PRKX cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PRKX
Entrez Gene ID	5613
Full Name	protein kinase, X-linked
Synonyms	PKX1
General protein information	Preferred Names protein kinase, X-linked Names cAMP-dependent protein kinase catalytic subunit PRKX protein kinase PKX1 protein kinase X serine/threonine-protein kinase PRKX
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xp22.33
Summary	This gene encodes a serine threonine protein kinase that has similarity to the catalytic subunit of cyclic AMP dependent protein kinases. The encoded protein is developmentally regulated and may be involved in renal epithelial morphogenesis. This protein may also be involved in macrophage and granulocyte maturation. Abnormal recombination between this gene and a related pseudogene on chromosome Y is a frequent cause of sex reversal disorder in XX males and XY females. Pseudogenes of this gene are found on chromosomes X, 15 and Y. [provided by RefSeq, Feb 2010].
Disorder MIM:	300083

mRNA and Protein(s)

mRNA	Protein	Name
NM_005044.5	NP_005035.1	cAMP-dependent protein kinase catalytic subunit PRKX
NM_005044.4	NP_005035.1	cAMP-dependent protein kinase catalytic subunit PRKX

Pathways from BioSystems

Homologies

Homo sapiens (human)	PRKX	NP_005035.1
Caenorhabditis elegans (roundworm)	F47F2.1	NP_508671.2
	PRKX	XP_002806173.1
Bos taurus (cattle)	PRKX	XP_002700469.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011840	XP_320677.3
Pan troglodytes (chimpanzee)	PRKX	NP_001180591.1
Canis lupus familiaris (dog)	PRKX	XP_005642123.1
Drosophila melanogaster (fruit fly)	Pka-C3	NP_730083.2
Mus musculus (house mouse)	Prkx	NP_058675.1
Rattus norvegicus (Norway rat)	Prkx	NP_001029135.1
Danio rerio (zebrafish)	LOC561941	XP_690430.2
Gallus gallus (chicken)	PRKX	XP_416852.4
Xenopus tropicalis (tropical clawed frog)	prkx	XP_002941045.1

Gene Ontology
Bibliography

Related articles in PubMed

Altered Expression of PRKX, WNT3 and WNT16 in Human Nodular Basal Cell Carcinoma.
DO Carmo NG, Sakamoto LH, Pogue R, DO Couto Mascarenhas C, Passos SK, Felipe MS, DE Andrade RV
Anticancer research36(9)4545-51(2016 09)

PRKX, a Novel cAMP-Dependent Protein Kinase Member, Plays an Important Role in Development.
Huang S, Li Q, Alberts I, Li X
Journal of cellular biochemistry117(3)566-73(2016 Mar)

Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition.
Taipale M, Krykbaeva I, Koeva M, Kayatekin C, Westover KD, Karras GI, Lindquist S
Cell150(5)987-1001(2012 Aug)

PRKX, TTBK2 and RSK4 expression causes Sunitinib resistance in kidney carcinoma- and melanoma-cell lines.
Bender C, Ullrich A
International journal of cancer131(2)E45-55(2012 Jul)

MBD 4--a potential substrate for protein kinase X.
Lin Y, Li W
Acta biochimica et biophysica Sinica43(11)916-7(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

PRKX, WNT3 and WNT16 genes, belonging to the WNT signaling pathway, are involved in the tumorigenic process of nodular basal cell carcinoma
Title: Altered Expression of PRKX, WNT3 and WNT16 in Human Nodular Basal Cell Carcinoma.

The characteristics and developmental functions of PRKX. [review]
Title: PRKX, a Novel cAMP-Dependent Protein Kinase Member, Plays an Important Role in Development.

findings reveals a major role of PRKX, TTBK2 and RSK4 in triggering Sunitinib resistance formation; data suggest transcriptional regulation of these kinases together with other proteins might play an important role in formation of Sunitinib resistance by affecting transcription factors
Title: PRKX, TTBK2 and RSK4 expression causes Sunitinib resistance in kidney carcinoma- and melanoma-cell lines.

