PKN1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	PKN1
Entrez Gene ID	5585
Full Name	protein kinase N1
Synonyms	DBK,PAK-1,PAK1,PKN,PKN-ALPHA,PRK1,PRKCL1
General protein information	Preferred Names protein kinase N1 Names serine/threonine-protein kinase N1 protease-activated kinase 1 protein kinase C-like 1 protein kinase C-like PKN protein kinase C-related kinase 1 protein kinase PKN-alpha serine-threonine kinase N serine/threonine protein kinase N
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 19 Map Location: 19p13.12
Summary	The protein encoded by this gene belongs to the protein kinase C superfamily. This kinase is activated by Rho family of small G proteins and may mediate the Rho-dependent signaling pathway. This kinase can be activated by phospholipids and by limited proteolysis. The 3-phosphoinositide dependent protein kinase-1 (PDPK1/PDK1) is reported to phosphorylate this kinase, which may mediate insulin signals to the actin cytoskeleton. The proteolytic activation of this kinase by caspase-3 or related proteases during apoptosis suggests its role in signal transduction related to apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008].
Disorder MIM:	601032

mRNA and Protein(s)

mRNA	Protein	Name
NM_213560.1	NP_998725.1	serine/threonine-protein kinase N1 isoform 1
NM_213560.2	NP_998725.1	serine/threonine-protein kinase N1 isoform 1
NM_213560.3	NP_998725.1	serine/threonine-protein kinase N1 isoform 1
NM_002741.5	NP_002732.3	serine/threonine-protein kinase N1 isoform 2
NM_002741.3	NP_002732.3	serine/threonine-protein kinase N1 isoform 2
NM_002741.4	NP_002732.3	serine/threonine-protein kinase N1 isoform 2
XM_011528128.1	XP_011526430.1	serine/threonine-protein kinase N1 isoform X1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	PKN1	XP_512443.3
Danio rerio (zebrafish)	LOC560840	XP_005160108.1
Homo sapiens (human)	PKN1	NP_002732.3
Canis lupus familiaris (dog)	PKN1	XP_003639827.1
Bos taurus (cattle)	PKN1	NP_001073715.1
Rattus norvegicus (Norway rat)	Pkn1	NP_058871.2
Danio rerio (zebrafish)	zgc:162290	NP_001082929.1
	LOC718834	XP_002808221.1
Mus musculus (house mouse)	Pkn1	NP_796236.2

Gene Ontology
Bibliography

Related articles in PubMed

The protein kinase C super-family member PKN is regulated by mTOR and influences differentiation during prostate cancer progression.
Yang CS, Melhuish TA, Spencer A, Ni L, Hao Y, Jividen K, Harris TE, Snow C, Frierson HF Jr, Wotton D, Paschal BM
The Prostate77(15)1452-1467(2017 Nov)

RhoA promotes epidermal stem cell proliferation via PKN1-cyclin D1 signaling.
Wang F, Zhan R, Chen L, Dai X, Wang W, Guo R, Li X, Li Z, Wang L, Huang S, Shen J, Li S, Cao C
PloS one12(2)e0172613(2017)

Protein kinase C-related kinase 1 and 2 play an essential role in thromboxane-mediated neoplastic responses in prostate cancer.
O'Sullivan AG, Mulvaney EP, Hyland PB, Kinsella BT
Oncotarget6(28)26437-56(2015 Sep)

PRK1/PKN1 controls migration and metastasis of androgen-independent prostate cancer cells.
Jilg CA, Ketscher A, Metzger E, Hummel B, Willmann D, R?sseler V, Drendel V, Imhof A, Jung M, Franz H, H?lz S, Kr?nig M, M?ller JM, Sch?le R
Oncotarget5(24)12646-64(2014 Dec)

Enzyme kinetics and distinct modulation of the protein kinase N family of kinases by lipid activators and small molecule inhibitors.
Falk MD, Liu W, Bola?os B, Unsal-Kacmaz K, Klippel A, Grant S, Brooun A, Timofeevski S
Bioscience reports34(2)(2014 Apr)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Pkn1 is not required for tumorigenesis initiated by loss of Pten. Triple knockout of Pten, Pkn1, and Pkn2 in mouse prostate results in squamous cell carcinoma, an uncommon but therapy-resistant form of prostate cancer.
Title: The protein kinase C super-family member PKN is regulated by mTOR and influences differentiation during prostate cancer progression.

PKN1 activity was up-regulated by the active RhoA mutant (G14V) and suppressed by RhoA T19N. PKN1 siRNA interrupted the ability of RhoA to promote ESC proliferation and DNA synthesis. The effect of RhoA on ESC proliferation is mediated by activation of the PKN1-cyclin D1 pathway in vitro.
Title: RhoA promotes epidermal stem cell proliferation via PKN1-cyclin D1 signaling.

TXA2-mediated neoplastic responses in prostate adenocarcinoma PC-3 cells occur through a PRK1/PRK2-dependent mechanism.
Title: Protein kinase C-related kinase 1 and 2 play an essential role in thromboxane-mediated neoplastic responses in prostate cancer.

Transcriptome and interactome analyses uncover that PRK1 regulates expression of migration-relevant genes by interacting with the scaffold protein sperm-associated antigen 9 (SPAG9/JIP4).
Title: PRK1/PKN1 controls migration and metastasis of androgen-independent prostate cancer cells.

Steady-state kinetic analysis revealed that PKN1-3 follows a sequential ordered Bi-Bi kinetic mechanism, where peptide substrate binding is preceded by ATP binding. This kinetic mechanism was confirmed by additional kinetic studies for product inhibition and affinity of small molecule inhibitors.
Title: Enzyme kinetics and distinct modulation of the protein kinase N family of kinases by lipid activators and small molecule inhibitors.

The following PKN1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the PKN1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu17093	NM_002741.3 Latest version!	Homo sapiens protein kinase N1 (PKN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu13518	NM_213560.1 Latest version!	Homo sapiens protein kinase N1 (PKN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$657.30 $939.00
OHu59922	XM_011528128.1 Latest version!	Homo sapiens protein kinase N1 (PKN1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OHu107138	NM_213560.3 Latest version!	Homo sapiens protein kinase N1 (PKN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu107140	NM_002741.5 Latest version!	Homo sapiens protein kinase N1 (PKN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
View Old Accession Versions >>
OHu107138	NM_213560.2	Homo sapiens protein kinase N1 (PKN1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OHu107140	NM_002741.4	Homo sapiens protein kinase N1 (PKN1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

PKN1 ( NM_002741.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_002732.3 Homo sapiens protein kinase N1 (PKN1), transcript variant 2, mRNA.

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CloneID	OHu17093
Clone ID Related Accession (Same CDS sequence)	NM_002741.3
Accession Version	NM_002741.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase N1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BQ946818.1, BC040061.1, D26181.1, U33053.1 and BU628889.1. On May 12, 2004 this sequence version replaced NM_002741.2. Transcript Variant: This variant (2) uses an alternate exon at the 5' end compared to variant 1, which includes a part of the coding region. The resulting isoform (2) has a distinct and shorter N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040061.1, S75546.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGCG ACGCCGTGCA GAGTGAGCCT CGCAGCTGGT CCCTGCTAGA GCAGCTGGGC 
CTGGCCGGGG CAGACCTGGC GGCCCCCGGG GTACAGCAGC AGCTGGAGCT GGAGCGGGAG 
CGGCTGCGGC GGGAAATCCG CAAGGAGCTG AAGCTGAAGG AGGGTGCTGA GAACCTGCGG 
CGGGCCACCA CTGACCTGGG CCGCAGCCTG GGCCCCGTAG AGCTGCTGCT GCGGGGCTCC 
TCGCGCCGCC TCGACCTGCT GCACCAGCAG CTGCAGGAGC TGCACGCCCA CGTGGTGCTT 
CCCGACCCGG CGGCCACCCA CGATGGCCCC CAGTCCCCTG GTGCGGGTGG CCCCACCTGC 
TCGGCCACCA ACCTGAGCCG CGTGGCGGGC CTGGAGAAGC AGTTGGCCAT TGAGCTGAAG 
GTGAAGCAGG GGGCGGAGAA CATGATCCAG ACCTACAGCA ATGGCAGCAC CAAGGACCGG 
AAGCTGCTGC TGACAGCCCA GCAGATGTTG CAGGACAGTA AGACCAAGAT TGACATCATC 
CGCATGCAAC TCCGCCGGGC GCTGCAGGCC GGCCAGCTGG AGAACCAGGC AGCCCCGGAT 
GACACCCAAG GGAGTCCTGA CCTGGGGGCT GTGGAGCTGC GCATCGAAGA GCTGCGGCAC 
CACTTCCGAG TGGAGCACGC GGTGGCCGAG GGTGCCAAGA ACGTACTGCG CCTGCTCAGC 
GCTGCCAAGG CCCCGGACCG CAAGGCAGTC AGCGAGGCCC AGGAGAAATT GACAGAATCC 
AACCAGAAGC TGGGGCTGCT GCGGGAGGCT CTGGAGCGGA GACTTGGGGA GCTGCCCGCC 
GACCACCCCA AGGGGCGGCT GCTGCGAGAA GAGCTCGCTG CGGCCTCCTC CGCTGCCTTC 
AGCACCCGCC TGGCCGGGCC CTTTCCCGCC ACGCACTACA GCACCCTGTG CAAGCCCGCG 
CCGCTCACAG GGACCCTGGA GGTACGAGTG GTGGGCTGCA GAGACCTCCC AGAGACCATC 
CCGTGGAACC CTACCCCCTC AATGGGGGGA CCTGGGACCC CAGACAGCCG CCCCCCCTTC 
CTGAGCCGCC CAGCCCGGGG CCTTTACAGC CGAAGCGGAA GCCTCAGTGG CCGGAGCAGC 
CTCAAAGCAG AAGCCGAGAA CACCAGTGAA GTCAGCACTG TGCTTAAGCT GGATAACACA 
GTGGTGGGGC AGACGTCTTG GAAGCCATGT GGCCCCAATG CCTGGGACCA GAGCTTCACT 
CTGGAGCTGG AAAGGGCACG GGAACTGGAG TTGGCTGTGT TCTGGCGGGA CCAGCGGGGC 
CTGTGTGCCC TCAAATTCCT GAAGTTGGAG GATTTCTTGG ACAATGAGAG GCATGAGGTG 
CAGCTGGACA TGGAACCCCA GGGCTGCCTG GTGGCTGAGG TCACCTTCCG CAACCCTGTC 
ATTGAGAGGA TTCCTCGGCT CCGACGGCAG AAGAAAATTT TCTCCAAGCA GCAAGGGAAG 
GCGTTCCAGC GTGCTAGGCA GATGAACATC GATGTCGCCA CGTGGGTGCG GCTGCTCCGG 
AGGCTCATCC CCAATGCCAC GGGCACAGGC ACCTTTAGCC CTGGGGCTTC TCCAGGATCC 
GAGGCCCGGA CCACGGGTGA CATATCGGTG GAGAAGCTGA ACCTCGGCAC TGACTCGGAC 
AGCTCACCTC AGAAGAGCTC GCGGGATCCT CCTTCCAGCC CATCGAGCCT GAGCTCCCCC 
ATCCAGGAAT CCACTGCTCC CGAGCTGCCT TCGGAGACCC AGGAGACCCC AGGCCCCGCC 
CTGTGCAGCC CTCTGAGGAA GTCACCTCTG ACCCTCGAAG ATTTCAAGTT CCTGGCGGTG 
CTGGGCCGGG GTCATTTTGG GAAGGTGCTC CTCTCCGAAT TCCGGCCCAG TGGGGAGCTG 
TTCGCCATCA AGGCTCTGAA GAAAGGGGAC ATTGTGGCCC GAGACGAGGT GGAGAGCCTG 
ATGTGTGAGA AGCGGATATT GGCGGCAGTG ACCAGTGCGG GACACCCCTT CCTGGTGAAC 
CTCTTCGGCT GTTTCCAGAC ACCGGAGCAC GTGTGCTTCG TGATGGAGTA CTCGGCCGGT 
GGGGACCTGA TGCTGCACAT CCACAGCGAC GTGTTCTCTG AGCCCCGTGC CATCTTTTAT 
TCCGCCTGCG TGGTGCTGGG CCTACAGTTT CTTCACGAAC ACAAGATCGT CTACAGGGAC 
CTGAAGTTGG ACAATTTGCT CCTGGACACC GAGGGCTACG TCAAGATCGC AGACTTTGGC 
CTCTGCAAGG AGGGGATGGG CTATGGGGAC CGGACCAGCA CATTCTGTGG GACCCCGGAG 
TTCCTGGCCC CTGAGGTGCT GACGGACACG TCGTACACGC GAGCTGTGGA CTGGTGGGGA 
CTGGGTGTGC TGCTCTACGA GATGCTGGTT GGCGAGTCCC CATTCCCAGG GGATGATGAG 
GAGGAGGTCT TCGACAGCAT CGTCAACGAC GAGGTTCGCT ACCCCCGCTT CCTGTCGGCC 
GAAGCCATCG GCATCATGAG AAGGCTGCTT CGGAGGAACC CAGAGCGGAG GCTGGGATCT 
AGCGAGAGAG ATGCAGAAGA TGTGAAGAAA CAGCCCTTCT TCAGGACTCT GGGCTGGGAA 
GCCCTGTTGG CCCGGCGCCT GCCACCGCCC TTTGTGCCCA CGCTGTCCGG CCGCACCGAC 
GTCAGCAACT TCGACGAGGA GTTCACCGGG GAGGCCCCCA CACTGAGCCC GCCCCGCGAC 
GCGCGGCCCC TCACAGCCGC GGAGCAGGCA GCCTTCCTGG ACTTCGACTT CGTGGCCGGG 
GGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002732.3
CDS	169..2997
Translation	MASDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC

Target ORF information:

RefSeq Version	NM_002741.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase N1 (PKN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002741.3

ORF Insert Sequence:

ATGGCCAGCG ACGCCGTGCA GAGTGAGCCT CGCAGCTGGT CCCTGCTAGA GCAGCTGGGC 
CTGGCCGGGG CAGACCTGGC GGCCCCCGGG GTACAGCAGC AGCTGGAGCT GGAGCGGGAG 
CGGCTGCGGC GGGAAATCCG CAAGGAGCTG AAGCTGAAGG AGGGTGCTGA GAACCTGCGG 
CGGGCCACCA CTGACCTGGG CCGCAGCCTG GGCCCCGTAG AGCTGCTGCT GCGGGGCTCC 
TCGCGCCGCC TCGACCTGCT GCACCAGCAG CTGCAGGAGC TGCACGCCCA CGTGGTGCTT 
CCCGACCCGG CGGCCACCCA CGATGGCCCC CAGTCCCCTG GTGCGGGTGG CCCCACCTGC 
TCGGCCACCA ACCTGAGCCG CGTGGCGGGC CTGGAGAAGC AGTTGGCCAT TGAGCTGAAG 
GTGAAGCAGG GGGCGGAGAA CATGATCCAG ACCTACAGCA ATGGCAGCAC CAAGGACCGG 
AAGCTGCTGC TGACAGCCCA GCAGATGTTG CAGGACAGTA AGACCAAGAT TGACATCATC 
CGCATGCAAC TCCGCCGGGC GCTGCAGGCC GGCCAGCTGG AGAACCAGGC AGCCCCGGAT 
GACACCCAAG GGAGTCCTGA CCTGGGGGCT GTGGAGCTGC GCATCGAAGA GCTGCGGCAC 
CACTTCCGAG TGGAGCACGC GGTGGCCGAG GGTGCCAAGA ACGTACTGCG CCTGCTCAGC 
GCTGCCAAGG CCCCGGACCG CAAGGCAGTC AGCGAGGCCC AGGAGAAATT GACAGAATCC 
AACCAGAAGC TGGGGCTGCT GCGGGAGGCT CTGGAGCGGA GACTTGGGGA GCTGCCCGCC 
GACCACCCCA AGGGGCGGCT GCTGCGAGAA GAGCTCGCTG CGGCCTCCTC CGCTGCCTTC 
AGCACCCGCC TGGCCGGGCC CTTTCCCGCC ACGCACTACA GCACCCTGTG CAAGCCCGCG 
CCGCTCACAG GGACCCTGGA GGTACGAGTG GTGGGCTGCA GAGACCTCCC AGAGACCATC 
CCGTGGAACC CTACCCCCTC AATGGGGGGA CCTGGGACCC CAGACAGCCG CCCCCCCTTC 
CTGAGCCGCC CAGCCCGGGG CCTTTACAGC CGAAGCGGAA GCCTCAGTGG CCGGAGCAGC 
CTCAAAGCAG AAGCCGAGAA CACCAGTGAA GTCAGCACTG TGCTTAAGCT GGATAACACA 
GTGGTGGGGC AGACGTCTTG GAAGCCATGT GGCCCCAATG CCTGGGACCA GAGCTTCACT 
CTGGAGCTGG AAAGGGCACG GGAACTGGAG TTGGCTGTGT TCTGGCGGGA CCAGCGGGGC 
CTGTGTGCCC TCAAATTCCT GAAGTTGGAG GATTTCTTGG ACAATGAGAG GCATGAGGTG 
CAGCTGGACA TGGAACCCCA GGGCTGCCTG GTGGCTGAGG TCACCTTCCG CAACCCTGTC 
ATTGAGAGGA TTCCTCGGCT CCGACGGCAG AAGAAAATTT TCTCCAAGCA GCAAGGGAAG 
GCGTTCCAGC GTGCTAGGCA GATGAACATC GATGTCGCCA CGTGGGTGCG GCTGCTCCGG 
AGGCTCATCC CCAATGCCAC GGGCACAGGC ACCTTTAGCC CTGGGGCTTC TCCAGGATCC 
GAGGCCCGGA CCACGGGTGA CATATCGGTG GAGAAGCTGA ACCTCGGCAC TGACTCGGAC 
AGCTCACCTC AGAAGAGCTC GCGGGATCCT CCTTCCAGCC CATCGAGCCT GAGCTCCCCC 
ATCCAGGAAT CCACTGCTCC CGAGCTGCCT TCGGAGACCC AGGAGACCCC AGGCCCCGCC 
CTGTGCAGCC CTCTGAGGAA GTCACCTCTG ACCCTCGAAG ATTTCAAGTT CCTGGCGGTG 
CTGGGCCGGG GTCATTTTGG GAAGGTGCTC CTCTCCGAAT TCCGGCCCAG TGGGGAGCTG 
TTCGCCATCA AGGCTCTGAA GAAAGGGGAC ATTGTGGCCC GAGACGAGGT GGAGAGCCTG 
ATGTGTGAGA AGCGGATATT GGCGGCAGTG ACCAGTGCGG GACACCCCTT CCTGGTGAAC 
CTCTTCGGCT GTTTCCAGAC ACCGGAGCAC GTGTGCTTCG TGATGGAGTA CTCGGCCGGT 
GGGGACCTGA TGCTGCACAT CCACAGCGAC GTGTTCTCTG AGCCCCGTGC CATCTTTTAT 
TCCGCCTGCG TGGTGCTGGG CCTACAGTTT CTTCACGAAC ACAAGATCGT CTACAGGGAC 
CTGAAGTTGG ACAATTTGCT CCTGGACACC GAGGGCTACG TCAAGATCGC AGACTTTGGC 
CTCTGCAAGG AGGGGATGGG CTATGGGGAC CGGACCAGCA CATTCTGTGG GACCCCGGAG 
TTCCTGGCCC CTGAGGTGCT GACGGACACG TCGTACACGC GAGCTGTGGA CTGGTGGGGA 
CTGGGTGTGC TGCTCTACGA GATGCTGGTT GGCGAGTCCC CATTCCCAGG GGATGATGAG 
GAGGAGGTCT TCGACAGCAT CGTCAACGAC GAGGTTCGCT ACCCCCGCTT CCTGTCGGCC 
GAAGCCATCG GCATCATGAG AAGGCTGCTT CGGAGGAACC CAGAGCGGAG GCTGGGATCT 
AGCGAGAGAG ATGCAGAAGA TGTGAAGAAA CAGCCCTTCT TCAGGACTCT GGGCTGGGAA 
GCCCTGTTGG CCCGGCGCCT GCCACCGCCC TTTGTGCCCA CGCTGTCCGG CCGCACCGAC 
GTCAGCAACT TCGACGAGGA GTTCACCGGG GAGGCCCCCA CACTGAGCCC GCCCCGCGAC 
GCGCGGCCCC TCACAGCCGC GGAGCAGGCA GCCTTCCTGG ACTTCGACTT CGTGGCCGGG 
GGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PKN1 ( NM_213560.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_998725.1 Homo sapiens protein kinase N1 (PKN1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu13518
Clone ID Related Accession (Same CDS sequence)	NM_213560.1
Accession Version	NM_213560.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase N1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK123007.1, U33053.1 and BU628889.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK123007.1, AK292130.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CCAATAACCC CTCGGAGCAG GAGCTGGAGA GTGAGCCTCG CAGCTGGTCC 
CTGCTAGAGC AGCTGGGCCT GGCCGGGGCA GACCTGGCGG CCCCCGGGGT ACAGCAGCAG 
CTGGAGCTGG AGCGGGAGCG GCTGCGGCGG GAAATCCGCA AGGAGCTGAA GCTGAAGGAG 
GGTGCTGAGA ACCTGCGGCG GGCCACCACT GACCTGGGCC GCAGCCTGGG CCCCGTAGAG 
CTGCTGCTGC GGGGCTCCTC GCGCCGCCTC GACCTGCTGC ACCAGCAGCT GCAGGAGCTG 
CACGCCCACG TGGTGCTTCC CGACCCGGCG GCCACCCACG ATGGCCCCCA GTCCCCTGGT 
GCGGGTGGCC CCACCTGCTC GGCCACCAAC CTGAGCCGCG TGGCGGGCCT GGAGAAGCAG 
TTGGCCATTG AGCTGAAGGT GAAGCAGGGG GCGGAGAACA TGATCCAGAC CTACAGCAAT 
GGCAGCACCA AGGACCGGAA GCTGCTGCTG ACAGCCCAGC AGATGTTGCA GGACAGTAAG 
ACCAAGATTG ACATCATCCG CATGCAACTC CGCCGGGCGC TGCAGGCCGG CCAGCTGGAG 
AACCAGGCAG CCCCGGATGA CACCCAAGGG AGTCCTGACC TGGGGGCTGT GGAGCTGCGC 
ATCGAAGAGC TGCGGCACCA CTTCCGAGTG GAGCACGCGG TGGCCGAGGG TGCCAAGAAC 
GTACTGCGCC TGCTCAGCGC TGCCAAGGCC CCGGACCGCA AGGCAGTCAG CGAGGCCCAG 
GAGAAATTGA CAGAATCCAA CCAGAAGCTG GGGCTGCTGC GGGAGGCTCT GGAGCGGAGA 
CTTGGGGAGC TGCCCGCCGA CCACCCCAAG GGGCGGCTGC TGCGAGAAGA GCTCGCTGCG 
GCCTCCTCCG CTGCCTTCAG CACCCGCCTG GCCGGGCCCT TTCCCGCCAC GCACTACAGC 
ACCCTGTGCA AGCCCGCGCC GCTCACAGGG ACCCTGGAGG TACGAGTGGT GGGCTGCAGA 
GACCTCCCAG AGACCATCCC GTGGAACCCT ACCCCCTCAA TGGGGGGACC TGGGACCCCA 
GACAGCCGCC CCCCCTTCCT GAGCCGCCCA GCCCGGGGCC TTTACAGCCG AAGCGGAAGC 
CTCAGTGGCC GGAGCAGCCT CAAAGCAGAA GCCGAGAACA CCAGTGAAGT CAGCACTGTG 
CTTAAGCTGG ATAACACAGT GGTGGGGCAG ACGTCTTGGA AGCCATGTGG CCCCAATGCC 
TGGGACCAGA GCTTCACTCT GGAGCTGGAA AGGGCACGGG AACTGGAGTT GGCTGTGTTC 
TGGCGGGACC AGCGGGGCCT GTGTGCCCTC AAATTCCTGA AGTTGGAGGA TTTCTTGGAC 
AATGAGAGGC ATGAGGTGCA GCTGGACATG GAACCCCAGG GCTGCCTGGT GGCTGAGGTC 
ACCTTCCGCA ACCCTGTCAT TGAGAGGATT CCTCGGCTCC GACGGCAGAA GAAAATTTTC 
TCCAAGCAGC AAGGGAAGGC GTTCCAGCGT GCTAGGCAGA TGAACATCGA TGTCGCCACG 
TGGGTGCGGC TGCTCCGGAG GCTCATCCCC AATGCCACGG GCACAGGCAC CTTTAGCCCT 
GGGGCTTCTC CAGGATCCGA GGCCCGGACC ACGGGTGACA TATCGGTGGA GAAGCTGAAC 
CTCGGCACTG ACTCGGACAG CTCACCTCAG AAGAGCTCGC GGGATCCTCC TTCCAGCCCA 
TCGAGCCTGA GCTCCCCCAT CCAGGAATCC ACTGCTCCCG AGCTGCCTTC GGAGACCCAG 
GAGACCCCAG GCCCCGCCCT GTGCAGCCCT CTGAGGAAGT CACCTCTGAC CCTCGAAGAT 
TTCAAGTTCC TGGCGGTGCT GGGCCGGGGT CATTTTGGGA AGGTGCTCCT CTCCGAATTC 
CGGCCCAGTG GGGAGCTGTT CGCCATCAAG GCTCTGAAGA AAGGGGACAT TGTGGCCCGA 
GACGAGGTGG AGAGCCTGAT GTGTGAGAAG CGGATATTGG CGGCAGTGAC CAGTGCGGGA 
CACCCCTTCC TGGTGAACCT CTTCGGCTGT TTCCAGACAC CGGAGCACGT GTGCTTCGTG 
ATGGAGTACT CGGCCGGTGG GGACCTGATG CTGCACATCC ACAGCGACGT GTTCTCTGAG 
CCCCGTGCCA TCTTTTATTC CGCCTGCGTG GTGCTGGGCC TACAGTTTCT TCACGAACAC 
AAGATCGTCT ACAGGGACCT GAAGTTGGAC AATTTGCTCC TGGACACCGA GGGCTACGTC 
AAGATCGCAG ACTTTGGCCT CTGCAAGGAG GGGATGGGCT ATGGGGACCG GACCAGCACA 
TTCTGTGGGA CCCCGGAGTT CCTGGCCCCT GAGGTGCTGA CGGACACGTC GTACACGCGA 
GCTGTGGACT GGTGGGGACT GGGTGTGCTG CTCTACGAGA TGCTGGTTGG CGAGTCCCCA 
TTCCCAGGGG ATGATGAGGA GGAGGTCTTC GACAGCATCG TCAACGACGA GGTTCGCTAC 
CCCCGCTTCC TGTCGGCCGA AGCCATCGGC ATCATGAGAA GGCTGCTTCG GAGGAACCCA 
GAGCGGAGGC TGGGATCTAG CGAGAGAGAT GCAGAAGATG TGAAGAAACA GCCCTTCTTC 
AGGACTCTGG GCTGGGAAGC CCTGTTGGCC CGGCGCCTGC CACCGCCCTT TGTGCCCACG 
CTGTCCGGCC GCACCGACGT CAGCAACTTC GACGAGGAGT TCACCGGGGA GGCCCCCACA 
CTGAGCCCGC CCCGCGACGC GCGGCCCCTC ACAGCCGCGG AGCAGGCAGC CTTCCTGGAC 
TTCGACTTCG TGGCCGGGGG CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998725.1
CDS	18..2864
Translation	MAEANNPSEQELESEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC

Target ORF information:

RefSeq Version	NM_213560.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase N1 (PKN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213560.1

ORF Insert Sequence:

ATGGCGGAGG CCAATAACCC CTCGGAGCAG GAGCTGGAGA GTGAGCCTCG CAGCTGGTCC 
CTGCTAGAGC AGCTGGGCCT GGCCGGGGCA GACCTGGCGG CCCCCGGGGT ACAGCAGCAG 
CTGGAGCTGG AGCGGGAGCG GCTGCGGCGG GAAATCCGCA AGGAGCTGAA GCTGAAGGAG 
GGTGCTGAGA ACCTGCGGCG GGCCACCACT GACCTGGGCC GCAGCCTGGG CCCCGTAGAG 
CTGCTGCTGC GGGGCTCCTC GCGCCGCCTC GACCTGCTGC ACCAGCAGCT GCAGGAGCTG 
CACGCCCACG TGGTGCTTCC CGACCCGGCG GCCACCCACG ATGGCCCCCA GTCCCCTGGT 
GCGGGTGGCC CCACCTGCTC GGCCACCAAC CTGAGCCGCG TGGCGGGCCT GGAGAAGCAG 
TTGGCCATTG AGCTGAAGGT GAAGCAGGGG GCGGAGAACA TGATCCAGAC CTACAGCAAT 
GGCAGCACCA AGGACCGGAA GCTGCTGCTG ACAGCCCAGC AGATGTTGCA GGACAGTAAG 
ACCAAGATTG ACATCATCCG CATGCAACTC CGCCGGGCGC TGCAGGCCGG CCAGCTGGAG 
AACCAGGCAG CCCCGGATGA CACCCAAGGG AGTCCTGACC TGGGGGCTGT GGAGCTGCGC 
ATCGAAGAGC TGCGGCACCA CTTCCGAGTG GAGCACGCGG TGGCCGAGGG TGCCAAGAAC 
GTACTGCGCC TGCTCAGCGC TGCCAAGGCC CCGGACCGCA AGGCAGTCAG CGAGGCCCAG 
GAGAAATTGA CAGAATCCAA CCAGAAGCTG GGGCTGCTGC GGGAGGCTCT GGAGCGGAGA 
CTTGGGGAGC TGCCCGCCGA CCACCCCAAG GGGCGGCTGC TGCGAGAAGA GCTCGCTGCG 
GCCTCCTCCG CTGCCTTCAG CACCCGCCTG GCCGGGCCCT TTCCCGCCAC GCACTACAGC 
ACCCTGTGCA AGCCCGCGCC GCTCACAGGG ACCCTGGAGG TACGAGTGGT GGGCTGCAGA 
GACCTCCCAG AGACCATCCC GTGGAACCCT ACCCCCTCAA TGGGGGGACC TGGGACCCCA 
GACAGCCGCC CCCCCTTCCT GAGCCGCCCA GCCCGGGGCC TTTACAGCCG AAGCGGAAGC 
CTCAGTGGCC GGAGCAGCCT CAAAGCAGAA GCCGAGAACA CCAGTGAAGT CAGCACTGTG 
CTTAAGCTGG ATAACACAGT GGTGGGGCAG ACGTCTTGGA AGCCATGTGG CCCCAATGCC 
TGGGACCAGA GCTTCACTCT GGAGCTGGAA AGGGCACGGG AACTGGAGTT GGCTGTGTTC 
TGGCGGGACC AGCGGGGCCT GTGTGCCCTC AAATTCCTGA AGTTGGAGGA TTTCTTGGAC 
AATGAGAGGC ATGAGGTGCA GCTGGACATG GAACCCCAGG GCTGCCTGGT GGCTGAGGTC 
ACCTTCCGCA ACCCTGTCAT TGAGAGGATT CCTCGGCTCC GACGGCAGAA GAAAATTTTC 
TCCAAGCAGC AAGGGAAGGC GTTCCAGCGT GCTAGGCAGA TGAACATCGA TGTCGCCACG 
TGGGTGCGGC TGCTCCGGAG GCTCATCCCC AATGCCACGG GCACAGGCAC CTTTAGCCCT 
GGGGCTTCTC CAGGATCCGA GGCCCGGACC ACGGGTGACA TATCGGTGGA GAAGCTGAAC 
CTCGGCACTG ACTCGGACAG CTCACCTCAG AAGAGCTCGC GGGATCCTCC TTCCAGCCCA 
TCGAGCCTGA GCTCCCCCAT CCAGGAATCC ACTGCTCCCG AGCTGCCTTC GGAGACCCAG 
GAGACCCCAG GCCCCGCCCT GTGCAGCCCT CTGAGGAAGT CACCTCTGAC CCTCGAAGAT 
TTCAAGTTCC TGGCGGTGCT GGGCCGGGGT CATTTTGGGA AGGTGCTCCT CTCCGAATTC 
CGGCCCAGTG GGGAGCTGTT CGCCATCAAG GCTCTGAAGA AAGGGGACAT TGTGGCCCGA 
GACGAGGTGG AGAGCCTGAT GTGTGAGAAG CGGATATTGG CGGCAGTGAC CAGTGCGGGA 
CACCCCTTCC TGGTGAACCT CTTCGGCTGT TTCCAGACAC CGGAGCACGT GTGCTTCGTG 
ATGGAGTACT CGGCCGGTGG GGACCTGATG CTGCACATCC ACAGCGACGT GTTCTCTGAG 
CCCCGTGCCA TCTTTTATTC CGCCTGCGTG GTGCTGGGCC TACAGTTTCT TCACGAACAC 
AAGATCGTCT ACAGGGACCT GAAGTTGGAC AATTTGCTCC TGGACACCGA GGGCTACGTC 
AAGATCGCAG ACTTTGGCCT CTGCAAGGAG GGGATGGGCT ATGGGGACCG GACCAGCACA 
TTCTGTGGGA CCCCGGAGTT CCTGGCCCCT GAGGTGCTGA CGGACACGTC GTACACGCGA 
GCTGTGGACT GGTGGGGACT GGGTGTGCTG CTCTACGAGA TGCTGGTTGG CGAGTCCCCA 
TTCCCAGGGG ATGATGAGGA GGAGGTCTTC GACAGCATCG TCAACGACGA GGTTCGCTAC 
CCCCGCTTCC TGTCGGCCGA AGCCATCGGC ATCATGAGAA GGCTGCTTCG GAGGAACCCA 
GAGCGGAGGC TGGGATCTAG CGAGAGAGAT GCAGAAGATG TGAAGAAACA GCCCTTCTTC 
AGGACTCTGG GCTGGGAAGC CCTGTTGGCC CGGCGCCTGC CACCGCCCTT TGTGCCCACG 
CTGTCCGGCC GCACCGACGT CAGCAACTTC GACGAGGAGT TCACCGGGGA GGCCCCCACA 
CTGAGCCCGC CCCGCGACGC GCGGCCCCTC ACAGCCGCGG AGCAGGCAGC CTTCCTGGAC 
TTCGACTTCG TGGCCGGGGG CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PKN1 ( XM_011528128.1 ) cDNA ORF clone, Homo sapiens(human) -> XP_011526430.1 Homo sapiens protein kinase N1 (PKN1), transcript variant X1, mRNA.

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CloneID	OHu59922
Clone ID Related Accession (Same CDS sequence)	XM_011528128.1
Accession Version	XM_011528128.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2865bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-25
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase N1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000019.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Homo sapiens Annotation Release 109 Annotation Version :: 109 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTGCC GGCCTTGCCG AGGAGCTGGG CGTATCTGGT GTTGCCGATC ACACAAGAGT 
GAGCCTCGCA GCTGGTCCCT GCTAGAGCAG CTGGGCCTGG CCGGGGCAGA CCTGGCGGCC 
CCCGGGGTAC AGCAGCAGCT GGAGCTGGAG CGGGAGCGGC TGCGGCGGGA AATCCGCAAG 
GAGCTGAAGC TGAAGGAGGG TGCTGAGAAC CTGCGGCGGG CCACCACTGA CCTGGGCCGC 
AGCCTGGGCC CCGTAGAGCT GCTGCTGCGG GGCTCCTCGC GCCGCCTCGA CCTGCTGCAC 
CAGCAGCTGC AGGAGCTGCA CGCCCACGTG GTGCTTCCCG ACCCGGCGGC CACCCACGAT 
GGCCCCCAGT CCCCTGGTGC GGGTGGCCCC ACCTGCTCGG CCACCAACCT GAGCCGCGTG 
GCGGGCCTGG AGAAGCAGTT GGCCATTGAG CTGAAGGTGA AGCAGGGGGC GGAGAACATG 
ATCCAGACCT ACAGCAATGG CAGCACCAAG GACCGGAAGC TGCTGCTGAC AGCCCAGCAG 
ATGTTGCAGG ACAGTAAGAC CAAGATTGAC ATCATCCGCA TGCAACTCCG CCGGGCGCTG 
CAGGCCGGCC AGCTGGAGAA CCAGGCAGCC CCGGATGACA CCCAAGGGAG TCCTGACCTG 
GGGGCTGTGG AGCTGCGCAT CGAAGAGCTG CGGCACCACT TCCGAGTGGA GCACGCGGTG 
GCCGAGGGTG CCAAGAACGT ACTGCGCCTG CTCAGCGCTG CCAAGGCCCC GGACCGCAAG 
GCAGTCAGCG AGGCCCAGGA GAAATTAACA GAATCCAACC AGAAGCTGGG GCTGCTGCGG 
GAGGCTCTGG AGCGGAGACT TGGGGAGCTG CCCGCCGACC ACCCCAAGGG GCGGCTGCTG 
CGAGAAGAGC TCGCTGCGGC CTCCTCCGCT GCCTTCAGCA CCCGCCTGGC CGGGCCCTTT 
CCCGCCACGC ACTACAGCAC CCTGTGCAAG CCCGCGCCGC TCACAGGGAC CCTGGAGGTA 
CGAGTGGTGG GCTGCAGAGA CCTCCCAGAG ACCATCCCGT GGAACCCTAC CCCCTCAATG 
GGGGGACCTG GGACCCCAGA CAGCCGCCCC CCCTTCCTGA GCCGCCCAGC CCGGGGCCTT 
TACAGCCGAA GCGGAAGCCT CAGTGGCCGG AGCAGCCTCA AAGCAGAAGC CGAGAACACC 
AGTGAAGTCA GCACTGTGCT TAAGCTGGAT AACACAGTGG TGGGGCAGAC GTCTTGGAAG 
CCATGTGGCC CCAATGCCTG GGACCAGAGC TTCACTCTGG AGCTGGAAAG GGCACGGGAA 
CTGGAGTTGG CTGTGTTCTG GCGGGACCAG CGGGGCCTGT GTGCCCTCAA ATTCCTGAAG 
TTGGAGGATT TCTTGGACAA TGAGAGGCAT GAGGTGCAGC TGGACATGGA ACCCCAGGGC 
TGCCTGGTGG CTGAGGTCAC CTTCCGCAAC CCTGTCATTG AGAGGATTCC TCGGCTCCGA 
CGGCAGAAGA AAATTTTCTC CAAGCAGCAA GGGAAGGCGT TCCAGCGTGC TAGGCAGATG 
AACATCGATG TCGCCACGTG GGTGCGGCTG CTCCGGAGGC TCATCCCCAA TGCCACGGGC 
ACAGGCACCT TTAGCCCTGG GGCTTCTCCA GGATCCGAGG CCCGGACCAC GGGTGACATA 
TCGGTGGAGA AGCTGAACCT CGGCACTGAC TCGGACAGCT CACCTCAGAA GAGCTCGCGG 
GATCCTCCTT CCAGCCCATC GAGCCTGAGC TCCCCCATCC AGGAATCCAC TGCTCCCGAG 
CTGCCTTCGG AGACCCAGGA GACCCCAGGC CCCGCCCTGT GCAGCCCTCT GAGGAAGTCA 
CCTCTGACCC TCGAAGATTT CAAGTTCCTG GCGGTGCTGG GCCGGGGTCA TTTTGGGAAG 
GTGCTCCTCT CCGAATTCCG GCCCAGTGGG GAGCTGTTCG CCATCAAGGC TCTGAAGAAA 
GGGGACATTG TGGCCCGAGA CGAGGTGGAG AGCCTGATGT GTGAGAAGCG GATATTGGCG 
GCAGTGACCA GTGCGGGACA CCCCTTCCTG GTGAACCTCT TCGGCTGTTT CCAGACACCG 
GAGCACGTGT GCTTCGTGAT GGAGTACTCG GCCGGTGGGG ACCTGATGCT GCACATCCAC 
AGCGACGTGT TCTCTGAGCC CCGTGCCATC TTTTATTCCG CCTGCGTGGT GCTGGGCCTA 
CAGTTTCTTC ACGAACACAA GATCGTCTAC AGGGACCTGA AGTTGGACAA TTTGCTCCTG 
GACACCGAGG GCTACGTCAA GATCGCAGAC TTTGGCCTCT GCAAGGAGGG GATGGGCTAT 
GGGGACCGGA CCAGCACATT CTGTGGGACC CCGGAGTTCC TGGCCCCTGA GGTGCTGACG 
GACACGTCGT ACACGCGAGC TGTGGACTGG TGGGGACTGG GTGTGCTGCT CTACGAGATG 
CTGGTTGGCG AGTCCCCATT CCCAGGGGAT GATGAGGAGG AGGTCTTCGA CAGCATCGTC 
AACGACGAGG TTCGCTACCC CCGCTTCCTG TCGGCCGAAG CCATCGGCAT CATGAGAAGG 
CTGCTTCGGA GGAACCCAGA GCGGAGGCTG GGATCTAGCG AGAGAGATGC AGAAGATGTG 
AAGAAACAGC CCTTCTTCAG GACTCTGGGC TGGGAAGCCC TGTTGGCCCG GCGCCTGCCA 
CCGCCCTTTG TGCCCACGCT GTCCGGCCGC ACCGACGTCA GCAACTTCGA CGAGGAGTTC 
ACCGGGGAGG CCCCCACACT GAGCCCGCCC CGCGACGCGC GGCCCCTCAC AGCCGCGGAG 
CAGGCAGCCT TCCTGGACTT CGACTTCGTG GCCGGGGGCT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011526430.1
CDS	16..2880
Translation	MACRPCRGAGRIWCCRSHKSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC

Target ORF information:

RefSeq Version	XM_011528128.1
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase N1 (PKN1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011528128.1

ORF Insert Sequence:

ATGGCCTGCC GGCCTTGCCG AGGAGCTGGG CGTATCTGGT GTTGCCGATC ACACAAGAGT 
GAGCCTCGCA GCTGGTCCCT GCTAGAGCAG CTGGGCCTGG CCGGGGCAGA CCTGGCGGCC 
CCCGGGGTAC AGCAGCAGCT GGAGCTGGAG CGGGAGCGGC TGCGGCGGGA AATCCGCAAG 
GAGCTGAAGC TGAAGGAGGG TGCTGAGAAC CTGCGGCGGG CCACCACTGA CCTGGGCCGC 
AGCCTGGGCC CCGTAGAGCT GCTGCTGCGG GGCTCCTCGC GCCGCCTCGA CCTGCTGCAC 
CAGCAGCTGC AGGAGCTGCA CGCCCACGTG GTGCTTCCCG ACCCGGCGGC CACCCACGAT 
GGCCCCCAGT CCCCTGGTGC GGGTGGCCCC ACCTGCTCGG CCACCAACCT GAGCCGCGTG 
GCGGGCCTGG AGAAGCAGTT GGCCATTGAG CTGAAGGTGA AGCAGGGGGC GGAGAACATG 
ATCCAGACCT ACAGCAATGG CAGCACCAAG GACCGGAAGC TGCTGCTGAC AGCCCAGCAG 
ATGTTGCAGG ACAGTAAGAC CAAGATTGAC ATCATCCGCA TGCAACTCCG CCGGGCGCTG 
CAGGCCGGCC AGCTGGAGAA CCAGGCAGCC CCGGATGACA CCCAAGGGAG TCCTGACCTG 
GGGGCTGTGG AGCTGCGCAT CGAAGAGCTG CGGCACCACT TCCGAGTGGA GCACGCGGTG 
GCCGAGGGTG CCAAGAACGT ACTGCGCCTG CTCAGCGCTG CCAAGGCCCC GGACCGCAAG 
GCAGTCAGCG AGGCCCAGGA GAAATTAACA GAATCCAACC AGAAGCTGGG GCTGCTGCGG 
GAGGCTCTGG AGCGGAGACT TGGGGAGCTG CCCGCCGACC ACCCCAAGGG GCGGCTGCTG 
CGAGAAGAGC TCGCTGCGGC CTCCTCCGCT GCCTTCAGCA CCCGCCTGGC CGGGCCCTTT 
CCCGCCACGC ACTACAGCAC CCTGTGCAAG CCCGCGCCGC TCACAGGGAC CCTGGAGGTA 
CGAGTGGTGG GCTGCAGAGA CCTCCCAGAG ACCATCCCGT GGAACCCTAC CCCCTCAATG 
GGGGGACCTG GGACCCCAGA CAGCCGCCCC CCCTTCCTGA GCCGCCCAGC CCGGGGCCTT 
TACAGCCGAA GCGGAAGCCT CAGTGGCCGG AGCAGCCTCA AAGCAGAAGC CGAGAACACC 
AGTGAAGTCA GCACTGTGCT TAAGCTGGAT AACACAGTGG TGGGGCAGAC GTCTTGGAAG 
CCATGTGGCC CCAATGCCTG GGACCAGAGC TTCACTCTGG AGCTGGAAAG GGCACGGGAA 
CTGGAGTTGG CTGTGTTCTG GCGGGACCAG CGGGGCCTGT GTGCCCTCAA ATTCCTGAAG 
TTGGAGGATT TCTTGGACAA TGAGAGGCAT GAGGTGCAGC TGGACATGGA ACCCCAGGGC 
TGCCTGGTGG CTGAGGTCAC CTTCCGCAAC CCTGTCATTG AGAGGATTCC TCGGCTCCGA 
CGGCAGAAGA AAATTTTCTC CAAGCAGCAA GGGAAGGCGT TCCAGCGTGC TAGGCAGATG 
AACATCGATG TCGCCACGTG GGTGCGGCTG CTCCGGAGGC TCATCCCCAA TGCCACGGGC 
ACAGGCACCT TTAGCCCTGG GGCTTCTCCA GGATCCGAGG CCCGGACCAC GGGTGACATA 
TCGGTGGAGA AGCTGAACCT CGGCACTGAC TCGGACAGCT CACCTCAGAA GAGCTCGCGG 
GATCCTCCTT CCAGCCCATC GAGCCTGAGC TCCCCCATCC AGGAATCCAC TGCTCCCGAG 
CTGCCTTCGG AGACCCAGGA GACCCCAGGC CCCGCCCTGT GCAGCCCTCT GAGGAAGTCA 
CCTCTGACCC TCGAAGATTT CAAGTTCCTG GCGGTGCTGG GCCGGGGTCA TTTTGGGAAG 
GTGCTCCTCT CCGAATTCCG GCCCAGTGGG GAGCTGTTCG CCATCAAGGC TCTGAAGAAA 
GGGGACATTG TGGCCCGAGA CGAGGTGGAG AGCCTGATGT GTGAGAAGCG GATATTGGCG 
GCAGTGACCA GTGCGGGACA CCCCTTCCTG GTGAACCTCT TCGGCTGTTT CCAGACACCG 
GAGCACGTGT GCTTCGTGAT GGAGTACTCG GCCGGTGGGG ACCTGATGCT GCACATCCAC 
AGCGACGTGT TCTCTGAGCC CCGTGCCATC TTTTATTCCG CCTGCGTGGT GCTGGGCCTA 
CAGTTTCTTC ACGAACACAA GATCGTCTAC AGGGACCTGA AGTTGGACAA TTTGCTCCTG 
GACACCGAGG GCTACGTCAA GATCGCAGAC TTTGGCCTCT GCAAGGAGGG GATGGGCTAT 
GGGGACCGGA CCAGCACATT CTGTGGGACC CCGGAGTTCC TGGCCCCTGA GGTGCTGACG 
GACACGTCGT ACACGCGAGC TGTGGACTGG TGGGGACTGG GTGTGCTGCT CTACGAGATG 
CTGGTTGGCG AGTCCCCATT CCCAGGGGAT GATGAGGAGG AGGTCTTCGA CAGCATCGTC 
AACGACGAGG TTCGCTACCC CCGCTTCCTG TCGGCCGAAG CCATCGGCAT CATGAGAAGG 
CTGCTTCGGA GGAACCCAGA GCGGAGGCTG GGATCTAGCG AGAGAGATGC AGAAGATGTG 
AAGAAACAGC CCTTCTTCAG GACTCTGGGC TGGGAAGCCC TGTTGGCCCG GCGCCTGCCA 
CCGCCCTTTG TGCCCACGCT GTCCGGCCGC ACCGACGTCA GCAACTTCGA CGAGGAGTTC 
ACCGGGGAGG CCCCCACACT GAGCCCGCCC CGCGACGCGC GGCCCCTCAC AGCCGCGGAG 
CAGGCAGCCT TCCTGGACTT CGACTTCGTG GCCGGGGGCT GCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PKN1 ( NM_213560.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_998725.1 Homo sapiens protein kinase N1 (PKN1), transcript variant 1, mRNA.

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CloneID	OHu107138
Clone ID Related Accession (Same CDS sequence)	NM_213560.2 , NM_213560.3
Accession Version	NM_213560.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-03
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase N1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008569.7. On Aug 25, 2018 this sequence version replaced NM_213560.1. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292130.1, AK123007.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CCAATAACCC CTCGGAGCAG GAGCTGGAGA GTGAGCCTCG CAGCTGGTCC 
CTGCTAGAGC AGCTGGGCCT GGCCGGGGCA GACCTGGCGG CCCCCGGGGT ACAGCAGCAG 
CTGGAGCTGG AGCGGGAGCG GCTGCGGCGG GAAATCCGCA AGGAGCTGAA GCTGAAGGAG 
GGTGCTGAGA ACCTGCGGCG GGCCACCACT GACCTGGGCC GCAGCCTGGG CCCCGTAGAG 
CTGCTGCTGC GGGGCTCCTC GCGCCGCCTC GACCTGCTGC ACCAGCAGCT GCAGGAGCTG 
CACGCCCACG TGGTGCTTCC CGACCCGGCG GCCACCCACG ATGGCCCCCA GTCCCCTGGT 
GCGGGTGGCC CCACCTGCTC GGCCACCAAC CTGAGCCGCG TGGCGGGCCT GGAGAAGCAG 
TTGGCCATTG AGCTGAAGGT GAAGCAGGGG GCGGAGAACA TGATCCAGAC CTACAGCAAT 
GGCAGCACCA AGGACCGGAA GCTGCTGCTG ACAGCCCAGC AGATGTTGCA GGACAGTAAG 
ACCAAGATTG ACATCATCCG CATGCAACTC CGCCGGGCGC TGCAGGCCGG CCAGCTGGAG 
AACCAGGCAG CCCCGGATGA CACCCAAGGG AGTCCTGACC TGGGGGCTGT GGAGCTGCGC 
ATCGAAGAGC TGCGGCACCA CTTCCGAGTG GAGCACGCGG TGGCCGAGGG TGCCAAGAAC 
GTACTGCGCC TGCTCAGCGC TGCCAAGGCC CCGGACCGCA AGGCAGTCAG CGAGGCCCAG 
GAGAAATTAA CAGAATCCAA CCAGAAGCTG GGGCTGCTGC GGGAGGCTCT GGAGCGGAGA 
CTTGGGGAGC TGCCCGCCGA CCACCCCAAG GGGCGGCTGC TGCGAGAAGA GCTCGCTGCG 
GCCTCCTCCG CTGCCTTCAG CACCCGCCTG GCCGGGCCCT TTCCCGCCAC GCACTACAGC 
ACCCTGTGCA AGCCCGCGCC GCTCACAGGG ACCCTGGAGG TACGAGTGGT GGGCTGCAGA 
GACCTCCCAG AGACCATCCC GTGGAACCCT ACCCCCTCAA TGGGGGGACC TGGGACCCCA 
GACAGCCGCC CCCCCTTCCT GAGCCGCCCA GCCCGGGGCC TTTACAGCCG AAGCGGAAGC 
CTCAGTGGCC GGAGCAGCCT CAAAGCAGAA GCCGAGAACA CCAGTGAAGT CAGCACTGTG 
CTTAAGCTGG ATAACACAGT GGTGGGGCAG ACGTCTTGGA AGCCATGTGG CCCCAATGCC 
TGGGACCAGA GCTTCACTCT GGAGCTGGAA AGGGCACGGG AACTGGAGTT GGCTGTGTTC 
TGGCGGGACC AGCGGGGCCT GTGTGCCCTC AAATTCCTGA AGTTGGAGGA TTTCTTGGAC 
AATGAGAGGC ATGAGGTGCA GCTGGACATG GAACCCCAGG GCTGCCTGGT GGCTGAGGTC 
ACCTTCCGCA ACCCTGTCAT TGAGAGGATT CCTCGGCTCC GACGGCAGAA GAAAATTTTC 
TCCAAGCAGC AAGGGAAGGC GTTCCAGCGT GCTAGGCAGA TGAACATCGA TGTCGCCACG 
TGGGTGCGGC TGCTCCGGAG GCTCATCCCC AATGCCACGG GCACAGGCAC CTTTAGCCCT 
GGGGCTTCTC CAGGATCCGA GGCCCGGACC ACGGGTGACA TATCGGTGGA GAAGCTGAAC 
CTCGGCACTG ACTCGGACAG CTCACCTCAG AAGAGCTCGC GGGATCCTCC TTCCAGCCCA 
TCGAGCCTGA GCTCCCCCAT CCAGGAATCC ACTGCTCCCG AGCTGCCTTC GGAGACCCAG 
GAGACCCCAG GCCCCGCCCT GTGCAGCCCT CTGAGGAAGT CACCTCTGAC CCTCGAAGAT 
TTCAAGTTCC TGGCGGTGCT GGGCCGGGGT CATTTTGGGA AGGTGCTCCT CTCCGAATTC 
CGGCCCAGTG GGGAGCTGTT CGCCATCAAG GCTCTGAAGA AAGGGGACAT TGTGGCCCGA 
GACGAGGTGG AGAGCCTGAT GTGTGAGAAG CGGATATTGG CGGCAGTGAC CAGTGCGGGA 
CACCCCTTCC TGGTGAACCT CTTCGGCTGT TTCCAGACAC CGGAGCACGT GTGCTTCGTG 
ATGGAGTACT CGGCCGGTGG GGACCTGATG CTGCACATCC ACAGCGACGT GTTCTCTGAG 
CCCCGTGCCA TCTTTTATTC CGCCTGCGTG GTGCTGGGCC TACAGTTTCT TCACGAACAC 
AAGATCGTCT ACAGGGACCT GAAGTTGGAC AATTTGCTCC TGGACACCGA GGGCTACGTC 
AAGATCGCAG ACTTTGGCCT CTGCAAGGAG GGGATGGGCT ATGGGGACCG GACCAGCACA 
TTCTGTGGGA CCCCGGAGTT CCTGGCCCCT GAGGTGCTGA CGGACACGTC GTACACGCGA 
GCTGTGGACT GGTGGGGACT GGGTGTGCTG CTCTACGAGA TGCTGGTTGG CGAGTCCCCA 
TTCCCAGGGG ATGATGAGGA GGAGGTCTTC GACAGCATCG TCAACGACGA GGTTCGCTAC 
CCCCGCTTCC TGTCGGCCGA AGCCATCGGC ATCATGAGAA GGCTGCTTCG GAGGAACCCA 
GAGCGGAGGC TGGGATCTAG CGAGAGAGAT GCAGAAGATG TGAAGAAACA GCCCTTCTTC 
AGGACTCTGG GCTGGGAAGC CCTGTTGGCC CGGCGCCTGC CACCGCCCTT TGTGCCCACG 
CTGTCCGGCC GCACCGACGT CAGCAACTTC GACGAGGAGT TCACCGGGGA GGCCCCCACA 
CTGAGCCCGC CCCGCGACGC GCGGCCCCTC ACAGCCGCGG AGCAGGCAGC CTTCCTGGAC 
TTCGACTTCG TGGCCGGGGG CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998725.1
CDS	15..2861
Translation	MAEANNPSEQELESEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC

Target ORF information:

RefSeq Version	NM_213560.2
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase N1 (PKN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213560.2

ORF Insert Sequence:

ATGGCGGAGG CCAATAACCC CTCGGAGCAG GAGCTGGAGA GTGAGCCTCG CAGCTGGTCC 
CTGCTAGAGC AGCTGGGCCT GGCCGGGGCA GACCTGGCGG CCCCCGGGGT ACAGCAGCAG 
CTGGAGCTGG AGCGGGAGCG GCTGCGGCGG GAAATCCGCA AGGAGCTGAA GCTGAAGGAG 
GGTGCTGAGA ACCTGCGGCG GGCCACCACT GACCTGGGCC GCAGCCTGGG CCCCGTAGAG 
CTGCTGCTGC GGGGCTCCTC GCGCCGCCTC GACCTGCTGC ACCAGCAGCT GCAGGAGCTG 
CACGCCCACG TGGTGCTTCC CGACCCGGCG GCCACCCACG ATGGCCCCCA GTCCCCTGGT 
GCGGGTGGCC CCACCTGCTC GGCCACCAAC CTGAGCCGCG TGGCGGGCCT GGAGAAGCAG 
TTGGCCATTG AGCTGAAGGT GAAGCAGGGG GCGGAGAACA TGATCCAGAC CTACAGCAAT 
GGCAGCACCA AGGACCGGAA GCTGCTGCTG ACAGCCCAGC AGATGTTGCA GGACAGTAAG 
ACCAAGATTG ACATCATCCG CATGCAACTC CGCCGGGCGC TGCAGGCCGG CCAGCTGGAG 
AACCAGGCAG CCCCGGATGA CACCCAAGGG AGTCCTGACC TGGGGGCTGT GGAGCTGCGC 
ATCGAAGAGC TGCGGCACCA CTTCCGAGTG GAGCACGCGG TGGCCGAGGG TGCCAAGAAC 
GTACTGCGCC TGCTCAGCGC TGCCAAGGCC CCGGACCGCA AGGCAGTCAG CGAGGCCCAG 
GAGAAATTAA CAGAATCCAA CCAGAAGCTG GGGCTGCTGC GGGAGGCTCT GGAGCGGAGA 
CTTGGGGAGC TGCCCGCCGA CCACCCCAAG GGGCGGCTGC TGCGAGAAGA GCTCGCTGCG 
GCCTCCTCCG CTGCCTTCAG CACCCGCCTG GCCGGGCCCT TTCCCGCCAC GCACTACAGC 
ACCCTGTGCA AGCCCGCGCC GCTCACAGGG ACCCTGGAGG TACGAGTGGT GGGCTGCAGA 
GACCTCCCAG AGACCATCCC GTGGAACCCT ACCCCCTCAA TGGGGGGACC TGGGACCCCA 
GACAGCCGCC CCCCCTTCCT GAGCCGCCCA GCCCGGGGCC TTTACAGCCG AAGCGGAAGC 
CTCAGTGGCC GGAGCAGCCT CAAAGCAGAA GCCGAGAACA CCAGTGAAGT CAGCACTGTG 
CTTAAGCTGG ATAACACAGT GGTGGGGCAG ACGTCTTGGA AGCCATGTGG CCCCAATGCC 
TGGGACCAGA GCTTCACTCT GGAGCTGGAA AGGGCACGGG AACTGGAGTT GGCTGTGTTC 
TGGCGGGACC AGCGGGGCCT GTGTGCCCTC AAATTCCTGA AGTTGGAGGA TTTCTTGGAC 
AATGAGAGGC ATGAGGTGCA GCTGGACATG GAACCCCAGG GCTGCCTGGT GGCTGAGGTC 
ACCTTCCGCA ACCCTGTCAT TGAGAGGATT CCTCGGCTCC GACGGCAGAA GAAAATTTTC 
TCCAAGCAGC AAGGGAAGGC GTTCCAGCGT GCTAGGCAGA TGAACATCGA TGTCGCCACG 
TGGGTGCGGC TGCTCCGGAG GCTCATCCCC AATGCCACGG GCACAGGCAC CTTTAGCCCT 
GGGGCTTCTC CAGGATCCGA GGCCCGGACC ACGGGTGACA TATCGGTGGA GAAGCTGAAC 
CTCGGCACTG ACTCGGACAG CTCACCTCAG AAGAGCTCGC GGGATCCTCC TTCCAGCCCA 
TCGAGCCTGA GCTCCCCCAT CCAGGAATCC ACTGCTCCCG AGCTGCCTTC GGAGACCCAG 
GAGACCCCAG GCCCCGCCCT GTGCAGCCCT CTGAGGAAGT CACCTCTGAC CCTCGAAGAT 
TTCAAGTTCC TGGCGGTGCT GGGCCGGGGT CATTTTGGGA AGGTGCTCCT CTCCGAATTC 
CGGCCCAGTG GGGAGCTGTT CGCCATCAAG GCTCTGAAGA AAGGGGACAT TGTGGCCCGA 
GACGAGGTGG AGAGCCTGAT GTGTGAGAAG CGGATATTGG CGGCAGTGAC CAGTGCGGGA 
CACCCCTTCC TGGTGAACCT CTTCGGCTGT TTCCAGACAC CGGAGCACGT GTGCTTCGTG 
ATGGAGTACT CGGCCGGTGG GGACCTGATG CTGCACATCC ACAGCGACGT GTTCTCTGAG 
CCCCGTGCCA TCTTTTATTC CGCCTGCGTG GTGCTGGGCC TACAGTTTCT TCACGAACAC 
AAGATCGTCT ACAGGGACCT GAAGTTGGAC AATTTGCTCC TGGACACCGA GGGCTACGTC 
AAGATCGCAG ACTTTGGCCT CTGCAAGGAG GGGATGGGCT ATGGGGACCG GACCAGCACA 
TTCTGTGGGA CCCCGGAGTT CCTGGCCCCT GAGGTGCTGA CGGACACGTC GTACACGCGA 
GCTGTGGACT GGTGGGGACT GGGTGTGCTG CTCTACGAGA TGCTGGTTGG CGAGTCCCCA 
TTCCCAGGGG ATGATGAGGA GGAGGTCTTC GACAGCATCG TCAACGACGA GGTTCGCTAC 
CCCCGCTTCC TGTCGGCCGA AGCCATCGGC ATCATGAGAA GGCTGCTTCG GAGGAACCCA 
GAGCGGAGGC TGGGATCTAG CGAGAGAGAT GCAGAAGATG TGAAGAAACA GCCCTTCTTC 
AGGACTCTGG GCTGGGAAGC CCTGTTGGCC CGGCGCCTGC CACCGCCCTT TGTGCCCACG 
CTGTCCGGCC GCACCGACGT CAGCAACTTC GACGAGGAGT TCACCGGGGA GGCCCCCACA 
CTGAGCCCGC CCCGCGACGC GCGGCCCCTC ACAGCCGCGG AGCAGGCAGC CTTCCTGGAC 
TTCGACTTCG TGGCCGGGGG CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PKN1 ( NM_213560.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_998725.1 Homo sapiens protein kinase N1 (PKN1), transcript variant 1, mRNA.

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CloneID	OHu107138
Clone ID Related Accession (Same CDS sequence)	NM_213560.2 , NM_213560.3
Accession Version	NM_213560.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2847bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-23
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase N1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008569.7. On Jun 2, 2019 this sequence version replaced NM_213560.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK292130.1, AK123007.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968189, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGGAGG CCAATAACCC CTCGGAGCAG GAGCTGGAGA GTGAGCCTCG CAGCTGGTCC 
CTGCTAGAGC AGCTGGGCCT GGCCGGGGCA GACCTGGCGG CCCCCGGGGT ACAGCAGCAG 
CTGGAGCTGG AGCGGGAGCG GCTGCGGCGG GAAATCCGCA AGGAGCTGAA GCTGAAGGAG 
GGTGCTGAGA ACCTGCGGCG GGCCACCACT GACCTGGGCC GCAGCCTGGG CCCCGTAGAG 
CTGCTGCTGC GGGGCTCCTC GCGCCGCCTC GACCTGCTGC ACCAGCAGCT GCAGGAGCTG 
CACGCCCACG TGGTGCTTCC CGACCCGGCG GCCACCCACG ATGGCCCCCA GTCCCCTGGT 
GCGGGTGGCC CCACCTGCTC GGCCACCAAC CTGAGCCGCG TGGCGGGCCT GGAGAAGCAG 
TTGGCCATTG AGCTGAAGGT GAAGCAGGGG GCGGAGAACA TGATCCAGAC CTACAGCAAT 
GGCAGCACCA AGGACCGGAA GCTGCTGCTG ACAGCCCAGC AGATGTTGCA GGACAGTAAG 
ACCAAGATTG ACATCATCCG CATGCAACTC CGCCGGGCGC TGCAGGCCGG CCAGCTGGAG 
AACCAGGCAG CCCCGGATGA CACCCAAGGG AGTCCTGACC TGGGGGCTGT GGAGCTGCGC 
ATCGAAGAGC TGCGGCACCA CTTCCGAGTG GAGCACGCGG TGGCCGAGGG TGCCAAGAAC 
GTACTGCGCC TGCTCAGCGC TGCCAAGGCC CCGGACCGCA AGGCAGTCAG CGAGGCCCAG 
GAGAAATTAA CAGAATCCAA CCAGAAGCTG GGGCTGCTGC GGGAGGCTCT GGAGCGGAGA 
CTTGGGGAGC TGCCCGCCGA CCACCCCAAG GGGCGGCTGC TGCGAGAAGA GCTCGCTGCG 
GCCTCCTCCG CTGCCTTCAG CACCCGCCTG GCCGGGCCCT TTCCCGCCAC GCACTACAGC 
ACCCTGTGCA AGCCCGCGCC GCTCACAGGG ACCCTGGAGG TACGAGTGGT GGGCTGCAGA 
GACCTCCCAG AGACCATCCC GTGGAACCCT ACCCCCTCAA TGGGGGGACC TGGGACCCCA 
GACAGCCGCC CCCCCTTCCT GAGCCGCCCA GCCCGGGGCC TTTACAGCCG AAGCGGAAGC 
CTCAGTGGCC GGAGCAGCCT CAAAGCAGAA GCCGAGAACA CCAGTGAAGT CAGCACTGTG 
CTTAAGCTGG ATAACACAGT GGTGGGGCAG ACGTCTTGGA AGCCATGTGG CCCCAATGCC 
TGGGACCAGA GCTTCACTCT GGAGCTGGAA AGGGCACGGG AACTGGAGTT GGCTGTGTTC 
TGGCGGGACC AGCGGGGCCT GTGTGCCCTC AAATTCCTGA AGTTGGAGGA TTTCTTGGAC 
AATGAGAGGC ATGAGGTGCA GCTGGACATG GAACCCCAGG GCTGCCTGGT GGCTGAGGTC 
ACCTTCCGCA ACCCTGTCAT TGAGAGGATT CCTCGGCTCC GACGGCAGAA GAAAATTTTC 
TCCAAGCAGC AAGGGAAGGC GTTCCAGCGT GCTAGGCAGA TGAACATCGA TGTCGCCACG 
TGGGTGCGGC TGCTCCGGAG GCTCATCCCC AATGCCACGG GCACAGGCAC CTTTAGCCCT 
GGGGCTTCTC CAGGATCCGA GGCCCGGACC ACGGGTGACA TATCGGTGGA GAAGCTGAAC 
CTCGGCACTG ACTCGGACAG CTCACCTCAG AAGAGCTCGC GGGATCCTCC TTCCAGCCCA 
TCGAGCCTGA GCTCCCCCAT CCAGGAATCC ACTGCTCCCG AGCTGCCTTC GGAGACCCAG 
GAGACCCCAG GCCCCGCCCT GTGCAGCCCT CTGAGGAAGT CACCTCTGAC CCTCGAAGAT 
TTCAAGTTCC TGGCGGTGCT GGGCCGGGGT CATTTTGGGA AGGTGCTCCT CTCCGAATTC 
CGGCCCAGTG GGGAGCTGTT CGCCATCAAG GCTCTGAAGA AAGGGGACAT TGTGGCCCGA 
GACGAGGTGG AGAGCCTGAT GTGTGAGAAG CGGATATTGG CGGCAGTGAC CAGTGCGGGA 
CACCCCTTCC TGGTGAACCT CTTCGGCTGT TTCCAGACAC CGGAGCACGT GTGCTTCGTG 
ATGGAGTACT CGGCCGGTGG GGACCTGATG CTGCACATCC ACAGCGACGT GTTCTCTGAG 
CCCCGTGCCA TCTTTTATTC CGCCTGCGTG GTGCTGGGCC TACAGTTTCT TCACGAACAC 
AAGATCGTCT ACAGGGACCT GAAGTTGGAC AATTTGCTCC TGGACACCGA GGGCTACGTC 
AAGATCGCAG ACTTTGGCCT CTGCAAGGAG GGGATGGGCT ATGGGGACCG GACCAGCACA 
TTCTGTGGGA CCCCGGAGTT CCTGGCCCCT GAGGTGCTGA CGGACACGTC GTACACGCGA 
GCTGTGGACT GGTGGGGACT GGGTGTGCTG CTCTACGAGA TGCTGGTTGG CGAGTCCCCA 
TTCCCAGGGG ATGATGAGGA GGAGGTCTTC GACAGCATCG TCAACGACGA GGTTCGCTAC 
CCCCGCTTCC TGTCGGCCGA AGCCATCGGC ATCATGAGAA GGCTGCTTCG GAGGAACCCA 
GAGCGGAGGC TGGGATCTAG CGAGAGAGAT GCAGAAGATG TGAAGAAACA GCCCTTCTTC 
AGGACTCTGG GCTGGGAAGC CCTGTTGGCC CGGCGCCTGC CACCGCCCTT TGTGCCCACG 
CTGTCCGGCC GCACCGACGT CAGCAACTTC GACGAGGAGT TCACCGGGGA GGCCCCCACA 
CTGAGCCCGC CCCGCGACGC GCGGCCCCTC ACAGCCGCGG AGCAGGCAGC CTTCCTGGAC 
TTCGACTTCG TGGCCGGGGG CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998725.1
CDS	15..2861
Translation	MAEANNPSEQELESEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC

Target ORF information:

RefSeq Version	NM_213560.3
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase N1 (PKN1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213560.3

ORF Insert Sequence:

ATGGCGGAGG CCAATAACCC CTCGGAGCAG GAGCTGGAGA GTGAGCCTCG CAGCTGGTCC 
CTGCTAGAGC AGCTGGGCCT GGCCGGGGCA GACCTGGCGG CCCCCGGGGT ACAGCAGCAG 
CTGGAGCTGG AGCGGGAGCG GCTGCGGCGG GAAATCCGCA AGGAGCTGAA GCTGAAGGAG 
GGTGCTGAGA ACCTGCGGCG GGCCACCACT GACCTGGGCC GCAGCCTGGG CCCCGTAGAG 
CTGCTGCTGC GGGGCTCCTC GCGCCGCCTC GACCTGCTGC ACCAGCAGCT GCAGGAGCTG 
CACGCCCACG TGGTGCTTCC CGACCCGGCG GCCACCCACG ATGGCCCCCA GTCCCCTGGT 
GCGGGTGGCC CCACCTGCTC GGCCACCAAC CTGAGCCGCG TGGCGGGCCT GGAGAAGCAG 
TTGGCCATTG AGCTGAAGGT GAAGCAGGGG GCGGAGAACA TGATCCAGAC CTACAGCAAT 
GGCAGCACCA AGGACCGGAA GCTGCTGCTG ACAGCCCAGC AGATGTTGCA GGACAGTAAG 
ACCAAGATTG ACATCATCCG CATGCAACTC CGCCGGGCGC TGCAGGCCGG CCAGCTGGAG 
AACCAGGCAG CCCCGGATGA CACCCAAGGG AGTCCTGACC TGGGGGCTGT GGAGCTGCGC 
ATCGAAGAGC TGCGGCACCA CTTCCGAGTG GAGCACGCGG TGGCCGAGGG TGCCAAGAAC 
GTACTGCGCC TGCTCAGCGC TGCCAAGGCC CCGGACCGCA AGGCAGTCAG CGAGGCCCAG 
GAGAAATTAA CAGAATCCAA CCAGAAGCTG GGGCTGCTGC GGGAGGCTCT GGAGCGGAGA 
CTTGGGGAGC TGCCCGCCGA CCACCCCAAG GGGCGGCTGC TGCGAGAAGA GCTCGCTGCG 
GCCTCCTCCG CTGCCTTCAG CACCCGCCTG GCCGGGCCCT TTCCCGCCAC GCACTACAGC 
ACCCTGTGCA AGCCCGCGCC GCTCACAGGG ACCCTGGAGG TACGAGTGGT GGGCTGCAGA 
GACCTCCCAG AGACCATCCC GTGGAACCCT ACCCCCTCAA TGGGGGGACC TGGGACCCCA 
GACAGCCGCC CCCCCTTCCT GAGCCGCCCA GCCCGGGGCC TTTACAGCCG AAGCGGAAGC 
CTCAGTGGCC GGAGCAGCCT CAAAGCAGAA GCCGAGAACA CCAGTGAAGT CAGCACTGTG 
CTTAAGCTGG ATAACACAGT GGTGGGGCAG ACGTCTTGGA AGCCATGTGG CCCCAATGCC 
TGGGACCAGA GCTTCACTCT GGAGCTGGAA AGGGCACGGG AACTGGAGTT GGCTGTGTTC 
TGGCGGGACC AGCGGGGCCT GTGTGCCCTC AAATTCCTGA AGTTGGAGGA TTTCTTGGAC 
AATGAGAGGC ATGAGGTGCA GCTGGACATG GAACCCCAGG GCTGCCTGGT GGCTGAGGTC 
ACCTTCCGCA ACCCTGTCAT TGAGAGGATT CCTCGGCTCC GACGGCAGAA GAAAATTTTC 
TCCAAGCAGC AAGGGAAGGC GTTCCAGCGT GCTAGGCAGA TGAACATCGA TGTCGCCACG 
TGGGTGCGGC TGCTCCGGAG GCTCATCCCC AATGCCACGG GCACAGGCAC CTTTAGCCCT 
GGGGCTTCTC CAGGATCCGA GGCCCGGACC ACGGGTGACA TATCGGTGGA GAAGCTGAAC 
CTCGGCACTG ACTCGGACAG CTCACCTCAG AAGAGCTCGC GGGATCCTCC TTCCAGCCCA 
TCGAGCCTGA GCTCCCCCAT CCAGGAATCC ACTGCTCCCG AGCTGCCTTC GGAGACCCAG 
GAGACCCCAG GCCCCGCCCT GTGCAGCCCT CTGAGGAAGT CACCTCTGAC CCTCGAAGAT 
TTCAAGTTCC TGGCGGTGCT GGGCCGGGGT CATTTTGGGA AGGTGCTCCT CTCCGAATTC 
CGGCCCAGTG GGGAGCTGTT CGCCATCAAG GCTCTGAAGA AAGGGGACAT TGTGGCCCGA 
GACGAGGTGG AGAGCCTGAT GTGTGAGAAG CGGATATTGG CGGCAGTGAC CAGTGCGGGA 
CACCCCTTCC TGGTGAACCT CTTCGGCTGT TTCCAGACAC CGGAGCACGT GTGCTTCGTG 
ATGGAGTACT CGGCCGGTGG GGACCTGATG CTGCACATCC ACAGCGACGT GTTCTCTGAG 
CCCCGTGCCA TCTTTTATTC CGCCTGCGTG GTGCTGGGCC TACAGTTTCT TCACGAACAC 
AAGATCGTCT ACAGGGACCT GAAGTTGGAC AATTTGCTCC TGGACACCGA GGGCTACGTC 
AAGATCGCAG ACTTTGGCCT CTGCAAGGAG GGGATGGGCT ATGGGGACCG GACCAGCACA 
TTCTGTGGGA CCCCGGAGTT CCTGGCCCCT GAGGTGCTGA CGGACACGTC GTACACGCGA 
GCTGTGGACT GGTGGGGACT GGGTGTGCTG CTCTACGAGA TGCTGGTTGG CGAGTCCCCA 
TTCCCAGGGG ATGATGAGGA GGAGGTCTTC GACAGCATCG TCAACGACGA GGTTCGCTAC 
CCCCGCTTCC TGTCGGCCGA AGCCATCGGC ATCATGAGAA GGCTGCTTCG GAGGAACCCA 
GAGCGGAGGC TGGGATCTAG CGAGAGAGAT GCAGAAGATG TGAAGAAACA GCCCTTCTTC 
AGGACTCTGG GCTGGGAAGC CCTGTTGGCC CGGCGCCTGC CACCGCCCTT TGTGCCCACG 
CTGTCCGGCC GCACCGACGT CAGCAACTTC GACGAGGAGT TCACCGGGGA GGCCCCCACA 
CTGAGCCCGC CCCGCGACGC GCGGCCCCTC ACAGCCGCGG AGCAGGCAGC CTTCCTGGAC 
TTCGACTTCG TGGCCGGGGG CTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PKN1 ( NM_002741.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_002732.3 Homo sapiens protein kinase N1 (PKN1), transcript variant 2, mRNA.

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CloneID	OHu107140
Clone ID Related Accession (Same CDS sequence)	NM_002741.5 , NM_002741.4
Accession Version	NM_002741.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-03
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase N1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008569.7. On Jun 2, 2019 this sequence version replaced NM_002741.4. Transcript Variant: This variant (2) uses an alternate exon at the 5' end compared to variant 1, which includes a part of the coding region. The resulting isoform (2) has a distinct and shorter N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040061.1, S75546.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGCG ACGCCGTGCA GAGTGAGCCT CGCAGCTGGT CCCTGCTAGA GCAGCTGGGC 
CTGGCCGGGG CAGACCTGGC GGCCCCCGGG GTACAGCAGC AGCTGGAGCT GGAGCGGGAG 
CGGCTGCGGC GGGAAATCCG CAAGGAGCTG AAGCTGAAGG AGGGTGCTGA GAACCTGCGG 
CGGGCCACCA CTGACCTGGG CCGCAGCCTG GGCCCCGTAG AGCTGCTGCT GCGGGGCTCC 
TCGCGCCGCC TCGACCTGCT GCACCAGCAG CTGCAGGAGC TGCACGCCCA CGTGGTGCTT 
CCCGACCCGG CGGCCACCCA CGATGGCCCC CAGTCCCCTG GTGCGGGTGG CCCCACCTGC 
TCGGCCACCA ACCTGAGCCG CGTGGCGGGC CTGGAGAAGC AGTTGGCCAT TGAGCTGAAG 
GTGAAGCAGG GGGCGGAGAA CATGATCCAG ACCTACAGCA ATGGCAGCAC CAAGGACCGG 
AAGCTGCTGC TGACAGCCCA GCAGATGTTG CAGGACAGTA AGACCAAGAT TGACATCATC 
CGCATGCAAC TCCGCCGGGC GCTGCAGGCC GGCCAGCTGG AGAACCAGGC AGCCCCGGAT 
GACACCCAAG GGAGTCCTGA CCTGGGGGCT GTGGAGCTGC GCATCGAAGA GCTGCGGCAC 
CACTTCCGAG TGGAGCACGC GGTGGCCGAG GGTGCCAAGA ACGTACTGCG CCTGCTCAGC 
GCTGCCAAGG CCCCGGACCG CAAGGCAGTC AGCGAGGCCC AGGAGAAATT AACAGAATCC 
AACCAGAAGC TGGGGCTGCT GCGGGAGGCT CTGGAGCGGA GACTTGGGGA GCTGCCCGCC 
GACCACCCCA AGGGGCGGCT GCTGCGAGAA GAGCTCGCTG CGGCCTCCTC CGCTGCCTTC 
AGCACCCGCC TGGCCGGGCC CTTTCCCGCC ACGCACTACA GCACCCTGTG CAAGCCCGCG 
CCGCTCACAG GGACCCTGGA GGTACGAGTG GTGGGCTGCA GAGACCTCCC AGAGACCATC 
CCGTGGAACC CTACCCCCTC AATGGGGGGA CCTGGGACCC CAGACAGCCG CCCCCCCTTC 
CTGAGCCGCC CAGCCCGGGG CCTTTACAGC CGAAGCGGAA GCCTCAGTGG CCGGAGCAGC 
CTCAAAGCAG AAGCCGAGAA CACCAGTGAA GTCAGCACTG TGCTTAAGCT GGATAACACA 
GTGGTGGGGC AGACGTCTTG GAAGCCATGT GGCCCCAATG CCTGGGACCA GAGCTTCACT 
CTGGAGCTGG AAAGGGCACG GGAACTGGAG TTGGCTGTGT TCTGGCGGGA CCAGCGGGGC 
CTGTGTGCCC TCAAATTCCT GAAGTTGGAG GATTTCTTGG ACAATGAGAG GCATGAGGTG 
CAGCTGGACA TGGAACCCCA GGGCTGCCTG GTGGCTGAGG TCACCTTCCG CAACCCTGTC 
ATTGAGAGGA TTCCTCGGCT CCGACGGCAG AAGAAAATTT TCTCCAAGCA GCAAGGGAAG 
GCGTTCCAGC GTGCTAGGCA GATGAACATC GATGTCGCCA CGTGGGTGCG GCTGCTCCGG 
AGGCTCATCC CCAATGCCAC GGGCACAGGC ACCTTTAGCC CTGGGGCTTC TCCAGGATCC 
GAGGCCCGGA CCACGGGTGA CATATCGGTG GAGAAGCTGA ACCTCGGCAC TGACTCGGAC 
AGCTCACCTC AGAAGAGCTC GCGGGATCCT CCTTCCAGCC CATCGAGCCT GAGCTCCCCC 
ATCCAGGAAT CCACTGCTCC CGAGCTGCCT TCGGAGACCC AGGAGACCCC AGGCCCCGCC 
CTGTGCAGCC CTCTGAGGAA GTCACCTCTG ACCCTCGAAG ATTTCAAGTT CCTGGCGGTG 
CTGGGCCGGG GTCATTTTGG GAAGGTGCTC CTCTCCGAAT TCCGGCCCAG TGGGGAGCTG 
TTCGCCATCA AGGCTCTGAA GAAAGGGGAC ATTGTGGCCC GAGACGAGGT GGAGAGCCTG 
ATGTGTGAGA AGCGGATATT GGCGGCAGTG ACCAGTGCGG GACACCCCTT CCTGGTGAAC 
CTCTTCGGCT GTTTCCAGAC ACCGGAGCAC GTGTGCTTCG TGATGGAGTA CTCGGCCGGT 
GGGGACCTGA TGCTGCACAT CCACAGCGAC GTGTTCTCTG AGCCCCGTGC CATCTTTTAT 
TCCGCCTGCG TGGTGCTGGG CCTACAGTTT CTTCACGAAC ACAAGATCGT CTACAGGGAC 
CTGAAGTTGG ACAATTTGCT CCTGGACACC GAGGGCTACG TCAAGATCGC AGACTTTGGC 
CTCTGCAAGG AGGGGATGGG CTATGGGGAC CGGACCAGCA CATTCTGTGG GACCCCGGAG 
TTCCTGGCCC CTGAGGTGCT GACGGACACG TCGTACACGC GAGCTGTGGA CTGGTGGGGA 
CTGGGTGTGC TGCTCTACGA GATGCTGGTT GGCGAGTCCC CATTCCCAGG GGATGATGAG 
GAGGAGGTCT TCGACAGCAT CGTCAACGAC GAGGTTCGCT ACCCCCGCTT CCTGTCGGCC 
GAAGCCATCG GCATCATGAG AAGGCTGCTT CGGAGGAACC CAGAGCGGAG GCTGGGATCT 
AGCGAGAGAG ATGCAGAAGA TGTGAAGAAA CAGCCCTTCT TCAGGACTCT GGGCTGGGAA 
GCCCTGTTGG CCCGGCGCCT GCCACCGCCC TTTGTGCCCA CGCTGTCCGG CCGCACCGAC 
GTCAGCAACT TCGACGAGGA GTTCACCGGG GAGGCCCCCA CACTGAGCCC GCCCCGCGAC 
GCGCGGCCCC TCACAGCCGC GGAGCAGGCA GCCTTCCTGG ACTTCGACTT CGTGGCCGGG 
GGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002732.3
CDS	217..3045
Translation	MASDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC

Target ORF information:

RefSeq Version	NM_002741.5
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase N1 (PKN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002741.5

ORF Insert Sequence:

ATGGCCAGCG ACGCCGTGCA GAGTGAGCCT CGCAGCTGGT CCCTGCTAGA GCAGCTGGGC 
CTGGCCGGGG CAGACCTGGC GGCCCCCGGG GTACAGCAGC AGCTGGAGCT GGAGCGGGAG 
CGGCTGCGGC GGGAAATCCG CAAGGAGCTG AAGCTGAAGG AGGGTGCTGA GAACCTGCGG 
CGGGCCACCA CTGACCTGGG CCGCAGCCTG GGCCCCGTAG AGCTGCTGCT GCGGGGCTCC 
TCGCGCCGCC TCGACCTGCT GCACCAGCAG CTGCAGGAGC TGCACGCCCA CGTGGTGCTT 
CCCGACCCGG CGGCCACCCA CGATGGCCCC CAGTCCCCTG GTGCGGGTGG CCCCACCTGC 
TCGGCCACCA ACCTGAGCCG CGTGGCGGGC CTGGAGAAGC AGTTGGCCAT TGAGCTGAAG 
GTGAAGCAGG GGGCGGAGAA CATGATCCAG ACCTACAGCA ATGGCAGCAC CAAGGACCGG 
AAGCTGCTGC TGACAGCCCA GCAGATGTTG CAGGACAGTA AGACCAAGAT TGACATCATC 
CGCATGCAAC TCCGCCGGGC GCTGCAGGCC GGCCAGCTGG AGAACCAGGC AGCCCCGGAT 
GACACCCAAG GGAGTCCTGA CCTGGGGGCT GTGGAGCTGC GCATCGAAGA GCTGCGGCAC 
CACTTCCGAG TGGAGCACGC GGTGGCCGAG GGTGCCAAGA ACGTACTGCG CCTGCTCAGC 
GCTGCCAAGG CCCCGGACCG CAAGGCAGTC AGCGAGGCCC AGGAGAAATT AACAGAATCC 
AACCAGAAGC TGGGGCTGCT GCGGGAGGCT CTGGAGCGGA GACTTGGGGA GCTGCCCGCC 
GACCACCCCA AGGGGCGGCT GCTGCGAGAA GAGCTCGCTG CGGCCTCCTC CGCTGCCTTC 
AGCACCCGCC TGGCCGGGCC CTTTCCCGCC ACGCACTACA GCACCCTGTG CAAGCCCGCG 
CCGCTCACAG GGACCCTGGA GGTACGAGTG GTGGGCTGCA GAGACCTCCC AGAGACCATC 
CCGTGGAACC CTACCCCCTC AATGGGGGGA CCTGGGACCC CAGACAGCCG CCCCCCCTTC 
CTGAGCCGCC CAGCCCGGGG CCTTTACAGC CGAAGCGGAA GCCTCAGTGG CCGGAGCAGC 
CTCAAAGCAG AAGCCGAGAA CACCAGTGAA GTCAGCACTG TGCTTAAGCT GGATAACACA 
GTGGTGGGGC AGACGTCTTG GAAGCCATGT GGCCCCAATG CCTGGGACCA GAGCTTCACT 
CTGGAGCTGG AAAGGGCACG GGAACTGGAG TTGGCTGTGT TCTGGCGGGA CCAGCGGGGC 
CTGTGTGCCC TCAAATTCCT GAAGTTGGAG GATTTCTTGG ACAATGAGAG GCATGAGGTG 
CAGCTGGACA TGGAACCCCA GGGCTGCCTG GTGGCTGAGG TCACCTTCCG CAACCCTGTC 
ATTGAGAGGA TTCCTCGGCT CCGACGGCAG AAGAAAATTT TCTCCAAGCA GCAAGGGAAG 
GCGTTCCAGC GTGCTAGGCA GATGAACATC GATGTCGCCA CGTGGGTGCG GCTGCTCCGG 
AGGCTCATCC CCAATGCCAC GGGCACAGGC ACCTTTAGCC CTGGGGCTTC TCCAGGATCC 
GAGGCCCGGA CCACGGGTGA CATATCGGTG GAGAAGCTGA ACCTCGGCAC TGACTCGGAC 
AGCTCACCTC AGAAGAGCTC GCGGGATCCT CCTTCCAGCC CATCGAGCCT GAGCTCCCCC 
ATCCAGGAAT CCACTGCTCC CGAGCTGCCT TCGGAGACCC AGGAGACCCC AGGCCCCGCC 
CTGTGCAGCC CTCTGAGGAA GTCACCTCTG ACCCTCGAAG ATTTCAAGTT CCTGGCGGTG 
CTGGGCCGGG GTCATTTTGG GAAGGTGCTC CTCTCCGAAT TCCGGCCCAG TGGGGAGCTG 
TTCGCCATCA AGGCTCTGAA GAAAGGGGAC ATTGTGGCCC GAGACGAGGT GGAGAGCCTG 
ATGTGTGAGA AGCGGATATT GGCGGCAGTG ACCAGTGCGG GACACCCCTT CCTGGTGAAC 
CTCTTCGGCT GTTTCCAGAC ACCGGAGCAC GTGTGCTTCG TGATGGAGTA CTCGGCCGGT 
GGGGACCTGA TGCTGCACAT CCACAGCGAC GTGTTCTCTG AGCCCCGTGC CATCTTTTAT 
TCCGCCTGCG TGGTGCTGGG CCTACAGTTT CTTCACGAAC ACAAGATCGT CTACAGGGAC 
CTGAAGTTGG ACAATTTGCT CCTGGACACC GAGGGCTACG TCAAGATCGC AGACTTTGGC 
CTCTGCAAGG AGGGGATGGG CTATGGGGAC CGGACCAGCA CATTCTGTGG GACCCCGGAG 
TTCCTGGCCC CTGAGGTGCT GACGGACACG TCGTACACGC GAGCTGTGGA CTGGTGGGGA 
CTGGGTGTGC TGCTCTACGA GATGCTGGTT GGCGAGTCCC CATTCCCAGG GGATGATGAG 
GAGGAGGTCT TCGACAGCAT CGTCAACGAC GAGGTTCGCT ACCCCCGCTT CCTGTCGGCC 
GAAGCCATCG GCATCATGAG AAGGCTGCTT CGGAGGAACC CAGAGCGGAG GCTGGGATCT 
AGCGAGAGAG ATGCAGAAGA TGTGAAGAAA CAGCCCTTCT TCAGGACTCT GGGCTGGGAA 
GCCCTGTTGG CCCGGCGCCT GCCACCGCCC TTTGTGCCCA CGCTGTCCGG CCGCACCGAC 
GTCAGCAACT TCGACGAGGA GTTCACCGGG GAGGCCCCCA CACTGAGCCC GCCCCGCGAC 
GCGCGGCCCC TCACAGCCGC GGAGCAGGCA GCCTTCCTGG ACTTCGACTT CGTGGCCGGG 
GGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PKN1 ( NM_002741.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_002732.3 Homo sapiens protein kinase N1 (PKN1), transcript variant 2, mRNA.

