PSMB10 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PSMB10
Entrez Gene ID	5699
Full Name	proteasome subunit beta 10
Synonyms	LMP10,MECL1,beta2i
General protein information	Preferred Names proteasome subunit beta 10 Names proteasome subunit beta type-10 low molecular mass protein 10 macropain subunit MECl-1 multicatalytic endopeptidase complex subunit MECl-1 proteasome (prosome, macropain) subunit, beta type, 10 proteasome MECl-1 proteasome catalytic subunit 2i proteasome subunit MECL1 proteasome subunit beta 7i proteasome subunit beta-2i
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q22.1
Summary	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. Proteolytic processing is required to generate a mature subunit. Expression of this gene is induced by gamma interferon, and this gene product replaces catalytic subunit 2 (proteasome beta 7 subunit) in the immunoproteasome. [provided by RefSeq, Jul 2008].
Disorder MIM:	176847

mRNA and Protein(s)

mRNA	Protein	Name
NM_002801.4	NP_002792.1	proteasome subunit beta type-10
NM_002801.3	NP_002792.1	proteasome subunit beta type-10 precursor

Pathways from BioSystems

Homologies

	PSMB10	XP_001095570.1
Canis lupus familiaris (dog)	PSMB10	XP_546869.3
Mus musculus (house mouse)	Psmb10	NP_038668.2
Homo sapiens (human)	PSMB10	NP_002792.1
Danio rerio (zebrafish)	zgc:92791	NP_001002543.1
Bos taurus (cattle)	PSMB10	NP_001029212.1
Pan troglodytes (chimpanzee)	PSMB10	XP_001166577.2
Rattus norvegicus (Norway rat)	Psmb10	NP_001020808.1
Xenopus tropicalis (tropical clawed frog)	LOC100489016	XP_002941310.2

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

Modified siRNA effectively silence inducible immunoproteasome subunits in NSO cells.
Gvozdeva OV, Belogurov AA Jr, Kuzina ES, Gabibov AG, Meschaninova MI, Ven'yaminova AG, Zenkova MA, Vlassov VV, Chernolovskaya EL
Biochimie12575-82(2016 Jun)

Biological insights from 108 schizophrenia-associated genetic loci.

Nature511(7510)421-7(2014 Jul)

Correlation of LMP10 expression and clinical outcome in Human Papillomavirus (HPV) positive and HPV-Negative tonsillar and base of tongue cancer.
Tertipis N, Haeggblom L, Nordfors C, Gr?n N, N?sman A, Vlastos A, Dalianis T, Ramqvist T
PloS one9(4)e95624(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

designed siRNAs that efficiently silence LMP2, LMP7 and MECL-1 gene expression.
Title: Modified siRNA effectively silence inducible immunoproteasome subunits in NSO cells.

LMP10 nuclear expression in the Human Papillomavirus (HPV)-positive group and LMP10 cytoplasmic expression in the HPV-negative group of patients correlated to better clinical outcome.
Title: Correlation of LMP10 expression and clinical outcome in Human Papillomavirus (HPV) positive and HPV-Negative tonsillar and base of tongue cancer.

Data indicate that treatment-emergent resistance to single-agent bortezomib was independent of variants in the proteasome genes PSMB1, PSMB5, PSMB6, PSMB8, PSMB9, and PSMB10.
Title: Sequence analysis of ?-subunit genes of the 20S proteasome in patients with relapsed multiple myeloma treated with bortezomib or dexamethasone.

Adenovirus E1A causes down-regulation of MECL1 expression
Title: Adenovirus E1A interacts directly with, and regulates the level of expression of, the immunoproteasome component MECL1.

Observational study of gene-disease association, gene-environment interaction, and pharmacogenomic / toxicogenomic. (HuGE Navigator)
Title: Variation at the NFATC2 locus increases the risk of thiazolidinedione-induced edema in the Diabetes REduction Assessment with ramipril and rosiglitazone Medication (DREAM) study.

