VPS18 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS18
Entrez Gene ID	57617
Full Name	VPS18, CORVET/HOPS core subunit
Synonyms	PEP3
General protein information	Preferred Names VPS18, CORVET/HOPS core subunit Names vacuolar protein sorting-associated protein 18 homolog hVPS18 vacuolar protein sorting 18 homolog vacuolar protein sorting protein 18
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 15 Map Location: 15q15.1
Summary	Vesicle mediated protein sorting plays an important role in segregation of intracellular molecules into distinct organelles. Genetic studies in yeast have identified more than 40 vacuolar protein sorting (VPS) genes involved in vesicle transport to vacuoles. This gene encodes the human homolog of yeast class C Vps18 protein. The mammalian class C Vps proteins are predominantly associated with late endosomes/lysosomes, and like their yeast counterparts, may mediate vesicle trafficking steps in the endosome/lysosome pathway. [provided by RefSeq, Jul 2008].
Disorder MIM:	608551

mRNA and Protein(s)

mRNA	Protein	Name
NM_020857.2	NP_065908.1	vacuolar protein sorting-associated protein 18 homolog
NM_020857.3	NP_065908.1	vacuolar protein sorting-associated protein 18 homolog
XM_017022447.1	XP_016877936.1	vacuolar protein sorting-associated protein 18 homolog isoform X3
XM_011521843.2	XP_011520145.1	vacuolar protein sorting-associated protein 18 homolog isoform X1
XM_011521844.2	XP_011520146.1	vacuolar protein sorting-associated protein 18 homolog isoform X2

Pathways from BioSystems

Homologies

Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000983	XP_560205.4
Magnaporthe oryzae (rice blast fungus)	MGG_06325	XP_003717259.1
Schizosaccharomyces pombe (fission yeast)	pep3	NP_588498.1
Arabidopsis thaliana (thale cress)	AT1G12470	NP_172709.2
Homo sapiens (human)	VPS18	NP_065908.1
Mus musculus (house mouse)	Vps18	NP_758473.3
Rattus norvegicus (Norway rat)	Vps18	NP_001099969.1
Gallus gallus (chicken)	VPS18	NP_001186584.1
Drosophila melanogaster (fruit fly)	dor	NP_477286.2
Xenopus tropicalis (tropical clawed frog)	vps18	NP_001121454.1
	VPS18	XP_001099146.1
Canis lupus familiaris (dog)	VPS18	XP_544627.3
Oryza sativa (rice)	Os08g0178100	NP_001061126.1
Pan troglodytes (chimpanzee)	VPS18	XP_523187.2
Bos taurus (cattle)	VPS18	NP_001093850.1
Danio rerio (zebrafish)	vps18	NP_775352.2
Neurospora crassa	NCU06183	XP_962995.2

Gene Ontology
Bibliography

Related articles in PubMed

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.
Hunter MR, Scourfield EJ, Emmott E, Graham SC
The Biochemical journal474(21)3615-3626(2017 10)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.
van der Kant R, Jonker CT, Wijdeven RH, Bakker J, Janssen L, Klumperman J, Neefjes J
The Journal of biological chemistry290(51)30280-90(2015 Dec)

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Hein MY, Hubner NC, Poser I, Cox J, Nagaraj N, Toyoda Y, Gak IA, Weisswange I, Mansfeld J, Buchholz F, Hyman AA, Mann M
Cell163(3)712-23(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction
Title: VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.

Depletion of hVps18 or hMon2 reduced the efficient production of infectious HIV-1 virions in human cells.
Title: The cellular factors Vps18 and Mon2 are required for efficient production of infectious HIV-1 particles.

ubiquitin binding and ubiquitylation of GGA3-GAT domain are mutually inseparable through a ubiquitin ligase activity of hVPS18
Title: Monoubiquitylation of GGA3 by hVPS18 regulates its ubiquitin-binding ability.

hVPS18 may play an important role in regulation of SNK activity through its ubiquitin ligase
Title: Ubiquitylation and degradation of serum-inducible kinase by hVPS18, a RING-H2 type ubiquitin ligase.

The following VPS18 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS18 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu30825	NM_020857.3 Latest version!	Homo sapiens VPS18 core subunit of CORVET and HOPS complexes (VPS18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu70724	XM_011521843.2 Latest version!	Homo sapiens VPS18 core subunit of CORVET and HOPS complexes (VPS18), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu70725	XM_011521844.2 Latest version!	Homo sapiens VPS18 core subunit of CORVET and HOPS complexes (VPS18), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu85578	XM_017022447.1 Latest version!	Homo sapiens VPS18 core subunit of CORVET and HOPS complexes (VPS18), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$307.30 $439.00
View Old Accession Versions >>
OHu30825	NM_020857.2	Homo sapiens VPS18, CORVET/HOPS core subunit (VPS18), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS18 ( NM_020857.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_065908.1 Homo sapiens VPS18, CORVET/HOPS core subunit (VPS18), mRNA.

Price & Availability↑

CloneID	OHu30825
Clone ID Related Accession (Same CDS sequence)	NM_020857.2 , NM_020857.3
Accession Version	NM_020857.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-31
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 18 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL713725.1 and AF308802.1. On or before Mar 23, 2004 this sequence version replaced XM_378171.1, NM_080432.1, NM_020857.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL713725.1, SRR1803612.186350.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGTCCA TCCTGGATGA GTACGAGAAC TCGCTGTCCC GCTCGGCCGT CTTGCAGCCC 
GGCTGCCCTA GCGTGGGCAT CCCCCACTCG GGGTATGTGA ATGCCCAGCT GGAGAAGGAA 
GTGCCCATCT TCACAAAGCA GCGCATTGAC TTCACCCCTT CCGAGCGCAT TACCAGTCTT 
GTCGTCTCCA GCAATCAGCT GTGCATGAGC CTGGGCAAGG ATACACTGCT CCGCATTGAC 
TTGGGCAAGG CAAATGAGCC CAACCACGTG GAGCTGGGAC GTAAGGATGA CGCAAAAGTT 
CACAAGATGT TCCTTGACCA TACTGGCTCT CACCTGCTGA TTGCCCTGAG CAGCACGGAG 
GTCCTCTACG TGAACCGAAA TGGACAGAAG GTACGGCCAC TAGCACGCTG GAAGGGGCAG 
CTGGTGGAGA GTGTGGGTTG GAACAAGGCA CTGGGCACGG AGAGCAGCAC AGGCCCCATC 
CTGGTCGGGA CTGCCCAAGG CCACATCTTT GAAGCAGAGC TCTCAGCCAG CGAAGGTGGG 
CTTTTCGGCC CTGCTCCGGA TCTCTACTTC CGCCCATTGT ACGTGCTAAA TGAAGAAGGG 
GGTCCAGCAC CTGTGTGCTC CCTTGAGGCC GAGCGGGGCC CTGATGGGCG TAGCTTTGTT 
ATTGCCACCA CTCGGCAGCG CCTCTTCCAG TTCATAGGCC GAGCAGCAGA GGGGGCTGAG 
GCCCAGGGTT TCTCAGGGCT CTTTGCAGCT TACACGGACC ACCCACCCCC ATTCCGTGAG 
TTTCCCAGCA ACCTGGGCTA CAGTGAGTTG GCCTTCTACA CCCCCAAGCT GCGCTCCGCA 
CCCCGGGCCT TCGCCTGGAT GATGGGGGAT GGTGTGTTGT ATGGGGCATT GGACTGTGGG 
CGCCCTGACT CTCTGCTGAG CGAGGAGCGA GTCTGGGAGT ACCCAGAGGG GGTAGGGCCT 
GGGGCCAGCC CACCCCTAGC CATCGTCTTG ACCCAGTTCC ACTTCCTGCT GCTACTGGCA 
GACCGGGTGG AGGCAGTGTG CACACTGACC GGGCAGGTGG TGCTGCGGGA TCACTTCCTG 
GAGAAATTTG GGCCGCTGAA GCACATGGTG AAGGACTCCT CCACAGGCCA GCTGTGGGCC 
TACACTGAGC GGGCTGTCTT CCGCTACCAC GTGCAACGGG AGGCCCGAGA TGTCTGGCGC 
ACCTATCTGG ACATGAACCG CTTCGATCTG GCCAAAGAGT ATTGTCGAGA GCGGCCCGAC 
TGCCTGGACA CGGTCCTGGC CCGGGAGGCC GATTTCTGCT TTCGCCAGCG TCGCTACCTG 
GAGAGCGCAC GCTGCTATGC CCTGACCCAG AGCTACTTTG AGGAGATTGC CCTCAAGTTC 
CTGGAGGCCC GACAGGAGGA GGCTCTGGCT GAGTTCCTGC AGCGAAAACT GGCCAGTTTG 
AAGCCAGCCG AACGTACCCA GGCCACACTG CTGACCACCT GGCTGACAGA GCTCTACCTG 
AGCCGGCTTG GGGCTCTGCA GGGCGACCCA GAGGCCCTGA CTCTCTACCG AGAAACCAAG 
GAATGCTTTC GAACCTTCCT CAGCAGCCCC CGCCACAAAG AGTGGCTCTT TGCCAGCCGG 
GCCTCTATCC ATGAGCTGCT CGCCAGTCAT GGGGACACAG AACACATGGT GTACTTTGCA 
GTGATCATGC AGGACTATGA GCGGGTGGTG GCTTACCACT GTCAGCACGA GGCCTACGAG 
GAGGCCCTGG CCGTGCTCGC CCGCCACCGT GACCCCCAGC TCTTCTACAA GTTCTCACCC 
ATCCTCATCC GTCACATCCC CCGCCAGCTT GTAGATGCCT GGATTGAGAT GGGCAGCCGG 
CTGGATGCTC GTCAGCTCAT TCCTGCCCTG GTGAACTACA GCCAGGGTGG TGAGGTCCAG 
CAGGTGAGCC AGGCCATCCG CTACATGGAG TTCTGCGTGA ACGTGCTGGG GGAGACTGAG 
CAGGCCATCC ACAACTACCT GCTGTCACTG TATGCCCGTG GCCGGCCGGA CTCACTACTG 
GCCTATCTGG AGCAGGCTGG GGCCAGCCCC CACCGGGTGC ATTACGACCT CAAGTATGCG 
CTGCGGCTCT GCGCCGAGCA TGGCCACCAC CGCGCTTGTG TCCATGTCTA CAAGGTCCTA 
GAGCTGTATG AGGAGGCCGT GGACCTGGCC CTGCAGGTGG ATGTGGACCT GGCCAAGCAG 
TGTGCAGACC TGCCTGAGGA GGATGAGGAA TTGCGCAAGA AGCTGTGGCT GAAGATCGCA 
CGGCACGTGG TGCAGGAAGA GGAAGATGTA CAGACAGCCA TGGCTTGCCT GGCTAGCTGC 
CCCTTGCTCA AGATTGAGGA TGTGCTGCCC TTCTTTCCTG ATTTCGTCAC CATCGACCAC 
TTCAAGGAGG CGATCTGCAG CTCACTTAAG GCCTACAACC ACCACATCCA GGAGCTGCAG 
CGGGAGATGG AAGAGGCTAC AGCCAGTGCC CAGCGCATCC GGCGAGACCT GCAGGAGCTG 
CGGGGCCGCT ACGGCACTGT GGAGCCCCAG GACAAATGTG CCACCTGCGA CTTCCCCCTG 
CTCAACCGCC CTTTTTACCT CTTCCTCTGT GGCCATATGT TCCATGCTGA CTGCCTGCTG 
CAGGCTGTGC GACCTGGCCT GCCAGCCTAC AAGCAGGCCC GGCTGGAGGA GCTGCAGAGG 
AAGCTGGGGG CTGCTCCACC CCCAGCCAAG GGCTCTGCCC GGGCCAAGGA GGCCGAGGGT 
GGGGCTGCCA CGGCAGGGCC CAGCCGGGAA CAGCTCAAGG CTGACCTGGA TGAGTTGGTG 
GCCGCTGAGT GTGTGTACTG TGGGGAGCTG ATGATCCGCT CTATCGACCG GCCGTTCATC 
GACCCCCAGC GCTACGAGGA GGAGCAGCTC AGTTGGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065908.1
CDS	340..3261
Translation	MASILDEYENSLSRSAVLQPGCPSVGIPHSGYVNAQLEKEVPIFTKQRIDFTPSERITSLVVSSNQLCMSLGKDTLLRIDLGKANEPNHVELGRKDDAKVHKMFLDHTGSHLLIALSSTEVLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGHIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRSFVIATTRQRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSWL

Target ORF information:

RefSeq Version	NM_020857.2
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS18, CORVET/HOPS core subunit (VPS18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020857.2

ORF Insert Sequence:

ATGGCGTCCA TCCTGGATGA GTACGAGAAC TCGCTGTCCC GCTCGGCCGT CTTGCAGCCC 
GGCTGCCCTA GCGTGGGCAT CCCCCACTCG GGGTATGTGA ATGCCCAGCT GGAGAAGGAA 
GTGCCCATCT TCACAAAGCA GCGCATTGAC TTCACCCCTT CCGAGCGCAT TACCAGTCTT 
GTCGTCTCCA GCAATCAGCT GTGCATGAGC CTGGGCAAGG ATACACTGCT CCGCATTGAC 
TTGGGCAAGG CAAATGAGCC CAACCACGTG GAGCTGGGAC GTAAGGATGA CGCAAAAGTT 
CACAAGATGT TCCTTGACCA TACTGGCTCT CACCTGCTGA TTGCCCTGAG CAGCACGGAG 
GTCCTCTACG TGAACCGAAA TGGACAGAAG GTACGGCCAC TAGCACGCTG GAAGGGGCAG 
CTGGTGGAGA GTGTGGGTTG GAACAAGGCA CTGGGCACGG AGAGCAGCAC AGGCCCCATC 
CTGGTCGGGA CTGCCCAAGG CCACATCTTT GAAGCAGAGC TCTCAGCCAG CGAAGGTGGG 
CTTTTCGGCC CTGCTCCGGA TCTCTACTTC CGCCCATTGT ACGTGCTAAA TGAAGAAGGG 
GGTCCAGCAC CTGTGTGCTC CCTTGAGGCC GAGCGGGGCC CTGATGGGCG TAGCTTTGTT 
ATTGCCACCA CTCGGCAGCG CCTCTTCCAG TTCATAGGCC GAGCAGCAGA GGGGGCTGAG 
GCCCAGGGTT TCTCAGGGCT CTTTGCAGCT TACACGGACC ACCCACCCCC ATTCCGTGAG 
TTTCCCAGCA ACCTGGGCTA CAGTGAGTTG GCCTTCTACA CCCCCAAGCT GCGCTCCGCA 
CCCCGGGCCT TCGCCTGGAT GATGGGGGAT GGTGTGTTGT ATGGGGCATT GGACTGTGGG 
CGCCCTGACT CTCTGCTGAG CGAGGAGCGA GTCTGGGAGT ACCCAGAGGG GGTAGGGCCT 
GGGGCCAGCC CACCCCTAGC CATCGTCTTG ACCCAGTTCC ACTTCCTGCT GCTACTGGCA 
GACCGGGTGG AGGCAGTGTG CACACTGACC GGGCAGGTGG TGCTGCGGGA TCACTTCCTG 
GAGAAATTTG GGCCGCTGAA GCACATGGTG AAGGACTCCT CCACAGGCCA GCTGTGGGCC 
TACACTGAGC GGGCTGTCTT CCGCTACCAC GTGCAACGGG AGGCCCGAGA TGTCTGGCGC 
ACCTATCTGG ACATGAACCG CTTCGATCTG GCCAAAGAGT ATTGTCGAGA GCGGCCCGAC 
TGCCTGGACA CGGTCCTGGC CCGGGAGGCC GATTTCTGCT TTCGCCAGCG TCGCTACCTG 
GAGAGCGCAC GCTGCTATGC CCTGACCCAG AGCTACTTTG AGGAGATTGC CCTCAAGTTC 
CTGGAGGCCC GACAGGAGGA GGCTCTGGCT GAGTTCCTGC AGCGAAAACT GGCCAGTTTG 
AAGCCAGCCG AACGTACCCA GGCCACACTG CTGACCACCT GGCTGACAGA GCTCTACCTG 
AGCCGGCTTG GGGCTCTGCA GGGCGACCCA GAGGCCCTGA CTCTCTACCG AGAAACCAAG 
GAATGCTTTC GAACCTTCCT CAGCAGCCCC CGCCACAAAG AGTGGCTCTT TGCCAGCCGG 
GCCTCTATCC ATGAGCTGCT CGCCAGTCAT GGGGACACAG AACACATGGT GTACTTTGCA 
GTGATCATGC AGGACTATGA GCGGGTGGTG GCTTACCACT GTCAGCACGA GGCCTACGAG 
GAGGCCCTGG CCGTGCTCGC CCGCCACCGT GACCCCCAGC TCTTCTACAA GTTCTCACCC 
ATCCTCATCC GTCACATCCC CCGCCAGCTT GTAGATGCCT GGATTGAGAT GGGCAGCCGG 
CTGGATGCTC GTCAGCTCAT TCCTGCCCTG GTGAACTACA GCCAGGGTGG TGAGGTCCAG 
CAGGTGAGCC AGGCCATCCG CTACATGGAG TTCTGCGTGA ACGTGCTGGG GGAGACTGAG 
CAGGCCATCC ACAACTACCT GCTGTCACTG TATGCCCGTG GCCGGCCGGA CTCACTACTG 
GCCTATCTGG AGCAGGCTGG GGCCAGCCCC CACCGGGTGC ATTACGACCT CAAGTATGCG 
CTGCGGCTCT GCGCCGAGCA TGGCCACCAC CGCGCTTGTG TCCATGTCTA CAAGGTCCTA 
GAGCTGTATG AGGAGGCCGT GGACCTGGCC CTGCAGGTGG ATGTGGACCT GGCCAAGCAG 
TGTGCAGACC TGCCTGAGGA GGATGAGGAA TTGCGCAAGA AGCTGTGGCT GAAGATCGCA 
CGGCACGTGG TGCAGGAAGA GGAAGATGTA CAGACAGCCA TGGCTTGCCT GGCTAGCTGC 
CCCTTGCTCA AGATTGAGGA TGTGCTGCCC TTCTTTCCTG ATTTCGTCAC CATCGACCAC 
TTCAAGGAGG CGATCTGCAG CTCACTTAAG GCCTACAACC ACCACATCCA GGAGCTGCAG 
CGGGAGATGG AAGAGGCTAC AGCCAGTGCC CAGCGCATCC GGCGAGACCT GCAGGAGCTG 
CGGGGCCGCT ACGGCACTGT GGAGCCCCAG GACAAATGTG CCACCTGCGA CTTCCCCCTG 
CTCAACCGCC CTTTTTACCT CTTCCTCTGT GGCCATATGT TCCATGCTGA CTGCCTGCTG 
CAGGCTGTGC GACCTGGCCT GCCAGCCTAC AAGCAGGCCC GGCTGGAGGA GCTGCAGAGG 
AAGCTGGGGG CTGCTCCACC CCCAGCCAAG GGCTCTGCCC GGGCCAAGGA GGCCGAGGGT 
GGGGCTGCCA CGGCAGGGCC CAGCCGGGAA CAGCTCAAGG CTGACCTGGA TGAGTTGGTG 
GCCGCTGAGT GTGTGTACTG TGGGGAGCTG ATGATCCGCT CTATCGACCG GCCGTTCATC 
GACCCCCAGC GCTACGAGGA GGAGCAGCTC AGTTGGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS18 ( NM_020857.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_065908.1 Homo sapiens VPS18 core subunit of CORVET and HOPS complexes (VPS18), mRNA.

