RALGAPB cDNA ORF clone, Homo sapiens(human)

The following RALGAPB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the RALGAPB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu93082 XM_017027970.2
Latest version!
Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu93079 XM_017027967.2
Latest version!
Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu93080 XM_017027968.2
Latest version!
Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu93081 XM_017027969.2
Latest version!
Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu93078 XM_017027966.2
Latest version!
Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu00394 NM_020336.4
Latest version!
Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $384.30
$549.00
OHu00394 NM_001282917.2
Latest version!
Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $384.30
$549.00
OHu05184 NM_001282918.2
Latest version!
Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu53051 XM_005260462.3
Latest version!
Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu53052 XM_005260463.3
Latest version!
Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu53053 XM_005260464.3
Latest version!
Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu53054 XM_005260465.3
Latest version!
Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
View Old Accession Versions >>
OHu53052 XM_005260463.2 Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu00394 NM_001282917.1 Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $384.30
$549.00
OHu93081 XM_017027969.1 Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu93082 XM_017027970.1 Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OHu53051 XM_005260462.2 Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu93080 XM_017027968.1 Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu93078 XM_017027966.1 Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OHu53054 XM_005260465.2 Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu93079 XM_017027967.1 Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OHu00394 NM_020336.3 Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $384.30
$549.00
OHu53053 XM_005260464.2 Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu05184 NM_001282918.1 Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu93082
    Clone ID Related Accession (Same CDS sequence) XM_017027970.2 , XM_017027970.1
    Accession Version XM_017027970.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017027970.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGCCAC CATTTTGGAA AACAGCTTGG CTGTTCCTTA AAAAGGTAAA AGTGATGGTT 
    GACTCAGGAG ACCGGAAGCG AGCCATCAGT TCTGTGTGCA CCTACATTGT TTATCAGTGT
    AGTCGGCCAG CTCCTTTACA CTCCAGGGAT CTGCACTCCA TGATAGTGGC AGCTTTTCAG
    TGTCTCTGTG TCTGGCTGAC AGAGCACCCT GATATGCTTG ATGAAAAGGA CTGCCTTAAG
    GAAGTACTGG AGATTGTGGA ACTGGGTATC TCAGGAAGTA AGTCCAAGAA CAATGAGCAA
    GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA AACCCTGCAT CTATGAGGGT AAAGGATGCT
    GCTGAAGCCA CCCTAACATG CATTATGCAG TTGCTCGGCG CATTTCCTTC ACCTAGTGGT
    CCTGCCTCTC CTTGTAGTCT TGTGAATGAG ACCACTTTGA TTAAATACTC CAGGCTGCCA
    ACCATAAACA AGCATAGTTT CCGGTACTTT GTCTTGGATA ACAGTGTCAT CCTGGCAATG
    CTGGAACAAC CTCTTGGAAA TGAGCAGAAT GATTTTTTCC CCTCTGTCAC TGTGCTGGTC
    CGGGGAATGT CTGGAAGACT TGCTTGGGCA CAACAGCTTT GTCTTTTACC CAGAGGAGCA
    AAAGCAAATC AGAAGCTTTT TGTACCTGAA CCTCGCCCAG TTCCTAAAAA TGACGTTGGA
    TTTAAATATT CTGTGAAACA TCGGCCATTT CCTGAAGAGG TGGACAAGAT TCCTTTTGTG
    AAAGCAGATC TCAGCATTCC AGATTTGCAT GAAATAGTCA CTGAAGAATT AGAAGAGAGA
    CACGAAAAAT TAAGGAGTGG CATGGCCCAG CAGATTGCTT ATGAAATACA CCTTGAGCAA
    CAGAGTGAGG AGGAATTGCA GAAGAGAAGT TTTCCTGACC CAGTTACGGA TTGCAAGCCC
    CCGCCTCCTG CCCAGGAATT CCAAACAGCC CGCCTTTTTC TCTCACACTT TGGATTTTTG
    TCCTTAGAAG CACTGAAGGA ACCTGCAAAT AGTCGTCTAC CTCCTCACCT TATTGCACTT
    GATTCCACGA TACCTGGATT TTTTGATGAC ATTGGGTATC TGGATCTCTT GCCATGTCGT
    CCTTTTGACA CAGTTTTTAT TTTCTATATG AAGCCAGGTC AGAAAACGAA CCAAGAGATT
    TTAAAGAATG TGGAGTCTTC CAGAACTGTT CAGCCACATT TCCTAGAATT TTTGCTTTCC
    CTTGGCTGGT CAGTAGATGT GGGCAGACAC CCTGGTTGGA CTGGGCATGT TTCTACCAGT
    TGGTCTATTA ATTGTTGTGA TGATGGTGAA GGATCTCAAC AAGAAGAAGT GATTTCCTCT
    GAAGATATTG GAGCTAGCAT TTTCAATGGA CAGAAGAAGG TGCTGTATTA TGCTGATGCC
    CTTACAGAAA TTGCTTTTGT GGTTCCTTCT CCTGTGGAGT CCTTAACTGA TTCATTGGAA
    AGTAACATCT CGGACCAAGA TAGTGATTCA AATATGGATC TTATGCCAGG AATTCTGAAA
    CAGCCATCCC TGACACTTGA GCTTTTCCCC AATCATACAG ACAATCTTAA TTCCTCACAG
    AGGCTCAGTC CCAGTTCCAG AATGAGGAAG CTGCCTCAGG GTCGCCCTGT TCCTCCCCTT
    GGACCTGAGA CAAGAGTTTC TGTAGTCTGG GTGGAACGCT ATGATGATAT AGAAAACTTT
    CCCCTCTCAG AGCTGATGAC AGAGATCAGT ACTGGTGTGG AAACTACTGC AAATAGTAGC
    ACTTCACTGA GATCTACAAC TCTTGAAAAA GAAGTTCCTG TCATCTTCAT CCACCCTTTA
    AACACTGGAT TATTCCGGAT AAAAATTCAA GGAGCCACTG GAAAATTTAA TATGGTCATC
    CCTCTTGTGG ATGGGATGAT TGTCAGCAGG CGAGCTCTTG GCTTTCTGGT GAGGCAGACT
    GTAATTAACA TTTGTAGAAG AAAGAGACTG GAAAGTGACT CCTACAGTCC CCCCCATGTC
    CGCCGGAAAC AGAAAATCAC CGACATTGTC AACAAGTACC GGAACAAGCA GCTGGAGCCA
    GAGTTTTATA CTTCACTTTT CCAGGAGGTT GGACTCAAGA ACTGCAGTTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883459.1
    CDS111..2264
    Translation

    Target ORF information:

    RefSeq Version XM_017027970.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027970.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGCCAC CATTTTGGAA AACAGCTTGG CTGTTCCTTA AAAAGGTAAA AGTGATGGTT 
    GACTCAGGAG ACCGGAAGCG AGCCATCAGT TCTGTGTGCA CCTACATTGT TTATCAGTGT
    AGTCGGCCAG CTCCTTTACA CTCCAGGGAT CTGCACTCCA TGATAGTGGC AGCTTTTCAG
    TGTCTCTGTG TCTGGCTGAC AGAGCACCCT GATATGCTTG ATGAAAAGGA CTGCCTTAAG
    GAAGTACTGG AGATTGTGGA ACTGGGTATC TCAGGAAGTA AGTCCAAGAA CAATGAGCAA
    GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA AACCCTGCAT CTATGAGGGT AAAGGATGCT
    GCTGAAGCCA CCCTAACATG CATTATGCAG TTGCTCGGCG CATTTCCTTC ACCTAGTGGT
    CCTGCCTCTC CTTGTAGTCT TGTGAATGAG ACCACTTTGA TTAAATACTC CAGGCTGCCA
    ACCATAAACA AGCATAGTTT CCGGTACTTT GTCTTGGATA ACAGTGTCAT CCTGGCAATG
    CTGGAACAAC CTCTTGGAAA TGAGCAGAAT GATTTTTTCC CCTCTGTCAC TGTGCTGGTC
    CGGGGAATGT CTGGAAGACT TGCTTGGGCA CAACAGCTTT GTCTTTTACC CAGAGGAGCA
    AAAGCAAATC AGAAGCTTTT TGTACCTGAA CCTCGCCCAG TTCCTAAAAA TGACGTTGGA
    TTTAAATATT CTGTGAAACA TCGGCCATTT CCTGAAGAGG TGGACAAGAT TCCTTTTGTG
    AAAGCAGATC TCAGCATTCC AGATTTGCAT GAAATAGTCA CTGAAGAATT AGAAGAGAGA
    CACGAAAAAT TAAGGAGTGG CATGGCCCAG CAGATTGCTT ATGAAATACA CCTTGAGCAA
    CAGAGTGAGG AGGAATTGCA GAAGAGAAGT TTTCCTGACC CAGTTACGGA TTGCAAGCCC
    CCGCCTCCTG CCCAGGAATT CCAAACAGCC CGCCTTTTTC TCTCACACTT TGGATTTTTG
    TCCTTAGAAG CACTGAAGGA ACCTGCAAAT AGTCGTCTAC CTCCTCACCT TATTGCACTT
    GATTCCACGA TACCTGGATT TTTTGATGAC ATTGGGTATC TGGATCTCTT GCCATGTCGT
    CCTTTTGACA CAGTTTTTAT TTTCTATATG AAGCCAGGTC AGAAAACGAA CCAAGAGATT
    TTAAAGAATG TGGAGTCTTC CAGAACTGTT CAGCCACATT TCCTAGAATT TTTGCTTTCC
    CTTGGCTGGT CAGTAGATGT GGGCAGACAC CCTGGTTGGA CTGGGCATGT TTCTACCAGT
    TGGTCTATTA ATTGTTGTGA TGATGGTGAA GGATCTCAAC AAGAAGAAGT GATTTCCTCT
    GAAGATATTG GAGCTAGCAT TTTCAATGGA CAGAAGAAGG TGCTGTATTA TGCTGATGCC
    CTTACAGAAA TTGCTTTTGT GGTTCCTTCT CCTGTGGAGT CCTTAACTGA TTCATTGGAA
    AGTAACATCT CGGACCAAGA TAGTGATTCA AATATGGATC TTATGCCAGG AATTCTGAAA
    CAGCCATCCC TGACACTTGA GCTTTTCCCC AATCATACAG ACAATCTTAA TTCCTCACAG
    AGGCTCAGTC CCAGTTCCAG AATGAGGAAG CTGCCTCAGG GTCGCCCTGT TCCTCCCCTT
    GGACCTGAGA CAAGAGTTTC TGTAGTCTGG GTGGAACGCT ATGATGATAT AGAAAACTTT
    CCCCTCTCAG AGCTGATGAC AGAGATCAGT ACTGGTGTGG AAACTACTGC AAATAGTAGC
    ACTTCACTGA GATCTACAAC TCTTGAAAAA GAAGTTCCTG TCATCTTCAT CCACCCTTTA
    AACACTGGAT TATTCCGGAT AAAAATTCAA GGAGCCACTG GAAAATTTAA TATGGTCATC
    CCTCTTGTGG ATGGGATGAT TGTCAGCAGG CGAGCTCTTG GCTTTCTGGT GAGGCAGACT
    GTAATTAACA TTTGTAGAAG AAAGAGACTG GAAAGTGACT CCTACAGTCC CCCCCATGTC
    CGCCGGAAAC AGAAAATCAC CGACATTGTC AACAAGTACC GGAACAAGCA GCTGGAGCCA
    GAGTTTTATA CTTCACTTTT CCAGGAGGTT GGACTCAAGA ACTGCAGTTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93082
    Clone ID Related Accession (Same CDS sequence) XM_017027970.2 , XM_017027970.1
    Accession Version XM_017027970.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2154bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGCCAC CATTTTGGAA AACAGCTTGG CTGTTCCTTA AAAAGGTAAA AGTGATGGTT 
    GACTCAGGAG ACCGGAAGCG AGCCATCAGT TCTGTGTGCA CCTACATTGT TTATCAGTGT
    AGTCGGCCAG CTCCTTTACA CTCCAGGGAT CTGCACTCCA TGATAGTGGC AGCTTTTCAG
    TGTCTCTGTG TCTGGCTGAC AGAGCACCCT GATATGCTTG ATGAAAAGGA CTGCCTTAAG
    GAAGTACTGG AGATTGTGGA ACTGGGTATC TCAGGAAGTA AGTCCAAGAA CAATGAGCAA
    GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA AACCCTGCAT CTATGAGGGT AAAGGATGCT
    GCTGAAGCCA CCCTAACATG CATTATGCAG TTGCTCGGCG CATTTCCTTC ACCTAGTGGT
    CCTGCCTCTC CTTGTAGTCT TGTGAATGAG ACCACTTTGA TTAAATACTC CAGGCTGCCA
    ACCATAAACA AGCATAGTTT CCGGTACTTT GTCTTGGATA ACAGTGTCAT CCTGGCAATG
    CTGGAACAAC CTCTTGGAAA TGAGCAGAAT GATTTTTTCC CCTCTGTCAC TGTGCTGGTC
    CGGGGAATGT CTGGAAGACT TGCTTGGGCA CAACAGCTTT GTCTTTTACC CAGAGGAGCA
    AAAGCAAATC AGAAGCTTTT TGTACCTGAA CCTCGCCCAG TTCCTAAAAA TGACGTTGGA
    TTTAAATATT CTGTGAAACA TCGGCCATTT CCTGAAGAGG TGGACAAGAT TCCTTTTGTG
    AAAGCAGATC TCAGCATTCC AGATTTGCAT GAAATAGTCA CTGAAGAATT AGAAGAGAGA
    CACGAAAAAT TAAGGAGTGG CATGGCCCAG CAGATTGCTT ATGAAATACA CCTTGAGCAA
    CAGAGTGAGG AGGAATTGCA GAAGAGAAGT TTTCCTGACC CAGTTACGGA TTGCAAGCCC
    CCGCCTCCTG CCCAGGAATT CCAAACAGCC CGCCTTTTTC TCTCACACTT TGGATTTTTG
    TCCTTAGAAG CACTGAAGGA ACCTGCAAAT AGTCGTCTAC CTCCTCACCT TATTGCACTT
    GATTCCACGA TACCTGGATT TTTTGATGAC ATTGGGTATC TGGATCTCTT GCCATGTCGT
    CCTTTTGACA CAGTTTTTAT TTTCTATATG AAGCCAGGTC AGAAAACGAA CCAAGAGATT
    TTAAAGAATG TGGAGTCTTC CAGAACTGTT CAGCCACATT TCCTAGAATT TTTGCTTTCC
    CTTGGCTGGT CAGTAGATGT GGGCAGACAC CCTGGTTGGA CTGGGCATGT TTCTACCAGT
    TGGTCTATTA ATTGTTGTGA TGATGGTGAA GGATCTCAAC AAGAAGAAGT GATTTCCTCT
    GAAGATATTG GAGCTAGCAT TTTCAATGGA CAGAAGAAGG TGCTGTATTA TGCTGATGCC
    CTTACAGAAA TTGCTTTTGT GGTTCCTTCT CCTGTGGAGT CCTTAACTGA TTCATTGGAA
    AGTAACATCT CGGACCAAGA TAGTGATTCA AATATGGATC TTATGCCAGG AATTCTGAAA
    CAGCCATCCC TGACACTTGA GCTTTTCCCC AATCATACAG ACAATCTTAA TTCCTCACAG
    AGGCTCAGTC CCAGTTCCAG AATGAGGAAG CTGCCTCAGG GTCGCCCTGT TCCTCCCCTT
    GGACCTGAGA CAAGAGTTTC TGTAGTCTGG GTGGAACGCT ATGATGATAT AGAAAACTTT
    CCCCTCTCAG AGCTGATGAC AGAGATCAGT ACTGGTGTGG AAACTACTGC AAATAGTAGC
    ACTTCACTGA GATCTACAAC TCTTGAAAAA GAAGTTCCTG TCATCTTCAT CCACCCTTTA
    AACACTGGAT TATTCCGGAT AAAAATTCAA GGAGCCACTG GAAAATTTAA TATGGTCATC
    CCTCTTGTGG ATGGGATGAT TGTCAGCAGG CGAGCTCTTG GCTTTCTGGT GAGGCAGACT
    GTAATTAACA TTTGTAGAAG AAAGAGACTG GAAAGTGACT CCTACAGTCC CCCCCATGTC
    CGCCGGAAAC AGAAAATCAC CGACATTGTC AACAAGTACC GGAACAAGCA GCTGGAGCCA
    GAGTTTTATA CTTCACTTTT CCAGGAGGTT GGACTCAAGA ACTGCAGTTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883459.1
    CDS111..2264
    Translation

    Target ORF information:

    RefSeq Version XM_017027970.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027970.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGGAGCCAC CATTTTGGAA AACAGCTTGG CTGTTCCTTA AAAAGGTAAA AGTGATGGTT 
    GACTCAGGAG ACCGGAAGCG AGCCATCAGT TCTGTGTGCA CCTACATTGT TTATCAGTGT
    AGTCGGCCAG CTCCTTTACA CTCCAGGGAT CTGCACTCCA TGATAGTGGC AGCTTTTCAG
    TGTCTCTGTG TCTGGCTGAC AGAGCACCCT GATATGCTTG ATGAAAAGGA CTGCCTTAAG
    GAAGTACTGG AGATTGTGGA ACTGGGTATC TCAGGAAGTA AGTCCAAGAA CAATGAGCAA
    GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA AACCCTGCAT CTATGAGGGT AAAGGATGCT
    GCTGAAGCCA CCCTAACATG CATTATGCAG TTGCTCGGCG CATTTCCTTC ACCTAGTGGT
    CCTGCCTCTC CTTGTAGTCT TGTGAATGAG ACCACTTTGA TTAAATACTC CAGGCTGCCA
    ACCATAAACA AGCATAGTTT CCGGTACTTT GTCTTGGATA ACAGTGTCAT CCTGGCAATG
    CTGGAACAAC CTCTTGGAAA TGAGCAGAAT GATTTTTTCC CCTCTGTCAC TGTGCTGGTC
    CGGGGAATGT CTGGAAGACT TGCTTGGGCA CAACAGCTTT GTCTTTTACC CAGAGGAGCA
    AAAGCAAATC AGAAGCTTTT TGTACCTGAA CCTCGCCCAG TTCCTAAAAA TGACGTTGGA
    TTTAAATATT CTGTGAAACA TCGGCCATTT CCTGAAGAGG TGGACAAGAT TCCTTTTGTG
    AAAGCAGATC TCAGCATTCC AGATTTGCAT GAAATAGTCA CTGAAGAATT AGAAGAGAGA
    CACGAAAAAT TAAGGAGTGG CATGGCCCAG CAGATTGCTT ATGAAATACA CCTTGAGCAA
    CAGAGTGAGG AGGAATTGCA GAAGAGAAGT TTTCCTGACC CAGTTACGGA TTGCAAGCCC
    CCGCCTCCTG CCCAGGAATT CCAAACAGCC CGCCTTTTTC TCTCACACTT TGGATTTTTG
    TCCTTAGAAG CACTGAAGGA ACCTGCAAAT AGTCGTCTAC CTCCTCACCT TATTGCACTT
    GATTCCACGA TACCTGGATT TTTTGATGAC ATTGGGTATC TGGATCTCTT GCCATGTCGT
    CCTTTTGACA CAGTTTTTAT TTTCTATATG AAGCCAGGTC AGAAAACGAA CCAAGAGATT
    TTAAAGAATG TGGAGTCTTC CAGAACTGTT CAGCCACATT TCCTAGAATT TTTGCTTTCC
    CTTGGCTGGT CAGTAGATGT GGGCAGACAC CCTGGTTGGA CTGGGCATGT TTCTACCAGT
    TGGTCTATTA ATTGTTGTGA TGATGGTGAA GGATCTCAAC AAGAAGAAGT GATTTCCTCT
    GAAGATATTG GAGCTAGCAT TTTCAATGGA CAGAAGAAGG TGCTGTATTA TGCTGATGCC
    CTTACAGAAA TTGCTTTTGT GGTTCCTTCT CCTGTGGAGT CCTTAACTGA TTCATTGGAA
    AGTAACATCT CGGACCAAGA TAGTGATTCA AATATGGATC TTATGCCAGG AATTCTGAAA
    CAGCCATCCC TGACACTTGA GCTTTTCCCC AATCATACAG ACAATCTTAA TTCCTCACAG
    AGGCTCAGTC CCAGTTCCAG AATGAGGAAG CTGCCTCAGG GTCGCCCTGT TCCTCCCCTT
    GGACCTGAGA CAAGAGTTTC TGTAGTCTGG GTGGAACGCT ATGATGATAT AGAAAACTTT
    CCCCTCTCAG AGCTGATGAC AGAGATCAGT ACTGGTGTGG AAACTACTGC AAATAGTAGC
    ACTTCACTGA GATCTACAAC TCTTGAAAAA GAAGTTCCTG TCATCTTCAT CCACCCTTTA
    AACACTGGAT TATTCCGGAT AAAAATTCAA GGAGCCACTG GAAAATTTAA TATGGTCATC
    CCTCTTGTGG ATGGGATGAT TGTCAGCAGG CGAGCTCTTG GCTTTCTGGT GAGGCAGACT
    GTAATTAACA TTTGTAGAAG AAAGAGACTG GAAAGTGACT CCTACAGTCC CCCCCATGTC
    CGCCGGAAAC AGAAAATCAC CGACATTGTC AACAAGTACC GGAACAAGCA GCTGGAGCCA
    GAGTTTTATA CTTCACTTTT CCAGGAGGTT GGACTCAAGA ACTGCAGTTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93079
    Clone ID Related Accession (Same CDS sequence) XM_017027967.1 , XM_017027967.2
    Accession Version XM_017027967.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACCC AAAGACTCAA CTCCCAGTGG CGCCAAGACA TGAGCATATC ACTGGCAGCT
    CTAGAGCTCC TCTCTGGCCT TGCAAAGGTA AAAGTGATGG TTGACTCAGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CACCTACATT GTTTATCAGT GTAGTCGGCC AGCTCCTTTA
    CACTCCAGGG ATCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAGCACC CTGATATGCT TGATGAAAAG GACTGCCTTA AGGAAGTACT GGAGATTGTG
    GAACTGGGTA TCTCAGGAAG TAAGTCCAAG AACAATGAGC AAGAGGTCAA GTACAAAGGA
    GATAAGGAGC CAAACCCTGC ATCTATGAGG GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGCATTATGC AGTTGCTCGG CGCATTTCCT TCACCTAGTG GTCCTGCCTC TCCTTGTAGT
    CTTGTGAATG AGACCACTTT GATTAAATAC TCCAGGCTGC CAACCATAAA CAAGCATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAACA ACCTCTTGGA
    AATGAGCAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTGG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAGCT TTGTCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTACCTG AACCTCGCCC AGTTCCTAAA AATGACGTTG GATTTAAATA TTCTGTGAAA
    CATCGGCCAT TTCCTGAAGA GGTGGACAAG ATTCCTTTTG TGAAAGCAGA TCTCAGCATT
    CCAGATTTGC ATGAAATAGT CACTGAAGAA TTAGAAGAGA GACACGAAAA ATTAAGGAGT
    GGCATGGCCC AGCAGATTGC TTATGAAATA CACCTTGAGC AACAGAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA CCCAGTTACG GATTGCAAGC CCCCGCCTCC TGCCCAGGAA
    TTCCAAACAG CCCGCCTTTT TCTCTCACAC TTTGGATTTT TGTCCTTAGA AGCACTGAAG
    GAACCTGCAA ATAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGATTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCTTTTGA CACAGTTTTT
    ATTTTCTATA TGAAGCCAGG TCAGAAAACG AACCAAGAGA TTTTAAAGAA TGTGGAGTCT
    TCCAGAACTG TTCAGCCACA TTTCCTAGAA TTTTTGCTTT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAGAC ACCCTGGTTG GACTGGGCAT GTTTCTACCA GTTGGTCTAT TAATTGTTGT
    GATGATGGTG AAGGATCTCA ACAAGAAGTG ATTTCCTCTG AAGATATTGG AGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACAGAAAT TGCTTTTGTG
    GTTCCTTCTC CTGTGGAGTC CTTAACTGAT TCATTGGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCAGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCCA ATCATACAGA CAATCTTAAT TCCTCACAGA GGCTCAGTCC CAGTTCCAGA
    ATGAGGAAGC TGCCTCAGGG TCGCCCTGTT CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAAAACTTTC CCCTCTCAGA GCTGATGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AATAGTAGCA CTTCACTGAG ATCTACAACT
    CTTGAAAAAG AAGTTCCTGT CATCTTCATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAT ATGGTCATCC CTCTTGTGGA TGGGATGATT
    GTCAGCAGGC GAGCTCTTGG CTTTCTGGTG AGGCAGACTG TAATTAACAT TTGTAGAAGA
    AAGAGACTGG AAAGTGACTC CTACAGTCCC CCCCATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG CTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCAGTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883456.1
    CDS253..4725
    Translation

    Target ORF information:

    RefSeq Version XM_017027967.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027967.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACCC AAAGACTCAA CTCCCAGTGG CGCCAAGACA TGAGCATATC ACTGGCAGCT
    CTAGAGCTCC TCTCTGGCCT TGCAAAGGTA AAAGTGATGG TTGACTCAGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CACCTACATT GTTTATCAGT GTAGTCGGCC AGCTCCTTTA
    CACTCCAGGG ATCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAGCACC CTGATATGCT TGATGAAAAG GACTGCCTTA AGGAAGTACT GGAGATTGTG
    GAACTGGGTA TCTCAGGAAG TAAGTCCAAG AACAATGAGC AAGAGGTCAA GTACAAAGGA
    GATAAGGAGC CAAACCCTGC ATCTATGAGG GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGCATTATGC AGTTGCTCGG CGCATTTCCT TCACCTAGTG GTCCTGCCTC TCCTTGTAGT
    CTTGTGAATG AGACCACTTT GATTAAATAC TCCAGGCTGC CAACCATAAA CAAGCATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAACA ACCTCTTGGA
    AATGAGCAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTGG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAGCT TTGTCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTACCTG AACCTCGCCC AGTTCCTAAA AATGACGTTG GATTTAAATA TTCTGTGAAA
    CATCGGCCAT TTCCTGAAGA GGTGGACAAG ATTCCTTTTG TGAAAGCAGA TCTCAGCATT
    CCAGATTTGC ATGAAATAGT CACTGAAGAA TTAGAAGAGA GACACGAAAA ATTAAGGAGT
    GGCATGGCCC AGCAGATTGC TTATGAAATA CACCTTGAGC AACAGAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA CCCAGTTACG GATTGCAAGC CCCCGCCTCC TGCCCAGGAA
    TTCCAAACAG CCCGCCTTTT TCTCTCACAC TTTGGATTTT TGTCCTTAGA AGCACTGAAG
    GAACCTGCAA ATAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGATTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCTTTTGA CACAGTTTTT
    ATTTTCTATA TGAAGCCAGG TCAGAAAACG AACCAAGAGA TTTTAAAGAA TGTGGAGTCT
    TCCAGAACTG TTCAGCCACA TTTCCTAGAA TTTTTGCTTT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAGAC ACCCTGGTTG GACTGGGCAT GTTTCTACCA GTTGGTCTAT TAATTGTTGT
    GATGATGGTG AAGGATCTCA ACAAGAAGTG ATTTCCTCTG AAGATATTGG AGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACAGAAAT TGCTTTTGTG
    GTTCCTTCTC CTGTGGAGTC CTTAACTGAT TCATTGGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCAGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCCA ATCATACAGA CAATCTTAAT TCCTCACAGA GGCTCAGTCC CAGTTCCAGA
    ATGAGGAAGC TGCCTCAGGG TCGCCCTGTT CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAAAACTTTC CCCTCTCAGA GCTGATGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AATAGTAGCA CTTCACTGAG ATCTACAACT
    CTTGAAAAAG AAGTTCCTGT CATCTTCATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAT ATGGTCATCC CTCTTGTGGA TGGGATGATT
    GTCAGCAGGC GAGCTCTTGG CTTTCTGGTG AGGCAGACTG TAATTAACAT TTGTAGAAGA
    AAGAGACTGG AAAGTGACTC CTACAGTCCC CCCCATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG CTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCAGTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93079
    Clone ID Related Accession (Same CDS sequence) XM_017027967.1 , XM_017027967.2
    Accession Version XM_017027967.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017027967.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACCC AAAGACTCAA CTCCCAGTGG CGCCAAGACA TGAGCATATC ACTGGCAGCT
    CTAGAGCTCC TCTCTGGCCT TGCAAAGGTA AAAGTGATGG TTGACTCAGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CACCTACATT GTTTATCAGT GTAGTCGGCC AGCTCCTTTA
    CACTCCAGGG ATCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAGCACC CTGATATGCT TGATGAAAAG GACTGCCTTA AGGAAGTACT GGAGATTGTG
    GAACTGGGTA TCTCAGGAAG TAAGTCCAAG AACAATGAGC AAGAGGTCAA GTACAAAGGA
    GATAAGGAGC CAAACCCTGC ATCTATGAGG GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGCATTATGC AGTTGCTCGG CGCATTTCCT TCACCTAGTG GTCCTGCCTC TCCTTGTAGT
    CTTGTGAATG AGACCACTTT GATTAAATAC TCCAGGCTGC CAACCATAAA CAAGCATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAACA ACCTCTTGGA
    AATGAGCAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTGG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAGCT TTGTCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTACCTG AACCTCGCCC AGTTCCTAAA AATGACGTTG GATTTAAATA TTCTGTGAAA
    CATCGGCCAT TTCCTGAAGA GGTGGACAAG ATTCCTTTTG TGAAAGCAGA TCTCAGCATT
    CCAGATTTGC ATGAAATAGT CACTGAAGAA TTAGAAGAGA GACACGAAAA ATTAAGGAGT
    GGCATGGCCC AGCAGATTGC TTATGAAATA CACCTTGAGC AACAGAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA CCCAGTTACG GATTGCAAGC CCCCGCCTCC TGCCCAGGAA
    TTCCAAACAG CCCGCCTTTT TCTCTCACAC TTTGGATTTT TGTCCTTAGA AGCACTGAAG
    GAACCTGCAA ATAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGATTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCTTTTGA CACAGTTTTT
    ATTTTCTATA TGAAGCCAGG TCAGAAAACG AACCAAGAGA TTTTAAAGAA TGTGGAGTCT
    TCCAGAACTG TTCAGCCACA TTTCCTAGAA TTTTTGCTTT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAGAC ACCCTGGTTG GACTGGGCAT GTTTCTACCA GTTGGTCTAT TAATTGTTGT
    GATGATGGTG AAGGATCTCA ACAAGAAGTG ATTTCCTCTG AAGATATTGG AGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACAGAAAT TGCTTTTGTG
    GTTCCTTCTC CTGTGGAGTC CTTAACTGAT TCATTGGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCAGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCCA ATCATACAGA CAATCTTAAT TCCTCACAGA GGCTCAGTCC CAGTTCCAGA
    ATGAGGAAGC TGCCTCAGGG TCGCCCTGTT CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAAAACTTTC CCCTCTCAGA GCTGATGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AATAGTAGCA CTTCACTGAG ATCTACAACT
    CTTGAAAAAG AAGTTCCTGT CATCTTCATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAT ATGGTCATCC CTCTTGTGGA TGGGATGATT
    GTCAGCAGGC GAGCTCTTGG CTTTCTGGTG AGGCAGACTG TAATTAACAT TTGTAGAAGA
    AAGAGACTGG AAAGTGACTC CTACAGTCCC CCCCATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG CTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCAGTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883456.1
    CDS281..4753
    Translation

    Target ORF information:

    RefSeq Version XM_017027967.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027967.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACCC AAAGACTCAA CTCCCAGTGG CGCCAAGACA TGAGCATATC ACTGGCAGCT
    CTAGAGCTCC TCTCTGGCCT TGCAAAGGTA AAAGTGATGG TTGACTCAGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CACCTACATT GTTTATCAGT GTAGTCGGCC AGCTCCTTTA
    CACTCCAGGG ATCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAGCACC CTGATATGCT TGATGAAAAG GACTGCCTTA AGGAAGTACT GGAGATTGTG
    GAACTGGGTA TCTCAGGAAG TAAGTCCAAG AACAATGAGC AAGAGGTCAA GTACAAAGGA
    GATAAGGAGC CAAACCCTGC ATCTATGAGG GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGCATTATGC AGTTGCTCGG CGCATTTCCT TCACCTAGTG GTCCTGCCTC TCCTTGTAGT
    CTTGTGAATG AGACCACTTT GATTAAATAC TCCAGGCTGC CAACCATAAA CAAGCATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAACA ACCTCTTGGA
    AATGAGCAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTGG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAGCT TTGTCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTACCTG AACCTCGCCC AGTTCCTAAA AATGACGTTG GATTTAAATA TTCTGTGAAA
    CATCGGCCAT TTCCTGAAGA GGTGGACAAG ATTCCTTTTG TGAAAGCAGA TCTCAGCATT
    CCAGATTTGC ATGAAATAGT CACTGAAGAA TTAGAAGAGA GACACGAAAA ATTAAGGAGT
    GGCATGGCCC AGCAGATTGC TTATGAAATA CACCTTGAGC AACAGAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA CCCAGTTACG GATTGCAAGC CCCCGCCTCC TGCCCAGGAA
    TTCCAAACAG CCCGCCTTTT TCTCTCACAC TTTGGATTTT TGTCCTTAGA AGCACTGAAG
    GAACCTGCAA ATAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGATTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCTTTTGA CACAGTTTTT
    ATTTTCTATA TGAAGCCAGG TCAGAAAACG AACCAAGAGA TTTTAAAGAA TGTGGAGTCT
    TCCAGAACTG TTCAGCCACA TTTCCTAGAA TTTTTGCTTT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAGAC ACCCTGGTTG GACTGGGCAT GTTTCTACCA GTTGGTCTAT TAATTGTTGT
    GATGATGGTG AAGGATCTCA ACAAGAAGTG ATTTCCTCTG AAGATATTGG AGCTAGCATT
    TTCAATGGAC AGAAGAAGGT GCTGTATTAT GCTGATGCCC TTACAGAAAT TGCTTTTGTG
    GTTCCTTCTC CTGTGGAGTC CTTAACTGAT TCATTGGAAA GTAACATCTC GGACCAAGAT
    AGTGATTCAA ATATGGATCT TATGCCAGGA ATTCTGAAAC AGCCATCCCT GACACTTGAG
    CTTTTCCCCA ATCATACAGA CAATCTTAAT TCCTCACAGA GGCTCAGTCC CAGTTCCAGA
    ATGAGGAAGC TGCCTCAGGG TCGCCCTGTT CCTCCCCTTG GACCTGAGAC AAGAGTTTCT
    GTAGTCTGGG TGGAACGCTA TGATGATATA GAAAACTTTC CCCTCTCAGA GCTGATGACA
    GAGATCAGTA CTGGTGTGGA AACTACTGCA AATAGTAGCA CTTCACTGAG ATCTACAACT
    CTTGAAAAAG AAGTTCCTGT CATCTTCATC CACCCTTTAA ACACTGGATT ATTCCGGATA
    AAAATTCAAG GAGCCACTGG AAAATTTAAT ATGGTCATCC CTCTTGTGGA TGGGATGATT
    GTCAGCAGGC GAGCTCTTGG CTTTCTGGTG AGGCAGACTG TAATTAACAT TTGTAGAAGA
    AAGAGACTGG AAAGTGACTC CTACAGTCCC CCCCATGTCC GCCGGAAACA GAAAATCACC
    GACATTGTCA ACAAGTACCG GAACAAGCAG CTGGAGCCAG AGTTTTATAC TTCACTTTTC
    CAGGAGGTTG GACTCAAGAA CTGCAGTTCT TAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93080
    Clone ID Related Accession (Same CDS sequence) XM_017027968.2 , XM_017027968.1
    Accession Version XM_017027968.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017027968.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGGAGGA AGGAGCCTCA GTACCTGAGG AACAGACAGG AACAGGGTTC CAGTCAGATT 
    CGACTATGCT TACAGGTCCT GAGAGCCATT CAGAAACTGG CCCGTGAGTC ATCTCTCATG
    GCCCGAGAAA CTTGGGAAGT CTTACTGTTG TTTCTTCTGC AGATTAACGA CATACTTCTG
    GCCCCACCAA CTGTTCAAGG TGGCATTGCT GAGAATCTAG CAGAGAAGTT GATTGGTGTT
    CTCTTTGAGG TGTGGTTACT AGCTTGTACT CGGTGCTTCC CAACACCTCC TTATTGGAAA
    ACAGCCAAGG AGATGGTGGC TAACTGGAGG CATCACCCAG CAGTGGTGGA GCAGTGGAGC
    AAGGTCATTT GTGCACTCAC TTCCAGATTG CTACGCTTTA CATATGGTCC TTCATTTCCT
    GCATTTAAAG TTCCCGATGA AGATGCCAGT CTGATCCCTC CAGAAATGGA TAATGAGTGT
    GTTGCACAGA CATGGTTTCG CTTTTTACAC ATGTTAAGTA ATCCTGTGGA TTTGAGTAAC
    CCAGCTATTA TAAGCTCTAC TCCCAAATTT CAGGAACAGT TCTTGAATGT GAGCGGAATG
    CCGCAAGAAT TGAATCAGTA TCCCTGCCTT AAACATCTGC CTCAAATATT TTTTCGTGCC
    ATGCGTGGAA TCAGCTGTCT GGTGGATGCA TTCTTAGGTA TTTCTAGACC CCGATCAGAC
    AGTGCTCCCC CAACACCCGT GAATAGATTA AGTATGCCTC AAAGTGCTGC TGTCAGTACC
    ACCCCCCCAC ATAACCGGAG GCACCGGGCT GTTACTGTGA ATAAGGCCAC CATGAAGACA
    AGCACAGTTA GTACTGCTCA TGCCTCTAAA GTTCAGCACC AGACGTCCTC CACCTCTCCT
    CTGTCAAGTC CAAATCAGAC TAGTTCAGAA CCCCGGCCAC TGCCTGCCCC TCGGAGACCA
    AAGGTTAACA GCATCTTGAA TCTCTTTGGA TCATGGTTAT TTGATGCAGC ATTTGTTCAC
    TGTAAACTTC ATAATGGGAT AAACAGAGAC AGCAGCATGA CTGCATCTTT TATCCAAATT
    CTTCTTTCTT ATAAATCTTC CATTACAACA CAAGCTAGCA TGGAGTTTCG ACGGAAAGGG
    TCACAAATGT CCACAGACAC CATGGTTTCC AATCCTATGT TTGATGCAAG TGAATTTCCT
    GATAACTATG AAGCAGGAAG AGCTGAGGCT TGTGGGACAC TGTGTAGGAT TTTTTGTAGC
    AAGAAGACTG GAGAAGAGAT TCTGCCAGCT TATTTATCCA GATTTTACAT GCTTTTAATT
    CAAGGTTTGC AGATAAATGA TTATGTGTGC CATCCTGTCT TGGCCAGCGT TATTCTAAAC
    TCTCCTCCTT TGTTCTGCTG TGACTTGAAA GGGATTGATG TTGTGGTTCC TTACTTTATT
    TCAGCTCTTG AAACCATTTT GCCTGACAGA GAACTCTCAA AATTCAAAAG CTATGTAAAT
    CCAACAGAAT TGCGAAGATC CTCCATTAAT ATCCTGCTTT CTTTGTTGCC CCTCCCTCAT
    CATTTTGGCA CAGTCAAATC TGAGGTGGTC CTGGAAGGAA AGTTTAGTAA CGATGACAGC
    TCTTCTTATG ATAAACCAAT AACTTTTCTG TCCCTGAAGT TGAGACTTGT GAATATATTA
    ATAGGTGCCT TGCAAACTGA AACGGACCCC AACAACACCC AAATGATATT AGGGGCAATG
    TTAAATATTG TTCAAGATTC AGCACTTTTG GAAGCCATTG GTTGCCAGAT GGAGATGGGT
    GGTGGAGAAA ATAACCTGAA GAGTCATAGT CGCACCAATA GTGGTATTAG TTCAGCAAGT
    GGTGGAAGCA CGGAGCCCAC GACTCCCGAT AGTGAGAGAC CTGCTCAAGC TCTCTTAAGA
    GATTATGCTC TTAATACAGA TTCAGCTGCT GGGCTCCTGA TTCGCAGCAT TCATCTCGTC
    ACCCAAAGAC TCAACTCCCA GTGGCGCCAA GACATGAGCA TATCACTGGC AGCTCTAGAG
    CTCCTCTCTG GCCTTGCAAA GGTAAAAGTG ATGGTTGACT CAGGAGACCG GAAGCGAGCC
    ATCAGTTCTG TGTGCACCTA CATTGTTTAT CAGTGTAGTC GGCCAGCTCC TTTACACTCC
    AGGGATCTGC ACTCCATGAT AGTGGCAGCT TTTCAGTGTC TCTGTGTCTG GCTGACAGAG
    CACCCTGATA TGCTTGATGA AAAGGACTGC CTTAAGGAAG TACTGGAGAT TGTGGAACTG
    GGTATCTCAG GAAGTAAGTC CAAGAACAAT GAGCAAGAGG TCAAGTACAA AGGAGATAAG
    GAGCCAAACC CTGCATCTAT GAGGGTAAAG GATGCTGCTG AAGCCACCCT AACATGCATT
    ATGCAGTTGC TCGGCGCATT TCCTTCACCT AGTGGTCCTG CCTCTCCTTG TAGTCTTGTG
    AATGAGACCA CTTTGATTAA ATACTCCAGG CTGCCAACCA TAAACAAGCA TAGTTTCCGG
    TACTTTGTCT TGGATAACAG TGTCATCCTG GCAATGCTGG AACAACCTCT TGGAAATGAG
    CAGAATGATT TTTTCCCCTC TGTCACTGTG CTGGTCCGGG GAATGTCTGG AAGACTTGCT
    TGGGCACAAC AGCTTTGTCT TTTACCCAGA GGAGCAAAAG CAAATCAGAA GCTTTTTGTA
    CCTGAACCTC GCCCAGTTCC TAAAAATGAC GTTGGATTTA AATATTCTGT GAAACATCGG
    CCATTTCCTG AAGAGGTGGA CAAGATTCCT TTTGTGAAAG CAGATCTCAG CATTCCAGAT
    TTGCATGAAA TAGTCACTGA AGAATTAGAA GAGAGACACG AAAAATTAAG GAGTGGCATG
    GCCCAGCAGA TTGCTTATGA AATACACCTT GAGCAACAGA GTGAGGAGGA ATTGCAGAAG
    AGAAGTTTTC CTGACCCAGT TACGGATTGC AAGCCCCCGC CTCCTGCCCA GGAATTCCAA
    ACAGCCCGCC TTTTTCTCTC ACACTTTGGA TTTTTGTCCT TAGAAGCACT GAAGGAACCT
    GCAAATAGTC GTCTACCTCC TCACCTTATT GCACTTGATT CCACGATACC TGGATTTTTT
    GATGACATTG GGTATCTGGA TCTCTTGCCA TGTCGTCCTT TTGACACAGT TTTTATTTTC
    TATATGAAGC CAGGTCAGAA AACGAACCAA GAGATTTTAA AGAATGTGGA GTCTTCCAGA
    ACTGTTCAGC CACATTTCCT AGAATTTTTG CTTTCCCTTG GCTGGTCAGT AGATGTGGGC
    AGACACCCTG GTTGGACTGG GCATGTTTCT ACCAGTTGGT CTATTAATTG TTGTGATGAT
    GGTGAAGGAT CTCAACAAGA AGAAGTGATT TCCTCTGAAG ATATTGGAGC TAGCATTTTC
    AATGGACAGA AGAAGGTGCT GTATTATGCT GATGCCCTTA CAGAAATTGC TTTTGTGGTT
    CCTTCTCCTG TGGAGTCCTT AACTGATTCA TTGGAAAGTA ACATCTCGGA CCAAGATAGT
    GATTCAAATA TGGATCTTAT GCCAGGAATT CTGAAACAGC CATCCCTGAC ACTTGAGCTT
    TTCCCCAATC ATACAGACAA TCTTAATTCC TCACAGAGGC TCAGTCCCAG TTCCAGAATG
    AGGAAGCTGC CTCAGGGTCG CCCTGTTCCT CCCCTTGGAC CTGAGACAAG AGTTTCTGTA
    GTCTGGGTGG AACGCTATGA TGATATAGAA AACTTTCCCC TCTCAGAGCT GATGACAGAG
    ATCAGTACTG GTGTGGAAAC TACTGCAAAT AGTAGCACTT CACTGAGATC TACAACTCTT
    GAAAAAGAAG TTCCTGTCAT CTTCATCCAC CCTTTAAACA CTGGATTATT CCGGATAAAA
    ATTCAAGGAG CCACTGGAAA ATTTAATATG GTCATCCCTC TTGTGGATGG GATGATTGTC
    AGCAGGCGAG CTCTTGGCTT TCTGGTGAGG CAGACTGTAA TTAACATTTG TAGAAGAAAG
    AGACTGGAAA GTGACTCCTA CAGTCCCCCC CATGTCCGCC GGAAACAGAA AATCACCGAC
    ATTGTCAACA AGTACCGGAA CAAGCAGCTG GAGCCAGAGT TTTATACTTC ACTTTTCCAG
    GAGGTTGGAC TCAAGAACTG CAGTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883457.1
    CDS133..4302
    Translation

    Target ORF information:

    RefSeq Version XM_017027968.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027968.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGGAGGA AGGAGCCTCA GTACCTGAGG AACAGACAGG AACAGGGTTC CAGTCAGATT 
    CGACTATGCT TACAGGTCCT GAGAGCCATT CAGAAACTGG CCCGTGAGTC ATCTCTCATG
    GCCCGAGAAA CTTGGGAAGT CTTACTGTTG TTTCTTCTGC AGATTAACGA CATACTTCTG
    GCCCCACCAA CTGTTCAAGG TGGCATTGCT GAGAATCTAG CAGAGAAGTT GATTGGTGTT
    CTCTTTGAGG TGTGGTTACT AGCTTGTACT CGGTGCTTCC CAACACCTCC TTATTGGAAA
    ACAGCCAAGG AGATGGTGGC TAACTGGAGG CATCACCCAG CAGTGGTGGA GCAGTGGAGC
    AAGGTCATTT GTGCACTCAC TTCCAGATTG CTACGCTTTA CATATGGTCC TTCATTTCCT
    GCATTTAAAG TTCCCGATGA AGATGCCAGT CTGATCCCTC CAGAAATGGA TAATGAGTGT
    GTTGCACAGA CATGGTTTCG CTTTTTACAC ATGTTAAGTA ATCCTGTGGA TTTGAGTAAC
    CCAGCTATTA TAAGCTCTAC TCCCAAATTT CAGGAACAGT TCTTGAATGT GAGCGGAATG
    CCGCAAGAAT TGAATCAGTA TCCCTGCCTT AAACATCTGC CTCAAATATT TTTTCGTGCC
    ATGCGTGGAA TCAGCTGTCT GGTGGATGCA TTCTTAGGTA TTTCTAGACC CCGATCAGAC
    AGTGCTCCCC CAACACCCGT GAATAGATTA AGTATGCCTC AAAGTGCTGC TGTCAGTACC
    ACCCCCCCAC ATAACCGGAG GCACCGGGCT GTTACTGTGA ATAAGGCCAC CATGAAGACA
    AGCACAGTTA GTACTGCTCA TGCCTCTAAA GTTCAGCACC AGACGTCCTC CACCTCTCCT
    CTGTCAAGTC CAAATCAGAC TAGTTCAGAA CCCCGGCCAC TGCCTGCCCC TCGGAGACCA
    AAGGTTAACA GCATCTTGAA TCTCTTTGGA TCATGGTTAT TTGATGCAGC ATTTGTTCAC
    TGTAAACTTC ATAATGGGAT AAACAGAGAC AGCAGCATGA CTGCATCTTT TATCCAAATT
    CTTCTTTCTT ATAAATCTTC CATTACAACA CAAGCTAGCA TGGAGTTTCG ACGGAAAGGG
    TCACAAATGT CCACAGACAC CATGGTTTCC AATCCTATGT TTGATGCAAG TGAATTTCCT
    GATAACTATG AAGCAGGAAG AGCTGAGGCT TGTGGGACAC TGTGTAGGAT TTTTTGTAGC
    AAGAAGACTG GAGAAGAGAT TCTGCCAGCT TATTTATCCA GATTTTACAT GCTTTTAATT
    CAAGGTTTGC AGATAAATGA TTATGTGTGC CATCCTGTCT TGGCCAGCGT TATTCTAAAC
    TCTCCTCCTT TGTTCTGCTG TGACTTGAAA GGGATTGATG TTGTGGTTCC TTACTTTATT
    TCAGCTCTTG AAACCATTTT GCCTGACAGA GAACTCTCAA AATTCAAAAG CTATGTAAAT
    CCAACAGAAT TGCGAAGATC CTCCATTAAT ATCCTGCTTT CTTTGTTGCC CCTCCCTCAT
    CATTTTGGCA CAGTCAAATC TGAGGTGGTC CTGGAAGGAA AGTTTAGTAA CGATGACAGC
    TCTTCTTATG ATAAACCAAT AACTTTTCTG TCCCTGAAGT TGAGACTTGT GAATATATTA
    ATAGGTGCCT TGCAAACTGA AACGGACCCC AACAACACCC AAATGATATT AGGGGCAATG
    TTAAATATTG TTCAAGATTC AGCACTTTTG GAAGCCATTG GTTGCCAGAT GGAGATGGGT
    GGTGGAGAAA ATAACCTGAA GAGTCATAGT CGCACCAATA GTGGTATTAG TTCAGCAAGT
    GGTGGAAGCA CGGAGCCCAC GACTCCCGAT AGTGAGAGAC CTGCTCAAGC TCTCTTAAGA
    GATTATGCTC TTAATACAGA TTCAGCTGCT GGGCTCCTGA TTCGCAGCAT TCATCTCGTC
    ACCCAAAGAC TCAACTCCCA GTGGCGCCAA GACATGAGCA TATCACTGGC AGCTCTAGAG
    CTCCTCTCTG GCCTTGCAAA GGTAAAAGTG ATGGTTGACT CAGGAGACCG GAAGCGAGCC
    ATCAGTTCTG TGTGCACCTA CATTGTTTAT CAGTGTAGTC GGCCAGCTCC TTTACACTCC
    AGGGATCTGC ACTCCATGAT AGTGGCAGCT TTTCAGTGTC TCTGTGTCTG GCTGACAGAG
    CACCCTGATA TGCTTGATGA AAAGGACTGC CTTAAGGAAG TACTGGAGAT TGTGGAACTG
    GGTATCTCAG GAAGTAAGTC CAAGAACAAT GAGCAAGAGG TCAAGTACAA AGGAGATAAG
    GAGCCAAACC CTGCATCTAT GAGGGTAAAG GATGCTGCTG AAGCCACCCT AACATGCATT
    ATGCAGTTGC TCGGCGCATT TCCTTCACCT AGTGGTCCTG CCTCTCCTTG TAGTCTTGTG
    AATGAGACCA CTTTGATTAA ATACTCCAGG CTGCCAACCA TAAACAAGCA TAGTTTCCGG
    TACTTTGTCT TGGATAACAG TGTCATCCTG GCAATGCTGG AACAACCTCT TGGAAATGAG
    CAGAATGATT TTTTCCCCTC TGTCACTGTG CTGGTCCGGG GAATGTCTGG AAGACTTGCT
    TGGGCACAAC AGCTTTGTCT TTTACCCAGA GGAGCAAAAG CAAATCAGAA GCTTTTTGTA
    CCTGAACCTC GCCCAGTTCC TAAAAATGAC GTTGGATTTA AATATTCTGT GAAACATCGG
    CCATTTCCTG AAGAGGTGGA CAAGATTCCT TTTGTGAAAG CAGATCTCAG CATTCCAGAT
    TTGCATGAAA TAGTCACTGA AGAATTAGAA GAGAGACACG AAAAATTAAG GAGTGGCATG
    GCCCAGCAGA TTGCTTATGA AATACACCTT GAGCAACAGA GTGAGGAGGA ATTGCAGAAG
    AGAAGTTTTC CTGACCCAGT TACGGATTGC AAGCCCCCGC CTCCTGCCCA GGAATTCCAA
    ACAGCCCGCC TTTTTCTCTC ACACTTTGGA TTTTTGTCCT TAGAAGCACT GAAGGAACCT
    GCAAATAGTC GTCTACCTCC TCACCTTATT GCACTTGATT CCACGATACC TGGATTTTTT
    GATGACATTG GGTATCTGGA TCTCTTGCCA TGTCGTCCTT TTGACACAGT TTTTATTTTC
    TATATGAAGC CAGGTCAGAA AACGAACCAA GAGATTTTAA AGAATGTGGA GTCTTCCAGA
    ACTGTTCAGC CACATTTCCT AGAATTTTTG CTTTCCCTTG GCTGGTCAGT AGATGTGGGC
    AGACACCCTG GTTGGACTGG GCATGTTTCT ACCAGTTGGT CTATTAATTG TTGTGATGAT
    GGTGAAGGAT CTCAACAAGA AGAAGTGATT TCCTCTGAAG ATATTGGAGC TAGCATTTTC
    AATGGACAGA AGAAGGTGCT GTATTATGCT GATGCCCTTA CAGAAATTGC TTTTGTGGTT
    CCTTCTCCTG TGGAGTCCTT AACTGATTCA TTGGAAAGTA ACATCTCGGA CCAAGATAGT
    GATTCAAATA TGGATCTTAT GCCAGGAATT CTGAAACAGC CATCCCTGAC ACTTGAGCTT
    TTCCCCAATC ATACAGACAA TCTTAATTCC TCACAGAGGC TCAGTCCCAG TTCCAGAATG
    AGGAAGCTGC CTCAGGGTCG CCCTGTTCCT CCCCTTGGAC CTGAGACAAG AGTTTCTGTA
    GTCTGGGTGG AACGCTATGA TGATATAGAA AACTTTCCCC TCTCAGAGCT GATGACAGAG
    ATCAGTACTG GTGTGGAAAC TACTGCAAAT AGTAGCACTT CACTGAGATC TACAACTCTT
    GAAAAAGAAG TTCCTGTCAT CTTCATCCAC CCTTTAAACA CTGGATTATT CCGGATAAAA
    ATTCAAGGAG CCACTGGAAA ATTTAATATG GTCATCCCTC TTGTGGATGG GATGATTGTC
    AGCAGGCGAG CTCTTGGCTT TCTGGTGAGG CAGACTGTAA TTAACATTTG TAGAAGAAAG
    AGACTGGAAA GTGACTCCTA CAGTCCCCCC CATGTCCGCC GGAAACAGAA AATCACCGAC
    ATTGTCAACA AGTACCGGAA CAAGCAGCTG GAGCCAGAGT TTTATACTTC ACTTTTCCAG
    GAGGTTGGAC TCAAGAACTG CAGTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93080
    Clone ID Related Accession (Same CDS sequence) XM_017027968.2 , XM_017027968.1
    Accession Version XM_017027968.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4170bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGGAGGA AGGAGCCTCA GTACCTGAGG AACAGACAGG AACAGGGTTC CAGTCAGATT 
    CGACTATGCT TACAGGTCCT GAGAGCCATT CAGAAACTGG CCCGTGAGTC ATCTCTCATG
    GCCCGAGAAA CTTGGGAAGT CTTACTGTTG TTTCTTCTGC AGATTAACGA CATACTTCTG
    GCCCCACCAA CTGTTCAAGG TGGCATTGCT GAGAATCTAG CAGAGAAGTT GATTGGTGTT
    CTCTTTGAGG TGTGGTTACT AGCTTGTACT CGGTGCTTCC CAACACCTCC TTATTGGAAA
    ACAGCCAAGG AGATGGTGGC TAACTGGAGG CATCACCCAG CAGTGGTGGA GCAGTGGAGC
    AAGGTCATTT GTGCACTCAC TTCCAGATTG CTACGCTTTA CATATGGTCC TTCATTTCCT
    GCATTTAAAG TTCCCGATGA AGATGCCAGT CTGATCCCTC CAGAAATGGA TAATGAGTGT
    GTTGCACAGA CATGGTTTCG CTTTTTACAC ATGTTAAGTA ATCCTGTGGA TTTGAGTAAC
    CCAGCTATTA TAAGCTCTAC TCCCAAATTT CAGGAACAGT TCTTGAATGT GAGCGGAATG
    CCGCAAGAAT TGAATCAGTA TCCCTGCCTT AAACATCTGC CTCAAATATT TTTTCGTGCC
    ATGCGTGGAA TCAGCTGTCT GGTGGATGCA TTCTTAGGTA TTTCTAGACC CCGATCAGAC
    AGTGCTCCCC CAACACCCGT GAATAGATTA AGTATGCCTC AAAGTGCTGC TGTCAGTACC
    ACCCCCCCAC ATAACCGGAG GCACCGGGCT GTTACTGTGA ATAAGGCCAC CATGAAGACA
    AGCACAGTTA GTACTGCTCA TGCCTCTAAA GTTCAGCACC AGACGTCCTC CACCTCTCCT
    CTGTCAAGTC CAAATCAGAC TAGTTCAGAA CCCCGGCCAC TGCCTGCCCC TCGGAGACCA
    AAGGTTAACA GCATCTTGAA TCTCTTTGGA TCATGGTTAT TTGATGCAGC ATTTGTTCAC
    TGTAAACTTC ATAATGGGAT AAACAGAGAC AGCAGCATGA CTGCATCTTT TATCCAAATT
    CTTCTTTCTT ATAAATCTTC CATTACAACA CAAGCTAGCA TGGAGTTTCG ACGGAAAGGG
    TCACAAATGT CCACAGACAC CATGGTTTCC AATCCTATGT TTGATGCAAG TGAATTTCCT
    GATAACTATG AAGCAGGAAG AGCTGAGGCT TGTGGGACAC TGTGTAGGAT TTTTTGTAGC
    AAGAAGACTG GAGAAGAGAT TCTGCCAGCT TATTTATCCA GATTTTACAT GCTTTTAATT
    CAAGGTTTGC AGATAAATGA TTATGTGTGC CATCCTGTCT TGGCCAGCGT TATTCTAAAC
    TCTCCTCCTT TGTTCTGCTG TGACTTGAAA GGGATTGATG TTGTGGTTCC TTACTTTATT
    TCAGCTCTTG AAACCATTTT GCCTGACAGA GAACTCTCAA AATTCAAAAG CTATGTAAAT
    CCAACAGAAT TGCGAAGATC CTCCATTAAT ATCCTGCTTT CTTTGTTGCC CCTCCCTCAT
    CATTTTGGCA CAGTCAAATC TGAGGTGGTC CTGGAAGGAA AGTTTAGTAA CGATGACAGC
    TCTTCTTATG ATAAACCAAT AACTTTTCTG TCCCTGAAGT TGAGACTTGT GAATATATTA
    ATAGGTGCCT TGCAAACTGA AACGGACCCC AACAACACCC AAATGATATT AGGGGCAATG
    TTAAATATTG TTCAAGATTC AGCACTTTTG GAAGCCATTG GTTGCCAGAT GGAGATGGGT
    GGTGGAGAAA ATAACCTGAA GAGTCATAGT CGCACCAATA GTGGTATTAG TTCAGCAAGT
    GGTGGAAGCA CGGAGCCCAC GACTCCCGAT AGTGAGAGAC CTGCTCAAGC TCTCTTAAGA
    GATTATGCTC TTAATACAGA TTCAGCTGCT GGGCTCCTGA TTCGCAGCAT TCATCTCGTC
    ACCCAAAGAC TCAACTCCCA GTGGCGCCAA GACATGAGCA TATCACTGGC AGCTCTAGAG
    CTCCTCTCTG GCCTTGCAAA GGTAAAAGTG ATGGTTGACT CAGGAGACCG GAAGCGAGCC
    ATCAGTTCTG TGTGCACCTA CATTGTTTAT CAGTGTAGTC GGCCAGCTCC TTTACACTCC
    AGGGATCTGC ACTCCATGAT AGTGGCAGCT TTTCAGTGTC TCTGTGTCTG GCTGACAGAG
    CACCCTGATA TGCTTGATGA AAAGGACTGC CTTAAGGAAG TACTGGAGAT TGTGGAACTG
    GGTATCTCAG GAAGTAAGTC CAAGAACAAT GAGCAAGAGG TCAAGTACAA AGGAGATAAG
    GAGCCAAACC CTGCATCTAT GAGGGTAAAG GATGCTGCTG AAGCCACCCT AACATGCATT
    ATGCAGTTGC TCGGCGCATT TCCTTCACCT AGTGGTCCTG CCTCTCCTTG TAGTCTTGTG
    AATGAGACCA CTTTGATTAA ATACTCCAGG CTGCCAACCA TAAACAAGCA TAGTTTCCGG
    TACTTTGTCT TGGATAACAG TGTCATCCTG GCAATGCTGG AACAACCTCT TGGAAATGAG
    CAGAATGATT TTTTCCCCTC TGTCACTGTG CTGGTCCGGG GAATGTCTGG AAGACTTGCT
    TGGGCACAAC AGCTTTGTCT TTTACCCAGA GGAGCAAAAG CAAATCAGAA GCTTTTTGTA
    CCTGAACCTC GCCCAGTTCC TAAAAATGAC GTTGGATTTA AATATTCTGT GAAACATCGG
    CCATTTCCTG AAGAGGTGGA CAAGATTCCT TTTGTGAAAG CAGATCTCAG CATTCCAGAT
    TTGCATGAAA TAGTCACTGA AGAATTAGAA GAGAGACACG AAAAATTAAG GAGTGGCATG
    GCCCAGCAGA TTGCTTATGA AATACACCTT GAGCAACAGA GTGAGGAGGA ATTGCAGAAG
    AGAAGTTTTC CTGACCCAGT TACGGATTGC AAGCCCCCGC CTCCTGCCCA GGAATTCCAA
    ACAGCCCGCC TTTTTCTCTC ACACTTTGGA TTTTTGTCCT TAGAAGCACT GAAGGAACCT
    GCAAATAGTC GTCTACCTCC TCACCTTATT GCACTTGATT CCACGATACC TGGATTTTTT
    GATGACATTG GGTATCTGGA TCTCTTGCCA TGTCGTCCTT TTGACACAGT TTTTATTTTC
    TATATGAAGC CAGGTCAGAA AACGAACCAA GAGATTTTAA AGAATGTGGA GTCTTCCAGA
    ACTGTTCAGC CACATTTCCT AGAATTTTTG CTTTCCCTTG GCTGGTCAGT AGATGTGGGC
    AGACACCCTG GTTGGACTGG GCATGTTTCT ACCAGTTGGT CTATTAATTG TTGTGATGAT
    GGTGAAGGAT CTCAACAAGA AGAAGTGATT TCCTCTGAAG ATATTGGAGC TAGCATTTTC
    AATGGACAGA AGAAGGTGCT GTATTATGCT GATGCCCTTA CAGAAATTGC TTTTGTGGTT
    CCTTCTCCTG TGGAGTCCTT AACTGATTCA TTGGAAAGTA ACATCTCGGA CCAAGATAGT
    GATTCAAATA TGGATCTTAT GCCAGGAATT CTGAAACAGC CATCCCTGAC ACTTGAGCTT
    TTCCCCAATC ATACAGACAA TCTTAATTCC TCACAGAGGC TCAGTCCCAG TTCCAGAATG
    AGGAAGCTGC CTCAGGGTCG CCCTGTTCCT CCCCTTGGAC CTGAGACAAG AGTTTCTGTA
    GTCTGGGTGG AACGCTATGA TGATATAGAA AACTTTCCCC TCTCAGAGCT GATGACAGAG
    ATCAGTACTG GTGTGGAAAC TACTGCAAAT AGTAGCACTT CACTGAGATC TACAACTCTT
    GAAAAAGAAG TTCCTGTCAT CTTCATCCAC CCTTTAAACA CTGGATTATT CCGGATAAAA
    ATTCAAGGAG CCACTGGAAA ATTTAATATG GTCATCCCTC TTGTGGATGG GATGATTGTC
    AGCAGGCGAG CTCTTGGCTT TCTGGTGAGG CAGACTGTAA TTAACATTTG TAGAAGAAAG
    AGACTGGAAA GTGACTCCTA CAGTCCCCCC CATGTCCGCC GGAAACAGAA AATCACCGAC
    ATTGTCAACA AGTACCGGAA CAAGCAGCTG GAGCCAGAGT TTTATACTTC ACTTTTCCAG
    GAGGTTGGAC TCAAGAACTG CAGTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883457.1
    CDS133..4302
    Translation

    Target ORF information:

    RefSeq Version XM_017027968.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027968.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    ATGAGGAGGA AGGAGCCTCA GTACCTGAGG AACAGACAGG AACAGGGTTC CAGTCAGATT 
    CGACTATGCT TACAGGTCCT GAGAGCCATT CAGAAACTGG CCCGTGAGTC ATCTCTCATG
    GCCCGAGAAA CTTGGGAAGT CTTACTGTTG TTTCTTCTGC AGATTAACGA CATACTTCTG
    GCCCCACCAA CTGTTCAAGG TGGCATTGCT GAGAATCTAG CAGAGAAGTT GATTGGTGTT
    CTCTTTGAGG TGTGGTTACT AGCTTGTACT CGGTGCTTCC CAACACCTCC TTATTGGAAA
    ACAGCCAAGG AGATGGTGGC TAACTGGAGG CATCACCCAG CAGTGGTGGA GCAGTGGAGC
    AAGGTCATTT GTGCACTCAC TTCCAGATTG CTACGCTTTA CATATGGTCC TTCATTTCCT
    GCATTTAAAG TTCCCGATGA AGATGCCAGT CTGATCCCTC CAGAAATGGA TAATGAGTGT
    GTTGCACAGA CATGGTTTCG CTTTTTACAC ATGTTAAGTA ATCCTGTGGA TTTGAGTAAC
    CCAGCTATTA TAAGCTCTAC TCCCAAATTT CAGGAACAGT TCTTGAATGT GAGCGGAATG
    CCGCAAGAAT TGAATCAGTA TCCCTGCCTT AAACATCTGC CTCAAATATT TTTTCGTGCC
    ATGCGTGGAA TCAGCTGTCT GGTGGATGCA TTCTTAGGTA TTTCTAGACC CCGATCAGAC
    AGTGCTCCCC CAACACCCGT GAATAGATTA AGTATGCCTC AAAGTGCTGC TGTCAGTACC
    ACCCCCCCAC ATAACCGGAG GCACCGGGCT GTTACTGTGA ATAAGGCCAC CATGAAGACA
    AGCACAGTTA GTACTGCTCA TGCCTCTAAA GTTCAGCACC AGACGTCCTC CACCTCTCCT
    CTGTCAAGTC CAAATCAGAC TAGTTCAGAA CCCCGGCCAC TGCCTGCCCC TCGGAGACCA
    AAGGTTAACA GCATCTTGAA TCTCTTTGGA TCATGGTTAT TTGATGCAGC ATTTGTTCAC
    TGTAAACTTC ATAATGGGAT AAACAGAGAC AGCAGCATGA CTGCATCTTT TATCCAAATT
    CTTCTTTCTT ATAAATCTTC CATTACAACA CAAGCTAGCA TGGAGTTTCG ACGGAAAGGG
    TCACAAATGT CCACAGACAC CATGGTTTCC AATCCTATGT TTGATGCAAG TGAATTTCCT
    GATAACTATG AAGCAGGAAG AGCTGAGGCT TGTGGGACAC TGTGTAGGAT TTTTTGTAGC
    AAGAAGACTG GAGAAGAGAT TCTGCCAGCT TATTTATCCA GATTTTACAT GCTTTTAATT
    CAAGGTTTGC AGATAAATGA TTATGTGTGC CATCCTGTCT TGGCCAGCGT TATTCTAAAC
    TCTCCTCCTT TGTTCTGCTG TGACTTGAAA GGGATTGATG TTGTGGTTCC TTACTTTATT
    TCAGCTCTTG AAACCATTTT GCCTGACAGA GAACTCTCAA AATTCAAAAG CTATGTAAAT
    CCAACAGAAT TGCGAAGATC CTCCATTAAT ATCCTGCTTT CTTTGTTGCC CCTCCCTCAT
    CATTTTGGCA CAGTCAAATC TGAGGTGGTC CTGGAAGGAA AGTTTAGTAA CGATGACAGC
    TCTTCTTATG ATAAACCAAT AACTTTTCTG TCCCTGAAGT TGAGACTTGT GAATATATTA
    ATAGGTGCCT TGCAAACTGA AACGGACCCC AACAACACCC AAATGATATT AGGGGCAATG
    TTAAATATTG TTCAAGATTC AGCACTTTTG GAAGCCATTG GTTGCCAGAT GGAGATGGGT
    GGTGGAGAAA ATAACCTGAA GAGTCATAGT CGCACCAATA GTGGTATTAG TTCAGCAAGT
    GGTGGAAGCA CGGAGCCCAC GACTCCCGAT AGTGAGAGAC CTGCTCAAGC TCTCTTAAGA
    GATTATGCTC TTAATACAGA TTCAGCTGCT GGGCTCCTGA TTCGCAGCAT TCATCTCGTC
    ACCCAAAGAC TCAACTCCCA GTGGCGCCAA GACATGAGCA TATCACTGGC AGCTCTAGAG
    CTCCTCTCTG GCCTTGCAAA GGTAAAAGTG ATGGTTGACT CAGGAGACCG GAAGCGAGCC
    ATCAGTTCTG TGTGCACCTA CATTGTTTAT CAGTGTAGTC GGCCAGCTCC TTTACACTCC
    AGGGATCTGC ACTCCATGAT AGTGGCAGCT TTTCAGTGTC TCTGTGTCTG GCTGACAGAG
    CACCCTGATA TGCTTGATGA AAAGGACTGC CTTAAGGAAG TACTGGAGAT TGTGGAACTG
    GGTATCTCAG GAAGTAAGTC CAAGAACAAT GAGCAAGAGG TCAAGTACAA AGGAGATAAG
    GAGCCAAACC CTGCATCTAT GAGGGTAAAG GATGCTGCTG AAGCCACCCT AACATGCATT
    ATGCAGTTGC TCGGCGCATT TCCTTCACCT AGTGGTCCTG CCTCTCCTTG TAGTCTTGTG
    AATGAGACCA CTTTGATTAA ATACTCCAGG CTGCCAACCA TAAACAAGCA TAGTTTCCGG
    TACTTTGTCT TGGATAACAG TGTCATCCTG GCAATGCTGG AACAACCTCT TGGAAATGAG
    CAGAATGATT TTTTCCCCTC TGTCACTGTG CTGGTCCGGG GAATGTCTGG AAGACTTGCT
    TGGGCACAAC AGCTTTGTCT TTTACCCAGA GGAGCAAAAG CAAATCAGAA GCTTTTTGTA
    CCTGAACCTC GCCCAGTTCC TAAAAATGAC GTTGGATTTA AATATTCTGT GAAACATCGG
    CCATTTCCTG AAGAGGTGGA CAAGATTCCT TTTGTGAAAG CAGATCTCAG CATTCCAGAT
    TTGCATGAAA TAGTCACTGA AGAATTAGAA GAGAGACACG AAAAATTAAG GAGTGGCATG
    GCCCAGCAGA TTGCTTATGA AATACACCTT GAGCAACAGA GTGAGGAGGA ATTGCAGAAG
    AGAAGTTTTC CTGACCCAGT TACGGATTGC AAGCCCCCGC CTCCTGCCCA GGAATTCCAA
    ACAGCCCGCC TTTTTCTCTC ACACTTTGGA TTTTTGTCCT TAGAAGCACT GAAGGAACCT
    GCAAATAGTC GTCTACCTCC TCACCTTATT GCACTTGATT CCACGATACC TGGATTTTTT
    GATGACATTG GGTATCTGGA TCTCTTGCCA TGTCGTCCTT TTGACACAGT TTTTATTTTC
    TATATGAAGC CAGGTCAGAA AACGAACCAA GAGATTTTAA AGAATGTGGA GTCTTCCAGA
    ACTGTTCAGC CACATTTCCT AGAATTTTTG CTTTCCCTTG GCTGGTCAGT AGATGTGGGC
    AGACACCCTG GTTGGACTGG GCATGTTTCT ACCAGTTGGT CTATTAATTG TTGTGATGAT
    GGTGAAGGAT CTCAACAAGA AGAAGTGATT TCCTCTGAAG ATATTGGAGC TAGCATTTTC
    AATGGACAGA AGAAGGTGCT GTATTATGCT GATGCCCTTA CAGAAATTGC TTTTGTGGTT
    CCTTCTCCTG TGGAGTCCTT AACTGATTCA TTGGAAAGTA ACATCTCGGA CCAAGATAGT
    GATTCAAATA TGGATCTTAT GCCAGGAATT CTGAAACAGC CATCCCTGAC ACTTGAGCTT
    TTCCCCAATC ATACAGACAA TCTTAATTCC TCACAGAGGC TCAGTCCCAG TTCCAGAATG
    AGGAAGCTGC CTCAGGGTCG CCCTGTTCCT CCCCTTGGAC CTGAGACAAG AGTTTCTGTA
    GTCTGGGTGG AACGCTATGA TGATATAGAA AACTTTCCCC TCTCAGAGCT GATGACAGAG
    ATCAGTACTG GTGTGGAAAC TACTGCAAAT AGTAGCACTT CACTGAGATC TACAACTCTT
    GAAAAAGAAG TTCCTGTCAT CTTCATCCAC CCTTTAAACA CTGGATTATT CCGGATAAAA
    ATTCAAGGAG CCACTGGAAA ATTTAATATG GTCATCCCTC TTGTGGATGG GATGATTGTC
    AGCAGGCGAG CTCTTGGCTT TCTGGTGAGG CAGACTGTAA TTAACATTTG TAGAAGAAAG
    AGACTGGAAA GTGACTCCTA CAGTCCCCCC CATGTCCGCC GGAAACAGAA AATCACCGAC
    ATTGTCAACA AGTACCGGAA CAAGCAGCTG GAGCCAGAGT TTTATACTTC ACTTTTCCAG
    GAGGTTGGAC TCAAGAACTG CAGTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93081
    Clone ID Related Accession (Same CDS sequence) XM_017027969.2 , XM_017027969.1
    Accession Version XM_017027969.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017027969.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGGAGGA AGGAGCCTCA GTACCTGAGG AACAGACAGG AACAGGGTTC CAGTCAGATT 
    CGACTATGCT TACAGGTCCT GAGAGCCATT CAGAAACTGG CCCGTGAGTC ATCTCTCATG
    GCCCGAGAAA CTTGGGAAGT CTTACTGTTG TTTCTTCTGC AGATTAACGA CATACTTCTG
    GCCCCACCAA CTGTTCAAGG TGGCATTGCT GAGAATCTAG CAGAGAAGTT GATTGGTGTT
    CTCTTTGAGG TGTGGTTACT AGCTTGTACT CGGTGCTTCC CAACACCTCC TTATTGGAAA
    ACAGCCAAGG AGATGGTGGC TAACTGGAGG CATCACCCAG CAGTGGTGGA GCAGTGGAGC
    AAGGTCATTT GTGCACTCAC TTCCAGATTG CTACGCTTTA CATATGGTCC TTCATTTCCT
    GCATTTAAAG TTCCCGATGA AGATGCCAGT CTGATCCCTC CAGAAATGGA TAATGAGTGT
    GTTGCACAGA CATGGTTTCG CTTTTTACAC ATGTTAAGTA ATCCTGTGGA TTTGAGTAAC
    CCAGCTATTA TAAGCTCTAC TCCCAAATTT CAGGAACAGT TCTTGAATGT GAGCGGAATG
    CCGCAAGAAT TGAATCAGTA TCCCTGCCTT AAACATCTGC CTCAAATATT TTTTCGTGCC
    ATGCGTGGAA TCAGCTGTCT GGTGGATGCA TTCTTAGGTA TTTCTAGACC CCGATCAGAC
    AGTGCTCCCC CAACACCCGT GAATAGATTA AGTATGCCTC AAAGTGCTGC TGTCAGTACC
    ACCCCCCCAC ATAACCGGAG GCACCGGGCT GTTACTGTGA ATAAGGCCAC CATGAAGACA
    AGCACAGTTA GTACTGCTCA TGCCTCTAAA GTTCAGCACC AGACGTCCTC CACCTCTCCT
    CTGTCAAGTC CAAATCAGAC TAGTTCAGAA CCCCGGCCAC TGCCTGCCCC TCGGAGACCA
    AAGGTTAACA GCATCTTGAA TCTCTTTGGA TCATGGTTAT TTGATGCAGC ATTTGTTCAC
    TGTAAACTTC ATAATGGGAT AAACAGAGAC AGCAGCATGA CTGCCATTAC AACACAAGCT
    AGCATGGAGT TTCGACGGAA AGGGTCACAA ATGTCCACAG ACACCATGGT TTCCAATCCT
    ATGTTTGATG CAAGTGAATT TCCTGATAAC TATGAAGCAG GAAGAGCTGA GGCTTGTGGG
    ACACTGTGTA GGATTTTTTG TAGCAAGAAG ACTGGAGAAG AGATTCTGCC AGCTTATTTA
    TCCAGATTTT ACATGCTTTT AATTCAAGGT TTGCAGATAA ATGATTATGT GTGCCATCCT
    GTCTTGGCCA GCGTTATTCT AAACTCTCCT CCTTTGTTCT GCTGTGACTT GAAAGGGATT
    GATGTTGTGG TTCCTTACTT TATTTCAGCT CTTGAAACCA TTTTGCCTGA CAGAGAACTC
    TCAAAATTCA AAAGCTATGT AAATCCAACA GAATTGCGAA GATCCTCCAT TAATATCCTG
    CTTTCTTTGT TGCCCCTCCC TCATCATTTT GGCACAGTCA AATCTGAGGT GGTCCTGGAA
    GGAAAGTTTA GTAACGATGA CAGCTCTTCT TATGATAAAC CAATAACTTT TCTGTCCCTG
    AAGTTGAGAC TTGTGAATAT ATTAATAGGT GCCTTGCAAA CTGAAACGGA CCCCAACAAC
    ACCCAAATGA TATTAGGGGC AATGTTAAAT ATTGTTCAAG ATTCAGCACT TTTGGAAGCC
    ATTGGTTGCC AGATGGAGAT GGGTGGTGGA GAAAATAACC TGAAGAGTCA TAGTCGCACC
    AATAGTGGTA TTAGTTCAGC AAGTGGTGGA AGCACGGAGC CCACGACTCC CGATAGTGAG
    AGACCTGCTC AAGCTCTCTT AAGAGATTAT GATTCAGCTG CTGGGCTCCT GATTCGCAGC
    ATTCATCTCG TCACCCAAAG ACTCAACTCC CAGTGGCGCC AAGACATGAG CATATCACTG
    GCAGCTCTAG AGCTCCTCTC TGGCCTTGCA AAGGTAAAAG TGATGGTTGA CTCAGGAGAC
    CGGAAGCGAG CCATCAGTTC TGTGTGCACC TACATTGTTT ATCAGTGTAG TCGGCCAGCT
    CCTTTACACT CCAGGGATCT GCACTCCATG ATAGTGGCAG CTTTTCAGTG TCTCTGTGTC
    TGGCTGACAG AGCACCCTGA TATGCTTGAT GAAAAGGACT GCCTTAAGGA AGTACTGGAG
    ATTGTGGAAC TGGGTATCTC AGGAAGTAAG TCCAAGAACA ATGAGCAAGA GGTCAAGTAC
    AAAGGAGATA AGGAGCCAAA CCCTGCATCT ATGAGGGTAA AGGATGCTGC TGAAGCCACC
    CTAACATGCA TTATGCAGTT GCTCGGCGCA TTTCCTTCAC CTAGTGGTCC TGCCTCTCCT
    TGTAGTCTTG TGAATGAGAC CACTTTGATT AAATACTCCA GGCTGCCAAC CATAAACAAG
    CATAGTTTCC GGTACTTTGT CTTGGATAAC AGTGTCATCC TGGCAATGCT GGAACAACCT
    CTTGGAAATG AGCAGAATGA TTTTTTCCCC TCTGTCACTG TGCTGGTCCG GGGAATGTCT
    GGAAGACTTG CTTGGGCACA ACAGCTTTGT CTTTTACCCA GAGGAGCAAA AGCAAATCAG
    AAGCTTTTTG TACCTGAACC TCGCCCAGTT CCTAAAAATG ACGTTGGATT TAAATATTCT
    GTGAAACATC GGCCATTTCC TGAAGAGGTG GACAAGATTC CTTTTGTGAA AGCAGATCTC
    AGCATTCCAG ATTTGCATGA AATAGTCACT GAAGAATTAG AAGAGAGACA CGAAAAATTA
    AGGAGTGGCA TGGCCCAGCA GATTGCTTAT GAAATACACC TTGAGCAACA GAGTGAGGAG
    GAATTGCAGA AGAGAAGTTT TCCTGACCCA GTTACGGATT GCAAGCCCCC GCCTCCTGCC
    CAGGAATTCC AAACAGCCCG CCTTTTTCTC TCACACTTTG GATTTTTGTC CTTAGAAGCA
    CTGAAGGAAC CTGCAAATAG TCGTCTACCT CCTCACCTTA TTGCACTTGA TTCCACGATA
    CCTGGATTTT TTGATGACAT TGGGTATCTG GATCTCTTGC CATGTCGTCC TTTTGACACA
    GTTTTTATTT TCTATATGAA GCCAGGTCAG AAAACGAACC AAGAGATTTT AAAGAATGTG
    GAGTCTTCCA GAACTGTTCA GCCACATTTC CTAGAATTTT TGCTTTCCCT TGGCTGGTCA
    GTAGATGTGG GCAGACACCC TGGTTGGACT GGGCATGTTT CTACCAGTTG GTCTATTAAT
    TGTTGTGATG ATGGTGAAGG ATCTCAACAA GAAGTGATTT CCTCTGAAGA TATTGGAGCT
    AGCATTTTCA ATGGACAGAA GAAGGTGCTG TATTATGCTG ATGCCCTTAC AGAAATTGCT
    TTTGTGGTTC CTTCTCCTGT GGAGTCCTTA ACTGATTCAT TGGAAAGTAA CATCTCGGAC
    CAAGATAGTG ATTCAAATAT GGATCTTATG CCAGGAATTC TGAAACAGCC ATCCCTGACA
    CTTGAGCTTT TCCCCAATCA TACAGACAAT CTTAATTCCT CACAGAGGCT CAGTCCCAGT
    TCCAGAATGA GGAAGCTGCC TCAGGGTCGC CCTGTTCCTC CCCTTGGACC TGAGACAAGA
    GTTTCTGTAG TCTGGGTGGA ACGCTATGAT GATATAGAAA ACTTTCCCCT CTCAGAGCTG
    ATGACAGAGA TCAGTACTGG TGTGGAAACT ACTGCAAATA GTAGCACTTC ACTGAGATCT
    ACAACTCTTG AAAAAGAAGT TCCTGTCATC TTCATCCACC CTTTAAACAC TGGATTATTC
    CGGATAAAAA TTCAAGGAGC CACTGGAAAA TTTAATATGG TCATCCCTCT TGTGGATGGG
    ATGATTGTCA GCAGGCGAGC TCTTGGCTTT CTGGTGAGGC AGACTGTAAT TAACATTTGT
    AGAAGAAAGA GACTGGAAAG TGACTCCTAC AGTCCCCCCC ATGTCCGCCG GAAACAGAAA
    ATCACCGACA TTGTCAACAA GTACCGGAAC AAGCAGCTGG AGCCAGAGTT TTATACTTCA
    CTTTTCCAGG AGGTTGGACT CAAGAACTGC AGTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883458.1
    CDS672..4790
    Translation

    Target ORF information:

    RefSeq Version XM_017027969.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027969.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGGAGGA AGGAGCCTCA GTACCTGAGG AACAGACAGG AACAGGGTTC CAGTCAGATT 
    CGACTATGCT TACAGGTCCT GAGAGCCATT CAGAAACTGG CCCGTGAGTC ATCTCTCATG
    GCCCGAGAAA CTTGGGAAGT CTTACTGTTG TTTCTTCTGC AGATTAACGA CATACTTCTG
    GCCCCACCAA CTGTTCAAGG TGGCATTGCT GAGAATCTAG CAGAGAAGTT GATTGGTGTT
    CTCTTTGAGG TGTGGTTACT AGCTTGTACT CGGTGCTTCC CAACACCTCC TTATTGGAAA
    ACAGCCAAGG AGATGGTGGC TAACTGGAGG CATCACCCAG CAGTGGTGGA GCAGTGGAGC
    AAGGTCATTT GTGCACTCAC TTCCAGATTG CTACGCTTTA CATATGGTCC TTCATTTCCT
    GCATTTAAAG TTCCCGATGA AGATGCCAGT CTGATCCCTC CAGAAATGGA TAATGAGTGT
    GTTGCACAGA CATGGTTTCG CTTTTTACAC ATGTTAAGTA ATCCTGTGGA TTTGAGTAAC
    CCAGCTATTA TAAGCTCTAC TCCCAAATTT CAGGAACAGT TCTTGAATGT GAGCGGAATG
    CCGCAAGAAT TGAATCAGTA TCCCTGCCTT AAACATCTGC CTCAAATATT TTTTCGTGCC
    ATGCGTGGAA TCAGCTGTCT GGTGGATGCA TTCTTAGGTA TTTCTAGACC CCGATCAGAC
    AGTGCTCCCC CAACACCCGT GAATAGATTA AGTATGCCTC AAAGTGCTGC TGTCAGTACC
    ACCCCCCCAC ATAACCGGAG GCACCGGGCT GTTACTGTGA ATAAGGCCAC CATGAAGACA
    AGCACAGTTA GTACTGCTCA TGCCTCTAAA GTTCAGCACC AGACGTCCTC CACCTCTCCT
    CTGTCAAGTC CAAATCAGAC TAGTTCAGAA CCCCGGCCAC TGCCTGCCCC TCGGAGACCA
    AAGGTTAACA GCATCTTGAA TCTCTTTGGA TCATGGTTAT TTGATGCAGC ATTTGTTCAC
    TGTAAACTTC ATAATGGGAT AAACAGAGAC AGCAGCATGA CTGCCATTAC AACACAAGCT
    AGCATGGAGT TTCGACGGAA AGGGTCACAA ATGTCCACAG ACACCATGGT TTCCAATCCT
    ATGTTTGATG CAAGTGAATT TCCTGATAAC TATGAAGCAG GAAGAGCTGA GGCTTGTGGG
    ACACTGTGTA GGATTTTTTG TAGCAAGAAG ACTGGAGAAG AGATTCTGCC AGCTTATTTA
    TCCAGATTTT ACATGCTTTT AATTCAAGGT TTGCAGATAA ATGATTATGT GTGCCATCCT
    GTCTTGGCCA GCGTTATTCT AAACTCTCCT CCTTTGTTCT GCTGTGACTT GAAAGGGATT
    GATGTTGTGG TTCCTTACTT TATTTCAGCT CTTGAAACCA TTTTGCCTGA CAGAGAACTC
    TCAAAATTCA AAAGCTATGT AAATCCAACA GAATTGCGAA GATCCTCCAT TAATATCCTG
    CTTTCTTTGT TGCCCCTCCC TCATCATTTT GGCACAGTCA AATCTGAGGT GGTCCTGGAA
    GGAAAGTTTA GTAACGATGA CAGCTCTTCT TATGATAAAC CAATAACTTT TCTGTCCCTG
    AAGTTGAGAC TTGTGAATAT ATTAATAGGT GCCTTGCAAA CTGAAACGGA CCCCAACAAC
    ACCCAAATGA TATTAGGGGC AATGTTAAAT ATTGTTCAAG ATTCAGCACT TTTGGAAGCC
    ATTGGTTGCC AGATGGAGAT GGGTGGTGGA GAAAATAACC TGAAGAGTCA TAGTCGCACC
    AATAGTGGTA TTAGTTCAGC AAGTGGTGGA AGCACGGAGC CCACGACTCC CGATAGTGAG
    AGACCTGCTC AAGCTCTCTT AAGAGATTAT GATTCAGCTG CTGGGCTCCT GATTCGCAGC
    ATTCATCTCG TCACCCAAAG ACTCAACTCC CAGTGGCGCC AAGACATGAG CATATCACTG
    GCAGCTCTAG AGCTCCTCTC TGGCCTTGCA AAGGTAAAAG TGATGGTTGA CTCAGGAGAC
    CGGAAGCGAG CCATCAGTTC TGTGTGCACC TACATTGTTT ATCAGTGTAG TCGGCCAGCT
    CCTTTACACT CCAGGGATCT GCACTCCATG ATAGTGGCAG CTTTTCAGTG TCTCTGTGTC
    TGGCTGACAG AGCACCCTGA TATGCTTGAT GAAAAGGACT GCCTTAAGGA AGTACTGGAG
    ATTGTGGAAC TGGGTATCTC AGGAAGTAAG TCCAAGAACA ATGAGCAAGA GGTCAAGTAC
    AAAGGAGATA AGGAGCCAAA CCCTGCATCT ATGAGGGTAA AGGATGCTGC TGAAGCCACC
    CTAACATGCA TTATGCAGTT GCTCGGCGCA TTTCCTTCAC CTAGTGGTCC TGCCTCTCCT
    TGTAGTCTTG TGAATGAGAC CACTTTGATT AAATACTCCA GGCTGCCAAC CATAAACAAG
    CATAGTTTCC GGTACTTTGT CTTGGATAAC AGTGTCATCC TGGCAATGCT GGAACAACCT
    CTTGGAAATG AGCAGAATGA TTTTTTCCCC TCTGTCACTG TGCTGGTCCG GGGAATGTCT
    GGAAGACTTG CTTGGGCACA ACAGCTTTGT CTTTTACCCA GAGGAGCAAA AGCAAATCAG
    AAGCTTTTTG TACCTGAACC TCGCCCAGTT CCTAAAAATG ACGTTGGATT TAAATATTCT
    GTGAAACATC GGCCATTTCC TGAAGAGGTG GACAAGATTC CTTTTGTGAA AGCAGATCTC
    AGCATTCCAG ATTTGCATGA AATAGTCACT GAAGAATTAG AAGAGAGACA CGAAAAATTA
    AGGAGTGGCA TGGCCCAGCA GATTGCTTAT GAAATACACC TTGAGCAACA GAGTGAGGAG
    GAATTGCAGA AGAGAAGTTT TCCTGACCCA GTTACGGATT GCAAGCCCCC GCCTCCTGCC
    CAGGAATTCC AAACAGCCCG CCTTTTTCTC TCACACTTTG GATTTTTGTC CTTAGAAGCA
    CTGAAGGAAC CTGCAAATAG TCGTCTACCT CCTCACCTTA TTGCACTTGA TTCCACGATA
    CCTGGATTTT TTGATGACAT TGGGTATCTG GATCTCTTGC CATGTCGTCC TTTTGACACA
    GTTTTTATTT TCTATATGAA GCCAGGTCAG AAAACGAACC AAGAGATTTT AAAGAATGTG
    GAGTCTTCCA GAACTGTTCA GCCACATTTC CTAGAATTTT TGCTTTCCCT TGGCTGGTCA
    GTAGATGTGG GCAGACACCC TGGTTGGACT GGGCATGTTT CTACCAGTTG GTCTATTAAT
    TGTTGTGATG ATGGTGAAGG ATCTCAACAA GAAGTGATTT CCTCTGAAGA TATTGGAGCT
    AGCATTTTCA ATGGACAGAA GAAGGTGCTG TATTATGCTG ATGCCCTTAC AGAAATTGCT
    TTTGTGGTTC CTTCTCCTGT GGAGTCCTTA ACTGATTCAT TGGAAAGTAA CATCTCGGAC
    CAAGATAGTG ATTCAAATAT GGATCTTATG CCAGGAATTC TGAAACAGCC ATCCCTGACA
    CTTGAGCTTT TCCCCAATCA TACAGACAAT CTTAATTCCT CACAGAGGCT CAGTCCCAGT
    TCCAGAATGA GGAAGCTGCC TCAGGGTCGC CCTGTTCCTC CCCTTGGACC TGAGACAAGA
    GTTTCTGTAG TCTGGGTGGA ACGCTATGAT GATATAGAAA ACTTTCCCCT CTCAGAGCTG
    ATGACAGAGA TCAGTACTGG TGTGGAAACT ACTGCAAATA GTAGCACTTC ACTGAGATCT
    ACAACTCTTG AAAAAGAAGT TCCTGTCATC TTCATCCACC CTTTAAACAC TGGATTATTC
    CGGATAAAAA TTCAAGGAGC CACTGGAAAA TTTAATATGG TCATCCCTCT TGTGGATGGG
    ATGATTGTCA GCAGGCGAGC TCTTGGCTTT CTGGTGAGGC AGACTGTAAT TAACATTTGT
    AGAAGAAAGA GACTGGAAAG TGACTCCTAC AGTCCCCCCC ATGTCCGCCG GAAACAGAAA
    ATCACCGACA TTGTCAACAA GTACCGGAAC AAGCAGCTGG AGCCAGAGTT TTATACTTCA
    CTTTTCCAGG AGGTTGGACT CAAGAACTGC AGTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93081
    Clone ID Related Accession (Same CDS sequence) XM_017027969.2 , XM_017027969.1
    Accession Version XM_017027969.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4119bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGGAGGA AGGAGCCTCA GTACCTGAGG AACAGACAGG AACAGGGTTC CAGTCAGATT 
    CGACTATGCT TACAGGTCCT GAGAGCCATT CAGAAACTGG CCCGTGAGTC ATCTCTCATG
    GCCCGAGAAA CTTGGGAAGT CTTACTGTTG TTTCTTCTGC AGATTAACGA CATACTTCTG
    GCCCCACCAA CTGTTCAAGG TGGCATTGCT GAGAATCTAG CAGAGAAGTT GATTGGTGTT
    CTCTTTGAGG TGTGGTTACT AGCTTGTACT CGGTGCTTCC CAACACCTCC TTATTGGAAA
    ACAGCCAAGG AGATGGTGGC TAACTGGAGG CATCACCCAG CAGTGGTGGA GCAGTGGAGC
    AAGGTCATTT GTGCACTCAC TTCCAGATTG CTACGCTTTA CATATGGTCC TTCATTTCCT
    GCATTTAAAG TTCCCGATGA AGATGCCAGT CTGATCCCTC CAGAAATGGA TAATGAGTGT
    GTTGCACAGA CATGGTTTCG CTTTTTACAC ATGTTAAGTA ATCCTGTGGA TTTGAGTAAC
    CCAGCTATTA TAAGCTCTAC TCCCAAATTT CAGGAACAGT TCTTGAATGT GAGCGGAATG
    CCGCAAGAAT TGAATCAGTA TCCCTGCCTT AAACATCTGC CTCAAATATT TTTTCGTGCC
    ATGCGTGGAA TCAGCTGTCT GGTGGATGCA TTCTTAGGTA TTTCTAGACC CCGATCAGAC
    AGTGCTCCCC CAACACCCGT GAATAGATTA AGTATGCCTC AAAGTGCTGC TGTCAGTACC
    ACCCCCCCAC ATAACCGGAG GCACCGGGCT GTTACTGTGA ATAAGGCCAC CATGAAGACA
    AGCACAGTTA GTACTGCTCA TGCCTCTAAA GTTCAGCACC AGACGTCCTC CACCTCTCCT
    CTGTCAAGTC CAAATCAGAC TAGTTCAGAA CCCCGGCCAC TGCCTGCCCC TCGGAGACCA
    AAGGTTAACA GCATCTTGAA TCTCTTTGGA TCATGGTTAT TTGATGCAGC ATTTGTTCAC
    TGTAAACTTC ATAATGGGAT AAACAGAGAC AGCAGCATGA CTGCCATTAC AACACAAGCT
    AGCATGGAGT TTCGACGGAA AGGGTCACAA ATGTCCACAG ACACCATGGT TTCCAATCCT
    ATGTTTGATG CAAGTGAATT TCCTGATAAC TATGAAGCAG GAAGAGCTGA GGCTTGTGGG
    ACACTGTGTA GGATTTTTTG TAGCAAGAAG ACTGGAGAAG AGATTCTGCC AGCTTATTTA
    TCCAGATTTT ACATGCTTTT AATTCAAGGT TTGCAGATAA ATGATTATGT GTGCCATCCT
    GTCTTGGCCA GCGTTATTCT AAACTCTCCT CCTTTGTTCT GCTGTGACTT GAAAGGGATT
    GATGTTGTGG TTCCTTACTT TATTTCAGCT CTTGAAACCA TTTTGCCTGA CAGAGAACTC
    TCAAAATTCA AAAGCTATGT AAATCCAACA GAATTGCGAA GATCCTCCAT TAATATCCTG
    CTTTCTTTGT TGCCCCTCCC TCATCATTTT GGCACAGTCA AATCTGAGGT GGTCCTGGAA
    GGAAAGTTTA GTAACGATGA CAGCTCTTCT TATGATAAAC CAATAACTTT TCTGTCCCTG
    AAGTTGAGAC TTGTGAATAT ATTAATAGGT GCCTTGCAAA CTGAAACGGA CCCCAACAAC
    ACCCAAATGA TATTAGGGGC AATGTTAAAT ATTGTTCAAG ATTCAGCACT TTTGGAAGCC
    ATTGGTTGCC AGATGGAGAT GGGTGGTGGA GAAAATAACC TGAAGAGTCA TAGTCGCACC
    AATAGTGGTA TTAGTTCAGC AAGTGGTGGA AGCACGGAGC CCACGACTCC CGATAGTGAG
    AGACCTGCTC AAGCTCTCTT AAGAGATTAT GATTCAGCTG CTGGGCTCCT GATTCGCAGC
    ATTCATCTCG TCACCCAAAG ACTCAACTCC CAGTGGCGCC AAGACATGAG CATATCACTG
    GCAGCTCTAG AGCTCCTCTC TGGCCTTGCA AAGGTAAAAG TGATGGTTGA CTCAGGAGAC
    CGGAAGCGAG CCATCAGTTC TGTGTGCACC TACATTGTTT ATCAGTGTAG TCGGCCAGCT
    CCTTTACACT CCAGGGATCT GCACTCCATG ATAGTGGCAG CTTTTCAGTG TCTCTGTGTC
    TGGCTGACAG AGCACCCTGA TATGCTTGAT GAAAAGGACT GCCTTAAGGA AGTACTGGAG
    ATTGTGGAAC TGGGTATCTC AGGAAGTAAG TCCAAGAACA ATGAGCAAGA GGTCAAGTAC
    AAAGGAGATA AGGAGCCAAA CCCTGCATCT ATGAGGGTAA AGGATGCTGC TGAAGCCACC
    CTAACATGCA TTATGCAGTT GCTCGGCGCA TTTCCTTCAC CTAGTGGTCC TGCCTCTCCT
    TGTAGTCTTG TGAATGAGAC CACTTTGATT AAATACTCCA GGCTGCCAAC CATAAACAAG
    CATAGTTTCC GGTACTTTGT CTTGGATAAC AGTGTCATCC TGGCAATGCT GGAACAACCT
    CTTGGAAATG AGCAGAATGA TTTTTTCCCC TCTGTCACTG TGCTGGTCCG GGGAATGTCT
    GGAAGACTTG CTTGGGCACA ACAGCTTTGT CTTTTACCCA GAGGAGCAAA AGCAAATCAG
    AAGCTTTTTG TACCTGAACC TCGCCCAGTT CCTAAAAATG ACGTTGGATT TAAATATTCT
    GTGAAACATC GGCCATTTCC TGAAGAGGTG GACAAGATTC CTTTTGTGAA AGCAGATCTC
    AGCATTCCAG ATTTGCATGA AATAGTCACT GAAGAATTAG AAGAGAGACA CGAAAAATTA
    AGGAGTGGCA TGGCCCAGCA GATTGCTTAT GAAATACACC TTGAGCAACA GAGTGAGGAG
    GAATTGCAGA AGAGAAGTTT TCCTGACCCA GTTACGGATT GCAAGCCCCC GCCTCCTGCC
    CAGGAATTCC AAACAGCCCG CCTTTTTCTC TCACACTTTG GATTTTTGTC CTTAGAAGCA
    CTGAAGGAAC CTGCAAATAG TCGTCTACCT CCTCACCTTA TTGCACTTGA TTCCACGATA
    CCTGGATTTT TTGATGACAT TGGGTATCTG GATCTCTTGC CATGTCGTCC TTTTGACACA
    GTTTTTATTT TCTATATGAA GCCAGGTCAG AAAACGAACC AAGAGATTTT AAAGAATGTG
    GAGTCTTCCA GAACTGTTCA GCCACATTTC CTAGAATTTT TGCTTTCCCT TGGCTGGTCA
    GTAGATGTGG GCAGACACCC TGGTTGGACT GGGCATGTTT CTACCAGTTG GTCTATTAAT
    TGTTGTGATG ATGGTGAAGG ATCTCAACAA GAAGTGATTT CCTCTGAAGA TATTGGAGCT
    AGCATTTTCA ATGGACAGAA GAAGGTGCTG TATTATGCTG ATGCCCTTAC AGAAATTGCT
    TTTGTGGTTC CTTCTCCTGT GGAGTCCTTA ACTGATTCAT TGGAAAGTAA CATCTCGGAC
    CAAGATAGTG ATTCAAATAT GGATCTTATG CCAGGAATTC TGAAACAGCC ATCCCTGACA
    CTTGAGCTTT TCCCCAATCA TACAGACAAT CTTAATTCCT CACAGAGGCT CAGTCCCAGT
    TCCAGAATGA GGAAGCTGCC TCAGGGTCGC CCTGTTCCTC CCCTTGGACC TGAGACAAGA
    GTTTCTGTAG TCTGGGTGGA ACGCTATGAT GATATAGAAA ACTTTCCCCT CTCAGAGCTG
    ATGACAGAGA TCAGTACTGG TGTGGAAACT ACTGCAAATA GTAGCACTTC ACTGAGATCT
    ACAACTCTTG AAAAAGAAGT TCCTGTCATC TTCATCCACC CTTTAAACAC TGGATTATTC
    CGGATAAAAA TTCAAGGAGC CACTGGAAAA TTTAATATGG TCATCCCTCT TGTGGATGGG
    ATGATTGTCA GCAGGCGAGC TCTTGGCTTT CTGGTGAGGC AGACTGTAAT TAACATTTGT
    AGAAGAAAGA GACTGGAAAG TGACTCCTAC AGTCCCCCCC ATGTCCGCCG GAAACAGAAA
    ATCACCGACA TTGTCAACAA GTACCGGAAC AAGCAGCTGG AGCCAGAGTT TTATACTTCA
    CTTTTCCAGG AGGTTGGACT CAAGAACTGC AGTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883458.1
    CDS672..4790
    Translation