MBD 4--a potential substrate for protein kinase X
Title: MBD 4--a potential substrate for protein kinase X.

The interaction of PRKX with Pin-1, Magi-1 and Bag-3 could contribute to the stimulating role of PRKX in angiogenesis.
Title: PRKX critically regulates endothelial cell proliferation, migration, and vascular-like structure formation.

The following PRKX gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PRKX cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu21260	NM_005044.5 Latest version!	Homo sapiens protein kinase X-linked (PRKX), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
View Old Accession Versions >>
OHu21260	NM_005044.4	Homo sapiens protein kinase X-linked (PRKX), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PRKX ( NM_005044.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_005035.1 Homo sapiens protein kinase X-linked (PRKX), mRNA.

Price & Availability↑

CloneID	OHu21260
Clone ID Related Accession (Same CDS sequence)	NM_005044.5 , NM_005044.4
Accession Version	NM_005044.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-01
Organism	Homo sapiens(human)
Product	cAMP-dependent protein kinase catalytic subunit PRKX
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007685.2, DN997580.1, BE675757.1 and BC041073.1. This sequence is a reference standard in the RefSeqGene project. On May 17, 2019 this sequence version replaced NM_005044.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X85545.1, SRR1660807.126259.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000262848.6/ ENSP00000262848.5 RefSeq Select criteria :: based on single protein-coding transcript ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGC CCGGGCTGGC CCAGGCGGCC GCGGCGGAGA GCGACTCCCG CAAGGTGGCG 
GAGGAGACCC CCGACGGGGC GCCCGCGCTC TGCCCCAGCC CTGAGGCGCT GTCGCCGGAG 
CCGCCTGTGT ACAGCCTGCA GGACTTTGAC ACGCTGGCCA CCGTGGGCAC TGGGACGTTC 
GGGCGGGTGC ACCTGGTGAA GGAGAAGACA GCCAAGCATT TCTTCGCCCT CAAGGTGATG 
AGCATTCCCG ACGTCATCCG CCTAAAGCAG GAGCAACACG TACACAATGA GAAGTCTGTC 
CTGAAGGAAG TCAGCCACCC GTTCCTCATC AGGCTGTTCT GGACGTGGCA TGACGAGCGC 
TTCCTCTACA TGCTCATGGA GTACGTGCCG GGCGGCGAGC TCTTCAGCTA CCTGCGCAAC 
CGGGGGCGCT TCTCCAGCAC CACGGGGCTC TTCTACTCTG CAGAGATCAT CTGTGCCATC 
GAGTACCTGC ACTCCAAAGA GATCGTCTAC AGGGACTTGA AGCCAGAGAA CATCCTGCTG 
GATAGGGATG GCCACATTAA GCTCACGGAC TTTGGGTTCG CCAAGAAGCT GGTAGACAGG 
ACTTGGACCC TCTGTGGAAC ACCCGAGTAC CTAGCCCCCG AAGTCATTCA GAGCAAGGGC 
CACGGAAGGG CCGTGGACTG GTGGGCCCTC GGCATCCTGA TATTCGAGAT GCTTTCGGGG 
TTTCCTCCGT TTTTTGATGA CAACCCGTTT GGCATTTATC AGAAAATTCT TGCAGGCAAA 
ATAGATTTCC CCAGACATTT GGATTTCCAT GTAAAAGACC TCATTAAGAA ACTGCTCGTG 
GTTGACAGAA CAAGGCGATT AGGAAACATG AAGAACGGGG CGAATGATGT GAAGCATCAT 
CGGTGGTTCC GCTCCGTGGA CTGGGAAGCT GTTCCGCAGA GAAAACTGAA GCCTCCCATC 
GTGCCCAAGA TAGCTGGTGA CGGCGACACT TCCAACTTCG AAACTTACCC TGAGAATGAC 
TGGGACACAG CCGCGCCCGT GCCGCAGAAG GATTTAGAAA TCTTCAAGAA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005035.1
CDS	397..1473
Translation	MEAPGLAQAAAAESDSRKVAEETPDGAPALCPSPEALSPEPPVYSLQDFDTLATVGTGTFGRVHLVKEKTAKHFFALKVMSIPDVIRLKQEQHVHNEKSVLKEVSHPFLIRLFWTWHDERFLYMLMEYVPGGELFSYLRNRGRFSSTTGLFYSAEIICAIEYLHSKEIVYRDLKPENILLDRDGHIKLTDFGFAKKLVDRTWTLCGTPEYLAPEVIQSKGHGRAVDWWALGILIFEMLSGFPPFFDDNPFGIYQKILAGKIDFPRHLDFHVKDLIKKLLVVDRTRRLGNMKNGANDVKHHRWFRSVDWEAVPQRKLKPPIVPKIAGDGDTSNFETYPENDWDTAAPVPQKDLEIFKNF