Price & Availability↑

CloneID	OHu30825
Clone ID Related Accession (Same CDS sequence)	NM_020857.2 , NM_020857.3
Accession Version	NM_020857.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2922bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 18 homolog
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL713725.1 and AF308802.1. On Nov 24, 2018 this sequence version replaced NM_020857.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL713725.1, AB040908.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000220509.10/ ENSP00000220509.5 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGTCCA TCCTGGATGA GTACGAGAAC TCGCTGTCCC GCTCGGCCGT CTTGCAGCCC 
GGCTGCCCTA GCGTGGGCAT CCCCCACTCG GGGTATGTGA ATGCCCAGCT GGAGAAGGAA 
GTGCCCATCT TCACAAAGCA GCGCATTGAC TTCACCCCTT CCGAGCGCAT TACCAGTCTT 
GTCGTCTCCA GCAATCAGCT GTGCATGAGC CTGGGCAAGG ATACACTGCT CCGCATTGAC 
TTGGGCAAGG CAAATGAGCC CAACCACGTG GAGCTGGGAC GTAAGGATGA CGCAAAAGTT 
CACAAGATGT TCCTTGACCA TACTGGCTCT CACCTGCTGA TTGCCCTGAG CAGCACGGAG 
GTCCTCTACG TGAACCGAAA TGGACAGAAG GTACGGCCAC TAGCACGCTG GAAGGGGCAG 
CTGGTGGAGA GTGTGGGTTG GAACAAGGCA CTGGGCACGG AGAGCAGCAC AGGCCCCATC 
CTGGTCGGGA CTGCCCAAGG CCACATCTTT GAAGCAGAGC TCTCAGCCAG CGAAGGTGGG 
CTTTTCGGCC CTGCTCCGGA TCTCTACTTC CGCCCATTGT ACGTGCTAAA TGAAGAAGGG 
GGTCCAGCAC CTGTGTGCTC CCTTGAGGCC GAGCGGGGCC CTGATGGGCG TAGCTTTGTT 
ATTGCCACCA CTCGGCAGCG CCTCTTCCAG TTCATAGGCC GAGCAGCAGA GGGGGCTGAG 
GCCCAGGGTT TCTCAGGGCT CTTTGCAGCT TACACGGACC ACCCACCCCC ATTCCGTGAG 
TTTCCCAGCA ACCTGGGCTA CAGTGAGTTG GCCTTCTACA CCCCCAAGCT GCGCTCCGCA 
CCCCGGGCCT TCGCCTGGAT GATGGGGGAT GGTGTGTTGT ATGGGGCATT GGACTGTGGG 
CGCCCTGACT CTCTGCTGAG CGAGGAGCGA GTCTGGGAGT ACCCAGAGGG GGTAGGGCCT 
GGGGCCAGCC CACCCCTAGC CATCGTCTTG ACCCAGTTCC ACTTCCTGCT GCTACTGGCA 
GACCGGGTGG AGGCAGTGTG CACACTGACC GGGCAGGTGG TGCTGCGGGA TCACTTCCTG 
GAGAAATTTG GGCCGCTGAA GCACATGGTG AAGGACTCCT CCACAGGCCA GCTGTGGGCC 
TACACTGAGC GGGCTGTCTT CCGCTACCAC GTGCAACGGG AGGCCCGAGA TGTCTGGCGC 
ACCTATCTGG ACATGAACCG CTTCGATCTG GCCAAAGAGT ATTGTCGAGA GCGGCCCGAC 
TGCCTGGACA CGGTCCTGGC CCGGGAGGCC GATTTCTGCT TTCGCCAGCG TCGCTACCTG 
GAGAGCGCAC GCTGCTATGC CCTGACCCAG AGCTACTTTG AGGAGATTGC CCTCAAGTTC 
CTGGAGGCCC GACAGGAGGA GGCTCTGGCT GAGTTCCTGC AGCGAAAACT GGCCAGTTTG 
AAGCCAGCCG AACGTACCCA GGCCACACTG CTGACCACCT GGCTGACAGA GCTCTACCTG 
AGCCGGCTTG GGGCTCTGCA GGGCGACCCA GAGGCCCTGA CTCTCTACCG AGAAACCAAG 
GAATGCTTTC GAACCTTCCT CAGCAGCCCC CGCCACAAAG AGTGGCTCTT TGCCAGCCGG 
GCCTCTATCC ATGAGCTGCT CGCCAGTCAT GGGGACACAG AACACATGGT GTACTTTGCA 
GTGATCATGC AGGACTATGA GCGGGTGGTG GCTTACCACT GTCAGCACGA GGCCTACGAG 
GAGGCCCTGG CCGTGCTCGC CCGCCACCGT GACCCCCAGC TCTTCTACAA GTTCTCACCC 
ATCCTCATCC GTCACATCCC CCGCCAGCTT GTAGATGCCT GGATTGAGAT GGGCAGCCGG 
CTGGATGCTC GTCAGCTCAT TCCTGCCCTG GTGAACTACA GCCAGGGTGG TGAGGTCCAG 
CAGGTGAGCC AGGCCATCCG CTACATGGAG TTCTGCGTGA ACGTGCTGGG GGAGACTGAG 
CAGGCCATCC ACAACTACCT GCTGTCACTG TATGCCCGTG GCCGGCCGGA CTCACTACTG 
GCCTATCTGG AGCAGGCTGG GGCCAGCCCC CACCGGGTGC ATTACGACCT CAAGTATGCG 
CTGCGGCTCT GCGCCGAGCA TGGCCACCAC CGCGCTTGTG TCCATGTCTA CAAGGTCCTA 
GAGCTGTATG AGGAGGCCGT GGACCTGGCC CTGCAGGTGG ATGTGGACCT GGCCAAGCAG 
TGTGCAGACC TGCCTGAGGA GGATGAGGAA TTGCGCAAGA AGCTGTGGCT GAAGATCGCA 
CGGCACGTGG TGCAGGAAGA GGAAGATGTA CAGACAGCCA TGGCTTGCCT GGCTAGCTGC 
CCCTTGCTCA AGATTGAGGA TGTGCTGCCC TTCTTTCCTG ATTTCGTCAC CATCGACCAC 
TTCAAGGAGG CGATCTGCAG CTCACTTAAG GCCTACAACC ACCACATCCA GGAGCTGCAG 
CGGGAGATGG AAGAGGCTAC AGCCAGTGCC CAGCGCATCC GGCGAGACCT GCAGGAGCTG 
CGGGGCCGCT ACGGCACTGT GGAGCCCCAG GACAAATGTG CCACCTGCGA CTTCCCCCTG 
CTCAACCGCC CTTTTTACCT CTTCCTCTGT GGCCATATGT TCCATGCTGA CTGCCTGCTG 
CAGGCTGTGC GACCTGGCCT GCCAGCCTAC AAGCAGGCCC GGCTGGAGGA GCTGCAGAGG 
AAGCTGGGGG CTGCTCCACC CCCAGCCAAG GGCTCTGCCC GGGCCAAGGA GGCCGAGGGT 
GGGGCTGCCA CGGCAGGGCC CAGCCGGGAA CAGCTCAAGG CTGACCTGGA TGAGTTGGTG 
GCCGCTGAGT GTGTGTACTG TGGGGAGCTG ATGATCCGCT CTATCGACCG GCCGTTCATC 
GACCCCCAGC GCTACGAGGA GGAGCAGCTC AGTTGGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_065908.1
CDS	320..3241
Translation	MASILDEYENSLSRSAVLQPGCPSVGIPHSGYVNAQLEKEVPIFTKQRIDFTPSERITSLVVSSNQLCMSLGKDTLLRIDLGKANEPNHVELGRKDDAKVHKMFLDHTGSHLLIALSSTEVLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGHIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRSFVIATTRQRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSWL

Target ORF information:

RefSeq Version	NM_020857.3
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS18 core subunit of CORVET and HOPS complexes (VPS18), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_020857.3

ORF Insert Sequence:

ATGGCGTCCA TCCTGGATGA GTACGAGAAC TCGCTGTCCC GCTCGGCCGT CTTGCAGCCC 
GGCTGCCCTA GCGTGGGCAT CCCCCACTCG GGGTATGTGA ATGCCCAGCT GGAGAAGGAA 
GTGCCCATCT TCACAAAGCA GCGCATTGAC TTCACCCCTT CCGAGCGCAT TACCAGTCTT 
GTCGTCTCCA GCAATCAGCT GTGCATGAGC CTGGGCAAGG ATACACTGCT CCGCATTGAC 
TTGGGCAAGG CAAATGAGCC CAACCACGTG GAGCTGGGAC GTAAGGATGA CGCAAAAGTT 
CACAAGATGT TCCTTGACCA TACTGGCTCT CACCTGCTGA TTGCCCTGAG CAGCACGGAG 
GTCCTCTACG TGAACCGAAA TGGACAGAAG GTACGGCCAC TAGCACGCTG GAAGGGGCAG 
CTGGTGGAGA GTGTGGGTTG GAACAAGGCA CTGGGCACGG AGAGCAGCAC AGGCCCCATC 
CTGGTCGGGA CTGCCCAAGG CCACATCTTT GAAGCAGAGC TCTCAGCCAG CGAAGGTGGG 
CTTTTCGGCC CTGCTCCGGA TCTCTACTTC CGCCCATTGT ACGTGCTAAA TGAAGAAGGG 
GGTCCAGCAC CTGTGTGCTC CCTTGAGGCC GAGCGGGGCC CTGATGGGCG TAGCTTTGTT 
ATTGCCACCA CTCGGCAGCG CCTCTTCCAG TTCATAGGCC GAGCAGCAGA GGGGGCTGAG 
GCCCAGGGTT TCTCAGGGCT CTTTGCAGCT TACACGGACC ACCCACCCCC ATTCCGTGAG 
TTTCCCAGCA ACCTGGGCTA CAGTGAGTTG GCCTTCTACA CCCCCAAGCT GCGCTCCGCA 
CCCCGGGCCT TCGCCTGGAT GATGGGGGAT GGTGTGTTGT ATGGGGCATT GGACTGTGGG 
CGCCCTGACT CTCTGCTGAG CGAGGAGCGA GTCTGGGAGT ACCCAGAGGG GGTAGGGCCT 
GGGGCCAGCC CACCCCTAGC CATCGTCTTG ACCCAGTTCC ACTTCCTGCT GCTACTGGCA 
GACCGGGTGG AGGCAGTGTG CACACTGACC GGGCAGGTGG TGCTGCGGGA TCACTTCCTG 
GAGAAATTTG GGCCGCTGAA GCACATGGTG AAGGACTCCT CCACAGGCCA GCTGTGGGCC 
TACACTGAGC GGGCTGTCTT CCGCTACCAC GTGCAACGGG AGGCCCGAGA TGTCTGGCGC 
ACCTATCTGG ACATGAACCG CTTCGATCTG GCCAAAGAGT ATTGTCGAGA GCGGCCCGAC 
TGCCTGGACA CGGTCCTGGC CCGGGAGGCC GATTTCTGCT TTCGCCAGCG TCGCTACCTG 
GAGAGCGCAC GCTGCTATGC CCTGACCCAG AGCTACTTTG AGGAGATTGC CCTCAAGTTC 
CTGGAGGCCC GACAGGAGGA GGCTCTGGCT GAGTTCCTGC AGCGAAAACT GGCCAGTTTG 
AAGCCAGCCG AACGTACCCA GGCCACACTG CTGACCACCT GGCTGACAGA GCTCTACCTG 
AGCCGGCTTG GGGCTCTGCA GGGCGACCCA GAGGCCCTGA CTCTCTACCG AGAAACCAAG 
GAATGCTTTC GAACCTTCCT CAGCAGCCCC CGCCACAAAG AGTGGCTCTT TGCCAGCCGG 
GCCTCTATCC ATGAGCTGCT CGCCAGTCAT GGGGACACAG AACACATGGT GTACTTTGCA 
GTGATCATGC AGGACTATGA GCGGGTGGTG GCTTACCACT GTCAGCACGA GGCCTACGAG 
GAGGCCCTGG CCGTGCTCGC CCGCCACCGT GACCCCCAGC TCTTCTACAA GTTCTCACCC 
ATCCTCATCC GTCACATCCC CCGCCAGCTT GTAGATGCCT GGATTGAGAT GGGCAGCCGG 
CTGGATGCTC GTCAGCTCAT TCCTGCCCTG GTGAACTACA GCCAGGGTGG TGAGGTCCAG 
CAGGTGAGCC AGGCCATCCG CTACATGGAG TTCTGCGTGA ACGTGCTGGG GGAGACTGAG 
CAGGCCATCC ACAACTACCT GCTGTCACTG TATGCCCGTG GCCGGCCGGA CTCACTACTG 
GCCTATCTGG AGCAGGCTGG GGCCAGCCCC CACCGGGTGC ATTACGACCT CAAGTATGCG 
CTGCGGCTCT GCGCCGAGCA TGGCCACCAC CGCGCTTGTG TCCATGTCTA CAAGGTCCTA 
GAGCTGTATG AGGAGGCCGT GGACCTGGCC CTGCAGGTGG ATGTGGACCT GGCCAAGCAG 
TGTGCAGACC TGCCTGAGGA GGATGAGGAA TTGCGCAAGA AGCTGTGGCT GAAGATCGCA 
CGGCACGTGG TGCAGGAAGA GGAAGATGTA CAGACAGCCA TGGCTTGCCT GGCTAGCTGC 
CCCTTGCTCA AGATTGAGGA TGTGCTGCCC TTCTTTCCTG ATTTCGTCAC CATCGACCAC 
TTCAAGGAGG CGATCTGCAG CTCACTTAAG GCCTACAACC ACCACATCCA GGAGCTGCAG 
CGGGAGATGG AAGAGGCTAC AGCCAGTGCC CAGCGCATCC GGCGAGACCT GCAGGAGCTG 
CGGGGCCGCT ACGGCACTGT GGAGCCCCAG GACAAATGTG CCACCTGCGA CTTCCCCCTG 
CTCAACCGCC CTTTTTACCT CTTCCTCTGT GGCCATATGT TCCATGCTGA CTGCCTGCTG 
CAGGCTGTGC GACCTGGCCT GCCAGCCTAC AAGCAGGCCC GGCTGGAGGA GCTGCAGAGG 
AAGCTGGGGG CTGCTCCACC CCCAGCCAAG GGCTCTGCCC GGGCCAAGGA GGCCGAGGGT 
GGGGCTGCCA CGGCAGGGCC CAGCCGGGAA CAGCTCAAGG CTGACCTGGA TGAGTTGGTG 
GCCGCTGAGT GTGTGTACTG TGGGGAGCTG ATGATCCGCT CTATCGACCG GCCGTTCATC 
GACCCCCAGC GCTACGAGGA GGAGCAGCTC AGTTGGCTGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS18 ( XM_011521843.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520145.1 Homo sapiens VPS18 core subunit of CORVET and HOPS complexes (VPS18), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu70724
