NCEH1 cDNA ORF clone, Homo sapiens(human)

The following NCEH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NCEH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu14546 NM_001146277.3
Latest version!
Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$154.50-$216.30
$309.00
OHu14546 NM_001146278.2
Latest version!
Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$154.50-$216.30
$309.00
OHu14702 NM_001146276.1
Latest version!
Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$159.50-$223.30
$319.00
OHu14579 NM_020792.4
Latest version!
Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $265.30
$379.00
OHu107590 NM_001146276.3
Latest version!
Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu107607 NM_020792.6
Latest version!
Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
View Old Accession Versions >>
OHu107590 NM_001146276.2 Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu107607 NM_020792.5 Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $223.30
$319.00
OHu14546 NM_001146278.1 Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$154.50-$216.30
$309.00
OHu14546 NM_001146277.1 Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$154.50-$216.30
$309.00
OHu14546 NM_001146277.2 Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$154.50-$216.30
$309.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu14546
    Clone ID Related Accession (Same CDS sequence) NM_001146278.2 , NM_001146277.2 , NM_001146278.1 , NM_001146277.1 , NM_001146277.3
    Accession Version NM_001146278.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-20
    Organism Homo sapiens(human)
    Product neutral cholesterol ester hydrolase 1 isoform c
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA856205.1, AK295641.1, AC007919.18 and BP395319.1. On Sep 12, 2018 this sequence version replaced NM_001146278.1. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (c) is shorter than isoform a. Variants 3 and 4 encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.125250.1, SRR1803612.165048.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCTGAGG AATTGAATGC TGTCATTGTT TCCATTGAAT ACAGGCTAGT TCCAAAGGTT 
    TATTTTCCTG AGCAAATTCA TGATGTTGTA CGGGCCACAA AGTATTTCCT GAAGCCAGAA
    GTCTTACAGA AGTATATGGT TGATCCAGGC AGAATTTGCA TTTCTGGTGA CAGTGCTGGT
    GGAAATCTGG CTGCTGCCCT TGGACAACAG TTTACTCAAG ATGCCAGCCT AAAAAATAAG
    CTCAAACTAC AAGCTTTAAT TTATCCAGTT CTTCAAGCTT TAGATTTTAA CACACCATCT
    TATCAGCAAA ATGTGAACAC CCCAATCCTG CCCCGCTATG TCATGGTGAA GTATTGGGTG
    GACTACTTCA AAGGCAACTA TGACTTTGTG CAGGCAATGA TCGTTAACAA TCACACTTCA
    CTTGATGTGG AAGAGGCTGC TGCTGTCAGG GCCCGTCTAA ACTGGACATC CCTCTTGCCT
    GCATCCTTCA CAAAGAACTA CAAGCCTGTT GTACAGACCA CAGGCAATGC CAGGATTGTC
    CAGGAGCTTC CTCAGTTGCT GGATGCCCGC TCCGCCCCAC TCATTGCAGA CCAGGCAGTG
    CTGCAGCTCC TCCCAAAGAC CTACATTCTG ACGTGTGAGC ATGATGTCCT CAGAGACGAT
    GGCATCATGT ATGCCAAGCG TTTGGAGAGT GCCGGTGTGG AGGTGACCCT GGATCACTTT
    GAGGATGGCT TTCACGGATG TATGATTTTC ACTAGCTGGC CCACCAACTT CTCAGTGGGA
    ATCCGGACTA GGAATAGTTA CATCAAGTGG CTAGATCAAA ACCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139750.1
    CDS254..1081
    Translation

    Target ORF information:

    RefSeq Version NM_001146278.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001146278.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCTGAGG AATTGAATGC TGTCATTGTT TCCATTGAAT ACAGGCTAGT TCCAAAGGTT 
    TATTTTCCTG AGCAAATTCA TGATGTTGTA CGGGCCACAA AGTATTTCCT GAAGCCAGAA
    GTCTTACAGA AGTATATGGT TGATCCAGGC AGAATTTGCA TTTCTGGTGA CAGTGCTGGT
    GGAAATCTGG CTGCTGCCCT TGGACAACAG TTTACTCAAG ATGCCAGCCT AAAAAATAAG
    CTCAAACTAC AAGCTTTAAT TTATCCAGTT CTTCAAGCTT TAGATTTTAA CACACCATCT
    TATCAGCAAA ATGTGAACAC CCCAATCCTG CCCCGCTATG TCATGGTGAA GTATTGGGTG
    GACTACTTCA AAGGCAACTA TGACTTTGTG CAGGCAATGA TCGTTAACAA TCACACTTCA
    CTTGATGTGG AAGAGGCTGC TGCTGTCAGG GCCCGTCTAA ACTGGACATC CCTCTTGCCT
    GCATCCTTCA CAAAGAACTA CAAGCCTGTT GTACAGACCA CAGGCAATGC CAGGATTGTC
    CAGGAGCTTC CTCAGTTGCT GGATGCCCGC TCCGCCCCAC TCATTGCAGA CCAGGCAGTG
    CTGCAGCTCC TCCCAAAGAC CTACATTCTG ACGTGTGAGC ATGATGTCCT CAGAGACGAT
    GGCATCATGT ATGCCAAGCG TTTGGAGAGT GCCGGTGTGG AGGTGACCCT GGATCACTTT
    GAGGATGGCT TTCACGGATG TATGATTTTC ACTAGCTGGC CCACCAACTT CTCAGTGGGA
    ATCCGGACTA GGAATAGTTA CATCAAGTGG CTAGATCAAA ACCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14546
    Clone ID Related Accession (Same CDS sequence) NM_001146278.2 , NM_001146277.2 , NM_001146278.1 , NM_001146277.1 , NM_001146277.3
    Accession Version NM_001146277.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product neutral cholesterol ester hydrolase 1 isoform c
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC306836.1, BC028734.1, AC007919.18 and BP395319.1. On Sep 12, 2018 this sequence version replaced NM_001146277.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (c) is shorter than isoform a. Variants 3 and 4 encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.57040.1, SRR1803616.201150.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCTGAGG AATTGAATGC TGTCATTGTT TCCATTGAAT ACAGGCTAGT TCCAAAGGTT 
    TATTTTCCTG AGCAAATTCA TGATGTTGTA CGGGCCACAA AGTATTTCCT GAAGCCAGAA
    GTCTTACAGA AGTATATGGT TGATCCAGGC AGAATTTGCA TTTCTGGTGA CAGTGCTGGT
    GGAAATCTGG CTGCTGCCCT TGGACAACAG TTTACTCAAG ATGCCAGCCT AAAAAATAAG
    CTCAAACTAC AAGCTTTAAT TTATCCAGTT CTTCAAGCTT TAGATTTTAA CACACCATCT
    TATCAGCAAA ATGTGAACAC CCCAATCCTG CCCCGCTATG TCATGGTGAA GTATTGGGTG
    GACTACTTCA AAGGCAACTA TGACTTTGTG CAGGCAATGA TCGTTAACAA TCACACTTCA
    CTTGATGTGG AAGAGGCTGC TGCTGTCAGG GCCCGTCTAA ACTGGACATC CCTCTTGCCT
    GCATCCTTCA CAAAGAACTA CAAGCCTGTT GTACAGACCA CAGGCAATGC CAGGATTGTC
    CAGGAGCTTC CTCAGTTGCT GGATGCCCGC TCCGCCCCAC TCATTGCAGA CCAGGCAGTG
    CTGCAGCTCC TCCCAAAGAC CTACATTCTG ACGTGTGAGC ATGATGTCCT CAGAGACGAT
    GGCATCATGT ATGCCAAGCG TTTGGAGAGT GCCGGTGTGG AGGTGACCCT GGATCACTTT
    GAGGATGGCT TTCACGGATG TATGATTTTC ACTAGCTGGC CCACCAACTT CTCAGTGGGA
    ATCCGGACTA GGAATAGTTA CATCAAGTGG CTAGATCAAA ACCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139749.1
    CDS473..1300
    Translation

    Target ORF information:

