGGCCTTCGC GCTGTGGCAG
    ATGACAGCCG AGGACTTCCA GCCCCTCCTT GGGGTTCTGC TGGATGCCGG GAAGAAGTCC
    CTGACCTACT TCCACCGTGA CCCCAGGGCT GCCTTGCAGG AGGCCACCTT CGACCCGCAG
    GAAGTGAGGA AGATTTTCTT CGGGAGCTTC CACAAGGTGC ACGTGGCTGT GGGCCGCTCC
    AAGGTCAGGC TCTATGTGGA CTGCCGGAAG GTGGCTGAGC GGCCCCTTGG GGAGATGGGC
    AGCCCACCCG CTGCGGGCTT CGTCACGCTG GGGAGGCTGG CCAAGGCCAG GGGCCCCCGG
    AGCAGTTCGG CCGCGTTTCA GCTCCAGATG CTGCAGATCG TGTGCAGTGA CACCTGGGCC
    GATGAGGACC GGTGCTGTGA GCTCCCTGCC TCGAGGGATG GAGAGACCTG CCCCGCCTTC
    GTGTCTGCCT GTTCCTGTTC CTCAGAGACC CCTGGGCCCC CAGGACCTCA AGGACCCCCA
    GGCCTCCCTG GGAGGAATGG CACCCCAGGA GAGCAGGGCT TCCCAGGGCC CAGGGGTCCA
    CCAGGGGTCA AAGGAGAGAA GGGAGACCAT GGGCTTCCAG GCTTGCAGGG CCACCCCGGC
    CACCAGGGCA TCCCCGGGAG AGTTGGCCTC CAGGGACCAA AGGGAATGAG AGGCCTGGAG
    GGAACTGCTG GCCTGCCTGG ACCCCCTGGC CCCAGGGGGT TCCAGGGCAT GGCAGGGGCC
    AGGGGCACTA GTGGAGAGCG AGGACCTCCA GGGACCGTGG GGCCCACAGG ACTGCCAGGG
    CCCAAAGGGG AACGAGGAGA GAAGGGCGAG CCGCAGTCCC TTGCCACCCT CTACCAGCTT
    GTGAGCCAGG CCTCACACGT GTCAAAGTTC GACTCCTTCC ACGAGAACAC CAGGCCCCCC
    ATGCCCATCT TGGAGCAGAA GCTGGAGCCG GGCACTGAGC CCCTGGGGTC CCCTGGCACC
    CGCAGCAAGG CCCTGGTTCC TGGAGAATGG GGGCGTGGTG GCCGCCACCT TGAGGGCAGA
    GGGGAGCCTG GAGCTGTTGG TCAGATGGGC AGCCCTGGGC AGCAGGGGGC TAGCACCCAG
    GGCCTCTGGG AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70752
    Clone ID Related Accession (Same CDS sequence) XM_011528939.1
    Accession Version XM_011528939.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3996bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-1(XX) chain isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCTCCG GAGACCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
    CTGGGAAGAG AGCAAGTTCA AGCAAGCGGT CTCCTGAGGC TGGCTGTGCT GCCTGAGGAC
    CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG
    AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC
    ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC
    TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC
    CTGGACAGGA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC
    ACGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG
    GATCCGCGCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG
    CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGCAT TGGCCACAGT
    CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGTGTCATCG CACCCTTTGA AATCGGGCCG
    GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG
    AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG
    GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGCG
    GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG
    GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG
    GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC
    ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTCGGCGCGC TGGCTGGCCT GCTCAGCCGT
    CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA
    GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC
    ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA
    GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GACCCGCCGC CTCCACGGAG
    CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC
    GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG
    CTGACCCTGG CCGCAGTGAC GCCCAGAACC GTCCACCTCA CCTGGCAGCC CTCGGCCGGG
    GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAAGA GGAGGAGCGA
    GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT
    GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCGTGCC
    AGGACCCCCA CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG
    GCACGAGTGT TCTGGGAGGG TGCCCCGAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTG
    TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG
    CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG
    GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG
    CTGTCCATGA CGGAGCTGCC AGGGGATGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG
    TCTGGCGTGC TTGTCTACCA GATCACGTGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG
    ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG
    TACGACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC
    CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG
    ACCCCCGACA GCCTGCAGGT CAGCTGGACG CCCCCGCTTG GCCGCGTGCT CCATTACTGG
    CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCCGAGAAAT CCGTCTCTGT GCCAGGAGCC
    AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGGTCTCA
    GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA CGGGCCAGAC AGCAGCCTGC
    CCAGCCCTCC GCCCTGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG
    GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG
    ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCTAC
    CCAGCCCCCC TACCTCCAGA GCACACCATC GTCTTCCTTG TGCGCCTACT TCCCGAGACA
    CCCCGTGAGG CCTTCGCGCT GTGGCAGATG ACAGCCGAGG ACTTCCAGCC CCTCCTTGGG
    GTTCTGCTGG ATGCCGGGAA GAAGTCCCTG ACCTACTTCC ACCGTGACCC CAGGGCTGCC
    TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC
    AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG
    GCTGAGCGGC CCCTTGGGGA GATGGGCAGC CCACCCGCTG CGGGCTTCGT CACGCTGGGG
    AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG CGTTTCAGCT CCAGATGCTG
    CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG
    AGGGATGGAG AGACCTGCCC CGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT
    GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG
    CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT
    CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGTG CAGTCCAGGG GCCTGTGGGT
    CCACCAGGGG TCAAAGGAGA GAAGGGAGAC CATGGGCTTC CAGGCTTGCA GGGCCACCCC
    GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG
    GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG
    GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA
    GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG
    CTTGTGAGCC AGGCCTGTGA GTCTGCCATT CAGACACACG TGTCAAAGTT CGACTCCTTC
    CACGAGAACA CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG
    CCCCTGGGGT CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT
    GGCCGCCACC TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGATGGG CAGCCCTGGG
    CAGCAGGGGG CTAGCACCCA GGGCCTCTGG GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011527241.1
    CDS118..4113
    Translation

    Target ORF information:

    RefSeq Version XM_011528939.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type XX alpha 1 chain (COL20A1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011528939.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGCTCCG GAGACCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
    CTGGGAAGAG AGCAAGTTCA AGCAAGCGGT CTCCTGAGGC TGGCTGTGCT GCCTGAGGAC
    CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG
    AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC
    ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC
    TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC
    CTGGACAGGA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC
    ACGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG
    GATCCGCGCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG
    CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGCAT TGGCCACAGT
    CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGTGTCATCG CACCCTTTGA AATCGGGCCG
    GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG
    AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG
    GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGCG
    GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG
    GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG
    GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC
    ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTCGGCGCGC TGGCTGGCCT GCTCAGCCGT
    CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC AGCGGCTCCA
    GCCCTGGACA CCCTCCCTGC CCCCACCAGC CTGGTCCTGA GCCAGGTGAC CTCCTCCAGC
    ATCCGCCTGT CCTGGACTCC AGCCCCCCGG CACCCCCTCA AGTATCTGAT CGTTTGGCGA
    GCCTCTAGAG GTGGCACCCC CAGGGAGGTG GTGGTGGAGG GACCCGCCGC CTCCACGGAG
    CTGCACAACC TGGCCTCCCG CACAGAGTAC CTGGTCTCCG TGTTCCCCAT CTATGAGGGC
    GGGGTTGGCG AAGGCCTGCG GGGCCTGGTG ACCACAGCAC CTCTGCCTCC GCCCCGGGCG
    CTGACCCTGG CCGCAGTGAC GCCCAGAACC GTCCACCTCA CCTGGCAGCC CTCGGCCGGG
    GCCACCCACT ACCTGGTGCG ATGTTCTCCT GCTTCCCCCA AGGGTGAAGA GGAGGAGCGA
    GAGGTGCAGG TCGGGCGGCC CGAGGTGCTG CTGGATGGCC TGGAACCTGG CAGGGACTAT
    GAGGTCTCGG TGCAGAGCCT GCGAGGCCCT GAGGGCAGCG AGGCCCGGGG CATCCGTGCC
    AGGACCCCCA CCCTGGCCCC CCCGAGACAC CTGGGCTTCT CAGACGTGAG CCACGACGCG
    GCACGAGTGT TCTGGGAGGG TGCCCCGAGG CCTGTGCGCC TGGTCAGGGT CACCTATGTG
    TCCAGCGAGG GTGGACACTC GGGGCAGACA GAGGCTCCTG GGAACGCCAC CTCGGCCACG
    CTGGGGCCTC TCTCTTCCTC CACCACCTAC ACTGTCCGTG TCACCTGCCT CTACCCTGGG
    GGTGGCTCCT CTACGCTGAC TGGCCGGGTG ACCACCAAGA AAGCTCCCAG CCCAAGCCAG
    CTGTCCATGA CGGAGCTGCC AGGGGATGCA GTCCAGCTGG CGTGGGTGGC CGCAGCCCCG
    TCTGGCGTGC TTGTCTACCA GATCACGTGG ACGCCCCTGG GAGAGGGGAA GGCTCACGAG
    ATCTCTGTCC CAGGGAACCT CGGCACGGCC GTCCTGCCTG GCCTAGGGAG GCACACAGAG
    TACGACGTCA CCATCTTGGC CTACTACAGG GACGGGGCCC GCAGTGACCC TGTGTCCCTC
    CGCTATACCC CCTCCACGGT GAGCAGGAGC CCACCCTCCA ACCTGGCCCT GGCCTCGGAG
    ACCCCCGACA GCCTGCAGGT CAGCTGGACG CCCCCGCTTG GCCGCGTGCT CCATTACTGG
    CTCACCTACG CCCCCGCCTC TGGCTTGGGA CCCGAGAAAT CCGTCTCTGT GCCAGGAGCC
    AGGAGCCACG TGACACTGCC CGACCTGCAG GCAGCCACGA AGTACAGGGT CCTGGTCTCA
    GCTATCTATG CAGCAGGCAG GAGTGAGGCT GTGTCTGCCA CGGGCCAGAC AGCAGCCTGC
    CCAGCCCTCC GCCCTGACGG CTCCCTCCCA GGGTTTGACC TGATGGTGGC CTTCAGCCTG
    GTGGAAAAGG CTTATGCGTC CATCCGGGGC GTGGCCATGG AGCCCTCTGC CTTCGGTGGG
    ACCCCGACCT TCACGCTCTT CAAGGACGCC CAGCTGACAA GACGGGTCAG TGACGTCTAC
    CCAGCCCCCC TACCTCCAGA GCACACCATC GTCTTCCTTG TGCGCCTACT TCCCGAGACA
    CCCCGTGAGG CCTTCGCGCT GTGGCAGATG ACAGCCGAGG ACTTCCAGCC CCTCCTTGGG
    GTTCTGCTGG ATGCCGGGAA GAAGTCCCTG ACCTACTTCC ACCGTGACCC CAGGGCTGCC
    TTGCAGGAGG CCACCTTCGA CCCGCAGGAA GTGAGGAAGA TTTTCTTCGG GAGCTTCCAC
    AAGGTGCACG TGGCTGTGGG CCGCTCCAAG GTCAGGCTCT ATGTGGACTG CCGGAAGGTG
    GCTGAGCGGC CCCTTGGGGA GATGGGCAGC CCACCCGCTG CGGGCTTCGT CACGCTGGGG
    AGGCTGGCCA AGGCCAGGGG CCCCCGGAGC AGTTCGGCCG CGTTTCAGCT CCAGATGCTG
    CAGATCGTGT GCAGTGACAC CTGGGCCGAT GAGGACCGGT GCTGTGAGCT CCCTGCCTCG
    AGGGATGGAG AGACCTGCCC CGCCTTCGTG TCTGCCTGTT CCTGTTCCTC AGAGACCCCT
    GGGCCCCCAG GACCTCAAGG ACCCCCAGGC CTCCCTGGGA GGAATGGCAC CCCAGGAGAG
    CAGGGCTTCC CAGGGCCCAG GGGAGAGCCC GGGCCACCCG GACAGATGGG ACCAGAAGGT