Clone ID Related Accession (Same CDS sequence)	XM_011521843.2
Accession Version	XM_011521843.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 18 homolog isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521843.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATATTT CAGGGAACAT TGACTTGGGC AAGGCAAATG AGCCCAACCA CGTGGAGCTG 
GGACGTAAGG ATGACGCAAA AGTTCACAAG ATGTTCCTTG ACCATACTGG CTCTCACCTG 
CTGATTGCCC TGAGCAGCAC GGAGGTCCTC TACGTGAACC GAAATGGACA GAAGGTACGG 
CCACTAGCAC GCTGGAAGGG GCAGCTGGTG GAGAGTGTGG GTTGGAACAA GGCACTGGGC 
ACGGAGAGCA GCACAGGCCC CATCCTGGTC GGGACTGCCC AAGGCCACAT CTTTGAAGCA 
GAGCTCTCAG CCAGCGAAGG TGGGCTTTTC GGCCCTGCTC CGGATCTCTA CTTCCGCCCA 
TTGTACGTGC TAAATGAAGA AGGGGGTCCA GCACCTGTGT GCTCCCTTGA GGCCGAGCGG 
GGCCCTGATG GGCGTAGCTT TGTTATTGCC ACCACTCGGC AGCGCCTCTT CCAGTTCATA 
GGCCGAGCAG CAGAGGGGGC TGAGGCCCAG GGTTTCTCAG GGCTCTTTGC AGCTTACACG 
GACCACCCAC CCCCATTCCG TGAGTTTCCC AGCAACCTGG GCTACAGTGA GTTGGCCTTC 
TACACCCCCA AGCTGCGCTC CGCACCCCGG GCCTTCGCCT GGATGATGGG GGATGGTGTG 
TTGTATGGGG CATTGGACTG TGGGCGCCCT GACTCTCTGC TGAGCGAGGA GCGAGTCTGG 
GAGTACCCAG AGGGGGTAGG GCCTGGGGCC AGCCCACCCC TAGCCATCGT CTTGACCCAG 
TTCCACTTCC TGCTGCTACT GGCAGACCGG GTGGAGGCAG TGTGCACACT GACCGGGCAG 
GTGGTGCTGC GGGATCACTT CCTGGAGAAA TTTGGGCCGC TGAAGCACAT GGTGAAGGAC 
TCCTCCACAG GCCAGCTGTG GGCCTACACT GAGCGGGCTG TCTTCCGCTA CCACGTGCAA 
CGGGAGGCCC GAGATGTCTG GCGCACCTAT CTGGACATGA ACCGCTTCGA TCTGGCCAAA 
GAGTATTGTC GAGAGCGGCC CGACTGCCTG GACACGGTCC TGGCCCGGGA GGCCGATTTC 
TGCTTTCGCC AGCGTCGCTA CCTGGAGAGC GCACGCTGCT ATGCCCTGAC CCAGAGCTAC 
TTTGAGGAGA TTGCCCTCAA GTTCCTGGAG GCCCGACAGG AGGAGGCTCT GGCTGAGTTC 
CTGCAGCGAA AACTGGCCAG TTTGAAGCCA GCCGAACGTA CCCAGGCCAC ACTGCTGACC 
ACCTGGCTGA CAGAGCTCTA CCTGAGCCGG CTTGGGGCTC TGCAGGGCGA CCCAGAGGCC 
CTGACTCTCT ACCGAGAAAC CAAGGAATGC TTTCGAACCT TCCTCAGCAG CCCCCGCCAC 
AAAGAGTGGC TCTTTGCCAG CCGGGCCTCT ATCCATGAGC TGCTCGCCAG TCATGGGGAC 
ACAGAACACA TGGTGTACTT TGCAGTGATC ATGCAGGACT ATGAGCGGGT GGTGGCTTAC 
CACTGTCAGC ACGAGGCCTA CGAGGAGGCC CTGGCCGTGC TCGCCCGCCA CCGTGACCCC 
CAGCTCTTCT ACAAGTTCTC ACCCATCCTC ATCCGTCACA TCCCCCGCCA GCTTGTAGAT 
GCCTGGATTG AGATGGGCAG CCGGCTGGAT GCTCGTCAGC TCATTCCTGC CCTGGTGAAC 
TACAGCCAGG GTGGTGAGGT CCAGCAGGTG AGCCAGGCCA TCCGCTACAT GGAGTTCTGC 
GTGAACGTGC TGGGGGAGAC TGAGCAGGCC ATCCACAACT ACCTGCTGTC ACTGTATGCC 
CGTGGCCGGC CGGACTCACT ACTGGCCTAT CTGGAGCAGG CTGGGGCCAG CCCCCACCGG 
GTGCATTACG ACCTCAAGTA TGCGCTGCGG CTCTGCGCCG AGCATGGCCA CCACCGCGCT 
TGTGTCCATG TCTACAAGGT CCTAGAGCTG TATGAGGAGG CCGTGGACCT GGCCCTGCAG 
GTGGATGTGG ACCTGGCCAA GCAGTGTGCA GACCTGCCTG AGGAGGATGA GGAATTGCGC 
AAGAAGCTGT GGCTGAAGAT CGCACGGCAC GTGGTGCAGG AAGAGGAAGA TGTACAGACA 
GCCATGGCTT GCCTGGCTAG CTGCCCCTTG CTCAAGATTG AGGATGTGCT GCCCTTCTTT 
CCTGATTTCG TCACCATCGA CCACTTCAAG GAGGCGATCT GCAGCTCACT TAAGGCCTAC 
AACCACCACA TCCAGGAGCT GCAGCGGGAG ATGGAAGAGG CTACAGCCAG TGCCCAGCGC 
ATCCGGCGAG ACCTGCAGGA GCTGCGGGGC CGCTACGGCA CTGTGGAGCC CCAGGACAAA 
TGTGCCACCT GCGACTTCCC CCTGCTCAAC CGCCCTTTTT ACCTCTTCCT CTGTGGCCAT 
ATGTTCCATG CTGACTGCCT GCTGCAGGCT GTGCGACCTG GCCTGCCAGC CTACAAGCAG 
GCCCGGCTGG AGGAGCTGCA GAGGAAGCTG GGGGCTGCTC CACCCCCAGC CAAGGGCTCT 
GCCCGGGCCA AGGAGGCCGA GGGTGGGGCT GCCACGGCAG GGCCCAGCCG GGAACAGCTC 
AAGGCTGACC TGGATGAGTT GGTGGCCGCT GAGTGTGTGT ACTGTGGGGA GCTGATGATC 
CGCTCTATCG ACCGGCCGTT CATCGACCCC CAGCGCTACG AGGAGGAGCA GCTCAGTTGG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520145.1
CDS	86..2791
Translation	MYISGNIDLGKANEPNHVELGRKDDAKVHKMFLDHTGSHLLIALSSTEVLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGHIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRSFVIATTRQRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSWL

Target ORF information:

RefSeq Version	XM_011521843.2
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS18 core subunit of CORVET and HOPS complexes (VPS18), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521843.2

ORF Insert Sequence:

ATGTATATTT CAGGGAACAT TGACTTGGGC AAGGCAAATG AGCCCAACCA CGTGGAGCTG 
GGACGTAAGG ATGACGCAAA AGTTCACAAG ATGTTCCTTG ACCATACTGG CTCTCACCTG 
CTGATTGCCC TGAGCAGCAC GGAGGTCCTC TACGTGAACC GAAATGGACA GAAGGTACGG 
CCACTAGCAC GCTGGAAGGG GCAGCTGGTG GAGAGTGTGG GTTGGAACAA GGCACTGGGC 
ACGGAGAGCA GCACAGGCCC CATCCTGGTC GGGACTGCCC AAGGCCACAT CTTTGAAGCA 
GAGCTCTCAG CCAGCGAAGG TGGGCTTTTC GGCCCTGCTC CGGATCTCTA CTTCCGCCCA 
TTGTACGTGC TAAATGAAGA AGGGGGTCCA GCACCTGTGT GCTCCCTTGA GGCCGAGCGG 
GGCCCTGATG GGCGTAGCTT TGTTATTGCC ACCACTCGGC AGCGCCTCTT CCAGTTCATA 
GGCCGAGCAG CAGAGGGGGC TGAGGCCCAG GGTTTCTCAG GGCTCTTTGC AGCTTACACG 
GACCACCCAC CCCCATTCCG TGAGTTTCCC AGCAACCTGG GCTACAGTGA GTTGGCCTTC 
TACACCCCCA AGCTGCGCTC CGCACCCCGG GCCTTCGCCT GGATGATGGG GGATGGTGTG 
TTGTATGGGG CATTGGACTG TGGGCGCCCT GACTCTCTGC TGAGCGAGGA GCGAGTCTGG 
GAGTACCCAG AGGGGGTAGG GCCTGGGGCC AGCCCACCCC TAGCCATCGT CTTGACCCAG 
TTCCACTTCC TGCTGCTACT GGCAGACCGG GTGGAGGCAG TGTGCACACT GACCGGGCAG 
GTGGTGCTGC GGGATCACTT CCTGGAGAAA TTTGGGCCGC TGAAGCACAT GGTGAAGGAC 
TCCTCCACAG GCCAGCTGTG GGCCTACACT GAGCGGGCTG TCTTCCGCTA CCACGTGCAA 
CGGGAGGCCC GAGATGTCTG GCGCACCTAT CTGGACATGA ACCGCTTCGA TCTGGCCAAA 
GAGTATTGTC GAGAGCGGCC CGACTGCCTG GACACGGTCC TGGCCCGGGA GGCCGATTTC 
TGCTTTCGCC AGCGTCGCTA CCTGGAGAGC GCACGCTGCT ATGCCCTGAC CCAGAGCTAC 
TTTGAGGAGA TTGCCCTCAA GTTCCTGGAG GCCCGACAGG AGGAGGCTCT GGCTGAGTTC 
CTGCAGCGAA AACTGGCCAG TTTGAAGCCA GCCGAACGTA CCCAGGCCAC ACTGCTGACC 
ACCTGGCTGA CAGAGCTCTA CCTGAGCCGG CTTGGGGCTC TGCAGGGCGA CCCAGAGGCC 
CTGACTCTCT ACCGAGAAAC CAAGGAATGC TTTCGAACCT TCCTCAGCAG CCCCCGCCAC 
AAAGAGTGGC TCTTTGCCAG CCGGGCCTCT ATCCATGAGC TGCTCGCCAG TCATGGGGAC 
ACAGAACACA TGGTGTACTT TGCAGTGATC ATGCAGGACT ATGAGCGGGT GGTGGCTTAC 
CACTGTCAGC ACGAGGCCTA CGAGGAGGCC CTGGCCGTGC TCGCCCGCCA CCGTGACCCC 
CAGCTCTTCT ACAAGTTCTC ACCCATCCTC ATCCGTCACA TCCCCCGCCA GCTTGTAGAT 
GCCTGGATTG AGATGGGCAG CCGGCTGGAT GCTCGTCAGC TCATTCCTGC CCTGGTGAAC 
TACAGCCAGG GTGGTGAGGT CCAGCAGGTG AGCCAGGCCA TCCGCTACAT GGAGTTCTGC 
GTGAACGTGC TGGGGGAGAC TGAGCAGGCC ATCCACAACT ACCTGCTGTC ACTGTATGCC 
CGTGGCCGGC CGGACTCACT ACTGGCCTAT CTGGAGCAGG CTGGGGCCAG CCCCCACCGG 
GTGCATTACG ACCTCAAGTA TGCGCTGCGG CTCTGCGCCG AGCATGGCCA CCACCGCGCT 
TGTGTCCATG TCTACAAGGT CCTAGAGCTG TATGAGGAGG CCGTGGACCT GGCCCTGCAG 
GTGGATGTGG ACCTGGCCAA GCAGTGTGCA GACCTGCCTG AGGAGGATGA GGAATTGCGC 
AAGAAGCTGT GGCTGAAGAT CGCACGGCAC GTGGTGCAGG AAGAGGAAGA TGTACAGACA 
GCCATGGCTT GCCTGGCTAG CTGCCCCTTG CTCAAGATTG AGGATGTGCT GCCCTTCTTT 
CCTGATTTCG TCACCATCGA CCACTTCAAG GAGGCGATCT GCAGCTCACT TAAGGCCTAC 
AACCACCACA TCCAGGAGCT GCAGCGGGAG ATGGAAGAGG CTACAGCCAG TGCCCAGCGC 
ATCCGGCGAG ACCTGCAGGA GCTGCGGGGC CGCTACGGCA CTGTGGAGCC CCAGGACAAA 
TGTGCCACCT GCGACTTCCC CCTGCTCAAC CGCCCTTTTT ACCTCTTCCT CTGTGGCCAT 
ATGTTCCATG CTGACTGCCT GCTGCAGGCT GTGCGACCTG GCCTGCCAGC CTACAAGCAG 
GCCCGGCTGG AGGAGCTGCA GAGGAAGCTG GGGGCTGCTC CACCCCCAGC CAAGGGCTCT 
GCCCGGGCCA AGGAGGCCGA GGGTGGGGCT GCCACGGCAG GGCCCAGCCG GGAACAGCTC 
AAGGCTGACC TGGATGAGTT GGTGGCCGCT GAGTGTGTGT ACTGTGGGGA GCTGATGATC 
CGCTCTATCG ACCGGCCGTT CATCGACCCC CAGCGCTACG AGGAGGAGCA GCTCAGTTGG 
CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS18 ( XM_011521844.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011520146.1 Homo sapiens VPS18 core subunit of CORVET and HOPS complexes (VPS18), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OHu70725
Clone ID Related Accession (Same CDS sequence)	XM_011521844.2
Accession Version	XM_011521844.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2616bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 18 homolog isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011521844.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTCCTTG ACCATACTGG CTCTCACCTG CTGATTGCCC TGAGCAGCAC GGAGGTCCTC 
TACGTGAACC GAAATGGACA GAAGGTACGG CCACTAGCAC GCTGGAAGGG GCAGCTGGTG 
GAGAGTGTGG GTTGGAACAA GGCACTGGGC ACGGAGAGCA GCACAGGCCC CATCCTGGTC 
GGGACTGCCC AAGGCCACAT CTTTGAAGCA GAGCTCTCAG CCAGCGAAGG TGGGCTTTTC 
GGCCCTGCTC CGGATCTCTA CTTCCGCCCA TTGTACGTGC TAAATGAAGA AGGGGGTCCA 
GCACCTGTGT GCTCCCTTGA GGCCGAGCGG GGCCCTGATG GGCGTAGCTT TGTTATTGCC 
ACCACTCGGC AGCGCCTCTT CCAGTTCATA GGCCGAGCAG CAGAGGGGGC TGAGGCCCAG 
GGTTTCTCAG GGCTCTTTGC AGCTTACACG GACCACCCAC CCCCATTCCG TGAGTTTCCC 
AGCAACCTGG GCTACAGTGA GTTGGCCTTC TACACCCCCA AGCTGCGCTC CGCACCCCGG 
GCCTTCGCCT GGATGATGGG GGATGGTGTG TTGTATGGGG CATTGGACTG TGGGCGCCCT 
GACTCTCTGC TGAGCGAGGA GCGAGTCTGG GAGTACCCAG AGGGGGTAGG GCCTGGGGCC 
AGCCCACCCC TAGCCATCGT CTTGACCCAG TTCCACTTCC TGCTGCTACT GGCAGACCGG 
GTGGAGGCAG TGTGCACACT GACCGGGCAG GTGGTGCTGC GGGATCACTT CCTGGAGAAA 
TTTGGGCCGC TGAAGCACAT GGTGAAGGAC TCCTCCACAG GCCAGCTGTG GGCCTACACT 
GAGCGGGCTG TCTTCCGCTA CCACGTGCAA CGGGAGGCCC GAGATGTCTG GCGCACCTAT 
CTGGACATGA ACCGCTTCGA TCTGGCCAAA GAGTATTGTC GAGAGCGGCC CGACTGCCTG 
GACACGGTCC TGGCCCGGGA GGCCGATTTC TGCTTTCGCC AGCGTCGCTA CCTGGAGAGC 
GCACGCTGCT ATGCCCTGAC CCAGAGCTAC TTTGAGGAGA TTGCCCTCAA GTTCCTGGAG 
GCCCGACAGG AGGAGGCTCT GGCTGAGTTC CTGCAGCGAA AACTGGCCAG TTTGAAGCCA 
GCCGAACGTA CCCAGGCCAC ACTGCTGACC ACCTGGCTGA CAGAGCTCTA CCTGAGCCGG 
CTTGGGGCTC TGCAGGGCGA CCCAGAGGCC CTGACTCTCT ACCGAGAAAC CAAGGAATGC 
TTTCGAACCT TCCTCAGCAG CCCCCGCCAC AAAGAGTGGC TCTTTGCCAG CCGGGCCTCT 
ATCCATGAGC TGCTCGCCAG TCATGGGGAC ACAGAACACA TGGTGTACTT TGCAGTGATC 
ATGCAGGACT ATGAGCGGGT GGTGGCTTAC CACTGTCAGC ACGAGGCCTA CGAGGAGGCC 
CTGGCCGTGC TCGCCCGCCA CCGTGACCCC CAGCTCTTCT ACAAGTTCTC ACCCATCCTC 
ATCCGTCACA TCCCCCGCCA GCTTGTAGAT GCCTGGATTG AGATGGGCAG CCGGCTGGAT 
GCTCGTCAGC TCATTCCTGC CCTGGTGAAC TACAGCCAGG GTGGTGAGGT CCAGCAGGTG 
AGCCAGGCCA TCCGCTACAT GGAGTTCTGC GTGAACGTGC TGGGGGAGAC TGAGCAGGCC 
ATCCACAACT ACCTGCTGTC ACTGTATGCC CGTGGCCGGC CGGACTCACT ACTGGCCTAT 
CTGGAGCAGG CTGGGGCCAG CCCCCACCGG GTGCATTACG ACCTCAAGTA TGCGCTGCGG 
CTCTGCGCCG AGCATGGCCA CCACCGCGCT TGTGTCCATG TCTACAAGGT CCTAGAGCTG 
TATGAGGAGG CCGTGGACCT GGCCCTGCAG GTGGATGTGG ACCTGGCCAA GCAGTGTGCA 
GACCTGCCTG AGGAGGATGA GGAATTGCGC AAGAAGCTGT GGCTGAAGAT CGCACGGCAC 
GTGGTGCAGG AAGAGGAAGA TGTACAGACA GCCATGGCTT GCCTGGCTAG CTGCCCCTTG 
CTCAAGATTG AGGATGTGCT GCCCTTCTTT CCTGATTTCG TCACCATCGA CCACTTCAAG 
GAGGCGATCT GCAGCTCACT TAAGGCCTAC AACCACCACA TCCAGGAGCT GCAGCGGGAG 
ATGGAAGAGG CTACAGCCAG TGCCCAGCGC ATCCGGCGAG ACCTGCAGGA GCTGCGGGGC 
CGCTACGGCA CTGTGGAGCC CCAGGACAAA TGTGCCACCT GCGACTTCCC CCTGCTCAAC 
CGCCCTTTTT ACCTCTTCCT CTGTGGCCAT ATGTTCCATG CTGACTGCCT GCTGCAGGCT 
GTGCGACCTG GCCTGCCAGC CTACAAGCAG GCCCGGCTGG AGGAGCTGCA GAGGAAGCTG 
GGGGCTGCTC CACCCCCAGC CAAGGGCTCT GCCCGGGCCA AGGAGGCCGA GGGTGGGGCT 
GCCACGGCAG GGCCCAGCCG GGAACAGCTC AAGGCTGACC TGGATGAGTT GGTGGCCGCT 
GAGTGTGTGT ACTGTGGGGA GCTGATGATC CGCTCTATCG ACCGGCCGTT CATCGACCCC 
CAGCGCTACG AGGAGGAGCA GCTCAGTTGG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011520146.1
CDS	751..3366
Translation	MFLDHTGSHLLIALSSTEVLYVNRNGQKVRPLARWKGQLVESVGWNKALGTESSTGPILVGTAQGHIFEAELSASEGGLFGPAPDLYFRPLYVLNEEGGPAPVCSLEAERGPDGRSFVIATTRQRLFQFIGRAAEGAEAQGFSGLFAAYTDHPPPFREFPSNLGYSELAFYTPKLRSAPRAFAWMMGDGVLYGALDCGRPDSLLSEERVWEYPEGVGPGASPPLAIVLTQFHFLLLLADRVEAVCTLTGQVVLRDHFLEKFGPLKHMVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSWL

Target ORF information:

RefSeq Version	XM_011521844.2
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS18 core subunit of CORVET and HOPS complexes (VPS18), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011521844.2

ORF Insert Sequence:

ATGTTCCTTG ACCATACTGG CTCTCACCTG CTGATTGCCC TGAGCAGCAC GGAGGTCCTC 
TACGTGAACC GAAATGGACA GAAGGTACGG CCACTAGCAC GCTGGAAGGG GCAGCTGGTG 
GAGAGTGTGG GTTGGAACAA GGCACTGGGC ACGGAGAGCA GCACAGGCCC CATCCTGGTC 
GGGACTGCCC AAGGCCACAT CTTTGAAGCA GAGCTCTCAG CCAGCGAAGG TGGGCTTTTC 
GGCCCTGCTC CGGATCTCTA CTTCCGCCCA TTGTACGTGC TAAATGAAGA AGGGGGTCCA 
GCACCTGTGT GCTCCCTTGA GGCCGAGCGG GGCCCTGATG GGCGTAGCTT TGTTATTGCC 
ACCACTCGGC AGCGCCTCTT CCAGTTCATA GGCCGAGCAG CAGAGGGGGC TGAGGCCCAG 
GGTTTCTCAG GGCTCTTTGC AGCTTACACG GACCACCCAC CCCCATTCCG TGAGTTTCCC 
AGCAACCTGG GCTACAGTGA GTTGGCCTTC TACACCCCCA AGCTGCGCTC CGCACCCCGG 
GCCTTCGCCT GGATGATGGG GGATGGTGTG TTGTATGGGG CATTGGACTG TGGGCGCCCT 
GACTCTCTGC TGAGCGAGGA GCGAGTCTGG GAGTACCCAG AGGGGGTAGG GCCTGGGGCC 
AGCCCACCCC TAGCCATCGT CTTGACCCAG TTCCACTTCC TGCTGCTACT GGCAGACCGG 
GTGGAGGCAG TGTGCACACT GACCGGGCAG GTGGTGCTGC GGGATCACTT CCTGGAGAAA 
TTTGGGCCGC TGAAGCACAT GGTGAAGGAC TCCTCCACAG GCCAGCTGTG GGCCTACACT 
GAGCGGGCTG TCTTCCGCTA CCACGTGCAA CGGGAGGCCC GAGATGTCTG GCGCACCTAT 
CTGGACATGA ACCGCTTCGA TCTGGCCAAA GAGTATTGTC GAGAGCGGCC CGACTGCCTG 
GACACGGTCC TGGCCCGGGA GGCCGATTTC TGCTTTCGCC AGCGTCGCTA CCTGGAGAGC 
GCACGCTGCT ATGCCCTGAC CCAGAGCTAC TTTGAGGAGA TTGCCCTCAA GTTCCTGGAG 
GCCCGACAGG AGGAGGCTCT GGCTGAGTTC CTGCAGCGAA AACTGGCCAG TTTGAAGCCA 
GCCGAACGTA CCCAGGCCAC ACTGCTGACC ACCTGGCTGA CAGAGCTCTA CCTGAGCCGG 
CTTGGGGCTC TGCAGGGCGA CCCAGAGGCC CTGACTCTCT ACCGAGAAAC CAAGGAATGC 
TTTCGAACCT TCCTCAGCAG CCCCCGCCAC AAAGAGTGGC TCTTTGCCAG CCGGGCCTCT 
ATCCATGAGC TGCTCGCCAG TCATGGGGAC ACAGAACACA TGGTGTACTT TGCAGTGATC 
ATGCAGGACT ATGAGCGGGT GGTGGCTTAC CACTGTCAGC ACGAGGCCTA CGAGGAGGCC 
CTGGCCGTGC TCGCCCGCCA CCGTGACCCC CAGCTCTTCT ACAAGTTCTC ACCCATCCTC 
ATCCGTCACA TCCCCCGCCA GCTTGTAGAT GCCTGGATTG AGATGGGCAG CCGGCTGGAT 
GCTCGTCAGC TCATTCCTGC CCTGGTGAAC TACAGCCAGG GTGGTGAGGT CCAGCAGGTG 
AGCCAGGCCA TCCGCTACAT GGAGTTCTGC GTGAACGTGC TGGGGGAGAC TGAGCAGGCC 
ATCCACAACT ACCTGCTGTC ACTGTATGCC CGTGGCCGGC CGGACTCACT ACTGGCCTAT 
CTGGAGCAGG CTGGGGCCAG CCCCCACCGG GTGCATTACG ACCTCAAGTA TGCGCTGCGG 
CTCTGCGCCG AGCATGGCCA CCACCGCGCT TGTGTCCATG TCTACAAGGT CCTAGAGCTG 
TATGAGGAGG CCGTGGACCT GGCCCTGCAG GTGGATGTGG ACCTGGCCAA GCAGTGTGCA 
GACCTGCCTG AGGAGGATGA GGAATTGCGC AAGAAGCTGT GGCTGAAGAT CGCACGGCAC 
GTGGTGCAGG AAGAGGAAGA TGTACAGACA GCCATGGCTT GCCTGGCTAG CTGCCCCTTG 
CTCAAGATTG AGGATGTGCT GCCCTTCTTT CCTGATTTCG TCACCATCGA CCACTTCAAG 
GAGGCGATCT GCAGCTCACT TAAGGCCTAC AACCACCACA TCCAGGAGCT GCAGCGGGAG 
ATGGAAGAGG CTACAGCCAG TGCCCAGCGC ATCCGGCGAG ACCTGCAGGA GCTGCGGGGC 
CGCTACGGCA CTGTGGAGCC CCAGGACAAA TGTGCCACCT GCGACTTCCC CCTGCTCAAC 
CGCCCTTTTT ACCTCTTCCT CTGTGGCCAT ATGTTCCATG CTGACTGCCT GCTGCAGGCT 
GTGCGACCTG GCCTGCCAGC CTACAAGCAG GCCCGGCTGG AGGAGCTGCA GAGGAAGCTG 
GGGGCTGCTC CACCCCCAGC CAAGGGCTCT GCCCGGGCCA AGGAGGCCGA GGGTGGGGCT 
GCCACGGCAG GGCCCAGCCG GGAACAGCTC AAGGCTGACC TGGATGAGTT GGTGGCCGCT 
GAGTGTGTGT ACTGTGGGGA GCTGATGATC CGCTCTATCG ACCGGCCGTT CATCGACCCC 
CAGCGCTACG AGGAGGAGCA GCTCAGTTGG CTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS18 ( XM_017022447.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_016877936.1 Homo sapiens VPS18 core subunit of CORVET and HOPS complexes (VPS18), transcript variant X3, mRNA.