    RefSeq Version NM_001146277.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001146277.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCTGAGG AATTGAATGC TGTCATTGTT TCCATTGAAT ACAGGCTAGT TCCAAAGGTT 
    TATTTTCCTG AGCAAATTCA TGATGTTGTA CGGGCCACAA AGTATTTCCT GAAGCCAGAA
    GTCTTACAGA AGTATATGGT TGATCCAGGC AGAATTTGCA TTTCTGGTGA CAGTGCTGGT
    GGAAATCTGG CTGCTGCCCT TGGACAACAG TTTACTCAAG ATGCCAGCCT AAAAAATAAG
    CTCAAACTAC AAGCTTTAAT TTATCCAGTT CTTCAAGCTT TAGATTTTAA CACACCATCT
    TATCAGCAAA ATGTGAACAC CCCAATCCTG CCCCGCTATG TCATGGTGAA GTATTGGGTG
    GACTACTTCA AAGGCAACTA TGACTTTGTG CAGGCAATGA TCGTTAACAA TCACACTTCA
    CTTGATGTGG AAGAGGCTGC TGCTGTCAGG GCCCGTCTAA ACTGGACATC CCTCTTGCCT
    GCATCCTTCA CAAAGAACTA CAAGCCTGTT GTACAGACCA CAGGCAATGC CAGGATTGTC
    CAGGAGCTTC CTCAGTTGCT GGATGCCCGC TCCGCCCCAC TCATTGCAGA CCAGGCAGTG
    CTGCAGCTCC TCCCAAAGAC CTACATTCTG ACGTGTGAGC ATGATGTCCT CAGAGACGAT
    GGCATCATGT ATGCCAAGCG TTTGGAGAGT GCCGGTGTGG AGGTGACCCT GGATCACTTT
    GAGGATGGCT TTCACGGATG TATGATTTTC ACTAGCTGGC CCACCAACTT CTCAGTGGGA
    ATCCGGACTA GGAATAGTTA CATCAAGTGG CTAGATCAAA ACCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14546
    Clone ID Related Accession (Same CDS sequence) NM_001146278.2 , NM_001146277.2 , NM_001146278.1 , NM_001146277.1 , NM_001146277.3
    Accession Version NM_001146278.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product neutral cholesterol ester hydrolase 1 isoform c
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB289027.1, DA856205.1, AK295641.1, AC007919.18 and BP395319.1. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (c) is shorter than isoform a. Variants 3 and 4 encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.275662.1, SRR1660807.110728.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCTGAGG AATTGAATGC TGTCATTGTT TCCATTGAAT ACAGGCTAGT TCCAAAGGTT 
    TATTTTCCTG AGCAAATTCA TGATGTTGTA CGGGCCACAA AGTATTTCCT GAAGCCAGAA
    GTCTTACAGA AGTATATGGT TGATCCAGGC AGAATTTGCA TTTCTGGTGA CAGTGCTGGT
    GGAAATCTGG CTGCTGCCCT TGGACAACAG TTTACTCAAG ATGCCAGCCT AAAAAATAAG
    CTCAAACTAC AAGCTTTAAT TTATCCAGTT CTTCAAGCTT TAGATTTTAA CACACCATCT
    TATCAGCAAA ATGTGAACAC CCCAATCCTG CCCCGCTATG TCATGGTGAA GTATTGGGTG
    GACTACTTCA AAGGCAACTA TGACTTTGTG CAGGCAATGA TCGTTAACAA TCACACTTCA
    CTTGATGTGG AAGAGGCTGC TGCTGTCAGG GCCCGTCTAA ACTGGACATC CCTCTTGCCT
    GCATCCTTCA CAAAGAACTA CAAGCCTGTT GTACAGACCA CAGGCAATGC CAGGATTGTC
    CAGGAGCTTC CTCAGTTGCT GGATGCCCGC TCCGCCCCAC TCATTGCAGA CCAGGCAGTG
    CTGCAGCTCC TCCCAAAGAC CTACATTCTG ACGTGTGAGC ATGATGTCCT CAGAGACGAT
    GGCATCATGT ATGCCAAGCG TTTGGAGAGT GCCGGTGTGG AGGTGACCCT GGATCACTTT
    GAGGATGGCT TTCACGGATG TATGATTTTC ACTAGCTGGC CCACCAACTT CTCAGTGGGA
    ATCCGGACTA GGAATAGTTA CATCAAGTGG CTAGATCAAA ACCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139750.1
    CDS405..1232
    Translation

    Target ORF information:

    RefSeq Version NM_001146278.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001146278.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCTGAGG AATTGAATGC TGTCATTGTT TCCATTGAAT ACAGGCTAGT TCCAAAGGTT 
    TATTTTCCTG AGCAAATTCA TGATGTTGTA CGGGCCACAA AGTATTTCCT GAAGCCAGAA
    GTCTTACAGA AGTATATGGT TGATCCAGGC AGAATTTGCA TTTCTGGTGA CAGTGCTGGT
    GGAAATCTGG CTGCTGCCCT TGGACAACAG TTTACTCAAG ATGCCAGCCT AAAAAATAAG
    CTCAAACTAC AAGCTTTAAT TTATCCAGTT CTTCAAGCTT TAGATTTTAA CACACCATCT
    TATCAGCAAA ATGTGAACAC CCCAATCCTG CCCCGCTATG TCATGGTGAA GTATTGGGTG
    GACTACTTCA AAGGCAACTA TGACTTTGTG CAGGCAATGA TCGTTAACAA TCACACTTCA
    CTTGATGTGG AAGAGGCTGC TGCTGTCAGG GCCCGTCTAA ACTGGACATC CCTCTTGCCT
    GCATCCTTCA CAAAGAACTA CAAGCCTGTT GTACAGACCA CAGGCAATGC CAGGATTGTC
    CAGGAGCTTC CTCAGTTGCT GGATGCCCGC TCCGCCCCAC TCATTGCAGA CCAGGCAGTG
    CTGCAGCTCC TCCCAAAGAC CTACATTCTG ACGTGTGAGC ATGATGTCCT CAGAGACGAT
    GGCATCATGT ATGCCAAGCG TTTGGAGAGT GCCGGTGTGG AGGTGACCCT GGATCACTTT
    GAGGATGGCT TTCACGGATG TATGATTTTC ACTAGCTGGC CCACCAACTT CTCAGTGGGA
    ATCCGGACTA GGAATAGTTA CATCAAGTGG CTAGATCAAA ACCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14546
    Clone ID Related Accession (Same CDS sequence) NM_001146278.2 , NM_001146277.2 , NM_001146278.1 , NM_001146277.1 , NM_001146277.3
    Accession Version NM_001146277.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product neutral cholesterol ester hydrolase 1 isoform c
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB289027.1, BG701753.1, DC306836.1, BC028734.1, AC007919.18 and BP395319.1. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (c) is shorter than isoform a. Variants 3 and 4 encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803616.201150.1, SRR1660803.238811.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCTGAGG AATTGAATGC TGTCATTGTT TCCATTGAAT ACAGGCTAGT TCCAAAGGTT 
    TATTTTCCTG AGCAAATTCA TGATGTTGTA CGGGCCACAA AGTATTTCCT GAAGCCAGAA
    GTCTTACAGA AGTATATGGT TGATCCAGGC AGAATTTGCA TTTCTGGTGA CAGTGCTGGT
    GGAAATCTGG CTGCTGCCCT TGGACAACAG TTTACTCAAG ATGCCAGCCT AAAAAATAAG
    CTCAAACTAC AAGCTTTAAT TTATCCAGTT CTTCAAGCTT TAGATTTTAA CACACCATCT
    TATCAGCAAA ATGTGAACAC CCCAATCCTG CCCCGCTATG TCATGGTGAA GTATTGGGTG
    GACTACTTCA AAGGCAACTA TGACTTTGTG CAGGCAATGA TCGTTAACAA TCACACTTCA
    CTTGATGTGG AAGAGGCTGC TGCTGTCAGG GCCCGTCTAA ACTGGACATC CCTCTTGCCT
    GCATCCTTCA CAAAGAACTA CAAGCCTGTT GTACAGACCA CAGGCAATGC CAGGATTGTC
    CAGGAGCTTC CTCAGTTGCT GGATGCCCGC TCCGCCCCAC TCATTGCAGA CCAGGCAGTG
    CTGCAGCTCC TCCCAAAGAC CTACATTCTG ACGTGTGAGC ATGATGTCCT CAGAGACGAT
    GGCATCATGT ATGCCAAGCG TTTGGAGAGT GCCGGTGTGG AGGTGACCCT GGATCACTTT
    GAGGATGGCT TTCACGGATG TATGATTTTC ACTAGCTGGC CCACCAACTT CTCAGTGGGA
    ATCCGGACTA GGAATAGTTA CATCAAGTGG CTAGATCAAA ACCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139749.1
    CDS624..1451
    Translation

