PSMB3 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PSMB3
Entrez Gene ID	5691
Full Name	proteasome subunit beta 3
Synonyms	HC10-II
General protein information	Preferred Names proteasome subunit beta 3 Names proteasome subunit beta type-3 proteasome (prosome, macropain) subunit, beta type, 3 proteasome chain 13 proteasome component C10-II proteasome theta chain
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q12
Summary	The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit. The 26 S proteasome may be involved in trinucleotide repeat expansion, a phenomenon which is associated with many hereditary neurological diseases. Pseudogenes have been identified on chromosomes 2 and 12. Alternative splicing results in multiple transcript variants [provided by RefSeq, Sep 2013].
Disorder MIM:	602176

mRNA and Protein(s)

mRNA	Protein	Name
NM_002795.4	NP_002786.2	proteasome subunit beta type-3
NM_002795.3	NP_002786.2	proteasome subunit beta type-3

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Psmb3	NP_036101.1
Rattus norvegicus (Norway rat)	Psmb3	NP_058981.1
Gallus gallus (chicken)	PSMB3	XP_418119.2
Danio rerio (zebrafish)	psmb3	NP_001123295.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP001697	XP_003435908.1
Eremothecium gossypii	AGOS_ABR217C	NP_983166.2
Arabidopsis thaliana (thale cress)	PBC1	NP_564149.1
Xenopus tropicalis (tropical clawed frog)	psmb3	NP_001016503.1
Homo sapiens (human)	PSMB3	NP_002786.2
Neurospora crassa	NCU03304	XP_964481.2
	PSMB3	XP_001084269.1
Canis lupus familiaris (dog)	PSMB3	NP_001239086.1
Arabidopsis thaliana (thale cress)	PBC2	NP_565156.1
Bos taurus (cattle)	PSMB3	NP_001029768.1
Drosophila melanogaster (fruit fly)	Prosbeta3	NP_649858.1
Caenorhabditis elegans (roundworm)	pbs-3	NP_494913.1
Saccharomyces cerevisiae (baker's yeast)	PUP3	NP_011020.3
Kluyveromyces lactis	KLLA0E20197g	XP_454865.1
Schizosaccharomyces pombe (fission yeast)	pup3	NP_587985.1
Magnaporthe oryzae (rice blast fungus)	MGG_04551	XP_003710932.1
Oryza sativa (rice)	Os06g0643100	NP_001058176.1

Gene Ontology
Bibliography

Related articles in PubMed

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

The expression of inflammatory genes is upregulated in peripheral blood of patients with type 1 diabetes.
Jin Y, Sharma A, Carey C, Hopkins D, Wang X, Robertson DG, Bode B, Anderson SW, Reed JC, Steed RD, Steed L, She JX
Diabetes care36(9)2794-802(2013 Sep)

The 26S proteasome drives trinucleotide repeat expansions.
Concannon C, Lahue RS
Nucleic acids research41(12)6098-108(2013 Jul)

Proteomic characterization of the human sperm nucleus.
de Mateo S, Castillo J, Estanyol JM, Ballesc? JL, Oliva R
Proteomics11(13)2714-26(2011 Jul)

