CAND1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	CAND1
Entrez Gene ID	55832
Full Name	cullin associated and neddylation dissociated 1
Synonyms	TIP120,TIP120A
General protein information	Preferred Names cullin associated and neddylation dissociated 1 Names cullin-associated NEDD8-dissociated protein 1 TBP-interacting protein of 120 kDa A p120 CAND1
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 12 Map Location: 12q14.3-q15
Summary	This gene encodes an essential regulator of Cullin-RING ubiquitin ligases, which are in involved in ubiquitinylation of proteins degraded by the Ub proteasome system. The encoded protein binds to unneddylated cullin-RING box protein complexes and acts as an inhibitor of cullin neddylation and of Skp1, cullin, and F box ubiquitin ligase complex assembly and activity. In mammalian cell culture, this protein predominantly localizes to the cytoplasm. Knockdown of this gene in preadipocytes results in blocked adipogenesis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2016].
Disorder MIM:	607727

mRNA and Protein(s)

mRNA	Protein	Name
NM_001329674.1	NP_001316603.1	cullin-associated NEDD8-dissociated protein 1 isoform 2
NM_001329675.1	NP_001316604.1	cullin-associated NEDD8-dissociated protein 1 isoform 2
NM_001329676.2	NP_001316605.1	cullin-associated NEDD8-dissociated protein 1 isoform 3
NM_001329676.1	NP_001316605.1	cullin-associated NEDD8-dissociated protein 1 isoform 3
NM_001329674.2	NP_001316603.1	cullin-associated NEDD8-dissociated protein 1 isoform 2
NM_018448.5	NP_060918.2	cullin-associated NEDD8-dissociated protein 1 isoform 1
NM_018448.4	NP_060918.2	cullin-associated NEDD8-dissociated protein 1 isoform 1
NM_001329675.2	NP_001316604.1	cullin-associated NEDD8-dissociated protein 1 isoform 2

Pathways from BioSystems

Homologies

Mus musculus (house mouse)	Cand1	NP_082270.1
Gallus gallus (chicken)	CAND1	XP_416078.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009970	XP_319106.4
Xenopus tropicalis (tropical clawed frog)	cand1	NP_001182702.1
	CAND1	XP_001106780.2
Bos taurus (cattle)	CAND1	NP_001094643.1
Homo sapiens (human)	CAND1	NP_060918.2
Pan troglodytes (chimpanzee)	CAND1	XP_509204.3
Canis lupus familiaris (dog)	CAND1	XP_005625653.1
Danio rerio (zebrafish)	cand1	NP_998650.1
Caenorhabditis elegans (roundworm)	cand-1	NP_507244.1
Rattus norvegicus (Norway rat)	Cand1	NP_446456.1
Drosophila melanogaster (fruit fly)	Cand1	NP_001260333.1

Gene Ontology
Bibliography

Related articles in PubMed

miR-33a inhibits cell proliferation and invasion by targeting CAND1 in lung cancer.
Kang M, Li Y, Zhao Y, He S, Shi J
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico(2017 Sep)

Disclosing the Interaction of Carbonic Anhydrase IX with Cullin-Associated NEDD8-Dissociated Protein 1 by Molecular Modeling and Integrated Binding Measurements.
Buonanno M, Langella E, Zambrano N, Succoio M, Sasso E, Alterio V, Di Fiore A, Sandomenico A, Supuran CT, Scaloni A, Monti SM, De Simone G
ACS chemical biology12(6)1460-1465(2017 Jun)

CAND1 exchange factor promotes Keap1 integration into cullin 3-RING ubiquitin ligase during adipogenesis.
Dubiel D, Ordemann J, Pratschke J, Dubiel W, Naumann M
The international journal of biochemistry & cell biology6695-100(2015 Sep)

Cand1 promotes assembly of new SCF complexes through dynamic exchange of F box proteins.
Pierce NW, Lee JE, Liu X, Sweredoski MJ, Graham RL, Larimore EA, Rome M, Zheng N, Clurman BE, Hess S, Shan SO, Deshaies RJ
Cell153(1)206-15(2013 Mar)

COMMD1 (copper metabolism MURR1 domain-containing protein 1) regulates Cullin RING ligases by preventing CAND1 (Cullin-associated Nedd8-dissociated protein 1) binding.
Mao X, Gluck N, Chen B, Starokadomskyy P, Li H, Maine GN, Burstein E
The Journal of biological chemistry286(37)32355-65(2011 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

describe the identification of the structural determinants responsible for the CA IX/CAND1 interaction
Title: Disclosing the Interaction of Carbonic Anhydrase IX with Cullin-Associated NEDD8-Dissociated Protein 1 by Molecular Modeling and Integrated Binding Measurements.