The following PSMB10 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PSMB10 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu03116	NM_002801.4 Latest version!	Homo sapiens proteasome 20S subunit beta 10 (PSMB10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu03116	NM_002801.3	Homo sapiens proteasome subunit beta 10 (PSMB10), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PSMB10 ( NM_002801.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002792.1 Homo sapiens proteasome 20S subunit beta 10 (PSMB10), mRNA.

Price & Availability↑

CloneID	OHu03116
Clone ID Related Accession (Same CDS sequence)	NM_002801.4 , NM_002801.3
Accession Version	NM_002801.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-29
Organism	Homo sapiens(human)
Product	proteasome subunit beta type-10
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP289065.1 and BM981173.1. On Nov 23, 2018 this sequence version replaced NM_002801.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.276274.1, SRR1163655.467374.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000358514.9/ ENSP00000351314.4 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGCTGAAGC CAGCCCTGGA GCCCCGAGGG GGCTTCTCCT TCGAGAACTG CCAAAGAAAT 
GCATCATTGG AACGCGTCCT CCCGGGGCTC AAGGTCCCTC ACGCACGCAA GACCGGGACC 
ACCATCGCGG GCCTGGTGTT CCAAGACGGG GTCATTCTGG GCGCCGATAC GCGAGCCACT 
AACGATTCGG TCGTGGCGGA CAAGAGCTGC GAGAAGATCC ACTTCATCGC CCCCAAAATC 
TACTGCTGTG GGGCTGGAGT AGCCGCGGAC GCCGAGATGA CCACACGGAT GGTGGCGTCC 
AAGATGGAGC TACACGCGTT ATCTACGGGC CGCGAGCCCC GCGTGGCCAC GGTCACTCGC 
ATCCTGCGCC AGACGCTCTT CAGGTACCAG GGCCACGTGG GTGCATCGCT GATCGTGGGC 
GGCGTAGACC TGACTGGACC GCAGCTCTAC GGTGTGCATC CCCATGGCTC CTACAGCCGT 
CTGCCCTTCA CAGCCCTGGG CTCTGGTCAG GACGCGGCCC TGGCGGTGCT AGAAGACCGG 
TTCCAGCCGA ACATGACGCT GGAGGCTGCT CAGGGGCTGC TGGTGGAAGC CGTCACCGCC 
GGGATCTTGG GTGACCTGGG CTCCGGGGGC AATGTGGACG CATGTGTGAT CACAAAGACT 
GGCGCCAAGC TGCTGCGGAC ACTGAGCTCA CCCACAGAGC CCGTGAAGAG GTCTGGCCGC 
TACCACTTTG TGCCTGGAAC CACAGCTGTC CTGACCCAGA CAGTGAAGCC ACTAACCCTG 
GAGCTAGTGG AGGAAACTGT GCAGGCTATG GAGGTGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002792.1
CDS	102..923
Translation	MLKPALEPRGGFSFENCQRNASLERVLPGLKVPHARKTGTTIAGLVFQDGVILGADTRATNDSVVADKSCEKIHFIAPKIYCCGAGVAADAEMTTRMVASKMELHALSTGREPRVATVTRILRQTLFRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSRLPFTALGSGQDAALAVLEDRFQPNMTLEAAQGLLVEAVTAGILGDLGSGGNVDACVITKTGAKLLRTLSSPTEPVKRSGRYHFVPGTTAVLTQTVKPLTLELVEETVQAMEVE

Target ORF information:

RefSeq Version	NM_002801.4
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 20S subunit beta 10 (PSMB10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002801.4

ORF Insert Sequence:

ATGCTGAAGC CAGCCCTGGA GCCCCGAGGG GGCTTCTCCT TCGAGAACTG CCAAAGAAAT 
GCATCATTGG AACGCGTCCT CCCGGGGCTC AAGGTCCCTC ACGCACGCAA GACCGGGACC 
ACCATCGCGG GCCTGGTGTT CCAAGACGGG GTCATTCTGG GCGCCGATAC GCGAGCCACT 
AACGATTCGG TCGTGGCGGA CAAGAGCTGC GAGAAGATCC ACTTCATCGC CCCCAAAATC 
TACTGCTGTG GGGCTGGAGT AGCCGCGGAC GCCGAGATGA CCACACGGAT GGTGGCGTCC 
AAGATGGAGC TACACGCGTT ATCTACGGGC CGCGAGCCCC GCGTGGCCAC GGTCACTCGC 
ATCCTGCGCC AGACGCTCTT CAGGTACCAG GGCCACGTGG GTGCATCGCT GATCGTGGGC 
GGCGTAGACC TGACTGGACC GCAGCTCTAC GGTGTGCATC CCCATGGCTC CTACAGCCGT 
CTGCCCTTCA CAGCCCTGGG CTCTGGTCAG GACGCGGCCC TGGCGGTGCT AGAAGACCGG 
TTCCAGCCGA ACATGACGCT GGAGGCTGCT CAGGGGCTGC TGGTGGAAGC CGTCACCGCC 
GGGATCTTGG GTGACCTGGG CTCCGGGGGC AATGTGGACG CATGTGTGAT CACAAAGACT 
GGCGCCAAGC TGCTGCGGAC ACTGAGCTCA CCCACAGAGC CCGTGAAGAG GTCTGGCCGC 
TACCACTTTG TGCCTGGAAC CACAGCTGTC CTGACCCAGA CAGTGAAGCC ACTAACCCTG 
GAGCTAGTGG AGGAAACTGT GCAGGCTATG GAGGTGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMB10 ( NM_002801.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002792.1 Homo sapiens proteasome subunit beta 10 (PSMB10), mRNA.

Price & Availability↑

CloneID	OHu03116
Clone ID Related Accession (Same CDS sequence)	NM_002801.4 , NM_002801.3
Accession Version	NM_002801.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 822bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-01
Organism	Homo sapiens(human)
Product	proteasome subunit beta type-10 precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP289065.1 and BM981173.1. On Mar 10, 2011 this sequence version replaced NM_002801.2. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.467374.1, SRR1163655.160151.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2146236, SAMN01820691 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCTGAAGC CAGCCCTGGA GCCCCGAGGG GGCTTCTCCT TCGAGAACTG CCAAAGAAAT 
GCATCATTGG AACGCGTCCT CCCGGGGCTC AAGGTCCCTC ACGCACGCAA GACCGGGACC 
ACCATCGCGG GCCTGGTGTT CCAAGACGGG GTCATTCTGG GCGCCGATAC GCGAGCCACT 
AACGATTCGG TCGTGGCGGA CAAGAGCTGC GAGAAGATCC ACTTCATCGC CCCCAAAATC 
TACTGCTGTG GGGCTGGAGT AGCCGCGGAC GCCGAGATGA CCACACGGAT GGTGGCGTCC 
AAGATGGAGC TACACGCGTT ATCTACGGGC CGCGAGCCCC GCGTGGCCAC GGTCACTCGC 
ATCCTGCGCC AGACGCTCTT CAGGTACCAG GGCCACGTGG GTGCATCGCT GATCGTGGGC 
GGCGTAGACC TGACTGGACC GCAGCTCTAC GGTGTGCATC CCCATGGCTC CTACAGCCGT 
CTGCCCTTCA CAGCCCTGGG CTCTGGTCAG GACGCGGCCC TGGCGGTGCT AGAAGACCGG 
TTCCAGCCGA ACATGACGCT GGAGGCTGCT CAGGGGCTGC TGGTGGAAGC CGTCACCGCC 
GGGATCTTGG GTGACCTGGG CTCCGGGGGC AATGTGGACG CATGTGTGAT CACAAAGACT 
GGCGCCAAGC TGCTGCGGAC ACTGAGCTCA CCCACAGAGC CCGTGAAGAG GTCTGGCCGC 
TACCACTTTG TGCCTGGAAC CACAGCTGTC CTGACCCAGA CAGTGAAGCC ACTAACCCTG 
GAGCTAGTGG AGGAAACTGT GCAGGCTATG GAGGTGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002792.1
CDS	129..950
Translation	MLKPALEPRGGFSFENCQRNASLERVLPGLKVPHARKTGTTIAGLVFQDGVILGADTRATNDSVVADKSCEKIHFIAPKIYCCGAGVAADAEMTTRMVASKMELHALSTGREPRVATVTRILRQTLFRYQGHVGASLIVGGVDLTGPQLYGVHPHGSYSRLPFTALGSGQDAALAVLEDRFQPNMTLEAAQGLLVEAVTAGILGDLGSGGNVDACVITKTGAKLLRTLSSPTEPVKRSGRYHFVPGTTAVLTQTVKPLTLELVEETVQAMEVE