AAV exploits subcellular stress associated with inflammation, endoplasmic reticulum expansion, and misfolded proteins in models of cystic fibrosis.
Johnson JS, Gentzsch M, Zhang L, Ribeiro CM, Kantor B, Kafri T, Pickles RJ, Samulski RJ
PLoS pathogens7(5)e1002053(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following PSMB3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PSMB3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu20530	NM_002795.4 Latest version!	Homo sapiens proteasome 20S subunit beta 3 (PSMB3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
View Old Accession Versions >>
OHu20530	NM_002795.3	Homo sapiens proteasome subunit beta 3 (PSMB3), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$49.50-$69.30 $99.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PSMB3 ( NM_002795.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002786.2 Homo sapiens proteasome 20S subunit beta 3 (PSMB3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20530
Clone ID Related Accession (Same CDS sequence)	NM_002795.4 , NM_002795.3
Accession Version	NM_002795.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-09
Organism	Homo sapiens(human)
Product	proteasome subunit beta type-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG942618.1, BC002570.1 and BC013008.2. On Nov 23, 2018 this sequence version replaced NM_002795.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189658.151399.1, SRR5189658.160197.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000619426.5/ ENSP00000483688.1 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGTCTATTA TGTCCTATAA CGGAGGGGCC GTCATGGCCA TGAAGGGGAA GAACTGTGTG 
GCCATCGCTG CAGACAGGCG CTTCGGGATC CAGGCCCAGA TGGTGACCAC GGACTTCCAG 
AAGATCTTTC CCATGGGTGA CCGGCTGTAC ATCGGTCTGG CCGGGCTCGC CACTGACGTC 
CAGACAGTTG CCCAGCGCCT CAAGTTCCGG CTGAACCTGT ATGAGTTGAA GGAAGGTCGG 
CAGATCAAAC CTTATACCCT CATGAGCATG GTGGCCAACC TCTTGTATGA GAAACGGTTT 
GGCCCTTACT ACACTGAGCC AGTCATTGCC GGGTTGGACC CGAAGACCTT TAAGCCCTTC 
ATTTGCTCTC TAGACCTCAT CGGCTGCCCC ATGGTGACTG ATGACTTTGT GGTCAGTGGC 
ACCTGCGCCG AACAAATGTA CGGAATGTGT GAGTCCCTCT GGGAGCCCAA CATGGATCCG 
GATCACCTGT TTGAAACCAT CTCCCAAGCC ATGCTGAATG CTGTGGACCG GGATGCAGTG 
TCAGGCATGG GAGTCATTGT CCACATCATC GAGAAGGACA AAATCACCAC CAGGACACTG 
AAGGCCCGAA TGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002786.2
CDS	87..704
Translation	MSIMSYNGGAVMAMKGKNCVAIAADRRFGIQAQMVTTDFQKIFPMGDRLYIGLAGLATDVQTVAQRLKFRLNLYELKEGRQIKPYTLMSMVANLLYEKRFGPYYTEPVIAGLDPKTFKPFICSLDLIGCPMVTDDFVVSGTCAEQMYGMCESLWEPNMDPDHLFETISQAMLNAVDRDAVSGMGVIVHIIEKDKITTRTLKARMD

Target ORF information:

RefSeq Version	NM_002795.4
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome 20S subunit beta 3 (PSMB3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002795.4

ORF Insert Sequence:

ATGTCTATTA TGTCCTATAA CGGAGGGGCC GTCATGGCCA TGAAGGGGAA GAACTGTGTG 
GCCATCGCTG CAGACAGGCG CTTCGGGATC CAGGCCCAGA TGGTGACCAC GGACTTCCAG 
AAGATCTTTC CCATGGGTGA CCGGCTGTAC ATCGGTCTGG CCGGGCTCGC CACTGACGTC 
CAGACAGTTG CCCAGCGCCT CAAGTTCCGG CTGAACCTGT ATGAGTTGAA GGAAGGTCGG 
CAGATCAAAC CTTATACCCT CATGAGCATG GTGGCCAACC TCTTGTATGA GAAACGGTTT 
GGCCCTTACT ACACTGAGCC AGTCATTGCC GGGTTGGACC CGAAGACCTT TAAGCCCTTC 
ATTTGCTCTC TAGACCTCAT CGGCTGCCCC ATGGTGACTG ATGACTTTGT GGTCAGTGGC 
ACCTGCGCCG AACAAATGTA CGGAATGTGT GAGTCCCTCT GGGAGCCCAA CATGGATCCG 
GATCACCTGT TTGAAACCAT CTCCCAAGCC ATGCTGAATG CTGTGGACCG GGATGCAGTG 
TCAGGCATGG GAGTCATTGT CCACATCATC GAGAAGGACA AAATCACCAC CAGGACACTG 
AAGGCCCGAA TGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PSMB3 ( NM_002795.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002786.2 Homo sapiens proteasome subunit beta 3 (PSMB3), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu20530
Clone ID Related Accession (Same CDS sequence)	NM_002795.4 , NM_002795.3
Accession Version	NM_002795.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 618bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	proteasome subunit beta type-3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC006449.19, BG942618.1, BC002570.1, BC013008.2 and BG184666.1. On Sep 12, 2013 this sequence version replaced NM_002795.2. Transcript Variant: This variant (1) represents the longest transcript and encodes the functional protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.182093.1, SRR1163658.294022.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTCTATTA TGTCCTATAA CGGAGGGGCC GTCATGGCCA TGAAGGGGAA GAACTGTGTG 
GCCATCGCTG CAGACAGGCG CTTCGGGATC CAGGCCCAGA TGGTGACCAC GGACTTCCAG 
AAGATCTTTC CCATGGGTGA CCGGCTGTAC ATCGGTCTGG CCGGGCTCGC CACTGACGTC 
CAGACAGTTG CCCAGCGCCT CAAGTTCCGG CTGAACCTGT ATGAGTTGAA GGAAGGTCGG 
CAGATCAAAC CTTATACCCT CATGAGCATG GTGGCCAACC TCTTGTATGA GAAACGGTTT 
GGCCCTTACT ACACTGAGCC AGTCATTGCC GGGTTGGACC CGAAGACCTT TAAGCCCTTC 
ATTTGCTCTC TAGACCTCAT CGGCTGCCCC ATGGTGACTG ATGACTTTGT GGTCAGTGGC 
ACCTGCGCCG AACAAATGTA CGGAATGTGT GAGTCCCTCT GGGAGCCCAA CATGGATCCG 
GATCACCTGT TTGAAACCAT CTCCCAAGCC ATGCTGAATG CTGTGGACCG GGATGCAGTG 
TCAGGCATGG GAGTCATTGT CCACATCATC GAGAAGGACA AAATCACCAC CAGGACACTG 
AAGGCCCGAA TGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002786.2
CDS	115..732
Translation	MSIMSYNGGAVMAMKGKNCVAIAADRRFGIQAQMVTTDFQKIFPMGDRLYIGLAGLATDVQTVAQRLKFRLNLYELKEGRQIKPYTLMSMVANLLYEKRFGPYYTEPVIAGLDPKTFKPFICSLDLIGCPMVTDDFVVSGTCAEQMYGMCESLWEPNMDPDHLFETISQAMLNAVDRDAVSGMGVIVHIIEKDKITTRTLKARMD