    Target ORF information:

    RefSeq Version NM_001146277.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001146277.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCTGAGG AATTGAATGC TGTCATTGTT TCCATTGAAT ACAGGCTAGT TCCAAAGGTT 
    TATTTTCCTG AGCAAATTCA TGATGTTGTA CGGGCCACAA AGTATTTCCT GAAGCCAGAA
    GTCTTACAGA AGTATATGGT TGATCCAGGC AGAATTTGCA TTTCTGGTGA CAGTGCTGGT
    GGAAATCTGG CTGCTGCCCT TGGACAACAG TTTACTCAAG ATGCCAGCCT AAAAAATAAG
    CTCAAACTAC AAGCTTTAAT TTATCCAGTT CTTCAAGCTT TAGATTTTAA CACACCATCT
    TATCAGCAAA ATGTGAACAC CCCAATCCTG CCCCGCTATG TCATGGTGAA GTATTGGGTG
    GACTACTTCA AAGGCAACTA TGACTTTGTG CAGGCAATGA TCGTTAACAA TCACACTTCA
    CTTGATGTGG AAGAGGCTGC TGCTGTCAGG GCCCGTCTAA ACTGGACATC CCTCTTGCCT
    GCATCCTTCA CAAAGAACTA CAAGCCTGTT GTACAGACCA CAGGCAATGC CAGGATTGTC
    CAGGAGCTTC CTCAGTTGCT GGATGCCCGC TCCGCCCCAC TCATTGCAGA CCAGGCAGTG
    CTGCAGCTCC TCCCAAAGAC CTACATTCTG ACGTGTGAGC ATGATGTCCT CAGAGACGAT
    GGCATCATGT ATGCCAAGCG TTTGGAGAGT GCCGGTGTGG AGGTGACCCT GGATCACTTT
    GAGGATGGCT TTCACGGATG TATGATTTTC ACTAGCTGGC CCACCAACTT CTCAGTGGGA
    ATCCGGACTA GGAATAGTTA CATCAAGTGG CTAGATCAAA ACCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14546
    Clone ID Related Accession (Same CDS sequence) NM_001146278.2 , NM_001146277.2 , NM_001146278.1 , NM_001146277.1 , NM_001146277.3
    Accession Version NM_001146277.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 828bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-20
    Organism Homo sapiens(human)
    Product neutral cholesterol ester hydrolase 1 isoform c
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DC306836.1, BC028734.1, AC007919.18 and BP395319.1. On Jun 2, 2019 this sequence version replaced NM_001146277.2. Transcript Variant: This variant (3) differs in the 5' UTR, lacks a portion of the 5' coding region, and initiates translation at a downstream start codon compared to variant 1. The encoded isoform (c) is shorter than isoform a. Variants 3 and 4 encode the same isoform (c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.57040.1, SRR1660803.238811.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCTGAGG AATTGAATGC TGTCATTGTT TCCATTGAAT ACAGGCTAGT TCCAAAGGTT 
    TATTTTCCTG AGCAAATTCA TGATGTTGTA CGGGCCACAA AGTATTTCCT GAAGCCAGAA
    GTCTTACAGA AGTATATGGT TGATCCAGGC AGAATTTGCA TTTCTGGTGA CAGTGCTGGT
    GGAAATCTGG CTGCTGCCCT TGGACAACAG TTTACTCAAG ATGCCAGCCT AAAAAATAAG
    CTCAAACTAC AAGCTTTAAT TTATCCAGTT CTTCAAGCTT TAGATTTTAA CACACCATCT
    TATCAGCAAA ATGTGAACAC CCCAATCCTG CCCCGCTATG TCATGGTGAA GTATTGGGTG
    GACTACTTCA AAGGCAACTA TGACTTTGTG CAGGCAATGA TCGTTAACAA TCACACTTCA
    CTTGATGTGG AAGAGGCTGC TGCTGTCAGG GCCCGTCTAA ACTGGACATC CCTCTTGCCT
    GCATCCTTCA CAAAGAACTA CAAGCCTGTT GTACAGACCA CAGGCAATGC CAGGATTGTC
    CAGGAGCTTC CTCAGTTGCT GGATGCCCGC TCCGCCCCAC TCATTGCAGA CCAGGCAGTG
    CTGCAGCTCC TCCCAAAGAC CTACATTCTG ACGTGTGAGC ATGATGTCCT CAGAGACGAT
    GGCATCATGT ATGCCAAGCG TTTGGAGAGT GCCGGTGTGG AGGTGACCCT GGATCACTTT
    GAGGATGGCT TTCACGGATG TATGATTTTC ACTAGCTGGC CCACCAACTT CTCAGTGGGA
    ATCCGGACTA GGAATAGTTA CATCAAGTGG CTAGATCAAA ACCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139749.1
    CDS473..1300
    Translation

    Target ORF information:

    RefSeq Version NM_001146277.3
    Organism Homo sapiens(human)
    Definition Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001146277.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    ATGGCTGAGG AATTGAATGC TGTCATTGTT TCCATTGAAT ACAGGCTAGT TCCAAAGGTT 
    TATTTTCCTG AGCAAATTCA TGATGTTGTA CGGGCCACAA AGTATTTCCT GAAGCCAGAA
    GTCTTACAGA AGTATATGGT TGATCCAGGC AGAATTTGCA TTTCTGGTGA CAGTGCTGGT
    GGAAATCTGG CTGCTGCCCT TGGACAACAG TTTACTCAAG ATGCCAGCCT AAAAAATAAG
    CTCAAACTAC AAGCTTTAAT TTATCCAGTT CTTCAAGCTT TAGATTTTAA CACACCATCT
    TATCAGCAAA ATGTGAACAC CCCAATCCTG CCCCGCTATG TCATGGTGAA GTATTGGGTG
    GACTACTTCA AAGGCAACTA TGACTTTGTG CAGGCAATGA TCGTTAACAA TCACACTTCA
    CTTGATGTGG AAGAGGCTGC TGCTGTCAGG GCCCGTCTAA ACTGGACATC CCTCTTGCCT
    GCATCCTTCA CAAAGAACTA CAAGCCTGTT GTACAGACCA CAGGCAATGC CAGGATTGTC
    CAGGAGCTTC CTCAGTTGCT GGATGCCCGC TCCGCCCCAC TCATTGCAGA CCAGGCAGTG
    CTGCAGCTCC TCCCAAAGAC CTACATTCTG ACGTGTGAGC ATGATGTCCT CAGAGACGAT
    GGCATCATGT ATGCCAAGCG TTTGGAGAGT GCCGGTGTGG AGGTGACCCT GGATCACTTT
    GAGGATGGCT TTCACGGATG TATGATTTTC ACTAGCTGGC CCACCAACTT CTCAGTGGGA
    ATCCGGACTA GGAATAGTTA CATCAAGTGG CTAGATCAAA ACCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14702
    Clone ID Related Accession (Same CDS sequence) NM_001146276.1
    Accession Version NM_001146276.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product neutral cholesterol ester hydrolase 1 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB289027.1, DA856205.1, AK304253.1, BC028734.1, AC007919.18 and BP395319.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.249382.1, SRR1803617.118435.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGCAGCT GCCGCGGGCA GAAAGTTGCC GGAGGTCTCC GGGTGGTATC GCCCTTTCCT 
    CTTTGCCAGC CCGCTGGCGA GCCGAGCCAG GGCAAGATGA GGTCGTCCTG TGTCCTGCTC
    ACCGCCCTGG TGGCGCTGGC CGCCTATTAC GTCTACATCC CGCTGCCTGG CTCCGTGTCC
    GACCCCTGGA AGCTGATGCT GCTGGACGCC ACTTTCCGGG GTGCACAGCA AGTGAGTAAC
    CTGATCCACT ACCTGGGACT GAGCCATCAC CTGCTGGCAC TGAATTTTAT CATTGTTTCT
    TTTGGCAAAA AAAGCGCGTG GTCTTCTGCC CAAGTGAAGG TGACCGACAC AGACTTTGAT
    GGTGTGGAAG TCAGAGTGTT TGAAGGCCCT CCGAAGCCCG AAGAGCCACT GAAACGCAGC
    GTCGTTTATA TCCACGGAGG AGGCTGGGCC TTGGCAAGTG CAAGTGCGTC CTGGTCACCT
    TCAGATGAAA TCAGGTATTA TGATGAGCTG TGTACAGCAA TGGCTGAGGA ATTGAATGCT
    GTCATTGTTT CCATTGAATA CAGGCTAGTT CCAAAGGTTT ATTTTCCTGA GCAAATTCAT
    GATGTTGTAC GGGCCACAAA GTATTTCCTG AAGCCAGAAG TCTTACAGAA GTATATGGTT
    GATCCAGGCA GAATTTGCAT TTCTGGTGAC AGTGCTGGTG GAAATCTGGC TGCTGCCCTT
    GGACAACAGT TTACTCAAGA TGCCAGCCTA AAAAATAAGC TCAAACTACA AGCTTTAATT
    TATCCAGTTC TTCAAGCTTT AGATTTTAAC ACACCATCTT ATCAGCAAAA TGTGAACACC
    CCAATCCTGC CCCGCTATGT CATGGTGAAG TATTGGGTGG ACTACTTCAA AGGCAACTAT
    GACTTTGTGC AGGCAATGAT CGTTAACAAT CACACTTCAC TTGATGTGGA AGAGGCTGCT
    GCTGTCAGGG CCCGTCTAAA CTGGACATCC CTCTTGCCTG CATCCTTCAC AAAGAACTAC
    AAGCCTGTTG TACAGACCAC AGGCAATGCC AGGATTGTCC AGGAGCTTCC TCAGTTGCTG
    GATGCCCGCT CCGCCCCACT CATTGCAGAC CAGGCAGTGC TGCAGCTCCT CCCAAAGACC
    TACATTCTGA CGTGTGAGCA TGATGTCCTC AGAGACGATG GCATCATGTA TGCCAAGCGT
    TTGGAGAGTG CCGGTGTGGA GGTGACCCTG GATCACTTTG AGGATGGCTT TCACGGATGT
    ATGATTTTCA CTAGCTGGCC CACCAACTTC TCAGTGGGAA TCCGGACTAG GAATAGTTAC
    ATCAAGTGGC TAGATCAAAA CCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139748.1
    CDS139..1485
    Translation

    Target ORF information:

    RefSeq Version NM_001146276.1
    Organism Homo sapiens(human)
    Definition Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001146276.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGCAGCT GCCGCGGGCA GAAAGTTGCC GGAGGTCTCC GGGTGGTATC GCCCTTTCCT 
    CTTTGCCAGC CCGCTGGCGA GCCGAGCCAG GGCAAGATGA GGTCGTCCTG TGTCCTGCTC
    ACCGCCCTGG TGGCGCTGGC CGCCTATTAC GTCTACATCC CGCTGCCTGG CTCCGTGTCC
    GACCCCTGGA AGCTGATGCT GCTGGACGCC ACTTTCCGGG GTGCACAGCA AGTGAGTAAC
    CTGATCCACT ACCTGGGACT GAGCCATCAC CTGCTGGCAC TGAATTTTAT CATTGTTTCT
    TTTGGCAAAA AAAGCGCGTG GTCTTCTGCC CAAGTGAAGG TGACCGACAC AGACTTTGAT
    GGTGTGGAAG TCAGAGTGTT TGAAGGCCCT CCGAAGCCCG AAGAGCCACT GAAACGCAGC
    GTCGTTTATA TCCACGGAGG AGGCTGGGCC TTGGCAAGTG CAAGTGCGTC CTGGTCACCT
    TCAGATGAAA TCAGGTATTA TGATGAGCTG TGTACAGCAA TGGCTGAGGA ATTGAATGCT
    GTCATTGTTT CCATTGAATA CAGGCTAGTT CCAAAGGTTT ATTTTCCTGA GCAAATTCAT
    GATGTTGTAC GGGCCACAAA GTATTTCCTG AAGCCAGAAG TCTTACAGAA GTATATGGTT
    GATCCAGGCA GAATTTGCAT TTCTGGTGAC AGTGCTGGTG GAAATCTGGC TGCTGCCCTT
    GGACAACAGT TTACTCAAGA TGCCAGCCTA AAAAATAAGC TCAAACTACA AGCTTTAATT
    TATCCAGTTC TTCAAGCTTT AGATTTTAAC ACACCATCTT ATCAGCAAAA TGTGAACACC
    CCAATCCTGC CCCGCTATGT CATGGTGAAG TATTGGGTGG ACTACTTCAA AGGCAACTAT
    GACTTTGTGC AGGCAATGAT CGTTAACAAT CACACTTCAC TTGATGTGGA AGAGGCTGCT
    GCTGTCAGGG CCCGTCTAAA CTGGACATCC CTCTTGCCTG CATCCTTCAC AAAGAACTAC
    AAGCCTGTTG TACAGACCAC AGGCAATGCC AGGATTGTCC AGGAGCTTCC TCAGTTGCTG
    GATGCCCGCT CCGCCCCACT CATTGCAGAC CAGGCAGTGC TGCAGCTCCT CCCAAAGACC
    TACATTCTGA CGTGTGAGCA TGATGTCCTC AGAGACGATG GCATCATGTA TGCCAAGCGT
    TTGGAGAGTG CCGGTGTGGA GGTGACCCTG GATCACTTTG AGGATGGCTT TCACGGATGT
    ATGATTTTCA CTAGCTGGCC CACCAACTTC TCAGTGGGAA TCCGGACTAG GAATAGTTAC
    ATCAAGTGGC TAGATCAAAA CCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu14579
    Clone ID Related Accession (Same CDS sequence) NM_020792.4
    Accession Version NM_020792.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1323bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-01
    Organism Homo sapiens(human)
    Product neutral cholesterol ester hydrolase 1 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DB289027.1, DA856205.1, BC028734.1, AC007919.18 and BP395319.1. On Apr 7, 2009 this sequence version replaced NM_020792.3. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform b) compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: This CCDS representation uses the 5'-most in-frame start codon, which is conserved in higher primates. An alternative downstream start codon, which is more widely conserved and has a stronger Kozak signal, also exists. It is possible that leaky scanning by ribosomes would allow the downstream start codon to be used, at least some of the time. The use of the downstream start codon would result in a protein that is 32 aa shorter at the N-terminus. There is no experimental evidence showing which start codon is preferentially used in vivo. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028734.1, SRR1803612.85240.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGCAGCT GCCGCGGGCA GAAAGTTGCC GGAGGTCTCC GGGTGGTATC GCCCTTTCCT 
    CTTTGCCAGC CCGCTGGCGA GCCGAGCCAG GGCAAGATGA GGTCGTCCTG TGTCCTGCTC
    ACCGCCCTGG TGGCGCTGGC CGCCTATTAC GTCTACATCC CGCTGCCTGG CTCCGTGTCC
    GACCCCTGGA AGCTGATGCT GCTGGACGCC ACTTTCCGGG GTGCACAGCA AGTGAGTAAC
    CTGATCCACT ACCTGGGACT GAGCCATCAC CTGCTGGCAC TGAATTTTAT CATTGTTTCT
    TTTGGCAAAA AAAGCGCGTG GTCTTCTGCC CAAGTGAAGG TGACCGACAC AGACTTTGAT
    GGTGTGGAAG TCAGAGTGTT TGAAGGCCCT CCGAAGCCCG AAGAGCCACT GAAACGCAGC
    GTCGTTTATA TCCACGGAGG AGGCTGGGCC TTGGCAAGTG CAAAAATCAG GTATTATGAT
    GAGCTGTGTA CAGCAATGGC TGAGGAATTG AATGCTGTCA TTGTTTCCAT TGAATACAGG
    CTAGTTCCAA AGGTTTATTT TCCTGAGCAA ATTCATGATG TTGTACGGGC CACAAAGTAT
    TTCCTGAAGC CAGAAGTCTT ACAGAAGTAT ATGGTTGATC CAGGCAGAAT TTGCATTTCT
    GGTGACAGTG CTGGTGGAAA TCTGGCTGCT GCCCTTGGAC AACAGTTTAC TCAAGATGCC
    AGCCTAAAAA ATAAGCTCAA ACTACAAGCT TTAATTTATC CAGTTCTTCA AGCTTTAGAT
    TTTAACACAC CATCTTATCA GCAAAATGTG AACACCCCAA TCCTGCCCCG CTATGTCATG
    GTGAAGTATT GGGTGGACTA CTTCAAAGGC AACTATGACT TTGTGCAGGC AATGATCGTT
    AACAATCACA CTTCACTTGA TGTGGAAGAG GCTGCTGCTG TCAGGGCCCG TCTAAACTGG
    ACATCCCTCT TGCCTGCATC CTTCACAAAG AACTACAAGC CTGTTGTACA GACCACAGGC
    AATGCCAGGA TTGTCCAGGA GCTTCCTCAG TTGCTGGATG CCCGCTCCGC CCCACTCATT
    GCAGACCAGG CAGTGCTGCA GCTCCTCCCA AAGACCTACA TTCTGACGTG TGAGCATGAT
    GTCCTCAGAG ACGATGGCAT CATGTATGCC AAGCGTTTGG AGAGTGCCGG TGTGGAGGTG
    ACCCTGGATC ACTTTGAGGA TGGCTTTCAC GGATGTATGA TTTTCACTAG CTGGCCCACC
    AACTTCTCAG TGGGAATCCG GACTAGGAAT AGTTACATCA AGTGGCTAGA TCAAAACCTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065843.3
    CDS139..1461
    Translation