Data show that the differentiation of LiSa-2 preadipocytes is associated with an increase of cullin-associated and neddylation-dissociated 1 (CAND1), COP9 signalosome (CSN), neddylated cullin 3 (Cul3) and the BTB protein Keap1.
Title: CAND1 exchange factor promotes Keap1 integration into cullin 3-RING ubiquitin ligase during adipogenesis.

We demonstrate that the accumulation of p27 is associated with an increase of CAND1 and a decrease of Skp2 during adipogenesis of human LiSa-2 preadipocytes. CAND1 knockdown reduces p27 and blocks adipogenesis.
Title: CAND1-dependent control of cullin 1-RING Ub ligases is essential for adipogenesis.

Study shows that Cand1 can unambiguously stimulate SCF activity in vitro by enabling an F box protein-Skp1 complex to access Cul1 that was previously occupied by a different F box protein-Skp1 complex, and that Cand1 promotes assembly in vivo of new F box proteins with pre-existing Cul1 molecules.
Title: Cand1 promotes assembly of new SCF complexes through dynamic exchange of F box proteins.

CAND1 promotes PLK4-mediated centriole overduplication and is frequently disrupted in prostate cancer.
Title: CAND1 promotes PLK4-mediated centriole overduplication and is frequently disrupted in prostate cancer.

The following CAND1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CAND1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu101665	NM_001329674.2 Latest version!	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu101665	NM_001329675.2 Latest version!	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu101809	NM_001329676.2 Latest version!	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu31371	NM_018448.5 Latest version!	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$384.30 $549.00
View Old Accession Versions >>
OHu101809	NM_001329676.1	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu101665	NM_001329674.1	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
OHu31371	NM_018448.4	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$384.30 $549.00
OHu101665	NM_001329675.1	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$643.30 $919.00
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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CAND1 ( NM_001329674.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316603.1 Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu101665
Clone ID Related Accession (Same CDS sequence)	NM_001329674.1 , NM_001329675.1 , NM_001329674.2 , NM_001329675.2
Accession Version	NM_001329674.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-01
Organism	Homo sapiens(human)
Product	cullin-associated NEDD8-dissociated protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012518.19, AC078983.17 and AA766567.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.168437.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820689, SAMN03267750 [ECO:0000348] ##Evidence-Data-END##