Target ORF information:

RefSeq Version	NM_002795.3
Organism	Homo sapiens(human)
Definition	Homo sapiens proteasome subunit beta 3 (PSMB3), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002795.3

ORF Insert Sequence:

ATGTCTATTA TGTCCTATAA CGGAGGGGCC GTCATGGCCA TGAAGGGGAA GAACTGTGTG 
GCCATCGCTG CAGACAGGCG CTTCGGGATC CAGGCCCAGA TGGTGACCAC GGACTTCCAG 
AAGATCTTTC CCATGGGTGA CCGGCTGTAC ATCGGTCTGG CCGGGCTCGC CACTGACGTC 
CAGACAGTTG CCCAGCGCCT CAAGTTCCGG CTGAACCTGT ATGAGTTGAA GGAAGGTCGG 
CAGATCAAAC CTTATACCCT CATGAGCATG GTGGCCAACC TCTTGTATGA GAAACGGTTT 
GGCCCTTACT ACACTGAGCC AGTCATTGCC GGGTTGGACC CGAAGACCTT TAAGCCCTTC 
ATTTGCTCTC TAGACCTCAT CGGCTGCCCC ATGGTGACTG ATGACTTTGT GGTCAGTGGC 
ACCTGCGCCG AACAAATGTA CGGAATGTGT GAGTCCCTCT GGGAGCCCAA CATGGATCCG 
GATCACCTGT TTGAAACCAT CTCCCAAGCC ATGCTGAATG CTGTGGACCG GGATGCAGTG 
TCAGGCATGG GAGTCATTGT CCACATCATC GAGAAGGACA AAATCACCAC CAGGACACTG 
AAGGCCCGAA TGGACTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

The expression of inflammatory genes is upregulated in peripheral blood of patients with type 1 diabetes.
Diabetes care36(9)2794-802(2013 Sep)
Jin Y,Sharma A,Carey C,Hopkins D,Wang X,Robertson DG,Bode B,Anderson SW,Reed JC,Steed RD,Steed L,She JX

The 26S proteasome drives trinucleotide repeat expansions.
Nucleic acids research41(12)6098-108(2013 Jul)
Concannon C,Lahue RS

Proteomic characterization of the human sperm nucleus.
Proteomics11(13)2714-26(2011 Jul)
de Mateo S,Castillo J,Estanyol JM,Ballesc? JL,Oliva R

AAV exploits subcellular stress associated with inflammation, endoplasmic reticulum expansion, and misfolded proteins in models of cystic fibrosis.
PLoS pathogens7(5)e1002053(2011 May)
Johnson JS,Gentzsch M,Zhang L,Ribeiro CM,Kantor B,Kafri T,Pickles RJ,Samulski RJ

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PSMB3 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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