SPHK2 cDNA ORF clone, Homo sapiens(human)

The following SPHK2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPHK2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu16897 NM_001204160.3
Latest version!
Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu16910 NM_001204159.3
Latest version!
Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu16910 NM_020126.5
Latest version!
Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu16897 XM_006723292.1
Latest version!
Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu16897 XM_011527134.1
Latest version!
Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu16910 XM_006723291.1
Latest version!
Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu16910 XM_011527133.1
Latest version!
Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu16837 NM_001243876.1
Latest version!
Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OHu16837 XM_017027009.1
Latest version!
Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $223.30
$319.00
OHu16817 NM_001204158.3
Latest version!
Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu70093 XM_017027010.2
Latest version!
Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu88577 XM_017027008.1
Latest version!
Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
View Old Accession Versions >>
OHu70093 XM_017027010.1 Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $223.30
$319.00
OHu16897 NM_001204160.2 Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu16910 NM_020126.4 Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu16910 NM_001204159.2 Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu16817 NM_001204158.2 Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu16897
    Clone ID Related Accession (Same CDS sequence) XM_006723292.1 , NM_001204160.3 , XM_011527134.1 , NM_001204160.2
    Accession Version NM_001204160.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC010671.1. On May 31, 2019 this sequence version replaced NM_001204160.2. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010671.1, SRR7410570.656689.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCGC CCCCACCGCC ACTGGCTGCC AGCACCCCGC TCCTCCATGG CGAGTTTGGC 
    TCCTACCCAG CCCGAGGCCC ACGCTTTGCC CTCACCCTTA CATCGCAGGC CCTGCACATA
    CAGCGGCTGC GCCCCAAACC TGAAGCCAGG CCCCGGGGTG GCCTGGTCCC GTTGGCCGAG
    GTCTCAGGCT GCTGCACCCT GCGAAGCCGC AGCCCCTCAG ACTCAGCGGC CTACTTCTGC
    ATCTACACCT ACCCTCGGGG CCGGCGCGGG GCCCGGCGCA GAGCCACTCG CACCTTCCGG
    GCAGATGGGG CCGCCACCTA CGAAGAGAAC CGTGCCGAGG CCCAGCGCTG GGCCACTGCC
    CTCACCTGTC TGCTCCGAGG ACTGCCACTG CCCGGGGATG GGGAGATCAC CCCTGACCTG
    CTACCTCGGC CGCCCCGGTT GCTTCTATTG GTCAATCCCT TTGGGGGTCG GGGCCTGGCC
    TGGCAGTGGT GTAAGAACCA CGTGCTTCCC ATGATCTCTG AAGCTGGGCT GTCCTTCAAC
    CTCATCCAGA CAGAACGACA GAACCACGCC CGGGAGCTGG TCCAGGGGCT GAGCCTGAGT
    GAGTGGGATG GCATCGTCAC GGTCTCGGGA GACGGGCTGC TCCATGAGGT GCTGAACGGG
    CTCCTAGATC GCCCTGACTG GGAGGAAGCT GTGAAGATGC CTGTGGGCAT CCTCCCCTGC
    GGCTCGGGCA ACGCGCTGGC CGGAGCAGTG AACCAGCACG GGGGATTTGA GCCAGCCCTG
    GGCCTCGACC TGTTGCTCAA CTGCTCACTG TTGCTGTGCC GGGGTGGTGG CCACCCACTG
    GACCTGCTCT CCGTGACGCT GGCCTCGGGC TCCCGCTGTT TCTCCTTCCT GTCTGTGGCC
    TGGGGCTTCG TGTCAGATGT GGATATCCAG AGCGAGCGCT TCAGGGCCTT GGGCAGTGCC
    CGCTTCACAC TGGGCACGGT GCTGGGCCTC GCCACACTGC ACACCTACCG CGGACGCCTC
    TCCTACCTCC CCGCCACTGT GGAACCTGCC TCGCCCACCC CTGCCCATAG CCTGCCTCGT
    GCCAAGTCGG AGCTGACCCT AACCCCAGAC CCAGCCCCGC CCATGGCCCA CTCACCCCTG
    CATCGTTCTG TGTCTGACCT GCCTCTTCCC CTGCCCCAGC CTGCCCTGGC CTCTCCTGGC
    TCGCCAGAAC CCCTGCCCAT CCTGTCCCTC AACGGTGGGG GCCCAGAGCT GGCTGGGGAC
    TGGGGTGGGG CTGGGGATGC TCCGCTGTCC CCGGACCCAC TGCTGTCTTC ACCTCCTGGC
    TCTCCCAAGG CAGCTCTACA CTCACCCGTC TCCGAAGGGG CCCCCGTAAT TCCCCCATCC
    TCTGGGCTCC CACTTCCCAC CCCTGATGCC CGGGTAGGGG CCTCCACCTG CGGCCCGCCC
    GACCACCTGC TGCCTCCGCT GGGCACCCCG CTGCCCCCAG ACTGGGTGAC GCTGGAGGGG
    GACTTTGTGC TCATGTTGGC CATCTCGCCC AGCCACCTAG GCGCTGACCT GGTGGCAGCT
    CCGCATGCGC GCTTCGACGA CGGCCTGGTG CACCTGTGCT GGGTGCGTAG CGGCATCTCG
    CGGGCTGCGC TGCTGCGCCT TTTCTTGGCC ATGGAGCGTG GTAGCCACTT CAGCCTGGGC
    TGTCCGCAGC TGGGCTACGC CGCGGCCCGT GCCTTCCGCC TAGAGCCGCT CACACCACGC
    GGCGTGCTCA CAGTGGACGG GGAGCAGGTG GAGTATGGGC CGCTACAGGC ACAGATGCAC
    CCTGGCATCG GTACACTGCT CACTGGGCCT CCTGGCTGCC CGGGGCGGGA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191089.1
    CDS118..1974
    Translation

    Target ORF information:

    RefSeq Version NM_001204160.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204160.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCGC CCCCACCGCC ACTGGCTGCC AGCACCCCGC TCCTCCATGG CGAGTTTGGC 
    TCCTACCCAG CCCGAGGCCC ACGCTTTGCC CTCACCCTTA CATCGCAGGC CCTGCACATA
    CAGCGGCTGC GCCCCAAACC TGAAGCCAGG CCCCGGGGTG GCCTGGTCCC GTTGGCCGAG
    GTCTCAGGCT GCTGCACCCT GCGAAGCCGC AGCCCCTCAG ACTCAGCGGC CTACTTCTGC
    ATCTACACCT ACCCTCGGGG CCGGCGCGGG GCCCGGCGCA GAGCCACTCG CACCTTCCGG
    GCAGATGGGG CCGCCACCTA CGAAGAGAAC CGTGCCGAGG CCCAGCGCTG GGCCACTGCC
    CTCACCTGTC TGCTCCGAGG ACTGCCACTG CCCGGGGATG GGGAGATCAC CCCTGACCTG
    CTACCTCGGC CGCCCCGGTT GCTTCTATTG GTCAATCCCT TTGGGGGTCG GGGCCTGGCC
    TGGCAGTGGT GTAAGAACCA CGTGCTTCCC ATGATCTCTG AAGCTGGGCT GTCCTTCAAC
    CTCATCCAGA CAGAACGACA GAACCACGCC CGGGAGCTGG TCCAGGGGCT GAGCCTGAGT
    GAGTGGGATG GCATCGTCAC GGTCTCGGGA GACGGGCTGC TCCATGAGGT GCTGAACGGG
    CTCCTAGATC GCCCTGACTG GGAGGAAGCT GTGAAGATGC CTGTGGGCAT CCTCCCCTGC
    GGCTCGGGCA ACGCGCTGGC CGGAGCAGTG AACCAGCACG GGGGATTTGA GCCAGCCCTG
    GGCCTCGACC TGTTGCTCAA CTGCTCACTG TTGCTGTGCC GGGGTGGTGG CCACCCACTG
    GACCTGCTCT CCGTGACGCT GGCCTCGGGC TCCCGCTGTT TCTCCTTCCT GTCTGTGGCC
    TGGGGCTTCG TGTCAGATGT GGATATCCAG AGCGAGCGCT TCAGGGCCTT GGGCAGTGCC
    CGCTTCACAC TGGGCACGGT GCTGGGCCTC GCCACACTGC ACACCTACCG CGGACGCCTC
    TCCTACCTCC CCGCCACTGT GGAACCTGCC TCGCCCACCC CTGCCCATAG CCTGCCTCGT
    GCCAAGTCGG AGCTGACCCT AACCCCAGAC CCAGCCCCGC CCATGGCCCA CTCACCCCTG
    CATCGTTCTG TGTCTGACCT GCCTCTTCCC CTGCCCCAGC CTGCCCTGGC CTCTCCTGGC
    TCGCCAGAAC CCCTGCCCAT CCTGTCCCTC AACGGTGGGG GCCCAGAGCT GGCTGGGGAC
    TGGGGTGGGG CTGGGGATGC TCCGCTGTCC CCGGACCCAC TGCTGTCTTC ACCTCCTGGC
    TCTCCCAAGG CAGCTCTACA CTCACCCGTC TCCGAAGGGG CCCCCGTAAT TCCCCCATCC
    TCTGGGCTCC CACTTCCCAC CCCTGATGCC CGGGTAGGGG CCTCCACCTG CGGCCCGCCC
    GACCACCTGC TGCCTCCGCT GGGCACCCCG CTGCCCCCAG ACTGGGTGAC GCTGGAGGGG
    GACTTTGTGC TCATGTTGGC CATCTCGCCC AGCCACCTAG GCGCTGACCT GGTGGCAGCT
    CCGCATGCGC GCTTCGACGA CGGCCTGGTG CACCTGTGCT GGGTGCGTAG CGGCATCTCG
    CGGGCTGCGC TGCTGCGCCT TTTCTTGGCC ATGGAGCGTG GTAGCCACTT CAGCCTGGGC
    TGTCCGCAGC TGGGCTACGC CGCGGCCCGT GCCTTCCGCC TAGAGCCGCT CACACCACGC
    GGCGTGCTCA CAGTGGACGG GGAGCAGGTG GAGTATGGGC CGCTACAGGC ACAGATGCAC
    CCTGGCATCG GTACACTGCT CACTGGGCCT CCTGGCTGCC CGGGGCGGGA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16897
    Clone ID Related Accession (Same CDS sequence) XM_006723292.1 , NM_001204160.3 , XM_011527134.1 , NM_001204160.2
    Accession Version NM_001204160.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC010671.1 and DB367710.1. On Sep 1, 2011 this sequence version replaced NM_001204160.1. Transcript Variant: This variant (4) differs in the 5' UTR, lacks a portion of the 5' coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (c) has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC010671.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCGC CCCCACCGCC ACTGGCTGCC AGCACCCCGC TCCTCCATGG CGAGTTTGGC 
    TCCTACCCAG CCCGAGGCCC ACGCTTTGCC CTCACCCTTA CATCGCAGGC CCTGCACATA
    CAGCGGCTGC GCCCCAAACC TGAAGCCAGG CCCCGGGGTG GCCTGGTCCC GTTGGCCGAG
    GTCTCAGGCT GCTGCACCCT GCGAAGCCGC AGCCCCTCAG ACTCAGCGGC CTACTTCTGC
    ATCTACACCT ACCCTCGGGG CCGGCGCGGG GCCCGGCGCA GAGCCACTCG CACCTTCCGG
    GCAGATGGGG CCGCCACCTA CGAAGAGAAC CGTGCCGAGG CCCAGCGCTG GGCCACTGCC
    CTCACCTGTC TGCTCCGAGG ACTGCCACTG CCCGGGGATG GGGAGATCAC CCCTGACCTG
    CTACCTCGGC CGCCCCGGTT GCTTCTATTG GTCAATCCCT TTGGGGGTCG GGGCCTGGCC
    TGGCAGTGGT GTAAGAACCA CGTGCTTCCC ATGATCTCTG AAGCTGGGCT GTCCTTCAAC
    CTCATCCAGA CAGAACGACA GAACCACGCC CGGGAGCTGG TCCAGGGGCT GAGCCTGAGT
    GAGTGGGATG GCATCGTCAC GGTCTCGGGA GACGGGCTGC TCCATGAGGT GCTGAACGGG
    CTCCTAGATC GCCCTGACTG GGAGGAAGCT GTGAAGATGC CTGTGGGCAT CCTCCCCTGC
    GGCTCGGGCA ACGCGCTGGC CGGAGCAGTG AACCAGCACG GGGGATTTGA GCCAGCCCTG
    GGCCTCGACC TGTTGCTCAA CTGCTCACTG TTGCTGTGCC GGGGTGGTGG CCACCCACTG
    GACCTGCTCT CCGTGACGCT GGCCTCGGGC TCCCGCTGTT TCTCCTTCCT GTCTGTGGCC
    TGGGGCTTCG TGTCAGATGT GGATATCCAG AGCGAGCGCT TCAGGGCCTT GGGCAGTGCC
    CGCTTCACAC TGGGCACGGT GCTGGGCCTC GCCACACTGC ACACCTACCG CGGACGCCTC
    TCCTACCTCC CCGCCACTGT GGAACCTGCC TCGCCCACCC CTGCCCATAG CCTGCCTCGT
    GCCAAGTCGG AGCTGACCCT AACCCCAGAC CCAGCCCCGC CCATGGCCCA CTCACCCCTG
    CATCGTTCTG TGTCTGACCT GCCTCTTCCC CTGCCCCAGC CTGCCCTGGC CTCTCCTGGC
    TCGCCAGAAC CCCTGCCCAT CCTGTCCCTC AACGGTGGGG GCCCAGAGCT GGCTGGGGAC
    TGGGGTGGGG CTGGGGATGC TCCGCTGTCC CCGGACCCAC TGCTGTCTTC ACCTCCTGGC
    TCTCCCAAGG CAGCTCTACA CTCACCCGTC TCCGAAGGGG CCCCCGTAAT TCCCCCATCC
    TCTGGGCTCC CACTTCCCAC CCCTGATGCC CGGGTAGGGG CCTCCACCTG CGGCCCGCCC
    GACCACCTGC TGCCTCCGCT GGGCACCCCG CTGCCCCCAG ACTGGGTGAC GCTGGAGGGG
    GACTTTGTGC TCATGTTGGC CATCTCGCCC AGCCACCTAG GCGCTGACCT GGTGGCAGCT
    CCGCATGCGC GCTTCGACGA CGGCCTGGTG CACCTGTGCT GGGTGCGTAG CGGCATCTCG
    CGGGCTGCGC TGCTGCGCCT TTTCTTGGCC ATGGAGCGTG GTAGCCACTT CAGCCTGGGC
    TGTCCGCAGC TGGGCTACGC CGCGGCCCGT GCCTTCCGCC TAGAGCCGCT CACACCACGC
    GGCGTGCTCA CAGTGGACGG GGAGCAGGTG GAGTATGGGC CGCTACAGGC ACAGATGCAC
    CCTGGCATCG GTACACTGCT CACTGGGCCT CCTGGCTGCC CGGGGCGGGA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191089.1
    CDS195..2051
    Translation