ATGGCTACAA ATGATTTGAT GACGGAACTG CAGAAAGATT CCATCAAGTT GGATGATGAT 
AGTGAAAGGA AAGTAGTGAA AATGATTTTG AAGTTATTGG AAGATAAAAA TGGAGAGGTA 
CAGAATTTAG CTGTCAAATG TCTTGGTCCT TTAGTGAGTA AAGTGAAAGA ATACCAAGTA 
GAGACAATTG TAGATACCCT CTGCACTAAC ATGCTTTCTG ATAAAGAACA ACTTCGAGAC 
ATTTCAAGTA TTGGTCTTAA AACAGTAATT GGAGAACTTC CTCCAGCTTC CAGTGGCTCT 
GCATTAGCTG CTAATGTATG TAAAAAGATT ACTGGACGTC TTACAAGTGC AATAGCAAAA 
CAGGAAGATG TCTCTGTTCA GCTAGAAGCC TTGGATATTA TGGCTGATAT GTTGAGCAGG 
CAAGGAGGAC TTCTTGTTAA TTTCCATCCT TCAATTCTGA CCTGTCTACT TCCCCAGTTG 
ACCAGCCCTA GACTTGCAGT GAGGAAAAGA ACCATTATCG CTCTTGGCCA TCTGGTTATG 
AGCTGTGGAA ATATAGTTTT TGTAGATCTT ATTGAACATC TGTTGTCAGA GTTGTCCAAA 
AATGATTCTA TGTCAACAAC AAGAACCTAC ATACAATGTA TTGCTGCTAT TAGTAGGCAA 
GCTGGTCATA GAATAGGTGA ATACCTTGAG AAGATAATTC CTTTGGTGGT AAAATTTTGC 
AATGTAGATG ATGATGAATT AAGAGAGTAC TGTATTCAAG CCTTTGAATC ATTTGTAAGA 
AGATGTCCTA AGGAAGTATA TCCTCATGTT TCTACCATTA TAAATATTTG TCTTAAATAT 
CTTACCTATG ATCCAAATTA TAATTACGAT GATGAAGATG AAGATGAAAA TGCAATGGAT 
GCTGATGGTG GTGATGATGA TGATCAAGGG AGTGATGATG AATACAGTGA TGATGATGAC 
ATGAGTTGGA AAGTGAGACG TGCAGCTGCG AAGTGCTTGG ATGCTGTAGT TAGCACAAGG 
CATGAAATGC TTCCAGAATT CTACAAGACC GTCTCTCCTG CACTAATATC CAGATTTAAA 
GAGCGTGAAG AGAATGTAAA GGCAGATGTT TTTCACGCAT ACCTTTCTCT TTTGAAGCAA 
ACTCGTCCTG TACAAAGTTG GCTATGTGAC CCTGATGCAA TGGAGCAGGG AGAAACACCT 
TTAACAATGC TTCAGAGTCA GGTTCCCAAC ATTGTTAAAG CTCTTCACAA ACAGATGAAA 
GAAAAAAGTG TGAAGACCCG ACAGTGTTGT TTTAACATGT TAACTGAGCT GGTAAATGTA 
TTACCTGGGG CCCTAACTCA ACACATTCCT GTACTTGTAC CAGGAATCAT TTTCTCACTG 
AATGATAAAT CAAGCTCATC GAATTTGAAG ATCGATGCTT TGTCATGTCT ATACGTAATC 
CTCTGTAACC ATTCTCCTCA AGTCTTCCAT CCTCACGTTC AGGCTTTGGT TCCTCCAGTG 
GTGGCTTGTG TTGGAGACCC ATTTTACAAA ATTACATCTG AAGCACTTCT TGTTACTCAA 
CAGCTTGTCA AAGTAATTCG TCCTTTAGAT CAGCCTTCCT CGTTTGATGC AACTCCTTAT 
ATCAAAGATC TATTTACCTG TACCATTAAG AGATTAAAAG CAGCTGACAT TGATCAGGAA 
GTCAAGGAAA GGGCTATTTC CTGTATGGGA CAAATTATTT GCAACCTTGG AGACAATTTG 
GGTTCTGACT TGCCTAATAC ACTTCAGATT TTCTTGGAGA GACTAAAGAA TGAAATTACC 
AGGTTAACTA CAGTAAAGGC ATTGACACTG ATTGCTGGGT CACCTTTGAA GATAGATTTG 