    Target ORF information:

    RefSeq Version NM_020792.4
    Organism Homo sapiens(human)
    Definition Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020792.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGAGCAGCT GCCGCGGGCA GAAAGTTGCC GGAGGTCTCC GGGTGGTATC GCCCTTTCCT 
    CTTTGCCAGC CCGCTGGCGA GCCGAGCCAG GGCAAGATGA GGTCGTCCTG TGTCCTGCTC
    ACCGCCCTGG TGGCGCTGGC CGCCTATTAC GTCTACATCC CGCTGCCTGG CTCCGTGTCC
    GACCCCTGGA AGCTGATGCT GCTGGACGCC ACTTTCCGGG GTGCACAGCA AGTGAGTAAC
    CTGATCCACT ACCTGGGACT GAGCCATCAC CTGCTGGCAC TGAATTTTAT CATTGTTTCT
    TTTGGCAAAA AAAGCGCGTG GTCTTCTGCC CAAGTGAAGG TGACCGACAC AGACTTTGAT
    GGTGTGGAAG TCAGAGTGTT TGAAGGCCCT CCGAAGCCCG AAGAGCCACT GAAACGCAGC
    GTCGTTTATA TCCACGGAGG AGGCTGGGCC TTGGCAAGTG CAAAAATCAG GTATTATGAT
    GAGCTGTGTA CAGCAATGGC TGAGGAATTG AATGCTGTCA TTGTTTCCAT TGAATACAGG
    CTAGTTCCAA AGGTTTATTT TCCTGAGCAA ATTCATGATG TTGTACGGGC CACAAAGTAT
    TTCCTGAAGC CAGAAGTCTT ACAGAAGTAT ATGGTTGATC CAGGCAGAAT TTGCATTTCT
    GGTGACAGTG CTGGTGGAAA TCTGGCTGCT GCCCTTGGAC AACAGTTTAC TCAAGATGCC
    AGCCTAAAAA ATAAGCTCAA ACTACAAGCT TTAATTTATC CAGTTCTTCA AGCTTTAGAT
    TTTAACACAC CATCTTATCA GCAAAATGTG AACACCCCAA TCCTGCCCCG CTATGTCATG
    GTGAAGTATT GGGTGGACTA CTTCAAAGGC AACTATGACT TTGTGCAGGC AATGATCGTT
    AACAATCACA CTTCACTTGA TGTGGAAGAG GCTGCTGCTG TCAGGGCCCG TCTAAACTGG
    ACATCCCTCT TGCCTGCATC CTTCACAAAG AACTACAAGC CTGTTGTACA GACCACAGGC
    AATGCCAGGA TTGTCCAGGA GCTTCCTCAG TTGCTGGATG CCCGCTCCGC CCCACTCATT
    GCAGACCAGG CAGTGCTGCA GCTCCTCCCA AAGACCTACA TTCTGACGTG TGAGCATGAT
    GTCCTCAGAG ACGATGGCAT CATGTATGCC AAGCGTTTGG AGAGTGCCGG TGTGGAGGTG
    ACCCTGGATC ACTTTGAGGA TGGCTTTCAC GGATGTATGA TTTTCACTAG CTGGCCCACC
    AACTTCTCAG TGGGAATCCG GACTAGGAAT AGTTACATCA AGTGGCTAGA TCAAAACCTG
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107590
    Clone ID Related Accession (Same CDS sequence) NM_001146276.3 , NM_001146276.2
    Accession Version NM_001146276.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-20
    Organism Homo sapiens(human)
    Product neutral cholesterol ester hydrolase 1 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA856205.1, AK304253.1, BC028734.1, AC007919.18 and BP395319.1. On Jun 2, 2019 this sequence version replaced NM_001146276.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by available conservation data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803614.216033.1, SRR1803612.204459.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAGGTCGT CCTGTGTCCT GCTCACCGCC CTGGTGGCGC TGGCCGCCTA TTACGTCTAC 
    ATCCCGCTGC CTGGCTCCGT GTCCGACCCC TGGAAGCTGA TGCTGCTGGA CGCCACTTTC
    CGGGGTGCAC AGCAAGTGAG TAACCTGATC CACTACCTGG GACTGAGCCA TCACCTGCTG
    GCACTGAATT TTATCATTGT TTCTTTTGGC AAAAAAAGCG CGTGGTCTTC TGCCCAAGTG
    AAGGTGACCG ACACAGACTT TGATGGTGTG GAAGTCAGAG TGTTTGAAGG CCCTCCGAAG
    CCCGAAGAGC CACTGAAACG CAGCGTCGTT TATATCCACG GAGGAGGCTG GGCCTTGGCA
    AGTGCAAGTG CGTCCTGGTC ACCTTCAGAT GAAATCAGGT ATTATGATGA GCTGTGTACA
    GCAATGGCTG AGGAATTGAA TGCTGTCATT GTTTCCATTG AATACAGGCT AGTTCCAAAG
    GTTTATTTTC CTGAGCAAAT TCATGATGTT GTACGGGCCA CAAAGTATTT CCTGAAGCCA
    GAAGTCTTAC AGAAGTATAT GGTTGATCCA GGCAGAATTT GCATTTCTGG TGACAGTGCT
    GGTGGAAATC TGGCTGCTGC CCTTGGACAA CAGTTTACTC AAGATGCCAG CCTAAAAAAT
    AAGCTCAAAC TACAAGCTTT AATTTATCCA GTTCTTCAAG CTTTAGATTT TAACACACCA
    TCTTATCAGC AAAATGTGAA CACCCCAATC CTGCCCCGCT ATGTCATGGT GAAGTATTGG
    GTGGACTACT TCAAAGGCAA CTATGACTTT GTGCAGGCAA TGATCGTTAA CAATCACACT
    TCACTTGATG TGGAAGAGGC TGCTGCTGTC AGGGCCCGTC TAAACTGGAC ATCCCTCTTG
    CCTGCATCCT TCACAAAGAA CTACAAGCCT GTTGTACAGA CCACAGGCAA TGCCAGGATT
    GTCCAGGAGC TTCCTCAGTT GCTGGATGCC CGCTCCGCCC CACTCATTGC AGACCAGGCA
    GTGCTGCAGC TCCTCCCAAA GACCTACATT CTGACGTGTG AGCATGATGT CCTCAGAGAC
    GATGGCATCA TGTATGCCAA GCGTTTGGAG AGTGCCGGTG TGGAGGTGAC CCTGGATCAC
    TTTGAGGATG GCTTTCACGG ATGTATGATT TTCACTAGCT GGCCCACCAA CTTCTCAGTG
    GGAATCCGGA CTAGGAATAG TTACATCAAG TGGCTAGATC AAAACCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139748.2
    CDS84..1334
    Translation