    Target ORF information:

    RefSeq Version NM_001204160.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204160.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCGC CCCCACCGCC ACTGGCTGCC AGCACCCCGC TCCTCCATGG CGAGTTTGGC 
    TCCTACCCAG CCCGAGGCCC ACGCTTTGCC CTCACCCTTA CATCGCAGGC CCTGCACATA
    CAGCGGCTGC GCCCCAAACC TGAAGCCAGG CCCCGGGGTG GCCTGGTCCC GTTGGCCGAG
    GTCTCAGGCT GCTGCACCCT GCGAAGCCGC AGCCCCTCAG ACTCAGCGGC CTACTTCTGC
    ATCTACACCT ACCCTCGGGG CCGGCGCGGG GCCCGGCGCA GAGCCACTCG CACCTTCCGG
    GCAGATGGGG CCGCCACCTA CGAAGAGAAC CGTGCCGAGG CCCAGCGCTG GGCCACTGCC
    CTCACCTGTC TGCTCCGAGG ACTGCCACTG CCCGGGGATG GGGAGATCAC CCCTGACCTG
    CTACCTCGGC CGCCCCGGTT GCTTCTATTG GTCAATCCCT TTGGGGGTCG GGGCCTGGCC
    TGGCAGTGGT GTAAGAACCA CGTGCTTCCC ATGATCTCTG AAGCTGGGCT GTCCTTCAAC
    CTCATCCAGA CAGAACGACA GAACCACGCC CGGGAGCTGG TCCAGGGGCT GAGCCTGAGT
    GAGTGGGATG GCATCGTCAC GGTCTCGGGA GACGGGCTGC TCCATGAGGT GCTGAACGGG
    CTCCTAGATC GCCCTGACTG GGAGGAAGCT GTGAAGATGC CTGTGGGCAT CCTCCCCTGC
    GGCTCGGGCA ACGCGCTGGC CGGAGCAGTG AACCAGCACG GGGGATTTGA GCCAGCCCTG
    GGCCTCGACC TGTTGCTCAA CTGCTCACTG TTGCTGTGCC GGGGTGGTGG CCACCCACTG
    GACCTGCTCT CCGTGACGCT GGCCTCGGGC TCCCGCTGTT TCTCCTTCCT GTCTGTGGCC
    TGGGGCTTCG TGTCAGATGT GGATATCCAG AGCGAGCGCT TCAGGGCCTT GGGCAGTGCC
    CGCTTCACAC TGGGCACGGT GCTGGGCCTC GCCACACTGC ACACCTACCG CGGACGCCTC
    TCCTACCTCC CCGCCACTGT GGAACCTGCC TCGCCCACCC CTGCCCATAG CCTGCCTCGT
    GCCAAGTCGG AGCTGACCCT AACCCCAGAC CCAGCCCCGC CCATGGCCCA CTCACCCCTG
    CATCGTTCTG TGTCTGACCT GCCTCTTCCC CTGCCCCAGC CTGCCCTGGC CTCTCCTGGC
    TCGCCAGAAC CCCTGCCCAT CCTGTCCCTC AACGGTGGGG GCCCAGAGCT GGCTGGGGAC
    TGGGGTGGGG CTGGGGATGC TCCGCTGTCC CCGGACCCAC TGCTGTCTTC ACCTCCTGGC
    TCTCCCAAGG CAGCTCTACA CTCACCCGTC TCCGAAGGGG CCCCCGTAAT TCCCCCATCC
    TCTGGGCTCC CACTTCCCAC CCCTGATGCC CGGGTAGGGG CCTCCACCTG CGGCCCGCCC
    GACCACCTGC TGCCTCCGCT GGGCACCCCG CTGCCCCCAG ACTGGGTGAC GCTGGAGGGG
    GACTTTGTGC TCATGTTGGC CATCTCGCCC AGCCACCTAG GCGCTGACCT GGTGGCAGCT
    CCGCATGCGC GCTTCGACGA CGGCCTGGTG CACCTGTGCT GGGTGCGTAG CGGCATCTCG
    CGGGCTGCGC TGCTGCGCCT TTTCTTGGCC ATGGAGCGTG GTAGCCACTT CAGCCTGGGC
    TGTCCGCAGC TGGGCTACGC CGCGGCCCGT GCCTTCCGCC TAGAGCCGCT CACACCACGC
    GGCGTGCTCA CAGTGGACGG GGAGCAGGTG GAGTATGGGC CGCTACAGGC ACAGATGCAC
    CCTGGCATCG GTACACTGCT CACTGGGCCT CCTGGCTGCC CGGGGCGGGA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16910
    Clone ID Related Accession (Same CDS sequence) NM_001204159.2 , XM_006723291.1 , NM_020126.5 , NM_020126.4 , NM_001204159.3 , XM_011527133.1
    Accession Version NM_001204159.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB053266.1, AL136701.1, AF245447.1 and DB367710.1. On Sep 1, 2011 this sequence version replaced NM_001204159.1. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1 and 3 both encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL136701.1, SRR1803617.350859.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGATTTGAGC CAGCCCTGGG CCTCGACCTG
    TTGCTCAACT GCTCACTGTT GCTGTGCCGG GGTGGTGGCC ACCCACTGGA CCTGCTCTCC
    GTGACGCTGG CCTCGGGCTC CCGCTGTTTC TCCTTCCTGT CTGTGGCCTG GGGCTTCGTG
    TCAGATGTGG ATATCCAGAG CGAGCGCTTC AGGGCCTTGG GCAGTGCCCG CTTCACACTG
    GGCACGGTGC TGGGCCTCGC CACACTGCAC ACCTACCGCG GACGCCTCTC CTACCTCCCC
    GCCACTGTGG AACCTGCCTC GCCCACCCCT GCCCATAGCC TGCCTCGTGC CAAGTCGGAG
    CTGACCCTAA CCCCAGACCC AGCCCCGCCC ATGGCCCACT CACCCCTGCA TCGTTCTGTG
    TCTGACCTGC CTCTTCCCCT GCCCCAGCCT GCCCTGGCCT CTCCTGGCTC GCCAGAACCC
    CTGCCCATCC TGTCCCTCAA CGGTGGGGGC CCAGAGCTGG CTGGGGACTG GGGTGGGGCT
    GGGGATGCTC CGCTGTCCCC GGACCCACTG CTGTCTTCAC CTCCTGGCTC TCCCAAGGCA
    GCTCTACACT CACCCGTCTC CGAAGGGGCC CCCGTAATTC CCCCATCCTC TGGGCTCCCA
    CTTCCCACCC CTGATGCCCG GGTAGGGGCC TCCACCTGCG GCCCGCCCGA CCACCTGCTG
    CCTCCGCTGG GCACCCCGCT GCCCCCAGAC TGGGTGACGC TGGAGGGGGA CTTTGTGCTC
    ATGTTGGCCA TCTCGCCCAG CCACCTAGGC GCTGACCTGG TGGCAGCTCC GCATGCGCGC
    TTCGACGACG GCCTGGTGCA CCTGTGCTGG GTGCGTAGCG GCATCTCGCG GGCTGCGCTG
    CTGCGCCTTT TCTTGGCCAT GGAGCGTGGT AGCCACTTCA GCCTGGGCTG TCCGCAGCTG
    GGCTACGCCG CGGCCCGTGC CTTCCGCCTA GAGCCGCTCA CACCACGCGG CGTGCTCACA
    GTGGACGGGG AGCAGGTGGA GTATGGGCCG CTACAGGCAC AGATGCACCC TGGCATCGGT
    ACACTGCTCA CTGGGCCTCC TGGCTGCCCG GGGCGGGAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191088.1
    CDS271..2235
    Translation

    Target ORF information:

    RefSeq Version NM_001204159.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204159.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGATTTGAGC CAGCCCTGGG CCTCGACCTG
    TTGCTCAACT GCTCACTGTT GCTGTGCCGG GGTGGTGGCC ACCCACTGGA CCTGCTCTCC
    GTGACGCTGG CCTCGGGCTC CCGCTGTTTC TCCTTCCTGT CTGTGGCCTG GGGCTTCGTG
    TCAGATGTGG ATATCCAGAG CGAGCGCTTC AGGGCCTTGG GCAGTGCCCG CTTCACACTG
    GGCACGGTGC TGGGCCTCGC CACACTGCAC ACCTACCGCG GACGCCTCTC CTACCTCCCC
    GCCACTGTGG AACCTGCCTC GCCCACCCCT GCCCATAGCC TGCCTCGTGC CAAGTCGGAG
    CTGACCCTAA CCCCAGACCC AGCCCCGCCC ATGGCCCACT CACCCCTGCA TCGTTCTGTG
    TCTGACCTGC CTCTTCCCCT GCCCCAGCCT GCCCTGGCCT CTCCTGGCTC GCCAGAACCC
    CTGCCCATCC TGTCCCTCAA CGGTGGGGGC CCAGAGCTGG CTGGGGACTG GGGTGGGGCT
    GGGGATGCTC CGCTGTCCCC GGACCCACTG CTGTCTTCAC CTCCTGGCTC TCCCAAGGCA
    GCTCTACACT CACCCGTCTC CGAAGGGGCC CCCGTAATTC CCCCATCCTC TGGGCTCCCA
    CTTCCCACCC CTGATGCCCG GGTAGGGGCC TCCACCTGCG GCCCGCCCGA CCACCTGCTG
    CCTCCGCTGG GCACCCCGCT GCCCCCAGAC TGGGTGACGC TGGAGGGGGA CTTTGTGCTC
    ATGTTGGCCA TCTCGCCCAG CCACCTAGGC GCTGACCTGG TGGCAGCTCC GCATGCGCGC
    TTCGACGACG GCCTGGTGCA CCTGTGCTGG GTGCGTAGCG GCATCTCGCG GGCTGCGCTG
    CTGCGCCTTT TCTTGGCCAT GGAGCGTGGT AGCCACTTCA GCCTGGGCTG TCCGCAGCTG
    GGCTACGCCG CGGCCCGTGC CTTCCGCCTA GAGCCGCTCA CACCACGCGG CGTGCTCACA
    GTGGACGGGG AGCAGGTGGA GTATGGGCCG CTACAGGCAC AGATGCACCC TGGCATCGGT
    ACACTGCTCA CTGGGCCTCC TGGCTGCCCG GGGCGGGAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16910
    Clone ID Related Accession (Same CDS sequence) NM_001204159.2 , XM_006723291.1 , NM_020126.5 , NM_020126.4 , NM_001204159.3 , XM_011527133.1
    Accession Version NM_020126.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-01
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC006161.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_020126.4. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 3 both encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR3476690.1085117.1, BC006161.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000245222.9/ ENSP00000245222.3 RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGATTTGAGC CAGCCCTGGG CCTCGACCTG
    TTGCTCAACT GCTCACTGTT GCTGTGCCGG GGTGGTGGCC ACCCACTGGA CCTGCTCTCC
    GTGACGCTGG CCTCGGGCTC CCGCTGTTTC TCCTTCCTGT CTGTGGCCTG GGGCTTCGTG
    TCAGATGTGG ATATCCAGAG CGAGCGCTTC AGGGCCTTGG GCAGTGCCCG CTTCACACTG
    GGCACGGTGC TGGGCCTCGC CACACTGCAC ACCTACCGCG GACGCCTCTC CTACCTCCCC
    GCCACTGTGG AACCTGCCTC GCCCACCCCT GCCCATAGCC TGCCTCGTGC CAAGTCGGAG
    CTGACCCTAA CCCCAGACCC AGCCCCGCCC ATGGCCCACT CACCCCTGCA TCGTTCTGTG
    TCTGACCTGC CTCTTCCCCT GCCCCAGCCT GCCCTGGCCT CTCCTGGCTC GCCAGAACCC
    CTGCCCATCC TGTCCCTCAA CGGTGGGGGC CCAGAGCTGG CTGGGGACTG GGGTGGGGCT
    GGGGATGCTC CGCTGTCCCC GGACCCACTG CTGTCTTCAC CTCCTGGCTC TCCCAAGGCA
    GCTCTACACT CACCCGTCTC CGAAGGGGCC CCCGTAATTC CCCCATCCTC TGGGCTCCCA
    CTTCCCACCC CTGATGCCCG GGTAGGGGCC TCCACCTGCG GCCCGCCCGA CCACCTGCTG
    CCTCCGCTGG GCACCCCGCT GCCCCCAGAC TGGGTGACGC TGGAGGGGGA CTTTGTGCTC
    ATGTTGGCCA TCTCGCCCAG CCACCTAGGC GCTGACCTGG TGGCAGCTCC GCATGCGCGC
    TTCGACGACG GCCTGGTGCA CCTGTGCTGG GTGCGTAGCG GCATCTCGCG GGCTGCGCTG
    CTGCGCCTTT TCTTGGCCAT GGAGCGTGGT AGCCACTTCA GCCTGGGCTG TCCGCAGCTG
    GGCTACGCCG CGGCCCGTGC CTTCCGCCTA GAGCCGCTCA CACCACGCGG CGTGCTCACA
    GTGGACGGGG AGCAGGTGGA GTATGGGCCG CTACAGGCAC AGATGCACCC TGGCATCGGT
    ACACTGCTCA CTGGGCCTCC TGGCTGCCCG GGGCGGGAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_064511.2
    CDS152..2116
    Translation

    Target ORF information:

    RefSeq Version NM_020126.5
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020126.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGATTTGAGC CAGCCCTGGG CCTCGACCTG
    TTGCTCAACT GCTCACTGTT GCTGTGCCGG GGTGGTGGCC ACCCACTGGA CCTGCTCTCC
    GTGACGCTGG CCTCGGGCTC CCGCTGTTTC TCCTTCCTGT CTGTGGCCTG GGGCTTCGTG
    TCAGATGTGG ATATCCAGAG CGAGCGCTTC AGGGCCTTGG GCAGTGCCCG CTTCACACTG
    GGCACGGTGC TGGGCCTCGC CACACTGCAC ACCTACCGCG GACGCCTCTC CTACCTCCCC
    GCCACTGTGG AACCTGCCTC GCCCACCCCT GCCCATAGCC TGCCTCGTGC CAAGTCGGAG
    CTGACCCTAA CCCCAGACCC AGCCCCGCCC ATGGCCCACT CACCCCTGCA TCGTTCTGTG
    TCTGACCTGC CTCTTCCCCT GCCCCAGCCT GCCCTGGCCT CTCCTGGCTC GCCAGAACCC
    CTGCCCATCC TGTCCCTCAA CGGTGGGGGC CCAGAGCTGG CTGGGGACTG GGGTGGGGCT
    GGGGATGCTC CGCTGTCCCC GGACCCACTG CTGTCTTCAC CTCCTGGCTC TCCCAAGGCA
    GCTCTACACT CACCCGTCTC CGAAGGGGCC CCCGTAATTC CCCCATCCTC TGGGCTCCCA
    CTTCCCACCC CTGATGCCCG GGTAGGGGCC TCCACCTGCG GCCCGCCCGA CCACCTGCTG
    CCTCCGCTGG GCACCCCGCT GCCCCCAGAC TGGGTGACGC TGGAGGGGGA CTTTGTGCTC
    ATGTTGGCCA TCTCGCCCAG CCACCTAGGC GCTGACCTGG TGGCAGCTCC GCATGCGCGC
    TTCGACGACG GCCTGGTGCA CCTGTGCTGG GTGCGTAGCG GCATCTCGCG GGCTGCGCTG
    CTGCGCCTTT TCTTGGCCAT GGAGCGTGGT AGCCACTTCA GCCTGGGCTG TCCGCAGCTG
    GGCTACGCCG CGGCCCGTGC CTTCCGCCTA GAGCCGCTCA CACCACGCGG CGTGCTCACA
    GTGGACGGGG AGCAGGTGGA GTATGGGCCG CTACAGGCAC AGATGCACCC TGGCATCGGT
    ACACTGCTCA CTGGGCCTCC TGGCTGCCCG GGGCGGGAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16910
    Clone ID Related Accession (Same CDS sequence) NM_001204159.2 , XM_006723291.1 , NM_020126.5 , NM_020126.4 , NM_001204159.3 , XM_011527133.1
    Accession Version NM_020126.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BC006161.1 and DB367710.1. This sequence is a reference standard in the RefSeqGene project. On Sep 1, 2011 this sequence version replaced NM_020126.3. Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 3 both encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC006161.1, SRR1660805.395595.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGATTTGAGC CAGCCCTGGG CCTCGACCTG
    TTGCTCAACT GCTCACTGTT GCTGTGCCGG GGTGGTGGCC ACCCACTGGA CCTGCTCTCC
    GTGACGCTGG CCTCGGGCTC CCGCTGTTTC TCCTTCCTGT CTGTGGCCTG GGGCTTCGTG
    TCAGATGTGG ATATCCAGAG CGAGCGCTTC AGGGCCTTGG GCAGTGCCCG CTTCACACTG
    GGCACGGTGC TGGGCCTCGC CACACTGCAC ACCTACCGCG GACGCCTCTC CTACCTCCCC
    GCCACTGTGG AACCTGCCTC GCCCACCCCT GCCCATAGCC TGCCTCGTGC CAAGTCGGAG
    CTGACCCTAA CCCCAGACCC AGCCCCGCCC ATGGCCCACT CACCCCTGCA TCGTTCTGTG
    TCTGACCTGC CTCTTCCCCT GCCCCAGCCT GCCCTGGCCT CTCCTGGCTC GCCAGAACCC
    CTGCCCATCC TGTCCCTCAA CGGTGGGGGC CCAGAGCTGG CTGGGGACTG GGGTGGGGCT
    GGGGATGCTC CGCTGTCCCC GGACCCACTG CTGTCTTCAC CTCCTGGCTC TCCCAAGGCA
    GCTCTACACT CACCCGTCTC CGAAGGGGCC CCCGTAATTC CCCCATCCTC TGGGCTCCCA
    CTTCCCACCC CTGATGCCCG GGTAGGGGCC TCCACCTGCG GCCCGCCCGA CCACCTGCTG
    CCTCCGCTGG GCACCCCGCT GCCCCCAGAC TGGGTGACGC TGGAGGGGGA CTTTGTGCTC
    ATGTTGGCCA TCTCGCCCAG CCACCTAGGC GCTGACCTGG TGGCAGCTCC GCATGCGCGC
    TTCGACGACG GCCTGGTGCA CCTGTGCTGG GTGCGTAGCG GCATCTCGCG GGCTGCGCTG
    CTGCGCCTTT TCTTGGCCAT GGAGCGTGGT AGCCACTTCA GCCTGGGCTG TCCGCAGCTG
    GGCTACGCCG CGGCCCGTGC CTTCCGCCTA GAGCCGCTCA CACCACGCGG CGTGCTCACA
    GTGGACGGGG AGCAGGTGGA GTATGGGCCG CTACAGGCAC AGATGCACCC TGGCATCGGT
    ACACTGCTCA CTGGGCCTCC TGGCTGCCCG GGGCGGGAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_064511.2
    CDS367..2331
    Translation

    Target ORF information:

    RefSeq Version NM_020126.4
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_020126.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGATTTGAGC CAGCCCTGGG CCTCGACCTG
    TTGCTCAACT GCTCACTGTT GCTGTGCCGG GGTGGTGGCC ACCCACTGGA CCTGCTCTCC
    GTGACGCTGG CCTCGGGCTC CCGCTGTTTC TCCTTCCTGT CTGTGGCCTG GGGCTTCGTG
    TCAGATGTGG ATATCCAGAG CGAGCGCTTC AGGGCCTTGG GCAGTGCCCG CTTCACACTG
    GGCACGGTGC TGGGCCTCGC CACACTGCAC ACCTACCGCG GACGCCTCTC CTACCTCCCC
    GCCACTGTGG AACCTGCCTC GCCCACCCCT GCCCATAGCC TGCCTCGTGC CAAGTCGGAG
    CTGACCCTAA CCCCAGACCC AGCCCCGCCC ATGGCCCACT CACCCCTGCA TCGTTCTGTG
    TCTGACCTGC CTCTTCCCCT GCCCCAGCCT GCCCTGGCCT CTCCTGGCTC GCCAGAACCC
    CTGCCCATCC TGTCCCTCAA CGGTGGGGGC CCAGAGCTGG CTGGGGACTG GGGTGGGGCT
    GGGGATGCTC CGCTGTCCCC GGACCCACTG CTGTCTTCAC CTCCTGGCTC TCCCAAGGCA
    GCTCTACACT CACCCGTCTC CGAAGGGGCC CCCGTAATTC CCCCATCCTC TGGGCTCCCA
    CTTCCCACCC CTGATGCCCG GGTAGGGGCC TCCACCTGCG GCCCGCCCGA CCACCTGCTG
    CCTCCGCTGG GCACCCCGCT GCCCCCAGAC TGGGTGACGC TGGAGGGGGA CTTTGTGCTC
    ATGTTGGCCA TCTCGCCCAG CCACCTAGGC GCTGACCTGG TGGCAGCTCC GCATGCGCGC
    TTCGACGACG GCCTGGTGCA CCTGTGCTGG GTGCGTAGCG GCATCTCGCG GGCTGCGCTG
    CTGCGCCTTT TCTTGGCCAT GGAGCGTGGT AGCCACTTCA GCCTGGGCTG TCCGCAGCTG
    GGCTACGCCG CGGCCCGTGC CTTCCGCCTA GAGCCGCTCA CACCACGCGG CGTGCTCACA
    GTGGACGGGG AGCAGGTGGA GTATGGGCCG CTACAGGCAC AGATGCACCC TGGCATCGGT
    ACACTGCTCA CTGGGCCTCC TGGCTGCCCG GGGCGGGAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16910
    Clone ID Related Accession (Same CDS sequence) NM_001204159.2 , XM_006723291.1 , NM_020126.5 , NM_020126.4 , NM_001204159.3 , XM_011527133.1
    Accession Version NM_001204159.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC022154.4, DB053266.1, AL136701.1 and AF245447.1. On May 31, 2019 this sequence version replaced NM_001204159.2. Transcript Variant: This variant (3) differs in the 5' UTR compared to variant 1. Variants 1 and 3 both encode the same isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL136701.1, SRR6380199.78259.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGATTTGAGC CAGCCCTGGG CCTCGACCTG
    TTGCTCAACT GCTCACTGTT GCTGTGCCGG GGTGGTGGCC ACCCACTGGA CCTGCTCTCC
    GTGACGCTGG CCTCGGGCTC CCGCTGTTTC TCCTTCCTGT CTGTGGCCTG GGGCTTCGTG
    TCAGATGTGG ATATCCAGAG CGAGCGCTTC AGGGCCTTGG GCAGTGCCCG CTTCACACTG
    GGCACGGTGC TGGGCCTCGC CACACTGCAC ACCTACCGCG GACGCCTCTC CTACCTCCCC
    GCCACTGTGG AACCTGCCTC GCCCACCCCT GCCCATAGCC TGCCTCGTGC CAAGTCGGAG
    CTGACCCTAA CCCCAGACCC AGCCCCGCCC ATGGCCCACT CACCCCTGCA TCGTTCTGTG
    TCTGACCTGC CTCTTCCCCT GCCCCAGCCT GCCCTGGCCT CTCCTGGCTC GCCAGAACCC
    CTGCCCATCC TGTCCCTCAA CGGTGGGGGC CCAGAGCTGG CTGGGGACTG GGGTGGGGCT
    GGGGATGCTC CGCTGTCCCC GGACCCACTG CTGTCTTCAC CTCCTGGCTC TCCCAAGGCA
    GCTCTACACT CACCCGTCTC CGAAGGGGCC CCCGTAATTC CCCCATCCTC TGGGCTCCCA
    CTTCCCACCC CTGATGCCCG GGTAGGGGCC TCCACCTGCG GCCCGCCCGA CCACCTGCTG
    CCTCCGCTGG GCACCCCGCT GCCCCCAGAC TGGGTGACGC TGGAGGGGGA CTTTGTGCTC
    ATGTTGGCCA TCTCGCCCAG CCACCTAGGC GCTGACCTGG TGGCAGCTCC GCATGCGCGC
    TTCGACGACG GCCTGGTGCA CCTGTGCTGG GTGCGTAGCG GCATCTCGCG GGCTGCGCTG
    CTGCGCCTTT TCTTGGCCAT GGAGCGTGGT AGCCACTTCA GCCTGGGCTG TCCGCAGCTG
    GGCTACGCCG CGGCCCGTGC CTTCCGCCTA GAGCCGCTCA CACCACGCGG CGTGCTCACA
    GTGGACGGGG AGCAGGTGGA GTATGGGCCG CTACAGGCAC AGATGCACCC TGGCATCGGT
    ACACTGCTCA CTGGGCCTCC TGGCTGCCCG GGGCGGGAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191088.1
    CDS298..2262
    Translation

    Target ORF information:

    RefSeq Version NM_001204159.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204159.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGATTTGAGC CAGCCCTGGG CCTCGACCTG
    TTGCTCAACT GCTCACTGTT GCTGTGCCGG GGTGGTGGCC ACCCACTGGA CCTGCTCTCC
    GTGACGCTGG CCTCGGGCTC CCGCTGTTTC TCCTTCCTGT CTGTGGCCTG GGGCTTCGTG
    TCAGATGTGG ATATCCAGAG CGAGCGCTTC AGGGCCTTGG GCAGTGCCCG CTTCACACTG
    GGCACGGTGC TGGGCCTCGC CACACTGCAC ACCTACCGCG GACGCCTCTC CTACCTCCCC
    GCCACTGTGG AACCTGCCTC GCCCACCCCT GCCCATAGCC TGCCTCGTGC CAAGTCGGAG
    CTGACCCTAA CCCCAGACCC AGCCCCGCCC ATGGCCCACT CACCCCTGCA TCGTTCTGTG
    TCTGACCTGC CTCTTCCCCT GCCCCAGCCT GCCCTGGCCT CTCCTGGCTC GCCAGAACCC
    CTGCCCATCC TGTCCCTCAA CGGTGGGGGC CCAGAGCTGG CTGGGGACTG GGGTGGGGCT
    GGGGATGCTC CGCTGTCCCC GGACCCACTG CTGTCTTCAC CTCCTGGCTC TCCCAAGGCA
    GCTCTACACT CACCCGTCTC CGAAGGGGCC CCCGTAATTC CCCCATCCTC TGGGCTCCCA
    CTTCCCACCC CTGATGCCCG GGTAGGGGCC TCCACCTGCG GCCCGCCCGA CCACCTGCTG
    CCTCCGCTGG GCACCCCGCT GCCCCCAGAC TGGGTGACGC TGGAGGGGGA CTTTGTGCTC
    ATGTTGGCCA TCTCGCCCAG CCACCTAGGC GCTGACCTGG TGGCAGCTCC GCATGCGCGC
    TTCGACGACG GCCTGGTGCA CCTGTGCTGG GTGCGTAGCG GCATCTCGCG GGCTGCGCTG
    CTGCGCCTTT TCTTGGCCAT GGAGCGTGGT AGCCACTTCA GCCTGGGCTG TCCGCAGCTG
    GGCTACGCCG CGGCCCGTGC CTTCCGCCTA GAGCCGCTCA CACCACGCGG CGTGCTCACA
    GTGGACGGGG AGCAGGTGGA GTATGGGCCG CTACAGGCAC AGATGCACCC TGGCATCGGT
    ACACTGCTCA CTGGGCCTCC TGGCTGCCCG GGGCGGGAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16897
    Clone ID Related Accession (Same CDS sequence) XM_006723292.1 , NM_001204160.3 , XM_011527134.1 , NM_001204160.2
    Accession Version XM_006723292.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCGC CCCCACCGCC ACTGGCTGCC AGCACCCCGC TCCTCCATGG CGAGTTTGGC 
    TCCTACCCAG CCCGAGGCCC ACGCTTTGCC CTCACCCTTA CATCGCAGGC CCTGCACATA
    CAGCGGCTGC GCCCCAAACC TGAAGCCAGG CCCCGGGGTG GCCTGGTCCC GTTGGCCGAG
    GTCTCAGGCT GCTGCACCCT GCGAAGCCGC AGCCCCTCAG ACTCAGCGGC CTACTTCTGC
    ATCTACACCT ACCCTCGGGG CCGGCGCGGG GCCCGGCGCA GAGCCACTCG CACCTTCCGG
    GCAGATGGGG CCGCCACCTA CGAAGAGAAC CGTGCCGAGG CCCAGCGCTG GGCCACTGCC
    CTCACCTGTC TGCTCCGAGG ACTGCCACTG CCCGGGGATG GGGAGATCAC CCCTGACCTG
    CTACCTCGGC CGCCCCGGTT GCTTCTATTG GTCAATCCCT TTGGGGGTCG GGGCCTGGCC
    TGGCAGTGGT GTAAGAACCA CGTGCTTCCC ATGATCTCTG AAGCTGGGCT GTCCTTCAAC
    CTCATCCAGA CAGAACGACA GAACCACGCC CGGGAGCTGG TCCAGGGGCT GAGCCTGAGT
    GAGTGGGATG GCATCGTCAC GGTCTCGGGA GACGGGCTGC TCCATGAGGT GCTGAACGGG
    CTCCTAGATC GCCCTGACTG GGAGGAAGCT GTGAAGATGC CTGTGGGCAT CCTCCCCTGC
    GGCTCGGGCA ACGCGCTGGC CGGAGCAGTG AACCAGCACG GGGGATTTGA GCCAGCCCTG
    GGCCTCGACC TGTTGCTCAA CTGCTCACTG TTGCTGTGCC GGGGTGGTGG CCACCCACTG
    GACCTGCTCT CCGTGACGCT GGCCTCGGGC TCCCGCTGTT TCTCCTTCCT GTCTGTGGCC
    TGGGGCTTCG TGTCAGATGT GGATATCCAG AGCGAGCGCT TCAGGGCCTT GGGCAGTGCC
    CGCTTCACAC TGGGCACGGT GCTGGGCCTC GCCACACTGC ACACCTACCG CGGACGCCTC
    TCCTACCTCC CCGCCACTGT GGAACCTGCC TCGCCCACCC CTGCCCATAG CCTGCCTCGT
    GCCAAGTCGG AGCTGACCCT AACCCCAGAC CCAGCCCCGC CCATGGCCCA CTCACCCCTG
    CATCGTTCTG TGTCTGACCT GCCTCTTCCC CTGCCCCAGC CTGCCCTGGC CTCTCCTGGC
    TCGCCAGAAC CCCTGCCCAT CCTGTCCCTC AACGGTGGGG GCCCAGAGCT GGCTGGGGAC
    TGGGGTGGGG CTGGGGATGC TCCGCTGTCC CCGGACCCAC TGCTGTCTTC ACCTCCTGGC
    TCTCCCAAGG CAGCTCTACA CTCACCCGTC TCCGAAGGGG CCCCCGTAAT TCCCCCATCC
    TCTGGGCTCC CACTTCCCAC CCCTGATGCC CGGGTAGGGG CCTCCACCTG CGGCCCGCCC
    GACCACCTGC TGCCTCCGCT GGGCACCCCG CTGCCCCCAG ACTGGGTGAC GCTGGAGGGG
    GACTTTGTGC TCATGTTGGC CATCTCGCCC AGCCACCTAG GCGCTGACCT GGTGGCAGCT
    CCGCATGCGC GCTTCGACGA CGGCCTGGTG CACCTGTGCT GGGTGCGTAG CGGCATCTCG
    CGGGCTGCGC TGCTGCGCCT TTTCTTGGCC ATGGAGCGTG GTAGCCACTT CAGCCTGGGC
    TGTCCGCAGC TGGGCTACGC CGCGGCCCGT GCCTTCCGCC TAGAGCCGCT CACACCACGC
    GGCGTGCTCA CAGTGGACGG GGAGCAGGTG GAGTATGGGC CGCTACAGGC ACAGATGCAC
    CCTGGCATCG GTACACTGCT CACTGGGCCT CCTGGCTGCC CGGGGCGGGA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006723355.1
    CDS165..2021
    Translation