AGGCCTGTTC TGGGAGAAGG GGTTCCTATC CTTGCTTCAT TTCTTAGAAA AAACCAGAGA 
GCTTTGAAAC TGGGTACTCT TTCTGCCCTT GATATTCTAA TAAAAAACTA TAGTGACAGC 
TTGACAGCTG CCATGATTGA TGCAGTTCTA GATGAGCTCC CACCTCTTAT CAGCGAAAGT 
GATATGCATG TTTCACAAAT GGCCATCAGT TTTCTTACCA CTTTGGCAAA AGTATATCCC 
TCCTCCCTTT CAAAGATAAG TGGATCCATT CTCAATGAAC TTATTGGACT TGTGAGATCA 
CCCTTATTGC AGGGGGGAGC TCTTAGTGCC ATGCTAGACT TTTTCCAAGC TCTGGTTGTC 
ACTGGAACAA ATAATTTAGG ATACATGGAT TTGTTGCGCA TGCTGACTGG TCCAGTTTAC 
TCTCAGAGCA CAGCTCTTAC TCATAAGCAG TCTTATTATT CCATTGCCAA ATGTGTAGCT 
GCCCTTACTC GAGCATGCCC TAAAGAGGGA CCAGCTGTAG TAGGTCAGTT TATTCAAGAT 
GTCAAGAACT CAAGGTCTAC AGATTCCATT CGTCTCTTAG CTCTACTTTC TCTTGGAGAA 
GTTGGGCATC ATATTGACTT AAGTGGACAG TTGGAACTAA AATCTGTAAT ACTAGAAGCT 
TTCTCATCTC CTAGTGAAGA AGTCAAATCA GCTGCATCCT ATGCATTAGG CAGCATTAGT 
GTGGGCAACC TTCCTGAATA TCTGCCGTTT GTCCTGCAAG AAATAACTAG TCAACCCAAA 
AGGCAGTATC TTTTACTTCA TTCCTTGAAG GAAATTATTA GCTCTGCATC AGTGGTGGGC 
CTTAAACCAT ATGTTGAAAA CATCTGGGCC TTATTACTAA AGCACTGTGA GTGTGCAGAG 
GAAGGAACCA GAAATGTTGT TGCTGAATGT CTAGGAAAAC TCACTCTAAT TGATCCAGAA 
ACTCTCCTTC CACGGCTTAA GGGGTACTTG ATATCAGGCT CATCATATGC CCGAAGCTCA 
GTGGTTACGG CTGTGAAATT TACAATTTCT GACCATCCAC AACCTATTGA TCCACTGTTA 
AAGAACTGCA TAGGTGATTT CCTAAAAACT TTGGAAGACC CAGATTTGAA TGTGAGAAGA 
GTAGCCTTGG TCACATTTAA TTCAGCAGCA CATAACAAGC CATCATTAAT AAGGGATCTA 
TTGGATACTG TTCTTCCACA TCTTTACAAT GAAACAAAAG TTAGAAAGGA GCTTATAAGA 
GAGGTAGAAA TGGGTCCATT TAAACATACG GTTGATGATG GTCTGGATAT TAGAAAGGCA 
GCATTTGAGT GTATGTACAC ACTTCTAGAC AGTTGTCTTG ATAGACTTGA TATCTTTGAA 
TTTCTAAATC ATGTTGAAGA TGGTTTGAAG GACCATTATG ATATTAAGAT GCTGACATTT 
TTAATGTTGG TGAGACTGTC TACCCTTTGT CCAAGTGCAG TACTGCAGAG GTTGGACCGA 
CTTGTTGAGC CATTACGTGC AACATGTACA ACTAAGGTAA AGGCAAACTC AGTAAAGCAG 
GAGTTTGAAA AACAAGATGA ATTAAAGCGA TCTGCCATGA GAGCAGTAGC AGCACTGCTA 
ACCATTCCAG AAGCAGAGAA GAGTCCACTG ATGAGTGAAT TCCAGTCACA GATCAGTTCT 
AACCCTGAGC TGGCGGCTAT CTTTGAAAGT ATCCAGAAAG ATTCATCATC TACTAACTTG 
GAATCAATGG ACACTAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316603.1
CDS	637..4257
Translation	MATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS

Target ORF information:

RefSeq Version	NM_001329674.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329674.1

ORF Insert Sequence:

ATGGCTACAA ATGATTTGAT GACGGAACTG CAGAAAGATT CCATCAAGTT GGATGATGAT 
AGTGAAAGGA AAGTAGTGAA AATGATTTTG AAGTTATTGG AAGATAAAAA TGGAGAGGTA 
CAGAATTTAG CTGTCAAATG TCTTGGTCCT TTAGTGAGTA AAGTGAAAGA ATACCAAGTA 
GAGACAATTG TAGATACCCT CTGCACTAAC ATGCTTTCTG ATAAAGAACA ACTTCGAGAC 
ATTTCAAGTA TTGGTCTTAA AACAGTAATT GGAGAACTTC CTCCAGCTTC CAGTGGCTCT 
GCATTAGCTG CTAATGTATG TAAAAAGATT ACTGGACGTC TTACAAGTGC AATAGCAAAA 
CAGGAAGATG TCTCTGTTCA GCTAGAAGCC TTGGATATTA TGGCTGATAT GTTGAGCAGG 
CAAGGAGGAC TTCTTGTTAA TTTCCATCCT TCAATTCTGA CCTGTCTACT TCCCCAGTTG 
ACCAGCCCTA GACTTGCAGT GAGGAAAAGA ACCATTATCG CTCTTGGCCA TCTGGTTATG 
AGCTGTGGAA ATATAGTTTT TGTAGATCTT ATTGAACATC TGTTGTCAGA GTTGTCCAAA 
AATGATTCTA TGTCAACAAC AAGAACCTAC ATACAATGTA TTGCTGCTAT TAGTAGGCAA 
GCTGGTCATA GAATAGGTGA ATACCTTGAG AAGATAATTC CTTTGGTGGT AAAATTTTGC 
AATGTAGATG ATGATGAATT AAGAGAGTAC TGTATTCAAG CCTTTGAATC ATTTGTAAGA 
AGATGTCCTA AGGAAGTATA TCCTCATGTT TCTACCATTA TAAATATTTG TCTTAAATAT 
CTTACCTATG ATCCAAATTA TAATTACGAT GATGAAGATG AAGATGAAAA TGCAATGGAT 
GCTGATGGTG GTGATGATGA TGATCAAGGG AGTGATGATG AATACAGTGA TGATGATGAC 
ATGAGTTGGA AAGTGAGACG TGCAGCTGCG AAGTGCTTGG ATGCTGTAGT TAGCACAAGG 
CATGAAATGC TTCCAGAATT CTACAAGACC GTCTCTCCTG CACTAATATC CAGATTTAAA 
GAGCGTGAAG AGAATGTAAA GGCAGATGTT TTTCACGCAT ACCTTTCTCT TTTGAAGCAA 
ACTCGTCCTG TACAAAGTTG GCTATGTGAC CCTGATGCAA TGGAGCAGGG AGAAACACCT 
TTAACAATGC TTCAGAGTCA GGTTCCCAAC ATTGTTAAAG CTCTTCACAA ACAGATGAAA 
GAAAAAAGTG TGAAGACCCG ACAGTGTTGT TTTAACATGT TAACTGAGCT GGTAAATGTA 
TTACCTGGGG CCCTAACTCA ACACATTCCT GTACTTGTAC CAGGAATCAT TTTCTCACTG 
AATGATAAAT CAAGCTCATC GAATTTGAAG ATCGATGCTT TGTCATGTCT ATACGTAATC 
CTCTGTAACC ATTCTCCTCA AGTCTTCCAT CCTCACGTTC AGGCTTTGGT TCCTCCAGTG 
GTGGCTTGTG TTGGAGACCC ATTTTACAAA ATTACATCTG AAGCACTTCT TGTTACTCAA 
CAGCTTGTCA AAGTAATTCG TCCTTTAGAT CAGCCTTCCT CGTTTGATGC AACTCCTTAT 
ATCAAAGATC TATTTACCTG TACCATTAAG AGATTAAAAG CAGCTGACAT TGATCAGGAA 
GTCAAGGAAA GGGCTATTTC CTGTATGGGA CAAATTATTT GCAACCTTGG AGACAATTTG 
GGTTCTGACT TGCCTAATAC ACTTCAGATT TTCTTGGAGA GACTAAAGAA TGAAATTACC 
AGGTTAACTA CAGTAAAGGC ATTGACACTG ATTGCTGGGT CACCTTTGAA GATAGATTTG 