    Target ORF information:

    RefSeq Version XM_017027969.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027969.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGAGGAGGA AGGAGCCTCA GTACCTGAGG AACAGACAGG AACAGGGTTC CAGTCAGATT 
    CGACTATGCT TACAGGTCCT GAGAGCCATT CAGAAACTGG CCCGTGAGTC ATCTCTCATG
    GCCCGAGAAA CTTGGGAAGT CTTACTGTTG TTTCTTCTGC AGATTAACGA CATACTTCTG
    GCCCCACCAA CTGTTCAAGG TGGCATTGCT GAGAATCTAG CAGAGAAGTT GATTGGTGTT
    CTCTTTGAGG TGTGGTTACT AGCTTGTACT CGGTGCTTCC CAACACCTCC TTATTGGAAA
    ACAGCCAAGG AGATGGTGGC TAACTGGAGG CATCACCCAG CAGTGGTGGA GCAGTGGAGC
    AAGGTCATTT GTGCACTCAC TTCCAGATTG CTACGCTTTA CATATGGTCC TTCATTTCCT
    GCATTTAAAG TTCCCGATGA AGATGCCAGT CTGATCCCTC CAGAAATGGA TAATGAGTGT
    GTTGCACAGA CATGGTTTCG CTTTTTACAC ATGTTAAGTA ATCCTGTGGA TTTGAGTAAC
    CCAGCTATTA TAAGCTCTAC TCCCAAATTT CAGGAACAGT TCTTGAATGT GAGCGGAATG
    CCGCAAGAAT TGAATCAGTA TCCCTGCCTT AAACATCTGC CTCAAATATT TTTTCGTGCC
    ATGCGTGGAA TCAGCTGTCT GGTGGATGCA TTCTTAGGTA TTTCTAGACC CCGATCAGAC
    AGTGCTCCCC CAACACCCGT GAATAGATTA AGTATGCCTC AAAGTGCTGC TGTCAGTACC
    ACCCCCCCAC ATAACCGGAG GCACCGGGCT GTTACTGTGA ATAAGGCCAC CATGAAGACA
    AGCACAGTTA GTACTGCTCA TGCCTCTAAA GTTCAGCACC AGACGTCCTC CACCTCTCCT
    CTGTCAAGTC CAAATCAGAC TAGTTCAGAA CCCCGGCCAC TGCCTGCCCC TCGGAGACCA
    AAGGTTAACA GCATCTTGAA TCTCTTTGGA TCATGGTTAT TTGATGCAGC ATTTGTTCAC
    TGTAAACTTC ATAATGGGAT AAACAGAGAC AGCAGCATGA CTGCCATTAC AACACAAGCT
    AGCATGGAGT TTCGACGGAA AGGGTCACAA ATGTCCACAG ACACCATGGT TTCCAATCCT
    ATGTTTGATG CAAGTGAATT TCCTGATAAC TATGAAGCAG GAAGAGCTGA GGCTTGTGGG
    ACACTGTGTA GGATTTTTTG TAGCAAGAAG ACTGGAGAAG AGATTCTGCC AGCTTATTTA
    TCCAGATTTT ACATGCTTTT AATTCAAGGT TTGCAGATAA ATGATTATGT GTGCCATCCT
    GTCTTGGCCA GCGTTATTCT AAACTCTCCT CCTTTGTTCT GCTGTGACTT GAAAGGGATT
    GATGTTGTGG TTCCTTACTT TATTTCAGCT CTTGAAACCA TTTTGCCTGA CAGAGAACTC
    TCAAAATTCA AAAGCTATGT AAATCCAACA GAATTGCGAA GATCCTCCAT TAATATCCTG
    CTTTCTTTGT TGCCCCTCCC TCATCATTTT GGCACAGTCA AATCTGAGGT GGTCCTGGAA
    GGAAAGTTTA GTAACGATGA CAGCTCTTCT TATGATAAAC CAATAACTTT TCTGTCCCTG
    AAGTTGAGAC TTGTGAATAT ATTAATAGGT GCCTTGCAAA CTGAAACGGA CCCCAACAAC
    ACCCAAATGA TATTAGGGGC AATGTTAAAT ATTGTTCAAG ATTCAGCACT TTTGGAAGCC
    ATTGGTTGCC AGATGGAGAT GGGTGGTGGA GAAAATAACC TGAAGAGTCA TAGTCGCACC
    AATAGTGGTA TTAGTTCAGC AAGTGGTGGA AGCACGGAGC CCACGACTCC CGATAGTGAG
    AGACCTGCTC AAGCTCTCTT AAGAGATTAT GATTCAGCTG CTGGGCTCCT GATTCGCAGC
    ATTCATCTCG TCACCCAAAG ACTCAACTCC CAGTGGCGCC AAGACATGAG CATATCACTG
    GCAGCTCTAG AGCTCCTCTC TGGCCTTGCA AAGGTAAAAG TGATGGTTGA CTCAGGAGAC
    CGGAAGCGAG CCATCAGTTC TGTGTGCACC TACATTGTTT ATCAGTGTAG TCGGCCAGCT
    CCTTTACACT CCAGGGATCT GCACTCCATG ATAGTGGCAG CTTTTCAGTG TCTCTGTGTC
    TGGCTGACAG AGCACCCTGA TATGCTTGAT GAAAAGGACT GCCTTAAGGA AGTACTGGAG
    ATTGTGGAAC TGGGTATCTC AGGAAGTAAG TCCAAGAACA ATGAGCAAGA GGTCAAGTAC
    AAAGGAGATA AGGAGCCAAA CCCTGCATCT ATGAGGGTAA AGGATGCTGC TGAAGCCACC
    CTAACATGCA TTATGCAGTT GCTCGGCGCA TTTCCTTCAC CTAGTGGTCC TGCCTCTCCT
    TGTAGTCTTG TGAATGAGAC CACTTTGATT AAATACTCCA GGCTGCCAAC CATAAACAAG
    CATAGTTTCC GGTACTTTGT CTTGGATAAC AGTGTCATCC TGGCAATGCT GGAACAACCT
    CTTGGAAATG AGCAGAATGA TTTTTTCCCC TCTGTCACTG TGCTGGTCCG GGGAATGTCT
    GGAAGACTTG CTTGGGCACA ACAGCTTTGT CTTTTACCCA GAGGAGCAAA AGCAAATCAG
    AAGCTTTTTG TACCTGAACC TCGCCCAGTT CCTAAAAATG ACGTTGGATT TAAATATTCT
    GTGAAACATC GGCCATTTCC TGAAGAGGTG GACAAGATTC CTTTTGTGAA AGCAGATCTC
    AGCATTCCAG ATTTGCATGA AATAGTCACT GAAGAATTAG AAGAGAGACA CGAAAAATTA
    AGGAGTGGCA TGGCCCAGCA GATTGCTTAT GAAATACACC TTGAGCAACA GAGTGAGGAG
    GAATTGCAGA AGAGAAGTTT TCCTGACCCA GTTACGGATT GCAAGCCCCC GCCTCCTGCC
    CAGGAATTCC AAACAGCCCG CCTTTTTCTC TCACACTTTG GATTTTTGTC CTTAGAAGCA
    CTGAAGGAAC CTGCAAATAG TCGTCTACCT CCTCACCTTA TTGCACTTGA TTCCACGATA
    CCTGGATTTT TTGATGACAT TGGGTATCTG GATCTCTTGC CATGTCGTCC TTTTGACACA
    GTTTTTATTT TCTATATGAA GCCAGGTCAG AAAACGAACC AAGAGATTTT AAAGAATGTG
    GAGTCTTCCA GAACTGTTCA GCCACATTTC CTAGAATTTT TGCTTTCCCT TGGCTGGTCA
    GTAGATGTGG GCAGACACCC TGGTTGGACT GGGCATGTTT CTACCAGTTG GTCTATTAAT
    TGTTGTGATG ATGGTGAAGG ATCTCAACAA GAAGTGATTT CCTCTGAAGA TATTGGAGCT
    AGCATTTTCA ATGGACAGAA GAAGGTGCTG TATTATGCTG ATGCCCTTAC AGAAATTGCT
    TTTGTGGTTC CTTCTCCTGT GGAGTCCTTA ACTGATTCAT TGGAAAGTAA CATCTCGGAC
    CAAGATAGTG ATTCAAATAT GGATCTTATG CCAGGAATTC TGAAACAGCC ATCCCTGACA
    CTTGAGCTTT TCCCCAATCA TACAGACAAT CTTAATTCCT CACAGAGGCT CAGTCCCAGT
    TCCAGAATGA GGAAGCTGCC TCAGGGTCGC CCTGTTCCTC CCCTTGGACC TGAGACAAGA
    GTTTCTGTAG TCTGGGTGGA ACGCTATGAT GATATAGAAA ACTTTCCCCT CTCAGAGCTG
    ATGACAGAGA TCAGTACTGG TGTGGAAACT ACTGCAAATA GTAGCACTTC ACTGAGATCT
    ACAACTCTTG AAAAAGAAGT TCCTGTCATC TTCATCCACC CTTTAAACAC TGGATTATTC
    CGGATAAAAA TTCAAGGAGC CACTGGAAAA TTTAATATGG TCATCCCTCT TGTGGATGGG
    ATGATTGTCA GCAGGCGAGC TCTTGGCTTT CTGGTGAGGC AGACTGTAAT TAACATTTGT
    AGAAGAAAGA GACTGGAAAG TGACTCCTAC AGTCCCCCCC ATGTCCGCCG GAAACAGAAA
    ATCACCGACA TTGTCAACAA GTACCGGAAC AAGCAGCTGG AGCCAGAGTT TTATACTTCA
    CTTTTCCAGG AGGTTGGACT CAAGAACTGC AGTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93078
    Clone ID Related Accession (Same CDS sequence) XM_017027966.2 , XM_017027966.1
    Accession Version XM_017027966.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4509bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017027966.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GATTCAGCTG CTGGGCTCCT GATTCGCAGC ATTCATCTCG TCACCCAAAG ACTCAACTCC
    CAGTGGCGCC AAGACATGAG CATATCACTG GCAGCTCTAG AGCTCCTCTC TGGCCTTGCA
    AAGGTAAAAG TGATGGTTGA CTCAGGAGAC CGGAAGCGAG CCATCAGTTC TGTGTGCACC
    TACATTGTTT ATCAGTGTAG TCGGCCAGCT CCTTTACACT CCAGGGATCT GCACTCCATG
    ATAGTGGCAG CTTTTCAGTG TCTCTGTGTC TGGCTGACAG AGCACCCTGA TATGCTTGAT
    GAAAAGGACT GCCTTAAGGA AGTACTGGAG ATTGTGGAAC TGGGTATCTC AGGAAGTAAG
    TCCAAGAACA ATGAGCAAGA GGTCAAGTAC AAAGGAGATA AGGAGCCAAA CCCTGCATCT
    ATGAGGGTAA AGGATGCTGC TGAAGCCACC CTAACATGCA TTATGCAGTT GCTCGGCGCA
    TTTCCTTCAC CTAGTGGTCC TGCCTCTCCT TGTAGTCTTG TGAATGAGAC CACTTTGATT
    AAATACTCCA GGCTGCCAAC CATAAACAAG CATAGTTTCC GGTACTTTGT CTTGGATAAC
    AGTGTCATCC TGGCAATGCT GGAACAACCT CTTGGAAATG AGCAGAATGA TTTTTTCCCC
    TCTGTCACTG TGCTGGTCCG GGGAATGTCT GGAAGACTTG CTTGGGCACA ACAGCTTTGT
    CTTTTACCCA GAGGAGCAAA AGCAAATCAG AAGCTTTTTG TACCTGAACC TCGCCCAGTT
    CCTAAAAATG ACGTTGGATT TAAATATTCT GTGAAACATC GGCCATTTCC TGAAGAGGTG
    GACAAGATTC CTTTTGTGAA AGCAGATCTC AGCATTCCAG ATTTGCATGA AATAGTCACT
    GAAGAATTAG AAGAGAGACA CGAAAAATTA AGGAGTGGCA TGGCCCAGCA GATTGCTTAT
    GAAATACACC TTGAGCAACA GAGTGAGGAG GAATTGCAGA AGAGAAGTTT TCCTGACCCA
    GTTACGGATT GCAAGCCCCC GCCTCCTGCC CAGGAATTCC AAACAGCCCG CCTTTTTCTC
    TCACACTTTG GATTTTTGTC CTTAGAAGCA CTGAAGGAAC CTGCAAATAG TCGTCTACCT
    CCTCACCTTA TTGCACTTGA TTCCACGATA CCTGGATTTT TTGATGACAT TGGGTATCTG
    GATCTCTTGC CATGTCGTCC TTTTGACACA GTTTTTATTT TCTATATGAA GCCAGGTCAG
    AAAACGAACC AAGAGATTTT AAAGAATGTG GAGTCTTCCA GAACTGTTCA GCCACATTTC
    CTAGAATTTT TGCTTTCCCT TGGCTGGTCA GTAGATGTGG GCAGACACCC TGGTTGGACT
    GGGCATGTTT CTACCAGTTG GTCTATTAAT TGTTGTGATG ATGGTGAAGG ATCTCAACAA
    GAAGTGATTT CCTCTGAAGA TATTGGAGCT AGCATTTTCA ATGGACAGAA GAAGGTGCTG
    TATTATGCTG ATGCCCTTAC AGAAATTGCT TTTGTGGTTC CTTCTCCTGT GGAGTCCTTA
    ACTGATTCAT TGGAAAGTAA CATCTCGGAC CAAGATAGTG ATTCAAATAT GGATCTTATG
    CCAGGAATTC TGAAACAGCC ATCCCTGACA CTTGAGCTTT TCCCCAATCA TACAGACAAT
    CTTAATTCCT CACAGAGGCT CAGTCCCAGT TCCAGAATGA GGAAGCTGCC TCAGGGTCGC
    CCTGTTCCTC CCCTTGGACC TGAGACAAGA GTTTCTGTAG TCTGGGTGGA ACGCTATGAT
    GATATAGAAA ACTTTCCCCT CTCAGAGCTG ATGACAGAGA TCAGTACTGG TGTGGAAACT
    ACTGCAAATA GTAGCACTTC ACTGAGATCT ACAACTCTTG AAAAAGAAGT TCCTGTCATC
    TTCATCCACC CTTTAAACAC TGGATTATTC CGGATAAAAA TTCAAGGAGC CACTGGAAAA
    TTTAATATGG TCATCCCTCT TGTGGATGGG ATGATTGTCA GCAGGCGAGC TCTTGGCTTT
    CTGGTGAGGC AGACTGTAAT TAACATTTGT AGAAGAAAGA GACTGGAAAG TGACTCCTAC
    AGTCCCCCCC ATGTCCGCCG GAAACAGAAA ATCACCGACA TTGTCAACAA GTACCGGAAC
    AAGCAGCTGG AGCCAGAGTT TTATACTTCA CTTTTCCAGG AGGTTGGACT CAAGAACTGC
    AGTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883455.1
    CDS281..4789
    Translation

    Target ORF information:

    RefSeq Version XM_017027966.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027966.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GATTCAGCTG CTGGGCTCCT GATTCGCAGC ATTCATCTCG TCACCCAAAG ACTCAACTCC
    CAGTGGCGCC AAGACATGAG CATATCACTG GCAGCTCTAG AGCTCCTCTC TGGCCTTGCA
    AAGGTAAAAG TGATGGTTGA CTCAGGAGAC CGGAAGCGAG CCATCAGTTC TGTGTGCACC
    TACATTGTTT ATCAGTGTAG TCGGCCAGCT CCTTTACACT CCAGGGATCT GCACTCCATG
    ATAGTGGCAG CTTTTCAGTG TCTCTGTGTC TGGCTGACAG AGCACCCTGA TATGCTTGAT
    GAAAAGGACT GCCTTAAGGA AGTACTGGAG ATTGTGGAAC TGGGTATCTC AGGAAGTAAG
    TCCAAGAACA ATGAGCAAGA GGTCAAGTAC AAAGGAGATA AGGAGCCAAA CCCTGCATCT
    ATGAGGGTAA AGGATGCTGC TGAAGCCACC CTAACATGCA TTATGCAGTT GCTCGGCGCA
    TTTCCTTCAC CTAGTGGTCC TGCCTCTCCT TGTAGTCTTG TGAATGAGAC CACTTTGATT
    AAATACTCCA GGCTGCCAAC CATAAACAAG CATAGTTTCC GGTACTTTGT CTTGGATAAC
    AGTGTCATCC TGGCAATGCT GGAACAACCT CTTGGAAATG AGCAGAATGA TTTTTTCCCC
    TCTGTCACTG TGCTGGTCCG GGGAATGTCT GGAAGACTTG CTTGGGCACA ACAGCTTTGT
    CTTTTACCCA GAGGAGCAAA AGCAAATCAG AAGCTTTTTG TACCTGAACC TCGCCCAGTT
    CCTAAAAATG ACGTTGGATT TAAATATTCT GTGAAACATC GGCCATTTCC TGAAGAGGTG
    GACAAGATTC CTTTTGTGAA AGCAGATCTC AGCATTCCAG ATTTGCATGA AATAGTCACT
    GAAGAATTAG AAGAGAGACA CGAAAAATTA AGGAGTGGCA TGGCCCAGCA GATTGCTTAT
    GAAATACACC TTGAGCAACA GAGTGAGGAG GAATTGCAGA AGAGAAGTTT TCCTGACCCA
    GTTACGGATT GCAAGCCCCC GCCTCCTGCC CAGGAATTCC AAACAGCCCG CCTTTTTCTC
    TCACACTTTG GATTTTTGTC CTTAGAAGCA CTGAAGGAAC CTGCAAATAG TCGTCTACCT
    CCTCACCTTA TTGCACTTGA TTCCACGATA CCTGGATTTT TTGATGACAT TGGGTATCTG
    GATCTCTTGC CATGTCGTCC TTTTGACACA GTTTTTATTT TCTATATGAA GCCAGGTCAG
    AAAACGAACC AAGAGATTTT AAAGAATGTG GAGTCTTCCA GAACTGTTCA GCCACATTTC
    CTAGAATTTT TGCTTTCCCT TGGCTGGTCA GTAGATGTGG GCAGACACCC TGGTTGGACT
    GGGCATGTTT CTACCAGTTG GTCTATTAAT TGTTGTGATG ATGGTGAAGG ATCTCAACAA
    GAAGTGATTT CCTCTGAAGA TATTGGAGCT AGCATTTTCA ATGGACAGAA GAAGGTGCTG
    TATTATGCTG ATGCCCTTAC AGAAATTGCT TTTGTGGTTC CTTCTCCTGT GGAGTCCTTA
    ACTGATTCAT TGGAAAGTAA CATCTCGGAC CAAGATAGTG ATTCAAATAT GGATCTTATG
    CCAGGAATTC TGAAACAGCC ATCCCTGACA CTTGAGCTTT TCCCCAATCA TACAGACAAT
    CTTAATTCCT CACAGAGGCT CAGTCCCAGT TCCAGAATGA GGAAGCTGCC TCAGGGTCGC
    CCTGTTCCTC CCCTTGGACC TGAGACAAGA GTTTCTGTAG TCTGGGTGGA ACGCTATGAT
    GATATAGAAA ACTTTCCCCT CTCAGAGCTG ATGACAGAGA TCAGTACTGG TGTGGAAACT
    ACTGCAAATA GTAGCACTTC ACTGAGATCT ACAACTCTTG AAAAAGAAGT TCCTGTCATC
    TTCATCCACC CTTTAAACAC TGGATTATTC CGGATAAAAA TTCAAGGAGC CACTGGAAAA
    TTTAATATGG TCATCCCTCT TGTGGATGGG ATGATTGTCA GCAGGCGAGC TCTTGGCTTT
    CTGGTGAGGC AGACTGTAAT TAACATTTGT AGAAGAAAGA GACTGGAAAG TGACTCCTAC
    AGTCCCCCCC ATGTCCGCCG GAAACAGAAA ATCACCGACA TTGTCAACAA GTACCGGAAC
    AAGCAGCTGG AGCCAGAGTT TTATACTTCA CTTTTCCAGG AGGTTGGACT CAAGAACTGC
    AGTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu93078
    Clone ID Related Accession (Same CDS sequence) XM_017027966.2 , XM_017027966.1
    Accession Version XM_017027966.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4509bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GATTCAGCTG CTGGGCTCCT GATTCGCAGC ATTCATCTCG TCACCCAAAG ACTCAACTCC
    CAGTGGCGCC AAGACATGAG CATATCACTG GCAGCTCTAG AGCTCCTCTC TGGCCTTGCA
    AAGGTAAAAG TGATGGTTGA CTCAGGAGAC CGGAAGCGAG CCATCAGTTC TGTGTGCACC
    TACATTGTTT ATCAGTGTAG TCGGCCAGCT CCTTTACACT CCAGGGATCT GCACTCCATG
    ATAGTGGCAG CTTTTCAGTG TCTCTGTGTC TGGCTGACAG AGCACCCTGA TATGCTTGAT
    GAAAAGGACT GCCTTAAGGA AGTACTGGAG ATTGTGGAAC TGGGTATCTC AGGAAGTAAG
    TCCAAGAACA ATGAGCAAGA GGTCAAGTAC AAAGGAGATA AGGAGCCAAA CCCTGCATCT
    ATGAGGGTAA AGGATGCTGC TGAAGCCACC CTAACATGCA TTATGCAGTT GCTCGGCGCA
    TTTCCTTCAC CTAGTGGTCC TGCCTCTCCT TGTAGTCTTG TGAATGAGAC CACTTTGATT
    AAATACTCCA GGCTGCCAAC CATAAACAAG CATAGTTTCC GGTACTTTGT CTTGGATAAC
    AGTGTCATCC TGGCAATGCT GGAACAACCT CTTGGAAATG AGCAGAATGA TTTTTTCCCC
    TCTGTCACTG TGCTGGTCCG GGGAATGTCT GGAAGACTTG CTTGGGCACA ACAGCTTTGT
    CTTTTACCCA GAGGAGCAAA AGCAAATCAG AAGCTTTTTG TACCTGAACC TCGCCCAGTT
    CCTAAAAATG ACGTTGGATT TAAATATTCT GTGAAACATC GGCCATTTCC TGAAGAGGTG
    GACAAGATTC CTTTTGTGAA AGCAGATCTC AGCATTCCAG ATTTGCATGA AATAGTCACT
    GAAGAATTAG AAGAGAGACA CGAAAAATTA AGGAGTGGCA TGGCCCAGCA GATTGCTTAT
    GAAATACACC TTGAGCAACA GAGTGAGGAG GAATTGCAGA AGAGAAGTTT TCCTGACCCA
    GTTACGGATT GCAAGCCCCC GCCTCCTGCC CAGGAATTCC AAACAGCCCG CCTTTTTCTC
    TCACACTTTG GATTTTTGTC CTTAGAAGCA CTGAAGGAAC CTGCAAATAG TCGTCTACCT
    CCTCACCTTA TTGCACTTGA TTCCACGATA CCTGGATTTT TTGATGACAT TGGGTATCTG
    GATCTCTTGC CATGTCGTCC TTTTGACACA GTTTTTATTT TCTATATGAA GCCAGGTCAG
    AAAACGAACC AAGAGATTTT AAAGAATGTG GAGTCTTCCA GAACTGTTCA GCCACATTTC
    CTAGAATTTT TGCTTTCCCT TGGCTGGTCA GTAGATGTGG GCAGACACCC TGGTTGGACT
    GGGCATGTTT CTACCAGTTG GTCTATTAAT TGTTGTGATG ATGGTGAAGG ATCTCAACAA
    GAAGTGATTT CCTCTGAAGA TATTGGAGCT AGCATTTTCA ATGGACAGAA GAAGGTGCTG
    TATTATGCTG ATGCCCTTAC AGAAATTGCT TTTGTGGTTC CTTCTCCTGT GGAGTCCTTA
    ACTGATTCAT TGGAAAGTAA CATCTCGGAC CAAGATAGTG ATTCAAATAT GGATCTTATG
    CCAGGAATTC TGAAACAGCC ATCCCTGACA CTTGAGCTTT TCCCCAATCA TACAGACAAT
    CTTAATTCCT CACAGAGGCT CAGTCCCAGT TCCAGAATGA GGAAGCTGCC TCAGGGTCGC
    CCTGTTCCTC CCCTTGGACC TGAGACAAGA GTTTCTGTAG TCTGGGTGGA ACGCTATGAT
    GATATAGAAA ACTTTCCCCT CTCAGAGCTG ATGACAGAGA TCAGTACTGG TGTGGAAACT
    ACTGCAAATA GTAGCACTTC ACTGAGATCT ACAACTCTTG AAAAAGAAGT TCCTGTCATC
    TTCATCCACC CTTTAAACAC TGGATTATTC CGGATAAAAA TTCAAGGAGC CACTGGAAAA
    TTTAATATGG TCATCCCTCT TGTGGATGGG ATGATTGTCA GCAGGCGAGC TCTTGGCTTT
    CTGGTGAGGC AGACTGTAAT TAACATTTGT AGAAGAAAGA GACTGGAAAG TGACTCCTAC
    AGTCCCCCCC ATGTCCGCCG GAAACAGAAA ATCACCGACA TTGTCAACAA GTACCGGAAC
    AAGCAGCTGG AGCCAGAGTT TTATACTTCA CTTTTCCAGG AGGTTGGACT CAAGAACTGC
    AGTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016883455.1
    CDS253..4761
    Translation

    Target ORF information:

    RefSeq Version XM_017027966.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027966.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GATTCAGCTG CTGGGCTCCT GATTCGCAGC ATTCATCTCG TCACCCAAAG ACTCAACTCC
    CAGTGGCGCC AAGACATGAG CATATCACTG GCAGCTCTAG AGCTCCTCTC TGGCCTTGCA
    AAGGTAAAAG TGATGGTTGA CTCAGGAGAC CGGAAGCGAG CCATCAGTTC TGTGTGCACC
    TACATTGTTT ATCAGTGTAG TCGGCCAGCT CCTTTACACT CCAGGGATCT GCACTCCATG
    ATAGTGGCAG CTTTTCAGTG TCTCTGTGTC TGGCTGACAG AGCACCCTGA TATGCTTGAT
    GAAAAGGACT GCCTTAAGGA AGTACTGGAG ATTGTGGAAC TGGGTATCTC AGGAAGTAAG
    TCCAAGAACA ATGAGCAAGA GGTCAAGTAC AAAGGAGATA AGGAGCCAAA CCCTGCATCT
    ATGAGGGTAA AGGATGCTGC TGAAGCCACC CTAACATGCA TTATGCAGTT GCTCGGCGCA
    TTTCCTTCAC CTAGTGGTCC TGCCTCTCCT TGTAGTCTTG TGAATGAGAC CACTTTGATT
    AAATACTCCA GGCTGCCAAC CATAAACAAG CATAGTTTCC GGTACTTTGT CTTGGATAAC
    AGTGTCATCC TGGCAATGCT GGAACAACCT CTTGGAAATG AGCAGAATGA TTTTTTCCCC
    TCTGTCACTG TGCTGGTCCG GGGAATGTCT GGAAGACTTG CTTGGGCACA ACAGCTTTGT
    CTTTTACCCA GAGGAGCAAA AGCAAATCAG AAGCTTTTTG TACCTGAACC TCGCCCAGTT
    CCTAAAAATG ACGTTGGATT TAAATATTCT GTGAAACATC GGCCATTTCC TGAAGAGGTG
    GACAAGATTC CTTTTGTGAA AGCAGATCTC AGCATTCCAG ATTTGCATGA AATAGTCACT
    GAAGAATTAG AAGAGAGACA CGAAAAATTA AGGAGTGGCA TGGCCCAGCA GATTGCTTAT
    GAAATACACC TTGAGCAACA GAGTGAGGAG GAATTGCAGA AGAGAAGTTT TCCTGACCCA
    GTTACGGATT GCAAGCCCCC GCCTCCTGCC CAGGAATTCC AAACAGCCCG CCTTTTTCTC
    TCACACTTTG GATTTTTGTC CTTAGAAGCA CTGAAGGAAC CTGCAAATAG TCGTCTACCT
    CCTCACCTTA TTGCACTTGA TTCCACGATA CCTGGATTTT TTGATGACAT TGGGTATCTG
    GATCTCTTGC CATGTCGTCC TTTTGACACA GTTTTTATTT TCTATATGAA GCCAGGTCAG
    AAAACGAACC AAGAGATTTT AAAGAATGTG GAGTCTTCCA GAACTGTTCA GCCACATTTC
    CTAGAATTTT TGCTTTCCCT TGGCTGGTCA GTAGATGTGG GCAGACACCC TGGTTGGACT
    GGGCATGTTT CTACCAGTTG GTCTATTAAT TGTTGTGATG ATGGTGAAGG ATCTCAACAA
    GAAGTGATTT CCTCTGAAGA TATTGGAGCT AGCATTTTCA ATGGACAGAA GAAGGTGCTG
    TATTATGCTG ATGCCCTTAC AGAAATTGCT TTTGTGGTTC CTTCTCCTGT GGAGTCCTTA
    ACTGATTCAT TGGAAAGTAA CATCTCGGAC CAAGATAGTG ATTCAAATAT GGATCTTATG
    CCAGGAATTC TGAAACAGCC ATCCCTGACA CTTGAGCTTT TCCCCAATCA TACAGACAAT
    CTTAATTCCT CACAGAGGCT CAGTCCCAGT TCCAGAATGA GGAAGCTGCC TCAGGGTCGC
    CCTGTTCCTC CCCTTGGACC TGAGACAAGA GTTTCTGTAG TCTGGGTGGA ACGCTATGAT
    GATATAGAAA ACTTTCCCCT CTCAGAGCTG ATGACAGAGA TCAGTACTGG TGTGGAAACT
    ACTGCAAATA GTAGCACTTC ACTGAGATCT ACAACTCTTG AAAAAGAAGT TCCTGTCATC
    TTCATCCACC CTTTAAACAC TGGATTATTC CGGATAAAAA TTCAAGGAGC CACTGGAAAA
    TTTAATATGG TCATCCCTCT TGTGGATGGG ATGATTGTCA GCAGGCGAGC TCTTGGCTTT
    CTGGTGAGGC AGACTGTAAT TAACATTTGT AGAAGAAAGA GACTGGAAAG TGACTCCTAC
    AGTCCCCCCC ATGTCCGCCG GAAACAGAAA ATCACCGACA TTGTCAACAA GTACCGGAAC
    AAGCAGCTGG AGCCAGAGTT TTATACTTCA CTTTTCCAGG AGGTTGGACT CAAGAACTGC
    AGTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00394
    Clone ID Related Accession (Same CDS sequence) NM_001282917.1 , NM_001282917.2 , NM_020336.4 , NM_020336.3
    Accession Version NM_001282917.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-29
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR002520.1, AB033045.3, CR936800.1, BC047482.1, BX641080.1 and BE669526.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: CR936800.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC CCAAAGACTC AACTCCCAGT GGCGCCAAGA CATGAGCATA
    TCACTGGCAG CTCTAGAGCT CCTCTCTGGC CTTGCAAAGG TAAAAGTGAT GGTTGACTCA
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCACCTACA TTGTTTATCA GTGTAGTCGG
    CCAGCTCCTT TACACTCCAG GGATCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA CCCTGATATG CTTGATGAAA AGGACTGCCT TAAGGAAGTA
    CTGGAGATTG TGGAACTGGG TATCTCAGGA AGTAAGTCCA AGAACAATGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGCATTAT GCAGTTGCTC GGCGCATTTC CTTCACCTAG TGGTCCTGCC
    TCTCCTTGTA GTCTTGTGAA TGAGACCACT TTGATTAAAT ACTCCAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAA
    CAACCTCTTG GAAATGAGCA GAATGATTTT TTCCCCTCTG TCACTGTGCT GGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAG CTTTGTCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGC CCAGTTCCTA AAAATGACGT TGGATTTAAA
    TATTCTGTGA AACATCGGCC ATTTCCTGAA GAGGTGGACA AGATTCCTTT TGTGAAAGCA
    GATCTCAGCA TTCCAGATTT GCATGAAATA GTCACTGAAG AATTAGAAGA GAGACACGAA
    AAATTAAGGA GTGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA GCAACAGAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GACCCAGTTA CGGATTGCAA GCCCCCGCCT
    CCTGCCCAGG AATTCCAAAC AGCCCGCCTT TTTCTCTCAC ACTTTGGATT TTTGTCCTTA
    GAAGCACTGA AGGAACCTGC AAATAGTCGT CTACCTCCTC ACCTTATTGC ACTTGATTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCTTTT
    GACACAGTTT TTATTTTCTA TATGAAGCCA GGTCAGAAAA CGAACCAAGA GATTTTAAAG
    AATGTGGAGT CTTCCAGAAC TGTTCAGCCA CATTTCCTAG AATTTTTGCT TTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCTGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAATTGTT GTGATGATGG TGAAGGATCT CAACAAGAAG TGATTTCCTC TGAAGATATT
    GGAGCTAGCA TTTTCAATGG ACAGAAGAAG GTGCTGTATT ATGCTGATGC CCTTACAGAA
    ATTGCTTTTG TGGTTCCTTC TCCTGTGGAG TCCTTAACTG ATTCATTGGA AAGTAACATC
    TCGGACCAAG ATAGTGATTC AAATATGGAT CTTATGCCAG GAATTCTGAA ACAGCCATCC
    CTGACACTTG AGCTTTTCCC CAATCATACA GACAATCTTA ATTCCTCACA GAGGCTCAGT
    CCCAGTTCCA GAATGAGGAA GCTGCCTCAG GGTCGCCCTG TTCCTCCCCT TGGACCTGAG
    ACAAGAGTTT CTGTAGTCTG GGTGGAACGC TATGATGATA TAGAAAACTT TCCCCTCTCA
    GAGCTGATGA CAGAGATCAG TACTGGTGTG GAAACTACTG CAAATAGTAG CACTTCACTG
    AGATCTACAA CTCTTGAAAA AGAAGTTCCT GTCATCTTCA TCCACCCTTT AAACACTGGA
    TTATTCCGGA TAAAAATTCA AGGAGCCACT GGAAAATTTA ATATGGTCAT CCCTCTTGTG
    GATGGGATGA TTGTCAGCAG GCGAGCTCTT GGCTTTCTGG TGAGGCAGAC TGTAATTAAC
    ATTTGTAGAA GAAAGAGACT GGAAAGTGAC TCCTACAGTC CCCCCCATGT CCGCCGGAAA
    CAGAAAATCA CCGACATTGT CAACAAGTAC CGGAACAAGC AGCTGGAGCC AGAGTTTTAT
    ACTTCACTTT TCCAGGAGGT TGGACTCAAG AACTGCAGTT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269846.1
    CDS153..4637
    Translation

