NKRF cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	NKRF
Entrez Gene ID	55922
Full Name	NFKB repressing factor
Synonyms	ITBA4,NRF
General protein information	Preferred Names NFKB repressing factor Names NF-kappa-B-repressing factor transcription factor NRF
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: X Map Location: Xq24
Summary	This gene encodes a transcriptional repressor that interacts with specific negative regulatory elements to mediate transcriptional repression of certain nuclear factor kappa B responsive genes. The protein localizes predominantly to the nucleolus with a small fraction found in the nucleoplasm and cytoplasm. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Mar 2010].
Disorder MIM:	300440

mRNA and Protein(s)

mRNA	Protein	Name
XM_011531365.1	XP_011529667.1	NF-kappa-B-repressing factor isoform X1
NM_017544.5	NP_060014.3	NF-kappa-B-repressing factor
NM_017544.3	NP_060014.2	NF-kappa-B-repressing factor isoform 2
NM_017544.4	NP_060014.2	NF-kappa-B-repressing factor isoform 2
NM_001173487.1	NP_001166958.1	NF-kappa-B-repressing factor isoform 1
NM_001173488.1	NP_001166959.1	NF-kappa-B-repressing factor isoform 2
XM_011531365.2	XP_011529667.1	NF-kappa-B-repressing factor isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	NKRF	XP_001135674.2
Bos taurus (cattle)	NKRF	NP_001096023.1
Mus musculus (house mouse)	Nkrf	NP_084167.2
Gallus gallus (chicken)	NKRF	NP_001012905.1
Xenopus tropicalis (tropical clawed frog)	nkrf	NP_001039125.1
Homo sapiens (human)	NKRF	NP_001166958.1
	NKRF	XP_001082426.1
Canis lupus familiaris (dog)	NKRF	XP_003435703.2
Danio rerio (zebrafish)	nkrf	NP_001004499.2

Gene Ontology
Bibliography

Related articles in PubMed

Characterization of host proteins interacting with the lymphocytic choriomeningitis virus L protein.
Khamina K, Lercher A, Caldera M, Schliehe C, Vilagos B, Sahin M, Kosack L, Bhattacharya A, M?jek P, Stukalov A, Sacco R, James LC, Pinschewer DD, Bennett KL, Menche J, Bergthaler A
PLoS pathogens13(12)e1006758(2017 Dec)

Protein interaction network of alternatively spliced NudCD1 isoforms.
Asselin-Mullen P, Chauvin A, Dubois ML, Drissi R, L?vesque D, Boisvert FM
Scientific reports7(1)12987(2017 Oct)

The G-patch protein NF-?B-repressing factor mediates the recruitment of the exonuclease XRN2 and activation of the RNA helicase DHX15 in human ribosome biogenesis.
Memet I, Doebele C, Sloan KE, Bohnsack MT
Nucleic acids research45(9)5359-5374(2017 May)

Human NF-?B repressing factor acts as a stress-regulated switch for ribosomal RNA processing and nucleolar homeostasis surveillance.
Coccia M, Rossi A, Riccio A, Trotta E, Santoro MG
Proceedings of the National Academy of Sciences of the United States of America114(5)1045-1050(2017 Jan)

NF-?B-repressing factor phosphorylation regulates transcription elongation via its interactions with 5'?3' exoribonuclease 2 and negative elongation factor.
Rother S, Bartels M, Schweda AT, Resch K, Pallua N, Nourbakhsh M
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(1)174-85(2016 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data suggest that L protein from LCMV interactions with host proteome, specifically DDX3X, NKRF, and TRIM21. (LCMV = Lymphocytic choriomeningitis mammarenavirus; DDX3X = DEAD-box helicase 3; NKRF = NF-kappa-B-repressing factor; TRIM21 = tripartite motif-containing protein-21)
Title: Characterization of host proteins interacting with the lymphocytic choriomeningitis virus L protein.

depletion of NKRF, XRN2 or DHX15 impairs an early pre-rRNA cleavage step.
Title: The G-patch protein NF-?B-repressing factor mediates the recruitment of the exonuclease XRN2 and activation of the RNA helicase DHX15 in human ribosome biogenesis.

NF-kappaB-repressing factor (NKRF) phosphorylation modulates promoter-proximal transcription elongation of NF-kappaB/NKRF-regulated genes via direct interactions with elongation complex in response to specific stimuli
Title: NF-?B-repressing factor phosphorylation regulates transcription elongation via its interactions with 5'?3' exoribonuclease 2 and negative elongation factor.

MIR29 targets and reduces expression of CLDN1 and NKRF to increase intestinal permeability in inflammatory bowel disease.
Title: MicroRNA 29 targets nuclear factor-?B-repressing factor and Claudin 1 to increase intestinal permeability.

The up-regulated NKRF serves as an endogenous repressor for IP-10 and IL-8 synthesis to hinder host from robust response to MTb infection.
Title: NF-?B repressing factor downregulates basal expression and mycobacterium tuberculosis induced IP-10 and IL-8 synthesis via interference with NF-?B in monocytes.

The following NKRF gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the NKRF cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu11903	NM_017544.4 Latest version!	Homo sapiens NFKB repressing factor (NKRF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu11903	NM_001173488.1 Latest version!	Homo sapiens NFKB repressing factor (NKRF), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu11903	XM_011531365.2 Latest version!	Homo sapiens NFKB repressing factor (NKRF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu04603	NM_001173487.1 Latest version!	Homo sapiens NFKB repressing factor (NKRF), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$496.30 $709.00
OHu109248	NM_017544.5 Latest version!	Homo sapiens NFKB repressing factor (NKRF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu11903	NM_017544.3	Homo sapiens NFKB repressing factor (NKRF), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu11903	XM_011531365.1	Homo sapiens NFKB repressing factor (NKRF), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

NKRF ( NM_017544.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_060014.2 Homo sapiens NFKB repressing factor (NKRF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu11903
Clone ID Related Accession (Same CDS sequence)	XM_011531365.1 , NM_017544.3 , NM_017544.4 , NM_001173488.1 , XM_011531365.2
Accession Version	NM_017544.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Homo sapiens(human)
Product	NF-kappa-B-repressing factor isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY208891.1 and AA845622.1. This sequence is a reference standard in the RefSeqGene project. On Mar 16, 2010 this sequence version replaced NM_017544.2. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY208891.1, SRR1803611.179890.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAAAA TTCTCCAAAT GGCTGAAGGT ATTGATATTG GGGAGATGCC TTCATATGAT 
CTGGTGCTGT CCAAACCTTC CAAAGGTCAA AAACGCCACC TCTCAACATG TGATGGTCAA 
AATCCTCCTA AAAAGCAAGC CGGTTCCAAA TTCCATGCGA GACCTCGTTT TGAGCCTGTA 
CATTTTGTAG CTAGTAGTTC AAAAGATGAA AGACAGGAAG ATCCTTATGG CCCTCAAACA 
AAAGAGGTAA ATGAACAAAC ACATTTTGCC AGCATGCCAA GAGACATCTA CCAAGATTAT 
ACTCAAGACT CTTTCAGTAT ACAAGATGGG AATTCTCAGT ATTGTGATTC ATCAGGATTC 
ATTCTCACAA AAGACCAGCC TGTAACAGCC AACATGTATT TTGACAGTGG GAACCCTGCC 
CCAAGCACCA CATCACAGCA GGCAAACTCT CAGTCAACTC CTGAGCCTTC ACCATCACAG 
ACATTTCCCG AGTCTGTGGT AGCCGAGAAG CAGTATTTTA TTGAAAAATT AACGGCGACA 
ATCTGGAAGA ACCTTTCTAA TCCAGAAATG ACTTCTGGAT CTGATAAAAT TAATTATACA 
TATATGTTAA CTCGTTGTAT TCAGGCGTGT AAGACAAATC CTGAGTATAT ATATGCTCCT 
TTAAAGGAAA TTCCTCCTGC CGACATCCCC AAAAATAAAA AACTTCTAAC TGATGGCTAT 
GCTTGTGAAG TTAGATGCCA AAATATCTAC TTAACTACAG GTTATGCTGG CAGCAAGAAT 
GGGTCCAGGG ATCGAGCTAC AGAGCTAGCT GTAAAACTCT TGCAGAAACG TATTGAAGTT 
AGAGTTGTCC GGCGGAAATT CAAGCATACA TTTGGAGAGG ACCTCGTGGT GTGTCAGATT 
GGCATGTCCT CCTATGAATT TCCTCCAGCT CTGAAGCCAC CAGAAGACCT GGTGGTGCTG 
GGTAAAGATG CTTCCGGGCA GCCAATTTTT AATGCTTCTG CCAAACACTG GACCAATTTT 
GTCATTACAG AAAATGCAAA TGATGCAATT GGTATCCTTA ACAATTCTGC CTCATTCAAC 
AAGATGTCAA TTGAATACAA ATATGAGATG ATGCCAAATC GCACATGGCG TTGTCGAGTG 
TTTTTACAAG ATCACTGCTT AGCTGAAGGT TATGGAACCA AGAAAACAAG TAAACATGCA 
GCTGCCGACG AGGCTTTGAA AATTCTTCAA AAAACACAGC CCACTTATCC ATCTGTCAAA 
AGTTCACAAT GCCATACAGG CTCTTCACCC AGAGGATCTG GAAAGAAGAA AGATATAAAG 
GATCTTGTAG TTTATGAGAA TTCTTCAAAT CCCGTGTGCA CGCTGAACGA CACAGCTCAG 
TTTAACCGAA TGACAGTTGA GTATGTCTAT GAAAGGATGA CAGGCCTCCG CTGGAAATGC 
AAAGTGATTC TAGAGAGTGA AGTAATTGCA GAAGCAGTTG GGGTGAAGAA AACTGTCAAA 
TATGAAGCTG CTGGGGAAGC TGTGAAAACC CTCAAAAAGA CCCAGCCAAC TGTCATTAAC 
AACTTGAAGA AAGGAGCTGT TGAAGATGTG ATTTCAAGAA ATGAAATTCA GGGCCGCTCA 
GCAGAGGAGG CTTACAAACA GCAAATCAAA GAAGATAATA TTGGAAATCA GCTGCTGAGA 
AAGATGGGTT GGACTGGTGG TGGTTTAGGT AAATCTGGTG AGGGCATACG GGAGCCTATC 
TCAGTGAAAG AGCAGCATAA GCGGGAAGGG CTTGGTCTGG ATGTAGAGAG GGTGAATAAA 
ATTGCCAAGA GAGATATTGA ACAGATCATC AGAAACTACG CCCGCTCCGA GAGCCACACA 
GATTTGACTT TCTCTAGAGA GCTGACTAAT GATGAACGGA AGCAAATACA TCAGATTGCC 
CAGAAGTATG GTCTTAAGAG TAAGTCTCAT GGGGTGGGCC ATGATAGGTA CCTAGTGGTA 
GGTAGAAAAA GACGGAAGGA AGACCTACTA GATCAGCTCA AACAGGAAGG CCAAGTGGGC 
CATTACGAGC TTGTTATGCC TCAAGCAAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060014.2
CDS	170..2242
Translation	MEKILQMAEGIDIGEMPSYDLVLSKPSKGQKRHLSTCDGQNPPKKQAGSKFHARPRFEPVHFVASSSKDERQEDPYGPQTKEVNEQTHFASMPRDIYQDYTQDSFSIQDGNSQYCDSSGFILTKDQPVTANMYFDSGNPAPSTTSQQANSQSTPEPSPSQTFPESVVAEKQYFIEKLTATIWKNLSNPEMTSGSDKINYTYMLTRCIQACKTNPEYIYAPLKEIPPADIPKNKKLLTDGYACEVRCQNIYLTTGYAGSKNGSRDRATELAVKLLQKRIEVRVVRRKFKHTFGEDLVVCQIGMSSYEFPPALKPPEDLVVLGKDASGQPIFNASAKHWTNFVITENANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQAN

Target ORF information:

RefSeq Version	NM_017544.3
Organism	Homo sapiens(human)
Definition	Homo sapiens NFKB repressing factor (NKRF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017544.3

ORF Insert Sequence:

ATGGAAAAAA TTCTCCAAAT GGCTGAAGGT ATTGATATTG GGGAGATGCC TTCATATGAT 
CTGGTGCTGT CCAAACCTTC CAAAGGTCAA AAACGCCACC TCTCAACATG TGATGGTCAA 
AATCCTCCTA AAAAGCAAGC CGGTTCCAAA TTCCATGCGA GACCTCGTTT TGAGCCTGTA 
CATTTTGTAG CTAGTAGTTC AAAAGATGAA AGACAGGAAG ATCCTTATGG CCCTCAAACA 
AAAGAGGTAA ATGAACAAAC ACATTTTGCC AGCATGCCAA GAGACATCTA CCAAGATTAT 
ACTCAAGACT CTTTCAGTAT ACAAGATGGG AATTCTCAGT ATTGTGATTC ATCAGGATTC 
ATTCTCACAA AAGACCAGCC TGTAACAGCC AACATGTATT TTGACAGTGG GAACCCTGCC 
CCAAGCACCA CATCACAGCA GGCAAACTCT CAGTCAACTC CTGAGCCTTC ACCATCACAG 
ACATTTCCCG AGTCTGTGGT AGCCGAGAAG CAGTATTTTA TTGAAAAATT AACGGCGACA 
ATCTGGAAGA ACCTTTCTAA TCCAGAAATG ACTTCTGGAT CTGATAAAAT TAATTATACA 
TATATGTTAA CTCGTTGTAT TCAGGCGTGT AAGACAAATC CTGAGTATAT ATATGCTCCT 
TTAAAGGAAA TTCCTCCTGC CGACATCCCC AAAAATAAAA AACTTCTAAC TGATGGCTAT 
GCTTGTGAAG TTAGATGCCA AAATATCTAC TTAACTACAG GTTATGCTGG CAGCAAGAAT 
GGGTCCAGGG ATCGAGCTAC AGAGCTAGCT GTAAAACTCT TGCAGAAACG TATTGAAGTT 
AGAGTTGTCC GGCGGAAATT CAAGCATACA TTTGGAGAGG ACCTCGTGGT GTGTCAGATT 
GGCATGTCCT CCTATGAATT TCCTCCAGCT CTGAAGCCAC CAGAAGACCT GGTGGTGCTG 
GGTAAAGATG CTTCCGGGCA GCCAATTTTT AATGCTTCTG CCAAACACTG GACCAATTTT 
GTCATTACAG AAAATGCAAA TGATGCAATT GGTATCCTTA ACAATTCTGC CTCATTCAAC 
AAGATGTCAA TTGAATACAA ATATGAGATG ATGCCAAATC GCACATGGCG TTGTCGAGTG 
TTTTTACAAG ATCACTGCTT AGCTGAAGGT TATGGAACCA AGAAAACAAG TAAACATGCA 
GCTGCCGACG AGGCTTTGAA AATTCTTCAA AAAACACAGC CCACTTATCC ATCTGTCAAA 
AGTTCACAAT GCCATACAGG CTCTTCACCC AGAGGATCTG GAAAGAAGAA AGATATAAAG 
GATCTTGTAG TTTATGAGAA TTCTTCAAAT CCCGTGTGCA CGCTGAACGA CACAGCTCAG 
TTTAACCGAA TGACAGTTGA GTATGTCTAT GAAAGGATGA CAGGCCTCCG CTGGAAATGC 
AAAGTGATTC TAGAGAGTGA AGTAATTGCA GAAGCAGTTG GGGTGAAGAA AACTGTCAAA 
TATGAAGCTG CTGGGGAAGC TGTGAAAACC CTCAAAAAGA CCCAGCCAAC TGTCATTAAC 
AACTTGAAGA AAGGAGCTGT TGAAGATGTG ATTTCAAGAA ATGAAATTCA GGGCCGCTCA 
GCAGAGGAGG CTTACAAACA GCAAATCAAA GAAGATAATA TTGGAAATCA GCTGCTGAGA 
AAGATGGGTT GGACTGGTGG TGGTTTAGGT AAATCTGGTG AGGGCATACG GGAGCCTATC 
TCAGTGAAAG AGCAGCATAA GCGGGAAGGG CTTGGTCTGG ATGTAGAGAG GGTGAATAAA 
ATTGCCAAGA GAGATATTGA ACAGATCATC AGAAACTACG CCCGCTCCGA GAGCCACACA 
GATTTGACTT TCTCTAGAGA GCTGACTAAT GATGAACGGA AGCAAATACA TCAGATTGCC 
CAGAAGTATG GTCTTAAGAG TAAGTCTCAT GGGGTGGGCC ATGATAGGTA CCTAGTGGTA 
GGTAGAAAAA GACGGAAGGA AGACCTACTA GATCAGCTCA AACAGGAAGG CCAAGTGGGC 
CATTACGAGC TTGTTATGCC TCAAGCAAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NKRF ( NM_017544.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060014.2 Homo sapiens NFKB repressing factor (NKRF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu11903
Clone ID Related Accession (Same CDS sequence)	XM_011531365.1 , NM_017544.3 , NM_017544.4 , NM_001173488.1 , XM_011531365.2
Accession Version	NM_017544.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-06
Organism	Homo sapiens(human)
Product	NF-kappa-B-repressing factor isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY208891.1 and AA845622.1. On Jun 2, 2019 this sequence version replaced NM_017544.3. Transcript Variant: This variant (2) differs in the 5' UTR and 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC047878.2, SRR1803611.258553.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAAAAA TTCTCCAAAT GGCTGAAGGT ATTGATATTG GGGAGATGCC TTCATATGAT 
CTGGTGCTGT CCAAACCTTC CAAAGGTCAA AAACGCCACC TCTCAACATG TGATGGTCAA 
AATCCTCCTA AAAAGCAAGC CGGTTCCAAA TTCCATGCGA GACCTCGTTT TGAGCCTGTA 
CATTTTGTAG CTAGTAGTTC AAAAGATGAA AGACAGGAAG ATCCTTATGG CCCTCAAACA 
AAAGAGGTAA ATGAACAAAC ACATTTTGCC AGCATGCCAA GAGACATCTA CCAAGATTAT 
ACTCAAGACT CTTTCAGTAT ACAAGATGGG AATTCTCAGT ATTGTGATTC ATCAGGATTC 
ATTCTCACAA AAGACCAGCC TGTAACAGCC AACATGTATT TTGACAGTGG GAACCCTGCC 
CCAAGCACCA CATCACAGCA GGCAAACTCT CAGTCAACTC CTGAGCCTTC ACCATCACAG 
ACATTTCCCG AGTCTGTGGT AGCCGAGAAG CAGTATTTTA TTGAAAAATT AACGGCGACA 
ATCTGGAAGA ACCTTTCTAA TCCAGAAATG ACTTCTGGAT CTGATAAAAT TAATTATACA 
TATATGTTAA CTCGTTGTAT TCAGGCGTGT AAGACAAATC CTGAGTATAT ATATGCTCCT 
TTAAAGGAAA TTCCTCCTGC CGACATCCCC AAAAATAAAA AACTTCTAAC TGATGGCTAT 
GCTTGTGAAG TTAGATGCCA AAATATCTAC TTAACTACAG GTTATGCTGG CAGCAAGAAT 
GGGTCCAGGG ATCGAGCTAC AGAGCTAGCT GTAAAACTCT TGCAGAAACG TATTGAAGTT 
AGAGTTGTCC GGCGGAAATT CAAGCATACA TTTGGAGAGG ACCTCGTGGT GTGTCAGATT 
GGCATGTCCT CCTATGAATT TCCTCCAGCT CTGAAGCCAC CAGAAGACCT GGTGGTGCTG 
GGTAAAGATG CTTCCGGGCA GCCAATTTTT AATGCTTCTG CCAAACACTG GACCAATTTT 
GTCATTACAG AAAATGCAAA TGATGCAATT GGTATCCTTA ACAATTCTGC CTCATTCAAC 
AAGATGTCAA TTGAATACAA ATATGAGATG ATGCCAAATC GCACATGGCG TTGTCGAGTG 
TTTTTACAAG ATCACTGCTT AGCTGAAGGT TATGGAACCA AGAAAACAAG TAAACATGCA 
GCTGCCGACG AGGCTTTGAA AATTCTTCAA AAAACACAGC CCACTTATCC ATCTGTCAAA 
AGTTCACAAT GCCATACAGG CTCTTCACCC AGAGGATCTG GAAAGAAGAA AGATATAAAG 
GATCTTGTAG TTTATGAGAA TTCTTCAAAT CCCGTGTGCA CGCTGAACGA CACAGCTCAG 
TTTAACCGAA TGACAGTTGA GTATGTCTAT GAAAGGATGA CAGGCCTCCG CTGGAAATGC 
AAAGTGATTC TAGAGAGTGA AGTAATTGCA GAAGCAGTTG GGGTGAAGAA AACTGTCAAA 
TATGAAGCTG CTGGGGAAGC TGTGAAAACC CTCAAAAAGA CCCAGCCAAC TGTCATTAAC 
AACTTGAAGA AAGGAGCTGT TGAAGATGTG ATTTCAAGAA ATGAAATTCA GGGCCGCTCA 
GCAGAGGAGG CTTACAAACA GCAAATCAAA GAAGATAATA TTGGAAATCA GCTGCTGAGA 
AAGATGGGTT GGACTGGTGG TGGTTTAGGT AAATCTGGTG AGGGCATACG GGAGCCTATC 
TCAGTGAAAG AGCAGCATAA GCGGGAAGGG CTTGGTCTGG ATGTAGAGAG GGTGAATAAA 
ATTGCCAAGA GAGATATTGA ACAGATCATC AGAAACTACG CCCGCTCCGA GAGCCACACA 
GATTTGACTT TCTCTAGAGA GCTGACTAAT GATGAACGGA AGCAAATACA TCAGATTGCC 
CAGAAGTATG GTCTTAAGAG TAAGTCTCAT GGGGTGGGCC ATGATAGGTA CCTAGTGGTA 
GGTAGAAAAA GACGGAAGGA AGACCTACTA GATCAGCTCA AACAGGAAGG CCAAGTGGGC 
CATTACGAGC TTGTTATGCC TCAAGCAAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060014.2
CDS	168..2240
Translation	MEKILQMAEGIDIGEMPSYDLVLSKPSKGQKRHLSTCDGQNPPKKQAGSKFHARPRFEPVHFVASSSKDERQEDPYGPQTKEVNEQTHFASMPRDIYQDYTQDSFSIQDGNSQYCDSSGFILTKDQPVTANMYFDSGNPAPSTTSQQANSQSTPEPSPSQTFPESVVAEKQYFIEKLTATIWKNLSNPEMTSGSDKINYTYMLTRCIQACKTNPEYIYAPLKEIPPADIPKNKKLLTDGYACEVRCQNIYLTTGYAGSKNGSRDRATELAVKLLQKRIEVRVVRRKFKHTFGEDLVVCQIGMSSYEFPPALKPPEDLVVLGKDASGQPIFNASAKHWTNFVITENANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQAN

Target ORF information:

RefSeq Version	NM_017544.4
Organism	Homo sapiens(human)
Definition	Homo sapiens NFKB repressing factor (NKRF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017544.4

ORF Insert Sequence:

ATGGAAAAAA TTCTCCAAAT GGCTGAAGGT ATTGATATTG GGGAGATGCC TTCATATGAT 
CTGGTGCTGT CCAAACCTTC CAAAGGTCAA AAACGCCACC TCTCAACATG TGATGGTCAA 
AATCCTCCTA AAAAGCAAGC CGGTTCCAAA TTCCATGCGA GACCTCGTTT TGAGCCTGTA 
CATTTTGTAG CTAGTAGTTC AAAAGATGAA AGACAGGAAG ATCCTTATGG CCCTCAAACA 
AAAGAGGTAA ATGAACAAAC ACATTTTGCC AGCATGCCAA GAGACATCTA CCAAGATTAT 
ACTCAAGACT CTTTCAGTAT ACAAGATGGG AATTCTCAGT ATTGTGATTC ATCAGGATTC 
ATTCTCACAA AAGACCAGCC TGTAACAGCC AACATGTATT TTGACAGTGG GAACCCTGCC 
CCAAGCACCA CATCACAGCA GGCAAACTCT CAGTCAACTC CTGAGCCTTC ACCATCACAG 
ACATTTCCCG AGTCTGTGGT AGCCGAGAAG CAGTATTTTA TTGAAAAATT AACGGCGACA 
ATCTGGAAGA ACCTTTCTAA TCCAGAAATG ACTTCTGGAT CTGATAAAAT TAATTATACA 
TATATGTTAA CTCGTTGTAT TCAGGCGTGT AAGACAAATC CTGAGTATAT ATATGCTCCT 
TTAAAGGAAA TTCCTCCTGC CGACATCCCC AAAAATAAAA AACTTCTAAC TGATGGCTAT 
GCTTGTGAAG TTAGATGCCA AAATATCTAC TTAACTACAG GTTATGCTGG CAGCAAGAAT 
GGGTCCAGGG ATCGAGCTAC AGAGCTAGCT GTAAAACTCT TGCAGAAACG TATTGAAGTT 
AGAGTTGTCC GGCGGAAATT CAAGCATACA TTTGGAGAGG ACCTCGTGGT GTGTCAGATT 
GGCATGTCCT CCTATGAATT TCCTCCAGCT CTGAAGCCAC CAGAAGACCT GGTGGTGCTG 
GGTAAAGATG CTTCCGGGCA GCCAATTTTT AATGCTTCTG CCAAACACTG GACCAATTTT 
GTCATTACAG AAAATGCAAA TGATGCAATT GGTATCCTTA ACAATTCTGC CTCATTCAAC 
AAGATGTCAA TTGAATACAA ATATGAGATG ATGCCAAATC GCACATGGCG TTGTCGAGTG 
TTTTTACAAG ATCACTGCTT AGCTGAAGGT TATGGAACCA AGAAAACAAG TAAACATGCA 
GCTGCCGACG AGGCTTTGAA AATTCTTCAA AAAACACAGC CCACTTATCC ATCTGTCAAA 
AGTTCACAAT GCCATACAGG CTCTTCACCC AGAGGATCTG GAAAGAAGAA AGATATAAAG 
GATCTTGTAG TTTATGAGAA TTCTTCAAAT CCCGTGTGCA CGCTGAACGA CACAGCTCAG 
TTTAACCGAA TGACAGTTGA GTATGTCTAT GAAAGGATGA CAGGCCTCCG CTGGAAATGC 
AAAGTGATTC TAGAGAGTGA AGTAATTGCA GAAGCAGTTG GGGTGAAGAA AACTGTCAAA 
TATGAAGCTG CTGGGGAAGC TGTGAAAACC CTCAAAAAGA CCCAGCCAAC TGTCATTAAC 
AACTTGAAGA AAGGAGCTGT TGAAGATGTG ATTTCAAGAA ATGAAATTCA GGGCCGCTCA 
GCAGAGGAGG CTTACAAACA GCAAATCAAA GAAGATAATA TTGGAAATCA GCTGCTGAGA 
AAGATGGGTT GGACTGGTGG TGGTTTAGGT AAATCTGGTG AGGGCATACG GGAGCCTATC 
TCAGTGAAAG AGCAGCATAA GCGGGAAGGG CTTGGTCTGG ATGTAGAGAG GGTGAATAAA 
ATTGCCAAGA GAGATATTGA ACAGATCATC AGAAACTACG CCCGCTCCGA GAGCCACACA 
GATTTGACTT TCTCTAGAGA GCTGACTAAT GATGAACGGA AGCAAATACA TCAGATTGCC 
CAGAAGTATG GTCTTAAGAG TAAGTCTCAT GGGGTGGGCC ATGATAGGTA CCTAGTGGTA 
GGTAGAAAAA GACGGAAGGA AGACCTACTA GATCAGCTCA AACAGGAAGG CCAAGTGGGC 
CATTACGAGC TTGTTATGCC TCAAGCAAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NKRF ( NM_001173488.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166959.1 Homo sapiens NFKB repressing factor (NKRF), transcript variant 3, mRNA.

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CloneID	OHu11903
Clone ID Related Accession (Same CDS sequence)	XM_011531365.1 , NM_017544.3 , NM_017544.4 , NM_001173488.1 , XM_011531365.2
Accession Version	NM_001173488.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-05
Organism	Homo sapiens(human)
Product	NF-kappa-B-repressing factor isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from EF216688.1, AC004913.2, BC047878.2 and AA845622.1. Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region, and initiates translation at a downstream start codon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus, compared to isoform 1. Variants 2 and 3 encode the same isoform (2). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AJ011812.2, SRR1803611.69127.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAAAAA TTCTCCAAAT GGCTGAAGGT ATTGATATTG GGGAGATGCC TTCATATGAT 
CTGGTGCTGT CCAAACCTTC CAAAGGTCAA AAACGCCACC TCTCAACATG TGATGGTCAA 
AATCCTCCTA AAAAGCAAGC CGGTTCCAAA TTCCATGCGA GACCTCGTTT TGAGCCTGTA 
CATTTTGTAG CTAGTAGTTC AAAAGATGAA AGACAGGAAG ATCCTTATGG CCCTCAAACA 
AAAGAGGTAA ATGAACAAAC ACATTTTGCC AGCATGCCAA GAGACATCTA CCAAGATTAT 
ACTCAAGACT CTTTCAGTAT ACAAGATGGG AATTCTCAGT ATTGTGATTC ATCAGGATTC 
ATTCTCACAA AAGACCAGCC TGTAACAGCC AACATGTATT TTGACAGTGG GAACCCTGCC 
CCAAGCACCA CATCACAGCA GGCAAACTCT CAGTCAACTC CTGAGCCTTC ACCATCACAG 
ACATTTCCCG AGTCTGTGGT AGCCGAGAAG CAGTATTTTA TTGAAAAATT AACGGCGACA 
ATCTGGAAGA ACCTTTCTAA TCCAGAAATG ACTTCTGGAT CTGATAAAAT TAATTATACA 
TATATGTTAA CTCGTTGTAT TCAGGCGTGT AAGACAAATC CTGAGTATAT ATATGCTCCT 
TTAAAGGAAA TTCCTCCTGC CGACATCCCC AAAAATAAAA AACTTCTAAC TGATGGCTAT 
GCTTGTGAAG TTAGATGCCA AAATATCTAC TTAACTACAG GTTATGCTGG CAGCAAGAAT 
GGGTCCAGGG ATCGAGCTAC AGAGCTAGCT GTAAAACTCT TGCAGAAACG TATTGAAGTT 
AGAGTTGTCC GGCGGAAATT CAAGCATACA TTTGGAGAGG ACCTCGTGGT GTGTCAGATT 
GGCATGTCCT CCTATGAATT TCCTCCAGCT CTGAAGCCAC CAGAAGACCT GGTGGTGCTG 
GGTAAAGATG CTTCCGGGCA GCCAATTTTT AATGCTTCTG CCAAACACTG GACCAATTTT 
GTCATTACAG AAAATGCAAA TGATGCAATT GGTATCCTTA ACAATTCTGC CTCATTCAAC 
AAGATGTCAA TTGAATACAA ATATGAGATG ATGCCAAATC GCACATGGCG TTGTCGAGTG 
TTTTTACAAG ATCACTGCTT AGCTGAAGGT TATGGAACCA AGAAAACAAG TAAACATGCA 
GCTGCCGACG AGGCTTTGAA AATTCTTCAA AAAACACAGC CCACTTATCC ATCTGTCAAA 
AGTTCACAAT GCCATACAGG CTCTTCACCC AGAGGATCTG GAAAGAAGAA AGATATAAAG 
GATCTTGTAG TTTATGAGAA TTCTTCAAAT CCCGTGTGCA CGCTGAACGA CACAGCTCAG 
TTTAACCGAA TGACAGTTGA GTATGTCTAT GAAAGGATGA CAGGCCTCCG CTGGAAATGC 
AAAGTGATTC TAGAGAGTGA AGTAATTGCA GAAGCAGTTG GGGTGAAGAA AACTGTCAAA 
TATGAAGCTG CTGGGGAAGC TGTGAAAACC CTCAAAAAGA CCCAGCCAAC TGTCATTAAC 
AACTTGAAGA AAGGAGCTGT TGAAGATGTG ATTTCAAGAA ATGAAATTCA GGGCCGCTCA 
GCAGAGGAGG CTTACAAACA GCAAATCAAA GAAGATAATA TTGGAAATCA GCTGCTGAGA 
AAGATGGGTT GGACTGGTGG TGGTTTAGGT AAATCTGGTG AGGGCATACG GGAGCCTATC 
TCAGTGAAAG AGCAGCATAA GCGGGAAGGG CTTGGTCTGG ATGTAGAGAG GGTGAATAAA 
ATTGCCAAGA GAGATATTGA ACAGATCATC AGAAACTACG CCCGCTCCGA GAGCCACACA 
GATTTGACTT TCTCTAGAGA GCTGACTAAT GATGAACGGA AGCAAATACA TCAGATTGCC 
CAGAAGTATG GTCTTAAGAG TAAGTCTCAT GGGGTGGGCC ATGATAGGTA CCTAGTGGTA 
GGTAGAAAAA GACGGAAGGA AGACCTACTA GATCAGCTCA AACAGGAAGG CCAAGTGGGC 
CATTACGAGC TTGTTATGCC TCAAGCAAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166959.1
CDS	654..2726
Translation	MEKILQMAEGIDIGEMPSYDLVLSKPSKGQKRHLSTCDGQNPPKKQAGSKFHARPRFEPVHFVASSSKDERQEDPYGPQTKEVNEQTHFASMPRDIYQDYTQDSFSIQDGNSQYCDSSGFILTKDQPVTANMYFDSGNPAPSTTSQQANSQSTPEPSPSQTFPESVVAEKQYFIEKLTATIWKNLSNPEMTSGSDKINYTYMLTRCIQACKTNPEYIYAPLKEIPPADIPKNKKLLTDGYACEVRCQNIYLTTGYAGSKNGSRDRATELAVKLLQKRIEVRVVRRKFKHTFGEDLVVCQIGMSSYEFPPALKPPEDLVVLGKDASGQPIFNASAKHWTNFVITENANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQAN

Target ORF information:

RefSeq Version	NM_001173488.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NFKB repressing factor (NKRF), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001173488.1

ORF Insert Sequence:

ATGGAAAAAA TTCTCCAAAT GGCTGAAGGT ATTGATATTG GGGAGATGCC TTCATATGAT 
CTGGTGCTGT CCAAACCTTC CAAAGGTCAA AAACGCCACC TCTCAACATG TGATGGTCAA 
AATCCTCCTA AAAAGCAAGC CGGTTCCAAA TTCCATGCGA GACCTCGTTT TGAGCCTGTA 
CATTTTGTAG CTAGTAGTTC AAAAGATGAA AGACAGGAAG ATCCTTATGG CCCTCAAACA 
AAAGAGGTAA ATGAACAAAC ACATTTTGCC AGCATGCCAA GAGACATCTA CCAAGATTAT 
ACTCAAGACT CTTTCAGTAT ACAAGATGGG AATTCTCAGT ATTGTGATTC ATCAGGATTC 
ATTCTCACAA AAGACCAGCC TGTAACAGCC AACATGTATT TTGACAGTGG GAACCCTGCC 
CCAAGCACCA CATCACAGCA GGCAAACTCT CAGTCAACTC CTGAGCCTTC ACCATCACAG 
ACATTTCCCG AGTCTGTGGT AGCCGAGAAG CAGTATTTTA TTGAAAAATT AACGGCGACA 
ATCTGGAAGA ACCTTTCTAA TCCAGAAATG ACTTCTGGAT CTGATAAAAT TAATTATACA 
TATATGTTAA CTCGTTGTAT TCAGGCGTGT AAGACAAATC CTGAGTATAT ATATGCTCCT 
TTAAAGGAAA TTCCTCCTGC CGACATCCCC AAAAATAAAA AACTTCTAAC TGATGGCTAT 
GCTTGTGAAG TTAGATGCCA AAATATCTAC TTAACTACAG GTTATGCTGG CAGCAAGAAT 
GGGTCCAGGG ATCGAGCTAC AGAGCTAGCT GTAAAACTCT TGCAGAAACG TATTGAAGTT 
AGAGTTGTCC GGCGGAAATT CAAGCATACA TTTGGAGAGG ACCTCGTGGT GTGTCAGATT 
GGCATGTCCT CCTATGAATT TCCTCCAGCT CTGAAGCCAC CAGAAGACCT GGTGGTGCTG 
GGTAAAGATG CTTCCGGGCA GCCAATTTTT AATGCTTCTG CCAAACACTG GACCAATTTT 
GTCATTACAG AAAATGCAAA TGATGCAATT GGTATCCTTA ACAATTCTGC CTCATTCAAC 
AAGATGTCAA TTGAATACAA ATATGAGATG ATGCCAAATC GCACATGGCG TTGTCGAGTG 
TTTTTACAAG ATCACTGCTT AGCTGAAGGT TATGGAACCA AGAAAACAAG TAAACATGCA 
GCTGCCGACG AGGCTTTGAA AATTCTTCAA AAAACACAGC CCACTTATCC ATCTGTCAAA 
AGTTCACAAT GCCATACAGG CTCTTCACCC AGAGGATCTG GAAAGAAGAA AGATATAAAG 
GATCTTGTAG TTTATGAGAA TTCTTCAAAT CCCGTGTGCA CGCTGAACGA CACAGCTCAG 
TTTAACCGAA TGACAGTTGA GTATGTCTAT GAAAGGATGA CAGGCCTCCG CTGGAAATGC 
AAAGTGATTC TAGAGAGTGA AGTAATTGCA GAAGCAGTTG GGGTGAAGAA AACTGTCAAA 
TATGAAGCTG CTGGGGAAGC TGTGAAAACC CTCAAAAAGA CCCAGCCAAC TGTCATTAAC 
AACTTGAAGA AAGGAGCTGT TGAAGATGTG ATTTCAAGAA ATGAAATTCA GGGCCGCTCA 
GCAGAGGAGG CTTACAAACA GCAAATCAAA GAAGATAATA TTGGAAATCA GCTGCTGAGA 
AAGATGGGTT GGACTGGTGG TGGTTTAGGT AAATCTGGTG AGGGCATACG GGAGCCTATC 
TCAGTGAAAG AGCAGCATAA GCGGGAAGGG CTTGGTCTGG ATGTAGAGAG GGTGAATAAA 
ATTGCCAAGA GAGATATTGA ACAGATCATC AGAAACTACG CCCGCTCCGA GAGCCACACA 
GATTTGACTT TCTCTAGAGA GCTGACTAAT GATGAACGGA AGCAAATACA TCAGATTGCC 
CAGAAGTATG GTCTTAAGAG TAAGTCTCAT GGGGTGGGCC ATGATAGGTA CCTAGTGGTA 
GGTAGAAAAA GACGGAAGGA AGACCTACTA GATCAGCTCA AACAGGAAGG CCAAGTGGGC 
CATTACGAGC TTGTTATGCC TCAAGCAAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NKRF ( XM_011531365.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529667.1 Homo sapiens NFKB repressing factor (NKRF), transcript variant X1, mRNA.

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CloneID	OHu11903
Clone ID Related Accession (Same CDS sequence)	XM_011531365.1 , NM_017544.3 , NM_017544.4 , NM_001173488.1 , XM_011531365.2
Accession Version	XM_011531365.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-03-11
Organism	Homo sapiens(Human)
Product	NF-kappa-B-repressing factor isoform X1
Comment

ATGGAAAAAA TTCTCCAAAT GGCTGAAGGT ATTGATATTG GGGAGATGCC TTCATATGAT 
CTGGTGCTGT CCAAACCTTC CAAAGGTCAA AAACGCCACC TCTCAACATG TGATGGTCAA 
AATCCTCCTA AAAAGCAAGC CGGTTCCAAA TTCCATGCGA GACCTCGTTT TGAGCCTGTA 
CATTTTGTAG CTAGTAGTTC AAAAGATGAA AGACAGGAAG ATCCTTATGG CCCTCAAACA 
AAAGAGGTAA ATGAACAAAC ACATTTTGCC AGCATGCCAA GAGACATCTA CCAAGATTAT 
ACTCAAGACT CTTTCAGTAT ACAAGATGGG AATTCTCAGT ATTGTGATTC ATCAGGATTC 
ATTCTCACAA AAGACCAGCC TGTAACAGCC AACATGTATT TTGACAGTGG GAACCCTGCC 
CCAAGCACCA CATCACAGCA GGCAAACTCT CAGTCAACTC CTGAGCCTTC ACCATCACAG 
ACATTTCCCG AGTCTGTGGT AGCCGAGAAG CAGTATTTTA TTGAAAAATT AACGGCGACA 
ATCTGGAAGA ACCTTTCTAA TCCAGAAATG ACTTCTGGAT CTGATAAAAT TAATTATACA 
TATATGTTAA CTCGTTGTAT TCAGGCGTGT AAGACAAATC CTGAGTATAT ATATGCTCCT 
TTAAAGGAAA TTCCTCCTGC CGACATCCCC AAAAATAAAA AACTTCTAAC TGATGGCTAT 
GCTTGTGAAG TTAGATGCCA AAATATCTAC TTAACTACAG GTTATGCTGG CAGCAAGAAT 
GGGTCCAGGG ATCGAGCTAC AGAGCTAGCT GTAAAACTCT TGCAGAAACG TATTGAAGTT 
AGAGTTGTCC GGCGGAAATT CAAGCATACA TTTGGAGAGG ACCTCGTGGT GTGTCAGATT 
GGCATGTCCT CCTATGAATT TCCTCCAGCT CTGAAGCCAC CAGAAGACCT GGTGGTGCTG 
GGTAAAGATG CTTCCGGGCA GCCAATTTTT AATGCTTCTG CCAAACACTG GACCAATTTT 
GTCATTACAG AAAATGCAAA TGATGCAATT GGTATCCTTA ACAATTCTGC CTCATTCAAC 
AAGATGTCAA TTGAATACAA ATATGAGATG ATGCCAAATC GCACATGGCG TTGTCGAGTG 
TTTTTACAAG ATCACTGCTT AGCTGAAGGT TATGGAACCA AGAAAACAAG TAAACATGCA 
GCTGCCGACG AGGCTTTGAA AATTCTTCAA AAAACACAGC CCACTTATCC ATCTGTCAAA 
AGTTCACAAT GCCATACAGG CTCTTCACCC AGAGGATCTG GAAAGAAGAA AGATATAAAG 
GATCTTGTAG TTTATGAGAA TTCTTCAAAT CCCGTGTGCA CGCTGAACGA CACAGCTCAG 
TTTAACCGAA TGACAGTTGA GTATGTCTAT GAAAGGATGA CAGGCCTCCG CTGGAAATGC 
AAAGTGATTC TAGAGAGTGA AGTAATTGCA GAAGCAGTTG GGGTGAAGAA AACTGTCAAA 
TATGAAGCTG CTGGGGAAGC TGTGAAAACC CTCAAAAAGA CCCAGCCAAC TGTCATTAAC 
AACTTGAAGA AAGGAGCTGT TGAAGATGTG ATTTCAAGAA ATGAAATTCA GGGCCGCTCA 
GCAGAGGAGG CTTACAAACA GCAAATCAAA GAAGATAATA TTGGAAATCA GCTGCTGAGA 
AAGATGGGTT GGACTGGTGG TGGTTTAGGT AAATCTGGTG AGGGCATACG GGAGCCTATC 
TCAGTGAAAG AGCAGCATAA GCGGGAAGGG CTTGGTCTGG ATGTAGAGAG GGTGAATAAA 
ATTGCCAAGA GAGATATTGA ACAGATCATC AGAAACTACG CCCGCTCCGA GAGCCACACA 
GATTTGACTT TCTCTAGAGA GCTGACTAAT GATGAACGGA AGCAAATACA TCAGATTGCC 
CAGAAGTATG GTCTTAAGAG TAAGTCTCAT GGGGTGGGCC ATGATAGGTA CCTAGTGGTA 
GGTAGAAAAA GACGGAAGGA AGACCTACTA GATCAGCTCA AACAGGAAGG CCAAGTGGGC 
CATTACGAGC TTGTTATGCC TCAAGCAAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

