TWNK cDNA ORF clone, Homo sapiens(human)

The following TWNK gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TWNK cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu26782 NM_021830.5
Latest version!
Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$344.50-$482.30
$689.00
OHu26432 NM_001368275.1
Latest version!
Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu26432 NM_001163813.2
Latest version!
Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu26432 XM_011539975.2
Latest version!
Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu26432 XM_017016437.1
Latest version!
Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu22794 NM_001163814.2
Latest version!
Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu26964 NM_001163812.2
Latest version!
Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
View Old Accession Versions >>
OHu26432 NM_001163813.1 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 5-7 $139.30
$199.00
OHu22794 NM_001163814.1 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $69.30
$99.00
OHu26964 NM_001163812.1 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
OHu26782 NM_021830.4 Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$344.50-$482.30
$689.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu26782
    Clone ID Related Accession (Same CDS sequence) NM_021830.5 , NM_021830.4
    Accession Version NM_021830.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product twinkle protein, mitochondrial isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB090322.1, AF292004.1, AK025485.1 and AL133215.17. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_021830.4. Transcript Variant: This variant (1) encodes the longest isoform (A; also known as Twinkle). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.153796.1, SRR1660807.228176.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta MANE Ensembl match :: ENST00000311916.8/ ENSP00000309595.2 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC
    AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC
    CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA
    CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC
    AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT
    CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA
    GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT
    GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT
    GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG
    CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA
    CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG
    GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC
    CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG
    TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG
    TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC
    CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG
    CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA
    CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT
    ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA
    AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG
    TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC
    GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC
    CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA
    ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC
    ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG
    GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG
    AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGCAAGC
    CAGGAAGCAG ACAATGTTCT GATCCTGCAG GACAGGAAGC TGGTAACCGG GCCAGGGAAA
    CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGATG TAGGTGTCTT CCCGCTTGAG
    TTCAACAAGA ACTCCCTCAC CTTCTCCATT CCACCAAAGA ACAAGGCCCG GCTCAAGAAG
    ATCAAGGATG ACACTGGACC AGTGGCCAAA AAGCCCTCTT CTGGCAAAAA GGGGGCTACG
    ACACAGAACT CTGAGATTTG CTCAGGCCAG GCCCCCACTC CCGACCAGCC AGACACCTCC
    AAGCGTTCAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_068602.2
    CDS669..2723
    Translation

    Target ORF information:

    RefSeq Version NM_021830.5
    Organism Homo sapiens(human)
    Definition Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_021830.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC
    AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC
    CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA
    CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC
    AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT
    CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA
    GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT
    GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT
    GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG
    CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA
    CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG
    GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC
    CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG
    TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG
    TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC
    CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG
    CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA
    CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT
    ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA
    AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG
    TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC
    GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC
    CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA
    ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC
    ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG
    GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG
    AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGCAAGC
    CAGGAAGCAG ACAATGTTCT GATCCTGCAG GACAGGAAGC TGGTAACCGG GCCAGGGAAA
    CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGATG TAGGTGTCTT CCCGCTTGAG
    TTCAACAAGA ACTCCCTCAC CTTCTCCATT CCACCAAAGA ACAAGGCCCG GCTCAAGAAG
    ATCAAGGATG ACACTGGACC AGTGGCCAAA AAGCCCTCTT CTGGCAAAAA GGGGGCTACG
    ACACAGAACT CTGAGATTTG CTCAGGCCAG GCCCCCACTC CCGACCAGCC AGACACCTCC
    AAGCGTTCAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26782
    Clone ID Related Accession (Same CDS sequence) NM_021830.5 , NM_021830.4
    Accession Version NM_021830.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2055bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-10-22
    Organism Homo sapiens(human)
    Product twinkle protein, mitochondrial isoform A
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, DB090322.1, AF292004.1 and AK025485.1. This sequence is a reference standard in the RefSeqGene project. On Aug 5, 2009 this sequence version replaced NM_021830.3. Transcript Variant: This variant (1) encodes the longest isoform (A; also known as Twinkle). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC033762.1, SRR1660803.153796.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC
    AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC
    CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA
    CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC
    AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT
    CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA
    GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT
    GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT
    GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG
    CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA
    CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG
    GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC
    CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG
    TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG
    TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC
    CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG
    CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA
    CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT
    ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA
    AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG
    TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC
    GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC
    CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA
    ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC
    ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG
    GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG
    AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGCAAGC
    CAGGAAGCAG ACAATGTTCT GATCCTGCAG GACAGGAAGC TGGTAACCGG GCCAGGGAAA
    CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGATG TAGGTGTCTT CCCGCTTGAG
    TTCAACAAGA ACTCCCTCAC CTTCTCCATT CCACCAAAGA ACAAGGCCCG GCTCAAGAAG
    ATCAAGGATG ACACTGGACC AGTGGCCAAA AAGCCCTCTT CTGGCAAAAA GGGGGCTACG
    ACACAGAACT CTGAGATTTG CTCAGGCCAG GCCCCCACTC CCGACCAGCC AGACACCTCC
    AAGCGTTCAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_068602.2
    CDS676..2730
    Translation