    Target ORF information:

    RefSeq Version NM_001282917.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282917.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC CCAAAGACTC AACTCCCAGT GGCGCCAAGA CATGAGCATA
    TCACTGGCAG CTCTAGAGCT CCTCTCTGGC CTTGCAAAGG TAAAAGTGAT GGTTGACTCA
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCACCTACA TTGTTTATCA GTGTAGTCGG
    CCAGCTCCTT TACACTCCAG GGATCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA CCCTGATATG CTTGATGAAA AGGACTGCCT TAAGGAAGTA
    CTGGAGATTG TGGAACTGGG TATCTCAGGA AGTAAGTCCA AGAACAATGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGCATTAT GCAGTTGCTC GGCGCATTTC CTTCACCTAG TGGTCCTGCC
    TCTCCTTGTA GTCTTGTGAA TGAGACCACT TTGATTAAAT ACTCCAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAA
    CAACCTCTTG GAAATGAGCA GAATGATTTT TTCCCCTCTG TCACTGTGCT GGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAG CTTTGTCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGC CCAGTTCCTA AAAATGACGT TGGATTTAAA
    TATTCTGTGA AACATCGGCC ATTTCCTGAA GAGGTGGACA AGATTCCTTT TGTGAAAGCA
    GATCTCAGCA TTCCAGATTT GCATGAAATA GTCACTGAAG AATTAGAAGA GAGACACGAA
    AAATTAAGGA GTGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA GCAACAGAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GACCCAGTTA CGGATTGCAA GCCCCCGCCT
    CCTGCCCAGG AATTCCAAAC AGCCCGCCTT TTTCTCTCAC ACTTTGGATT TTTGTCCTTA
    GAAGCACTGA AGGAACCTGC AAATAGTCGT CTACCTCCTC ACCTTATTGC ACTTGATTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCTTTT
    GACACAGTTT TTATTTTCTA TATGAAGCCA GGTCAGAAAA CGAACCAAGA GATTTTAAAG
    AATGTGGAGT CTTCCAGAAC TGTTCAGCCA CATTTCCTAG AATTTTTGCT TTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCTGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAATTGTT GTGATGATGG TGAAGGATCT CAACAAGAAG TGATTTCCTC TGAAGATATT
    GGAGCTAGCA TTTTCAATGG ACAGAAGAAG GTGCTGTATT ATGCTGATGC CCTTACAGAA
    ATTGCTTTTG TGGTTCCTTC TCCTGTGGAG TCCTTAACTG ATTCATTGGA AAGTAACATC
    TCGGACCAAG ATAGTGATTC AAATATGGAT CTTATGCCAG GAATTCTGAA ACAGCCATCC
    CTGACACTTG AGCTTTTCCC CAATCATACA GACAATCTTA ATTCCTCACA GAGGCTCAGT
    CCCAGTTCCA GAATGAGGAA GCTGCCTCAG GGTCGCCCTG TTCCTCCCCT TGGACCTGAG
    ACAAGAGTTT CTGTAGTCTG GGTGGAACGC TATGATGATA TAGAAAACTT TCCCCTCTCA
    GAGCTGATGA CAGAGATCAG TACTGGTGTG GAAACTACTG CAAATAGTAG CACTTCACTG
    AGATCTACAA CTCTTGAAAA AGAAGTTCCT GTCATCTTCA TCCACCCTTT AAACACTGGA
    TTATTCCGGA TAAAAATTCA AGGAGCCACT GGAAAATTTA ATATGGTCAT CCCTCTTGTG
    GATGGGATGA TTGTCAGCAG GCGAGCTCTT GGCTTTCTGG TGAGGCAGAC TGTAATTAAC
    ATTTGTAGAA GAAAGAGACT GGAAAGTGAC TCCTACAGTC CCCCCCATGT CCGCCGGAAA
    CAGAAAATCA CCGACATTGT CAACAAGTAC CGGAACAAGC AGCTGGAGCC AGAGTTTTAT
    ACTTCACTTT TCCAGGAGGT TGGACTCAAG AACTGCAGTT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00394
    Clone ID Related Accession (Same CDS sequence) NM_001282917.1 , NM_001282917.2 , NM_020336.4 , NM_020336.3
    Accession Version NM_001282917.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL049868.20 and AL035419.12. On May 31, 2019 this sequence version replaced NM_001282917.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: CR936800.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC CCAAAGACTC AACTCCCAGT GGCGCCAAGA CATGAGCATA
    TCACTGGCAG CTCTAGAGCT CCTCTCTGGC CTTGCAAAGG TAAAAGTGAT GGTTGACTCA
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCACCTACA TTGTTTATCA GTGTAGTCGG
    CCAGCTCCTT TACACTCCAG GGATCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA CCCTGATATG CTTGATGAAA AGGACTGCCT TAAGGAAGTA
    CTGGAGATTG TGGAACTGGG TATCTCAGGA AGTAAGTCCA AGAACAATGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGCATTAT GCAGTTGCTC GGCGCATTTC CTTCACCTAG TGGTCCTGCC
    TCTCCTTGTA GTCTTGTGAA TGAGACCACT TTGATTAAAT ACTCCAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAA
    CAACCTCTTG GAAATGAGCA GAATGATTTT TTCCCCTCTG TCACTGTGCT GGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAG CTTTGTCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGC CCAGTTCCTA AAAATGACGT TGGATTTAAA
    TATTCTGTGA AACATCGGCC ATTTCCTGAA GAGGTGGACA AGATTCCTTT TGTGAAAGCA
    GATCTCAGCA TTCCAGATTT GCATGAAATA GTCACTGAAG AATTAGAAGA GAGACACGAA
    AAATTAAGGA GTGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA GCAACAGAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GACCCAGTTA CGGATTGCAA GCCCCCGCCT
    CCTGCCCAGG AATTCCAAAC AGCCCGCCTT TTTCTCTCAC ACTTTGGATT TTTGTCCTTA
    GAAGCACTGA AGGAACCTGC AAATAGTCGT CTACCTCCTC ACCTTATTGC ACTTGATTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCTTTT
    GACACAGTTT TTATTTTCTA TATGAAGCCA GGTCAGAAAA CGAACCAAGA GATTTTAAAG
    AATGTGGAGT CTTCCAGAAC TGTTCAGCCA CATTTCCTAG AATTTTTGCT TTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCTGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAATTGTT GTGATGATGG TGAAGGATCT CAACAAGAAG TGATTTCCTC TGAAGATATT
    GGAGCTAGCA TTTTCAATGG ACAGAAGAAG GTGCTGTATT ATGCTGATGC CCTTACAGAA
    ATTGCTTTTG TGGTTCCTTC TCCTGTGGAG TCCTTAACTG ATTCATTGGA AAGTAACATC
    TCGGACCAAG ATAGTGATTC AAATATGGAT CTTATGCCAG GAATTCTGAA ACAGCCATCC
    CTGACACTTG AGCTTTTCCC CAATCATACA GACAATCTTA ATTCCTCACA GAGGCTCAGT
    CCCAGTTCCA GAATGAGGAA GCTGCCTCAG GGTCGCCCTG TTCCTCCCCT TGGACCTGAG
    ACAAGAGTTT CTGTAGTCTG GGTGGAACGC TATGATGATA TAGAAAACTT TCCCCTCTCA
    GAGCTGATGA CAGAGATCAG TACTGGTGTG GAAACTACTG CAAATAGTAG CACTTCACTG
    AGATCTACAA CTCTTGAAAA AGAAGTTCCT GTCATCTTCA TCCACCCTTT AAACACTGGA
    TTATTCCGGA TAAAAATTCA AGGAGCCACT GGAAAATTTA ATATGGTCAT CCCTCTTGTG
    GATGGGATGA TTGTCAGCAG GCGAGCTCTT GGCTTTCTGG TGAGGCAGAC TGTAATTAAC
    ATTTGTAGAA GAAAGAGACT GGAAAGTGAC TCCTACAGTC CCCCCCATGT CCGCCGGAAA
    CAGAAAATCA CCGACATTGT CAACAAGTAC CGGAACAAGC AGCTGGAGCC AGAGTTTTAT
    ACTTCACTTT TCCAGGAGGT TGGACTCAAG AACTGCAGTT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269846.1
    CDS116..4600
    Translation

    Target ORF information:

    RefSeq Version NM_001282917.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282917.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC CCAAAGACTC AACTCCCAGT GGCGCCAAGA CATGAGCATA
    TCACTGGCAG CTCTAGAGCT CCTCTCTGGC CTTGCAAAGG TAAAAGTGAT GGTTGACTCA
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCACCTACA TTGTTTATCA GTGTAGTCGG
    CCAGCTCCTT TACACTCCAG GGATCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA CCCTGATATG CTTGATGAAA AGGACTGCCT TAAGGAAGTA
    CTGGAGATTG TGGAACTGGG TATCTCAGGA AGTAAGTCCA AGAACAATGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGCATTAT GCAGTTGCTC GGCGCATTTC CTTCACCTAG TGGTCCTGCC
    TCTCCTTGTA GTCTTGTGAA TGAGACCACT TTGATTAAAT ACTCCAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAA
    CAACCTCTTG GAAATGAGCA GAATGATTTT TTCCCCTCTG TCACTGTGCT GGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAG CTTTGTCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGC CCAGTTCCTA AAAATGACGT TGGATTTAAA
    TATTCTGTGA AACATCGGCC ATTTCCTGAA GAGGTGGACA AGATTCCTTT TGTGAAAGCA
    GATCTCAGCA TTCCAGATTT GCATGAAATA GTCACTGAAG AATTAGAAGA GAGACACGAA
    AAATTAAGGA GTGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA GCAACAGAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GACCCAGTTA CGGATTGCAA GCCCCCGCCT
    CCTGCCCAGG AATTCCAAAC AGCCCGCCTT TTTCTCTCAC ACTTTGGATT TTTGTCCTTA
    GAAGCACTGA AGGAACCTGC AAATAGTCGT CTACCTCCTC ACCTTATTGC ACTTGATTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCTTTT
    GACACAGTTT TTATTTTCTA TATGAAGCCA GGTCAGAAAA CGAACCAAGA GATTTTAAAG
    AATGTGGAGT CTTCCAGAAC TGTTCAGCCA CATTTCCTAG AATTTTTGCT TTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCTGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAATTGTT GTGATGATGG TGAAGGATCT CAACAAGAAG TGATTTCCTC TGAAGATATT
    GGAGCTAGCA TTTTCAATGG ACAGAAGAAG GTGCTGTATT ATGCTGATGC CCTTACAGAA
    ATTGCTTTTG TGGTTCCTTC TCCTGTGGAG TCCTTAACTG ATTCATTGGA AAGTAACATC
    TCGGACCAAG ATAGTGATTC AAATATGGAT CTTATGCCAG GAATTCTGAA ACAGCCATCC
    CTGACACTTG AGCTTTTCCC CAATCATACA GACAATCTTA ATTCCTCACA GAGGCTCAGT
    CCCAGTTCCA GAATGAGGAA GCTGCCTCAG GGTCGCCCTG TTCCTCCCCT TGGACCTGAG
    ACAAGAGTTT CTGTAGTCTG GGTGGAACGC TATGATGATA TAGAAAACTT TCCCCTCTCA
    GAGCTGATGA CAGAGATCAG TACTGGTGTG GAAACTACTG CAAATAGTAG CACTTCACTG
    AGATCTACAA CTCTTGAAAA AGAAGTTCCT GTCATCTTCA TCCACCCTTT AAACACTGGA
    TTATTCCGGA TAAAAATTCA AGGAGCCACT GGAAAATTTA ATATGGTCAT CCCTCTTGTG
    GATGGGATGA TTGTCAGCAG GCGAGCTCTT GGCTTTCTGG TGAGGCAGAC TGTAATTAAC
    ATTTGTAGAA GAAAGAGACT GGAAAGTGAC TCCTACAGTC CCCCCCATGT CCGCCGGAAA
    CAGAAAATCA CCGACATTGT CAACAAGTAC CGGAACAAGC AGCTGGAGCC AGAGTTTTAT
    ACTTCACTTT TCCAGGAGGT TGGACTCAAG AACTGCAGTT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00394
    Clone ID Related Accession (Same CDS sequence) NM_001282917.1 , NM_001282917.2 , NM_020336.4 , NM_020336.3
    Accession Version NM_020336.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-31
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL049868.20 and AL035419.12. On Nov 23, 2018 this sequence version replaced NM_020336.3. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## Transcript exon combination :: BC047482.1, SRR1803611.2929.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262879.11/ ENSP00000262879.6 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC CCAAAGACTC AACTCCCAGT GGCGCCAAGA CATGAGCATA
    TCACTGGCAG CTCTAGAGCT CCTCTCTGGC CTTGCAAAGG TAAAAGTGAT GGTTGACTCA
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCACCTACA TTGTTTATCA GTGTAGTCGG
    CCAGCTCCTT TACACTCCAG GGATCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA CCCTGATATG CTTGATGAAA AGGACTGCCT TAAGGAAGTA
    CTGGAGATTG TGGAACTGGG TATCTCAGGA AGTAAGTCCA AGAACAATGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGCATTAT GCAGTTGCTC GGCGCATTTC CTTCACCTAG TGGTCCTGCC
    TCTCCTTGTA GTCTTGTGAA TGAGACCACT TTGATTAAAT ACTCCAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAA
    CAACCTCTTG GAAATGAGCA GAATGATTTT TTCCCCTCTG TCACTGTGCT GGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAG CTTTGTCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGC CCAGTTCCTA AAAATGACGT TGGATTTAAA
    TATTCTGTGA AACATCGGCC ATTTCCTGAA GAGGTGGACA AGATTCCTTT TGTGAAAGCA
    GATCTCAGCA TTCCAGATTT GCATGAAATA GTCACTGAAG AATTAGAAGA GAGACACGAA
    AAATTAAGGA GTGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA GCAACAGAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GACCCAGTTA CGGATTGCAA GCCCCCGCCT
    CCTGCCCAGG AATTCCAAAC AGCCCGCCTT TTTCTCTCAC ACTTTGGATT TTTGTCCTTA
    GAAGCACTGA AGGAACCTGC AAATAGTCGT CTACCTCCTC ACCTTATTGC ACTTGATTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCTTTT
    GACACAGTTT TTATTTTCTA TATGAAGCCA GGTCAGAAAA CGAACCAAGA GATTTTAAAG
    AATGTGGAGT CTTCCAGAAC TGTTCAGCCA CATTTCCTAG AATTTTTGCT TTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCTGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAATTGTT GTGATGATGG TGAAGGATCT CAACAAGAAG TGATTTCCTC TGAAGATATT
    GGAGCTAGCA TTTTCAATGG ACAGAAGAAG GTGCTGTATT ATGCTGATGC CCTTACAGAA
    ATTGCTTTTG TGGTTCCTTC TCCTGTGGAG TCCTTAACTG ATTCATTGGA AAGTAACATC
    TCGGACCAAG ATAGTGATTC AAATATGGAT CTTATGCCAG GAATTCTGAA ACAGCCATCC
    CTGACACTTG AGCTTTTCCC CAATCATACA GACAATCTTA ATTCCTCACA GAGGCTCAGT
    CCCAGTTCCA GAATGAGGAA GCTGCCTCAG GGTCGCCCTG TTCCTCCCCT TGGACCTGAG
    ACAAGAGTTT CTGTAGTCTG GGTGGAACGC TATGATGATA TAGAAAACTT TCCCCTCTCA
    GAGCTGATGA CAGAGATCAG TACTGGTGTG GAAACTACTG CAAATAGTAG CACTTCACTG
    AGATCTACAA CTCTTGAAAA AGAAGTTCCT GTCATCTTCA TCCACCCTTT AAACACTGGA
    TTATTCCGGA TAAAAATTCA AGGAGCCACT GGAAAATTTA ATATGGTCAT CCCTCTTGTG
    GATGGGATGA TTGTCAGCAG GCGAGCTCTT GGCTTTCTGG TGAGGCAGAC TGTAATTAAC
    ATTTGTAGAA GAAAGAGACT GGAAAGTGAC TCCTACAGTC CCCCCCATGT CCGCCGGAAA
    CAGAAAATCA CCGACATTGT CAACAAGTAC CGGAACAAGC AGCTGGAGCC AGAGTTTTAT
    ACTTCACTTT TCCAGGAGGT TGGACTCAAG AACTGCAGTT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065069.1
    CDS258..4742
    Translation

    Target ORF information:

    RefSeq Version NM_020336.4
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020336.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC CCAAAGACTC AACTCCCAGT GGCGCCAAGA CATGAGCATA
    TCACTGGCAG CTCTAGAGCT CCTCTCTGGC CTTGCAAAGG TAAAAGTGAT GGTTGACTCA
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCACCTACA TTGTTTATCA GTGTAGTCGG
    CCAGCTCCTT TACACTCCAG GGATCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA CCCTGATATG CTTGATGAAA AGGACTGCCT TAAGGAAGTA
    CTGGAGATTG TGGAACTGGG TATCTCAGGA AGTAAGTCCA AGAACAATGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGCATTAT GCAGTTGCTC GGCGCATTTC CTTCACCTAG TGGTCCTGCC
    TCTCCTTGTA GTCTTGTGAA TGAGACCACT TTGATTAAAT ACTCCAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAA
    CAACCTCTTG GAAATGAGCA GAATGATTTT TTCCCCTCTG TCACTGTGCT GGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAG CTTTGTCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGC CCAGTTCCTA AAAATGACGT TGGATTTAAA
    TATTCTGTGA AACATCGGCC ATTTCCTGAA GAGGTGGACA AGATTCCTTT TGTGAAAGCA
    GATCTCAGCA TTCCAGATTT GCATGAAATA GTCACTGAAG AATTAGAAGA GAGACACGAA
    AAATTAAGGA GTGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA GCAACAGAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GACCCAGTTA CGGATTGCAA GCCCCCGCCT
    CCTGCCCAGG AATTCCAAAC AGCCCGCCTT TTTCTCTCAC ACTTTGGATT TTTGTCCTTA
    GAAGCACTGA AGGAACCTGC AAATAGTCGT CTACCTCCTC ACCTTATTGC ACTTGATTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCTTTT
    GACACAGTTT TTATTTTCTA TATGAAGCCA GGTCAGAAAA CGAACCAAGA GATTTTAAAG
    AATGTGGAGT CTTCCAGAAC TGTTCAGCCA CATTTCCTAG AATTTTTGCT TTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCTGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAATTGTT GTGATGATGG TGAAGGATCT CAACAAGAAG TGATTTCCTC TGAAGATATT
    GGAGCTAGCA TTTTCAATGG ACAGAAGAAG GTGCTGTATT ATGCTGATGC CCTTACAGAA
    ATTGCTTTTG TGGTTCCTTC TCCTGTGGAG TCCTTAACTG ATTCATTGGA AAGTAACATC
    TCGGACCAAG ATAGTGATTC AAATATGGAT CTTATGCCAG GAATTCTGAA ACAGCCATCC
    CTGACACTTG AGCTTTTCCC CAATCATACA GACAATCTTA ATTCCTCACA GAGGCTCAGT
    CCCAGTTCCA GAATGAGGAA GCTGCCTCAG GGTCGCCCTG TTCCTCCCCT TGGACCTGAG
    ACAAGAGTTT CTGTAGTCTG GGTGGAACGC TATGATGATA TAGAAAACTT TCCCCTCTCA
    GAGCTGATGA CAGAGATCAG TACTGGTGTG GAAACTACTG CAAATAGTAG CACTTCACTG
    AGATCTACAA CTCTTGAAAA AGAAGTTCCT GTCATCTTCA TCCACCCTTT AAACACTGGA
    TTATTCCGGA TAAAAATTCA AGGAGCCACT GGAAAATTTA ATATGGTCAT CCCTCTTGTG
    GATGGGATGA TTGTCAGCAG GCGAGCTCTT GGCTTTCTGG TGAGGCAGAC TGTAATTAAC
    ATTTGTAGAA GAAAGAGACT GGAAAGTGAC TCCTACAGTC CCCCCCATGT CCGCCGGAAA
    CAGAAAATCA CCGACATTGT CAACAAGTAC CGGAACAAGC AGCTGGAGCC AGAGTTTTAT
    ACTTCACTTT TCCAGGAGGT TGGACTCAAG AACTGCAGTT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu00394
    Clone ID Related Accession (Same CDS sequence) NM_001282917.1 , NM_001282917.2 , NM_020336.4 , NM_020336.3
    Accession Version NM_020336.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-02
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform 1
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR002520.1, AB033045.3, BC047482.1, CR936800.1, BX641080.1 and BE669526.1. On Sep 21, 2013 this sequence version replaced NM_020336.2. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same isoform (1). ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC CCAAAGACTC AACTCCCAGT GGCGCCAAGA CATGAGCATA
    TCACTGGCAG CTCTAGAGCT CCTCTCTGGC CTTGCAAAGG TAAAAGTGAT GGTTGACTCA
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCACCTACA TTGTTTATCA GTGTAGTCGG
    CCAGCTCCTT TACACTCCAG GGATCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA CCCTGATATG CTTGATGAAA AGGACTGCCT TAAGGAAGTA
    CTGGAGATTG TGGAACTGGG TATCTCAGGA AGTAAGTCCA AGAACAATGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGCATTAT GCAGTTGCTC GGCGCATTTC CTTCACCTAG TGGTCCTGCC
    TCTCCTTGTA GTCTTGTGAA TGAGACCACT TTGATTAAAT ACTCCAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAA
    CAACCTCTTG GAAATGAGCA GAATGATTTT TTCCCCTCTG TCACTGTGCT GGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAG CTTTGTCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGC CCAGTTCCTA AAAATGACGT TGGATTTAAA
    TATTCTGTGA AACATCGGCC ATTTCCTGAA GAGGTGGACA AGATTCCTTT TGTGAAAGCA
    GATCTCAGCA TTCCAGATTT GCATGAAATA GTCACTGAAG AATTAGAAGA GAGACACGAA
    AAATTAAGGA GTGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA GCAACAGAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GACCCAGTTA CGGATTGCAA GCCCCCGCCT
    CCTGCCCAGG AATTCCAAAC AGCCCGCCTT TTTCTCTCAC ACTTTGGATT TTTGTCCTTA
    GAAGCACTGA AGGAACCTGC AAATAGTCGT CTACCTCCTC ACCTTATTGC ACTTGATTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCTTTT
    GACACAGTTT TTATTTTCTA TATGAAGCCA GGTCAGAAAA CGAACCAAGA GATTTTAAAG
    AATGTGGAGT CTTCCAGAAC TGTTCAGCCA CATTTCCTAG AATTTTTGCT TTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCTGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAATTGTT GTGATGATGG TGAAGGATCT CAACAAGAAG TGATTTCCTC TGAAGATATT
    GGAGCTAGCA TTTTCAATGG ACAGAAGAAG GTGCTGTATT ATGCTGATGC CCTTACAGAA
    ATTGCTTTTG TGGTTCCTTC TCCTGTGGAG TCCTTAACTG ATTCATTGGA AAGTAACATC
    TCGGACCAAG ATAGTGATTC AAATATGGAT CTTATGCCAG GAATTCTGAA ACAGCCATCC
    CTGACACTTG AGCTTTTCCC CAATCATACA GACAATCTTA ATTCCTCACA GAGGCTCAGT
    CCCAGTTCCA GAATGAGGAA GCTGCCTCAG GGTCGCCCTG TTCCTCCCCT TGGACCTGAG
    ACAAGAGTTT CTGTAGTCTG GGTGGAACGC TATGATGATA TAGAAAACTT TCCCCTCTCA
    GAGCTGATGA CAGAGATCAG TACTGGTGTG GAAACTACTG CAAATAGTAG CACTTCACTG
    AGATCTACAA CTCTTGAAAA AGAAGTTCCT GTCATCTTCA TCCACCCTTT AAACACTGGA
    TTATTCCGGA TAAAAATTCA AGGAGCCACT GGAAAATTTA ATATGGTCAT CCCTCTTGTG
    GATGGGATGA TTGTCAGCAG GCGAGCTCTT GGCTTTCTGG TGAGGCAGAC TGTAATTAAC
    ATTTGTAGAA GAAAGAGACT GGAAAGTGAC TCCTACAGTC CCCCCCATGT CCGCCGGAAA
    CAGAAAATCA CCGACATTGT CAACAAGTAC CGGAACAAGC AGCTGGAGCC AGAGTTTTAT
    ACTTCACTTT TCCAGGAGGT TGGACTCAAG AACTGCAGTT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_065069.1
    CDS295..4779
    Translation

    Target ORF information:

    RefSeq Version NM_020336.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020336.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC CCAAAGACTC AACTCCCAGT GGCGCCAAGA CATGAGCATA
    TCACTGGCAG CTCTAGAGCT CCTCTCTGGC CTTGCAAAGG TAAAAGTGAT GGTTGACTCA
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCACCTACA TTGTTTATCA GTGTAGTCGG
    CCAGCTCCTT TACACTCCAG GGATCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA CCCTGATATG CTTGATGAAA AGGACTGCCT TAAGGAAGTA
    CTGGAGATTG TGGAACTGGG TATCTCAGGA AGTAAGTCCA AGAACAATGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGCATTAT GCAGTTGCTC GGCGCATTTC CTTCACCTAG TGGTCCTGCC
    TCTCCTTGTA GTCTTGTGAA TGAGACCACT TTGATTAAAT ACTCCAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAA
    CAACCTCTTG GAAATGAGCA GAATGATTTT TTCCCCTCTG TCACTGTGCT GGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAG CTTTGTCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGC CCAGTTCCTA AAAATGACGT TGGATTTAAA
    TATTCTGTGA AACATCGGCC ATTTCCTGAA GAGGTGGACA AGATTCCTTT TGTGAAAGCA
    GATCTCAGCA TTCCAGATTT GCATGAAATA GTCACTGAAG AATTAGAAGA GAGACACGAA
    AAATTAAGGA GTGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA GCAACAGAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GACCCAGTTA CGGATTGCAA GCCCCCGCCT
    CCTGCCCAGG AATTCCAAAC AGCCCGCCTT TTTCTCTCAC ACTTTGGATT TTTGTCCTTA
    GAAGCACTGA AGGAACCTGC AAATAGTCGT CTACCTCCTC ACCTTATTGC ACTTGATTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCTTTT
    GACACAGTTT TTATTTTCTA TATGAAGCCA GGTCAGAAAA CGAACCAAGA GATTTTAAAG
    AATGTGGAGT CTTCCAGAAC TGTTCAGCCA CATTTCCTAG AATTTTTGCT TTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCTGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAATTGTT GTGATGATGG TGAAGGATCT CAACAAGAAG TGATTTCCTC TGAAGATATT
    GGAGCTAGCA TTTTCAATGG ACAGAAGAAG GTGCTGTATT ATGCTGATGC CCTTACAGAA
    ATTGCTTTTG TGGTTCCTTC TCCTGTGGAG TCCTTAACTG ATTCATTGGA AAGTAACATC
    TCGGACCAAG ATAGTGATTC AAATATGGAT CTTATGCCAG GAATTCTGAA ACAGCCATCC
    CTGACACTTG AGCTTTTCCC CAATCATACA GACAATCTTA ATTCCTCACA GAGGCTCAGT
    CCCAGTTCCA GAATGAGGAA GCTGCCTCAG GGTCGCCCTG TTCCTCCCCT TGGACCTGAG
    ACAAGAGTTT CTGTAGTCTG GGTGGAACGC TATGATGATA TAGAAAACTT TCCCCTCTCA
    GAGCTGATGA CAGAGATCAG TACTGGTGTG GAAACTACTG CAAATAGTAG CACTTCACTG
    AGATCTACAA CTCTTGAAAA AGAAGTTCCT GTCATCTTCA TCCACCCTTT AAACACTGGA
    TTATTCCGGA TAAAAATTCA AGGAGCCACT GGAAAATTTA ATATGGTCAT CCCTCTTGTG
    GATGGGATGA TTGTCAGCAG GCGAGCTCTT GGCTTTCTGG TGAGGCAGAC TGTAATTAAC
    ATTTGTAGAA GAAAGAGACT GGAAAGTGAC TCCTACAGTC CCCCCCATGT CCGCCGGAAA
    CAGAAAATCA CCGACATTGT CAACAAGTAC CGGAACAAGC AGCTGGAGCC AGAGTTTTAT
    ACTTCACTTT TCCAGGAGGT TGGACTCAAG AACTGCAGTT CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu05184
    Clone ID Related Accession (Same CDS sequence) NM_001282918.2 , NM_001282918.1
    Accession Version NM_001282918.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AL049868.20 and AL035419.12. On May 31, 2019 this sequence version replaced NM_001282918.1. Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.237564.1, AB033045.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACCC AAAGACTCAA CTCCCAGTGG CGCCAAGACA TGAGCATATC ACTGGCAGCT
    CTAGAGCTCC TCTCTGGCCT TGCAAAGGTA AAAGTGATGG TTGACTCAGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CACCTACATT GTTTATCAGT GTAGTCGGCC AGCTCCTTTA
    CACTCCAGGG ATCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAGCACC CTGATATGCT TGATGAAAAG GACTGCCTTA AGGAAGTACT GGAGATTGTG
    GAACTGGGTA TCTCAGGAAG TAAGTCCAAG AACAATGAGC AAGAGGTCAA GTACAAAGGA
    GATAAGGAGC CAAACCCTGC ATCTATGAGG GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGCATTATGC AGTTGCTCGG CGCATTTCCT TCACCTAGTG GTCCTGCCTC TCCTTGTAGT
    CTTGTGAATG AGACCACTTT GATTAAATAC TCCAGGCTGC CAACCATAAA CAAGCATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAACA ACCTCTTGGA
    AATGAGCAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTGG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAGCT TTGTCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTACCTG AACCTCGCCC AGTTCCTAAA AATGACGTTG GATTTAAATA TTCTGTGAAA
    CATCGGCCAT TTCCTGAAGA GGTGGACAAG ATTCCTTTTG TGAAAGCAGA TCTCAGCATT
    CCAGATTTGC ATGAAATAGT CACTGAAGAA TTAGAAGAGA GACACGAAAA ATTAAGGAGT
    GGCATGGCCC AGCAGATTGC TTATGAAATA CACCTTGAGC AACAGAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA CCCAGTTACG GATTGCAAGC CCCCGCCTCC TGCCCAGGAA
    TTCCAAACAG CCCGCCTTTT TCTCTCACAC TTTGGATTTT TGTCCTTAGA AGCACTGAAG
    GAACCTGCAA ATAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGATTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCTTTTGA CACAGTTTTT
    ATTTTCTATA TGAAGCCAGG TCAGAAAACG AACCAAGAGA TTTTAAAGAA TGTGGAGTCT
    TCCAGAACTG TTCAGCCACA TTTCCTAGAA TTTTTGCTTT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAGAC ACCCTGGTTG GACTGGGCAT GTTTCTACCA GTTGGTCTAT TAATTGTTGT
    GATGATGGTG AAGGATCTCA ACAAGAAGAA GTGATTTCCT CTGAAGATAT TGGAGCTAGC
    ATTTTCAATG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACAGA AATTGCTTTT
    GTGGTTCCTT CTCCTGTGGA GTCCTTAACT GATTCATTGG AAAGTAACAT CTCGGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCA GGAATTCTGA AACAGCCATC CCTGACACTT
    GAGCTTTTCC CCAATCATAC AGACAATCTT AATTCCTCAC AGAGGCTCAG TCCCAGTTCC
    AGAATGAGGA AGCTGCCTCA GGGTCGCCCT GTTCCTCCCC TTGGACCTGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAAAACT TTCCCCTCTC AGAGCTGATG
    ACAGAGATCA GTACTGGTGT GGAAACTACT GCAAATAGTA GCACTTCACT GAGATCTACA
    ACTCTTGAAA AAGAAGTTCC TGTCATCTTC ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AATATGGTCA TCCCTCTTGT GGATGGGATG
    ATTGTCAGCA GGCGAGCTCT TGGCTTTCTG GTGAGGCAGA CTGTAATTAA CATTTGTAGA
    AGAAAGAGAC TGGAAAGTGA CTCCTACAGT CCCCCCCATG TCCGCCGGAA ACAGAAAATC
    ACCGACATTG TCAACAAGTA CCGGAACAAG CAGCTGGAGC CAGAGTTTTA TACTTCACTT
    TTCCAGGAGG TTGGACTCAA GAACTGCAGT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269847.1
    CDS258..4733
    Translation