    Target ORF information:

    RefSeq Version XM_006723292.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006723292.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCGC CCCCACCGCC ACTGGCTGCC AGCACCCCGC TCCTCCATGG CGAGTTTGGC 
    TCCTACCCAG CCCGAGGCCC ACGCTTTGCC CTCACCCTTA CATCGCAGGC CCTGCACATA
    CAGCGGCTGC GCCCCAAACC TGAAGCCAGG CCCCGGGGTG GCCTGGTCCC GTTGGCCGAG
    GTCTCAGGCT GCTGCACCCT GCGAAGCCGC AGCCCCTCAG ACTCAGCGGC CTACTTCTGC
    ATCTACACCT ACCCTCGGGG CCGGCGCGGG GCCCGGCGCA GAGCCACTCG CACCTTCCGG
    GCAGATGGGG CCGCCACCTA CGAAGAGAAC CGTGCCGAGG CCCAGCGCTG GGCCACTGCC
    CTCACCTGTC TGCTCCGAGG ACTGCCACTG CCCGGGGATG GGGAGATCAC CCCTGACCTG
    CTACCTCGGC CGCCCCGGTT GCTTCTATTG GTCAATCCCT TTGGGGGTCG GGGCCTGGCC
    TGGCAGTGGT GTAAGAACCA CGTGCTTCCC ATGATCTCTG AAGCTGGGCT GTCCTTCAAC
    CTCATCCAGA CAGAACGACA GAACCACGCC CGGGAGCTGG TCCAGGGGCT GAGCCTGAGT
    GAGTGGGATG GCATCGTCAC GGTCTCGGGA GACGGGCTGC TCCATGAGGT GCTGAACGGG
    CTCCTAGATC GCCCTGACTG GGAGGAAGCT GTGAAGATGC CTGTGGGCAT CCTCCCCTGC
    GGCTCGGGCA ACGCGCTGGC CGGAGCAGTG AACCAGCACG GGGGATTTGA GCCAGCCCTG
    GGCCTCGACC TGTTGCTCAA CTGCTCACTG TTGCTGTGCC GGGGTGGTGG CCACCCACTG
    GACCTGCTCT CCGTGACGCT GGCCTCGGGC TCCCGCTGTT TCTCCTTCCT GTCTGTGGCC
    TGGGGCTTCG TGTCAGATGT GGATATCCAG AGCGAGCGCT TCAGGGCCTT GGGCAGTGCC
    CGCTTCACAC TGGGCACGGT GCTGGGCCTC GCCACACTGC ACACCTACCG CGGACGCCTC
    TCCTACCTCC CCGCCACTGT GGAACCTGCC TCGCCCACCC CTGCCCATAG CCTGCCTCGT
    GCCAAGTCGG AGCTGACCCT AACCCCAGAC CCAGCCCCGC CCATGGCCCA CTCACCCCTG
    CATCGTTCTG TGTCTGACCT GCCTCTTCCC CTGCCCCAGC CTGCCCTGGC CTCTCCTGGC
    TCGCCAGAAC CCCTGCCCAT CCTGTCCCTC AACGGTGGGG GCCCAGAGCT GGCTGGGGAC
    TGGGGTGGGG CTGGGGATGC TCCGCTGTCC CCGGACCCAC TGCTGTCTTC ACCTCCTGGC
    TCTCCCAAGG CAGCTCTACA CTCACCCGTC TCCGAAGGGG CCCCCGTAAT TCCCCCATCC
    TCTGGGCTCC CACTTCCCAC CCCTGATGCC CGGGTAGGGG CCTCCACCTG CGGCCCGCCC
    GACCACCTGC TGCCTCCGCT GGGCACCCCG CTGCCCCCAG ACTGGGTGAC GCTGGAGGGG
    GACTTTGTGC TCATGTTGGC CATCTCGCCC AGCCACCTAG GCGCTGACCT GGTGGCAGCT
    CCGCATGCGC GCTTCGACGA CGGCCTGGTG CACCTGTGCT GGGTGCGTAG CGGCATCTCG
    CGGGCTGCGC TGCTGCGCCT TTTCTTGGCC ATGGAGCGTG GTAGCCACTT CAGCCTGGGC
    TGTCCGCAGC TGGGCTACGC CGCGGCCCGT GCCTTCCGCC TAGAGCCGCT CACACCACGC
    GGCGTGCTCA CAGTGGACGG GGAGCAGGTG GAGTATGGGC CGCTACAGGC ACAGATGCAC
    CCTGGCATCG GTACACTGCT CACTGGGCCT CCTGGCTGCC CGGGGCGGGA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16897
    Clone ID Related Accession (Same CDS sequence) XM_006723292.1 , NM_001204160.3 , XM_011527134.1 , NM_001204160.2
    Accession Version XM_011527134.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1857bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCGC CCCCACCGCC ACTGGCTGCC AGCACCCCGC TCCTCCATGG CGAGTTTGGC 
    TCCTACCCAG CCCGAGGCCC ACGCTTTGCC CTCACCCTTA CATCGCAGGC CCTGCACATA
    CAGCGGCTGC GCCCCAAACC TGAAGCCAGG CCCCGGGGTG GCCTGGTCCC GTTGGCCGAG
    GTCTCAGGCT GCTGCACCCT GCGAAGCCGC AGCCCCTCAG ACTCAGCGGC CTACTTCTGC
    ATCTACACCT ACCCTCGGGG CCGGCGCGGG GCCCGGCGCA GAGCCACTCG CACCTTCCGG
    GCAGATGGGG CCGCCACCTA CGAAGAGAAC CGTGCCGAGG CCCAGCGCTG GGCCACTGCC
    CTCACCTGTC TGCTCCGAGG ACTGCCACTG CCCGGGGATG GGGAGATCAC CCCTGACCTG
    CTACCTCGGC CGCCCCGGTT GCTTCTATTG GTCAATCCCT TTGGGGGTCG GGGCCTGGCC
    TGGCAGTGGT GTAAGAACCA CGTGCTTCCC ATGATCTCTG AAGCTGGGCT GTCCTTCAAC
    CTCATCCAGA CAGAACGACA GAACCACGCC CGGGAGCTGG TCCAGGGGCT GAGCCTGAGT
    GAGTGGGATG GCATCGTCAC GGTCTCGGGA GACGGGCTGC TCCATGAGGT GCTGAACGGG
    CTCCTAGATC GCCCTGACTG GGAGGAAGCT GTGAAGATGC CTGTGGGCAT CCTCCCCTGC
    GGCTCGGGCA ACGCGCTGGC CGGAGCAGTG AACCAGCACG GGGGATTTGA GCCAGCCCTG
    GGCCTCGACC TGTTGCTCAA CTGCTCACTG TTGCTGTGCC GGGGTGGTGG CCACCCACTG
    GACCTGCTCT CCGTGACGCT GGCCTCGGGC TCCCGCTGTT TCTCCTTCCT GTCTGTGGCC
    TGGGGCTTCG TGTCAGATGT GGATATCCAG AGCGAGCGCT TCAGGGCCTT GGGCAGTGCC
    CGCTTCACAC TGGGCACGGT GCTGGGCCTC GCCACACTGC ACACCTACCG CGGACGCCTC
    TCCTACCTCC CCGCCACTGT GGAACCTGCC TCGCCCACCC CTGCCCATAG CCTGCCTCGT
    GCCAAGTCGG AGCTGACCCT AACCCCAGAC CCAGCCCCGC CCATGGCCCA CTCACCCCTG
    CATCGTTCTG TGTCTGACCT GCCTCTTCCC CTGCCCCAGC CTGCCCTGGC CTCTCCTGGC
    TCGCCAGAAC CCCTGCCCAT CCTGTCCCTC AACGGTGGGG GCCCAGAGCT GGCTGGGGAC
    TGGGGTGGGG CTGGGGATGC TCCGCTGTCC CCGGACCCAC TGCTGTCTTC ACCTCCTGGC
    TCTCCCAAGG CAGCTCTACA CTCACCCGTC TCCGAAGGGG CCCCCGTAAT TCCCCCATCC
    TCTGGGCTCC CACTTCCCAC CCCTGATGCC CGGGTAGGGG CCTCCACCTG CGGCCCGCCC
    GACCACCTGC TGCCTCCGCT GGGCACCCCG CTGCCCCCAG ACTGGGTGAC GCTGGAGGGG
    GACTTTGTGC TCATGTTGGC CATCTCGCCC AGCCACCTAG GCGCTGACCT GGTGGCAGCT
    CCGCATGCGC GCTTCGACGA CGGCCTGGTG CACCTGTGCT GGGTGCGTAG CGGCATCTCG
    CGGGCTGCGC TGCTGCGCCT TTTCTTGGCC ATGGAGCGTG GTAGCCACTT CAGCCTGGGC
    TGTCCGCAGC TGGGCTACGC CGCGGCCCGT GCCTTCCGCC TAGAGCCGCT CACACCACGC
    GGCGTGCTCA CAGTGGACGG GGAGCAGGTG GAGTATGGGC CGCTACAGGC ACAGATGCAC
    CCTGGCATCG GTACACTGCT CACTGGGCCT CCTGGCTGCC CGGGGCGGGA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011525436.1
    CDS167..2023
    Translation

    Target ORF information:

    RefSeq Version XM_011527134.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011527134.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    ATGGCCCCGC CCCCACCGCC ACTGGCTGCC AGCACCCCGC TCCTCCATGG CGAGTTTGGC 
    TCCTACCCAG CCCGAGGCCC ACGCTTTGCC CTCACCCTTA CATCGCAGGC CCTGCACATA
    CAGCGGCTGC GCCCCAAACC TGAAGCCAGG CCCCGGGGTG GCCTGGTCCC GTTGGCCGAG
    GTCTCAGGCT GCTGCACCCT GCGAAGCCGC AGCCCCTCAG ACTCAGCGGC CTACTTCTGC
    ATCTACACCT ACCCTCGGGG CCGGCGCGGG GCCCGGCGCA GAGCCACTCG CACCTTCCGG
    GCAGATGGGG CCGCCACCTA CGAAGAGAAC CGTGCCGAGG CCCAGCGCTG GGCCACTGCC
    CTCACCTGTC TGCTCCGAGG ACTGCCACTG CCCGGGGATG GGGAGATCAC CCCTGACCTG
    CTACCTCGGC CGCCCCGGTT GCTTCTATTG GTCAATCCCT TTGGGGGTCG GGGCCTGGCC
    TGGCAGTGGT GTAAGAACCA CGTGCTTCCC ATGATCTCTG AAGCTGGGCT GTCCTTCAAC
    CTCATCCAGA CAGAACGACA GAACCACGCC CGGGAGCTGG TCCAGGGGCT GAGCCTGAGT
    GAGTGGGATG GCATCGTCAC GGTCTCGGGA GACGGGCTGC TCCATGAGGT GCTGAACGGG
    CTCCTAGATC GCCCTGACTG GGAGGAAGCT GTGAAGATGC CTGTGGGCAT CCTCCCCTGC
    GGCTCGGGCA ACGCGCTGGC CGGAGCAGTG AACCAGCACG GGGGATTTGA GCCAGCCCTG
    GGCCTCGACC TGTTGCTCAA CTGCTCACTG TTGCTGTGCC GGGGTGGTGG CCACCCACTG
    GACCTGCTCT CCGTGACGCT GGCCTCGGGC TCCCGCTGTT TCTCCTTCCT GTCTGTGGCC
    TGGGGCTTCG TGTCAGATGT GGATATCCAG AGCGAGCGCT TCAGGGCCTT GGGCAGTGCC
    CGCTTCACAC TGGGCACGGT GCTGGGCCTC GCCACACTGC ACACCTACCG CGGACGCCTC
    TCCTACCTCC CCGCCACTGT GGAACCTGCC TCGCCCACCC CTGCCCATAG CCTGCCTCGT
    GCCAAGTCGG AGCTGACCCT AACCCCAGAC CCAGCCCCGC CCATGGCCCA CTCACCCCTG
    CATCGTTCTG TGTCTGACCT GCCTCTTCCC CTGCCCCAGC CTGCCCTGGC CTCTCCTGGC
    TCGCCAGAAC CCCTGCCCAT CCTGTCCCTC AACGGTGGGG GCCCAGAGCT GGCTGGGGAC
    TGGGGTGGGG CTGGGGATGC TCCGCTGTCC CCGGACCCAC TGCTGTCTTC ACCTCCTGGC
    TCTCCCAAGG CAGCTCTACA CTCACCCGTC TCCGAAGGGG CCCCCGTAAT TCCCCCATCC
    TCTGGGCTCC CACTTCCCAC CCCTGATGCC CGGGTAGGGG CCTCCACCTG CGGCCCGCCC
    GACCACCTGC TGCCTCCGCT GGGCACCCCG CTGCCCCCAG ACTGGGTGAC GCTGGAGGGG
    GACTTTGTGC TCATGTTGGC CATCTCGCCC AGCCACCTAG GCGCTGACCT GGTGGCAGCT
    CCGCATGCGC GCTTCGACGA CGGCCTGGTG CACCTGTGCT GGGTGCGTAG CGGCATCTCG
    CGGGCTGCGC TGCTGCGCCT TTTCTTGGCC ATGGAGCGTG GTAGCCACTT CAGCCTGGGC
    TGTCCGCAGC TGGGCTACGC CGCGGCCCGT GCCTTCCGCC TAGAGCCGCT CACACCACGC
    GGCGTGCTCA CAGTGGACGG GGAGCAGGTG GAGTATGGGC CGCTACAGGC ACAGATGCAC
    CCTGGCATCG GTACACTGCT CACTGGGCCT CCTGGCTGCC CGGGGCGGGA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16910
    Clone ID Related Accession (Same CDS sequence) NM_001204159.2 , XM_006723291.1 , NM_020126.5 , NM_020126.4 , NM_001204159.3 , XM_011527133.1
    Accession Version XM_006723291.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-03-11
    Organism Homo sapiens(Human)
    Product sphingosine kinase 2 isoform X1
    Comment