    Target ORF information:

    RefSeq Version NM_001146276.3
    Organism Homo sapiens(human)
    Definition Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001146276.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAGGTCGT CCTGTGTCCT GCTCACCGCC CTGGTGGCGC TGGCCGCCTA TTACGTCTAC 
    ATCCCGCTGC CTGGCTCCGT GTCCGACCCC TGGAAGCTGA TGCTGCTGGA CGCCACTTTC
    CGGGGTGCAC AGCAAGTGAG TAACCTGATC CACTACCTGG GACTGAGCCA TCACCTGCTG
    GCACTGAATT TTATCATTGT TTCTTTTGGC AAAAAAAGCG CGTGGTCTTC TGCCCAAGTG
    AAGGTGACCG ACACAGACTT TGATGGTGTG GAAGTCAGAG TGTTTGAAGG CCCTCCGAAG
    CCCGAAGAGC CACTGAAACG CAGCGTCGTT TATATCCACG GAGGAGGCTG GGCCTTGGCA
    AGTGCAAGTG CGTCCTGGTC ACCTTCAGAT GAAATCAGGT ATTATGATGA GCTGTGTACA
    GCAATGGCTG AGGAATTGAA TGCTGTCATT GTTTCCATTG AATACAGGCT AGTTCCAAAG
    GTTTATTTTC CTGAGCAAAT TCATGATGTT GTACGGGCCA CAAAGTATTT CCTGAAGCCA
    GAAGTCTTAC AGAAGTATAT GGTTGATCCA GGCAGAATTT GCATTTCTGG TGACAGTGCT
    GGTGGAAATC TGGCTGCTGC CCTTGGACAA CAGTTTACTC AAGATGCCAG CCTAAAAAAT
    AAGCTCAAAC TACAAGCTTT AATTTATCCA GTTCTTCAAG CTTTAGATTT TAACACACCA
    TCTTATCAGC AAAATGTGAA CACCCCAATC CTGCCCCGCT ATGTCATGGT GAAGTATTGG
    GTGGACTACT TCAAAGGCAA CTATGACTTT GTGCAGGCAA TGATCGTTAA CAATCACACT
    TCACTTGATG TGGAAGAGGC TGCTGCTGTC AGGGCCCGTC TAAACTGGAC ATCCCTCTTG
    CCTGCATCCT TCACAAAGAA CTACAAGCCT GTTGTACAGA CCACAGGCAA TGCCAGGATT
    GTCCAGGAGC TTCCTCAGTT GCTGGATGCC CGCTCCGCCC CACTCATTGC AGACCAGGCA
    GTGCTGCAGC TCCTCCCAAA GACCTACATT CTGACGTGTG AGCATGATGT CCTCAGAGAC
    GATGGCATCA TGTATGCCAA GCGTTTGGAG AGTGCCGGTG TGGAGGTGAC CCTGGATCAC
    TTTGAGGATG GCTTTCACGG ATGTATGATT TTCACTAGCT GGCCCACCAA CTTCTCAGTG
    GGAATCCGGA CTAGGAATAG TTACATCAAG TGGCTAGATC AAAACCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107590
    Clone ID Related Accession (Same CDS sequence) NM_001146276.3 , NM_001146276.2
    Accession Version NM_001146276.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1251bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-12-22
    Organism Homo sapiens(human)
    Product neutral cholesterol ester hydrolase 1 isoform a
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA856205.1, AK304253.1, BC028734.1, AC007919.18 and BP395319.1. On Sep 12, 2018 this sequence version replaced NM_001146276.1. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by available conservation data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803615.249382.1, SRR1803617.118435.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAGGTCGT CCTGTGTCCT GCTCACCGCC CTGGTGGCGC TGGCCGCCTA TTACGTCTAC 
    ATCCCGCTGC CTGGCTCCGT GTCCGACCCC TGGAAGCTGA TGCTGCTGGA CGCCACTTTC
    CGGGGTGCAC AGCAAGTGAG TAACCTGATC CACTACCTGG GACTGAGCCA TCACCTGCTG
    GCACTGAATT TTATCATTGT TTCTTTTGGC AAAAAAAGCG CGTGGTCTTC TGCCCAAGTG
    AAGGTGACCG ACACAGACTT TGATGGTGTG GAAGTCAGAG TGTTTGAAGG CCCTCCGAAG
    CCCGAAGAGC CACTGAAACG CAGCGTCGTT TATATCCACG GAGGAGGCTG GGCCTTGGCA
    AGTGCAAGTG CGTCCTGGTC ACCTTCAGAT GAAATCAGGT ATTATGATGA GCTGTGTACA
    GCAATGGCTG AGGAATTGAA TGCTGTCATT GTTTCCATTG AATACAGGCT AGTTCCAAAG
    GTTTATTTTC CTGAGCAAAT TCATGATGTT GTACGGGCCA CAAAGTATTT CCTGAAGCCA
    GAAGTCTTAC AGAAGTATAT GGTTGATCCA GGCAGAATTT GCATTTCTGG TGACAGTGCT
    GGTGGAAATC TGGCTGCTGC CCTTGGACAA CAGTTTACTC AAGATGCCAG CCTAAAAAAT
    AAGCTCAAAC TACAAGCTTT AATTTATCCA GTTCTTCAAG CTTTAGATTT TAACACACCA
    TCTTATCAGC AAAATGTGAA CACCCCAATC CTGCCCCGCT ATGTCATGGT GAAGTATTGG
    GTGGACTACT TCAAAGGCAA CTATGACTTT GTGCAGGCAA TGATCGTTAA CAATCACACT
    TCACTTGATG TGGAAGAGGC TGCTGCTGTC AGGGCCCGTC TAAACTGGAC ATCCCTCTTG
    CCTGCATCCT TCACAAAGAA CTACAAGCCT GTTGTACAGA CCACAGGCAA TGCCAGGATT
    GTCCAGGAGC TTCCTCAGTT GCTGGATGCC CGCTCCGCCC CACTCATTGC AGACCAGGCA
    GTGCTGCAGC TCCTCCCAAA GACCTACATT CTGACGTGTG AGCATGATGT CCTCAGAGAC
    GATGGCATCA TGTATGCCAA GCGTTTGGAG AGTGCCGGTG TGGAGGTGAC CCTGGATCAC
    TTTGAGGATG GCTTTCACGG ATGTATGATT TTCACTAGCT GGCCCACCAA CTTCTCAGTG
    GGAATCCGGA CTAGGAATAG TTACATCAAG TGGCTAGATC AAAACCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001139748.2
    CDS84..1334
    Translation