    CCTGGAGGCC AGCAGGGCTC GCCGGGGACC CAGGGCCGTG CAGTCCAGGG GCCTGTGGGT
    CCACCAGGGG TCAAAGGAGA GAAGGGAGAC CATGGGCTTC CAGGCTTGCA GGGCCACCCC
    GGCCACCAGG GCATCCCCGG GAGAGTTGGC CTCCAGGGAC CAAAGGGAAT GAGAGGCCTG
    GAGGGAACTG CTGGCCTGCC TGGACCCCCT GGCCCCAGGG GGTTCCAGGG CATGGCAGGG
    GCCAGGGGCA CTAGTGGAGA GCGAGGACCT CCAGGGACCG TGGGGCCCAC AGGACTGCCA
    GGGCCCAAAG GGGAACGAGG AGAGAAGGGC GAGCCGCAGT CCCTTGCCAC CCTCTACCAG
    CTTGTGAGCC AGGCCTGTGA GTCTGCCATT CAGACACACG TGTCAAAGTT CGACTCCTTC
    CACGAGAACA CCAGGCCCCC CATGCCCATC TTGGAGCAGA AGCTGGAGCC GGGCACTGAG
    CCCCTGGGGT CCCCTGGCAC CCGCAGCAAG GCCCTGGTTC CTGGAGAATG GGGGCGTGGT
    GGCCGCCACC TTGAGGGCAG AGGGGAGCCT GGAGCTGTTG GTCAGATGGG CAGCCCTGGG
    CAGCAGGGGG CTAGCACCCA GGGCCTCTGG GAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70753
    Clone ID Related Accession (Same CDS sequence) XM_011528940.1
    Accession Version XM_011528940.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-1(XX) chain isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCTCCG GAGACCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
    CTGGGAAGAG AGCAAGTTCA AGCAAGCGGT CTCCTGAGGC TGGCTGTGCT GCCTGAGGAC
    CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG
    AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC
    ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC
    TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC
    CTGGACAGGA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC
    ACGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG
    GATCCGCGCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG
    CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGCAT TGGCCACAGT
    CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGTGTCATCG CACCCTTTGA AATCGGGCCG
    GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG
    AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG
    GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGCG
    GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG
    GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG
    GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC
    ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTCGGCGCGC TGGCTGGCCT GCTCAGCCGT
    CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC GGCTCCAGCC
    CTGGACACCC TCCCTGCCCC CACCAGCCTG GTCCTGAGCC AGGTGACCTC CTCCAGCATC
    CGCCTGTCCT GGACTCCAGC CCCCCGGCAC CCCCTCAAGT ATCTGATCGT TTGGCGAGCC
    TCTAGAGGTG GCACCCCCAG GGAGGTGGTG GTGGAGGGAC CCGCCGCCTC CACGGAGCTG
    CACAACCTGG CCTCCCGCAC AGAGTACCTG GTCTCCGTGT TCCCCATCTA TGAGGGCGGG
    GTTGGCGAAG GCCTGCGGGG CCTGGTGACC ACAGCACCTC TGCCTCCGCC CCGGGCGCTG
    ACCCTGGCCG CAGTGACGCC CAGAACCGTC CACCTCACCT GGCAGCCCTC GGCCGGGGCC
    ACCCACTACC TGGTGCGATG TTCTCCTGCT TCCCCCAAGG GTGAAGAGGA GGAGCGAGAG
    GTGCAGGTCG GGCGGCCCGA GGTGCTGCTG GATGGCCTGG AACCTGGCAG GGACTATGAG
    GTCTCGGTGC AGAGCCTGCG AGGCCCTGAG GGCAGCGAGG CCCGGGGCAT CCGTGCCAGG
    ACCCCCACCC TGGCCCCCCC GAGACACCTG GGCTTCTCAG ACGTGAGCCA CGACGCGGCA
    CGAGTGTTCT GGGAGGGTGC CCCGAGGCCT GTGCGCCTGG TCAGGGTCAC CTATGTGTCC
    AGCGAGGGTG GACACTCGGG GCAGACAGAG GCTCCTGGGA ACGCCACCTC GGCCACGCTG
    GGGCCTCTCT CTTCCTCCAC CACCTACACT GTCCGTGTCA CCTGCCTCTA CCCTGGGGGT
    GGCTCCTCTA CGCTGACTGG CCGGGTGACC ACCAAGAAAG CTCCCAGCCC AAGCCAGCTG
    TCCATGACGG AGCTGCCAGG GGATGCAGTC CAGCTGGCGT GGGTGGCCGC AGCCCCGTCT
    GGCGTGCTTG TCTACCAGAT CACGTGGACG CCCCTGGGAG AGGGGAAGGC TCACGAGATC
    TCTGTCCCAG GGAACCTCGG CACGGCCGTC CTGCCTGGCC TAGGGAGGCA CACAGAGTAC
    GACGTCACCA TCTTGGCCTA CTACAGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGC
    TATACCCCCT CCACGGTGAG CAGGAGCCCA CCCTCCAACC TGGCCCTGGC CTCGGAGACC
    CCCGACAGCC TGCAGGTCAG CTGGACGCCC CCGCTTGGCC GCGTGCTCCA TTACTGGCTC
    ACCTACGCCC CCGCCTCTGG CTTGGGACCC GAGAAATCCG TCTCTGTGCC AGGAGCCAGG
    AGCCACGTGA CACTGCCCGA CCTGCAGGCA GCCACGAAGT ACAGGGTCCT GGTCTCAGCT
    ATCTATGCAG CAGGCAGGAG TGAGGCTGTG TCTGCCACGG GCCAGACAGC CTGCCCAGCC
    CTCCGCCCTG ACGGCTCCCT CCCAGGGTTT GACCTGATGG TGGCCTTCAG CCTGGTGGAA
    AAGGCTTATG CGTCCATCCG GGGCGTGGCC ATGGAGCCCT CTGCCTTCGG TGGGACCCCG
    ACCTTCACGC TCTTCAAGGA CGCCCAGCTG ACAAGACGGG TCAGTGACGT CTACCCAGCC
    CCCCTACCTC CAGAGCACAC CATCGTCTTC CTTGTGCGCC TACTTCCCGA GACACCCCGT
    GAGGCCTTCG CGCTGTGGCA GATGACAGCC GAGGACTTCC AGCCCCTCCT TGGGGTTCTG
    CTGGATGCCG GGAAGAAGTC CCTGACCTAC TTCCACCGTG ACCCCAGGGC TGCCTTGCAG
    GAGGCCACCT TCGACCCGCA GGAAGTGAGG AAGATTTTCT TCGGGAGCTT CCACAAGGTG
    CACGTGGCTG TGGGCCGCTC CAAGGTCAGG CTCTATGTGG ACTGCCGGAA GGTGGCTGAG
    CGGCCCCTTG GGGAGATGGG CAGCCCACCC GCTGCGGGCT TCGTCACGCT GGGGAGGCTG
    GCCAAGGCCA GGGGCCCCCG GAGCAGTTCG GCCGCGTTTC AGCTCCAGAT GCTGCAGATC
    GTGTGCAGTG ACACCTGGGC CGATGAGGAC CGGTGCTGTG AGCTCCCTGC CTCGAGGGAT
    GGAGAGACCT GCCCCGCCTT CGTGTCTGCC TGTTCCTGTT CCTCAGAGAC CCCTGGGCCC
    CCAGGACCTC AAGGACCCCC AGGCCTCCCT GGGAGGAATG GCACCCCAGG AGAGCAGGGC
    TTCCCAGGGC CCAGGGGTCC ACCAGGGGTC AAAGGAGAGA AGGGAGACCA TGGGCTTCCA
    GGCTTGCAGG GCCACCCCGG CCACCAGGGC ATCCCCGGGA GAGTTGGCCT CCAGGGACCA
    AAGGGAATGA GAGGCCTGGA GGGAACTGCT GGCCTGCCTG GACCCCCTGG CCCCAGGGGG
    TTCCAGGGCA TGGCAGGGGC CAGGGGCACT AGTGGAGAGC GAGGACCTCC AGGGACCGTG
    GGGCCCACAG GACTGCCAGG GCCCAAAGGG GAACGAGGAG AGAAGGGCGA GCCGCAGTCC
    CTTGCCACCC TCTACCAGCT TGTGAGCCAG GCCTGTGAGT CTGCCATTCA GACACACGTG