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGATTTGAGC CAGCCCTGGG CCTCGACCTG
    TTGCTCAACT GCTCACTGTT GCTGTGCCGG GGTGGTGGCC ACCCACTGGA CCTGCTCTCC
    GTGACGCTGG CCTCGGGCTC CCGCTGTTTC TCCTTCCTGT CTGTGGCCTG GGGCTTCGTG
    TCAGATGTGG ATATCCAGAG CGAGCGCTTC AGGGCCTTGG GCAGTGCCCG CTTCACACTG
    GGCACGGTGC TGGGCCTCGC CACACTGCAC ACCTACCGCG GACGCCTCTC CTACCTCCCC
    GCCACTGTGG AACCTGCCTC GCCCACCCCT GCCCATAGCC TGCCTCGTGC CAAGTCGGAG
    CTGACCCTAA CCCCAGACCC AGCCCCGCCC ATGGCCCACT CACCCCTGCA TCGTTCTGTG
    TCTGACCTGC CTCTTCCCCT GCCCCAGCCT GCCCTGGCCT CTCCTGGCTC GCCAGAACCC
    CTGCCCATCC TGTCCCTCAA CGGTGGGGGC CCAGAGCTGG CTGGGGACTG GGGTGGGGCT
    GGGGATGCTC CGCTGTCCCC GGACCCACTG CTGTCTTCAC CTCCTGGCTC TCCCAAGGCA
    GCTCTACACT CACCCGTCTC CGAAGGGGCC CCCGTAATTC CCCCATCCTC TGGGCTCCCA
    CTTCCCACCC CTGATGCCCG GGTAGGGGCC TCCACCTGCG GCCCGCCCGA CCACCTGCTG
    CCTCCGCTGG GCACCCCGCT GCCCCCAGAC TGGGTGACGC TGGAGGGGGA CTTTGTGCTC
    ATGTTGGCCA TCTCGCCCAG CCACCTAGGC GCTGACCTGG TGGCAGCTCC GCATGCGCGC
    TTCGACGACG GCCTGGTGCA CCTGTGCTGG GTGCGTAGCG GCATCTCGCG GGCTGCGCTG
    CTGCGCCTTT TCTTGGCCAT GGAGCGTGGT AGCCACTTCA GCCTGGGCTG TCCGCAGCTG
    GGCTACGCCG CGGCCCGTGC CTTCCGCCTA GAGCCGCTCA CACCACGCGG CGTGCTCACA
    GTGGACGGGG AGCAGGTGGA GTATGGGCCG CTACAGGCAC AGATGCACCC TGGCATCGGT
    ACACTGCTCA CTGGGCCTCC TGGCTGCCCG GGGCGGGAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_006723354.1
    Translation

    Target ORF information:

    RefSeq Version XM_006723291.1
    Organism Homo sapiens(Human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_006723291.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGATTTGAGC CAGCCCTGGG CCTCGACCTG
    TTGCTCAACT GCTCACTGTT GCTGTGCCGG GGTGGTGGCC ACCCACTGGA CCTGCTCTCC
    GTGACGCTGG CCTCGGGCTC CCGCTGTTTC TCCTTCCTGT CTGTGGCCTG GGGCTTCGTG
    TCAGATGTGG ATATCCAGAG CGAGCGCTTC AGGGCCTTGG GCAGTGCCCG CTTCACACTG
    GGCACGGTGC TGGGCCTCGC CACACTGCAC ACCTACCGCG GACGCCTCTC CTACCTCCCC
    GCCACTGTGG AACCTGCCTC GCCCACCCCT GCCCATAGCC TGCCTCGTGC CAAGTCGGAG
    CTGACCCTAA CCCCAGACCC AGCCCCGCCC ATGGCCCACT CACCCCTGCA TCGTTCTGTG
    TCTGACCTGC CTCTTCCCCT GCCCCAGCCT GCCCTGGCCT CTCCTGGCTC GCCAGAACCC
    CTGCCCATCC TGTCCCTCAA CGGTGGGGGC CCAGAGCTGG CTGGGGACTG GGGTGGGGCT
    GGGGATGCTC CGCTGTCCCC GGACCCACTG CTGTCTTCAC CTCCTGGCTC TCCCAAGGCA
    GCTCTACACT CACCCGTCTC CGAAGGGGCC CCCGTAATTC CCCCATCCTC TGGGCTCCCA
    CTTCCCACCC CTGATGCCCG GGTAGGGGCC TCCACCTGCG GCCCGCCCGA CCACCTGCTG
    CCTCCGCTGG GCACCCCGCT GCCCCCAGAC TGGGTGACGC TGGAGGGGGA CTTTGTGCTC
    ATGTTGGCCA TCTCGCCCAG CCACCTAGGC GCTGACCTGG TGGCAGCTCC GCATGCGCGC
    TTCGACGACG GCCTGGTGCA CCTGTGCTGG GTGCGTAGCG GCATCTCGCG GGCTGCGCTG
    CTGCGCCTTT TCTTGGCCAT GGAGCGTGGT AGCCACTTCA GCCTGGGCTG TCCGCAGCTG
    GGCTACGCCG CGGCCCGTGC CTTCCGCCTA GAGCCGCTCA CACCACGCGG CGTGCTCACA
    GTGGACGGGG AGCAGGTGGA GTATGGGCCG CTACAGGCAC AGATGCACCC TGGCATCGGT
    ACACTGCTCA CTGGGCCTCC TGGCTGCCCG GGGCGGGAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16910
    Clone ID Related Accession (Same CDS sequence) NM_001204159.2 , XM_006723291.1 , NM_020126.5 , NM_020126.4 , NM_001204159.3 , XM_011527133.1
    Accession Version XM_011527133.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1965bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGATTTGAGC CAGCCCTGGG CCTCGACCTG
    TTGCTCAACT GCTCACTGTT GCTGTGCCGG GGTGGTGGCC ACCCACTGGA CCTGCTCTCC
    GTGACGCTGG CCTCGGGCTC CCGCTGTTTC TCCTTCCTGT CTGTGGCCTG GGGCTTCGTG
    TCAGATGTGG ATATCCAGAG CGAGCGCTTC AGGGCCTTGG GCAGTGCCCG CTTCACACTG
    GGCACGGTGC TGGGCCTCGC CACACTGCAC ACCTACCGCG GACGCCTCTC CTACCTCCCC
    GCCACTGTGG AACCTGCCTC GCCCACCCCT GCCCATAGCC TGCCTCGTGC CAAGTCGGAG
    CTGACCCTAA CCCCAGACCC AGCCCCGCCC ATGGCCCACT CACCCCTGCA TCGTTCTGTG
    TCTGACCTGC CTCTTCCCCT GCCCCAGCCT GCCCTGGCCT CTCCTGGCTC GCCAGAACCC
    CTGCCCATCC TGTCCCTCAA CGGTGGGGGC CCAGAGCTGG CTGGGGACTG GGGTGGGGCT
    GGGGATGCTC CGCTGTCCCC GGACCCACTG CTGTCTTCAC CTCCTGGCTC TCCCAAGGCA
    GCTCTACACT CACCCGTCTC CGAAGGGGCC CCCGTAATTC CCCCATCCTC TGGGCTCCCA
    CTTCCCACCC CTGATGCCCG GGTAGGGGCC TCCACCTGCG GCCCGCCCGA CCACCTGCTG
    CCTCCGCTGG GCACCCCGCT GCCCCCAGAC TGGGTGACGC TGGAGGGGGA CTTTGTGCTC
    ATGTTGGCCA TCTCGCCCAG CCACCTAGGC GCTGACCTGG TGGCAGCTCC GCATGCGCGC
    TTCGACGACG GCCTGGTGCA CCTGTGCTGG GTGCGTAGCG GCATCTCGCG GGCTGCGCTG
    CTGCGCCTTT TCTTGGCCAT GGAGCGTGGT AGCCACTTCA GCCTGGGCTG TCCGCAGCTG
    GGCTACGCCG CGGCCCGTGC CTTCCGCCTA GAGCCGCTCA CACCACGCGG CGTGCTCACA
    GTGGACGGGG AGCAGGTGGA GTATGGGCCG CTACAGGCAC AGATGCACCC TGGCATCGGT
    ACACTGCTCA CTGGGCCTCC TGGCTGCCCG GGGCGGGAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011525435.1
    CDS202..2166
    Translation

    Target ORF information:

    RefSeq Version XM_011527133.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011527133.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGATTTGAGC CAGCCCTGGG CCTCGACCTG
    TTGCTCAACT GCTCACTGTT GCTGTGCCGG GGTGGTGGCC ACCCACTGGA CCTGCTCTCC
    GTGACGCTGG CCTCGGGCTC CCGCTGTTTC TCCTTCCTGT CTGTGGCCTG GGGCTTCGTG
    TCAGATGTGG ATATCCAGAG CGAGCGCTTC AGGGCCTTGG GCAGTGCCCG CTTCACACTG
    GGCACGGTGC TGGGCCTCGC CACACTGCAC ACCTACCGCG GACGCCTCTC CTACCTCCCC
    GCCACTGTGG AACCTGCCTC GCCCACCCCT GCCCATAGCC TGCCTCGTGC CAAGTCGGAG
    CTGACCCTAA CCCCAGACCC AGCCCCGCCC ATGGCCCACT CACCCCTGCA TCGTTCTGTG
    TCTGACCTGC CTCTTCCCCT GCCCCAGCCT GCCCTGGCCT CTCCTGGCTC GCCAGAACCC
    CTGCCCATCC TGTCCCTCAA CGGTGGGGGC CCAGAGCTGG CTGGGGACTG GGGTGGGGCT
    GGGGATGCTC CGCTGTCCCC GGACCCACTG CTGTCTTCAC CTCCTGGCTC TCCCAAGGCA
    GCTCTACACT CACCCGTCTC CGAAGGGGCC CCCGTAATTC CCCCATCCTC TGGGCTCCCA
    CTTCCCACCC CTGATGCCCG GGTAGGGGCC TCCACCTGCG GCCCGCCCGA CCACCTGCTG
    CCTCCGCTGG GCACCCCGCT GCCCCCAGAC TGGGTGACGC TGGAGGGGGA CTTTGTGCTC
    ATGTTGGCCA TCTCGCCCAG CCACCTAGGC GCTGACCTGG TGGCAGCTCC GCATGCGCGC
    TTCGACGACG GCCTGGTGCA CCTGTGCTGG GTGCGTAGCG GCATCTCGCG GGCTGCGCTG
    CTGCGCCTTT TCTTGGCCAT GGAGCGTGGT AGCCACTTCA GCCTGGGCTG TCCGCAGCTG
    GGCTACGCCG CGGCCCGTGC CTTCCGCCTA GAGCCGCTCA CACCACGCGG CGTGCTCACA
    GTGGACGGGG AGCAGGTGGA GTATGGGCCG CTACAGGCAC AGATGCACCC TGGCATCGGT
    ACACTGCTCA CTGGGCCTCC TGGCTGCCCG GGGCGGGAGC CCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16837
    Clone ID Related Accession (Same CDS sequence) XM_017027009.1 , NM_001243876.1
    Accession Version NM_001243876.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-24
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform d
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI224962.1, DA926908.1, AK122735.1 and DB367710.1. Transcript Variant: This variant (5) lacks an exon in the coding region and initiates translation at a downstream, in-frame start codon, compared to variant 1. The encoded isoform (d) has a shorter N-terminus, compared to isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2142853 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATCTCTG AAGCTGGGCT GTCCTTCAAC CTCATCCAGA CAGAACGACA GAACCACGCC 
    CGGGAGCTGG TCCAGGGGCT GAGCCTGAGT GAGTGGGATG GCATCGTCAC GGTCTCGGGA
    GACGGGCTGC TCCATGAGGT GCTGAACGGG CTCCTAGATC GCCCTGACTG GGAGGAAGCT
    GTGAAGATGC CTGTGGGCAT CCTCCCCTGC GGCTCGGGCA ACGCGCTGGC CGGAGCAGTG
    AACCAGCACG GGGGATTTGA GCCAGCCCTG GGCCTCGACC TGTTGCTCAA CTGCTCACTG
    TTGCTGTGCC GGGGTGGTGG CCACCCACTG GACCTGCTCT CCGTGACGCT GGCCTCGGGC
    TCCCGCTGTT TCTCCTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGATATCCAG
    AGCGAGCGCT TCAGGGCCTT GGGCAGTGCC CGCTTCACAC TGGGCACGGT GCTGGGCCTC
    GCCACACTGC ACACCTACCG CGGACGCCTC TCCTACCTCC CCGCCACTGT GGAACCTGCC
    TCGCCCACCC CTGCCCATAG CCTGCCTCGT GCCAAGTCGG AGCTGACCCT AACCCCAGAC
    CCAGCCCCGC CCATGGCCCA CTCACCCCTG CATCGTTCTG TGTCTGACCT GCCTCTTCCC
    CTGCCCCAGC CTGCCCTGGC CTCTCCTGGC TCGCCAGAAC CCCTGCCCAT CCTGTCCCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC TCCGCTGTCC
    CCGGACCCAC TGCTGTCTTC ACCTCCTGGC TCTCCCAAGG CAGCTCTACA CTCACCCGTC
    TCCGAAGGGG CCCCCGTAAT TCCCCCATCC TCTGGGCTCC CACTTCCCAC CCCTGATGCC
    CGGGTAGGGG CCTCCACCTG CGGCCCGCCC GACCACCTGC TGCCTCCGCT GGGCACCCCG
    CTGCCCCCAG ACTGGGTGAC GCTGGAGGGG GACTTTGTGC TCATGTTGGC CATCTCGCCC
    AGCCACCTAG GCGCTGACCT GGTGGCAGCT CCGCATGCGC GCTTCGACGA CGGCCTGGTG
    CACCTGTGCT GGGTGCGTAG CGGCATCTCG CGGGCTGCGC TGCTGCGCCT TTTCTTGGCC
    ATGGAGCGTG GTAGCCACTT CAGCCTGGGC TGTCCGCAGC TGGGCTACGC CGCGGCCCGT
    GCCTTCCGCC TAGAGCCGCT CACACCACGC GGCGTGCTCA CAGTGGACGG GGAGCAGGTG
    GAGTATGGGC CGCTACAGGC ACAGATGCAC CCTGGCATCG GTACACTGCT CACTGGGCCT
    CCTGGCTGCC CGGGGCGGGA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001230805.1
    CDS513..1859
    Translation