AGGCCTGTTC TGGGAGAAGG GGTTCCTATC CTTGCTTCAT TTCTTAGAAA AAACCAGAGA 
GCTTTGAAAC TGGGTACTCT TTCTGCCCTT GATATTCTAA TAAAAAACTA TAGTGACAGC 
TTGACAGCTG CCATGATTGA TGCAGTTCTA GATGAGCTCC CACCTCTTAT CAGCGAAAGT 
GATATGCATG TTTCACAAAT GGCCATCAGT TTTCTTACCA CTTTGGCAAA AGTATATCCC 
TCCTCCCTTT CAAAGATAAG TGGATCCATT CTCAATGAAC TTATTGGACT TGTGAGATCA 
CCCTTATTGC AGGGGGGAGC TCTTAGTGCC ATGCTAGACT TTTTCCAAGC TCTGGTTGTC 
ACTGGAACAA ATAATTTAGG ATACATGGAT TTGTTGCGCA TGCTGACTGG TCCAGTTTAC 
TCTCAGAGCA CAGCTCTTAC TCATAAGCAG TCTTATTATT CCATTGCCAA ATGTGTAGCT 
GCCCTTACTC GAGCATGCCC TAAAGAGGGA CCAGCTGTAG TAGGTCAGTT TATTCAAGAT 
GTCAAGAACT CAAGGTCTAC AGATTCCATT CGTCTCTTAG CTCTACTTTC TCTTGGAGAA 
GTTGGGCATC ATATTGACTT AAGTGGACAG TTGGAACTAA AATCTGTAAT ACTAGAAGCT 
TTCTCATCTC CTAGTGAAGA AGTCAAATCA GCTGCATCCT ATGCATTAGG CAGCATTAGT 
GTGGGCAACC TTCCTGAATA TCTGCCGTTT GTCCTGCAAG AAATAACTAG TCAACCCAAA 
AGGCAGTATC TTTTACTTCA TTCCTTGAAG GAAATTATTA GCTCTGCATC AGTGGTGGGC 
CTTAAACCAT ATGTTGAAAA CATCTGGGCC TTATTACTAA AGCACTGTGA GTGTGCAGAG 
GAAGGAACCA GAAATGTTGT TGCTGAATGT CTAGGAAAAC TCACTCTAAT TGATCCAGAA 
ACTCTCCTTC CACGGCTTAA GGGGTACTTG ATATCAGGCT CATCATATGC CCGAAGCTCA 
GTGGTTACGG CTGTGAAATT TACAATTTCT GACCATCCAC AACCTATTGA TCCACTGTTA 
AAGAACTGCA TAGGTGATTT CCTAAAAACT TTGGAAGACC CAGATTTGAA TGTGAGAAGA 
GTAGCCTTGG TCACATTTAA TTCAGCAGCA CATAACAAGC CATCATTAAT AAGGGATCTA 
TTGGATACTG TTCTTCCACA TCTTTACAAT GAAACAAAAG TTAGAAAGGA GCTTATAAGA 
GAGGTAGAAA TGGGTCCATT TAAACATACG GTTGATGATG GTCTGGATAT TAGAAAGGCA 
GCATTTGAGT GTATGTACAC ACTTCTAGAC AGTTGTCTTG ATAGACTTGA TATCTTTGAA 
TTTCTAAATC ATGTTGAAGA TGGTTTGAAG GACCATTATG ATATTAAGAT GCTGACATTT 
TTAATGTTGG TGAGACTGTC TACCCTTTGT CCAAGTGCAG TACTGCAGAG GTTGGACCGA 
CTTGTTGAGC CATTACGTGC AACATGTACA ACTAAGGTAA AGGCAAACTC AGTAAAGCAG 
GAGTTTGAAA AACAAGATGA ATTAAAGCGA TCTGCCATGA GAGCAGTAGC AGCACTGCTA 
ACCATTCCAG AAGCAGAGAA GAGTCCACTG ATGAGTGAAT TCCAGTCACA GATCAGTTCT 
AACCCTGAGC TGGCGGCTAT CTTTGAAAGT ATCCAGAAAG ATTCATCATC TACTAACTTG 
GAATCAATGG ACACTAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAND1 ( NM_001329675.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316604.1 Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 3, mRNA.