    Target ORF information:

    RefSeq Version NM_001146276.2
    Organism Homo sapiens(human)
    Definition Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001146276.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAGGTCGT CCTGTGTCCT GCTCACCGCC CTGGTGGCGC TGGCCGCCTA TTACGTCTAC 
    ATCCCGCTGC CTGGCTCCGT GTCCGACCCC TGGAAGCTGA TGCTGCTGGA CGCCACTTTC
    CGGGGTGCAC AGCAAGTGAG TAACCTGATC CACTACCTGG GACTGAGCCA TCACCTGCTG
    GCACTGAATT TTATCATTGT TTCTTTTGGC AAAAAAAGCG CGTGGTCTTC TGCCCAAGTG
    AAGGTGACCG ACACAGACTT TGATGGTGTG GAAGTCAGAG TGTTTGAAGG CCCTCCGAAG
    CCCGAAGAGC CACTGAAACG CAGCGTCGTT TATATCCACG GAGGAGGCTG GGCCTTGGCA
    AGTGCAAGTG CGTCCTGGTC ACCTTCAGAT GAAATCAGGT ATTATGATGA GCTGTGTACA
    GCAATGGCTG AGGAATTGAA TGCTGTCATT GTTTCCATTG AATACAGGCT AGTTCCAAAG
    GTTTATTTTC CTGAGCAAAT TCATGATGTT GTACGGGCCA CAAAGTATTT CCTGAAGCCA
    GAAGTCTTAC AGAAGTATAT GGTTGATCCA GGCAGAATTT GCATTTCTGG TGACAGTGCT
    GGTGGAAATC TGGCTGCTGC CCTTGGACAA CAGTTTACTC AAGATGCCAG CCTAAAAAAT
    AAGCTCAAAC TACAAGCTTT AATTTATCCA GTTCTTCAAG CTTTAGATTT TAACACACCA
    TCTTATCAGC AAAATGTGAA CACCCCAATC CTGCCCCGCT ATGTCATGGT GAAGTATTGG
    GTGGACTACT TCAAAGGCAA CTATGACTTT GTGCAGGCAA TGATCGTTAA CAATCACACT
    TCACTTGATG TGGAAGAGGC TGCTGCTGTC AGGGCCCGTC TAAACTGGAC ATCCCTCTTG
    CCTGCATCCT TCACAAAGAA CTACAAGCCT GTTGTACAGA CCACAGGCAA TGCCAGGATT
    GTCCAGGAGC TTCCTCAGTT GCTGGATGCC CGCTCCGCCC CACTCATTGC AGACCAGGCA
    GTGCTGCAGC TCCTCCCAAA GACCTACATT CTGACGTGTG AGCATGATGT CCTCAGAGAC
    GATGGCATCA TGTATGCCAA GCGTTTGGAG AGTGCCGGTG TGGAGGTGAC CCTGGATCAC
    TTTGAGGATG GCTTTCACGG ATGTATGATT TTCACTAGCT GGCCCACCAA CTTCTCAGTG
    GGAATCCGGA CTAGGAATAG TTACATCAAG TGGCTAGATC AAAACCTGTA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107607
    Clone ID Related Accession (Same CDS sequence) NM_020792.6 , NM_020792.5
    Accession Version NM_020792.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product neutral cholesterol ester hydrolase 1 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA856205.1, BC028734.1, AC007919.18 and BP395319.1. On Jun 2, 2019 this sequence version replaced NM_020792.5. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform b) compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by available conservation data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.147216.1, SRR1803615.191983.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAGGTCGT CCTGTGTCCT GCTCACCGCC CTGGTGGCGC TGGCCGCCTA TTACGTCTAC 
    ATCCCGCTGC CTGGCTCCGT GTCCGACCCC TGGAAGCTGA TGCTGCTGGA CGCCACTTTC
    CGGGGTGCAC AGCAAGTGAG TAACCTGATC CACTACCTGG GACTGAGCCA TCACCTGCTG
    GCACTGAATT TTATCATTGT TTCTTTTGGC AAAAAAAGCG CGTGGTCTTC TGCCCAAGTG
    AAGGTGACCG ACACAGACTT TGATGGTGTG GAAGTCAGAG TGTTTGAAGG CCCTCCGAAG
    CCCGAAGAGC CACTGAAACG CAGCGTCGTT TATATCCACG GAGGAGGCTG GGCCTTGGCA
    AGTGCAAAAA TCAGGTATTA TGATGAGCTG TGTACAGCAA TGGCTGAGGA ATTGAATGCT
    GTCATTGTTT CCATTGAATA CAGGCTAGTT CCAAAGGTTT ATTTTCCTGA GCAAATTCAT
    GATGTTGTAC GGGCCACAAA GTATTTCCTG AAGCCAGAAG TCTTACAGAA GTATATGGTT
    GATCCAGGCA GAATTTGCAT TTCTGGTGAC AGTGCTGGTG GAAATCTGGC TGCTGCCCTT
    GGACAACAGT TTACTCAAGA TGCCAGCCTA AAAAATAAGC TCAAACTACA AGCTTTAATT
    TATCCAGTTC TTCAAGCTTT AGATTTTAAC ACACCATCTT ATCAGCAAAA TGTGAACACC
    CCAATCCTGC CCCGCTATGT CATGGTGAAG TATTGGGTGG ACTACTTCAA AGGCAACTAT
    GACTTTGTGC AGGCAATGAT CGTTAACAAT CACACTTCAC TTGATGTGGA AGAGGCTGCT
    GCTGTCAGGG CCCGTCTAAA CTGGACATCC CTCTTGCCTG CATCCTTCAC AAAGAACTAC
    AAGCCTGTTG TACAGACCAC AGGCAATGCC AGGATTGTCC AGGAGCTTCC TCAGTTGCTG
    GATGCCCGCT CCGCCCCACT CATTGCAGAC CAGGCAGTGC TGCAGCTCCT CCCAAAGACC
    TACATTCTGA CGTGTGAGCA TGATGTCCTC AGAGACGATG GCATCATGTA TGCCAAGCGT
    TTGGAGAGTG CCGGTGTGGA GGTGACCCTG GATCACTTTG AGGATGGCTT TCACGGATGT
    ATGATTTTCA CTAGCTGGCC CACCAACTTC TCAGTGGGAA TCCGGACTAG GAATAGTTAC
    ATCAAGTGGC TAGATCAAAA CCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065843.4
    CDS84..1310
    Translation

    Target ORF information:

    RefSeq Version NM_020792.6
    Organism Homo sapiens(human)
    Definition Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020792.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAGGTCGT CCTGTGTCCT GCTCACCGCC CTGGTGGCGC TGGCCGCCTA TTACGTCTAC 
    ATCCCGCTGC CTGGCTCCGT GTCCGACCCC TGGAAGCTGA TGCTGCTGGA CGCCACTTTC
    CGGGGTGCAC AGCAAGTGAG TAACCTGATC CACTACCTGG GACTGAGCCA TCACCTGCTG
    GCACTGAATT TTATCATTGT TTCTTTTGGC AAAAAAAGCG CGTGGTCTTC TGCCCAAGTG
    AAGGTGACCG ACACAGACTT TGATGGTGTG GAAGTCAGAG TGTTTGAAGG CCCTCCGAAG
    CCCGAAGAGC CACTGAAACG CAGCGTCGTT TATATCCACG GAGGAGGCTG GGCCTTGGCA
    AGTGCAAAAA TCAGGTATTA TGATGAGCTG TGTACAGCAA TGGCTGAGGA ATTGAATGCT
    GTCATTGTTT CCATTGAATA CAGGCTAGTT CCAAAGGTTT ATTTTCCTGA GCAAATTCAT
    GATGTTGTAC GGGCCACAAA GTATTTCCTG AAGCCAGAAG TCTTACAGAA GTATATGGTT
    GATCCAGGCA GAATTTGCAT TTCTGGTGAC AGTGCTGGTG GAAATCTGGC TGCTGCCCTT
    GGACAACAGT TTACTCAAGA TGCCAGCCTA AAAAATAAGC TCAAACTACA AGCTTTAATT
    TATCCAGTTC TTCAAGCTTT AGATTTTAAC ACACCATCTT ATCAGCAAAA TGTGAACACC
    CCAATCCTGC CCCGCTATGT CATGGTGAAG TATTGGGTGG ACTACTTCAA AGGCAACTAT
    GACTTTGTGC AGGCAATGAT CGTTAACAAT CACACTTCAC TTGATGTGGA AGAGGCTGCT
    GCTGTCAGGG CCCGTCTAAA CTGGACATCC CTCTTGCCTG CATCCTTCAC AAAGAACTAC
    AAGCCTGTTG TACAGACCAC AGGCAATGCC AGGATTGTCC AGGAGCTTCC TCAGTTGCTG
    GATGCCCGCT CCGCCCCACT CATTGCAGAC CAGGCAGTGC TGCAGCTCCT CCCAAAGACC
    TACATTCTGA CGTGTGAGCA TGATGTCCTC AGAGACGATG GCATCATGTA TGCCAAGCGT
    TTGGAGAGTG CCGGTGTGGA GGTGACCCTG GATCACTTTG AGGATGGCTT TCACGGATGT
    ATGATTTTCA CTAGCTGGCC CACCAACTTC TCAGTGGGAA TCCGGACTAG GAATAGTTAC
    ATCAAGTGGC TAGATCAAAA CCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu107607
    Clone ID Related Accession (Same CDS sequence) NM_020792.6 , NM_020792.5
    Accession Version NM_020792.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1227bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-26
    Organism Homo sapiens(human)
    Product neutral cholesterol ester hydrolase 1 isoform b
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DA856205.1, BC028734.1, AC007919.18 and BP395319.1. On Sep 13, 2018 this sequence version replaced NM_020792.4. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform b) compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by available conservation data. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC028734.1, SRR1803615.191983.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAGGTCGT CCTGTGTCCT GCTCACCGCC CTGGTGGCGC TGGCCGCCTA TTACGTCTAC 
    ATCCCGCTGC CTGGCTCCGT GTCCGACCCC TGGAAGCTGA TGCTGCTGGA CGCCACTTTC
    CGGGGTGCAC AGCAAGTGAG TAACCTGATC CACTACCTGG GACTGAGCCA TCACCTGCTG
    GCACTGAATT TTATCATTGT TTCTTTTGGC AAAAAAAGCG CGTGGTCTTC TGCCCAAGTG
    AAGGTGACCG ACACAGACTT TGATGGTGTG GAAGTCAGAG TGTTTGAAGG CCCTCCGAAG
    CCCGAAGAGC CACTGAAACG CAGCGTCGTT TATATCCACG GAGGAGGCTG GGCCTTGGCA
    AGTGCAAAAA TCAGGTATTA TGATGAGCTG TGTACAGCAA TGGCTGAGGA ATTGAATGCT
    GTCATTGTTT CCATTGAATA CAGGCTAGTT CCAAAGGTTT ATTTTCCTGA GCAAATTCAT
    GATGTTGTAC GGGCCACAAA GTATTTCCTG AAGCCAGAAG TCTTACAGAA GTATATGGTT
    GATCCAGGCA GAATTTGCAT TTCTGGTGAC AGTGCTGGTG GAAATCTGGC TGCTGCCCTT
    GGACAACAGT TTACTCAAGA TGCCAGCCTA AAAAATAAGC TCAAACTACA AGCTTTAATT
    TATCCAGTTC TTCAAGCTTT AGATTTTAAC ACACCATCTT ATCAGCAAAA TGTGAACACC
    CCAATCCTGC CCCGCTATGT CATGGTGAAG TATTGGGTGG ACTACTTCAA AGGCAACTAT
    GACTTTGTGC AGGCAATGAT CGTTAACAAT CACACTTCAC TTGATGTGGA AGAGGCTGCT
    GCTGTCAGGG CCCGTCTAAA CTGGACATCC CTCTTGCCTG CATCCTTCAC AAAGAACTAC
    AAGCCTGTTG TACAGACCAC AGGCAATGCC AGGATTGTCC AGGAGCTTCC TCAGTTGCTG
    GATGCCCGCT CCGCCCCACT CATTGCAGAC CAGGCAGTGC TGCAGCTCCT CCCAAAGACC
    TACATTCTGA CGTGTGAGCA TGATGTCCTC AGAGACGATG GCATCATGTA TGCCAAGCGT
    TTGGAGAGTG CCGGTGTGGA GGTGACCCTG GATCACTTTG AGGATGGCTT TCACGGATGT
    ATGATTTTCA CTAGCTGGCC CACCAACTTC TCAGTGGGAA TCCGGACTAG GAATAGTTAC
    ATCAAGTGGC TAGATCAAAA CCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065843.4
    CDS84..1310
    Translation

    Target ORF information:

    RefSeq Version NM_020792.5
    Organism Homo sapiens(human)
    Definition Homo sapiens neutral cholesterol ester hydrolase 1 (NCEH1), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020792.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAGGTCGT CCTGTGTCCT GCTCACCGCC CTGGTGGCGC TGGCCGCCTA TTACGTCTAC 
    ATCCCGCTGC CTGGCTCCGT GTCCGACCCC TGGAAGCTGA TGCTGCTGGA CGCCACTTTC
    CGGGGTGCAC AGCAAGTGAG TAACCTGATC CACTACCTGG GACTGAGCCA TCACCTGCTG
    GCACTGAATT TTATCATTGT TTCTTTTGGC AAAAAAAGCG CGTGGTCTTC TGCCCAAGTG
    AAGGTGACCG ACACAGACTT TGATGGTGTG GAAGTCAGAG TGTTTGAAGG CCCTCCGAAG
    CCCGAAGAGC CACTGAAACG CAGCGTCGTT TATATCCACG GAGGAGGCTG GGCCTTGGCA
    AGTGCAAAAA TCAGGTATTA TGATGAGCTG TGTACAGCAA TGGCTGAGGA ATTGAATGCT
    GTCATTGTTT CCATTGAATA CAGGCTAGTT CCAAAGGTTT ATTTTCCTGA GCAAATTCAT
    GATGTTGTAC GGGCCACAAA GTATTTCCTG AAGCCAGAAG TCTTACAGAA GTATATGGTT
    GATCCAGGCA GAATTTGCAT TTCTGGTGAC AGTGCTGGTG GAAATCTGGC TGCTGCCCTT
    GGACAACAGT TTACTCAAGA TGCCAGCCTA AAAAATAAGC TCAAACTACA AGCTTTAATT
    TATCCAGTTC TTCAAGCTTT AGATTTTAAC ACACCATCTT ATCAGCAAAA TGTGAACACC
    CCAATCCTGC CCCGCTATGT CATGGTGAAG TATTGGGTGG ACTACTTCAA AGGCAACTAT
    GACTTTGTGC AGGCAATGAT CGTTAACAAT CACACTTCAC TTGATGTGGA AGAGGCTGCT
    GCTGTCAGGG CCCGTCTAAA CTGGACATCC CTCTTGCCTG CATCCTTCAC AAAGAACTAC
    AAGCCTGTTG TACAGACCAC AGGCAATGCC AGGATTGTCC AGGAGCTTCC TCAGTTGCTG
    GATGCCCGCT CCGCCCCACT CATTGCAGAC CAGGCAGTGC TGCAGCTCCT CCCAAAGACC
    TACATTCTGA CGTGTGAGCA TGATGTCCTC AGAGACGATG GCATCATGTA TGCCAAGCGT
    TTGGAGAGTG CCGGTGTGGA GGTGACCCTG GATCACTTTG AGGATGGCTT TCACGGATGT
    ATGATTTTCA CTAGCTGGCC CACCAACTTC TCAGTGGGAA TCCGGACTAG GAATAGTTAC
    ATCAAGTGGC TAGATCAAAA CCTGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.