    Target ORF information:

    RefSeq Version NM_001243876.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001243876.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATCTCTG AAGCTGGGCT GTCCTTCAAC CTCATCCAGA CAGAACGACA GAACCACGCC 
    CGGGAGCTGG TCCAGGGGCT GAGCCTGAGT GAGTGGGATG GCATCGTCAC GGTCTCGGGA
    GACGGGCTGC TCCATGAGGT GCTGAACGGG CTCCTAGATC GCCCTGACTG GGAGGAAGCT
    GTGAAGATGC CTGTGGGCAT CCTCCCCTGC GGCTCGGGCA ACGCGCTGGC CGGAGCAGTG
    AACCAGCACG GGGGATTTGA GCCAGCCCTG GGCCTCGACC TGTTGCTCAA CTGCTCACTG
    TTGCTGTGCC GGGGTGGTGG CCACCCACTG GACCTGCTCT CCGTGACGCT GGCCTCGGGC
    TCCCGCTGTT TCTCCTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGATATCCAG
    AGCGAGCGCT TCAGGGCCTT GGGCAGTGCC CGCTTCACAC TGGGCACGGT GCTGGGCCTC
    GCCACACTGC ACACCTACCG CGGACGCCTC TCCTACCTCC CCGCCACTGT GGAACCTGCC
    TCGCCCACCC CTGCCCATAG CCTGCCTCGT GCCAAGTCGG AGCTGACCCT AACCCCAGAC
    CCAGCCCCGC CCATGGCCCA CTCACCCCTG CATCGTTCTG TGTCTGACCT GCCTCTTCCC
    CTGCCCCAGC CTGCCCTGGC CTCTCCTGGC TCGCCAGAAC CCCTGCCCAT CCTGTCCCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC TCCGCTGTCC
    CCGGACCCAC TGCTGTCTTC ACCTCCTGGC TCTCCCAAGG CAGCTCTACA CTCACCCGTC
    TCCGAAGGGG CCCCCGTAAT TCCCCCATCC TCTGGGCTCC CACTTCCCAC CCCTGATGCC
    CGGGTAGGGG CCTCCACCTG CGGCCCGCCC GACCACCTGC TGCCTCCGCT GGGCACCCCG
    CTGCCCCCAG ACTGGGTGAC GCTGGAGGGG GACTTTGTGC TCATGTTGGC CATCTCGCCC
    AGCCACCTAG GCGCTGACCT GGTGGCAGCT CCGCATGCGC GCTTCGACGA CGGCCTGGTG
    CACCTGTGCT GGGTGCGTAG CGGCATCTCG CGGGCTGCGC TGCTGCGCCT TTTCTTGGCC
    ATGGAGCGTG GTAGCCACTT CAGCCTGGGC TGTCCGCAGC TGGGCTACGC CGCGGCCCGT
    GCCTTCCGCC TAGAGCCGCT CACACCACGC GGCGTGCTCA CAGTGGACGG GGAGCAGGTG
    GAGTATGGGC CGCTACAGGC ACAGATGCAC CCTGGCATCG GTACACTGCT CACTGGGCCT
    CCTGGCTGCC CGGGGCGGGA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16837
    Clone ID Related Accession (Same CDS sequence) XM_017027009.1 , NM_001243876.1
    Accession Version XM_017027009.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1347bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATCTCTG AAGCTGGGCT GTCCTTCAAC CTCATCCAGA CAGAACGACA GAACCACGCC 
    CGGGAGCTGG TCCAGGGGCT GAGCCTGAGT GAGTGGGATG GCATCGTCAC GGTCTCGGGA
    GACGGGCTGC TCCATGAGGT GCTGAACGGG CTCCTAGATC GCCCTGACTG GGAGGAAGCT
    GTGAAGATGC CTGTGGGCAT CCTCCCCTGC GGCTCGGGCA ACGCGCTGGC CGGAGCAGTG
    AACCAGCACG GGGGATTTGA GCCAGCCCTG GGCCTCGACC TGTTGCTCAA CTGCTCACTG
    TTGCTGTGCC GGGGTGGTGG CCACCCACTG GACCTGCTCT CCGTGACGCT GGCCTCGGGC
    TCCCGCTGTT TCTCCTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGATATCCAG
    AGCGAGCGCT TCAGGGCCTT GGGCAGTGCC CGCTTCACAC TGGGCACGGT GCTGGGCCTC
    GCCACACTGC ACACCTACCG CGGACGCCTC TCCTACCTCC CCGCCACTGT GGAACCTGCC
    TCGCCCACCC CTGCCCATAG CCTGCCTCGT GCCAAGTCGG AGCTGACCCT AACCCCAGAC
    CCAGCCCCGC CCATGGCCCA CTCACCCCTG CATCGTTCTG TGTCTGACCT GCCTCTTCCC
    CTGCCCCAGC CTGCCCTGGC CTCTCCTGGC TCGCCAGAAC CCCTGCCCAT CCTGTCCCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC TCCGCTGTCC
    CCGGACCCAC TGCTGTCTTC ACCTCCTGGC TCTCCCAAGG CAGCTCTACA CTCACCCGTC
    TCCGAAGGGG CCCCCGTAAT TCCCCCATCC TCTGGGCTCC CACTTCCCAC CCCTGATGCC
    CGGGTAGGGG CCTCCACCTG CGGCCCGCCC GACCACCTGC TGCCTCCGCT GGGCACCCCG
    CTGCCCCCAG ACTGGGTGAC GCTGGAGGGG GACTTTGTGC TCATGTTGGC CATCTCGCCC
    AGCCACCTAG GCGCTGACCT GGTGGCAGCT CCGCATGCGC GCTTCGACGA CGGCCTGGTG
    CACCTGTGCT GGGTGCGTAG CGGCATCTCG CGGGCTGCGC TGCTGCGCCT TTTCTTGGCC
    ATGGAGCGTG GTAGCCACTT CAGCCTGGGC TGTCCGCAGC TGGGCTACGC CGCGGCCCGT
    GCCTTCCGCC TAGAGCCGCT CACACCACGC GGCGTGCTCA CAGTGGACGG GGAGCAGGTG
    GAGTATGGGC CGCTACAGGC ACAGATGCAC CCTGGCATCG GTACACTGCT CACTGGGCCT
    CCTGGCTGCC CGGGGCGGGA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016882498.1
    CDS396..1742
    Translation

    Target ORF information:

    RefSeq Version XM_017027009.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027009.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    ATGATCTCTG AAGCTGGGCT GTCCTTCAAC CTCATCCAGA CAGAACGACA GAACCACGCC 
    CGGGAGCTGG TCCAGGGGCT GAGCCTGAGT GAGTGGGATG GCATCGTCAC GGTCTCGGGA
    GACGGGCTGC TCCATGAGGT GCTGAACGGG CTCCTAGATC GCCCTGACTG GGAGGAAGCT
    GTGAAGATGC CTGTGGGCAT CCTCCCCTGC GGCTCGGGCA ACGCGCTGGC CGGAGCAGTG
    AACCAGCACG GGGGATTTGA GCCAGCCCTG GGCCTCGACC TGTTGCTCAA CTGCTCACTG
    TTGCTGTGCC GGGGTGGTGG CCACCCACTG GACCTGCTCT CCGTGACGCT GGCCTCGGGC
    TCCCGCTGTT TCTCCTTCCT GTCTGTGGCC TGGGGCTTCG TGTCAGATGT GGATATCCAG
    AGCGAGCGCT TCAGGGCCTT GGGCAGTGCC CGCTTCACAC TGGGCACGGT GCTGGGCCTC
    GCCACACTGC ACACCTACCG CGGACGCCTC TCCTACCTCC CCGCCACTGT GGAACCTGCC
    TCGCCCACCC CTGCCCATAG CCTGCCTCGT GCCAAGTCGG AGCTGACCCT AACCCCAGAC
    CCAGCCCCGC CCATGGCCCA CTCACCCCTG CATCGTTCTG TGTCTGACCT GCCTCTTCCC
    CTGCCCCAGC CTGCCCTGGC CTCTCCTGGC TCGCCAGAAC CCCTGCCCAT CCTGTCCCTC
    AACGGTGGGG GCCCAGAGCT GGCTGGGGAC TGGGGTGGGG CTGGGGATGC TCCGCTGTCC
    CCGGACCCAC TGCTGTCTTC ACCTCCTGGC TCTCCCAAGG CAGCTCTACA CTCACCCGTC
    TCCGAAGGGG CCCCCGTAAT TCCCCCATCC TCTGGGCTCC CACTTCCCAC CCCTGATGCC
    CGGGTAGGGG CCTCCACCTG CGGCCCGCCC GACCACCTGC TGCCTCCGCT GGGCACCCCG
    CTGCCCCCAG ACTGGGTGAC GCTGGAGGGG GACTTTGTGC TCATGTTGGC CATCTCGCCC
    AGCCACCTAG GCGCTGACCT GGTGGCAGCT CCGCATGCGC GCTTCGACGA CGGCCTGGTG
    CACCTGTGCT GGGTGCGTAG CGGCATCTCG CGGGCTGCGC TGCTGCGCCT TTTCTTGGCC
    ATGGAGCGTG GTAGCCACTT CAGCCTGGGC TGTCCGCAGC TGGGCTACGC CGCGGCCCGT
    GCCTTCCGCC TAGAGCCGCT CACACCACGC GGCGTGCTCA CAGTGGACGG GGAGCAGGTG
    GAGTATGGGC CGCTACAGGC ACAGATGCAC CCTGGCATCG GTACACTGCT CACTGGGCCT
    CCTGGCTGCC CGGGGCGGGA GCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16817
    Clone ID Related Accession (Same CDS sequence) NM_001204158.2 , NM_001204158.3
    Accession Version NM_001204158.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-03-11
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BI224962.1, AK300541.1, AK122735.1 and DB367710.1. On Sep 1, 2011 this sequence version replaced NM_001204158.1. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300541.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGG CCCTGCACAT ACAGCGGCTG 
    CGCCCCAAAC CTGAAGCCAG GCCCCGGGGT GGCCTGGTCC CGTTGGCCGA GGTCTCAGGC
    TGCTGCACCC TGCGAAGCCG CAGCCCCTCA GACTCAGCGG CCTACTTCTG CATCTACACC
    TACCCTCGGG GCCGGCGCGG GGCCCGGCGC AGAGCCACTC GCACCTTCCG GGCAGATGGG
    GCCGCCACCT ACGAAGAGAA CCGTGCCGAG GCCCAGCGCT GGGCCACTGC CCTCACCTGT
    CTGCTCCGAG GACTGCCACT GCCCGGGGAT GGGGAGATCA CCCCTGACCT GCTACCTCGG
    CCGCCCCGGT TGCTTCTATT GGTCAATCCC TTTGGGGGTC GGGGCCTGGC CTGGCAGTGG
    TGTAAGAACC ACGTGCTTCC CATGATCTCT GAAGCTGGGC TGTCCTTCAA CCTCATCCAG
    ACAGAACGAC AGAACCACGC CCGGGAGCTG GTCCAGGGGC TGAGCCTGAG TGAGTGGGAT
    GGCATCGTCA CGGTCTCGGG AGACGGGCTG CTCCATGAGG TGCTGAACGG GCTCCTAGAT
    CGCCCTGACT GGGAGGAAGC TGTGAAGATG CCTGTGGGCA TCCTCCCCTG CGGCTCGGGC
    AACGCGCTGG CCGGAGCAGT GAACCAGCAC GGGGGATTTG AGCCAGCCCT GGGCCTCGAC
    CTGTTGCTCA ACTGCTCACT GTTGCTGTGC CGGGGTGGTG GCCACCCACT GGACCTGCTC
    TCCGTGACGC TGGCCTCGGG CTCCCGCTGT TTCTCCTTCC TGTCTGTGGC CTGGGGCTTC
    GTGTCAGATG TGGATATCCA GAGCGAGCGC TTCAGGGCCT TGGGCAGTGC CCGCTTCACA
    CTGGGCACGG TGCTGGGCCT CGCCACACTG CACACCTACC GCGGACGCCT CTCCTACCTC
    CCCGCCACTG TGGAACCTGC CTCGCCCACC CCTGCCCATA GCCTGCCTCG TGCCAAGTCG
    GAGCTGACCC TAACCCCAGA CCCAGCCCCG CCCATGGCCC ACTCACCCCT GCATCGTTCT
    GTGTCTGACC TGCCTCTTCC CCTGCCCCAG CCTGCCCTGG CCTCTCCTGG CTCGCCAGAA
    CCCCTGCCCA TCCTGTCCCT CAACGGTGGG GGCCCAGAGC TGGCTGGGGA CTGGGGTGGG
    GCTGGGGATG CTCCGCTGTC CCCGGACCCA CTGCTGTCTT CACCTCCTGG CTCTCCCAAG
    GCAGCTCTAC ACTCACCCGT CTCCGAAGGG GCCCCCGTAA TTCCCCCATC CTCTGGGCTC
    CCACTTCCCA CCCCTGATGC CCGGGTAGGG GCCTCCACCT GCGGCCCGCC CGACCACCTG
    CTGCCTCCGC TGGGCACCCC GCTGCCCCCA GACTGGGTGA CGCTGGAGGG GGACTTTGTG
    CTCATGTTGG CCATCTCGCC CAGCCACCTA GGCGCTGACC TGGTGGCAGC TCCGCATGCG
    CGCTTCGACG ACGGCCTGGT GCACCTGTGC TGGGTGCGTA GCGGCATCTC GCGGGCTGCG
    CTGCTGCGCC TTTTCTTGGC CATGGAGCGT GGTAGCCACT TCAGCCTGGG CTGTCCGCAG
    CTGGGCTACG CCGCGGCCCG TGCCTTCCGC CTAGAGCCGC TCACACCACG CGGCGTGCTC
    ACAGTGGACG GGGAGCAGGT GGAGTATGGG CCGCTACAGG CACAGATGCA CCCTGGCATC
    GGTACACTGC TCACTGGGCC TCCTGGCTGC CCGGGGCGGG AGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191087.1
    CDS367..2154
    Translation