    TCAAAGTTCG ACTCCTTCCA CGAGAACACC AGGCCCCCCA TGCCCATCTT GGAGCAGAAG
    CTGGAGCCGG GCACTGAGCC CCTGGGGTCC CCTGGCACCC GCAGCAAGGC CCTGGTTCCT
    GGAGAATGGG GGCGTGGTGG CCGCCACCTT GAGGGCAGAG GGGAGCCTGG AGCTGTTGGT
    CAGATGGGCA GCCCTGGGCA GCAGGGGGCT AGCACCCAGG GCCTCTGGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011527242.1
    CDS118..4011
    Translation

    Target ORF information:

    RefSeq Version XM_011528940.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type XX alpha 1 chain (COL20A1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011528940.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGAGCTCCG GAGACCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
    CTGGGAAGAG AGCAAGTTCA AGCAAGCGGT CTCCTGAGGC TGGCTGTGCT GCCTGAGGAC
    CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG
    AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC
    ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC
    TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC
    CTGGACAGGA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC
    ACGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG
    GATCCGCGCA CTCCTGGTGG GTCAGAGTGG AGAGAGACCG GCCCCCAGTT CCGCTGCCTG
    CCCCCCGTGC CTGCTGACAT GGTCTTCCTG GTGGACGGGT CCTGGAGCAT TGGCCACAGT
    CACTTCCAGC AGGTCAAGGA CTTCCTGGCC AGTGTCATCG CACCCTTTGA AATCGGGCCG
    GATAAGGTCC AAGTAGGCCT GACTCAGTAC AGCGGGGATG CTCAGACTGA GTGGGACCTG
    AACTCCCTCA GCACCAAGGA ACAGGTGCTG GCAGCTGTGC GCCGCCTCCG CTACAAGGGG
    GGGAACACGT TCACAGGCCT TGCCCTGACC CACGTGCTGG GGCAGAACCT GCAGCCGGCG
    GCTGGCCTCC GTCCAGAGGC AGCCAAGGTG GTGATTCTGG TGACGGACGG CAAGTCCCAG
    GACGATGTGC ACACTGCTGC CCGTGTCCTC AAGGACCTGG GCGTGAACGT CTTCGCTGTG
    GGTGTGAAGA ACGCCGATGA GGCTGAGCTG AGGCTCCTGG CGTCCCCGCC GAGGGACATC
    ACCGTCCACA GCGTGCTGGA CTTCCTGCAG CTCGGCGCGC TGGCTGGCCT GCTCAGCCGT
    CTCATCTGCC AGAGGCTCCA GGGTGGGAGC CCGCGGCAGG GCCCAGCAGC GGCTCCAGCC
    CTGGACACCC TCCCTGCCCC CACCAGCCTG GTCCTGAGCC AGGTGACCTC CTCCAGCATC
    CGCCTGTCCT GGACTCCAGC CCCCCGGCAC CCCCTCAAGT ATCTGATCGT TTGGCGAGCC
    TCTAGAGGTG GCACCCCCAG GGAGGTGGTG GTGGAGGGAC CCGCCGCCTC CACGGAGCTG
    CACAACCTGG CCTCCCGCAC AGAGTACCTG GTCTCCGTGT TCCCCATCTA TGAGGGCGGG
    GTTGGCGAAG GCCTGCGGGG CCTGGTGACC ACAGCACCTC TGCCTCCGCC CCGGGCGCTG
    ACCCTGGCCG CAGTGACGCC CAGAACCGTC CACCTCACCT GGCAGCCCTC GGCCGGGGCC
    ACCCACTACC TGGTGCGATG TTCTCCTGCT TCCCCCAAGG GTGAAGAGGA GGAGCGAGAG
    GTGCAGGTCG GGCGGCCCGA GGTGCTGCTG GATGGCCTGG AACCTGGCAG GGACTATGAG
    GTCTCGGTGC AGAGCCTGCG AGGCCCTGAG GGCAGCGAGG CCCGGGGCAT CCGTGCCAGG
    ACCCCCACCC TGGCCCCCCC GAGACACCTG GGCTTCTCAG ACGTGAGCCA CGACGCGGCA
    CGAGTGTTCT GGGAGGGTGC CCCGAGGCCT GTGCGCCTGG TCAGGGTCAC CTATGTGTCC
    AGCGAGGGTG GACACTCGGG GCAGACAGAG GCTCCTGGGA ACGCCACCTC GGCCACGCTG
    GGGCCTCTCT CTTCCTCCAC CACCTACACT GTCCGTGTCA CCTGCCTCTA CCCTGGGGGT
    GGCTCCTCTA CGCTGACTGG CCGGGTGACC ACCAAGAAAG CTCCCAGCCC AAGCCAGCTG
    TCCATGACGG AGCTGCCAGG GGATGCAGTC CAGCTGGCGT GGGTGGCCGC AGCCCCGTCT
    GGCGTGCTTG TCTACCAGAT CACGTGGACG CCCCTGGGAG AGGGGAAGGC TCACGAGATC
    TCTGTCCCAG GGAACCTCGG CACGGCCGTC CTGCCTGGCC TAGGGAGGCA CACAGAGTAC
    GACGTCACCA TCTTGGCCTA CTACAGGGAC GGGGCCCGCA GTGACCCTGT GTCCCTCCGC
    TATACCCCCT CCACGGTGAG CAGGAGCCCA CCCTCCAACC TGGCCCTGGC CTCGGAGACC
    CCCGACAGCC TGCAGGTCAG CTGGACGCCC CCGCTTGGCC GCGTGCTCCA TTACTGGCTC
    ACCTACGCCC CCGCCTCTGG CTTGGGACCC GAGAAATCCG TCTCTGTGCC AGGAGCCAGG
    AGCCACGTGA CACTGCCCGA CCTGCAGGCA GCCACGAAGT ACAGGGTCCT GGTCTCAGCT
    ATCTATGCAG CAGGCAGGAG TGAGGCTGTG TCTGCCACGG GCCAGACAGC CTGCCCAGCC
    CTCCGCCCTG ACGGCTCCCT CCCAGGGTTT GACCTGATGG TGGCCTTCAG CCTGGTGGAA
    AAGGCTTATG CGTCCATCCG GGGCGTGGCC ATGGAGCCCT CTGCCTTCGG TGGGACCCCG
    ACCTTCACGC TCTTCAAGGA CGCCCAGCTG ACAAGACGGG TCAGTGACGT CTACCCAGCC
    CCCCTACCTC CAGAGCACAC CATCGTCTTC CTTGTGCGCC TACTTCCCGA GACACCCCGT
    GAGGCCTTCG CGCTGTGGCA GATGACAGCC GAGGACTTCC AGCCCCTCCT TGGGGTTCTG
    CTGGATGCCG GGAAGAAGTC CCTGACCTAC TTCCACCGTG ACCCCAGGGC TGCCTTGCAG
    GAGGCCACCT TCGACCCGCA GGAAGTGAGG AAGATTTTCT TCGGGAGCTT CCACAAGGTG
    CACGTGGCTG TGGGCCGCTC CAAGGTCAGG CTCTATGTGG ACTGCCGGAA GGTGGCTGAG
    CGGCCCCTTG GGGAGATGGG CAGCCCACCC GCTGCGGGCT TCGTCACGCT GGGGAGGCTG
    GCCAAGGCCA GGGGCCCCCG GAGCAGTTCG GCCGCGTTTC AGCTCCAGAT GCTGCAGATC
    GTGTGCAGTG ACACCTGGGC CGATGAGGAC CGGTGCTGTG AGCTCCCTGC CTCGAGGGAT
    GGAGAGACCT GCCCCGCCTT CGTGTCTGCC TGTTCCTGTT CCTCAGAGAC CCCTGGGCCC
    CCAGGACCTC AAGGACCCCC AGGCCTCCCT GGGAGGAATG GCACCCCAGG AGAGCAGGGC
    TTCCCAGGGC CCAGGGGTCC ACCAGGGGTC AAAGGAGAGA AGGGAGACCA TGGGCTTCCA
    GGCTTGCAGG GCCACCCCGG CCACCAGGGC ATCCCCGGGA GAGTTGGCCT CCAGGGACCA
    AAGGGAATGA GAGGCCTGGA GGGAACTGCT GGCCTGCCTG GACCCCCTGG CCCCAGGGGG
    TTCCAGGGCA TGGCAGGGGC CAGGGGCACT AGTGGAGAGC GAGGACCTCC AGGGACCGTG
    GGGCCCACAG GACTGCCAGG GCCCAAAGGG GAACGAGGAG AGAAGGGCGA GCCGCAGTCC
    CTTGCCACCC TCTACCAGCT TGTGAGCCAG GCCTGTGAGT CTGCCATTCA GACACACGTG
    TCAAAGTTCG ACTCCTTCCA CGAGAACACC AGGCCCCCCA TGCCCATCTT GGAGCAGAAG
    CTGGAGCCGG GCACTGAGCC CCTGGGGTCC CCTGGCACCC GCAGCAAGGC CCTGGTTCCT
    GGAGAATGGG GGCGTGGTGG CCGCCACCTT GAGGGCAGAG GGGAGCCTGG AGCTGTTGGT
    CAGATGGGCA GCCCTGGGCA GCAGGGGGCT AGCACCCAGG GCCTCTGGGA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70750