    Target ORF information:

    RefSeq Version NM_001282918.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282918.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACCC AAAGACTCAA CTCCCAGTGG CGCCAAGACA TGAGCATATC ACTGGCAGCT
    CTAGAGCTCC TCTCTGGCCT TGCAAAGGTA AAAGTGATGG TTGACTCAGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CACCTACATT GTTTATCAGT GTAGTCGGCC AGCTCCTTTA
    CACTCCAGGG ATCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAGCACC CTGATATGCT TGATGAAAAG GACTGCCTTA AGGAAGTACT GGAGATTGTG
    GAACTGGGTA TCTCAGGAAG TAAGTCCAAG AACAATGAGC AAGAGGTCAA GTACAAAGGA
    GATAAGGAGC CAAACCCTGC ATCTATGAGG GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGCATTATGC AGTTGCTCGG CGCATTTCCT TCACCTAGTG GTCCTGCCTC TCCTTGTAGT
    CTTGTGAATG AGACCACTTT GATTAAATAC TCCAGGCTGC CAACCATAAA CAAGCATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAACA ACCTCTTGGA
    AATGAGCAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTGG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAGCT TTGTCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTACCTG AACCTCGCCC AGTTCCTAAA AATGACGTTG GATTTAAATA TTCTGTGAAA
    CATCGGCCAT TTCCTGAAGA GGTGGACAAG ATTCCTTTTG TGAAAGCAGA TCTCAGCATT
    CCAGATTTGC ATGAAATAGT CACTGAAGAA TTAGAAGAGA GACACGAAAA ATTAAGGAGT
    GGCATGGCCC AGCAGATTGC TTATGAAATA CACCTTGAGC AACAGAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA CCCAGTTACG GATTGCAAGC CCCCGCCTCC TGCCCAGGAA
    TTCCAAACAG CCCGCCTTTT TCTCTCACAC TTTGGATTTT TGTCCTTAGA AGCACTGAAG
    GAACCTGCAA ATAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGATTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCTTTTGA CACAGTTTTT
    ATTTTCTATA TGAAGCCAGG TCAGAAAACG AACCAAGAGA TTTTAAAGAA TGTGGAGTCT
    TCCAGAACTG TTCAGCCACA TTTCCTAGAA TTTTTGCTTT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAGAC ACCCTGGTTG GACTGGGCAT GTTTCTACCA GTTGGTCTAT TAATTGTTGT
    GATGATGGTG AAGGATCTCA ACAAGAAGAA GTGATTTCCT CTGAAGATAT TGGAGCTAGC
    ATTTTCAATG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACAGA AATTGCTTTT
    GTGGTTCCTT CTCCTGTGGA GTCCTTAACT GATTCATTGG AAAGTAACAT CTCGGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCA GGAATTCTGA AACAGCCATC CCTGACACTT
    GAGCTTTTCC CCAATCATAC AGACAATCTT AATTCCTCAC AGAGGCTCAG TCCCAGTTCC
    AGAATGAGGA AGCTGCCTCA GGGTCGCCCT GTTCCTCCCC TTGGACCTGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAAAACT TTCCCCTCTC AGAGCTGATG
    ACAGAGATCA GTACTGGTGT GGAAACTACT GCAAATAGTA GCACTTCACT GAGATCTACA
    ACTCTTGAAA AAGAAGTTCC TGTCATCTTC ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AATATGGTCA TCCCTCTTGT GGATGGGATG
    ATTGTCAGCA GGCGAGCTCT TGGCTTTCTG GTGAGGCAGA CTGTAATTAA CATTTGTAGA
    AGAAAGAGAC TGGAAAGTGA CTCCTACAGT CCCCCCCATG TCCGCCGGAA ACAGAAAATC
    ACCGACATTG TCAACAAGTA CCGGAACAAG CAGCTGGAGC CAGAGTTTTA TACTTCACTT
    TTCCAGGAGG TTGGACTCAA GAACTGCAGT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu05184
    Clone ID Related Accession (Same CDS sequence) NM_001282918.2 , NM_001282918.1
    Accession Version NM_001282918.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4476bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-06-29
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from DR002520.1, AB033045.3, BX641080.1 and BE669526.1. On Sep 21, 2013 this sequence version replaced XM_005260466.1. Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1. It encodes isoform 2, which is shorter than isoform 1. ##Evidence-Data-START## Transcript exon combination :: AB033045.3, SRR1803611.237564.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACCC AAAGACTCAA CTCCCAGTGG CGCCAAGACA TGAGCATATC ACTGGCAGCT
    CTAGAGCTCC TCTCTGGCCT TGCAAAGGTA AAAGTGATGG TTGACTCAGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CACCTACATT GTTTATCAGT GTAGTCGGCC AGCTCCTTTA
    CACTCCAGGG ATCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAGCACC CTGATATGCT TGATGAAAAG GACTGCCTTA AGGAAGTACT GGAGATTGTG
    GAACTGGGTA TCTCAGGAAG TAAGTCCAAG AACAATGAGC AAGAGGTCAA GTACAAAGGA
    GATAAGGAGC CAAACCCTGC ATCTATGAGG GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGCATTATGC AGTTGCTCGG CGCATTTCCT TCACCTAGTG GTCCTGCCTC TCCTTGTAGT
    CTTGTGAATG AGACCACTTT GATTAAATAC TCCAGGCTGC CAACCATAAA CAAGCATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAACA ACCTCTTGGA
    AATGAGCAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTGG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAGCT TTGTCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTACCTG AACCTCGCCC AGTTCCTAAA AATGACGTTG GATTTAAATA TTCTGTGAAA
    CATCGGCCAT TTCCTGAAGA GGTGGACAAG ATTCCTTTTG TGAAAGCAGA TCTCAGCATT
    CCAGATTTGC ATGAAATAGT CACTGAAGAA TTAGAAGAGA GACACGAAAA ATTAAGGAGT
    GGCATGGCCC AGCAGATTGC TTATGAAATA CACCTTGAGC AACAGAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA CCCAGTTACG GATTGCAAGC CCCCGCCTCC TGCCCAGGAA
    TTCCAAACAG CCCGCCTTTT TCTCTCACAC TTTGGATTTT TGTCCTTAGA AGCACTGAAG
    GAACCTGCAA ATAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGATTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCTTTTGA CACAGTTTTT
    ATTTTCTATA TGAAGCCAGG TCAGAAAACG AACCAAGAGA TTTTAAAGAA TGTGGAGTCT
    TCCAGAACTG TTCAGCCACA TTTCCTAGAA TTTTTGCTTT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAGAC ACCCTGGTTG GACTGGGCAT GTTTCTACCA GTTGGTCTAT TAATTGTTGT
    GATGATGGTG AAGGATCTCA ACAAGAAGAA GTGATTTCCT CTGAAGATAT TGGAGCTAGC
    ATTTTCAATG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACAGA AATTGCTTTT
    GTGGTTCCTT CTCCTGTGGA GTCCTTAACT GATTCATTGG AAAGTAACAT CTCGGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCA GGAATTCTGA AACAGCCATC CCTGACACTT
    GAGCTTTTCC CCAATCATAC AGACAATCTT AATTCCTCAC AGAGGCTCAG TCCCAGTTCC
    AGAATGAGGA AGCTGCCTCA GGGTCGCCCT GTTCCTCCCC TTGGACCTGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAAAACT TTCCCCTCTC AGAGCTGATG
    ACAGAGATCA GTACTGGTGT GGAAACTACT GCAAATAGTA GCACTTCACT GAGATCTACA
    ACTCTTGAAA AAGAAGTTCC TGTCATCTTC ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AATATGGTCA TCCCTCTTGT GGATGGGATG
    ATTGTCAGCA GGCGAGCTCT TGGCTTTCTG GTGAGGCAGA CTGTAATTAA CATTTGTAGA
    AGAAAGAGAC TGGAAAGTGA CTCCTACAGT CCCCCCCATG TCCGCCGGAA ACAGAAAATC
    ACCGACATTG TCAACAAGTA CCGGAACAAG CAGCTGGAGC CAGAGTTTTA TACTTCACTT
    TTCCAGGAGG TTGGACTCAA GAACTGCAGT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001269847.1
    CDS295..4770
    Translation

    Target ORF information:

    RefSeq Version NM_001282918.1
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001282918.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGATTCA GCTGCTGGGC TCCTGATTCG CAGCATTCAT
    CTCGTCACCC AAAGACTCAA CTCCCAGTGG CGCCAAGACA TGAGCATATC ACTGGCAGCT
    CTAGAGCTCC TCTCTGGCCT TGCAAAGGTA AAAGTGATGG TTGACTCAGG AGACCGGAAG
    CGAGCCATCA GTTCTGTGTG CACCTACATT GTTTATCAGT GTAGTCGGCC AGCTCCTTTA
    CACTCCAGGG ATCTGCACTC CATGATAGTG GCAGCTTTTC AGTGTCTCTG TGTCTGGCTG
    ACAGAGCACC CTGATATGCT TGATGAAAAG GACTGCCTTA AGGAAGTACT GGAGATTGTG
    GAACTGGGTA TCTCAGGAAG TAAGTCCAAG AACAATGAGC AAGAGGTCAA GTACAAAGGA
    GATAAGGAGC CAAACCCTGC ATCTATGAGG GTAAAGGATG CTGCTGAAGC CACCCTAACA
    TGCATTATGC AGTTGCTCGG CGCATTTCCT TCACCTAGTG GTCCTGCCTC TCCTTGTAGT
    CTTGTGAATG AGACCACTTT GATTAAATAC TCCAGGCTGC CAACCATAAA CAAGCATAGT
    TTCCGGTACT TTGTCTTGGA TAACAGTGTC ATCCTGGCAA TGCTGGAACA ACCTCTTGGA
    AATGAGCAGA ATGATTTTTT CCCCTCTGTC ACTGTGCTGG TCCGGGGAAT GTCTGGAAGA
    CTTGCTTGGG CACAACAGCT TTGTCTTTTA CCCAGAGGAG CAAAAGCAAA TCAGAAGCTT
    TTTGTACCTG AACCTCGCCC AGTTCCTAAA AATGACGTTG GATTTAAATA TTCTGTGAAA
    CATCGGCCAT TTCCTGAAGA GGTGGACAAG ATTCCTTTTG TGAAAGCAGA TCTCAGCATT
    CCAGATTTGC ATGAAATAGT CACTGAAGAA TTAGAAGAGA GACACGAAAA ATTAAGGAGT
    GGCATGGCCC AGCAGATTGC TTATGAAATA CACCTTGAGC AACAGAGTGA GGAGGAATTG
    CAGAAGAGAA GTTTTCCTGA CCCAGTTACG GATTGCAAGC CCCCGCCTCC TGCCCAGGAA
    TTCCAAACAG CCCGCCTTTT TCTCTCACAC TTTGGATTTT TGTCCTTAGA AGCACTGAAG
    GAACCTGCAA ATAGTCGTCT ACCTCCTCAC CTTATTGCAC TTGATTCCAC GATACCTGGA
    TTTTTTGATG ACATTGGGTA TCTGGATCTC TTGCCATGTC GTCCTTTTGA CACAGTTTTT
    ATTTTCTATA TGAAGCCAGG TCAGAAAACG AACCAAGAGA TTTTAAAGAA TGTGGAGTCT
    TCCAGAACTG TTCAGCCACA TTTCCTAGAA TTTTTGCTTT CCCTTGGCTG GTCAGTAGAT
    GTGGGCAGAC ACCCTGGTTG GACTGGGCAT GTTTCTACCA GTTGGTCTAT TAATTGTTGT
    GATGATGGTG AAGGATCTCA ACAAGAAGAA GTGATTTCCT CTGAAGATAT TGGAGCTAGC
    ATTTTCAATG GACAGAAGAA GGTGCTGTAT TATGCTGATG CCCTTACAGA AATTGCTTTT
    GTGGTTCCTT CTCCTGTGGA GTCCTTAACT GATTCATTGG AAAGTAACAT CTCGGACCAA
    GATAGTGATT CAAATATGGA TCTTATGCCA GGAATTCTGA AACAGCCATC CCTGACACTT
    GAGCTTTTCC CCAATCATAC AGACAATCTT AATTCCTCAC AGAGGCTCAG TCCCAGTTCC
    AGAATGAGGA AGCTGCCTCA GGGTCGCCCT GTTCCTCCCC TTGGACCTGA GACAAGAGTT
    TCTGTAGTCT GGGTGGAACG CTATGATGAT ATAGAAAACT TTCCCCTCTC AGAGCTGATG
    ACAGAGATCA GTACTGGTGT GGAAACTACT GCAAATAGTA GCACTTCACT GAGATCTACA
    ACTCTTGAAA AAGAAGTTCC TGTCATCTTC ATCCACCCTT TAAACACTGG ATTATTCCGG
    ATAAAAATTC AAGGAGCCAC TGGAAAATTT AATATGGTCA TCCCTCTTGT GGATGGGATG
    ATTGTCAGCA GGCGAGCTCT TGGCTTTCTG GTGAGGCAGA CTGTAATTAA CATTTGTAGA
    AGAAAGAGAC TGGAAAGTGA CTCCTACAGT CCCCCCCATG TCCGCCGGAA ACAGAAAATC
    ACCGACATTG TCAACAAGTA CCGGAACAAG CAGCTGGAGC CAGAGTTTTA TACTTCACTT
    TTCCAGGAGG TTGGACTCAA GAACTGCAGT TCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53051
    Clone ID Related Accession (Same CDS sequence) XM_005260462.3 , XM_005260462.2
    Accession Version XM_005260462.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005260462.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GCTCTTAATA CAGATTCAGC TGCTGGGCTC CTGATTCGCA GCATTCATCT CGTCACCCAA
    AGACTCAACT CCCAGTGGCG CCAAGACATG AGCATATCAC TGGCAGCTCT AGAGCTCCTC
    TCTGGCCTTG CAAAGGTAAA AGTGATGGTT GACTCAGGAG ACCGGAAGCG AGCCATCAGT
    TCTGTGTGCA CCTACATTGT TTATCAGTGT AGTCGGCCAG CTCCTTTACA CTCCAGGGAT
    CTGCACTCCA TGATAGTGGC AGCTTTTCAG TGTCTCTGTG TCTGGCTGAC AGAGCACCCT
    GATATGCTTG ATGAAAAGGA CTGCCTTAAG GAAGTACTGG AGATTGTGGA ACTGGGTATC
    TCAGGAAGTA AGTCCAAGAA CAATGAGCAA GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA
    AACCCTGCAT CTATGAGGGT AAAGGATGCT GCTGAAGCCA CCCTAACATG CATTATGCAG
    TTGCTCGGCG CATTTCCTTC ACCTAGTGGT CCTGCCTCTC CTTGTAGTCT TGTGAATGAG
    ACCACTTTGA TTAAATACTC CAGGCTGCCA ACCATAAACA AGCATAGTTT CCGGTACTTT
    GTCTTGGATA ACAGTGTCAT CCTGGCAATG CTGGAACAAC CTCTTGGAAA TGAGCAGAAT
    GATTTTTTCC CCTCTGTCAC TGTGCTGGTC CGGGGAATGT CTGGAAGACT TGCTTGGGCA
    CAACAGCTTT GTCTTTTACC CAGAGGAGCA AAAGCAAATC AGAAGCTTTT TGTACCTGAA
    CCTCGCCCAG TTCCTAAAAA TGACGTTGGA TTTAAATATT CTGTGAAACA TCGGCCATTT
    CCTGAAGAGG TGGACAAGAT TCCTTTTGTG AAAGCAGATC TCAGCATTCC AGATTTGCAT
    GAAATAGTCA CTGAAGAATT AGAAGAGAGA CACGAAAAAT TAAGGAGTGG CATGGCCCAG
    CAGATTGCTT ATGAAATACA CCTTGAGCAA CAGAGTGAGG AGGAATTGCA GAAGAGAAGT
    TTTCCTGACC CAGTTACGGA TTGCAAGCCC CCGCCTCCTG CCCAGGAATT CCAAACAGCC
    CGCCTTTTTC TCTCACACTT TGGATTTTTG TCCTTAGAAG CACTGAAGGA ACCTGCAAAT
    AGTCGTCTAC CTCCTCACCT TATTGCACTT GATTCCACGA TACCTGGATT TTTTGATGAC
    ATTGGGTATC TGGATCTCTT GCCATGTCGT CCTTTTGACA CAGTTTTTAT TTTCTATATG
    AAGCCAGGTC AGAAAACGAA CCAAGAGATT TTAAAGAATG TGGAGTCTTC CAGAACTGTT
    CAGCCACATT TCCTAGAATT TTTGCTTTCC CTTGGCTGGT CAGTAGATGT GGGCAGACAC
    CCTGGTTGGA CTGGGCATGT TTCTACCAGT TGGTCTATTA ATTGTTGTGA TGATGGTGAA
    GGATCTCAAC AAGAAGAAGT GATTTCCTCT GAAGATATTG GAGCTAGCAT TTTCAATGGA
    CAGAAGAAGG TGCTGTATTA TGCTGATGCC CTTACAGAAA TTGCTTTTGT GGTTCCTTCT
    CCTGTGGAGT CCTTAACTGA TTCATTGGAA AGTAACATCT CGGACCAAGA TAGTGATTCA
    AATATGGATC TTATGCCAGG AATTCTGAAA CAGCCATCCC TGACACTTGA GCTTTTCCCC
    AATCATACAG ACAATCTTAA TTCCTCACAG AGGCTCAGTC CCAGTTCCAG AATGAGGAAG
    CTGCCTCAGG GTCGCCCTGT TCCTCCCCTT GGACCTGAGA CAAGAGTTTC TGTAGTCTGG
    GTGGAACGCT ATGATGATAT AGAAAACTTT CCCCTCTCAG AGCTGATGAC AGAGATCAGT
    ACTGGTGTGG AAACTACTGC AAATAGTAGC ACTTCACTGA GATCTACAAC TCTTGAAAAA
    GAAGTTCCTG TCATCTTCAT CCACCCTTTA AACACTGGAT TATTCCGGAT AAAAATTCAA
    GGAGCCACTG GAAAATTTAA TATGGTCATC CCTCTTGTGG ATGGGATGAT TGTCAGCAGG
    CGAGCTCTTG GCTTTCTGGT GAGGCAGACT GTAATTAACA TTTGTAGAAG AAAGAGACTG
    GAAAGTGACT CCTACAGTCC CCCCCATGTC CGCCGGAAAC AGAAAATCAC CGACATTGTC
    AACAAGTACC GGAACAAGCA GCTGGAGCCA GAGTTTTATA CTTCACTTTT CCAGGAGGTT
    GGACTCAAGA ACTGCAGTTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005260519.1
    CDS281..4804
    Translation

    Target ORF information:

    RefSeq Version XM_005260462.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005260462.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GCTCTTAATA CAGATTCAGC TGCTGGGCTC CTGATTCGCA GCATTCATCT CGTCACCCAA
    AGACTCAACT CCCAGTGGCG CCAAGACATG AGCATATCAC TGGCAGCTCT AGAGCTCCTC
    TCTGGCCTTG CAAAGGTAAA AGTGATGGTT GACTCAGGAG ACCGGAAGCG AGCCATCAGT
    TCTGTGTGCA CCTACATTGT TTATCAGTGT AGTCGGCCAG CTCCTTTACA CTCCAGGGAT
    CTGCACTCCA TGATAGTGGC AGCTTTTCAG TGTCTCTGTG TCTGGCTGAC AGAGCACCCT
    GATATGCTTG ATGAAAAGGA CTGCCTTAAG GAAGTACTGG AGATTGTGGA ACTGGGTATC
    TCAGGAAGTA AGTCCAAGAA CAATGAGCAA GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA
    AACCCTGCAT CTATGAGGGT AAAGGATGCT GCTGAAGCCA CCCTAACATG CATTATGCAG
    TTGCTCGGCG CATTTCCTTC ACCTAGTGGT CCTGCCTCTC CTTGTAGTCT TGTGAATGAG
    ACCACTTTGA TTAAATACTC CAGGCTGCCA ACCATAAACA AGCATAGTTT CCGGTACTTT
    GTCTTGGATA ACAGTGTCAT CCTGGCAATG CTGGAACAAC CTCTTGGAAA TGAGCAGAAT
    GATTTTTTCC CCTCTGTCAC TGTGCTGGTC CGGGGAATGT CTGGAAGACT TGCTTGGGCA
    CAACAGCTTT GTCTTTTACC CAGAGGAGCA AAAGCAAATC AGAAGCTTTT TGTACCTGAA
    CCTCGCCCAG TTCCTAAAAA TGACGTTGGA TTTAAATATT CTGTGAAACA TCGGCCATTT
    CCTGAAGAGG TGGACAAGAT TCCTTTTGTG AAAGCAGATC TCAGCATTCC AGATTTGCAT
    GAAATAGTCA CTGAAGAATT AGAAGAGAGA CACGAAAAAT TAAGGAGTGG CATGGCCCAG
    CAGATTGCTT ATGAAATACA CCTTGAGCAA CAGAGTGAGG AGGAATTGCA GAAGAGAAGT
    TTTCCTGACC CAGTTACGGA TTGCAAGCCC CCGCCTCCTG CCCAGGAATT CCAAACAGCC
    CGCCTTTTTC TCTCACACTT TGGATTTTTG TCCTTAGAAG CACTGAAGGA ACCTGCAAAT
    AGTCGTCTAC CTCCTCACCT TATTGCACTT GATTCCACGA TACCTGGATT TTTTGATGAC
    ATTGGGTATC TGGATCTCTT GCCATGTCGT CCTTTTGACA CAGTTTTTAT TTTCTATATG
    AAGCCAGGTC AGAAAACGAA CCAAGAGATT TTAAAGAATG TGGAGTCTTC CAGAACTGTT
    CAGCCACATT TCCTAGAATT TTTGCTTTCC CTTGGCTGGT CAGTAGATGT GGGCAGACAC
    CCTGGTTGGA CTGGGCATGT TTCTACCAGT TGGTCTATTA ATTGTTGTGA TGATGGTGAA
    GGATCTCAAC AAGAAGAAGT GATTTCCTCT GAAGATATTG GAGCTAGCAT TTTCAATGGA
    CAGAAGAAGG TGCTGTATTA TGCTGATGCC CTTACAGAAA TTGCTTTTGT GGTTCCTTCT
    CCTGTGGAGT CCTTAACTGA TTCATTGGAA AGTAACATCT CGGACCAAGA TAGTGATTCA
    AATATGGATC TTATGCCAGG AATTCTGAAA CAGCCATCCC TGACACTTGA GCTTTTCCCC
    AATCATACAG ACAATCTTAA TTCCTCACAG AGGCTCAGTC CCAGTTCCAG AATGAGGAAG
    CTGCCTCAGG GTCGCCCTGT TCCTCCCCTT GGACCTGAGA CAAGAGTTTC TGTAGTCTGG
    GTGGAACGCT ATGATGATAT AGAAAACTTT CCCCTCTCAG AGCTGATGAC AGAGATCAGT
    ACTGGTGTGG AAACTACTGC AAATAGTAGC ACTTCACTGA GATCTACAAC TCTTGAAAAA
    GAAGTTCCTG TCATCTTCAT CCACCCTTTA AACACTGGAT TATTCCGGAT AAAAATTCAA
    GGAGCCACTG GAAAATTTAA TATGGTCATC CCTCTTGTGG ATGGGATGAT TGTCAGCAGG
    CGAGCTCTTG GCTTTCTGGT GAGGCAGACT GTAATTAACA TTTGTAGAAG AAAGAGACTG
    GAAAGTGACT CCTACAGTCC CCCCCATGTC CGCCGGAAAC AGAAAATCAC CGACATTGTC
    AACAAGTACC GGAACAAGCA GCTGGAGCCA GAGTTTTATA CTTCACTTTT CCAGGAGGTT
    GGACTCAAGA ACTGCAGTTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53051
    Clone ID Related Accession (Same CDS sequence) XM_005260462.3 , XM_005260462.2
    Accession Version XM_005260462.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260462.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GCTCTTAATA CAGATTCAGC TGCTGGGCTC CTGATTCGCA GCATTCATCT CGTCACCCAA
    AGACTCAACT CCCAGTGGCG CCAAGACATG AGCATATCAC TGGCAGCTCT AGAGCTCCTC
    TCTGGCCTTG CAAAGGTAAA AGTGATGGTT GACTCAGGAG ACCGGAAGCG AGCCATCAGT
    TCTGTGTGCA CCTACATTGT TTATCAGTGT AGTCGGCCAG CTCCTTTACA CTCCAGGGAT
    CTGCACTCCA TGATAGTGGC AGCTTTTCAG TGTCTCTGTG TCTGGCTGAC AGAGCACCCT
    GATATGCTTG ATGAAAAGGA CTGCCTTAAG GAAGTACTGG AGATTGTGGA ACTGGGTATC
    TCAGGAAGTA AGTCCAAGAA CAATGAGCAA GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA
    AACCCTGCAT CTATGAGGGT AAAGGATGCT GCTGAAGCCA CCCTAACATG CATTATGCAG
    TTGCTCGGCG CATTTCCTTC ACCTAGTGGT CCTGCCTCTC CTTGTAGTCT TGTGAATGAG
    ACCACTTTGA TTAAATACTC CAGGCTGCCA ACCATAAACA AGCATAGTTT CCGGTACTTT
    GTCTTGGATA ACAGTGTCAT CCTGGCAATG CTGGAACAAC CTCTTGGAAA TGAGCAGAAT
    GATTTTTTCC CCTCTGTCAC TGTGCTGGTC CGGGGAATGT CTGGAAGACT TGCTTGGGCA
    CAACAGCTTT GTCTTTTACC CAGAGGAGCA AAAGCAAATC AGAAGCTTTT TGTACCTGAA
    CCTCGCCCAG TTCCTAAAAA TGACGTTGGA TTTAAATATT CTGTGAAACA TCGGCCATTT
    CCTGAAGAGG TGGACAAGAT TCCTTTTGTG AAAGCAGATC TCAGCATTCC AGATTTGCAT
    GAAATAGTCA CTGAAGAATT AGAAGAGAGA CACGAAAAAT TAAGGAGTGG CATGGCCCAG
    CAGATTGCTT ATGAAATACA CCTTGAGCAA CAGAGTGAGG AGGAATTGCA GAAGAGAAGT
    TTTCCTGACC CAGTTACGGA TTGCAAGCCC CCGCCTCCTG CCCAGGAATT CCAAACAGCC
    CGCCTTTTTC TCTCACACTT TGGATTTTTG TCCTTAGAAG CACTGAAGGA ACCTGCAAAT
    AGTCGTCTAC CTCCTCACCT TATTGCACTT GATTCCACGA TACCTGGATT TTTTGATGAC
    ATTGGGTATC TGGATCTCTT GCCATGTCGT CCTTTTGACA CAGTTTTTAT TTTCTATATG
    AAGCCAGGTC AGAAAACGAA CCAAGAGATT TTAAAGAATG TGGAGTCTTC CAGAACTGTT
    CAGCCACATT TCCTAGAATT TTTGCTTTCC CTTGGCTGGT CAGTAGATGT GGGCAGACAC
    CCTGGTTGGA CTGGGCATGT TTCTACCAGT TGGTCTATTA ATTGTTGTGA TGATGGTGAA
    GGATCTCAAC AAGAAGAAGT GATTTCCTCT GAAGATATTG GAGCTAGCAT TTTCAATGGA
    CAGAAGAAGG TGCTGTATTA TGCTGATGCC CTTACAGAAA TTGCTTTTGT GGTTCCTTCT
    CCTGTGGAGT CCTTAACTGA TTCATTGGAA AGTAACATCT CGGACCAAGA TAGTGATTCA
    AATATGGATC TTATGCCAGG AATTCTGAAA CAGCCATCCC TGACACTTGA GCTTTTCCCC
    AATCATACAG ACAATCTTAA TTCCTCACAG AGGCTCAGTC CCAGTTCCAG AATGAGGAAG
    CTGCCTCAGG GTCGCCCTGT TCCTCCCCTT GGACCTGAGA CAAGAGTTTC TGTAGTCTGG
    GTGGAACGCT ATGATGATAT AGAAAACTTT CCCCTCTCAG AGCTGATGAC AGAGATCAGT
    ACTGGTGTGG AAACTACTGC AAATAGTAGC ACTTCACTGA GATCTACAAC TCTTGAAAAA
    GAAGTTCCTG TCATCTTCAT CCACCCTTTA AACACTGGAT TATTCCGGAT AAAAATTCAA
    GGAGCCACTG GAAAATTTAA TATGGTCATC CCTCTTGTGG ATGGGATGAT TGTCAGCAGG
    CGAGCTCTTG GCTTTCTGGT GAGGCAGACT GTAATTAACA TTTGTAGAAG AAAGAGACTG
    GAAAGTGACT CCTACAGTCC CCCCCATGTC CGCCGGAAAC AGAAAATCAC CGACATTGTC
    AACAAGTACC GGAACAAGCA GCTGGAGCCA GAGTTTTATA CTTCACTTTT CCAGGAGGTT
    GGACTCAAGA ACTGCAGTTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005260519.1
    CDS253..4776
    Translation

    Target ORF information:

    RefSeq Version XM_005260462.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005260462.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GCTCTTAATA CAGATTCAGC TGCTGGGCTC CTGATTCGCA GCATTCATCT CGTCACCCAA
    AGACTCAACT CCCAGTGGCG CCAAGACATG AGCATATCAC TGGCAGCTCT AGAGCTCCTC
    TCTGGCCTTG CAAAGGTAAA AGTGATGGTT GACTCAGGAG ACCGGAAGCG AGCCATCAGT
    TCTGTGTGCA CCTACATTGT TTATCAGTGT AGTCGGCCAG CTCCTTTACA CTCCAGGGAT
    CTGCACTCCA TGATAGTGGC AGCTTTTCAG TGTCTCTGTG TCTGGCTGAC AGAGCACCCT
    GATATGCTTG ATGAAAAGGA CTGCCTTAAG GAAGTACTGG AGATTGTGGA ACTGGGTATC
    TCAGGAAGTA AGTCCAAGAA CAATGAGCAA GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA
    AACCCTGCAT CTATGAGGGT AAAGGATGCT GCTGAAGCCA CCCTAACATG CATTATGCAG
    TTGCTCGGCG CATTTCCTTC ACCTAGTGGT CCTGCCTCTC CTTGTAGTCT TGTGAATGAG
    ACCACTTTGA TTAAATACTC CAGGCTGCCA ACCATAAACA AGCATAGTTT CCGGTACTTT
    GTCTTGGATA ACAGTGTCAT CCTGGCAATG CTGGAACAAC CTCTTGGAAA TGAGCAGAAT
    GATTTTTTCC CCTCTGTCAC TGTGCTGGTC CGGGGAATGT CTGGAAGACT TGCTTGGGCA
    CAACAGCTTT GTCTTTTACC CAGAGGAGCA AAAGCAAATC AGAAGCTTTT TGTACCTGAA
    CCTCGCCCAG TTCCTAAAAA TGACGTTGGA TTTAAATATT CTGTGAAACA TCGGCCATTT
    CCTGAAGAGG TGGACAAGAT TCCTTTTGTG AAAGCAGATC TCAGCATTCC AGATTTGCAT
    GAAATAGTCA CTGAAGAATT AGAAGAGAGA CACGAAAAAT TAAGGAGTGG CATGGCCCAG
    CAGATTGCTT ATGAAATACA CCTTGAGCAA CAGAGTGAGG AGGAATTGCA GAAGAGAAGT
    TTTCCTGACC CAGTTACGGA TTGCAAGCCC CCGCCTCCTG CCCAGGAATT CCAAACAGCC
    CGCCTTTTTC TCTCACACTT TGGATTTTTG TCCTTAGAAG CACTGAAGGA ACCTGCAAAT
    AGTCGTCTAC CTCCTCACCT TATTGCACTT GATTCCACGA TACCTGGATT TTTTGATGAC
    ATTGGGTATC TGGATCTCTT GCCATGTCGT CCTTTTGACA CAGTTTTTAT TTTCTATATG
    AAGCCAGGTC AGAAAACGAA CCAAGAGATT TTAAAGAATG TGGAGTCTTC CAGAACTGTT
    CAGCCACATT TCCTAGAATT TTTGCTTTCC CTTGGCTGGT CAGTAGATGT GGGCAGACAC
    CCTGGTTGGA CTGGGCATGT TTCTACCAGT TGGTCTATTA ATTGTTGTGA TGATGGTGAA
    GGATCTCAAC AAGAAGAAGT GATTTCCTCT GAAGATATTG GAGCTAGCAT TTTCAATGGA
    CAGAAGAAGG TGCTGTATTA TGCTGATGCC CTTACAGAAA TTGCTTTTGT GGTTCCTTCT
    CCTGTGGAGT CCTTAACTGA TTCATTGGAA AGTAACATCT CGGACCAAGA TAGTGATTCA
    AATATGGATC TTATGCCAGG AATTCTGAAA CAGCCATCCC TGACACTTGA GCTTTTCCCC
    AATCATACAG ACAATCTTAA TTCCTCACAG AGGCTCAGTC CCAGTTCCAG AATGAGGAAG
    CTGCCTCAGG GTCGCCCTGT TCCTCCCCTT GGACCTGAGA CAAGAGTTTC TGTAGTCTGG
    GTGGAACGCT ATGATGATAT AGAAAACTTT CCCCTCTCAG AGCTGATGAC AGAGATCAGT
    ACTGGTGTGG AAACTACTGC AAATAGTAGC ACTTCACTGA GATCTACAAC TCTTGAAAAA
    GAAGTTCCTG TCATCTTCAT CCACCCTTTA AACACTGGAT TATTCCGGAT AAAAATTCAA
    GGAGCCACTG GAAAATTTAA TATGGTCATC CCTCTTGTGG ATGGGATGAT TGTCAGCAGG
    CGAGCTCTTG GCTTTCTGGT GAGGCAGACT GTAATTAACA TTTGTAGAAG AAAGAGACTG
    GAAAGTGACT CCTACAGTCC CCCCCATGTC CGCCGGAAAC AGAAAATCAC CGACATTGTC
    AACAAGTACC GGAACAAGCA GCTGGAGCCA GAGTTTTATA CTTCACTTTT CCAGGAGGTT
    GGACTCAAGA ACTGCAGTTC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53052
    Clone ID Related Accession (Same CDS sequence) XM_005260463.3 , XM_005260463.2
    Accession Version XM_005260463.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005260463.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GCTCTTAATA CAGATTCAGC TGCTGGGCTC CTGATTCGCA GCATTCATCT CGTCACCCAA
    AGACTCAACT CCCAGTGGCG CCAAGACATG AGCATATCAC TGGCAGCTCT AGAGCTCCTC
    TCTGGCCTTG CAAAGGTAAA AGTGATGGTT GACTCAGGAG ACCGGAAGCG AGCCATCAGT
    TCTGTGTGCA CCTACATTGT TTATCAGTGT AGTCGGCCAG CTCCTTTACA CTCCAGGGAT
    CTGCACTCCA TGATAGTGGC AGCTTTTCAG TGTCTCTGTG TCTGGCTGAC AGAGCACCCT
    GATATGCTTG ATGAAAAGGA CTGCCTTAAG GAAGTACTGG AGATTGTGGA ACTGGGTATC
    TCAGGAAGTA AGTCCAAGAA CAATGAGCAA GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA
    AACCCTGCAT CTATGAGGGT AAAGGATGCT GCTGAAGCCA CCCTAACATG CATTATGCAG
    TTGCTCGGCG CATTTCCTTC ACCTAGTGGT CCTGCCTCTC CTTGTAGTCT TGTGAATGAG
    ACCACTTTGA TTAAATACTC CAGGCTGCCA ACCATAAACA AGCATAGTTT CCGGTACTTT
    GTCTTGGATA ACAGTGTCAT CCTGGCAATG CTGGAACAAC CTCTTGGAAA TGAGCAGAAT
    GATTTTTTCC CCTCTGTCAC TGTGCTGGTC CGGGGAATGT CTGGAAGACT TGCTTGGGCA
    CAACAGCTTT GTCTTTTACC CAGAGGAGCA AAAGCAAATC AGAAGCTTTT TGTACCTGAA
    CCTCGCCCAG TTCCTAAAAA TGACGTTGGA TTTAAATATT CTGTGAAACA TCGGCCATTT
    CCTGAAGAGG TGGACAAGAT TCCTTTTGTG AAAGCAGATC TCAGCATTCC AGATTTGCAT
    GAAATAGTCA CTGAAGAATT AGAAGAGAGA CACGAAAAAT TAAGGAGTGG CATGGCCCAG
    CAGATTGCTT ATGAAATACA CCTTGAGCAA CAGAGTGAGG AGGAATTGCA GAAGAGAAGT
    TTTCCTGACC CAGTTACGGA TTGCAAGCCC CCGCCTCCTG CCCAGGAATT CCAAACAGCC
    CGCCTTTTTC TCTCACACTT TGGATTTTTG TCCTTAGAAG CACTGAAGGA ACCTGCAAAT
    AGTCGTCTAC CTCCTCACCT TATTGCACTT GATTCCACGA TACCTGGATT TTTTGATGAC
    ATTGGGTATC TGGATCTCTT GCCATGTCGT CCTTTTGACA CAGTTTTTAT TTTCTATATG
    AAGCCAGGTC AGAAAACGAA CCAAGAGATT TTAAAGAATG TGGAGTCTTC CAGAACTGTT
    CAGCCACATT TCCTAGAATT TTTGCTTTCC CTTGGCTGGT CAGTAGATGT GGGCAGACAC
    CCTGGTTGGA CTGGGCATGT TTCTACCAGT TGGTCTATTA ATTGTTGTGA TGATGGTGAA
    GGATCTCAAC AAGAAGTGAT TTCCTCTGAA GATATTGGAG CTAGCATTTT CAATGGACAG
    AAGAAGGTGC TGTATTATGC TGATGCCCTT ACAGAAATTG CTTTTGTGGT TCCTTCTCCT
    GTGGAGTCCT TAACTGATTC ATTGGAAAGT AACATCTCGG ACCAAGATAG TGATTCAAAT
    ATGGATCTTA TGCCAGGAAT TCTGAAACAG CCATCCCTGA CACTTGAGCT TTTCCCCAAT
    CATACAGACA ATCTTAATTC CTCACAGAGG CTCAGTCCCA GTTCCAGAAT GAGGAAGCTG
    CCTCAGGGTC GCCCTGTTCC TCCCCTTGGA CCTGAGACAA GAGTTTCTGT AGTCTGGGTG
    GAACGCTATG ATGATATAGA AAACTTTCCC CTCTCAGAGC TGATGACAGA GATCAGTACT
    GGTGTGGAAA CTACTGCAAA TAGTAGCACT TCACTGAGAT CTACAACTCT TGAAAAAGAA
    GTTCCTGTCA TCTTCATCCA CCCTTTAAAC ACTGGATTAT TCCGGATAAA AATTCAAGGA
    GCCACTGGAA AATTTAATAT GGTCATCCCT CTTGTGGATG GGATGATTGT CAGCAGGCGA
    GCTCTTGGCT TTCTGGTGAG GCAGACTGTA ATTAACATTT GTAGAAGAAA GAGACTGGAA
    AGTGACTCCT ACAGTCCCCC CCATGTCCGC CGGAAACAGA AAATCACCGA CATTGTCAAC
    AAGTACCGGA ACAAGCAGCT GGAGCCAGAG TTTTATACTT CACTTTTCCA GGAGGTTGGA
    CTCAAGAACT GCAGTTCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005260520.1
    CDS281..4801
    Translation

    Target ORF information:

    RefSeq Version XM_005260463.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005260463.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GCTCTTAATA CAGATTCAGC TGCTGGGCTC CTGATTCGCA GCATTCATCT CGTCACCCAA
    AGACTCAACT CCCAGTGGCG CCAAGACATG AGCATATCAC TGGCAGCTCT AGAGCTCCTC
    TCTGGCCTTG CAAAGGTAAA AGTGATGGTT GACTCAGGAG ACCGGAAGCG AGCCATCAGT
    TCTGTGTGCA CCTACATTGT TTATCAGTGT AGTCGGCCAG CTCCTTTACA CTCCAGGGAT
    CTGCACTCCA TGATAGTGGC AGCTTTTCAG TGTCTCTGTG TCTGGCTGAC AGAGCACCCT
    GATATGCTTG ATGAAAAGGA CTGCCTTAAG GAAGTACTGG AGATTGTGGA ACTGGGTATC
    TCAGGAAGTA AGTCCAAGAA CAATGAGCAA GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA
    AACCCTGCAT CTATGAGGGT AAAGGATGCT GCTGAAGCCA CCCTAACATG CATTATGCAG
    TTGCTCGGCG CATTTCCTTC ACCTAGTGGT CCTGCCTCTC CTTGTAGTCT TGTGAATGAG
    ACCACTTTGA TTAAATACTC CAGGCTGCCA ACCATAAACA AGCATAGTTT CCGGTACTTT
    GTCTTGGATA ACAGTGTCAT CCTGGCAATG CTGGAACAAC CTCTTGGAAA TGAGCAGAAT
    GATTTTTTCC CCTCTGTCAC TGTGCTGGTC CGGGGAATGT CTGGAAGACT TGCTTGGGCA
    CAACAGCTTT GTCTTTTACC CAGAGGAGCA AAAGCAAATC AGAAGCTTTT TGTACCTGAA
    CCTCGCCCAG TTCCTAAAAA TGACGTTGGA TTTAAATATT CTGTGAAACA TCGGCCATTT
    CCTGAAGAGG TGGACAAGAT TCCTTTTGTG AAAGCAGATC TCAGCATTCC AGATTTGCAT
    GAAATAGTCA CTGAAGAATT AGAAGAGAGA CACGAAAAAT TAAGGAGTGG CATGGCCCAG
    CAGATTGCTT ATGAAATACA CCTTGAGCAA CAGAGTGAGG AGGAATTGCA GAAGAGAAGT
    TTTCCTGACC CAGTTACGGA TTGCAAGCCC CCGCCTCCTG CCCAGGAATT CCAAACAGCC
    CGCCTTTTTC TCTCACACTT TGGATTTTTG TCCTTAGAAG CACTGAAGGA ACCTGCAAAT
    AGTCGTCTAC CTCCTCACCT TATTGCACTT GATTCCACGA TACCTGGATT TTTTGATGAC
    ATTGGGTATC TGGATCTCTT GCCATGTCGT CCTTTTGACA CAGTTTTTAT TTTCTATATG
    AAGCCAGGTC AGAAAACGAA CCAAGAGATT TTAAAGAATG TGGAGTCTTC CAGAACTGTT
    CAGCCACATT TCCTAGAATT TTTGCTTTCC CTTGGCTGGT CAGTAGATGT GGGCAGACAC
    CCTGGTTGGA CTGGGCATGT TTCTACCAGT TGGTCTATTA ATTGTTGTGA TGATGGTGAA
    GGATCTCAAC AAGAAGTGAT TTCCTCTGAA GATATTGGAG CTAGCATTTT CAATGGACAG
    AAGAAGGTGC TGTATTATGC TGATGCCCTT ACAGAAATTG CTTTTGTGGT TCCTTCTCCT
    GTGGAGTCCT TAACTGATTC ATTGGAAAGT AACATCTCGG ACCAAGATAG TGATTCAAAT
    ATGGATCTTA TGCCAGGAAT TCTGAAACAG CCATCCCTGA CACTTGAGCT TTTCCCCAAT
    CATACAGACA ATCTTAATTC CTCACAGAGG CTCAGTCCCA GTTCCAGAAT GAGGAAGCTG
    CCTCAGGGTC GCCCTGTTCC TCCCCTTGGA CCTGAGACAA GAGTTTCTGT AGTCTGGGTG
    GAACGCTATG ATGATATAGA AAACTTTCCC CTCTCAGAGC TGATGACAGA GATCAGTACT
    GGTGTGGAAA CTACTGCAAA TAGTAGCACT TCACTGAGAT CTACAACTCT TGAAAAAGAA
    GTTCCTGTCA TCTTCATCCA CCCTTTAAAC ACTGGATTAT TCCGGATAAA AATTCAAGGA
    GCCACTGGAA AATTTAATAT GGTCATCCCT CTTGTGGATG GGATGATTGT CAGCAGGCGA
    GCTCTTGGCT TTCTGGTGAG GCAGACTGTA ATTAACATTT GTAGAAGAAA GAGACTGGAA
    AGTGACTCCT ACAGTCCCCC CCATGTCCGC CGGAAACAGA AAATCACCGA CATTGTCAAC
    AAGTACCGGA ACAAGCAGCT GGAGCCAGAG TTTTATACTT CACTTTTCCA GGAGGTTGGA
    CTCAAGAACT GCAGTTCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53052
    Clone ID Related Accession (Same CDS sequence) XM_005260463.3 , XM_005260463.2
    Accession Version XM_005260463.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4521bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260463.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GCTCTTAATA CAGATTCAGC TGCTGGGCTC CTGATTCGCA GCATTCATCT CGTCACCCAA
    AGACTCAACT CCCAGTGGCG CCAAGACATG AGCATATCAC TGGCAGCTCT AGAGCTCCTC
    TCTGGCCTTG CAAAGGTAAA AGTGATGGTT GACTCAGGAG ACCGGAAGCG AGCCATCAGT
    TCTGTGTGCA CCTACATTGT TTATCAGTGT AGTCGGCCAG CTCCTTTACA CTCCAGGGAT
    CTGCACTCCA TGATAGTGGC AGCTTTTCAG TGTCTCTGTG TCTGGCTGAC AGAGCACCCT
    GATATGCTTG ATGAAAAGGA CTGCCTTAAG GAAGTACTGG AGATTGTGGA ACTGGGTATC
    TCAGGAAGTA AGTCCAAGAA CAATGAGCAA GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA
    AACCCTGCAT CTATGAGGGT AAAGGATGCT GCTGAAGCCA CCCTAACATG CATTATGCAG
    TTGCTCGGCG CATTTCCTTC ACCTAGTGGT CCTGCCTCTC CTTGTAGTCT TGTGAATGAG
    ACCACTTTGA TTAAATACTC CAGGCTGCCA ACCATAAACA AGCATAGTTT CCGGTACTTT
    GTCTTGGATA ACAGTGTCAT CCTGGCAATG CTGGAACAAC CTCTTGGAAA TGAGCAGAAT
    GATTTTTTCC CCTCTGTCAC TGTGCTGGTC CGGGGAATGT CTGGAAGACT TGCTTGGGCA
    CAACAGCTTT GTCTTTTACC CAGAGGAGCA AAAGCAAATC AGAAGCTTTT TGTACCTGAA
    CCTCGCCCAG TTCCTAAAAA TGACGTTGGA TTTAAATATT CTGTGAAACA TCGGCCATTT
    CCTGAAGAGG TGGACAAGAT TCCTTTTGTG AAAGCAGATC TCAGCATTCC AGATTTGCAT
    GAAATAGTCA CTGAAGAATT AGAAGAGAGA CACGAAAAAT TAAGGAGTGG CATGGCCCAG
    CAGATTGCTT ATGAAATACA CCTTGAGCAA CAGAGTGAGG AGGAATTGCA GAAGAGAAGT
    TTTCCTGACC CAGTTACGGA TTGCAAGCCC CCGCCTCCTG CCCAGGAATT CCAAACAGCC
    CGCCTTTTTC TCTCACACTT TGGATTTTTG TCCTTAGAAG CACTGAAGGA ACCTGCAAAT
    AGTCGTCTAC CTCCTCACCT TATTGCACTT GATTCCACGA TACCTGGATT TTTTGATGAC
    ATTGGGTATC TGGATCTCTT GCCATGTCGT CCTTTTGACA CAGTTTTTAT TTTCTATATG
    AAGCCAGGTC AGAAAACGAA CCAAGAGATT TTAAAGAATG TGGAGTCTTC CAGAACTGTT
    CAGCCACATT TCCTAGAATT TTTGCTTTCC CTTGGCTGGT CAGTAGATGT GGGCAGACAC
    CCTGGTTGGA CTGGGCATGT TTCTACCAGT TGGTCTATTA ATTGTTGTGA TGATGGTGAA
    GGATCTCAAC AAGAAGTGAT TTCCTCTGAA GATATTGGAG CTAGCATTTT CAATGGACAG
    AAGAAGGTGC TGTATTATGC TGATGCCCTT ACAGAAATTG CTTTTGTGGT TCCTTCTCCT
    GTGGAGTCCT TAACTGATTC ATTGGAAAGT AACATCTCGG ACCAAGATAG TGATTCAAAT
    ATGGATCTTA TGCCAGGAAT TCTGAAACAG CCATCCCTGA CACTTGAGCT TTTCCCCAAT
    CATACAGACA ATCTTAATTC CTCACAGAGG CTCAGTCCCA GTTCCAGAAT GAGGAAGCTG
    CCTCAGGGTC GCCCTGTTCC TCCCCTTGGA CCTGAGACAA GAGTTTCTGT AGTCTGGGTG
    GAACGCTATG ATGATATAGA AAACTTTCCC CTCTCAGAGC TGATGACAGA GATCAGTACT
    GGTGTGGAAA CTACTGCAAA TAGTAGCACT TCACTGAGAT CTACAACTCT TGAAAAAGAA
    GTTCCTGTCA TCTTCATCCA CCCTTTAAAC ACTGGATTAT TCCGGATAAA AATTCAAGGA
    GCCACTGGAA AATTTAATAT GGTCATCCCT CTTGTGGATG GGATGATTGT CAGCAGGCGA
    GCTCTTGGCT TTCTGGTGAG GCAGACTGTA ATTAACATTT GTAGAAGAAA GAGACTGGAA
    AGTGACTCCT ACAGTCCCCC CCATGTCCGC CGGAAACAGA AAATCACCGA CATTGTCAAC
    AAGTACCGGA ACAAGCAGCT GGAGCCAGAG TTTTATACTT CACTTTTCCA GGAGGTTGGA
    CTCAAGAACT GCAGTTCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005260520.1
    CDS253..4773
    Translation

    Target ORF information:

    RefSeq Version XM_005260463.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005260463.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GCTCTTAATA CAGATTCAGC TGCTGGGCTC CTGATTCGCA GCATTCATCT CGTCACCCAA
    AGACTCAACT CCCAGTGGCG CCAAGACATG AGCATATCAC TGGCAGCTCT AGAGCTCCTC
    TCTGGCCTTG CAAAGGTAAA AGTGATGGTT GACTCAGGAG ACCGGAAGCG AGCCATCAGT
    TCTGTGTGCA CCTACATTGT TTATCAGTGT AGTCGGCCAG CTCCTTTACA CTCCAGGGAT
    CTGCACTCCA TGATAGTGGC AGCTTTTCAG TGTCTCTGTG TCTGGCTGAC AGAGCACCCT
    GATATGCTTG ATGAAAAGGA CTGCCTTAAG GAAGTACTGG AGATTGTGGA ACTGGGTATC
    TCAGGAAGTA AGTCCAAGAA CAATGAGCAA GAGGTCAAGT ACAAAGGAGA TAAGGAGCCA
    AACCCTGCAT CTATGAGGGT AAAGGATGCT GCTGAAGCCA CCCTAACATG CATTATGCAG
    TTGCTCGGCG CATTTCCTTC ACCTAGTGGT CCTGCCTCTC CTTGTAGTCT TGTGAATGAG
    ACCACTTTGA TTAAATACTC CAGGCTGCCA ACCATAAACA AGCATAGTTT CCGGTACTTT
    GTCTTGGATA ACAGTGTCAT CCTGGCAATG CTGGAACAAC CTCTTGGAAA TGAGCAGAAT
    GATTTTTTCC CCTCTGTCAC TGTGCTGGTC CGGGGAATGT CTGGAAGACT TGCTTGGGCA
    CAACAGCTTT GTCTTTTACC CAGAGGAGCA AAAGCAAATC AGAAGCTTTT TGTACCTGAA
    CCTCGCCCAG TTCCTAAAAA TGACGTTGGA TTTAAATATT CTGTGAAACA TCGGCCATTT
    CCTGAAGAGG TGGACAAGAT TCCTTTTGTG AAAGCAGATC TCAGCATTCC AGATTTGCAT
    GAAATAGTCA CTGAAGAATT AGAAGAGAGA CACGAAAAAT TAAGGAGTGG CATGGCCCAG
    CAGATTGCTT ATGAAATACA CCTTGAGCAA CAGAGTGAGG AGGAATTGCA GAAGAGAAGT
    TTTCCTGACC CAGTTACGGA TTGCAAGCCC CCGCCTCCTG CCCAGGAATT CCAAACAGCC
    CGCCTTTTTC TCTCACACTT TGGATTTTTG TCCTTAGAAG CACTGAAGGA ACCTGCAAAT
    AGTCGTCTAC CTCCTCACCT TATTGCACTT GATTCCACGA TACCTGGATT TTTTGATGAC
    ATTGGGTATC TGGATCTCTT GCCATGTCGT CCTTTTGACA CAGTTTTTAT TTTCTATATG
    AAGCCAGGTC AGAAAACGAA CCAAGAGATT TTAAAGAATG TGGAGTCTTC CAGAACTGTT
    CAGCCACATT TCCTAGAATT TTTGCTTTCC CTTGGCTGGT CAGTAGATGT GGGCAGACAC
    CCTGGTTGGA CTGGGCATGT TTCTACCAGT TGGTCTATTA ATTGTTGTGA TGATGGTGAA
    GGATCTCAAC AAGAAGTGAT TTCCTCTGAA GATATTGGAG CTAGCATTTT CAATGGACAG
    AAGAAGGTGC TGTATTATGC TGATGCCCTT ACAGAAATTG CTTTTGTGGT TCCTTCTCCT
    GTGGAGTCCT TAACTGATTC ATTGGAAAGT AACATCTCGG ACCAAGATAG TGATTCAAAT
    ATGGATCTTA TGCCAGGAAT TCTGAAACAG CCATCCCTGA CACTTGAGCT TTTCCCCAAT
    CATACAGACA ATCTTAATTC CTCACAGAGG CTCAGTCCCA GTTCCAGAAT GAGGAAGCTG
    CCTCAGGGTC GCCCTGTTCC TCCCCTTGGA CCTGAGACAA GAGTTTCTGT AGTCTGGGTG
    GAACGCTATG ATGATATAGA AAACTTTCCC CTCTCAGAGC TGATGACAGA GATCAGTACT
    GGTGTGGAAA CTACTGCAAA TAGTAGCACT TCACTGAGAT CTACAACTCT TGAAAAAGAA
    GTTCCTGTCA TCTTCATCCA CCCTTTAAAC ACTGGATTAT TCCGGATAAA AATTCAAGGA
    GCCACTGGAA AATTTAATAT GGTCATCCCT CTTGTGGATG GGATGATTGT CAGCAGGCGA
    GCTCTTGGCT TTCTGGTGAG GCAGACTGTA ATTAACATTT GTAGAAGAAA GAGACTGGAA
    AGTGACTCCT ACAGTCCCCC CCATGTCCGC CGGAAACAGA AAATCACCGA CATTGTCAAC
    AAGTACCGGA ACAAGCAGCT GGAGCCAGAG TTTTATACTT CACTTTTCCA GGAGGTTGGA
    CTCAAGAACT GCAGTTCTTA G

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53053
    Clone ID Related Accession (Same CDS sequence) XM_005260464.3 , XM_005260464.2
    Accession Version XM_005260464.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005260464.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GATTCAGCTG CTGGGCTCCT GATTCGCAGC ATTCATCTCG TCACCCAAAG ACTCAACTCC
    CAGTGGCGCC AAGACATGAG CATATCACTG GCAGCTCTAG AGCTCCTCTC TGGCCTTGCA
    AAGGTAAAAG TGATGGTTGA CTCAGGAGAC CGGAAGCGAG CCATCAGTTC TGTGTGCACC
    TACATTGTTT ATCAGTGTAG TCGGCCAGCT CCTTTACACT CCAGGGATCT GCACTCCATG
    ATAGTGGCAG CTTTTCAGTG TCTCTGTGTC TGGCTGACAG AGCACCCTGA TATGCTTGAT
    GAAAAGGACT GCCTTAAGGA AGTACTGGAG ATTGTGGAAC TGGGTATCTC AGGAAGTAAG
    TCCAAGAACA ATGAGCAAGA GGTCAAGTAC AAAGGAGATA AGGAGCCAAA CCCTGCATCT
    ATGAGGGTAA AGGATGCTGC TGAAGCCACC CTAACATGCA TTATGCAGTT GCTCGGCGCA
    TTTCCTTCAC CTAGTGGTCC TGCCTCTCCT TGTAGTCTTG TGAATGAGAC CACTTTGATT
    AAATACTCCA GGCTGCCAAC CATAAACAAG CATAGTTTCC GGTACTTTGT CTTGGATAAC
    AGTGTCATCC TGGCAATGCT GGAACAACCT CTTGGAAATG AGCAGAATGA TTTTTTCCCC
    TCTGTCACTG TGCTGGTCCG GGGAATGTCT GGAAGACTTG CTTGGGCACA ACAGCTTTGT
    CTTTTACCCA GAGGAGCAAA AGCAAATCAG AAGCTTTTTG TACCTGAACC TCGCCCAGTT
    CCTAAAAATG ACGTTGGATT TAAATATTCT GTGAAACATC GGCCATTTCC TGAAGAGGTG
    GACAAGATTC CTTTTGTGAA AGCAGATCTC AGCATTCCAG ATTTGCATGA AATAGTCACT
    GAAGAATTAG AAGAGAGACA CGAAAAATTA AGGAGTGGCA TGGCCCAGCA GATTGCTTAT
    GAAATACACC TTGAGCAACA GAGTGAGGAG GAATTGCAGA AGAGAAGTTT TCCTGACCCA
    GTTACGGATT GCAAGCCCCC GCCTCCTGCC CAGGAATTCC AAACAGCCCG CCTTTTTCTC
    TCACACTTTG GATTTTTGTC CTTAGAAGCA CTGAAGGAAC CTGCAAATAG TCGTCTACCT
    CCTCACCTTA TTGCACTTGA TTCCACGATA CCTGGATTTT TTGATGACAT TGGGTATCTG
    GATCTCTTGC CATGTCGTCC TTTTGACACA GTTTTTATTT TCTATATGAA GCCAGGTCAG
    AAAACGAACC AAGAGATTTT AAAGAATGTG GAGTCTTCCA GAACTGTTCA GCCACATTTC
    CTAGAATTTT TGCTTTCCCT TGGCTGGTCA GTAGATGTGG GCAGACACCC TGGTTGGACT
    GGGCATGTTT CTACCAGTTG GTCTATTAAT TGTTGTGATG ATGGTGAAGG ATCTCAACAA
    GAAGAAGTGA TTTCCTCTGA AGATATTGGA GCTAGCATTT TCAATGGACA GAAGAAGGTG
    CTGTATTATG CTGATGCCCT TACAGAAATT GCTTTTGTGG TTCCTTCTCC TGTGGAGTCC
    TTAACTGATT CATTGGAAAG TAACATCTCG GACCAAGATA GTGATTCAAA TATGGATCTT
    ATGCCAGGAA TTCTGAAACA GCCATCCCTG ACACTTGAGC TTTTCCCCAA TCATACAGAC
    AATCTTAATT CCTCACAGAG GCTCAGTCCC AGTTCCAGAA TGAGGAAGCT GCCTCAGGGT
    CGCCCTGTTC CTCCCCTTGG ACCTGAGACA AGAGTTTCTG TAGTCTGGGT GGAACGCTAT
    GATGATATAG AAAACTTTCC CCTCTCAGAG CTGATGACAG AGATCAGTAC TGGTGTGGAA
    ACTACTGCAA ATAGTAGCAC TTCACTGAGA TCTACAACTC TTGAAAAAGA AGTTCCTGTC
    ATCTTCATCC ACCCTTTAAA CACTGGATTA TTCCGGATAA AAATTCAAGG AGCCACTGGA
    AAATTTAATA TGGTCATCCC TCTTGTGGAT GGGATGATTG TCAGCAGGCG AGCTCTTGGC
    TTTCTGGTGA GGCAGACTGT AATTAACATT TGTAGAAGAA AGAGACTGGA AAGTGACTCC
    TACAGTCCCC CCCATGTCCG CCGGAAACAG AAAATCACCG ACATTGTCAA CAAGTACCGG
    AACAAGCAGC TGGAGCCAGA GTTTTATACT TCACTTTTCC AGGAGGTTGG ACTCAAGAAC
    TGCAGTTCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005260521.1
    CDS281..4792
    Translation

    Target ORF information:

    RefSeq Version XM_005260464.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005260464.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GATTCAGCTG CTGGGCTCCT GATTCGCAGC ATTCATCTCG TCACCCAAAG ACTCAACTCC
    CAGTGGCGCC AAGACATGAG CATATCACTG GCAGCTCTAG AGCTCCTCTC TGGCCTTGCA
    AAGGTAAAAG TGATGGTTGA CTCAGGAGAC CGGAAGCGAG CCATCAGTTC TGTGTGCACC
    TACATTGTTT ATCAGTGTAG TCGGCCAGCT CCTTTACACT CCAGGGATCT GCACTCCATG
    ATAGTGGCAG CTTTTCAGTG TCTCTGTGTC TGGCTGACAG AGCACCCTGA TATGCTTGAT
    GAAAAGGACT GCCTTAAGGA AGTACTGGAG ATTGTGGAAC TGGGTATCTC AGGAAGTAAG
    TCCAAGAACA ATGAGCAAGA GGTCAAGTAC AAAGGAGATA AGGAGCCAAA CCCTGCATCT
    ATGAGGGTAA AGGATGCTGC TGAAGCCACC CTAACATGCA TTATGCAGTT GCTCGGCGCA
    TTTCCTTCAC CTAGTGGTCC TGCCTCTCCT TGTAGTCTTG TGAATGAGAC CACTTTGATT
    AAATACTCCA GGCTGCCAAC CATAAACAAG CATAGTTTCC GGTACTTTGT CTTGGATAAC
    AGTGTCATCC TGGCAATGCT GGAACAACCT CTTGGAAATG AGCAGAATGA TTTTTTCCCC
    TCTGTCACTG TGCTGGTCCG GGGAATGTCT GGAAGACTTG CTTGGGCACA ACAGCTTTGT
    CTTTTACCCA GAGGAGCAAA AGCAAATCAG AAGCTTTTTG TACCTGAACC TCGCCCAGTT
    CCTAAAAATG ACGTTGGATT TAAATATTCT GTGAAACATC GGCCATTTCC TGAAGAGGTG
    GACAAGATTC CTTTTGTGAA AGCAGATCTC AGCATTCCAG ATTTGCATGA AATAGTCACT
    GAAGAATTAG AAGAGAGACA CGAAAAATTA AGGAGTGGCA TGGCCCAGCA GATTGCTTAT
    GAAATACACC TTGAGCAACA GAGTGAGGAG GAATTGCAGA AGAGAAGTTT TCCTGACCCA
    GTTACGGATT GCAAGCCCCC GCCTCCTGCC CAGGAATTCC AAACAGCCCG CCTTTTTCTC
    TCACACTTTG GATTTTTGTC CTTAGAAGCA CTGAAGGAAC CTGCAAATAG TCGTCTACCT
    CCTCACCTTA TTGCACTTGA TTCCACGATA CCTGGATTTT TTGATGACAT TGGGTATCTG
    GATCTCTTGC CATGTCGTCC TTTTGACACA GTTTTTATTT TCTATATGAA GCCAGGTCAG
    AAAACGAACC AAGAGATTTT AAAGAATGTG GAGTCTTCCA GAACTGTTCA GCCACATTTC
    CTAGAATTTT TGCTTTCCCT TGGCTGGTCA GTAGATGTGG GCAGACACCC TGGTTGGACT
    GGGCATGTTT CTACCAGTTG GTCTATTAAT TGTTGTGATG ATGGTGAAGG ATCTCAACAA
    GAAGAAGTGA TTTCCTCTGA AGATATTGGA GCTAGCATTT TCAATGGACA GAAGAAGGTG
    CTGTATTATG CTGATGCCCT TACAGAAATT GCTTTTGTGG TTCCTTCTCC TGTGGAGTCC
    TTAACTGATT CATTGGAAAG TAACATCTCG GACCAAGATA GTGATTCAAA TATGGATCTT
    ATGCCAGGAA TTCTGAAACA GCCATCCCTG ACACTTGAGC TTTTCCCCAA TCATACAGAC
    AATCTTAATT CCTCACAGAG GCTCAGTCCC AGTTCCAGAA TGAGGAAGCT GCCTCAGGGT
    CGCCCTGTTC CTCCCCTTGG ACCTGAGACA AGAGTTTCTG TAGTCTGGGT GGAACGCTAT
    GATGATATAG AAAACTTTCC CCTCTCAGAG CTGATGACAG AGATCAGTAC TGGTGTGGAA
    ACTACTGCAA ATAGTAGCAC TTCACTGAGA TCTACAACTC TTGAAAAAGA AGTTCCTGTC
    ATCTTCATCC ACCCTTTAAA CACTGGATTA TTCCGGATAA AAATTCAAGG AGCCACTGGA
    AAATTTAATA TGGTCATCCC TCTTGTGGAT GGGATGATTG TCAGCAGGCG AGCTCTTGGC
    TTTCTGGTGA GGCAGACTGT AATTAACATT TGTAGAAGAA AGAGACTGGA AAGTGACTCC
    TACAGTCCCC CCCATGTCCG CCGGAAACAG AAAATCACCG ACATTGTCAA CAAGTACCGG
    AACAAGCAGC TGGAGCCAGA GTTTTATACT TCACTTTTCC AGGAGGTTGG ACTCAAGAAC
    TGCAGTTCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53053
    Clone ID Related Accession (Same CDS sequence) XM_005260464.3 , XM_005260464.2
    Accession Version XM_005260464.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4512bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260464.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GATTCAGCTG CTGGGCTCCT GATTCGCAGC ATTCATCTCG TCACCCAAAG ACTCAACTCC
    CAGTGGCGCC AAGACATGAG CATATCACTG GCAGCTCTAG AGCTCCTCTC TGGCCTTGCA
    AAGGTAAAAG TGATGGTTGA CTCAGGAGAC CGGAAGCGAG CCATCAGTTC TGTGTGCACC
    TACATTGTTT ATCAGTGTAG TCGGCCAGCT CCTTTACACT CCAGGGATCT GCACTCCATG
    ATAGTGGCAG CTTTTCAGTG TCTCTGTGTC TGGCTGACAG AGCACCCTGA TATGCTTGAT
    GAAAAGGACT GCCTTAAGGA AGTACTGGAG ATTGTGGAAC TGGGTATCTC AGGAAGTAAG
    TCCAAGAACA ATGAGCAAGA GGTCAAGTAC AAAGGAGATA AGGAGCCAAA CCCTGCATCT
    ATGAGGGTAA AGGATGCTGC TGAAGCCACC CTAACATGCA TTATGCAGTT GCTCGGCGCA
    TTTCCTTCAC CTAGTGGTCC TGCCTCTCCT TGTAGTCTTG TGAATGAGAC CACTTTGATT
    AAATACTCCA GGCTGCCAAC CATAAACAAG CATAGTTTCC GGTACTTTGT CTTGGATAAC
    AGTGTCATCC TGGCAATGCT GGAACAACCT CTTGGAAATG AGCAGAATGA TTTTTTCCCC
    TCTGTCACTG TGCTGGTCCG GGGAATGTCT GGAAGACTTG CTTGGGCACA ACAGCTTTGT
    CTTTTACCCA GAGGAGCAAA AGCAAATCAG AAGCTTTTTG TACCTGAACC TCGCCCAGTT
    CCTAAAAATG ACGTTGGATT TAAATATTCT GTGAAACATC GGCCATTTCC TGAAGAGGTG
    GACAAGATTC CTTTTGTGAA AGCAGATCTC AGCATTCCAG ATTTGCATGA AATAGTCACT
    GAAGAATTAG AAGAGAGACA CGAAAAATTA AGGAGTGGCA TGGCCCAGCA GATTGCTTAT
    GAAATACACC TTGAGCAACA GAGTGAGGAG GAATTGCAGA AGAGAAGTTT TCCTGACCCA
    GTTACGGATT GCAAGCCCCC GCCTCCTGCC CAGGAATTCC AAACAGCCCG CCTTTTTCTC
    TCACACTTTG GATTTTTGTC CTTAGAAGCA CTGAAGGAAC CTGCAAATAG TCGTCTACCT
    CCTCACCTTA TTGCACTTGA TTCCACGATA CCTGGATTTT TTGATGACAT TGGGTATCTG
    GATCTCTTGC CATGTCGTCC TTTTGACACA GTTTTTATTT TCTATATGAA GCCAGGTCAG
    AAAACGAACC AAGAGATTTT AAAGAATGTG GAGTCTTCCA GAACTGTTCA GCCACATTTC
    CTAGAATTTT TGCTTTCCCT TGGCTGGTCA GTAGATGTGG GCAGACACCC TGGTTGGACT
    GGGCATGTTT CTACCAGTTG GTCTATTAAT TGTTGTGATG ATGGTGAAGG ATCTCAACAA
    GAAGAAGTGA TTTCCTCTGA AGATATTGGA GCTAGCATTT TCAATGGACA GAAGAAGGTG
    CTGTATTATG CTGATGCCCT TACAGAAATT GCTTTTGTGG TTCCTTCTCC TGTGGAGTCC
    TTAACTGATT CATTGGAAAG TAACATCTCG GACCAAGATA GTGATTCAAA TATGGATCTT
    ATGCCAGGAA TTCTGAAACA GCCATCCCTG ACACTTGAGC TTTTCCCCAA TCATACAGAC
    AATCTTAATT CCTCACAGAG GCTCAGTCCC AGTTCCAGAA TGAGGAAGCT GCCTCAGGGT
    CGCCCTGTTC CTCCCCTTGG ACCTGAGACA AGAGTTTCTG TAGTCTGGGT GGAACGCTAT
    GATGATATAG AAAACTTTCC CCTCTCAGAG CTGATGACAG AGATCAGTAC TGGTGTGGAA
    ACTACTGCAA ATAGTAGCAC TTCACTGAGA TCTACAACTC TTGAAAAAGA AGTTCCTGTC
    ATCTTCATCC ACCCTTTAAA CACTGGATTA TTCCGGATAA AAATTCAAGG AGCCACTGGA
    AAATTTAATA TGGTCATCCC TCTTGTGGAT GGGATGATTG TCAGCAGGCG AGCTCTTGGC
    TTTCTGGTGA GGCAGACTGT AATTAACATT TGTAGAAGAA AGAGACTGGA AAGTGACTCC
    TACAGTCCCC CCCATGTCCG CCGGAAACAG AAAATCACCG ACATTGTCAA CAAGTACCGG
    AACAAGCAGC TGGAGCCAGA GTTTTATACT TCACTTTTCC AGGAGGTTGG ACTCAAGAAC
    TGCAGTTCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005260521.1
    CDS253..4764
    Translation