    Target ORF information:

    RefSeq Version NM_001204158.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204158.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGG CCCTGCACAT ACAGCGGCTG 
    CGCCCCAAAC CTGAAGCCAG GCCCCGGGGT GGCCTGGTCC CGTTGGCCGA GGTCTCAGGC
    TGCTGCACCC TGCGAAGCCG CAGCCCCTCA GACTCAGCGG CCTACTTCTG CATCTACACC
    TACCCTCGGG GCCGGCGCGG GGCCCGGCGC AGAGCCACTC GCACCTTCCG GGCAGATGGG
    GCCGCCACCT ACGAAGAGAA CCGTGCCGAG GCCCAGCGCT GGGCCACTGC CCTCACCTGT
    CTGCTCCGAG GACTGCCACT GCCCGGGGAT GGGGAGATCA CCCCTGACCT GCTACCTCGG
    CCGCCCCGGT TGCTTCTATT GGTCAATCCC TTTGGGGGTC GGGGCCTGGC CTGGCAGTGG
    TGTAAGAACC ACGTGCTTCC CATGATCTCT GAAGCTGGGC TGTCCTTCAA CCTCATCCAG
    ACAGAACGAC AGAACCACGC CCGGGAGCTG GTCCAGGGGC TGAGCCTGAG TGAGTGGGAT
    GGCATCGTCA CGGTCTCGGG AGACGGGCTG CTCCATGAGG TGCTGAACGG GCTCCTAGAT
    CGCCCTGACT GGGAGGAAGC TGTGAAGATG CCTGTGGGCA TCCTCCCCTG CGGCTCGGGC
    AACGCGCTGG CCGGAGCAGT GAACCAGCAC GGGGGATTTG AGCCAGCCCT GGGCCTCGAC
    CTGTTGCTCA ACTGCTCACT GTTGCTGTGC CGGGGTGGTG GCCACCCACT GGACCTGCTC
    TCCGTGACGC TGGCCTCGGG CTCCCGCTGT TTCTCCTTCC TGTCTGTGGC CTGGGGCTTC
    GTGTCAGATG TGGATATCCA GAGCGAGCGC TTCAGGGCCT TGGGCAGTGC CCGCTTCACA
    CTGGGCACGG TGCTGGGCCT CGCCACACTG CACACCTACC GCGGACGCCT CTCCTACCTC
    CCCGCCACTG TGGAACCTGC CTCGCCCACC CCTGCCCATA GCCTGCCTCG TGCCAAGTCG
    GAGCTGACCC TAACCCCAGA CCCAGCCCCG CCCATGGCCC ACTCACCCCT GCATCGTTCT
    GTGTCTGACC TGCCTCTTCC CCTGCCCCAG CCTGCCCTGG CCTCTCCTGG CTCGCCAGAA
    CCCCTGCCCA TCCTGTCCCT CAACGGTGGG GGCCCAGAGC TGGCTGGGGA CTGGGGTGGG
    GCTGGGGATG CTCCGCTGTC CCCGGACCCA CTGCTGTCTT CACCTCCTGG CTCTCCCAAG
    GCAGCTCTAC ACTCACCCGT CTCCGAAGGG GCCCCCGTAA TTCCCCCATC CTCTGGGCTC
    CCACTTCCCA CCCCTGATGC CCGGGTAGGG GCCTCCACCT GCGGCCCGCC CGACCACCTG
    CTGCCTCCGC TGGGCACCCC GCTGCCCCCA GACTGGGTGA CGCTGGAGGG GGACTTTGTG
    CTCATGTTGG CCATCTCGCC CAGCCACCTA GGCGCTGACC TGGTGGCAGC TCCGCATGCG
    CGCTTCGACG ACGGCCTGGT GCACCTGTGC TGGGTGCGTA GCGGCATCTC GCGGGCTGCG
    CTGCTGCGCC TTTTCTTGGC CATGGAGCGT GGTAGCCACT TCAGCCTGGG CTGTCCGCAG
    CTGGGCTACG CCGCGGCCCG TGCCTTCCGC CTAGAGCCGC TCACACCACG CGGCGTGCTC
    ACAGTGGACG GGGAGCAGGT GGAGTATGGG CCGCTACAGG CACAGATGCA CCCTGGCATC
    GGTACACTGC TCACTGGGCC TCCTGGCTGC CCGGGGCGGG AGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu16817
    Clone ID Related Accession (Same CDS sequence) NM_001204158.2 , NM_001204158.3
    Accession Version NM_001204158.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1788bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-03
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK300541.1, AK122735.1 and DB367710.1. On May 31, 2019 this sequence version replaced NM_001204158.2. Transcript Variant: This variant (2) uses an alternate in-frame splice site in the coding region, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK300541.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142680, SAMEA2144120 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGG CCCTGCACAT ACAGCGGCTG 
    CGCCCCAAAC CTGAAGCCAG GCCCCGGGGT GGCCTGGTCC CGTTGGCCGA GGTCTCAGGC
    TGCTGCACCC TGCGAAGCCG CAGCCCCTCA GACTCAGCGG CCTACTTCTG CATCTACACC
    TACCCTCGGG GCCGGCGCGG GGCCCGGCGC AGAGCCACTC GCACCTTCCG GGCAGATGGG
    GCCGCCACCT ACGAAGAGAA CCGTGCCGAG GCCCAGCGCT GGGCCACTGC CCTCACCTGT
    CTGCTCCGAG GACTGCCACT GCCCGGGGAT GGGGAGATCA CCCCTGACCT GCTACCTCGG
    CCGCCCCGGT TGCTTCTATT GGTCAATCCC TTTGGGGGTC GGGGCCTGGC CTGGCAGTGG
    TGTAAGAACC ACGTGCTTCC CATGATCTCT GAAGCTGGGC TGTCCTTCAA CCTCATCCAG
    ACAGAACGAC AGAACCACGC CCGGGAGCTG GTCCAGGGGC TGAGCCTGAG TGAGTGGGAT
    GGCATCGTCA CGGTCTCGGG AGACGGGCTG CTCCATGAGG TGCTGAACGG GCTCCTAGAT
    CGCCCTGACT GGGAGGAAGC TGTGAAGATG CCTGTGGGCA TCCTCCCCTG CGGCTCGGGC
    AACGCGCTGG CCGGAGCAGT GAACCAGCAC GGGGGATTTG AGCCAGCCCT GGGCCTCGAC
    CTGTTGCTCA ACTGCTCACT GTTGCTGTGC CGGGGTGGTG GCCACCCACT GGACCTGCTC
    TCCGTGACGC TGGCCTCGGG CTCCCGCTGT TTCTCCTTCC TGTCTGTGGC CTGGGGCTTC
    GTGTCAGATG TGGATATCCA GAGCGAGCGC TTCAGGGCCT TGGGCAGTGC CCGCTTCACA
    CTGGGCACGG TGCTGGGCCT CGCCACACTG CACACCTACC GCGGACGCCT CTCCTACCTC
    CCCGCCACTG TGGAACCTGC CTCGCCCACC CCTGCCCATA GCCTGCCTCG TGCCAAGTCG
    GAGCTGACCC TAACCCCAGA CCCAGCCCCG CCCATGGCCC ACTCACCCCT GCATCGTTCT
    GTGTCTGACC TGCCTCTTCC CCTGCCCCAG CCTGCCCTGG CCTCTCCTGG CTCGCCAGAA
    CCCCTGCCCA TCCTGTCCCT CAACGGTGGG GGCCCAGAGC TGGCTGGGGA CTGGGGTGGG
    GCTGGGGATG CTCCGCTGTC CCCGGACCCA CTGCTGTCTT CACCTCCTGG CTCTCCCAAG
    GCAGCTCTAC ACTCACCCGT CTCCGAAGGG GCCCCCGTAA TTCCCCCATC CTCTGGGCTC
    CCACTTCCCA CCCCTGATGC CCGGGTAGGG GCCTCCACCT GCGGCCCGCC CGACCACCTG
    CTGCCTCCGC TGGGCACCCC GCTGCCCCCA GACTGGGTGA CGCTGGAGGG GGACTTTGTG
    CTCATGTTGG CCATCTCGCC CAGCCACCTA GGCGCTGACC TGGTGGCAGC TCCGCATGCG
    CGCTTCGACG ACGGCCTGGT GCACCTGTGC TGGGTGCGTA GCGGCATCTC GCGGGCTGCG
    CTGCTGCGCC TTTTCTTGGC CATGGAGCGT GGTAGCCACT TCAGCCTGGG CTGTCCGCAG
    CTGGGCTACG CCGCGGCCCG TGCCTTCCGC CTAGAGCCGC TCACACCACG CGGCGTGCTC
    ACAGTGGACG GGGAGCAGGT GGAGTATGGG CCGCTACAGG CACAGATGCA CCCTGGCATC
    GGTACACTGC TCACTGGGCC TCCTGGCTGC CCGGGGCGGG AGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001191087.1
    CDS152..1939
    Translation

    Target ORF information:

    RefSeq Version NM_001204158.3
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001204158.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGG CCCTGCACAT ACAGCGGCTG 
    CGCCCCAAAC CTGAAGCCAG GCCCCGGGGT GGCCTGGTCC CGTTGGCCGA GGTCTCAGGC
    TGCTGCACCC TGCGAAGCCG CAGCCCCTCA GACTCAGCGG CCTACTTCTG CATCTACACC
    TACCCTCGGG GCCGGCGCGG GGCCCGGCGC AGAGCCACTC GCACCTTCCG GGCAGATGGG
    GCCGCCACCT ACGAAGAGAA CCGTGCCGAG GCCCAGCGCT GGGCCACTGC CCTCACCTGT
    CTGCTCCGAG GACTGCCACT GCCCGGGGAT GGGGAGATCA CCCCTGACCT GCTACCTCGG
    CCGCCCCGGT TGCTTCTATT GGTCAATCCC TTTGGGGGTC GGGGCCTGGC CTGGCAGTGG
    TGTAAGAACC ACGTGCTTCC CATGATCTCT GAAGCTGGGC TGTCCTTCAA CCTCATCCAG
    ACAGAACGAC AGAACCACGC CCGGGAGCTG GTCCAGGGGC TGAGCCTGAG TGAGTGGGAT
    GGCATCGTCA CGGTCTCGGG AGACGGGCTG CTCCATGAGG TGCTGAACGG GCTCCTAGAT
    CGCCCTGACT GGGAGGAAGC TGTGAAGATG CCTGTGGGCA TCCTCCCCTG CGGCTCGGGC
    AACGCGCTGG CCGGAGCAGT GAACCAGCAC GGGGGATTTG AGCCAGCCCT GGGCCTCGAC
    CTGTTGCTCA ACTGCTCACT GTTGCTGTGC CGGGGTGGTG GCCACCCACT GGACCTGCTC
    TCCGTGACGC TGGCCTCGGG CTCCCGCTGT TTCTCCTTCC TGTCTGTGGC CTGGGGCTTC
    GTGTCAGATG TGGATATCCA GAGCGAGCGC TTCAGGGCCT TGGGCAGTGC CCGCTTCACA
    CTGGGCACGG TGCTGGGCCT CGCCACACTG CACACCTACC GCGGACGCCT CTCCTACCTC
    CCCGCCACTG TGGAACCTGC CTCGCCCACC CCTGCCCATA GCCTGCCTCG TGCCAAGTCG
    GAGCTGACCC TAACCCCAGA CCCAGCCCCG CCCATGGCCC ACTCACCCCT GCATCGTTCT
    GTGTCTGACC TGCCTCTTCC CCTGCCCCAG CCTGCCCTGG CCTCTCCTGG CTCGCCAGAA
    CCCCTGCCCA TCCTGTCCCT CAACGGTGGG GGCCCAGAGC TGGCTGGGGA CTGGGGTGGG
    GCTGGGGATG CTCCGCTGTC CCCGGACCCA CTGCTGTCTT CACCTCCTGG CTCTCCCAAG
    GCAGCTCTAC ACTCACCCGT CTCCGAAGGG GCCCCCGTAA TTCCCCCATC CTCTGGGCTC
    CCACTTCCCA CCCCTGATGC CCGGGTAGGG GCCTCCACCT GCGGCCCGCC CGACCACCTG
    CTGCCTCCGC TGGGCACCCC GCTGCCCCCA GACTGGGTGA CGCTGGAGGG GGACTTTGTG
    CTCATGTTGG CCATCTCGCC CAGCCACCTA GGCGCTGACC TGGTGGCAGC TCCGCATGCG
    CGCTTCGACG ACGGCCTGGT GCACCTGTGC TGGGTGCGTA GCGGCATCTC GCGGGCTGCG
    CTGCTGCGCC TTTTCTTGGC CATGGAGCGT GGTAGCCACT TCAGCCTGGG CTGTCCGCAG
    CTGGGCTACG CCGCGGCCCG TGCCTTCCGC CTAGAGCCGC TCACACCACG CGGCGTGCTC
    ACAGTGGACG GGGAGCAGGT GGAGTATGGG CCGCTACAGG CACAGATGCA CCCTGGCATC
    GGTACACTGC TCACTGGGCC TCCTGGCTGC CCGGGGCGGG AGCCCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70093
    Clone ID Related Accession (Same CDS sequence) XM_017027010.2 , XM_017027010.1
    Accession Version XM_017027010.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_017027010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGCTGTATCC CGAGCGCCCA GAACTCTGCC
    TGGCATGGGA GGCACTCAGT GAATTGTAGG GAGCAAATGA AAGATGACCA GGAACCTGGC
    CCCGTAAGGA GTCGCCTGGA GGTGGCCCCA CGGCTGTGGT GGGCCTGGGC CATGGCCTTC
    GTGGTCTCAT TGCCAGCTGC TTTCCTACCT GTCTCTTTCC CCAACCCCTG TTTGCTCCTT
    CCTTCTGTGT GTCCGTCCAT CTCCGGCTGT GAAGATTTGA GCCAGCCCTG GGCCTCGACC
    TGTTGCTCAA CTGCTCACTG TTGCTGTGCC GGGGTGGTGG CCACCCACTG GACCTGCTCT
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016882499.1
    CDS249..1454
    Translation

    Target ORF information:

    RefSeq Version XM_017027010.2
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027010.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGCTGTATCC CGAGCGCCCA GAACTCTGCC
    TGGCATGGGA GGCACTCAGT GAATTGTAGG GAGCAAATGA AAGATGACCA GGAACCTGGC
    CCCGTAAGGA GTCGCCTGGA GGTGGCCCCA CGGCTGTGGT GGGCCTGGGC CATGGCCTTC
    GTGGTCTCAT TGCCAGCTGC TTTCCTACCT GTCTCTTTCC CCAACCCCTG TTTGCTCCTT
    CCTTCTGTGT GTCCGTCCAT CTCCGGCTGT GAAGATTTGA GCCAGCCCTG GGCCTCGACC
    TGTTGCTCAA CTGCTCACTG TTGCTGTGCC GGGGTGGTGG CCACCCACTG GACCTGCTCT
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu70093
    Clone ID Related Accession (Same CDS sequence) XM_017027010.2 , XM_017027010.1
    Accession Version XM_017027010.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1206bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGCTGTATCC CGAGCGCCCA GAACTCTGCC
    TGGCATGGGA GGCACTCAGT GAATTGTAGG GAGCAAATGA AAGATGACCA GGAACCTGGC
    CCCGTAAGGA GTCGCCTGGA GGTGGCCCCA CGGCTGTGGT GGGCCTGGGC CATGGCCTTC
    GTGGTCTCAT TGCCAGCTGC TTTCCTACCT GTCTCTTTCC CCAACCCCTG TTTGCTCCTT
    CCTTCTGTGT GTCCGTCCAT CTCCGGCTGT GAAGATTTGA GCCAGCCCTG GGCCTCGACC
    TGTTGCTCAA CTGCTCACTG TTGCTGTGCC GGGGTGGTGG CCACCCACTG GACCTGCTCT
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016882499.1
    CDS257..1462
    Translation

    Target ORF information:

    RefSeq Version XM_017027010.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027010.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    ATGAATGGAC ACCTTGAAGC AGAGGAGCAG CAGGACCAGA GGCCAGACCA GGAGCTGACC 
    GGGAGCTGGG GCCACGGGCC TAGGAGCACC CTGGTCAGGG CTAAGGCCAT GGCCCCGCCC
    CCACCGCCAC TGGCTGCCAG CACCCCGCTC CTCCATGGCG AGTTTGGCTC CTACCCAGCC
    CGAGGCCCAC GCTTTGCCCT CACCCTTACA TCGCAGGCCC TGCACATACA GCGGCTGCGC
    CCCAAACCTG AAGCCAGGCC CCGGGGTGGC CTGGTCCCGT TGGCCGAGGT CTCAGGCTGC
    TGCACCCTGC GAAGCCGCAG CCCCTCAGAC TCAGCGGCCT ACTTCTGCAT CTACACCTAC
    CCTCGGGGCC GGCGCGGGGC CCGGCGCAGA GCCACTCGCA CCTTCCGGGC AGATGGGGCC
    GCCACCTACG AAGAGAACCG TGCCGAGGCC CAGCGCTGGG CCACTGCCCT CACCTGTCTG
    CTCCGAGGAC TGCCACTGCC CGGGGATGGG GAGATCACCC CTGACCTGCT ACCTCGGCCG
    CCCCGGTTGC TTCTATTGGT CAATCCCTTT GGGGGTCGGG GCCTGGCCTG GCAGTGGTGT
    AAGAACCACG TGCTTCCCAT GATCTCTGAA GCTGGGCTGT CCTTCAACCT CATCCAGACA
    GAACGACAGA ACCACGCCCG GGAGCTGGTC CAGGGGCTGA GCCTGAGTGA GTGGGATGGC
    ATCGTCACGG TCTCGGGAGA CGGGCTGCTC CATGAGGTGC TGAACGGGCT CCTAGATCGC
    CCTGACTGGG AGGAAGCTGT GAAGATGCCT GTGGGCATCC TCCCCTGCGG CTCGGGCAAC
    GCGCTGGCCG GAGCAGTGAA CCAGCACGGG GGCTGTATCC CGAGCGCCCA GAACTCTGCC
    TGGCATGGGA GGCACTCAGT GAATTGTAGG GAGCAAATGA AAGATGACCA GGAACCTGGC
    CCCGTAAGGA GTCGCCTGGA GGTGGCCCCA CGGCTGTGGT GGGCCTGGGC CATGGCCTTC
    GTGGTCTCAT TGCCAGCTGC TTTCCTACCT GTCTCTTTCC CCAACCCCTG TTTGCTCCTT
    CCTTCTGTGT GTCCGTCCAT CTCCGGCTGT GAAGATTTGA GCCAGCCCTG GGCCTCGACC
    TGTTGCTCAA CTGCTCACTG TTGCTGTGCC GGGGTGGTGG CCACCCACTG GACCTGCTCT
    CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu88577
    Clone ID Related Accession (Same CDS sequence) XM_017027008.1
    Accession Version XM_017027008.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2151bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product sphingosine kinase 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATTGGAT GTTTGCATGC CCGGGTCTCC GGCCCCCTCT GGGATGCTGG CCTCTGTCCC 
    GCATCCTCAA GGTCTGCCCA CACCTGTCTG AGCCTGTCTG TCTCTGATGC TCCTGTCTCA
    CCTGCCACTG CCCCTCATTG TCTCCTCCTG TCCACAGCCC CTGCCCCTCC CTGCCCCTGC
    CATGGGGTCC TGAATTCTCA CCCCTTCTCT CCTCCCTTCC CACAGAGGCC AGACCAGGAG
    CTGACCGGGA GCTGGGGCCA CGGGCCTAGG AGCACCCTGG TCAGGGCTAA GGCCATGGCC
    CCGCCCCCAC CGCCACTGGC TGCCAGCACC CCGCTCCTCC ATGGCGAGTT TGGCTCCTAC
    CCAGCCCGAG GCCCACGCTT TGCCCTCACC CTTACATCGC AGGCCCTGCA CATACAGCGG
    CTGCGCCCCA AACCTGAAGC CAGGCCCCGG GGTGGCCTGG TCCCGTTGGC CGAGGTCTCA
    GGCTGCTGCA CCCTGCGAAG CCGCAGCCCC TCAGACTCAG CGGCCTACTT CTGCATCTAC
    ACCTACCCTC GGGGCCGGCG CGGGGCCCGG CGCAGAGCCA CTCGCACCTT CCGGGCAGAT
    GGGGCCGCCA CCTACGAAGA GAACCGTGCC GAGGCCCAGC GCTGGGCCAC TGCCCTCACC
    TGTCTGCTCC GAGGACTGCC ACTGCCCGGG GATGGGGAGA TCACCCCTGA CCTGCTACCT
    CGGCCGCCCC GGTTGCTTCT ATTGGTCAAT CCCTTTGGGG GTCGGGGCCT GGCCTGGCAG
    TGGTGTAAGA ACCACGTGCT TCCCATGATC TCTGAAGCTG GGCTGTCCTT CAACCTCATC
    CAGACAGAAC GACAGAACCA CGCCCGGGAG CTGGTCCAGG GGCTGAGCCT GAGTGAGTGG
    GATGGCATCG TCACGGTCTC GGGAGACGGG CTGCTCCATG AGGTGCTGAA CGGGCTCCTA
    GATCGCCCTG ACTGGGAGGA AGCTGTGAAG ATGCCTGTGG GCATCCTCCC CTGCGGCTCG
    GGCAACGCGC TGGCCGGAGC AGTGAACCAG CACGGGGGAT TTGAGCCAGC CCTGGGCCTC
    GACCTGTTGC TCAACTGCTC ACTGTTGCTG TGCCGGGGTG GTGGCCACCC ACTGGACCTG
    CTCTCCGTGA CGCTGGCCTC GGGCTCCCGC TGTTTCTCCT TCCTGTCTGT GGCCTGGGGC
    TTCGTGTCAG ATGTGGATAT CCAGAGCGAG CGCTTCAGGG CCTTGGGCAG TGCCCGCTTC
    ACACTGGGCA CGGTGCTGGG CCTCGCCACA CTGCACACCT ACCGCGGACG CCTCTCCTAC
    CTCCCCGCCA CTGTGGAACC TGCCTCGCCC ACCCCTGCCC ATAGCCTGCC TCGTGCCAAG
    TCGGAGCTGA CCCTAACCCC AGACCCAGCC CCGCCCATGG CCCACTCACC CCTGCATCGT
    TCTGTGTCTG ACCTGCCTCT TCCCCTGCCC CAGCCTGCCC TGGCCTCTCC TGGCTCGCCA
    GAACCCCTGC CCATCCTGTC CCTCAACGGT GGGGGCCCAG AGCTGGCTGG GGACTGGGGT
    GGGGCTGGGG ATGCTCCGCT GTCCCCGGAC CCACTGCTGT CTTCACCTCC TGGCTCTCCC
    AAGGCAGCTC TACACTCACC CGTCTCCGAA GGGGCCCCCG TAATTCCCCC ATCCTCTGGG
    CTCCCACTTC CCACCCCTGA TGCCCGGGTA GGGGCCTCCA CCTGCGGCCC GCCCGACCAC
    CTGCTGCCTC CGCTGGGCAC CCCGCTGCCC CCAGACTGGG TGACGCTGGA GGGGGACTTT
    GTGCTCATGT TGGCCATCTC GCCCAGCCAC CTAGGCGCTG ACCTGGTGGC AGCTCCGCAT
    GCGCGCTTCG ACGACGGCCT GGTGCACCTG TGCTGGGTGC GTAGCGGCAT CTCGCGGGCT
    GCGCTGCTGC GCCTTTTCTT GGCCATGGAG CGTGGTAGCC ACTTCAGCCT GGGCTGTCCG
    CAGCTGGGCT ACGCCGCGGC CCGTGCCTTC CGCCTAGAGC CGCTCACACC ACGCGGCGTG
    CTCACAGTGG ACGGGGAGCA GGTGGAGTAT GGGCCGCTAC AGGCACAGAT GCACCCTGGC
    ATCGGTACAC TGCTCACTGG GCCTCCTGGC TGCCCGGGGC GGGAGCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016882497.1
    CDS587..2737
    Translation

    Target ORF information:

    RefSeq Version XM_017027008.1
    Organism Homo sapiens(human)
    Definition Homo sapiens sphingosine kinase 2 (SPHK2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017027008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    ATGATTGGAT GTTTGCATGC CCGGGTCTCC GGCCCCCTCT GGGATGCTGG CCTCTGTCCC 
    GCATCCTCAA GGTCTGCCCA CACCTGTCTG AGCCTGTCTG TCTCTGATGC TCCTGTCTCA
    CCTGCCACTG CCCCTCATTG TCTCCTCCTG TCCACAGCCC CTGCCCCTCC CTGCCCCTGC
    CATGGGGTCC TGAATTCTCA CCCCTTCTCT CCTCCCTTCC CACAGAGGCC AGACCAGGAG
    CTGACCGGGA GCTGGGGCCA CGGGCCTAGG AGCACCCTGG TCAGGGCTAA GGCCATGGCC
    CCGCCCCCAC CGCCACTGGC TGCCAGCACC CCGCTCCTCC ATGGCGAGTT TGGCTCCTAC
    CCAGCCCGAG GCCCACGCTT TGCCCTCACC CTTACATCGC AGGCCCTGCA CATACAGCGG
    CTGCGCCCCA AACCTGAAGC CAGGCCCCGG GGTGGCCTGG TCCCGTTGGC CGAGGTCTCA
    GGCTGCTGCA CCCTGCGAAG CCGCAGCCCC TCAGACTCAG CGGCCTACTT CTGCATCTAC
    ACCTACCCTC GGGGCCGGCG CGGGGCCCGG CGCAGAGCCA CTCGCACCTT CCGGGCAGAT
    GGGGCCGCCA CCTACGAAGA GAACCGTGCC GAGGCCCAGC GCTGGGCCAC TGCCCTCACC
    TGTCTGCTCC GAGGACTGCC ACTGCCCGGG GATGGGGAGA TCACCCCTGA CCTGCTACCT
    CGGCCGCCCC GGTTGCTTCT ATTGGTCAAT CCCTTTGGGG GTCGGGGCCT GGCCTGGCAG
    TGGTGTAAGA ACCACGTGCT TCCCATGATC TCTGAAGCTG GGCTGTCCTT CAACCTCATC
    CAGACAGAAC GACAGAACCA CGCCCGGGAG CTGGTCCAGG GGCTGAGCCT GAGTGAGTGG
    GATGGCATCG TCACGGTCTC GGGAGACGGG CTGCTCCATG AGGTGCTGAA CGGGCTCCTA
    GATCGCCCTG ACTGGGAGGA AGCTGTGAAG ATGCCTGTGG GCATCCTCCC CTGCGGCTCG
    GGCAACGCGC TGGCCGGAGC AGTGAACCAG CACGGGGGAT TTGAGCCAGC CCTGGGCCTC
    GACCTGTTGC TCAACTGCTC ACTGTTGCTG TGCCGGGGTG GTGGCCACCC ACTGGACCTG
    CTCTCCGTGA CGCTGGCCTC GGGCTCCCGC TGTTTCTCCT TCCTGTCTGT GGCCTGGGGC
    TTCGTGTCAG ATGTGGATAT CCAGAGCGAG CGCTTCAGGG CCTTGGGCAG TGCCCGCTTC
    ACACTGGGCA CGGTGCTGGG CCTCGCCACA CTGCACACCT ACCGCGGACG CCTCTCCTAC
    CTCCCCGCCA CTGTGGAACC TGCCTCGCCC ACCCCTGCCC ATAGCCTGCC TCGTGCCAAG
    TCGGAGCTGA CCCTAACCCC AGACCCAGCC CCGCCCATGG CCCACTCACC CCTGCATCGT
    TCTGTGTCTG ACCTGCCTCT TCCCCTGCCC CAGCCTGCCC TGGCCTCTCC TGGCTCGCCA
    GAACCCCTGC CCATCCTGTC CCTCAACGGT GGGGGCCCAG AGCTGGCTGG GGACTGGGGT
    GGGGCTGGGG ATGCTCCGCT GTCCCCGGAC CCACTGCTGT CTTCACCTCC TGGCTCTCCC
    AAGGCAGCTC TACACTCACC CGTCTCCGAA GGGGCCCCCG TAATTCCCCC ATCCTCTGGG
    CTCCCACTTC CCACCCCTGA TGCCCGGGTA GGGGCCTCCA CCTGCGGCCC GCCCGACCAC
    CTGCTGCCTC CGCTGGGCAC CCCGCTGCCC CCAGACTGGG TGACGCTGGA GGGGGACTTT
    GTGCTCATGT TGGCCATCTC GCCCAGCCAC CTAGGCGCTG ACCTGGTGGC AGCTCCGCAT
    GCGCGCTTCG ACGACGGCCT GGTGCACCTG TGCTGGGTGC GTAGCGGCAT CTCGCGGGCT
    GCGCTGCTGC GCCTTTTCTT GGCCATGGAG CGTGGTAGCC ACTTCAGCCT GGGCTGTCCG
    CAGCTGGGCT ACGCCGCGGC CCGTGCCTTC CGCCTAGAGC CGCTCACACC ACGCGGCGTG
    CTCACAGTGG ACGGGGAGCA GGTGGAGTAT GGGCCGCTAC AGGCACAGAT GCACCCTGGC
    ATCGGTACAC TGCTCACTGG GCCTCCTGGC TGCCCGGGGC GGGAGCCCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.