    Clone ID Related Accession (Same CDS sequence) XM_011528937.1
    Accession Version XM_011528937.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4290bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-1(XX) chain isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCTCCG GAGACCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
    CTGGGAAGAG AGCAAGTTCA AGCAAGCGGT CTCCTGAGGC TGGCTGTGCT GCCTGAGGAC
    CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG
    AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC
    ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC
    TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC
    CTGGACAGGA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC
    ACGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG
    GATCCGCGCA CTCCTGCCGG CCCCCAGTTC CGCTGCCTGC CCCCCGTGCC TGCTGACATG
    GTCTTCCTGG TGGACGGGTC CTGGAGCATT GGCCACAGTC ACTTCCAGCA GGTCAAGGAC
    TTCCTGGCCA GTGTCATCGC ACCCTTTGAA ATCGGGCCGG ATAAGGTCCA AGTAGGCCTG
    ACTCAGTACA GCGGGGATGC TCAGACTGAG TGGGACCTGA ACTCCCTCAG CACCAAGGAA
    CAGGTGCTGG CAGCTGTGCG CCGCCTCCGC TACAAGGGGG GGAACACGTT CACAGGCCTT
    GCCCTGACCC ACGTGCTGGG GCAGAACCTG CAGCCGGCGG CTGGCCTCCG TCCAGAGGCA
    GCCAAGGTGG TGATTCTGGT GACGGACGGC AAGTCCCAGG ACGATGTGCA CACTGCTGCC
    CGTGTCCTCA AGGACCTGGG CGTGAACGTC TTCGCTGTGG GTGTGAAGAA CGCCGATGAG
    GCTGAGCTGA GGCTCCTGGC GTCCCCGCCG AGGGACATCA CCGTCCACAG CGTGCTGGAC
    TTCCTGCAGC TCGGCGCGCT GGCTGGCCTG CTCAGCCGTC TCATCTGCCA GAGGCTCCAG
    GGTGGGAGCC CGCGGCAGGG CCCAGCAGCA GCGGCTCCAG CCCTGGACAC CCTCCCTGCC
    CCCACCAGCC TGGTCCTGAG CCAGGTGACC TCCTCCAGCA TCCGCCTGTC CTGGACTCCA
    GCCCCCCGGC ACCCCCTCAA GTATCTGATC GTTTGGCGAG CCTCTAGAGG TGGCACCCCC
    AGGGAGGTGG TGGTGGAGGG ACCCGCCGCC TCCACGGAGC TGCACAACCT GGCCTCCCGC
    ACAGAGTACC TGGTCTCCGT GTTCCCCATC TATGAGGGCG GGGTTGGCGA AGGCCTGCGG
    GGCCTGGTGA CCACAGCACC TCTGCCTCCG CCCCGGGCGC TGACCCTGGC CGCAGTGACG
    CCCAGAACCG TCCACCTCAC CTGGCAGCCC TCGGCCGGGG CCACCCACTA CCTGGTGCGA
    TGTTCTCCTG CTTCCCCCAA GGGTGAAGAG GAGGAGCGAG AGGTGCAGGT CGGGCGGCCC
    GAGGTGCTGC TGGATGGCCT GGAACCTGGC AGGGACTATG AGGTCTCGGT GCAGAGCCTG
    CGAGGCCCTG AGGGCAGCGA GGCCCGGGGC ATCCGTGCCA GGACCCCCAC CCTGGCCCCC
    CCGAGACACC TGGGCTTCTC AGACGTGAGC CACGACGCGG CACGAGTGTT CTGGGAGGGT
    GCCCCGAGGC CTGTGCGCCT GGTCAGGGTC ACCTATGTGT CCAGCGAGGG TGGACACTCG
    GGGCAGACAG AGGCTCCTGG GAACGCCACC TCGGCCACGC TGGGGCCTCT CTCTTCCTCC
    ACCACCTACA CTGTCCGTGT CACCTGCCTC TACCCTGGGG GTGGCTCCTC TACGCTGACT
    GGCCGGGTGA CCACCAAGAA AGCTCCCAGC CCAAGCCAGC TGTCCATGAC GGAGCTGCCA
    GGGGATGCAG TCCAGCTGGC GTGGGTGGCC GCAGCCCCGT CTGGCGTGCT TGTCTACCAG
    ATCACGTGGA CGCCCCTGGG AGAGGGGAAG GCTCACGAGA TCTCTGTCCC AGGGAACCTC
    GGCACGGCCG TCCTGCCTGG CCTAGGGAGG CACACAGAGT ACGACGTCAC CATCTTGGCC
    TACTACAGGG ACGGGGCCCG CAGTGACCCT GTGTCCCTCC GCTATACCCC CTCCACGGTG
    AGCAGGAGCC CACCCTCCAA CCTGGCCCTG GCCTCGGAGA CCCCCGACAG CCTGCAGGTC
    AGCTGGACGC CCCCGCTTGG CCGCGTGCTC CATTACTGGC TCACCTACGC CCCCGCCTCT
    GGCTTGGGAC CCGAGAAATC CGTCTCTGTG CCAGGAGCCA GGAGCCACGT GACACTGCCC
    GACCTGCAGG CAGCCACGAA GTACAGGGTC CTGGTCTCAG CTATCTATGC AGCAGGCAGG
    AGTGAGGCTG TGTCTGCCAC GGGCCAGACA GCAGCCTGCC CAGCCCTCCG CCCTGACGGC
    TCCCTCCCAG GGTTTGACCT GATGGTGGCC TTCAGCCTGG TGGAAAAGGC TTATGCGTCC
    ATCCGGGGCG TGGCCATGGA GCCCTCTGCC TTCGGTGGGA CCCCGACCTT CACGCTCTTC
    AAGGACGCCC AGCTGACAAG ACGGGTCAGT GACGTCTACC CAGCCCCCCT ACCTCCAGAG
    CACACCATCG TCTTCCTTGT GCGCCTACTT CCCGAGACAC CCCGTGAGGC CTTCGCGCTG
    TGGCAGATGA CAGCCGAGGA CTTCCAGCCC CTCCTTGGGG TTCTGCTGGA TGCCGGGAAG
    AAGTCCCTGA CCTACTTCCA CCGTGACCCC AGGGCTGCCT TGCAGGAGGC CACCTTCGAC
    CCGCAGGAAG TGAGGAAGAT TTTCTTCGGG AGCTTCCACA AGGTGCACGT GGCTGTGGGC
    CGCTCCAAGG TCAGGCTCTA TGTGGACTGC CGGAAGGTGG CTGAGCGGCC CCTTGGGGAG
    ATGGGCAGCC CACCCGCTGC GGGCTTCGTC ACGCTGGGGA GGCTGGCCAA GGCCAGGGGC
    CCCCGGAGCA GTTCGGCCGC GTTTCAGCTC CAGATGCTGC AGATCGTGTG CAGTGACACC
    TGGGCCGATG AGGACCGGTG CTGTGAGCTC CCTGCCTCGA GGGATGGAGA GACCTGCCCC
    GCCTTCGTGT CTGCCTGTTC CTGTTCCTCA GAGACCCCTG GGCCCCCAGG ACCTCAAGGA
    CCCCCAGGCC TCCCTGGGAG GAATGGCACC CCAGGAGAGC AGGGCTTCCC AGGGCCCAGG
    GGAGAGCCCG GGCCACCCGG ACAGATGGGA CCAGAAGGTC CTGGAGGCCA GCAGGGCTCG
    CCGGGGACCC AGGGCCGTGC AGTCCAGGGG CCTGTGGGTC CACCAGGGGT CAAAGGAGAG
    AAGGGAGACC ATGGGCTTCC AGGCTTGCAG GGCCACCCCG GCCACCAGGG CATCCCCGGG
    AGAGTTGGCC TCCAGGGACC AAAGGGAATG AGAGGCCTGG AGGGAACTGC TGGCCTGCCT
    GGACCCCCTG GCCCCAGGGG GTTCCAGGGC ATGGCAGGGG CCAGGGGCAC TAGTGGAGAG
    CGAGGACCTC CAGGGACCGT GGGGCCCACA GGACTGCCAG GGCCCAAAGG GGAACGAGGA
    GAGAAGCACA CGTGTCAAAG TTCGACTCCT TCCACGAGAA CACCAGGCCC CCCATGCCCA
    TCTTGGAGCA GAAGCTGGAG CCGGGCACTG AGCCCCTGGG GTCCCCTGGC ACCCGCAGCA
    AGGCCCTGGT TCCTGGAGAA TGGGGGCGTG GTGGCCGCCA CCTTGAGGGC AGAGGGGAGC
    CTGGAGCTGT TGGTCAGATG GGCAGCCCTG GGCAGCAGGG GGCTAGCACC CAGGGCCTCT
    GGGAGTGACA GGACATTTTC TGCACTGCCC CGAGGAACGC TGAGCCTTCC TCCCTGGGTT
    TGTCTGGACA CCGAGAGCGA CCACATCCTG GAGAAGCCAG GAGAAAAGCT CAGGAAGAGC
    CTGCAGGTGG AAGGAGAGGG AAGCAGCGGC CTCGGCCAAG GCCCACCCCA TACTCTTGGC
    TCTGTAGCAT TTCCAAGTTC AGATAAACCC CTGAGTGCTC ACCCAGCCAA GCGAGGCGGG
    AGTCATTTCC TGGGAGAGTG TCCCCCACCC TGGCTCTGTG CACCCCACCT TGAGGCCCTG
    CAGCTTCAGC CGCAGTGGGG AAGGGGAGGG GCTGGGCTGA TGGCGAATCG GGTTCAGGAG