XP_011529667.1

Translation

MEKILQMAEGIDIGEMPSYDLVLSKPSKGQKRHLSTCDGQNPPKKQAGSKFHARPRFEPVHFVASSSKDERQEDPYGPQTKEVNEQTHFASMPRDIYQDYTQDSFSIQDGNSQYCDSSGFILTKDQPVTANMYFDSGNPAPSTTSQQANSQSTPEPSPSQTFPESVVAEKQYFIEKLTATIWKNLSNPEMTSGSDKINYTYMLTRCIQACKTNPEYIYAPLKEIPPADIPKNKKLLTDGYACEVRCQNIYLTTGYAGSKNGSRDRATELAVKLLQKRIEVRVVRRKFKHTFGEDLVVCQIGMSSYEFPPALKPPEDLVVLGKDASGQPIFNASAKHWTNFVITENANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQAN

Target ORF information:

RefSeq Version	XM_011531365.1
Organism	Homo sapiens(Human)
Definition	Homo sapiens NFKB repressing factor (NKRF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531365.1

ORF Insert Sequence:

ATGGAAAAAA TTCTCCAAAT GGCTGAAGGT ATTGATATTG GGGAGATGCC TTCATATGAT 
CTGGTGCTGT CCAAACCTTC CAAAGGTCAA AAACGCCACC TCTCAACATG TGATGGTCAA 
AATCCTCCTA AAAAGCAAGC CGGTTCCAAA TTCCATGCGA GACCTCGTTT TGAGCCTGTA 
CATTTTGTAG CTAGTAGTTC AAAAGATGAA AGACAGGAAG ATCCTTATGG CCCTCAAACA 
AAAGAGGTAA ATGAACAAAC ACATTTTGCC AGCATGCCAA GAGACATCTA CCAAGATTAT 
ACTCAAGACT CTTTCAGTAT ACAAGATGGG AATTCTCAGT ATTGTGATTC ATCAGGATTC 
ATTCTCACAA AAGACCAGCC TGTAACAGCC AACATGTATT TTGACAGTGG GAACCCTGCC 
CCAAGCACCA CATCACAGCA GGCAAACTCT CAGTCAACTC CTGAGCCTTC ACCATCACAG 
ACATTTCCCG AGTCTGTGGT AGCCGAGAAG CAGTATTTTA TTGAAAAATT AACGGCGACA 
ATCTGGAAGA ACCTTTCTAA TCCAGAAATG ACTTCTGGAT CTGATAAAAT TAATTATACA 
TATATGTTAA CTCGTTGTAT TCAGGCGTGT AAGACAAATC CTGAGTATAT ATATGCTCCT 
TTAAAGGAAA TTCCTCCTGC CGACATCCCC AAAAATAAAA AACTTCTAAC TGATGGCTAT 
GCTTGTGAAG TTAGATGCCA AAATATCTAC TTAACTACAG GTTATGCTGG CAGCAAGAAT 
GGGTCCAGGG ATCGAGCTAC AGAGCTAGCT GTAAAACTCT TGCAGAAACG TATTGAAGTT 
AGAGTTGTCC GGCGGAAATT CAAGCATACA TTTGGAGAGG ACCTCGTGGT GTGTCAGATT 
GGCATGTCCT CCTATGAATT TCCTCCAGCT CTGAAGCCAC CAGAAGACCT GGTGGTGCTG 
GGTAAAGATG CTTCCGGGCA GCCAATTTTT AATGCTTCTG CCAAACACTG GACCAATTTT 
GTCATTACAG AAAATGCAAA TGATGCAATT GGTATCCTTA ACAATTCTGC CTCATTCAAC 
AAGATGTCAA TTGAATACAA ATATGAGATG ATGCCAAATC GCACATGGCG TTGTCGAGTG 
TTTTTACAAG ATCACTGCTT AGCTGAAGGT TATGGAACCA AGAAAACAAG TAAACATGCA 
GCTGCCGACG AGGCTTTGAA AATTCTTCAA AAAACACAGC CCACTTATCC ATCTGTCAAA 
AGTTCACAAT GCCATACAGG CTCTTCACCC AGAGGATCTG GAAAGAAGAA AGATATAAAG 
GATCTTGTAG TTTATGAGAA TTCTTCAAAT CCCGTGTGCA CGCTGAACGA CACAGCTCAG 
TTTAACCGAA TGACAGTTGA GTATGTCTAT GAAAGGATGA CAGGCCTCCG CTGGAAATGC 
AAAGTGATTC TAGAGAGTGA AGTAATTGCA GAAGCAGTTG GGGTGAAGAA AACTGTCAAA 
TATGAAGCTG CTGGGGAAGC TGTGAAAACC CTCAAAAAGA CCCAGCCAAC TGTCATTAAC 
AACTTGAAGA AAGGAGCTGT TGAAGATGTG ATTTCAAGAA ATGAAATTCA GGGCCGCTCA 
GCAGAGGAGG CTTACAAACA GCAAATCAAA GAAGATAATA TTGGAAATCA GCTGCTGAGA 
AAGATGGGTT GGACTGGTGG TGGTTTAGGT AAATCTGGTG AGGGCATACG GGAGCCTATC 
TCAGTGAAAG AGCAGCATAA GCGGGAAGGG CTTGGTCTGG ATGTAGAGAG GGTGAATAAA 
ATTGCCAAGA GAGATATTGA ACAGATCATC AGAAACTACG CCCGCTCCGA GAGCCACACA 
GATTTGACTT TCTCTAGAGA GCTGACTAAT GATGAACGGA AGCAAATACA TCAGATTGCC 
CAGAAGTATG GTCTTAAGAG TAAGTCTCAT GGGGTGGGCC ATGATAGGTA CCTAGTGGTA 
GGTAGAAAAA GACGGAAGGA AGACCTACTA GATCAGCTCA AACAGGAAGG CCAAGTGGGC 
CATTACGAGC TTGTTATGCC TCAAGCAAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NKRF ( XM_011531365.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011529667.1 Homo sapiens NFKB repressing factor (NKRF), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu11903
Clone ID Related Accession (Same CDS sequence)	XM_011531365.1 , NM_017544.3 , NM_017544.4 , NM_001173488.1 , XM_011531365.2
Accession Version	XM_011531365.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	NF-kappa-B-repressing factor isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000023.11) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011531365.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAAAA TTCTCCAAAT GGCTGAAGGT ATTGATATTG GGGAGATGCC TTCATATGAT 
CTGGTGCTGT CCAAACCTTC CAAAGGTCAA AAACGCCACC TCTCAACATG TGATGGTCAA 
AATCCTCCTA AAAAGCAAGC CGGTTCCAAA TTCCATGCGA GACCTCGTTT TGAGCCTGTA 
CATTTTGTAG CTAGTAGTTC AAAAGATGAA AGACAGGAAG ATCCTTATGG CCCTCAAACA 
AAAGAGGTAA ATGAACAAAC ACATTTTGCC AGCATGCCAA GAGACATCTA CCAAGATTAT 
ACTCAAGACT CTTTCAGTAT ACAAGATGGG AATTCTCAGT ATTGTGATTC ATCAGGATTC 
ATTCTCACAA AAGACCAGCC TGTAACAGCC AACATGTATT TTGACAGTGG GAACCCTGCC 
CCAAGCACCA CATCACAGCA GGCAAACTCT CAGTCAACTC CTGAGCCTTC ACCATCACAG 
ACATTTCCCG AGTCTGTGGT AGCCGAGAAG CAGTATTTTA TTGAAAAATT AACGGCGACA 
ATCTGGAAGA ACCTTTCTAA TCCAGAAATG ACTTCTGGAT CTGATAAAAT TAATTATACA 
TATATGTTAA CTCGTTGTAT TCAGGCGTGT AAGACAAATC CTGAGTATAT ATATGCTCCT 
TTAAAGGAAA TTCCTCCTGC CGACATCCCC AAAAATAAAA AACTTCTAAC TGATGGCTAT 
GCTTGTGAAG TTAGATGCCA AAATATCTAC TTAACTACAG GTTATGCTGG CAGCAAGAAT 
GGGTCCAGGG ATCGAGCTAC AGAGCTAGCT GTAAAACTCT TGCAGAAACG TATTGAAGTT 
AGAGTTGTCC GGCGGAAATT CAAGCATACA TTTGGAGAGG ACCTCGTGGT GTGTCAGATT 
GGCATGTCCT CCTATGAATT TCCTCCAGCT CTGAAGCCAC CAGAAGACCT GGTGGTGCTG 
GGTAAAGATG CTTCCGGGCA GCCAATTTTT AATGCTTCTG CCAAACACTG GACCAATTTT 
GTCATTACAG AAAATGCAAA TGATGCAATT GGTATCCTTA ACAATTCTGC CTCATTCAAC 
AAGATGTCAA TTGAATACAA ATATGAGATG ATGCCAAATC GCACATGGCG TTGTCGAGTG 
TTTTTACAAG ATCACTGCTT AGCTGAAGGT TATGGAACCA AGAAAACAAG TAAACATGCA 
GCTGCCGACG AGGCTTTGAA AATTCTTCAA AAAACACAGC CCACTTATCC ATCTGTCAAA 
AGTTCACAAT GCCATACAGG CTCTTCACCC AGAGGATCTG GAAAGAAGAA AGATATAAAG 
GATCTTGTAG TTTATGAGAA TTCTTCAAAT CCCGTGTGCA CGCTGAACGA CACAGCTCAG 
TTTAACCGAA TGACAGTTGA GTATGTCTAT GAAAGGATGA CAGGCCTCCG CTGGAAATGC 
AAAGTGATTC TAGAGAGTGA AGTAATTGCA GAAGCAGTTG GGGTGAAGAA AACTGTCAAA 
TATGAAGCTG CTGGGGAAGC TGTGAAAACC CTCAAAAAGA CCCAGCCAAC TGTCATTAAC 
AACTTGAAGA AAGGAGCTGT TGAAGATGTG ATTTCAAGAA ATGAAATTCA GGGCCGCTCA 
GCAGAGGAGG CTTACAAACA GCAAATCAAA GAAGATAATA TTGGAAATCA GCTGCTGAGA 
AAGATGGGTT GGACTGGTGG TGGTTTAGGT AAATCTGGTG AGGGCATACG GGAGCCTATC 
TCAGTGAAAG AGCAGCATAA GCGGGAAGGG CTTGGTCTGG ATGTAGAGAG GGTGAATAAA 
ATTGCCAAGA GAGATATTGA ACAGATCATC AGAAACTACG CCCGCTCCGA GAGCCACACA 
GATTTGACTT TCTCTAGAGA GCTGACTAAT GATGAACGGA AGCAAATACA TCAGATTGCC 
CAGAAGTATG GTCTTAAGAG TAAGTCTCAT GGGGTGGGCC ATGATAGGTA CCTAGTGGTA 
GGTAGAAAAA GACGGAAGGA AGACCTACTA GATCAGCTCA AACAGGAAGG CCAAGTGGGC 
CATTACGAGC TTGTTATGCC TCAAGCAAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011529667.1
CDS	800..2872
Translation	MEKILQMAEGIDIGEMPSYDLVLSKPSKGQKRHLSTCDGQNPPKKQAGSKFHARPRFEPVHFVASSSKDERQEDPYGPQTKEVNEQTHFASMPRDIYQDYTQDSFSIQDGNSQYCDSSGFILTKDQPVTANMYFDSGNPAPSTTSQQANSQSTPEPSPSQTFPESVVAEKQYFIEKLTATIWKNLSNPEMTSGSDKINYTYMLTRCIQACKTNPEYIYAPLKEIPPADIPKNKKLLTDGYACEVRCQNIYLTTGYAGSKNGSRDRATELAVKLLQKRIEVRVVRRKFKHTFGEDLVVCQIGMSSYEFPPALKPPEDLVVLGKDASGQPIFNASAKHWTNFVITENANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQAN

Target ORF information:

RefSeq Version	XM_011531365.2
Organism	Homo sapiens(human)
Definition	Homo sapiens NFKB repressing factor (NKRF), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011531365.2

ORF Insert Sequence:

ATGGAAAAAA TTCTCCAAAT GGCTGAAGGT ATTGATATTG GGGAGATGCC TTCATATGAT 
CTGGTGCTGT CCAAACCTTC CAAAGGTCAA AAACGCCACC TCTCAACATG TGATGGTCAA 
AATCCTCCTA AAAAGCAAGC CGGTTCCAAA TTCCATGCGA GACCTCGTTT TGAGCCTGTA 
CATTTTGTAG CTAGTAGTTC AAAAGATGAA AGACAGGAAG ATCCTTATGG CCCTCAAACA 
AAAGAGGTAA ATGAACAAAC ACATTTTGCC AGCATGCCAA GAGACATCTA CCAAGATTAT 
ACTCAAGACT CTTTCAGTAT ACAAGATGGG AATTCTCAGT ATTGTGATTC ATCAGGATTC 
ATTCTCACAA AAGACCAGCC TGTAACAGCC AACATGTATT TTGACAGTGG GAACCCTGCC 
CCAAGCACCA CATCACAGCA GGCAAACTCT CAGTCAACTC CTGAGCCTTC ACCATCACAG 
ACATTTCCCG AGTCTGTGGT AGCCGAGAAG CAGTATTTTA TTGAAAAATT AACGGCGACA 
ATCTGGAAGA ACCTTTCTAA TCCAGAAATG ACTTCTGGAT CTGATAAAAT TAATTATACA 
TATATGTTAA CTCGTTGTAT TCAGGCGTGT AAGACAAATC CTGAGTATAT ATATGCTCCT 
TTAAAGGAAA TTCCTCCTGC CGACATCCCC AAAAATAAAA AACTTCTAAC TGATGGCTAT 
GCTTGTGAAG TTAGATGCCA AAATATCTAC TTAACTACAG GTTATGCTGG CAGCAAGAAT 
GGGTCCAGGG ATCGAGCTAC AGAGCTAGCT GTAAAACTCT TGCAGAAACG TATTGAAGTT 
AGAGTTGTCC GGCGGAAATT CAAGCATACA TTTGGAGAGG ACCTCGTGGT GTGTCAGATT 
GGCATGTCCT CCTATGAATT TCCTCCAGCT CTGAAGCCAC CAGAAGACCT GGTGGTGCTG 
GGTAAAGATG CTTCCGGGCA GCCAATTTTT AATGCTTCTG CCAAACACTG GACCAATTTT 
GTCATTACAG AAAATGCAAA TGATGCAATT GGTATCCTTA ACAATTCTGC CTCATTCAAC 
AAGATGTCAA TTGAATACAA ATATGAGATG ATGCCAAATC GCACATGGCG TTGTCGAGTG 
TTTTTACAAG ATCACTGCTT AGCTGAAGGT TATGGAACCA AGAAAACAAG TAAACATGCA 
GCTGCCGACG AGGCTTTGAA AATTCTTCAA AAAACACAGC CCACTTATCC ATCTGTCAAA 
AGTTCACAAT GCCATACAGG CTCTTCACCC AGAGGATCTG GAAAGAAGAA AGATATAAAG 
GATCTTGTAG TTTATGAGAA TTCTTCAAAT CCCGTGTGCA CGCTGAACGA CACAGCTCAG 
TTTAACCGAA TGACAGTTGA GTATGTCTAT GAAAGGATGA CAGGCCTCCG CTGGAAATGC 
AAAGTGATTC TAGAGAGTGA AGTAATTGCA GAAGCAGTTG GGGTGAAGAA AACTGTCAAA 
TATGAAGCTG CTGGGGAAGC TGTGAAAACC CTCAAAAAGA CCCAGCCAAC TGTCATTAAC 
AACTTGAAGA AAGGAGCTGT TGAAGATGTG ATTTCAAGAA ATGAAATTCA GGGCCGCTCA 
GCAGAGGAGG CTTACAAACA GCAAATCAAA GAAGATAATA TTGGAAATCA GCTGCTGAGA 
AAGATGGGTT GGACTGGTGG TGGTTTAGGT AAATCTGGTG AGGGCATACG GGAGCCTATC 
TCAGTGAAAG AGCAGCATAA GCGGGAAGGG CTTGGTCTGG ATGTAGAGAG GGTGAATAAA 
ATTGCCAAGA GAGATATTGA ACAGATCATC AGAAACTACG CCCGCTCCGA GAGCCACACA 
GATTTGACTT TCTCTAGAGA GCTGACTAAT GATGAACGGA AGCAAATACA TCAGATTGCC 
CAGAAGTATG GTCTTAAGAG TAAGTCTCAT GGGGTGGGCC ATGATAGGTA CCTAGTGGTA 
GGTAGAAAAA GACGGAAGGA AGACCTACTA GATCAGCTCA AACAGGAAGG CCAAGTGGGC 
CATTACGAGC TTGTTATGCC TCAAGCAAAT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NKRF ( NM_001173487.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001166958.1 Homo sapiens NFKB repressing factor (NKRF), transcript variant 1, mRNA.

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CloneID	OHu04603
Clone ID Related Accession (Same CDS sequence)	NM_001173487.1
Accession Version	NM_001173487.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2118bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-04
Organism	Homo sapiens(human)
Product	NF-kappa-B-repressing factor isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY208891.1, AL539002.3, BC047878.2 and AA845622.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.63837.1, AL539002.3 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2142670, SAMEA2159080 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGGCTTTA TGTTACCTCT CATCTTCAGG TACAGCCCAC GCTTGATGGA AAAAATTCTC 
CAAATGGCTG AAGGTATTGA TATTGGGGAG ATGCCTTCAT ATGATCTGGT GCTGTCCAAA 
CCTTCCAAAG GTCAAAAACG CCACCTCTCA ACATGTGATG GTCAAAATCC TCCTAAAAAG 
CAAGCCGGTT CCAAATTCCA TGCGAGACCT CGTTTTGAGC CTGTACATTT TGTAGCTAGT 
AGTTCAAAAG ATGAAAGACA GGAAGATCCT TATGGCCCTC AAACAAAAGA GGTAAATGAA 
CAAACACATT TTGCCAGCAT GCCAAGAGAC ATCTACCAAG ATTATACTCA AGACTCTTTC 
AGTATACAAG ATGGGAATTC TCAGTATTGT GATTCATCAG GATTCATTCT CACAAAAGAC 
CAGCCTGTAA CAGCCAACAT GTATTTTGAC AGTGGGAACC CTGCCCCAAG CACCACATCA 
CAGCAGGCAA ACTCTCAGTC AACTCCTGAG CCTTCACCAT CACAGACATT TCCCGAGTCT 
GTGGTAGCCG AGAAGCAGTA TTTTATTGAA AAATTAACGG CGACAATCTG GAAGAACCTT 
TCTAATCCAG AAATGACTTC TGGATCTGAT AAAATTAATT ATACATATAT GTTAACTCGT 
TGTATTCAGG CGTGTAAGAC AAATCCTGAG TATATATATG CTCCTTTAAA GGAAATTCCT 
CCTGCCGACA TCCCCAAAAA TAAAAAACTT CTAACTGATG GCTATGCTTG TGAAGTTAGA 
TGCCAAAATA TCTACTTAAC TACAGGTTAT GCTGGCAGCA AGAATGGGTC CAGGGATCGA 
GCTACAGAGC TAGCTGTAAA ACTCTTGCAG AAACGTATTG AAGTTAGAGT TGTCCGGCGG 
AAATTCAAGC ATACATTTGG AGAGGACCTC GTGGTGTGTC AGATTGGCAT GTCCTCCTAT 
GAATTTCCTC CAGCTCTGAA GCCACCAGAA GACCTGGTGG TGCTGGGTAA AGATGCTTCC 
GGGCAGCCAA TTTTTAATGC TTCTGCCAAA CACTGGACCA ATTTTGTCAT TACAGAAAAT 
GCAAATGATG CAATTGGTAT CCTTAACAAT TCTGCCTCAT TCAACAAGAT GTCAATTGAA 
TACAAATATG AGATGATGCC AAATCGCACA TGGCGTTGTC GAGTGTTTTT ACAAGATCAC 
TGCTTAGCTG AAGGTTATGG AACCAAGAAA ACAAGTAAAC ATGCAGCTGC CGACGAGGCT 
TTGAAAATTC TTCAAAAAAC ACAGCCCACT TATCCATCTG TCAAAAGTTC ACAATGCCAT 
ACAGGCTCTT CACCCAGAGG ATCTGGAAAG AAGAAAGATA TAAAGGATCT TGTAGTTTAT 
GAGAATTCTT CAAATCCCGT GTGCACGCTG AACGACACAG CTCAGTTTAA CCGAATGACA 
GTTGAGTATG TCTATGAAAG GATGACAGGC CTCCGCTGGA AATGCAAAGT GATTCTAGAG 
AGTGAAGTAA TTGCAGAAGC AGTTGGGGTG AAGAAAACTG TCAAATATGA AGCTGCTGGG 
GAAGCTGTGA AAACCCTCAA AAAGACCCAG CCAACTGTCA TTAACAACTT GAAGAAAGGA 
GCTGTTGAAG ATGTGATTTC AAGAAATGAA ATTCAGGGCC GCTCAGCAGA GGAGGCTTAC 
AAACAGCAAA TCAAAGAAGA TAATATTGGA AATCAGCTGC TGAGAAAGAT GGGTTGGACT 
GGTGGTGGTT TAGGTAAATC TGGTGAGGGC ATACGGGAGC CTATCTCAGT GAAAGAGCAG 
CATAAGCGGG AAGGGCTTGG TCTGGATGTA GAGAGGGTGA ATAAAATTGC CAAGAGAGAT 
ATTGAACAGA TCATCAGAAA CTACGCCCGC TCCGAGAGCC ACACAGATTT GACTTTCTCT 
AGAGAGCTGA CTAATGATGA ACGGAAGCAA ATACATCAGA TTGCCCAGAA GTATGGTCTT 
AAGAGTAAGT CTCATGGGGT GGGCCATGAT AGGTACCTAG TGGTAGGTAG AAAAAGACGG 
AAGGAAGACC TACTAGATCA GCTCAAACAG GAAGGCCAAG TGGGCCATTA CGAGCTTGTT 
ATGCCTCAAG CAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001166958.1
CDS	223..2340
Translation	MGFMLPLIFRYSPRLMEKILQMAEGIDIGEMPSYDLVLSKPSKGQKRHLSTCDGQNPPKKQAGSKFHARPRFEPVHFVASSSKDERQEDPYGPQTKEVNEQTHFASMPRDIYQDYTQDSFSIQDGNSQYCDSSGFILTKDQPVTANMYFDSGNPAPSTTSQQANSQSTPEPSPSQTFPESVVAEKQYFIEKLTATIWKNLSNPEMTSGSDKINYTYMLTRCIQACKTNPEYIYAPLKEIPPADIPKNKKLLTDGYACEVRCQNIYLTTGYAGSKNGSRDRATELAVKLLQKRIEVRVVRRKFKHTFGEDLVVCQIGMSSYEFPPALKPPEDLVVLGKDASGQPIFNASAKHWTNFVITENANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQAN

Target ORF information:

RefSeq Version	NM_001173487.1
Organism	Homo sapiens(human)
Definition	Homo sapiens NFKB repressing factor (NKRF), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001173487.1

ORF Insert Sequence:

ATGGGCTTTA TGTTACCTCT CATCTTCAGG TACAGCCCAC GCTTGATGGA AAAAATTCTC 
CAAATGGCTG AAGGTATTGA TATTGGGGAG ATGCCTTCAT ATGATCTGGT GCTGTCCAAA 
CCTTCCAAAG GTCAAAAACG CCACCTCTCA ACATGTGATG GTCAAAATCC TCCTAAAAAG 
CAAGCCGGTT CCAAATTCCA TGCGAGACCT CGTTTTGAGC CTGTACATTT TGTAGCTAGT 
AGTTCAAAAG ATGAAAGACA GGAAGATCCT TATGGCCCTC AAACAAAAGA GGTAAATGAA 
CAAACACATT TTGCCAGCAT GCCAAGAGAC ATCTACCAAG ATTATACTCA AGACTCTTTC 
AGTATACAAG ATGGGAATTC TCAGTATTGT GATTCATCAG GATTCATTCT CACAAAAGAC 
CAGCCTGTAA CAGCCAACAT GTATTTTGAC AGTGGGAACC CTGCCCCAAG CACCACATCA 
CAGCAGGCAA ACTCTCAGTC AACTCCTGAG CCTTCACCAT CACAGACATT TCCCGAGTCT 
GTGGTAGCCG AGAAGCAGTA TTTTATTGAA AAATTAACGG CGACAATCTG GAAGAACCTT 
TCTAATCCAG AAATGACTTC TGGATCTGAT AAAATTAATT ATACATATAT GTTAACTCGT 
TGTATTCAGG CGTGTAAGAC AAATCCTGAG TATATATATG CTCCTTTAAA GGAAATTCCT 
CCTGCCGACA TCCCCAAAAA TAAAAAACTT CTAACTGATG GCTATGCTTG TGAAGTTAGA 
TGCCAAAATA TCTACTTAAC TACAGGTTAT GCTGGCAGCA AGAATGGGTC CAGGGATCGA 
GCTACAGAGC TAGCTGTAAA ACTCTTGCAG AAACGTATTG AAGTTAGAGT TGTCCGGCGG 
AAATTCAAGC ATACATTTGG AGAGGACCTC GTGGTGTGTC AGATTGGCAT GTCCTCCTAT 
GAATTTCCTC CAGCTCTGAA GCCACCAGAA GACCTGGTGG TGCTGGGTAA AGATGCTTCC 
GGGCAGCCAA TTTTTAATGC TTCTGCCAAA CACTGGACCA ATTTTGTCAT TACAGAAAAT 
GCAAATGATG CAATTGGTAT CCTTAACAAT TCTGCCTCAT TCAACAAGAT GTCAATTGAA 
TACAAATATG AGATGATGCC AAATCGCACA TGGCGTTGTC GAGTGTTTTT ACAAGATCAC 
TGCTTAGCTG AAGGTTATGG AACCAAGAAA ACAAGTAAAC ATGCAGCTGC CGACGAGGCT 
TTGAAAATTC TTCAAAAAAC ACAGCCCACT TATCCATCTG TCAAAAGTTC ACAATGCCAT 
ACAGGCTCTT CACCCAGAGG ATCTGGAAAG AAGAAAGATA TAAAGGATCT TGTAGTTTAT 
GAGAATTCTT CAAATCCCGT GTGCACGCTG AACGACACAG CTCAGTTTAA CCGAATGACA 
GTTGAGTATG TCTATGAAAG GATGACAGGC CTCCGCTGGA AATGCAAAGT GATTCTAGAG 
AGTGAAGTAA TTGCAGAAGC AGTTGGGGTG AAGAAAACTG TCAAATATGA AGCTGCTGGG 
GAAGCTGTGA AAACCCTCAA AAAGACCCAG CCAACTGTCA TTAACAACTT GAAGAAAGGA 
GCTGTTGAAG ATGTGATTTC AAGAAATGAA ATTCAGGGCC GCTCAGCAGA GGAGGCTTAC 
AAACAGCAAA TCAAAGAAGA TAATATTGGA AATCAGCTGC TGAGAAAGAT GGGTTGGACT 
GGTGGTGGTT TAGGTAAATC TGGTGAGGGC ATACGGGAGC CTATCTCAGT GAAAGAGCAG 
CATAAGCGGG AAGGGCTTGG TCTGGATGTA GAGAGGGTGA ATAAAATTGC CAAGAGAGAT 
ATTGAACAGA TCATCAGAAA CTACGCCCGC TCCGAGAGCC ACACAGATTT GACTTTCTCT 
AGAGAGCTGA CTAATGATGA ACGGAAGCAA ATACATCAGA TTGCCCAGAA GTATGGTCTT 
AAGAGTAAGT CTCATGGGGT GGGCCATGAT AGGTACCTAG TGGTAGGTAG AAAAAGACGG 
AAGGAAGACC TACTAGATCA GCTCAAACAG GAAGGCCAAG TGGGCCATTA CGAGCTTGTT 
ATGCCTCAAG CAAATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

NKRF ( NM_017544.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060014.3 Homo sapiens NFKB repressing factor (NKRF), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu109248
Clone ID Related Accession (Same CDS sequence)	NM_017544.5
Accession Version	NM_017544.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2355bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-19
Organism	Homo sapiens(human)
Product	NF-kappa-B-repressing factor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from LPVO02000272.1, AY208891.1 and AA845622.1. On Jul 19, 2019 this sequence version replaced NM_017544.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR7346977.1571299.1, SRR6380202.143827.7 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTGGAG GACGTCTGCT GTTGGGGGGC GACTTCCTGT CGCCGCCGCC GCTGCCCCCC 
CTCCCGCCGC CGCCGCTGCC GCCCCTCCCG CCGCCCCCGC CCGAGCCAGT GCTGGAGCAG 
TGGCGCTATA GCCACGAAAG TGACTGGCAG TGGGCTCTGC GGCGCAGCTT CATCTGTCGG 
CACCTGCACA GCTATCCCGG GGCTGCCCTC GACCAGCTCC TCGCGCTCTC CGCCGCCTGG 
ACCAACCACG TCTTCCTGGG CTGCAGGTAC AGCCCACGCT TGATGGAAAA AATTCTCCAA 
ATGGCTGAAG GTATTGATAT TGGGGAGATG CCTTCATATG ATCTGGTGCT GTCCAAACCT 
TCCAAAGGTC AAAAACGCCA CCTCTCAACA TGTGATGGTC AAAATCCTCC TAAAAAGCAA 
GCCGGTTCCA AATTCCATGC GAGACCTCGT TTTGAGCCTG TACATTTTGT AGCTAGTAGT 
TCAAAAGATG AAAGACAGGA AGATCCTTAT GGCCCTCAAA CAAAAGAGGT AAATGAACAA 
ACACATTTTG CCAGCATGCC AAGAGACATC TACCAAGATT ATACTCAAGA CTCTTTCAGT 
ATACAAGATG GGAATTCTCA GTATTGTGAT TCATCAGGAT TCATTCTCAC AAAAGACCAG 
CCTGTAACAG CCAACATGTA TTTTGACAGT GGGAACCCTG CCCCAAGCAC CACATCACAG 
CAGGCAAACT CTCAGTCAAC TCCTGAGCCT TCACCATCAC AGACATTTCC CGAGTCTGTG 
GTAGCCGAGA AGCAGTATTT TATTGAAAAA TTAACGGCGA CAATCTGGAA GAACCTTTCT 
AATCCAGAAA TGACTTCTGG ATCTGATAAA ATTAATTATA CATATATGTT AACTCGTTGT 
ATTCAGGCGT GTAAGACAAA TCCTGAGTAT ATATATGCTC CTTTAAAGGA AATTCCTCCT 
GCCGACATCC CCAAAAATAA AAAACTTCTA ACTGATGGCT ATGCTTGTGA AGTTAGATGC 
CAAAATATCT ACTTAACTAC AGGTTATGCT GGCAGCAAGA ATGGGTCCAG GGATCGAGCT 
ACAGAGCTAG CTGTAAAACT CTTGCAGAAA CGTATTGAAG TTAGAGTTGT CCGGCGGAAA 
TTCAAGCATA CATTTGGAGA GGACCTCGTG GTGTGTCAGA TTGGCATGTC CTCCTATGAA 
TTTCCTCCAG CTCTGAAGCC ACCAGAAGAC CTGGTGGTGC TGGGTAAAGA TGCTTCCGGG 
CAGCCAATTT TTAATGCTTC TGCCAAACAC TGGACCAATT TTGTCATTAC AGAAAATGCA 
AATGATGCAA TTGGTATCCT TAACAATTCT GCCTCATTCA ACAAGATGTC AATTGAATAC 
AAATATGAGA TGATGCCAAA TCGCACATGG CGTTGTCGAG TGTTTTTACA AGATCACTGC 
TTAGCTGAAG GTTATGGAAC CAAGAAAACA AGTAAACATG CAGCTGCCGA CGAGGCTTTG 
AAAATTCTTC AAAAAACACA GCCCACTTAT CCATCTGTCA AAAGTTCACA ATGCCATACA 
GGCTCTTCAC CCAGAGGATC TGGAAAGAAG AAAGATATAA AGGATCTTGT AGTTTATGAG 
AATTCTTCAA ATCCCGTGTG CACGCTGAAC GACACAGCTC AGTTTAACCG AATGACAGTT 
GAGTATGTCT ATGAAAGGAT GACAGGCCTC CGCTGGAAAT GCAAAGTGAT TCTAGAGAGT 
GAAGTAATTG CAGAAGCAGT TGGGGTGAAG AAAACTGTCA AATATGAAGC TGCTGGGGAA 
GCTGTGAAAA CCCTCAAAAA GACCCAGCCA ACTGTCATTA ACAACTTGAA GAAAGGAGCT 
GTTGAAGATG TGATTTCAAG AAATGAAATT CAGGGCCGCT CAGCAGAGGA GGCTTACAAA 
CAGCAAATCA AAGAAGATAA TATTGGAAAT CAGCTGCTGA GAAAGATGGG TTGGACTGGT 
GGTGGTTTAG GTAAATCTGG TGAGGGCATA CGGGAGCCTA TCTCAGTGAA AGAGCAGCAT 
AAGCGGGAAG GGCTTGGTCT GGATGTAGAG AGGGTGAATA AAATTGCCAA GAGAGATATT 
GAACAGATCA TCAGAAACTA CGCCCGCTCC GAGAGCCACA CAGATTTGAC TTTCTCTAGA 
GAGCTGACTA ATGATGAACG GAAGCAAATA CATCAGATTG CCCAGAAGTA TGGTCTTAAG 
AGTAAGTCTC ATGGGGTGGG CCATGATAGG TACCTAGTGG TAGGTAGAAA AAGACGGAAG 
GAAGACCTAC TAGATCAGCT CAAACAGGAA GGCCAAGTGG GCCATTACGA GCTTGTTATG 
CCTCAAGCAA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060014.3
CDS	430..2784
Translation	MAGGRLLLGGDFLSPPPLPPLPPPPLPPLPPPPPEPVLEQWRYSHESDWQWALRRSFICRHLHSYPGAALDQLLALSAAWTNHVFLGCRYSPRLMEKILQMAEGIDIGEMPSYDLVLSKPSKGQKRHLSTCDGQNPPKKQAGSKFHARPRFEPVHFVASSSKDERQEDPYGPQTKEVNEQTHFASMPRDIYQDYTQDSFSIQDGNSQYCDSSGFILTKDQPVTANMYFDSGNPAPSTTSQQANSQSTPEPSPSQTFPESVVAEKQYFIEKLTATIWKNLSNPEMTSGSDKINYTYMLTRCIQACKTNPEYIYAPLKEIPPADIPKNKKLLTDGYACEVRCQNIYLTTGYAGSKNGSRDRATELAVKLLQKRIEVRVVRRKFKHTFGEDLVVCQIGMSSYEFPPALKPPEDLVVLGKDASGQPIFNASAKHWTNFVITENANDAIGILNNSASFNKMSIEYKYEMMPNRTWRCRVFLQDHCLAEGYGTKKTSKHAAADEALKILQKTQPTYPSVKSSQCHTGSSPRGSGKKKDIKDLVVYENSSNPVCTLNDTAQFNRMTVEYVYERMTGLRWKCKVILESEVIAEAVGVKKTVKYEAAGEAVKTLKKTQPTVINNLKKGAVEDVISRNEIQGRSAEEAYKQQIKEDNIGNQLLRKMGWTGGGLGKSGEGIREPISVKEQHKREGLGLDVERVNKIAKRDIEQIIRNYARSESHTDLTFSRELTNDERKQIHQIAQKYGLKSKSHGVGHDRYLVVGRKRRKEDLLDQLKQEGQVGHYELVMPQAN