    Target ORF information:

    RefSeq Version NM_021830.4
    Organism Homo sapiens(human)
    Definition Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_021830.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC
    AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC
    CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA
    CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC
    AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT
    CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA
    GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT
    GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT
    GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG
    CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA
    CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG
    GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC
    CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG
    TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG
    TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC
    CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG
    CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA
    CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT
    ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA
    AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG
    TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC
    GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC
    CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA
    ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC
    ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG
    GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG
    AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGCAAGC
    CAGGAAGCAG ACAATGTTCT GATCCTGCAG GACAGGAAGC TGGTAACCGG GCCAGGGAAA
    CGGTATCTGC AGGTGTCCAA GAACCGCTTT GATGGAGATG TAGGTGTCTT CCCGCTTGAG
    TTCAACAAGA ACTCCCTCAC CTTCTCCATT CCACCAAAGA ACAAGGCCCG GCTCAAGAAG
    ATCAAGGATG ACACTGGACC AGTGGCCAAA AAGCCCTCTT CTGGCAAAAA GGGGGCTACG
    ACACAGAACT CTGAGATTTG CTCAGGCCAG GCCCCCACTC CCGACCAGCC AGACACCTCC
    AAGCGTTCAA AGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26432
    Clone ID Related Accession (Same CDS sequence) NM_001368275.1 , XM_011539975.2 , NM_001163813.2 , XM_017016437.1 , NM_001163813.1
    Accession Version NM_001368275.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product twinkle protein, mitochondrial isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AL133215.17. On Feb 2, 2019 this sequence version replaced XM_011539975.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.431917.1, SRR1163657.209190.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA2146411 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
    GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
    GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
    AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
    GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
    GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001355204.1
    CDS129..821
    Translation

    Target ORF information:

    RefSeq Version NM_001368275.1
    Organism Homo sapiens(human)
    Definition Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001368275.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
    GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
    GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
    AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
    GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
    GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26432
    Clone ID Related Accession (Same CDS sequence) NM_001368275.1 , XM_011539975.2 , NM_001163813.2 , XM_017016437.1 , NM_001163813.1
    Accession Version NM_001163813.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product twinkle protein, mitochondrial isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, BQ643363.1, AK025485.1 and AL133215.17. On May 31, 2019 this sequence version replaced NM_001163813.1. Transcript Variant: This variant (3) uses an alternate splice site in exon 1 that results in the use of an in-frame downstream start codon, compared to variant 1. The encoded protein (isoform C) lacks most of the N-terminus, which contains a mitochondrial targeting sequence and probable ssDNA binding domain, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK297068.1, SRR1803611.169698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
    GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
    GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
    AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
    GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
    GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157285.1
    CDS191..883
    Translation

    Target ORF information:

    RefSeq Version NM_001163813.2
    Organism Homo sapiens(human)
    Definition Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001163813.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
    GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
    GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
    AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
    GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
    GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26432
    Clone ID Related Accession (Same CDS sequence) NM_001368275.1 , XM_011539975.2 , NM_001163813.2 , XM_017016437.1 , NM_001163813.1
    Accession Version NM_001163813.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product twinkle protein, mitochondrial isoform C
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, BQ643363.1 and AK025485.1. Transcript Variant: This variant (3) uses an alternate splice site in exon 1 that results in the use of an in-frame downstream start codon, compared to variant 1. The encoded protein (isoform C) lacks most of the N-terminus, which contains a mitochondrial targeting sequence and probable ssDNA binding domain, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163655.349144.1, SRR1803611.169698.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
    GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
    GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
    AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
    GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
    GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157285.1
    CDS198..890
    Translation

    Target ORF information:

    RefSeq Version NM_001163813.1
    Organism Homo sapiens(human)
    Definition Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001163813.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
    GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
    GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
    AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
    GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
    GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26432
    Clone ID Related Accession (Same CDS sequence) NM_001368275.1 , XM_011539975.2 , NM_001163813.2 , XM_017016437.1 , NM_001163813.1
    Accession Version XM_011539975.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product twinkle protein, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011539975.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
    GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
    GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
    AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
    GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
    GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011538277.1
    CDS145..837
    Translation

    Target ORF information:

    RefSeq Version XM_011539975.2
    Organism Homo sapiens(human)
    Definition Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011539975.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
    GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
    GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
    AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
    GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
    GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26432
    Clone ID Related Accession (Same CDS sequence) NM_001368275.1 , XM_011539975.2 , NM_001163813.2 , XM_017016437.1 , NM_001163813.1
    Accession Version XM_017016437.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 693bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-03-25
    Organism Homo sapiens(human)
    Product twinkle protein, mitochondrial isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000010.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
    GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
    GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
    AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
    GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
    GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016871926.1
    CDS1475..2167
    Translation

    Target ORF information:

    RefSeq Version XM_017016437.1
    Organism Homo sapiens(human)
    Definition Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017016437.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGCAAGCCA GGAAGCAGAC AATGTTCTGA TCCTGCAGGA CAGGAAGCTG
    GTAACCGGGC CAGGGAAACG GTATCTGCAG GTGTCCAAGA ACCGCTTTGA TGGAGATGTA
    GGTGTCTTCC CGCTTGAGTT CAACAAGAAC TCCCTCACCT TCTCCATTCC ACCAAAGAAC
    AAGGCCCGGC TCAAGAAGAT CAAGGATGAC ACTGGACCAG TGGCCAAAAA GCCCTCTTCT
    GGCAAAAAGG GGGCTACGAC ACAGAACTCT GAGATTTGCT CAGGCCAGGC CCCCACTCCC
    GACCAGCCAG ACACCTCCAA GCGTTCAAAG TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22794
    Clone ID Related Accession (Same CDS sequence) NM_001163814.2 , NM_001163814.1
    Accession Version NM_001163814.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product twinkle protein, mitochondrial isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, AL133215.17 and AK025485.1. On May 31, 2019 this sequence version replaced NM_001163814.1. Transcript Variant: This variant (4) has multiple splice site differences, compared to variant 1. The encoded protein (isoform D) lacks most of the N-terminus, which contains a mitochondrial targeting sequence and probable ssDNA binding domain, and has a shorter and distinct C-terminus, which lacks a C-terminal domain required for hexamer formation, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR5189667.192749.1, AL529767.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGTGAGTGG CCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157286.1
    CDS191..577
    Translation

    Target ORF information:

    RefSeq Version NM_001163814.2
    Organism Homo sapiens(human)
    Definition Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001163814.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGTGAGTGG CCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22794
    Clone ID Related Accession (Same CDS sequence) NM_001163814.2 , NM_001163814.1
    Accession Version NM_001163814.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 387bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-22
    Organism Homo sapiens(human)
    Product twinkle protein, mitochondrial isoform D
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, AL133215.17 and AK025485.1. Transcript Variant: This variant (4) has multiple splice site differences, compared to variant 1. The encoded protein (isoform D) lacks most of the N-terminus, which contains a mitochondrial targeting sequence and probable ssDNA binding domain, and has a shorter and distinct C-terminus, which lacks a C-terminal domain required for hexamer formation, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AL529767.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1966682, SAMEA1968968 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGTGAGTGG CCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157286.1
    CDS198..584
    Translation