    GGCAGCAGTG CCCTGTGGCC GTCTCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011527239.1
    CDS118..4407
    Translation

    Target ORF information:

    RefSeq Version XM_011528937.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type XX alpha 1 chain (COL20A1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011528937.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGAGCTCCG GAGACCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
    CTGGGAAGAG AGCAAGTTCA AGCAAGCGGT CTCCTGAGGC TGGCTGTGCT GCCTGAGGAC
    CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG
    AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC
    ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC
    TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC
    CTGGACAGGA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC
    ACGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG
    GATCCGCGCA CTCCTGCCGG CCCCCAGTTC CGCTGCCTGC CCCCCGTGCC TGCTGACATG
    GTCTTCCTGG TGGACGGGTC CTGGAGCATT GGCCACAGTC ACTTCCAGCA GGTCAAGGAC
    TTCCTGGCCA GTGTCATCGC ACCCTTTGAA ATCGGGCCGG ATAAGGTCCA AGTAGGCCTG
    ACTCAGTACA GCGGGGATGC TCAGACTGAG TGGGACCTGA ACTCCCTCAG CACCAAGGAA
    CAGGTGCTGG CAGCTGTGCG CCGCCTCCGC TACAAGGGGG GGAACACGTT CACAGGCCTT
    GCCCTGACCC ACGTGCTGGG GCAGAACCTG CAGCCGGCGG CTGGCCTCCG TCCAGAGGCA
    GCCAAGGTGG TGATTCTGGT GACGGACGGC AAGTCCCAGG ACGATGTGCA CACTGCTGCC
    CGTGTCCTCA AGGACCTGGG CGTGAACGTC TTCGCTGTGG GTGTGAAGAA CGCCGATGAG
    GCTGAGCTGA GGCTCCTGGC GTCCCCGCCG AGGGACATCA CCGTCCACAG CGTGCTGGAC
    TTCCTGCAGC TCGGCGCGCT GGCTGGCCTG CTCAGCCGTC TCATCTGCCA GAGGCTCCAG
    GGTGGGAGCC CGCGGCAGGG CCCAGCAGCA GCGGCTCCAG CCCTGGACAC CCTCCCTGCC
    CCCACCAGCC TGGTCCTGAG CCAGGTGACC TCCTCCAGCA TCCGCCTGTC CTGGACTCCA
    GCCCCCCGGC ACCCCCTCAA GTATCTGATC GTTTGGCGAG CCTCTAGAGG TGGCACCCCC
    AGGGAGGTGG TGGTGGAGGG ACCCGCCGCC TCCACGGAGC TGCACAACCT GGCCTCCCGC
    ACAGAGTACC TGGTCTCCGT GTTCCCCATC TATGAGGGCG GGGTTGGCGA AGGCCTGCGG
    GGCCTGGTGA CCACAGCACC TCTGCCTCCG CCCCGGGCGC TGACCCTGGC CGCAGTGACG
    CCCAGAACCG TCCACCTCAC CTGGCAGCCC TCGGCCGGGG CCACCCACTA CCTGGTGCGA
    TGTTCTCCTG CTTCCCCCAA GGGTGAAGAG GAGGAGCGAG AGGTGCAGGT CGGGCGGCCC
    GAGGTGCTGC TGGATGGCCT GGAACCTGGC AGGGACTATG AGGTCTCGGT GCAGAGCCTG
    CGAGGCCCTG AGGGCAGCGA GGCCCGGGGC ATCCGTGCCA GGACCCCCAC CCTGGCCCCC
    CCGAGACACC TGGGCTTCTC AGACGTGAGC CACGACGCGG CACGAGTGTT CTGGGAGGGT
    GCCCCGAGGC CTGTGCGCCT GGTCAGGGTC ACCTATGTGT CCAGCGAGGG TGGACACTCG
    GGGCAGACAG AGGCTCCTGG GAACGCCACC TCGGCCACGC TGGGGCCTCT CTCTTCCTCC
    ACCACCTACA CTGTCCGTGT CACCTGCCTC TACCCTGGGG GTGGCTCCTC TACGCTGACT
    GGCCGGGTGA CCACCAAGAA AGCTCCCAGC CCAAGCCAGC TGTCCATGAC GGAGCTGCCA
    GGGGATGCAG TCCAGCTGGC GTGGGTGGCC GCAGCCCCGT CTGGCGTGCT TGTCTACCAG
    ATCACGTGGA CGCCCCTGGG AGAGGGGAAG GCTCACGAGA TCTCTGTCCC AGGGAACCTC
    GGCACGGCCG TCCTGCCTGG CCTAGGGAGG CACACAGAGT ACGACGTCAC CATCTTGGCC
    TACTACAGGG ACGGGGCCCG CAGTGACCCT GTGTCCCTCC GCTATACCCC CTCCACGGTG
    AGCAGGAGCC CACCCTCCAA CCTGGCCCTG GCCTCGGAGA CCCCCGACAG CCTGCAGGTC
    AGCTGGACGC CCCCGCTTGG CCGCGTGCTC CATTACTGGC TCACCTACGC CCCCGCCTCT
    GGCTTGGGAC CCGAGAAATC CGTCTCTGTG CCAGGAGCCA GGAGCCACGT GACACTGCCC
    GACCTGCAGG CAGCCACGAA GTACAGGGTC CTGGTCTCAG CTATCTATGC AGCAGGCAGG
    AGTGAGGCTG TGTCTGCCAC GGGCCAGACA GCAGCCTGCC CAGCCCTCCG CCCTGACGGC
    TCCCTCCCAG GGTTTGACCT GATGGTGGCC TTCAGCCTGG TGGAAAAGGC TTATGCGTCC
    ATCCGGGGCG TGGCCATGGA GCCCTCTGCC TTCGGTGGGA CCCCGACCTT CACGCTCTTC
    AAGGACGCCC AGCTGACAAG ACGGGTCAGT GACGTCTACC CAGCCCCCCT ACCTCCAGAG
    CACACCATCG TCTTCCTTGT GCGCCTACTT CCCGAGACAC CCCGTGAGGC CTTCGCGCTG
    TGGCAGATGA CAGCCGAGGA CTTCCAGCCC CTCCTTGGGG TTCTGCTGGA TGCCGGGAAG
    AAGTCCCTGA CCTACTTCCA CCGTGACCCC AGGGCTGCCT TGCAGGAGGC CACCTTCGAC
    CCGCAGGAAG TGAGGAAGAT TTTCTTCGGG AGCTTCCACA AGGTGCACGT GGCTGTGGGC
    CGCTCCAAGG TCAGGCTCTA TGTGGACTGC CGGAAGGTGG CTGAGCGGCC CCTTGGGGAG
    ATGGGCAGCC CACCCGCTGC GGGCTTCGTC ACGCTGGGGA GGCTGGCCAA GGCCAGGGGC
    CCCCGGAGCA GTTCGGCCGC GTTTCAGCTC CAGATGCTGC AGATCGTGTG CAGTGACACC
    TGGGCCGATG AGGACCGGTG CTGTGAGCTC CCTGCCTCGA GGGATGGAGA GACCTGCCCC
    GCCTTCGTGT CTGCCTGTTC CTGTTCCTCA GAGACCCCTG GGCCCCCAGG ACCTCAAGGA
    CCCCCAGGCC TCCCTGGGAG GAATGGCACC CCAGGAGAGC AGGGCTTCCC AGGGCCCAGG
    GGAGAGCCCG GGCCACCCGG ACAGATGGGA CCAGAAGGTC CTGGAGGCCA GCAGGGCTCG
    CCGGGGACCC AGGGCCGTGC AGTCCAGGGG CCTGTGGGTC CACCAGGGGT CAAAGGAGAG
    AAGGGAGACC ATGGGCTTCC AGGCTTGCAG GGCCACCCCG GCCACCAGGG CATCCCCGGG
    AGAGTTGGCC TCCAGGGACC AAAGGGAATG AGAGGCCTGG AGGGAACTGC TGGCCTGCCT
    GGACCCCCTG GCCCCAGGGG GTTCCAGGGC ATGGCAGGGG CCAGGGGCAC TAGTGGAGAG
    CGAGGACCTC CAGGGACCGT GGGGCCCACA GGACTGCCAG GGCCCAAAGG GGAACGAGGA
    GAGAAGCACA CGTGTCAAAG TTCGACTCCT TCCACGAGAA CACCAGGCCC CCCATGCCCA
    TCTTGGAGCA GAAGCTGGAG CCGGGCACTG AGCCCCTGGG GTCCCCTGGC ACCCGCAGCA
    AGGCCCTGGT TCCTGGAGAA TGGGGGCGTG GTGGCCGCCA CCTTGAGGGC AGAGGGGAGC
    CTGGAGCTGT TGGTCAGATG GGCAGCCCTG GGCAGCAGGG GGCTAGCACC CAGGGCCTCT
    GGGAGTGACA GGACATTTTC TGCACTGCCC CGAGGAACGC TGAGCCTTCC TCCCTGGGTT
    TGTCTGGACA CCGAGAGCGA CCACATCCTG GAGAAGCCAG GAGAAAAGCT CAGGAAGAGC
    CTGCAGGTGG AAGGAGAGGG AAGCAGCGGC CTCGGCCAAG GCCCACCCCA TACTCTTGGC
    TCTGTAGCAT TTCCAAGTTC AGATAAACCC CTGAGTGCTC ACCCAGCCAA GCGAGGCGGG
    AGTCATTTCC TGGGAGAGTG TCCCCCACCC TGGCTCTGTG CACCCCACCT TGAGGCCCTG