Target ORF information:

RefSeq Version	NM_002801.3
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome subunit beta 10 (PSMB10), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002801.3

ORF Insert Sequence:

ATGCTGAAGC CAGCCCTGGA GCCCCGAGGG GGCTTCTCCT TCGAGAACTG CCAAAGAAAT 
GCATCATTGG AACGCGTCCT CCCGGGGCTC AAGGTCCCTC ACGCACGCAA GACCGGGACC 
ACCATCGCGG GCCTGGTGTT CCAAGACGGG GTCATTCTGG GCGCCGATAC GCGAGCCACT 
AACGATTCGG TCGTGGCGGA CAAGAGCTGC GAGAAGATCC ACTTCATCGC CCCCAAAATC 
TACTGCTGTG GGGCTGGAGT AGCCGCGGAC GCCGAGATGA CCACACGGAT GGTGGCGTCC 
AAGATGGAGC TACACGCGTT ATCTACGGGC CGCGAGCCCC GCGTGGCCAC GGTCACTCGC 
ATCCTGCGCC AGACGCTCTT CAGGTACCAG GGCCACGTGG GTGCATCGCT GATCGTGGGC 
GGCGTAGACC TGACTGGACC GCAGCTCTAC GGTGTGCATC CCCATGGCTC CTACAGCCGT 
CTGCCCTTCA CAGCCCTGGG CTCTGGTCAG GACGCGGCCC TGGCGGTGCT AGAAGACCGG 
TTCCAGCCGA ACATGACGCT GGAGGCTGCT CAGGGGCTGC TGGTGGAAGC CGTCACCGCC 
GGGATCTTGG GTGACCTGGG CTCCGGGGGC AATGTGGACG CATGTGTGAT CACAAAGACT 
GGCGCCAAGC TGCTGCGGAC ACTGAGCTCA CCCACAGAGC CCGTGAAGAG GTCTGGCCGC 
TACCACTTTG TGCCTGGAAC CACAGCTGTC CTGACCCAGA CAGTGAAGCC ACTAACCCTG 
GAGCTAGTGG AGGAAACTGT GCAGGCTATG GAGGTGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

Modified siRNA effectively silence inducible immunoproteasome subunits in NSO cells.
Biochimie12575-82(2016 Jun)
Gvozdeva OV,Belogurov AA Jr,Kuzina ES,Gabibov AG,Meschaninova MI,Ven'yaminova AG,Zenkova MA,Vlassov VV,Chernolovskaya EL

Biological insights from 108 schizophrenia-associated genetic loci.
Nature511(7510)421-7(2014 Jul)

Correlation of LMP10 expression and clinical outcome in Human Papillomavirus (HPV) positive and HPV-Negative tonsillar and base of tongue cancer.
PloS one9(4)e95624(2014)
Tertipis N,Haeggblom L,Nordfors C,Gr?n N,N?sman A,Vlastos A,Dalianis T,Ramqvist T

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PSMB10 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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