Target ORF information:

RefSeq Version	NM_005044.5
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase X-linked (PRKX), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005044.5

ORF Insert Sequence:

ATGGAGGCGC CCGGGCTGGC CCAGGCGGCC GCGGCGGAGA GCGACTCCCG CAAGGTGGCG 
GAGGAGACCC CCGACGGGGC GCCCGCGCTC TGCCCCAGCC CTGAGGCGCT GTCGCCGGAG 
CCGCCTGTGT ACAGCCTGCA GGACTTTGAC ACGCTGGCCA CCGTGGGCAC TGGGACGTTC 
GGGCGGGTGC ACCTGGTGAA GGAGAAGACA GCCAAGCATT TCTTCGCCCT CAAGGTGATG 
AGCATTCCCG ACGTCATCCG CCTAAAGCAG GAGCAACACG TACACAATGA GAAGTCTGTC 
CTGAAGGAAG TCAGCCACCC GTTCCTCATC AGGCTGTTCT GGACGTGGCA TGACGAGCGC 
TTCCTCTACA TGCTCATGGA GTACGTGCCG GGCGGCGAGC TCTTCAGCTA CCTGCGCAAC 
CGGGGGCGCT TCTCCAGCAC CACGGGGCTC TTCTACTCTG CAGAGATCAT CTGTGCCATC 
GAGTACCTGC ACTCCAAAGA GATCGTCTAC AGGGACTTGA AGCCAGAGAA CATCCTGCTG 
GATAGGGATG GCCACATTAA GCTCACGGAC TTTGGGTTCG CCAAGAAGCT GGTAGACAGG 
ACTTGGACCC TCTGTGGAAC ACCCGAGTAC CTAGCCCCCG AAGTCATTCA GAGCAAGGGC 
CACGGAAGGG CCGTGGACTG GTGGGCCCTC GGCATCCTGA TATTCGAGAT GCTTTCGGGG 
TTTCCTCCGT TTTTTGATGA CAACCCGTTT GGCATTTATC AGAAAATTCT TGCAGGCAAA 
ATAGATTTCC CCAGACATTT GGATTTCCAT GTAAAAGACC TCATTAAGAA ACTGCTCGTG 
GTTGACAGAA CAAGGCGATT AGGAAACATG AAGAACGGGG CGAATGATGT GAAGCATCAT 
CGGTGGTTCC GCTCCGTGGA CTGGGAAGCT GTTCCGCAGA GAAAACTGAA GCCTCCCATC 
GTGCCCAAGA TAGCTGGTGA CGGCGACACT TCCAACTTCG AAACTTACCC TGAGAATGAC 
TGGGACACAG CCGCGCCCGT GCCGCAGAAG GATTTAGAAA TCTTCAAGAA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PRKX ( NM_005044.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_005035.1 Homo sapiens protein kinase X-linked (PRKX), mRNA.