    CAGCTTCAGC CGCAGTGGGG AAGGGGAGGG GCTGGGCTGA TGGCGAATCG GGTTCAGGAG
    GGCAGCAGTG CCCTGTGGCC GTCTCAGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70751
    Clone ID Related Accession (Same CDS sequence) XM_011528938.1
    Accession Version XM_011528938.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4080bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product collagen alpha-1(XX) chain isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGCTCCG GAGACCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
    CTGGGAAGAG AGCAAGTTCA AGCAAGCGGT CTCCTGAGGC TGGCTGTGCT GCCTGAGGAC
    CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG
    AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC
    ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC
    TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC
    CTGGACAGGA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC
    ACGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG
    GATCCGCGCA CTCCTGCCGG CCCCCAGTTC CGCTGCCTGC CCCCCGTGCC TGCTGACATG
    GTCTTCCTGG TGGACGGGTC CTGGAGCATT GGCCACAGTC ACTTCCAGCA GGTCAAGGAC
    TTCCTGGCCA GTGTCATCGC ACCCTTTGAA ATCGGGCCGG ATAAGGTCCA AGTAGGCCTG
    ACTCAGTACA GCGGGGATGC TCAGACTGAG TGGGACCTGA ACTCCCTCAG CACCAAGGAA
    CAGGTGCTGG CAGCTGTGCG CCGCCTCCGC TACAAGGGGG GGAACACGTT CACAGGCCTT
    GCCCTGACCC ACGTGCTGGG GCAGAACCTG CAGCCGGCGG CTGGCCTCCG TCCAGAGGCA
    GCCAAGGTGG TGATTCTGGT GACGGACGGC AAGTCCCAGG ACGATGTGCA CACTGCTGCC
    CGTGTCCTCA AGGACCTGGG CGTGAACGTC TTCGCTGTGG GTGTGAAGAA CGCCGATGAG
    GCTGAGCTGA GGCTCCTGGC GTCCCCGCCG AGGGACATCA CCGTCCACAG CGTGCTGGAC
    TTCCTGCAGC TCGGCGCGCT GGCTGGCCTG CTCAGCCGTC TCATCTGCCA GAGGCTCCAG
    GGTGGGAGCC CGCGGCAGGG CCCAGCAGCA GCGGCTCCAG CCCTGGACAC CCTCCCTGCC
    CCCACCAGCC TGGTCCTGAG CCAGGTGACC TCCTCCAGCA TCCGCCTGTC CTGGACTCCA
    GCCCCCCGGC ACCCCCTCAA GTATCTGATC GTTTGGCGAG CCTCTAGAGG TGGCACCCCC
    AGGGAGGTGG TGGTGGAGGG ACCCGCCGCC TCCACGGAGC TGCACAACCT GGCCTCCCGC
    ACAGAGTACC TGGTCTCCGT GTTCCCCATC TATGAGGGCG GGGTTGGCGA AGGCCTGCGG
    GGCCTGGTGA CCACAGCACC TCTGCCTCCG CCCCGGGCGC TGACCCTGGC CGCAGTGACG
    CCCAGAACCG TCCACCTCAC CTGGCAGCCC TCGGCCGGGG CCACCCACTA CCTGGTGCGA
    TGTTCTCCTG CTTCCCCCAA GGGTGAAGAG GAGGAGCGAG AGGTGCAGGT CGGGCGGCCC
    GAGGTGCTGC TGGATGGCCT GGAACCTGGC AGGGACTATG AGGTCTCGGT GCAGAGCCTG
    CGAGGCCCTG AGGGCAGCGA GGCCCGGGGC ATCCGTGCCA GGACCCCCAC CCTGGCCCCC
    CCGAGACACC TGGGCTTCTC AGACGTGAGC CACGACGCGG CACGAGTGTT CTGGGAGGGT
    GCCCCGAGGC CTGTGCGCCT GGTCAGGGTC ACCTATGTGT CCAGCGAGGG TGGACACTCG
    GGGCAGACAG AGGCTCCTGG GAACGCCACC TCGGCCACGC TGGGGCCTCT CTCTTCCTCC
    ACCACCTACA CTGTCCGTGT CACCTGCCTC TACCCTGGGG GTGGCTCCTC TACGCTGACT
    GGCCGGGTGA CCACCAAGAA AGCTCCCAGC CCAAGCCAGC TGTCCATGAC GGAGCTGCCA
    GGGGATGCAG TCCAGCTGGC GTGGGTGGCC GCAGCCCCGT CTGGCGTGCT TGTCTACCAG
    ATCACGTGGA CGCCCCTGGG AGAGGGGAAG GCTCACGAGA TCTCTGTCCC AGGGAACCTC
    GGCACGGCCG TCCTGCCTGG CCTAGGGAGG CACACAGAGT ACGACGTCAC CATCTTGGCC
    TACTACAGGG ACGGGGCCCG CAGTGACCCT GTGTCCCTCC GCTATACCCC CTCCACGGTG
    AGCAGGAGCC CACCCTCCAA CCTGGCCCTG GCCTCGGAGA CCCCCGACAG CCTGCAGGTC
    AGCTGGACGC CCCCGCTTGG CCGCGTGCTC CATTACTGGC TCACCTACGC CCCCGCCTCT
    GGCTTGGGAC CCGAGAAATC CGTCTCTGTG CCAGGAGCCA GGAGCCACGT GACACTGCCC
    GACCTGCAGG CAGCCACGAA GTACAGGGTC CTGGTCTCAG CTATCTATGC AGCAGGCAGG
    AGTGAGGCTG TGTCTGCCAC GGGCCAGACA GCAGCCTGCC CAGCCCTCCG CCCTGACGGC
    TCCCTCCCAG GGTTTGACCT GATGGTGGCC TTCAGCCTGG TGGAAAAGGC TTATGCGTCC
    ATCCGGGGCG TGGCCATGGA GCCCTCTGCC TTCGGTGGGA CCCCGACCTT CACGCTCTTC
    AAGGACGCCC AGCTGACAAG ACGGGTCAGT GACGTCTACC CAGCCCCCCT ACCTCCAGAG
    CACACCATCG TCTTCCTTGT GCGCCTACTT CCCGAGACAC CCCGTGAGGC CTTCGCGCTG
    TGGCAGATGA CAGCCGAGGA CTTCCAGCCC CTCCTTGGGG TTCTGCTGGA TGCCGGGAAG
    AAGTCCCTGA CCTACTTCCA CCGTGACCCC AGGGCTGCCT TGCAGGAGGC CACCTTCGAC
    CCGCAGGAAG TGAGGAAGAT TTTCTTCGGG AGCTTCCACA AGGTGCACGT GGCTGTGGGC
    CGCTCCAAGG TCAGGCTCTA TGTGGACTGC CGGAAGGTGG CTGAGCGGCC CCTTGGGGAG
    ATGGGCAGCC CACCCGCTGC GGGCTTCGTC ACGCTGGGGA GGCTGGCCAA GGCCAGGGGC
    CCCCGGAGCA GTTCGGCCGC GTTTCAGCTC CAGATGCTGC AGATCGTGTG CAGTGACACC
    TGGGCCGATG AGGACCGGTG CTGTGAGCTC CCTGCCTCGA GGGATGGAGA GACCTGCCCC
    GCCTTCGTGT CTGCCTGTTC CTGTTCCTCA GAGACCCCTG GGCCCCCAGG ACCTCAAGGA
    CCCCCAGGCC TCCCTGGGAG GAATGGCACC CCAGGAGAGC AGGGCTTCCC AGGGCCCAGG
    GGAGAGCCCG GGCCACCCGG ACAGATGGGA CCAGAAGGTC CTGGAGGCCA GCAGGGCTCG
    CCGGGGACCC AGGGCCGTGC AGTCCAGGGG CCTGTGGGTC CACCAGGGGT CAAAGGAGAG
    AAGGGAGACC ATGGGCTTCC AGGCTTGCAG GGCCACCCCG GCCACCAGGG CATCCCCGGG
    AGAGTTGGCC TCCAGGGACC AAAGGGAATG AGAGGCCTGG AGGGAACTGC TGGCCTGCCT
    GGACCCCCTG GCCCCAGGGG GTTCCAGGGC ATGGCAGGGG CCAGGGGCAC TAGTGGAGAG
    CGAGGACCTC CAGGGACCGT GGGGCCCACA GGACTGCCAG GGCCCAAAGG GGAACGAGGA
    GAGAAGGGCG AGCCGCAGTC CCTTGCCACC CTCTACCAGC TTGTGAGCCA GGCCTGTGAG
    TCTGCCATTC AGACACACGT GTCAAAGTTC GACTCCTTCC ACGAGAACAC CAGGCCCCCC
    ATGCCCATCT TGGAGCAGAA GCTGGAGCCG GGCACTGAGC CCCTGGGGTC CCCTGGCACC
    CGCAGCAAGG CCCTGGTTCC TGGAGAATGG GGGCGTGGTG GCCGCCACCT TGAGGGCAGA
    GCAGCGGTGG GTCATGACTT GGACGCTGAG ATGGAGGAGA GGCTGATTCG GGGAGCTGGA
    GCTGGAGAGG GGCGTCCACG GGGAGAACAG ACGGGGGATG ACCCAGCCAG ACGAGGACTG
    AGTTCTGCAC TGGCCGGGGC AGCAGCGGGG GCATCTCTGC TCTGTTCGGG CATCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011527240.1