Target ORF information:

RefSeq Version	NM_017544.5
Organism	Homo sapiens(human)
Definition	Homo sapiens NFKB repressing factor (NKRF), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_017544.5

ORF Insert Sequence:

ATGGCTGGAG GACGTCTGCT GTTGGGGGGC GACTTCCTGT CGCCGCCGCC GCTGCCCCCC 
CTCCCGCCGC CGCCGCTGCC GCCCCTCCCG CCGCCCCCGC CCGAGCCAGT GCTGGAGCAG 
TGGCGCTATA GCCACGAAAG TGACTGGCAG TGGGCTCTGC GGCGCAGCTT CATCTGTCGG 
CACCTGCACA GCTATCCCGG GGCTGCCCTC GACCAGCTCC TCGCGCTCTC CGCCGCCTGG 
ACCAACCACG TCTTCCTGGG CTGCAGGTAC AGCCCACGCT TGATGGAAAA AATTCTCCAA 
ATGGCTGAAG GTATTGATAT TGGGGAGATG CCTTCATATG ATCTGGTGCT GTCCAAACCT 
TCCAAAGGTC AAAAACGCCA CCTCTCAACA TGTGATGGTC AAAATCCTCC TAAAAAGCAA 
GCCGGTTCCA AATTCCATGC GAGACCTCGT TTTGAGCCTG TACATTTTGT AGCTAGTAGT 
TCAAAAGATG AAAGACAGGA AGATCCTTAT GGCCCTCAAA CAAAAGAGGT AAATGAACAA 
ACACATTTTG CCAGCATGCC AAGAGACATC TACCAAGATT ATACTCAAGA CTCTTTCAGT 
ATACAAGATG GGAATTCTCA GTATTGTGAT TCATCAGGAT TCATTCTCAC AAAAGACCAG 
CCTGTAACAG CCAACATGTA TTTTGACAGT GGGAACCCTG CCCCAAGCAC CACATCACAG 
CAGGCAAACT CTCAGTCAAC TCCTGAGCCT TCACCATCAC AGACATTTCC CGAGTCTGTG 
GTAGCCGAGA AGCAGTATTT TATTGAAAAA TTAACGGCGA CAATCTGGAA GAACCTTTCT 
AATCCAGAAA TGACTTCTGG ATCTGATAAA ATTAATTATA CATATATGTT AACTCGTTGT 
ATTCAGGCGT GTAAGACAAA TCCTGAGTAT ATATATGCTC CTTTAAAGGA AATTCCTCCT 
GCCGACATCC CCAAAAATAA AAAACTTCTA ACTGATGGCT ATGCTTGTGA AGTTAGATGC 
CAAAATATCT ACTTAACTAC AGGTTATGCT GGCAGCAAGA ATGGGTCCAG GGATCGAGCT 
ACAGAGCTAG CTGTAAAACT CTTGCAGAAA CGTATTGAAG TTAGAGTTGT CCGGCGGAAA 
TTCAAGCATA CATTTGGAGA GGACCTCGTG GTGTGTCAGA TTGGCATGTC CTCCTATGAA 
TTTCCTCCAG CTCTGAAGCC ACCAGAAGAC CTGGTGGTGC TGGGTAAAGA TGCTTCCGGG 
CAGCCAATTT TTAATGCTTC TGCCAAACAC TGGACCAATT TTGTCATTAC AGAAAATGCA 
AATGATGCAA TTGGTATCCT TAACAATTCT GCCTCATTCA ACAAGATGTC AATTGAATAC 
AAATATGAGA TGATGCCAAA TCGCACATGG CGTTGTCGAG TGTTTTTACA AGATCACTGC 
TTAGCTGAAG GTTATGGAAC CAAGAAAACA AGTAAACATG CAGCTGCCGA CGAGGCTTTG 
AAAATTCTTC AAAAAACACA GCCCACTTAT CCATCTGTCA AAAGTTCACA ATGCCATACA 
GGCTCTTCAC CCAGAGGATC TGGAAAGAAG AAAGATATAA AGGATCTTGT AGTTTATGAG 
AATTCTTCAA ATCCCGTGTG CACGCTGAAC GACACAGCTC AGTTTAACCG AATGACAGTT 
GAGTATGTCT ATGAAAGGAT GACAGGCCTC CGCTGGAAAT GCAAAGTGAT TCTAGAGAGT 
GAAGTAATTG CAGAAGCAGT TGGGGTGAAG AAAACTGTCA AATATGAAGC TGCTGGGGAA 
GCTGTGAAAA CCCTCAAAAA GACCCAGCCA ACTGTCATTA ACAACTTGAA GAAAGGAGCT 
GTTGAAGATG TGATTTCAAG AAATGAAATT CAGGGCCGCT CAGCAGAGGA GGCTTACAAA 
CAGCAAATCA AAGAAGATAA TATTGGAAAT CAGCTGCTGA GAAAGATGGG TTGGACTGGT 
GGTGGTTTAG GTAAATCTGG TGAGGGCATA CGGGAGCCTA TCTCAGTGAA AGAGCAGCAT 
AAGCGGGAAG GGCTTGGTCT GGATGTAGAG AGGGTGAATA AAATTGCCAA GAGAGATATT 
GAACAGATCA TCAGAAACTA CGCCCGCTCC GAGAGCCACA CAGATTTGAC TTTCTCTAGA 
GAGCTGACTA ATGATGAACG GAAGCAAATA CATCAGATTG CCCAGAAGTA TGGTCTTAAG 
AGTAAGTCTC ATGGGGTGGG CCATGATAGG TACCTAGTGG TAGGTAGAAA AAGACGGAAG 
GAAGACCTAC TAGATCAGCT CAAACAGGAA GGCCAAGTGG GCCATTACGA GCTTGTTATG 
CCTCAAGCAA ATTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Characterization of host proteins interacting with the lymphocytic choriomeningitis virus L protein.
PLoS pathogens13(12)e1006758(2017 Dec)
Khamina K,Lercher A,Caldera M,Schliehe C,Vilagos B,Sahin M,Kosack L,Bhattacharya A,M?jek P,Stukalov A,Sacco R,James LC,Pinschewer DD,Bennett KL,Menche J,Bergthaler A

Protein interaction network of alternatively spliced NudCD1 isoforms.
Scientific reports7(1)12987(2017 Oct)
Asselin-Mullen P,Chauvin A,Dubois ML,Drissi R,L?vesque D,Boisvert FM

The G-patch protein NF-?B-repressing factor mediates the recruitment of the exonuclease XRN2 and activation of the RNA helicase DHX15 in human ribosome biogenesis.
Nucleic acids research45(9)5359-5374(2017 May)
Memet I,Doebele C,Sloan KE,Bohnsack MT

Human NF-?B repressing factor acts as a stress-regulated switch for ribosomal RNA processing and nucleolar homeostasis surveillance.
Proceedings of the National Academy of Sciences of the United States of America114(5)1045-1050(2017 Jan)
Coccia M,Rossi A,Riccio A,Trotta E,Santoro MG

NF-?B-repressing factor phosphorylation regulates transcription elongation via its interactions with 5'?3' exoribonuclease 2 and negative elongation factor.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology30(1)174-85(2016 Jan)
Rother S,Bartels M,Schweda AT,Resch K,Pallua N,Nourbakhsh M

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NKRF cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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