Price & Availability↑

CloneID	OHu21260
Clone ID Related Accession (Same CDS sequence)	NM_005044.5 , NM_005044.4
Accession Version	NM_005044.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1077bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Homo sapiens(human)
Product	cAMP-dependent protein kinase catalytic subunit PRKX
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DN997580.1, BE675757.1, BC041073.1 and AC007685.2. This sequence is a reference standard in the RefSeqGene project. On Mar 5, 2010 this sequence version replaced NM_005044.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: X85545.1, SRR1660807.126259.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAGGCGC CCGGGCTGGC CCAGGCGGCC GCGGCGGAGA GCGACTCCCG CAAGGTGGCG 
GAGGAGACCC CCGACGGGGC GCCCGCGCTC TGCCCCAGCC CTGAGGCGCT GTCGCCGGAG 
CCGCCTGTGT ACAGCCTGCA GGACTTTGAC ACGCTGGCCA CCGTGGGCAC TGGGACGTTC 
GGGCGGGTGC ACCTGGTGAA GGAGAAGACA GCCAAGCATT TCTTCGCCCT CAAGGTGATG 
AGCATTCCCG ACGTCATCCG CCTAAAGCAG GAGCAACACG TACACAATGA GAAGTCTGTC 
CTGAAGGAAG TCAGCCACCC GTTCCTCATC AGGCTGTTCT GGACGTGGCA TGACGAGCGC 
TTCCTCTACA TGCTCATGGA GTACGTGCCG GGCGGCGAGC TCTTCAGCTA CCTGCGCAAC 
CGGGGGCGCT TCTCCAGCAC CACGGGGCTC TTCTACTCTG CAGAGATCAT CTGTGCCATC 
GAGTACCTGC ACTCCAAAGA GATCGTCTAC AGGGACTTGA AGCCAGAGAA CATCCTGCTG 
GATAGGGATG GCCACATTAA GCTCACGGAC TTTGGGTTCG CCAAGAAGCT GGTAGACAGG 
ACTTGGACCC TCTGTGGAAC ACCCGAGTAC CTAGCCCCCG AAGTCATTCA GAGCAAGGGC 
CACGGAAGGG CCGTGGACTG GTGGGCCCTC GGCATCCTGA TATTCGAGAT GCTTTCGGGG 
TTTCCTCCGT TTTTTGATGA CAACCCGTTT GGCATTTATC AGAAAATTCT TGCAGGCAAA 
ATAGATTTCC CCAGACATTT GGATTTCCAT GTAAAAGACC TCATTAAGAA ACTGCTCGTG 
GTTGACAGAA CAAGGCGATT AGGAAACATG AAGAACGGGG CGAATGATGT GAAGCATCAT 
CGGTGGTTCC GCTCCGTGGA CTGGGAAGCT GTTCCGCAGA GAAAACTGAA GCCTCCCATC 
GTGCCCAAGA TAGCTGGTGA CGGCGACACT TCCAACTTCG AAACTTACCC TGAGAATGAC 
TGGGACACAG CCGCGCCCGT GCCGCAGAAG GATTTAGAAA TCTTCAAGAA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_005035.1
CDS	382..1458
Translation	MEAPGLAQAAAAESDSRKVAEETPDGAPALCPSPEALSPEPPVYSLQDFDTLATVGTGTFGRVHLVKEKTAKHFFALKVMSIPDVIRLKQEQHVHNEKSVLKEVSHPFLIRLFWTWHDERFLYMLMEYVPGGELFSYLRNRGRFSSTTGLFYSAEIICAIEYLHSKEIVYRDLKPENILLDRDGHIKLTDFGFAKKLVDRTWTLCGTPEYLAPEVIQSKGHGRAVDWWALGILIFEMLSGFPPFFDDNPFGIYQKILAGKIDFPRHLDFHVKDLIKKLLVVDRTRRLGNMKNGANDVKHHRWFRSVDWEAVPQRKLKPPIVPKIAGDGDTSNFETYPENDWDTAAPVPQKDLEIFKNF