    CDS118..4197
    Translation

    Target ORF information:

    RefSeq Version XM_011528938.1
    Organism Homo sapiens(human)
    Definition Homo sapiens collagen type XX alpha 1 chain (COL20A1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011528938.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAGCTCCG GAGACCCTGC ACACCTCGGC CTCTGCCTCT GGCTGTGGCT GGGCGCCACC 
    CTGGGAAGAG AGCAAGTTCA AGCAAGCGGT CTCCTGAGGC TGGCTGTGCT GCCTGAGGAC
    CGGCTGCAGA TGAAGTGGAG AGAGTCGGAG GGGAGCGGCC TCGGCTACCT GGTGCAGGTG
    AAGCCCATGG CAGGGGACTC GGAACAGGAG GTGATACTGA CCACCAAGAC CCCTAAGGCC
    ACAGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCTTGC AGATCTTCGA GCTCACTGGC
    TCTGGGCGCT TCCTGCTAGC TCGGAGGGAG TTTGTGATTG AGGATCTGAA GAGTAGCTCC
    CTGGACAGGA GCAGCCAGAG GCCCCTCGGC TCTGGAGCCC CGGAGCCCAC CCCCTCCCAC
    ACGGGGAGCC CAGACCCTGA GCAGGCTTCT GAGCCCCAAG TTGCCTTCAC ACCAAGCCAG
    GATCCGCGCA CTCCTGCCGG CCCCCAGTTC CGCTGCCTGC CCCCCGTGCC TGCTGACATG
    GTCTTCCTGG TGGACGGGTC CTGGAGCATT GGCCACAGTC ACTTCCAGCA GGTCAAGGAC
    TTCCTGGCCA GTGTCATCGC ACCCTTTGAA ATCGGGCCGG ATAAGGTCCA AGTAGGCCTG
    ACTCAGTACA GCGGGGATGC TCAGACTGAG TGGGACCTGA ACTCCCTCAG CACCAAGGAA
    CAGGTGCTGG CAGCTGTGCG CCGCCTCCGC TACAAGGGGG GGAACACGTT CACAGGCCTT
    GCCCTGACCC ACGTGCTGGG GCAGAACCTG CAGCCGGCGG CTGGCCTCCG TCCAGAGGCA
    GCCAAGGTGG TGATTCTGGT GACGGACGGC AAGTCCCAGG ACGATGTGCA CACTGCTGCC
    CGTGTCCTCA AGGACCTGGG CGTGAACGTC TTCGCTGTGG GTGTGAAGAA CGCCGATGAG
    GCTGAGCTGA GGCTCCTGGC GTCCCCGCCG AGGGACATCA CCGTCCACAG CGTGCTGGAC
    TTCCTGCAGC TCGGCGCGCT GGCTGGCCTG CTCAGCCGTC TCATCTGCCA GAGGCTCCAG
    GGTGGGAGCC CGCGGCAGGG CCCAGCAGCA GCGGCTCCAG CCCTGGACAC CCTCCCTGCC
    CCCACCAGCC TGGTCCTGAG CCAGGTGACC TCCTCCAGCA TCCGCCTGTC CTGGACTCCA
    GCCCCCCGGC ACCCCCTCAA GTATCTGATC GTTTGGCGAG CCTCTAGAGG TGGCACCCCC
    AGGGAGGTGG TGGTGGAGGG ACCCGCCGCC TCCACGGAGC TGCACAACCT GGCCTCCCGC
    ACAGAGTACC TGGTCTCCGT GTTCCCCATC TATGAGGGCG GGGTTGGCGA AGGCCTGCGG
    GGCCTGGTGA CCACAGCACC TCTGCCTCCG CCCCGGGCGC TGACCCTGGC CGCAGTGACG
    CCCAGAACCG TCCACCTCAC CTGGCAGCCC TCGGCCGGGG CCACCCACTA CCTGGTGCGA
    TGTTCTCCTG CTTCCCCCAA GGGTGAAGAG GAGGAGCGAG AGGTGCAGGT CGGGCGGCCC
    GAGGTGCTGC TGGATGGCCT GGAACCTGGC AGGGACTATG AGGTCTCGGT GCAGAGCCTG
    CGAGGCCCTG AGGGCAGCGA GGCCCGGGGC ATCCGTGCCA GGACCCCCAC CCTGGCCCCC
    CCGAGACACC TGGGCTTCTC AGACGTGAGC CACGACGCGG CACGAGTGTT CTGGGAGGGT
    GCCCCGAGGC CTGTGCGCCT GGTCAGGGTC ACCTATGTGT CCAGCGAGGG TGGACACTCG
    GGGCAGACAG AGGCTCCTGG GAACGCCACC TCGGCCACGC TGGGGCCTCT CTCTTCCTCC
    ACCACCTACA CTGTCCGTGT CACCTGCCTC TACCCTGGGG GTGGCTCCTC TACGCTGACT
    GGCCGGGTGA CCACCAAGAA AGCTCCCAGC CCAAGCCAGC TGTCCATGAC GGAGCTGCCA
    GGGGATGCAG TCCAGCTGGC GTGGGTGGCC GCAGCCCCGT CTGGCGTGCT TGTCTACCAG
    ATCACGTGGA CGCCCCTGGG AGAGGGGAAG GCTCACGAGA TCTCTGTCCC AGGGAACCTC
    GGCACGGCCG TCCTGCCTGG CCTAGGGAGG CACACAGAGT ACGACGTCAC CATCTTGGCC
    TACTACAGGG ACGGGGCCCG CAGTGACCCT GTGTCCCTCC GCTATACCCC CTCCACGGTG
    AGCAGGAGCC CACCCTCCAA CCTGGCCCTG GCCTCGGAGA CCCCCGACAG CCTGCAGGTC
    AGCTGGACGC CCCCGCTTGG CCGCGTGCTC CATTACTGGC TCACCTACGC CCCCGCCTCT
    GGCTTGGGAC CCGAGAAATC CGTCTCTGTG CCAGGAGCCA GGAGCCACGT GACACTGCCC
    GACCTGCAGG CAGCCACGAA GTACAGGGTC CTGGTCTCAG CTATCTATGC AGCAGGCAGG
    AGTGAGGCTG TGTCTGCCAC GGGCCAGACA GCAGCCTGCC CAGCCCTCCG CCCTGACGGC
    TCCCTCCCAG GGTTTGACCT GATGGTGGCC TTCAGCCTGG TGGAAAAGGC TTATGCGTCC
    ATCCGGGGCG TGGCCATGGA GCCCTCTGCC TTCGGTGGGA CCCCGACCTT CACGCTCTTC
    AAGGACGCCC AGCTGACAAG ACGGGTCAGT GACGTCTACC CAGCCCCCCT ACCTCCAGAG
    CACACCATCG TCTTCCTTGT GCGCCTACTT CCCGAGACAC CCCGTGAGGC CTTCGCGCTG
    TGGCAGATGA CAGCCGAGGA CTTCCAGCCC CTCCTTGGGG TTCTGCTGGA TGCCGGGAAG
    AAGTCCCTGA CCTACTTCCA CCGTGACCCC AGGGCTGCCT TGCAGGAGGC CACCTTCGAC
    CCGCAGGAAG TGAGGAAGAT TTTCTTCGGG AGCTTCCACA AGGTGCACGT GGCTGTGGGC
    CGCTCCAAGG TCAGGCTCTA TGTGGACTGC CGGAAGGTGG CTGAGCGGCC CCTTGGGGAG
    ATGGGCAGCC CACCCGCTGC GGGCTTCGTC ACGCTGGGGA GGCTGGCCAA GGCCAGGGGC
    CCCCGGAGCA GTTCGGCCGC GTTTCAGCTC CAGATGCTGC AGATCGTGTG CAGTGACACC
    TGGGCCGATG AGGACCGGTG CTGTGAGCTC CCTGCCTCGA GGGATGGAGA GACCTGCCCC
    GCCTTCGTGT CTGCCTGTTC CTGTTCCTCA GAGACCCCTG GGCCCCCAGG ACCTCAAGGA
    CCCCCAGGCC TCCCTGGGAG GAATGGCACC CCAGGAGAGC AGGGCTTCCC AGGGCCCAGG
    GGAGAGCCCG GGCCACCCGG ACAGATGGGA CCAGAAGGTC CTGGAGGCCA GCAGGGCTCG
    CCGGGGACCC AGGGCCGTGC AGTCCAGGGG CCTGTGGGTC CACCAGGGGT CAAAGGAGAG
    AAGGGAGACC ATGGGCTTCC AGGCTTGCAG GGCCACCCCG GCCACCAGGG CATCCCCGGG
    AGAGTTGGCC TCCAGGGACC AAAGGGAATG AGAGGCCTGG AGGGAACTGC TGGCCTGCCT
    GGACCCCCTG GCCCCAGGGG GTTCCAGGGC ATGGCAGGGG CCAGGGGCAC TAGTGGAGAG
    CGAGGACCTC CAGGGACCGT GGGGCCCACA GGACTGCCAG GGCCCAAAGG GGAACGAGGA
    GAGAAGGGCG AGCCGCAGTC CCTTGCCACC CTCTACCAGC TTGTGAGCCA GGCCTGTGAG
    TCTGCCATTC AGACACACGT GTCAAAGTTC GACTCCTTCC ACGAGAACAC CAGGCCCCCC
    ATGCCCATCT TGGAGCAGAA GCTGGAGCCG GGCACTGAGC CCCTGGGGTC CCCTGGCACC
    CGCAGCAAGG CCCTGGTTCC TGGAGAATGG GGGCGTGGTG GCCGCCACCT TGAGGGCAGA
    GCAGCGGTGG GTCATGACTT GGACGCTGAG ATGGAGGAGA GGCTGATTCG GGGAGCTGGA
    GCTGGAGAGG GGCGTCCACG GGGAGAACAG ACGGGGGATG ACCCAGCCAG ACGAGGACTG
    AGTTCTGCAC TGGCCGGGGC AGCAGCGGGG GCATCTCTGC TCTGTTCGGG CATCGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.