    Target ORF information:

    RefSeq Version XM_005260464.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005260464.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCAT CTTTTATCCA AATTCTTCTT
    TCTTATAAAT CTTCCATTAC AACACAAGCT AGCATGGAGT TTCGACGGAA AGGGTCACAA
    ATGTCCACAG ACACCATGGT TTCCAATCCT ATGTTTGATG CAAGTGAATT TCCTGATAAC
    TATGAAGCAG GAAGAGCTGA GGCTTGTGGG ACACTGTGTA GGATTTTTTG TAGCAAGAAG
    ACTGGAGAAG AGATTCTGCC AGCTTATTTA TCCAGATTTT ACATGCTTTT AATTCAAGGT
    TTGCAGATAA ATGATTATGT GTGCCATCCT GTCTTGGCCA GCGTTATTCT AAACTCTCCT
    CCTTTGTTCT GCTGTGACTT GAAAGGGATT GATGTTGTGG TTCCTTACTT TATTTCAGCT
    CTTGAAACCA TTTTGCCTGA CAGAGAACTC TCAAAATTCA AAAGCTATGT AAATCCAACA
    GAATTGCGAA GATCCTCCAT TAATATCCTG CTTTCTTTGT TGCCCCTCCC TCATCATTTT
    GGCACAGTCA AATCTGAGGT GGTCCTGGAA GGAAAGTTTA GTAACGATGA CAGCTCTTCT
    TATGATAAAC CAATAACTTT TCTGTCCCTG AAGTTGAGAC TTGTGAATAT ATTAATAGGT
    GCCTTGCAAA CTGAAACGGA CCCCAACAAC ACCCAAATGA TATTAGGGGC AATGTTAAAT
    ATTGTTCAAG ATTCAGCACT TTTGGAAGCC ATTGGTTGCC AGATGGAGAT GGGTGGTGGA
    GAAAATAACC TGAAGAGTCA TAGTCGCACC AATAGTGGTA TTAGTTCAGC AAGTGGTGGA
    AGCACGGAGC CCACGACTCC CGATAGTGAG AGACCTGCTC AAGCTCTCTT AAGAGATTAT
    GATTCAGCTG CTGGGCTCCT GATTCGCAGC ATTCATCTCG TCACCCAAAG ACTCAACTCC
    CAGTGGCGCC AAGACATGAG CATATCACTG GCAGCTCTAG AGCTCCTCTC TGGCCTTGCA
    AAGGTAAAAG TGATGGTTGA CTCAGGAGAC CGGAAGCGAG CCATCAGTTC TGTGTGCACC
    TACATTGTTT ATCAGTGTAG TCGGCCAGCT CCTTTACACT CCAGGGATCT GCACTCCATG
    ATAGTGGCAG CTTTTCAGTG TCTCTGTGTC TGGCTGACAG AGCACCCTGA TATGCTTGAT
    GAAAAGGACT GCCTTAAGGA AGTACTGGAG ATTGTGGAAC TGGGTATCTC AGGAAGTAAG
    TCCAAGAACA ATGAGCAAGA GGTCAAGTAC AAAGGAGATA AGGAGCCAAA CCCTGCATCT
    ATGAGGGTAA AGGATGCTGC TGAAGCCACC CTAACATGCA TTATGCAGTT GCTCGGCGCA
    TTTCCTTCAC CTAGTGGTCC TGCCTCTCCT TGTAGTCTTG TGAATGAGAC CACTTTGATT
    AAATACTCCA GGCTGCCAAC CATAAACAAG CATAGTTTCC GGTACTTTGT CTTGGATAAC
    AGTGTCATCC TGGCAATGCT GGAACAACCT CTTGGAAATG AGCAGAATGA TTTTTTCCCC
    TCTGTCACTG TGCTGGTCCG GGGAATGTCT GGAAGACTTG CTTGGGCACA ACAGCTTTGT
    CTTTTACCCA GAGGAGCAAA AGCAAATCAG AAGCTTTTTG TACCTGAACC TCGCCCAGTT
    CCTAAAAATG ACGTTGGATT TAAATATTCT GTGAAACATC GGCCATTTCC TGAAGAGGTG
    GACAAGATTC CTTTTGTGAA AGCAGATCTC AGCATTCCAG ATTTGCATGA AATAGTCACT
    GAAGAATTAG AAGAGAGACA CGAAAAATTA AGGAGTGGCA TGGCCCAGCA GATTGCTTAT
    GAAATACACC TTGAGCAACA GAGTGAGGAG GAATTGCAGA AGAGAAGTTT TCCTGACCCA
    GTTACGGATT GCAAGCCCCC GCCTCCTGCC CAGGAATTCC AAACAGCCCG CCTTTTTCTC
    TCACACTTTG GATTTTTGTC CTTAGAAGCA CTGAAGGAAC CTGCAAATAG TCGTCTACCT
    CCTCACCTTA TTGCACTTGA TTCCACGATA CCTGGATTTT TTGATGACAT TGGGTATCTG
    GATCTCTTGC CATGTCGTCC TTTTGACACA GTTTTTATTT TCTATATGAA GCCAGGTCAG
    AAAACGAACC AAGAGATTTT AAAGAATGTG GAGTCTTCCA GAACTGTTCA GCCACATTTC
    CTAGAATTTT TGCTTTCCCT TGGCTGGTCA GTAGATGTGG GCAGACACCC TGGTTGGACT
    GGGCATGTTT CTACCAGTTG GTCTATTAAT TGTTGTGATG ATGGTGAAGG ATCTCAACAA
    GAAGAAGTGA TTTCCTCTGA AGATATTGGA GCTAGCATTT TCAATGGACA GAAGAAGGTG
    CTGTATTATG CTGATGCCCT TACAGAAATT GCTTTTGTGG TTCCTTCTCC TGTGGAGTCC
    TTAACTGATT CATTGGAAAG TAACATCTCG GACCAAGATA GTGATTCAAA TATGGATCTT
    ATGCCAGGAA TTCTGAAACA GCCATCCCTG ACACTTGAGC TTTTCCCCAA TCATACAGAC
    AATCTTAATT CCTCACAGAG GCTCAGTCCC AGTTCCAGAA TGAGGAAGCT GCCTCAGGGT
    CGCCCTGTTC CTCCCCTTGG ACCTGAGACA AGAGTTTCTG TAGTCTGGGT GGAACGCTAT
    GATGATATAG AAAACTTTCC CCTCTCAGAG CTGATGACAG AGATCAGTAC TGGTGTGGAA
    ACTACTGCAA ATAGTAGCAC TTCACTGAGA TCTACAACTC TTGAAAAAGA AGTTCCTGTC
    ATCTTCATCC ACCCTTTAAA CACTGGATTA TTCCGGATAA AAATTCAAGG AGCCACTGGA
    AAATTTAATA TGGTCATCCC TCTTGTGGAT GGGATGATTG TCAGCAGGCG AGCTCTTGGC
    TTTCTGGTGA GGCAGACTGT AATTAACATT TGTAGAAGAA AGAGACTGGA AAGTGACTCC
    TACAGTCCCC CCCATGTCCG CCGGAAACAG AAAATCACCG ACATTGTCAA CAAGTACCGG
    AACAAGCAGC TGGAGCCAGA GTTTTATACT TCACTTTTCC AGGAGGTTGG ACTCAAGAAC
    TGCAGTTCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53054
    Clone ID Related Accession (Same CDS sequence) XM_005260465.3 , XM_005260465.2
    Accession Version XM_005260465.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_005260465.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC CCAAAGACTC AACTCCCAGT GGCGCCAAGA CATGAGCATA
    TCACTGGCAG CTCTAGAGCT CCTCTCTGGC CTTGCAAAGG TAAAAGTGAT GGTTGACTCA
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCACCTACA TTGTTTATCA GTGTAGTCGG
    CCAGCTCCTT TACACTCCAG GGATCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA CCCTGATATG CTTGATGAAA AGGACTGCCT TAAGGAAGTA
    CTGGAGATTG TGGAACTGGG TATCTCAGGA AGTAAGTCCA AGAACAATGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGCATTAT GCAGTTGCTC GGCGCATTTC CTTCACCTAG TGGTCCTGCC
    TCTCCTTGTA GTCTTGTGAA TGAGACCACT TTGATTAAAT ACTCCAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAA
    CAACCTCTTG GAAATGAGCA GAATGATTTT TTCCCCTCTG TCACTGTGCT GGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAG CTTTGTCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGC CCAGTTCCTA AAAATGACGT TGGATTTAAA
    TATTCTGTGA AACATCGGCC ATTTCCTGAA GAGGTGGACA AGATTCCTTT TGTGAAAGCA
    GATCTCAGCA TTCCAGATTT GCATGAAATA GTCACTGAAG AATTAGAAGA GAGACACGAA
    AAATTAAGGA GTGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA GCAACAGAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GACCCAGTTA CGGATTGCAA GCCCCCGCCT
    CCTGCCCAGG AATTCCAAAC AGCCCGCCTT TTTCTCTCAC ACTTTGGATT TTTGTCCTTA
    GAAGCACTGA AGGAACCTGC AAATAGTCGT CTACCTCCTC ACCTTATTGC ACTTGATTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCTTTT
    GACACAGTTT TTATTTTCTA TATGAAGCCA GGTCAGAAAA CGAACCAAGA GATTTTAAAG
    AATGTGGAGT CTTCCAGAAC TGTTCAGCCA CATTTCCTAG AATTTTTGCT TTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCTGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAATTGTT GTGATGATGG TGAAGGATCT CAACAAGAAG AAGTGATTTC CTCTGAAGAT
    ATTGGAGCTA GCATTTTCAA TGGACAGAAG AAGGTGCTGT ATTATGCTGA TGCCCTTACA
    GAAATTGCTT TTGTGGTTCC TTCTCCTGTG GAGTCCTTAA CTGATTCATT GGAAAGTAAC
    ATCTCGGACC AAGATAGTGA TTCAAATATG GATCTTATGC CAGGAATTCT GAAACAGCCA
    TCCCTGACAC TTGAGCTTTT CCCCAATCAT ACAGACAATC TTAATTCCTC ACAGAGGCTC
    AGTCCCAGTT CCAGAATGAG GAAGCTGCCT CAGGGTCGCC CTGTTCCTCC CCTTGGACCT
    GAGACAAGAG TTTCTGTAGT CTGGGTGGAA CGCTATGATG ATATAGAAAA CTTTCCCCTC
    TCAGAGCTGA TGACAGAGAT CAGTACTGGT GTGGAAACTA CTGCAAATAG TAGCACTTCA
    CTGAGATCTA CAACTCTTGA AAAAGAAGTT CCTGTCATCT TCATCCACCC TTTAAACACT
    GGATTATTCC GGATAAAAAT TCAAGGAGCC ACTGGAAAAT TTAATATGGT CATCCCTCTT
    GTGGATGGGA TGATTGTCAG CAGGCGAGCT CTTGGCTTTC TGGTGAGGCA GACTGTAATT
    AACATTTGTA GAAGAAAGAG ACTGGAAAGT GACTCCTACA GTCCCCCCCA TGTCCGCCGG
    AAACAGAAAA TCACCGACAT TGTCAACAAG TACCGGAACA AGCAGCTGGA GCCAGAGTTT
    TATACTTCAC TTTTCCAGGA GGTTGGACTC AAGAACTGCA GTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005260522.1
    CDS281..4768
    Translation

    Target ORF information:

    RefSeq Version XM_005260465.3
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic subunit beta (RALGAPB), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005260465.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC CCAAAGACTC AACTCCCAGT GGCGCCAAGA CATGAGCATA
    TCACTGGCAG CTCTAGAGCT CCTCTCTGGC CTTGCAAAGG TAAAAGTGAT GGTTGACTCA
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCACCTACA TTGTTTATCA GTGTAGTCGG
    CCAGCTCCTT TACACTCCAG GGATCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA CCCTGATATG CTTGATGAAA AGGACTGCCT TAAGGAAGTA
    CTGGAGATTG TGGAACTGGG TATCTCAGGA AGTAAGTCCA AGAACAATGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGCATTAT GCAGTTGCTC GGCGCATTTC CTTCACCTAG TGGTCCTGCC
    TCTCCTTGTA GTCTTGTGAA TGAGACCACT TTGATTAAAT ACTCCAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAA
    CAACCTCTTG GAAATGAGCA GAATGATTTT TTCCCCTCTG TCACTGTGCT GGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAG CTTTGTCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGC CCAGTTCCTA AAAATGACGT TGGATTTAAA
    TATTCTGTGA AACATCGGCC ATTTCCTGAA GAGGTGGACA AGATTCCTTT TGTGAAAGCA
    GATCTCAGCA TTCCAGATTT GCATGAAATA GTCACTGAAG AATTAGAAGA GAGACACGAA
    AAATTAAGGA GTGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA GCAACAGAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GACCCAGTTA CGGATTGCAA GCCCCCGCCT
    CCTGCCCAGG AATTCCAAAC AGCCCGCCTT TTTCTCTCAC ACTTTGGATT TTTGTCCTTA
    GAAGCACTGA AGGAACCTGC AAATAGTCGT CTACCTCCTC ACCTTATTGC ACTTGATTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCTTTT
    GACACAGTTT TTATTTTCTA TATGAAGCCA GGTCAGAAAA CGAACCAAGA GATTTTAAAG
    AATGTGGAGT CTTCCAGAAC TGTTCAGCCA CATTTCCTAG AATTTTTGCT TTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCTGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAATTGTT GTGATGATGG TGAAGGATCT CAACAAGAAG AAGTGATTTC CTCTGAAGAT
    ATTGGAGCTA GCATTTTCAA TGGACAGAAG AAGGTGCTGT ATTATGCTGA TGCCCTTACA
    GAAATTGCTT TTGTGGTTCC TTCTCCTGTG GAGTCCTTAA CTGATTCATT GGAAAGTAAC
    ATCTCGGACC AAGATAGTGA TTCAAATATG GATCTTATGC CAGGAATTCT GAAACAGCCA
    TCCCTGACAC TTGAGCTTTT CCCCAATCAT ACAGACAATC TTAATTCCTC ACAGAGGCTC
    AGTCCCAGTT CCAGAATGAG GAAGCTGCCT CAGGGTCGCC CTGTTCCTCC CCTTGGACCT
    GAGACAAGAG TTTCTGTAGT CTGGGTGGAA CGCTATGATG ATATAGAAAA CTTTCCCCTC
    TCAGAGCTGA TGACAGAGAT CAGTACTGGT GTGGAAACTA CTGCAAATAG TAGCACTTCA
    CTGAGATCTA CAACTCTTGA AAAAGAAGTT CCTGTCATCT TCATCCACCC TTTAAACACT
    GGATTATTCC GGATAAAAAT TCAAGGAGCC ACTGGAAAAT TTAATATGGT CATCCCTCTT
    GTGGATGGGA TGATTGTCAG CAGGCGAGCT CTTGGCTTTC TGGTGAGGCA GACTGTAATT
    AACATTTGTA GAAGAAAGAG ACTGGAAAGT GACTCCTACA GTCCCCCCCA TGTCCGCCGG
    AAACAGAAAA TCACCGACAT TGTCAACAAG TACCGGAACA AGCAGCTGGA GCCAGAGTTT
    TATACTTCAC TTTTCCAGGA GGTTGGACTC AAGAACTGCA GTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu53054
    Clone ID Related Accession (Same CDS sequence) XM_005260465.3 , XM_005260465.2
    Accession Version XM_005260465.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4488bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product ral GTPase-activating protein subunit beta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000020.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005260465.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC CCAAAGACTC AACTCCCAGT GGCGCCAAGA CATGAGCATA
    TCACTGGCAG CTCTAGAGCT CCTCTCTGGC CTTGCAAAGG TAAAAGTGAT GGTTGACTCA
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCACCTACA TTGTTTATCA GTGTAGTCGG
    CCAGCTCCTT TACACTCCAG GGATCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA CCCTGATATG CTTGATGAAA AGGACTGCCT TAAGGAAGTA
    CTGGAGATTG TGGAACTGGG TATCTCAGGA AGTAAGTCCA AGAACAATGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGCATTAT GCAGTTGCTC GGCGCATTTC CTTCACCTAG TGGTCCTGCC
    TCTCCTTGTA GTCTTGTGAA TGAGACCACT TTGATTAAAT ACTCCAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAA
    CAACCTCTTG GAAATGAGCA GAATGATTTT TTCCCCTCTG TCACTGTGCT GGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAG CTTTGTCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGC CCAGTTCCTA AAAATGACGT TGGATTTAAA
    TATTCTGTGA AACATCGGCC ATTTCCTGAA GAGGTGGACA AGATTCCTTT TGTGAAAGCA
    GATCTCAGCA TTCCAGATTT GCATGAAATA GTCACTGAAG AATTAGAAGA GAGACACGAA
    AAATTAAGGA GTGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA GCAACAGAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GACCCAGTTA CGGATTGCAA GCCCCCGCCT
    CCTGCCCAGG AATTCCAAAC AGCCCGCCTT TTTCTCTCAC ACTTTGGATT TTTGTCCTTA
    GAAGCACTGA AGGAACCTGC AAATAGTCGT CTACCTCCTC ACCTTATTGC ACTTGATTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCTTTT
    GACACAGTTT TTATTTTCTA TATGAAGCCA GGTCAGAAAA CGAACCAAGA GATTTTAAAG
    AATGTGGAGT CTTCCAGAAC TGTTCAGCCA CATTTCCTAG AATTTTTGCT TTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCTGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAATTGTT GTGATGATGG TGAAGGATCT CAACAAGAAG AAGTGATTTC CTCTGAAGAT
    ATTGGAGCTA GCATTTTCAA TGGACAGAAG AAGGTGCTGT ATTATGCTGA TGCCCTTACA
    GAAATTGCTT TTGTGGTTCC TTCTCCTGTG GAGTCCTTAA CTGATTCATT GGAAAGTAAC
    ATCTCGGACC AAGATAGTGA TTCAAATATG GATCTTATGC CAGGAATTCT GAAACAGCCA
    TCCCTGACAC TTGAGCTTTT CCCCAATCAT ACAGACAATC TTAATTCCTC ACAGAGGCTC
    AGTCCCAGTT CCAGAATGAG GAAGCTGCCT CAGGGTCGCC CTGTTCCTCC CCTTGGACCT
    GAGACAAGAG TTTCTGTAGT CTGGGTGGAA CGCTATGATG ATATAGAAAA CTTTCCCCTC
    TCAGAGCTGA TGACAGAGAT CAGTACTGGT GTGGAAACTA CTGCAAATAG TAGCACTTCA
    CTGAGATCTA CAACTCTTGA AAAAGAAGTT CCTGTCATCT TCATCCACCC TTTAAACACT
    GGATTATTCC GGATAAAAAT TCAAGGAGCC ACTGGAAAAT TTAATATGGT CATCCCTCTT
    GTGGATGGGA TGATTGTCAG CAGGCGAGCT CTTGGCTTTC TGGTGAGGCA GACTGTAATT
    AACATTTGTA GAAGAAAGAG ACTGGAAAGT GACTCCTACA GTCCCCCCCA TGTCCGCCGG
    AAACAGAAAA TCACCGACAT TGTCAACAAG TACCGGAACA AGCAGCTGGA GCCAGAGTTT
    TATACTTCAC TTTTCCAGGA GGTTGGACTC AAGAACTGCA GTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005260522.1
    CDS253..4740
    Translation

    Target ORF information:

    RefSeq Version XM_005260465.2
    Organism Homo sapiens(human)
    Definition Homo sapiens Ral GTPase activating protein non-catalytic beta subunit (RALGAPB), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005260465.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGTACTCTG AGTGGAGGTC ACTGCATTTG GTGATTCAGA ATGATCAAGG CCATACCAGT 
    GTGCTGCACA GCTATCCAGA GAGCGTTGGA CGAGAGGTGG CAAATGCTGT AGTCCGTCCT
    CTTGGGCAGG TGTTAGGTAC CCCTTCAGTG GCTGGTAGTG AGAATTTGTT AAAAACTGAC
    AAAGAAGTAA AATGGACCAT GGAAGTAATT TGCTATGGAC TGACCCTTCC ATTGGATGGA
    GAGACTGTAA AATATTGCGT TGATGTATAT ACAGACTGGA TTATGGCTTT AGTGTTGCCA
    AAAGATTCTA TTCCATTGCC AGTTATTAAA GAGCCTAATC AATATGTTCA AACTATACTA
    AAACACCTAC AGAATCTTTT TGTACCAAGA CAGGAACAGG GTTCCAGTCA GATTCGACTA
    TGCTTACAGG TCCTGAGAGC CATTCAGAAA CTGGCCCGTG AGTCATCTCT CATGGCCCGA
    GAAACTTGGG AAGTCTTACT GTTGTTTCTT CTGCAGATTA ACGACATACT TCTGGCCCCA
    CCAACTGTTC AAGGTGGCAT TGCTGAGAAT CTAGCAGAGA AGTTGATTGG TGTTCTCTTT
    GAGGTGTGGT TACTAGCTTG TACTCGGTGC TTCCCAACAC CTCCTTATTG GAAAACAGCC
    AAGGAGATGG TGGCTAACTG GAGGCATCAC CCAGCAGTGG TGGAGCAGTG GAGCAAGGTC
    ATTTGTGCAC TCACTTCCAG ATTGCTACGC TTTACATATG GTCCTTCATT TCCTGCATTT
    AAAGTTCCCG ATGAAGATGC CAGTCTGATC CCTCCAGAAA TGGATAATGA GTGTGTTGCA
    CAGACATGGT TTCGCTTTTT ACACATGTTA AGTAATCCTG TGGATTTGAG TAACCCAGCT
    ATTATAAGCT CTACTCCCAA ATTTCAGGAA CAGTTCTTGA ATGTGAGCGG AATGCCGCAA
    GAATTGAATC AGTATCCCTG CCTTAAACAT CTGCCTCAAA TATTTTTTCG TGCCATGCGT
    GGAATCAGCT GTCTGGTGGA TGCATTCTTA GGTATTTCTA GACCCCGATC AGACAGTGCT
    CCCCCAACAC CCGTGAATAG ATTAAGTATG CCTCAAAGTG CTGCTGTCAG TACCACCCCC
    CCACATAACC GGAGGCACCG GGCTGTTACT GTGAATAAGG CCACCATGAA GACAAGCACA
    GTTAGTACTG CTCATGCCTC TAAAGTTCAG CACCAGACGT CCTCCACCTC TCCTCTGTCA
    AGTCCAAATC AGACTAGTTC AGAACCCCGG CCACTGCCTG CCCCTCGGAG ACCAAAGGTT
    AACAGCATCT TGAATCTCTT TGGATCATGG TTATTTGATG CAGCATTTGT TCACTGTAAA
    CTTCATAATG GGATAAACAG AGACAGCAGC ATGACTGCCA TTACAACACA AGCTAGCATG
    GAGTTTCGAC GGAAAGGGTC ACAAATGTCC ACAGACACCA TGGTTTCCAA TCCTATGTTT
    GATGCAAGTG AATTTCCTGA TAACTATGAA GCAGGAAGAG CTGAGGCTTG TGGGACACTG
    TGTAGGATTT TTTGTAGCAA GAAGACTGGA GAAGAGATTC TGCCAGCTTA TTTATCCAGA
    TTTTACATGC TTTTAATTCA AGGTTTGCAG ATAAATGATT ATGTGTGCCA TCCTGTCTTG
    GCCAGCGTTA TTCTAAACTC TCCTCCTTTG TTCTGCTGTG ACTTGAAAGG GATTGATGTT
    GTGGTTCCTT ACTTTATTTC AGCTCTTGAA ACCATTTTGC CTGACAGAGA ACTCTCAAAA
    TTCAAAAGCT ATGTAAATCC AACAGAATTG CGAAGATCCT CCATTAATAT CCTGCTTTCT
    TTGTTGCCCC TCCCTCATCA TTTTGGCACA GTCAAATCTG AGGTGGTCCT GGAAGGAAAG
    TTTAGTAACG ATGACAGCTC TTCTTATGAT AAACCAATAA CTTTTCTGTC CCTGAAGTTG
    AGACTTGTGA ATATATTAAT AGGTGCCTTG CAAACTGAAA CGGACCCCAA CAACACCCAA
    ATGATATTAG GGGCAATGTT AAATATTGTT CAAGATTCAG CACTTTTGGA AGCCATTGGT
    TGCCAGATGG AGATGGGTGG TGGAGAAAAT AACCTGAAGA GTCATAGTCG CACCAATAGT
    GGTATTAGTT CAGCAAGTGG TGGAAGCACG GAGCCCACGA CTCCCGATAG TGAGAGACCT
    GCTCAAGCTC TCTTAAGAGA TTATGCTCTT AATACAGATT CAGCTGCTGG GCTCCTGATT
    CGCAGCATTC ATCTCGTCAC CCAAAGACTC AACTCCCAGT GGCGCCAAGA CATGAGCATA
    TCACTGGCAG CTCTAGAGCT CCTCTCTGGC CTTGCAAAGG TAAAAGTGAT GGTTGACTCA
    GGAGACCGGA AGCGAGCCAT CAGTTCTGTG TGCACCTACA TTGTTTATCA GTGTAGTCGG
    CCAGCTCCTT TACACTCCAG GGATCTGCAC TCCATGATAG TGGCAGCTTT TCAGTGTCTC
    TGTGTCTGGC TGACAGAGCA CCCTGATATG CTTGATGAAA AGGACTGCCT TAAGGAAGTA
    CTGGAGATTG TGGAACTGGG TATCTCAGGA AGTAAGTCCA AGAACAATGA GCAAGAGGTC
    AAGTACAAAG GAGATAAGGA GCCAAACCCT GCATCTATGA GGGTAAAGGA TGCTGCTGAA
    GCCACCCTAA CATGCATTAT GCAGTTGCTC GGCGCATTTC CTTCACCTAG TGGTCCTGCC
    TCTCCTTGTA GTCTTGTGAA TGAGACCACT TTGATTAAAT ACTCCAGGCT GCCAACCATA
    AACAAGCATA GTTTCCGGTA CTTTGTCTTG GATAACAGTG TCATCCTGGC AATGCTGGAA
    CAACCTCTTG GAAATGAGCA GAATGATTTT TTCCCCTCTG TCACTGTGCT GGTCCGGGGA
    ATGTCTGGAA GACTTGCTTG GGCACAACAG CTTTGTCTTT TACCCAGAGG AGCAAAAGCA
    AATCAGAAGC TTTTTGTACC TGAACCTCGC CCAGTTCCTA AAAATGACGT TGGATTTAAA
    TATTCTGTGA AACATCGGCC ATTTCCTGAA GAGGTGGACA AGATTCCTTT TGTGAAAGCA
    GATCTCAGCA TTCCAGATTT GCATGAAATA GTCACTGAAG AATTAGAAGA GAGACACGAA
    AAATTAAGGA GTGGCATGGC CCAGCAGATT GCTTATGAAA TACACCTTGA GCAACAGAGT
    GAGGAGGAAT TGCAGAAGAG AAGTTTTCCT GACCCAGTTA CGGATTGCAA GCCCCCGCCT
    CCTGCCCAGG AATTCCAAAC AGCCCGCCTT TTTCTCTCAC ACTTTGGATT TTTGTCCTTA
    GAAGCACTGA AGGAACCTGC AAATAGTCGT CTACCTCCTC ACCTTATTGC ACTTGATTCC
    ACGATACCTG GATTTTTTGA TGACATTGGG TATCTGGATC TCTTGCCATG TCGTCCTTTT
    GACACAGTTT TTATTTTCTA TATGAAGCCA GGTCAGAAAA CGAACCAAGA GATTTTAAAG
    AATGTGGAGT CTTCCAGAAC TGTTCAGCCA CATTTCCTAG AATTTTTGCT TTCCCTTGGC
    TGGTCAGTAG ATGTGGGCAG ACACCCTGGT TGGACTGGGC ATGTTTCTAC CAGTTGGTCT
    ATTAATTGTT GTGATGATGG TGAAGGATCT CAACAAGAAG AAGTGATTTC CTCTGAAGAT
    ATTGGAGCTA GCATTTTCAA TGGACAGAAG AAGGTGCTGT ATTATGCTGA TGCCCTTACA
    GAAATTGCTT TTGTGGTTCC TTCTCCTGTG GAGTCCTTAA CTGATTCATT GGAAAGTAAC
    ATCTCGGACC AAGATAGTGA TTCAAATATG GATCTTATGC CAGGAATTCT GAAACAGCCA
    TCCCTGACAC TTGAGCTTTT CCCCAATCAT ACAGACAATC TTAATTCCTC ACAGAGGCTC
    AGTCCCAGTT CCAGAATGAG GAAGCTGCCT CAGGGTCGCC CTGTTCCTCC CCTTGGACCT
    GAGACAAGAG TTTCTGTAGT CTGGGTGGAA CGCTATGATG ATATAGAAAA CTTTCCCCTC
    TCAGAGCTGA TGACAGAGAT CAGTACTGGT GTGGAAACTA CTGCAAATAG TAGCACTTCA
    CTGAGATCTA CAACTCTTGA AAAAGAAGTT CCTGTCATCT TCATCCACCC TTTAAACACT
    GGATTATTCC GGATAAAAAT TCAAGGAGCC ACTGGAAAAT TTAATATGGT CATCCCTCTT
    GTGGATGGGA TGATTGTCAG CAGGCGAGCT CTTGGCTTTC TGGTGAGGCA GACTGTAATT
    AACATTTGTA GAAGAAAGAG ACTGGAAAGT GACTCCTACA GTCCCCCCCA TGTCCGCCGG
    AAACAGAAAA TCACCGACAT TGTCAACAAG TACCGGAACA AGCAGCTGGA GCCAGAGTTT
    TATACTTCAC TTTTCCAGGA GGTTGGACTC AAGAACTGCA GTTCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.