Target ORF information:

RefSeq Version	NM_005044.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase X-linked (PRKX), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_005044.4

ORF Insert Sequence:

ATGGAGGCGC CCGGGCTGGC CCAGGCGGCC GCGGCGGAGA GCGACTCCCG CAAGGTGGCG 
GAGGAGACCC CCGACGGGGC GCCCGCGCTC TGCCCCAGCC CTGAGGCGCT GTCGCCGGAG 
CCGCCTGTGT ACAGCCTGCA GGACTTTGAC ACGCTGGCCA CCGTGGGCAC TGGGACGTTC 
GGGCGGGTGC ACCTGGTGAA GGAGAAGACA GCCAAGCATT TCTTCGCCCT CAAGGTGATG 
AGCATTCCCG ACGTCATCCG CCTAAAGCAG GAGCAACACG TACACAATGA GAAGTCTGTC 
CTGAAGGAAG TCAGCCACCC GTTCCTCATC AGGCTGTTCT GGACGTGGCA TGACGAGCGC 
TTCCTCTACA TGCTCATGGA GTACGTGCCG GGCGGCGAGC TCTTCAGCTA CCTGCGCAAC 
CGGGGGCGCT TCTCCAGCAC CACGGGGCTC TTCTACTCTG CAGAGATCAT CTGTGCCATC 
GAGTACCTGC ACTCCAAAGA GATCGTCTAC AGGGACTTGA AGCCAGAGAA CATCCTGCTG 
GATAGGGATG GCCACATTAA GCTCACGGAC TTTGGGTTCG CCAAGAAGCT GGTAGACAGG 
ACTTGGACCC TCTGTGGAAC ACCCGAGTAC CTAGCCCCCG AAGTCATTCA GAGCAAGGGC 
CACGGAAGGG CCGTGGACTG GTGGGCCCTC GGCATCCTGA TATTCGAGAT GCTTTCGGGG 
TTTCCTCCGT TTTTTGATGA CAACCCGTTT GGCATTTATC AGAAAATTCT TGCAGGCAAA 
ATAGATTTCC CCAGACATTT GGATTTCCAT GTAAAAGACC TCATTAAGAA ACTGCTCGTG 
GTTGACAGAA CAAGGCGATT AGGAAACATG AAGAACGGGG CGAATGATGT GAAGCATCAT 
CGGTGGTTCC GCTCCGTGGA CTGGGAAGCT GTTCCGCAGA GAAAACTGAA GCCTCCCATC 
GTGCCCAAGA TAGCTGGTGA CGGCGACACT TCCAACTTCG AAACTTACCC TGAGAATGAC 
TGGGACACAG CCGCGCCCGT GCCGCAGAAG GATTTAGAAA TCTTCAAGAA TTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Altered Expression of PRKX, WNT3 and WNT16 in Human Nodular Basal Cell Carcinoma.
Anticancer research36(9)4545-51(2016 09)
DO Carmo NG,Sakamoto LH,Pogue R,DO Couto Mascarenhas C,Passos SK,Felipe MS,DE Andrade RV

PRKX, a Novel cAMP-Dependent Protein Kinase Member, Plays an Important Role in Development.
Journal of cellular biochemistry117(3)566-73(2016 Mar)
Huang S,Li Q,Alberts I,Li X

Quantitative analysis of HSP90-client interactions reveals principles of substrate recognition.
Cell150(5)987-1001(2012 Aug)
Taipale M,Krykbaeva I,Koeva M,Kayatekin C,Westover KD,Karras GI,Lindquist S

PRKX, TTBK2 and RSK4 expression causes Sunitinib resistance in kidney carcinoma- and melanoma-cell lines.
International journal of cancer131(2)E45-55(2012 Jul)
Bender C,Ullrich A

MBD 4--a potential substrate for protein kinase X.
Acta biochimica et biophysica Sinica43(11)916-7(2011 Nov)
Lin Y,Li W

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PRKX cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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