    Target ORF information:

    RefSeq Version NM_001163814.1
    Organism Homo sapiens(human)
    Definition Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001163814.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    ATGCTGACAC AGTTTGCCGA GGGGCGGCTG GAAGATCAAC TGGACAAATA TGATCACTGG 
    GCTGACCGCT TTGAGGACCT GCCCCTCTAT TTCATGACTT TCCATGGACA GCAAAGCATC
    AGGACTGTAA TAGATACAAT GCAACATGCA GTCTACGTCT ATGACATTTG TCATGTGATC
    ATCGACAACC TGCAGTTCAT GATGGGTCAC GAGCAGCTGT CCACAGACAG GATCGCAGCT
    CAAGACTACA TCATCGGGGT CTTTCGGAAG TTTGCAACAG ACAATAACTG CCATGTGACA
    CTGGTCATTC ACCCCCGGAA AGAGGATGAT GACAAGGAAC TGCAGACAGC GTCCATTTTT
    GGCTCAGCCA AAGTGAGTGG CCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26964
    Clone ID Related Accession (Same CDS sequence) NM_001163812.2 , NM_001163812.1
    Accession Version NM_001163812.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1749bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-09
    Organism Homo sapiens(human)
    Product twinkle protein, mitochondrial isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB090322.1, AF292005.1, BC013349.2, AK025485.1 and AL133215.17. On May 31, 2019 this sequence version replaced NM_001163812.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region which introduces a novel stop codon, compared to variant 1. The encoded protein (isoform B; also known as Twinky) has a shorter and distinct C-terminus, which lacks a C-terminal domain required for hexamer formation, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1714090.1, SRR1803616.64312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC
    AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC
    CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA
    CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC
    AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT
    CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA
    GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT
    GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT
    GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG
    CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA
    CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG
    GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC
    CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG
    TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG
    TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC
    CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG
    CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA
    CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT
    ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA
    AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG
    TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC
    GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC
    CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA
    ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC
    ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG
    GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG
    AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGTGAGT
    GGCCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157284.1
    CDS669..2417
    Translation

    Target ORF information:

    RefSeq Version NM_001163812.2
    Organism Homo sapiens(human)
    Definition Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001163812.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC
    AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC
    CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA
    CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC
    AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT
    CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA
    GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT
    GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT
    GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG
    CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA
    CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG
    GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC
    CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG
    TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG
    TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC
    CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG
    CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA
    CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT
    ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA
    AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG
    TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC
    GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC
    CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA
    ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC
    ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG
    GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG
    AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGTGAGT
    GGCCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu26964
    Clone ID Related Accession (Same CDS sequence) NM_001163812.2 , NM_001163812.1
    Accession Version NM_001163812.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1749bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-21
    Organism Homo sapiens(human)
    Product twinkle protein, mitochondrial isoform B
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CB112401.1, DB090322.1, AF292005.1, BC013349.2 and AK025485.1. Transcript Variant: This variant (2) uses an alternate splice site in the 3' coding region which introduces a novel stop codon, compared to variant 1. The encoded protein (isoform B; also known as Twinky) has a shorter and distinct C-terminus, which lacks a C-terminal domain required for hexamer formation, compared to isoform A. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.12391.1, SRR1803616.64312.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## gene product(s) localized to mito. :: reported by MitoCarta ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC
    AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC
    CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA
    CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC
    AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT
    CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA
    GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT
    GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT
    GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG
    CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA
    CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG
    GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC
    CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG
    TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG
    TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC
    CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG
    CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA
    CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT
    ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA
    AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG
    TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC
    GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC
    CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA
    ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC
    ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG
    GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG
    AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGTGAGT
    GGCCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001157284.1
    CDS676..2424
    Translation

    Target ORF information:

    RefSeq Version NM_001163812.1
    Organism Homo sapiens(human)
    Definition Homo sapiens twinkle mtDNA helicase (TWNK), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001163812.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    ATGTGGGTCC TCCTCCGAAG TGGGTACCCC CTCCGTATCT TGTTACCCCT GCGTGGGGAG 
    TGGATGGGTC GGAGGGGCCT GCCCCGAAAC TTGGCCCCAG GCCCTCCTCG CAGACGTTAC
    AGGAAGGAGA CTCTCCAAGC CTTGGATATG CCAGTGTTGC CTGTAACTGC AACTGAAATC
    CGCCAGTATT TGCGGGGGCA TGGGATCCCC TTCCAGGATG GTCACAGTTG CCTGCGGGCA
    CTGAGCCCCT TTGCAGAGTC TTCACAGCTC AAAGGCCAGA CTGGTGTTAC CACTTCCTTC
    AGCCTCTTCA TTGACAAGAC CACAGGCCAC TTTCTCTGCA TGACCAGCCT AGCAGAAGGG
    AGCTGGGAAG ACTTCCAGGC CAGCGTGGAG GGGCGAGGGG ATGGGGCCAG GGAGGGGTTT
    CTGCTTAGCA AGGCACCAGA ATTTGAGGAC AGCGAGGAGG TCCGGAGGAT CTGGAACCGA
    GCAATACCTC TCTGGGAGCT GCCTGATCAG GAGGAGGTTC AGCTGGCTGA TACAATGTTT
    GGCCTTACCA AGGTTACAGA TGACACACTC AAGCGTTTCA GTGTGCGATA TCTGCGACCT
    GCTCGCAGTC TTGTCTTCCC TTGGTTCTCC CCTGGGGGCT CAGGATTACG AGGCCTGAAG
    CTCCTAGAGG CTAAATGCCA GGGGGATGGA GTGAGCTACG AGGAAACCAC TATTCCCCGA
    CCCAGCGCCT ACCACAATCT GTTTGGATTA CCACTGATTA GTCGTCGAGA TGCTGAGGTG
    GTACTGACGA GTCGTGAGCT TGACAGCCTG GCCTTGAACC AGTCCACGGG GCTGCCTACC
    CTTACTCTAC CCCGAGGAAC GACCTGCTTA CCCCCTGCCT TACTCCCTTA CCTGGAACAG
    TTCCGGCGGA TTGTATTCTG GTTGGGGGAT GACCTTCGGT CCTGGGAAGC CGCCAAGTTG
    TTTGCACGAA AACTGAACCC CAAACGATGC TTCTTGGTGC GACCAGGAGA CCAGCAACCC
    CGTCCCCTGG AGGCCCTGAA CGGAGGCTTC AATCTTTCTC GTATTCTTCG TACCGCCCTG
    CCTGCCTGGC ACAAGTCCAT CGTATCTTTC CGGCAGCTTC GGGAGGAGGT GCTAGGAGAA
    CTGTCAAATG TGGAGCAAGC AGCTGGCCTC CGCTGGAGCC GCTTTCCAGA CCTCAATCGT
    ATCTTGAAGG GACATCGAAA GGGCGAGCTG ACGGTCTTCA CAGGGCCAAC AGGCAGTGGA
    AAGACGACAT TCATCAGTGA GTATGCCCTG GATTTGTGTT CCCAGGGGGT GAACACACTG
    TGGGGTAGCT TCGAGATCAG CAATGTGAGA CTAGCCCGGG TCATGCTGAC ACAGTTTGCC
    GAGGGGCGGC TGGAAGATCA ACTGGACAAA TATGATCACT GGGCTGACCG CTTTGAGGAC
    CTGCCCCTCT ATTTCATGAC TTTCCATGGA CAGCAAAGCA TCAGGACTGT AATAGATACA
    ATGCAACATG CAGTCTACGT CTATGACATT TGTCATGTGA TCATCGACAA CCTGCAGTTC
    ATGATGGGTC ACGAGCAGCT GTCCACAGAC AGGATCGCAG CTCAAGACTA CATCATCGGG
    GTCTTTCGGA AGTTTGCAAC AGACAATAAC TGCCATGTGA CACTGGTCAT TCACCCCCGG
    AAAGAGGATG ATGACAAGGA ACTGCAGACA GCGTCCATTT TTGGCTCAGC CAAAGTGAGT
    GGCCTTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.