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CloneID	OHu85578
Clone ID Related Accession (Same CDS sequence)	XM_017022447.1
Accession Version	XM_017022447.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 18 homolog isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000015.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGAAGG ACTCCTCCAC AGGCCAGCTG TGGGCCTACA CTGAGCGGGC TGTCTTCCGC 
TACCACGTGC AACGGGAGGC CCGAGATGTC TGGCGCACCT ATCTGGACAT GAACCGCTTC 
GATCTGGCCA AAGAGTATTG TCGAGAGCGG CCCGACTGCC TGGACACGGT CCTGGCCCGG 
GAGGCCGATT TCTGCTTTCG CCAGCGTCGC TACCTGGAGA GCGCACGCTG CTATGCCCTG 
ACCCAGAGCT ACTTTGAGGA GATTGCCCTC AAGTTCCTGG AGGCCCGACA GGAGGAGGCT 
CTGGCTGAGT TCCTGCAGCG AAAACTGGCC AGTTTGAAGC CAGCCGAACG TACCCAGGCC 
ACACTGCTGA CCACCTGGCT GACAGAGCTC TACCTGAGCC GGCTTGGGGC TCTGCAGGGC 
GACCCAGAGG CCCTGACTCT CTACCGAGAA ACCAAGGAAT GCTTTCGAAC CTTCCTCAGC 
AGCCCCCGCC ACAAAGAGTG GCTCTTTGCC AGCCGGGCCT CTATCCATGA GCTGCTCGCC 
AGTCATGGGG ACACAGAACA CATGGTGTAC TTTGCAGTGA TCATGCAGGA CTATGAGCGG 
GTGGTGGCTT ACCACTGTCA GCACGAGGCC TACGAGGAGG CCCTGGCCGT GCTCGCCCGC 
CACCGTGACC CCCAGCTCTT CTACAAGTTC TCACCCATCC TCATCCGTCA CATCCCCCGC 
CAGCTTGTAG ATGCCTGGAT TGAGATGGGC AGCCGGCTGG ATGCTCGTCA GCTCATTCCT 
GCCCTGGTGA ACTACAGCCA GGGTGGTGAG GTCCAGCAGG TGAGCCAGGC CATCCGCTAC 
ATGGAGTTCT GCGTGAACGT GCTGGGGGAG ACTGAGCAGG CCATCCACAA CTACCTGCTG 
TCACTGTATG CCCGTGGCCG GCCGGACTCA CTACTGGCCT ATCTGGAGCA GGCTGGGGCC 
AGCCCCCACC GGGTGCATTA CGACCTCAAG TATGCGCTGC GGCTCTGCGC CGAGCATGGC 
CACCACCGCG CTTGTGTCCA TGTCTACAAG GTCCTAGAGC TGTATGAGGA GGCCGTGGAC 
CTGGCCCTGC AGGTGGATGT GGACCTGGCC AAGCAGTGTG CAGACCTGCC TGAGGAGGAT 
GAGGAATTGC GCAAGAAGCT GTGGCTGAAG ATCGCACGGC ACGTGGTGCA GGAAGAGGAA 
GATGTACAGA CAGCCATGGC TTGCCTGGCT AGCTGCCCCT TGCTCAAGAT TGAGGATGTG 
CTGCCCTTCT TTCCTGATTT CGTCACCATC GACCACTTCA AGGAGGCGAT CTGCAGCTCA 
CTTAAGGCCT ACAACCACCA CATCCAGGAG CTGCAGCGGG AGATGGAAGA GGCTACAGCC 
AGTGCCCAGC GCATCCGGCG AGACCTGCAG GAGCTGCGGG GCCGCTACGG CACTGTGGAG 
CCCCAGGACA AATGTGCCAC CTGCGACTTC CCCCTGCTCA ACCGCCCTTT TTACCTCTTC 
CTCTGTGGCC ATATGTTCCA TGCTGACTGC CTGCTGCAGG CTGTGCGACC TGGCCTGCCA 
GCCTACAAGC AGGCCCGGCT GGAGGAGCTG CAGAGGAAGC TGGGGGCTGC TCCACCCCCA 
GCCAAGGGCT CTGCCCGGGC CAAGGAGGCC GAGGGTGGGG CTGCCACGGC AGGGCCCAGC 
CGGGAACAGC TCAAGGCTGA CCTGGATGAG TTGGTGGCCG CTGAGTGTGT GTACTGTGGG 
GAGCTGATGA TCCGCTCTAT CGACCGGCCG TTCATCGACC CCCAGCGCTA CGAGGAGGAG 
CAGCTCAGTT GGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016877936.1
CDS	833..2650
Translation	MVKDSSTGQLWAYTERAVFRYHVQREARDVWRTYLDMNRFDLAKEYCRERPDCLDTVLAREADFCFRQRRYLESARCYALTQSYFEEIALKFLEARQEEALAEFLQRKLASLKPAERTQATLLTTWLTELYLSRLGALQGDPEALTLYRETKECFRTFLSSPRHKEWLFASRASIHELLASHGDTEHMVYFAVIMQDYERVVAYHCQHEAYEEALAVLARHRDPQLFYKFSPILIRHIPRQLVDAWIEMGSRLDARQLIPALVNYSQGGEVQQVSQAIRYMEFCVNVLGETEQAIHNYLLSLYARGRPDSLLAYLEQAGASPHRVHYDLKYALRLCAEHGHHRACVHVYKVLELYEEAVDLALQVDVDLAKQCADLPEEDEELRKKLWLKIARHVVQEEEDVQTAMACLASCPLLKIEDVLPFFPDFVTIDHFKEAICSSLKAYNHHIQELQREMEEATASAQRIRRDLQELRGRYGTVEPQDKCATCDFPLLNRPFYLFLCGHMFHADCLLQAVRPGLPAYKQARLEELQRKLGAAPPPAKGSARAKEAEGGAATAGPSREQLKADLDELVAAECVYCGELMIRSIDRPFIDPQRYEEEQLSWL

Target ORF information:

RefSeq Version	XM_017022447.1
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS18 core subunit of CORVET and HOPS complexes (VPS18), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017022447.1

ORF Insert Sequence:

ATGGTGAAGG ACTCCTCCAC AGGCCAGCTG TGGGCCTACA CTGAGCGGGC TGTCTTCCGC 
TACCACGTGC AACGGGAGGC CCGAGATGTC TGGCGCACCT ATCTGGACAT GAACCGCTTC 
GATCTGGCCA AAGAGTATTG TCGAGAGCGG CCCGACTGCC TGGACACGGT CCTGGCCCGG 
GAGGCCGATT TCTGCTTTCG CCAGCGTCGC TACCTGGAGA GCGCACGCTG CTATGCCCTG 
ACCCAGAGCT ACTTTGAGGA GATTGCCCTC AAGTTCCTGG AGGCCCGACA GGAGGAGGCT 
CTGGCTGAGT TCCTGCAGCG AAAACTGGCC AGTTTGAAGC CAGCCGAACG TACCCAGGCC 
ACACTGCTGA CCACCTGGCT GACAGAGCTC TACCTGAGCC GGCTTGGGGC TCTGCAGGGC 
GACCCAGAGG CCCTGACTCT CTACCGAGAA ACCAAGGAAT GCTTTCGAAC CTTCCTCAGC 
AGCCCCCGCC ACAAAGAGTG GCTCTTTGCC AGCCGGGCCT CTATCCATGA GCTGCTCGCC 
AGTCATGGGG ACACAGAACA CATGGTGTAC TTTGCAGTGA TCATGCAGGA CTATGAGCGG 
GTGGTGGCTT ACCACTGTCA GCACGAGGCC TACGAGGAGG CCCTGGCCGT GCTCGCCCGC 
CACCGTGACC CCCAGCTCTT CTACAAGTTC TCACCCATCC TCATCCGTCA CATCCCCCGC 
CAGCTTGTAG ATGCCTGGAT TGAGATGGGC AGCCGGCTGG ATGCTCGTCA GCTCATTCCT 
GCCCTGGTGA ACTACAGCCA GGGTGGTGAG GTCCAGCAGG TGAGCCAGGC CATCCGCTAC 
ATGGAGTTCT GCGTGAACGT GCTGGGGGAG ACTGAGCAGG CCATCCACAA CTACCTGCTG 
TCACTGTATG CCCGTGGCCG GCCGGACTCA CTACTGGCCT ATCTGGAGCA GGCTGGGGCC 
AGCCCCCACC GGGTGCATTA CGACCTCAAG TATGCGCTGC GGCTCTGCGC CGAGCATGGC 
CACCACCGCG CTTGTGTCCA TGTCTACAAG GTCCTAGAGC TGTATGAGGA GGCCGTGGAC 
CTGGCCCTGC AGGTGGATGT GGACCTGGCC AAGCAGTGTG CAGACCTGCC TGAGGAGGAT 
GAGGAATTGC GCAAGAAGCT GTGGCTGAAG ATCGCACGGC ACGTGGTGCA GGAAGAGGAA 
GATGTACAGA CAGCCATGGC TTGCCTGGCT AGCTGCCCCT TGCTCAAGAT TGAGGATGTG 
CTGCCCTTCT TTCCTGATTT CGTCACCATC GACCACTTCA AGGAGGCGAT CTGCAGCTCA 
CTTAAGGCCT ACAACCACCA CATCCAGGAG CTGCAGCGGG AGATGGAAGA GGCTACAGCC 
AGTGCCCAGC GCATCCGGCG AGACCTGCAG GAGCTGCGGG GCCGCTACGG CACTGTGGAG 
CCCCAGGACA AATGTGCCAC CTGCGACTTC CCCCTGCTCA ACCGCCCTTT TTACCTCTTC 
CTCTGTGGCC ATATGTTCCA TGCTGACTGC CTGCTGCAGG CTGTGCGACC TGGCCTGCCA 
GCCTACAAGC AGGCCCGGCT GGAGGAGCTG CAGAGGAAGC TGGGGGCTGC TCCACCCCCA 
GCCAAGGGCT CTGCCCGGGC CAAGGAGGCC GAGGGTGGGG CTGCCACGGC AGGGCCCAGC 
CGGGAACAGC TCAAGGCTGA CCTGGATGAG TTGGTGGCCG CTGAGTGTGT GTACTGTGGG 
GAGCTGATGA TCCGCTCTAT CGACCGGCCG TTCATCGACC CCCAGCGCTA CGAGGAGGAG 
CAGCTCAGTT GGCTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction.
The Biochemical journal474(21)3615-3626(2017 10)
Hunter MR,Scourfield EJ,Emmott E,Graham SC

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Characterization of the Mammalian CORVET and HOPS Complexes and Their Modular Restructuring for Endosome Specificity.
The Journal of biological chemistry290(51)30280-90(2015 Dec)
van der Kant R,Jonker CT,Wijdeven RH,Bakker J,Janssen L,Klumperman J,Neefjes J

A human interactome in three quantitative dimensions organized by stoichiometries and abundances.
Cell163(3)712-23(2015 Oct)
Hein MY,Hubner NC,Poser I,Cox J,Nagaraj N,Toyoda Y,Gak IA,Weisswange I,Mansfeld J,Buchholz F,Hyman AA,Mann M

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VPS18 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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