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CloneID	OHu107140
Clone ID Related Accession (Same CDS sequence)	NM_002741.5 , NM_002741.4
Accession Version	NM_002741.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2829bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-09
Organism	Homo sapiens(human)
Product	serine/threonine-protein kinase N1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC008569.7. On Aug 25, 2018 this sequence version replaced NM_002741.3. Transcript Variant: This variant (2) uses an alternate exon at the 5' end compared to variant 1, which includes a part of the coding region. The resulting isoform (2) has a distinct and shorter N-terminus, as compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC040061.1, S75546.1 [ECO:0000332] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCCAGCG ACGCCGTGCA GAGTGAGCCT CGCAGCTGGT CCCTGCTAGA GCAGCTGGGC 
CTGGCCGGGG CAGACCTGGC GGCCCCCGGG GTACAGCAGC AGCTGGAGCT GGAGCGGGAG 
CGGCTGCGGC GGGAAATCCG CAAGGAGCTG AAGCTGAAGG AGGGTGCTGA GAACCTGCGG 
CGGGCCACCA CTGACCTGGG CCGCAGCCTG GGCCCCGTAG AGCTGCTGCT GCGGGGCTCC 
TCGCGCCGCC TCGACCTGCT GCACCAGCAG CTGCAGGAGC TGCACGCCCA CGTGGTGCTT 
CCCGACCCGG CGGCCACCCA CGATGGCCCC CAGTCCCCTG GTGCGGGTGG CCCCACCTGC 
TCGGCCACCA ACCTGAGCCG CGTGGCGGGC CTGGAGAAGC AGTTGGCCAT TGAGCTGAAG 
GTGAAGCAGG GGGCGGAGAA CATGATCCAG ACCTACAGCA ATGGCAGCAC CAAGGACCGG 
AAGCTGCTGC TGACAGCCCA GCAGATGTTG CAGGACAGTA AGACCAAGAT TGACATCATC 
CGCATGCAAC TCCGCCGGGC GCTGCAGGCC GGCCAGCTGG AGAACCAGGC AGCCCCGGAT 
GACACCCAAG GGAGTCCTGA CCTGGGGGCT GTGGAGCTGC GCATCGAAGA GCTGCGGCAC 
CACTTCCGAG TGGAGCACGC GGTGGCCGAG GGTGCCAAGA ACGTACTGCG CCTGCTCAGC 
GCTGCCAAGG CCCCGGACCG CAAGGCAGTC AGCGAGGCCC AGGAGAAATT AACAGAATCC 
AACCAGAAGC TGGGGCTGCT GCGGGAGGCT CTGGAGCGGA GACTTGGGGA GCTGCCCGCC 
GACCACCCCA AGGGGCGGCT GCTGCGAGAA GAGCTCGCTG CGGCCTCCTC CGCTGCCTTC 
AGCACCCGCC TGGCCGGGCC CTTTCCCGCC ACGCACTACA GCACCCTGTG CAAGCCCGCG 
CCGCTCACAG GGACCCTGGA GGTACGAGTG GTGGGCTGCA GAGACCTCCC AGAGACCATC 
CCGTGGAACC CTACCCCCTC AATGGGGGGA CCTGGGACCC CAGACAGCCG CCCCCCCTTC 
CTGAGCCGCC CAGCCCGGGG CCTTTACAGC CGAAGCGGAA GCCTCAGTGG CCGGAGCAGC 
CTCAAAGCAG AAGCCGAGAA CACCAGTGAA GTCAGCACTG TGCTTAAGCT GGATAACACA 
GTGGTGGGGC AGACGTCTTG GAAGCCATGT GGCCCCAATG CCTGGGACCA GAGCTTCACT 
CTGGAGCTGG AAAGGGCACG GGAACTGGAG TTGGCTGTGT TCTGGCGGGA CCAGCGGGGC 
CTGTGTGCCC TCAAATTCCT GAAGTTGGAG GATTTCTTGG ACAATGAGAG GCATGAGGTG 
CAGCTGGACA TGGAACCCCA GGGCTGCCTG GTGGCTGAGG TCACCTTCCG CAACCCTGTC 
ATTGAGAGGA TTCCTCGGCT CCGACGGCAG AAGAAAATTT TCTCCAAGCA GCAAGGGAAG 
GCGTTCCAGC GTGCTAGGCA GATGAACATC GATGTCGCCA CGTGGGTGCG GCTGCTCCGG 
AGGCTCATCC CCAATGCCAC GGGCACAGGC ACCTTTAGCC CTGGGGCTTC TCCAGGATCC 
GAGGCCCGGA CCACGGGTGA CATATCGGTG GAGAAGCTGA ACCTCGGCAC TGACTCGGAC 
AGCTCACCTC AGAAGAGCTC GCGGGATCCT CCTTCCAGCC CATCGAGCCT GAGCTCCCCC 
ATCCAGGAAT CCACTGCTCC CGAGCTGCCT TCGGAGACCC AGGAGACCCC AGGCCCCGCC 
CTGTGCAGCC CTCTGAGGAA GTCACCTCTG ACCCTCGAAG ATTTCAAGTT CCTGGCGGTG 
CTGGGCCGGG GTCATTTTGG GAAGGTGCTC CTCTCCGAAT TCCGGCCCAG TGGGGAGCTG 
TTCGCCATCA AGGCTCTGAA GAAAGGGGAC ATTGTGGCCC GAGACGAGGT GGAGAGCCTG 
ATGTGTGAGA AGCGGATATT GGCGGCAGTG ACCAGTGCGG GACACCCCTT CCTGGTGAAC 
CTCTTCGGCT GTTTCCAGAC ACCGGAGCAC GTGTGCTTCG TGATGGAGTA CTCGGCCGGT 
GGGGACCTGA TGCTGCACAT CCACAGCGAC GTGTTCTCTG AGCCCCGTGC CATCTTTTAT 
TCCGCCTGCG TGGTGCTGGG CCTACAGTTT CTTCACGAAC ACAAGATCGT CTACAGGGAC 
CTGAAGTTGG ACAATTTGCT CCTGGACACC GAGGGCTACG TCAAGATCGC AGACTTTGGC 
CTCTGCAAGG AGGGGATGGG CTATGGGGAC CGGACCAGCA CATTCTGTGG GACCCCGGAG 
TTCCTGGCCC CTGAGGTGCT GACGGACACG TCGTACACGC GAGCTGTGGA CTGGTGGGGA 
CTGGGTGTGC TGCTCTACGA GATGCTGGTT GGCGAGTCCC CATTCCCAGG GGATGATGAG 
GAGGAGGTCT TCGACAGCAT CGTCAACGAC GAGGTTCGCT ACCCCCGCTT CCTGTCGGCC 
GAAGCCATCG GCATCATGAG AAGGCTGCTT CGGAGGAACC CAGAGCGGAG GCTGGGATCT 
AGCGAGAGAG ATGCAGAAGA TGTGAAGAAA CAGCCCTTCT TCAGGACTCT GGGCTGGGAA 
GCCCTGTTGG CCCGGCGCCT GCCACCGCCC TTTGTGCCCA CGCTGTCCGG CCGCACCGAC 
GTCAGCAACT TCGACGAGGA GTTCACCGGG GAGGCCCCCA CACTGAGCCC GCCCCGCGAC 
GCGCGGCCCC TCACAGCCGC GGAGCAGGCA GCCTTCCTGG ACTTCGACTT CGTGGCCGGG 
GGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_002732.3
CDS	217..3045
Translation	MASDAVQSEPRSWSLLEQLGLAGADLAAPGVQQQLELERERLRREIRKELKLKEGAENLRRATTDLGRSLGPVELLLRGSSRRLDLLHQQLQELHAHVVLPDPAATHDGPQSPGAGGPTCSATNLSRVAGLEKQLAIELKVKQGAENMIQTYSNGSTKDRKLLLTAQQMLQDSKTKIDIIRMQLRRALQAGQLENQAAPDDTQGSPDLGAVELRIEELRHHFRVEHAVAEGAKNVLRLLSAAKAPDRKAVSEAQEKLTESNQKLGLLREALERRLGELPADHPKGRLLREELAAASSAAFSTRLAGPFPATHYSTLCKPAPLTGTLEVRVVGCRDLPETIPWNPTPSMGGPGTPDSRPPFLSRPARGLYSRSGSLSGRSSLKAEAENTSEVSTVLKLDNTVVGQTSWKPCGPNAWDQSFTLELERARELELAVFWRDQRGLCALKFLKLEDFLDNERHEVQLDMEPQGCLVAEVTFRNPVIERIPRLRRQKKIFSKQQGKAFQRARQMNIDVATWVRLLRRLIPNATGTGTFSPGASPGSEARTTGDISVEKLNLGTDSDSSPQKSSRDPPSSPSSLSSPIQESTAPELPSETQETPGPALCSPLRKSPLTLEDFKFLAVLGRGHFGKVLLSEFRPSGELFAIKALKKGDIVARDEVESLMCEKRILAAVTSAGHPFLVNLFGCFQTPEHVCFVMEYSAGGDLMLHIHSDVFSEPRAIFYSACVVLGLQFLHEHKIVYRDLKLDNLLLDTEGYVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTDTSYTRAVDWWGLGVLLYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSAEAIGIMRRLLRRNPERRLGSSERDAEDVKKQPFFRTLGWEALLARRLPPPFVPTLSGRTDVSNFDEEFTGEAPTLSPPRDARPLTAAEQAAFLDFDFVAGGC

Target ORF information:

RefSeq Version	NM_002741.4
Organism	Homo sapiens(human)
Definition	Homo sapiens protein kinase N1 (PKN1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_002741.4

ORF Insert Sequence:

ATGGCCAGCG ACGCCGTGCA GAGTGAGCCT CGCAGCTGGT CCCTGCTAGA GCAGCTGGGC 
CTGGCCGGGG CAGACCTGGC GGCCCCCGGG GTACAGCAGC AGCTGGAGCT GGAGCGGGAG 
CGGCTGCGGC GGGAAATCCG CAAGGAGCTG AAGCTGAAGG AGGGTGCTGA GAACCTGCGG 
CGGGCCACCA CTGACCTGGG CCGCAGCCTG GGCCCCGTAG AGCTGCTGCT GCGGGGCTCC 
TCGCGCCGCC TCGACCTGCT GCACCAGCAG CTGCAGGAGC TGCACGCCCA CGTGGTGCTT 
CCCGACCCGG CGGCCACCCA CGATGGCCCC CAGTCCCCTG GTGCGGGTGG CCCCACCTGC 
TCGGCCACCA ACCTGAGCCG CGTGGCGGGC CTGGAGAAGC AGTTGGCCAT TGAGCTGAAG 
GTGAAGCAGG GGGCGGAGAA CATGATCCAG ACCTACAGCA ATGGCAGCAC CAAGGACCGG 
AAGCTGCTGC TGACAGCCCA GCAGATGTTG CAGGACAGTA AGACCAAGAT TGACATCATC 
CGCATGCAAC TCCGCCGGGC GCTGCAGGCC GGCCAGCTGG AGAACCAGGC AGCCCCGGAT 
GACACCCAAG GGAGTCCTGA CCTGGGGGCT GTGGAGCTGC GCATCGAAGA GCTGCGGCAC 
CACTTCCGAG TGGAGCACGC GGTGGCCGAG GGTGCCAAGA ACGTACTGCG CCTGCTCAGC 
GCTGCCAAGG CCCCGGACCG CAAGGCAGTC AGCGAGGCCC AGGAGAAATT AACAGAATCC 
AACCAGAAGC TGGGGCTGCT GCGGGAGGCT CTGGAGCGGA GACTTGGGGA GCTGCCCGCC 
GACCACCCCA AGGGGCGGCT GCTGCGAGAA GAGCTCGCTG CGGCCTCCTC CGCTGCCTTC 
AGCACCCGCC TGGCCGGGCC CTTTCCCGCC ACGCACTACA GCACCCTGTG CAAGCCCGCG 
CCGCTCACAG GGACCCTGGA GGTACGAGTG GTGGGCTGCA GAGACCTCCC AGAGACCATC 
CCGTGGAACC CTACCCCCTC AATGGGGGGA CCTGGGACCC CAGACAGCCG CCCCCCCTTC 
CTGAGCCGCC CAGCCCGGGG CCTTTACAGC CGAAGCGGAA GCCTCAGTGG CCGGAGCAGC 
CTCAAAGCAG AAGCCGAGAA CACCAGTGAA GTCAGCACTG TGCTTAAGCT GGATAACACA 
GTGGTGGGGC AGACGTCTTG GAAGCCATGT GGCCCCAATG CCTGGGACCA GAGCTTCACT 
CTGGAGCTGG AAAGGGCACG GGAACTGGAG TTGGCTGTGT TCTGGCGGGA CCAGCGGGGC 
CTGTGTGCCC TCAAATTCCT GAAGTTGGAG GATTTCTTGG ACAATGAGAG GCATGAGGTG 
CAGCTGGACA TGGAACCCCA GGGCTGCCTG GTGGCTGAGG TCACCTTCCG CAACCCTGTC 
ATTGAGAGGA TTCCTCGGCT CCGACGGCAG AAGAAAATTT TCTCCAAGCA GCAAGGGAAG 
GCGTTCCAGC GTGCTAGGCA GATGAACATC GATGTCGCCA CGTGGGTGCG GCTGCTCCGG 
AGGCTCATCC CCAATGCCAC GGGCACAGGC ACCTTTAGCC CTGGGGCTTC TCCAGGATCC 
GAGGCCCGGA CCACGGGTGA CATATCGGTG GAGAAGCTGA ACCTCGGCAC TGACTCGGAC 
AGCTCACCTC AGAAGAGCTC GCGGGATCCT CCTTCCAGCC CATCGAGCCT GAGCTCCCCC 
ATCCAGGAAT CCACTGCTCC CGAGCTGCCT TCGGAGACCC AGGAGACCCC AGGCCCCGCC 
CTGTGCAGCC CTCTGAGGAA GTCACCTCTG ACCCTCGAAG ATTTCAAGTT CCTGGCGGTG 
CTGGGCCGGG GTCATTTTGG GAAGGTGCTC CTCTCCGAAT TCCGGCCCAG TGGGGAGCTG 
TTCGCCATCA AGGCTCTGAA GAAAGGGGAC ATTGTGGCCC GAGACGAGGT GGAGAGCCTG 
ATGTGTGAGA AGCGGATATT GGCGGCAGTG ACCAGTGCGG GACACCCCTT CCTGGTGAAC 
CTCTTCGGCT GTTTCCAGAC ACCGGAGCAC GTGTGCTTCG TGATGGAGTA CTCGGCCGGT 
GGGGACCTGA TGCTGCACAT CCACAGCGAC GTGTTCTCTG AGCCCCGTGC CATCTTTTAT 
TCCGCCTGCG TGGTGCTGGG CCTACAGTTT CTTCACGAAC ACAAGATCGT CTACAGGGAC 
CTGAAGTTGG ACAATTTGCT CCTGGACACC GAGGGCTACG TCAAGATCGC AGACTTTGGC 
CTCTGCAAGG AGGGGATGGG CTATGGGGAC CGGACCAGCA CATTCTGTGG GACCCCGGAG 
TTCCTGGCCC CTGAGGTGCT GACGGACACG TCGTACACGC GAGCTGTGGA CTGGTGGGGA 
CTGGGTGTGC TGCTCTACGA GATGCTGGTT GGCGAGTCCC CATTCCCAGG GGATGATGAG 
GAGGAGGTCT TCGACAGCAT CGTCAACGAC GAGGTTCGCT ACCCCCGCTT CCTGTCGGCC 
GAAGCCATCG GCATCATGAG AAGGCTGCTT CGGAGGAACC CAGAGCGGAG GCTGGGATCT 
AGCGAGAGAG ATGCAGAAGA TGTGAAGAAA CAGCCCTTCT TCAGGACTCT GGGCTGGGAA 
GCCCTGTTGG CCCGGCGCCT GCCACCGCCC TTTGTGCCCA CGCTGTCCGG CCGCACCGAC 
GTCAGCAACT TCGACGAGGA GTTCACCGGG GAGGCCCCCA CACTGAGCCC GCCCCGCGAC 
GCGCGGCCCC TCACAGCCGC GGAGCAGGCA GCCTTCCTGG ACTTCGACTT CGTGGCCGGG 
GGCTGCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The protein kinase C super-family member PKN is regulated by mTOR and influences differentiation during prostate cancer progression.
The Prostate77(15)1452-1467(2017 Nov)
Yang CS,Melhuish TA,Spencer A,Ni L,Hao Y,Jividen K,Harris TE,Snow C,Frierson HF Jr,Wotton D,Paschal BM

RhoA promotes epidermal stem cell proliferation via PKN1-cyclin D1 signaling.
PloS one12(2)e0172613(2017)
Wang F,Zhan R,Chen L,Dai X,Wang W,Guo R,Li X,Li Z,Wang L,Huang S,Shen J,Li S,Cao C

Protein kinase C-related kinase 1 and 2 play an essential role in thromboxane-mediated neoplastic responses in prostate cancer.
Oncotarget6(28)26437-56(2015 Sep)
O'Sullivan AG,Mulvaney EP,Hyland PB,Kinsella BT

PRK1/PKN1 controls migration and metastasis of androgen-independent prostate cancer cells.
Oncotarget5(24)12646-64(2014 Dec)
Jilg CA,Ketscher A,Metzger E,Hummel B,Willmann D,R?sseler V,Drendel V,Imhof A,Jung M,Franz H,H?lz S,Kr?nig M,M?ller JM,Sch?le R

Enzyme kinetics and distinct modulation of the protein kinase N family of kinases by lipid activators and small molecule inhibitors.
Bioscience reports34(2)(2014 Apr)
Falk MD,Liu W,Bola?os B,Unsal-Kacmaz K,Klippel A,Grant S,Brooun A,Timofeevski S

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PKN1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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