Price & Availability↑

CloneID	OHu101665
Clone ID Related Accession (Same CDS sequence)	NM_001329674.1 , NM_001329675.1 , NM_001329674.2 , NM_001329675.2
Accession Version	NM_001329675.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-01
Organism	Homo sapiens(human)
Product	cullin-associated NEDD8-dissociated protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012518.19, AC078983.17 and AA766567.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.89650.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTACAA ATGATTTGAT GACGGAACTG CAGAAAGATT CCATCAAGTT GGATGATGAT 
AGTGAAAGGA AAGTAGTGAA AATGATTTTG AAGTTATTGG AAGATAAAAA TGGAGAGGTA 
CAGAATTTAG CTGTCAAATG TCTTGGTCCT TTAGTGAGTA AAGTGAAAGA ATACCAAGTA 
GAGACAATTG TAGATACCCT CTGCACTAAC ATGCTTTCTG ATAAAGAACA ACTTCGAGAC 
ATTTCAAGTA TTGGTCTTAA AACAGTAATT GGAGAACTTC CTCCAGCTTC CAGTGGCTCT 
GCATTAGCTG CTAATGTATG TAAAAAGATT ACTGGACGTC TTACAAGTGC AATAGCAAAA 
CAGGAAGATG TCTCTGTTCA GCTAGAAGCC TTGGATATTA TGGCTGATAT GTTGAGCAGG 
CAAGGAGGAC TTCTTGTTAA TTTCCATCCT TCAATTCTGA CCTGTCTACT TCCCCAGTTG 
ACCAGCCCTA GACTTGCAGT GAGGAAAAGA ACCATTATCG CTCTTGGCCA TCTGGTTATG 
AGCTGTGGAA ATATAGTTTT TGTAGATCTT ATTGAACATC TGTTGTCAGA GTTGTCCAAA 
AATGATTCTA TGTCAACAAC AAGAACCTAC ATACAATGTA TTGCTGCTAT TAGTAGGCAA 
GCTGGTCATA GAATAGGTGA ATACCTTGAG AAGATAATTC CTTTGGTGGT AAAATTTTGC 
AATGTAGATG ATGATGAATT AAGAGAGTAC TGTATTCAAG CCTTTGAATC ATTTGTAAGA 
AGATGTCCTA AGGAAGTATA TCCTCATGTT TCTACCATTA TAAATATTTG TCTTAAATAT 
CTTACCTATG ATCCAAATTA TAATTACGAT GATGAAGATG AAGATGAAAA TGCAATGGAT 
GCTGATGGTG GTGATGATGA TGATCAAGGG AGTGATGATG AATACAGTGA TGATGATGAC 
ATGAGTTGGA AAGTGAGACG TGCAGCTGCG AAGTGCTTGG ATGCTGTAGT TAGCACAAGG 
CATGAAATGC TTCCAGAATT CTACAAGACC GTCTCTCCTG CACTAATATC CAGATTTAAA 
GAGCGTGAAG AGAATGTAAA GGCAGATGTT TTTCACGCAT ACCTTTCTCT TTTGAAGCAA 
ACTCGTCCTG TACAAAGTTG GCTATGTGAC CCTGATGCAA TGGAGCAGGG AGAAACACCT 
TTAACAATGC TTCAGAGTCA GGTTCCCAAC ATTGTTAAAG CTCTTCACAA ACAGATGAAA 
GAAAAAAGTG TGAAGACCCG ACAGTGTTGT TTTAACATGT TAACTGAGCT GGTAAATGTA 
TTACCTGGGG CCCTAACTCA ACACATTCCT GTACTTGTAC CAGGAATCAT TTTCTCACTG 
AATGATAAAT CAAGCTCATC GAATTTGAAG ATCGATGCTT TGTCATGTCT ATACGTAATC 
CTCTGTAACC ATTCTCCTCA AGTCTTCCAT CCTCACGTTC AGGCTTTGGT TCCTCCAGTG 
GTGGCTTGTG TTGGAGACCC ATTTTACAAA ATTACATCTG AAGCACTTCT TGTTACTCAA 
CAGCTTGTCA AAGTAATTCG TCCTTTAGAT CAGCCTTCCT CGTTTGATGC AACTCCTTAT 
ATCAAAGATC TATTTACCTG TACCATTAAG AGATTAAAAG CAGCTGACAT TGATCAGGAA 
GTCAAGGAAA GGGCTATTTC CTGTATGGGA CAAATTATTT GCAACCTTGG AGACAATTTG 
GGTTCTGACT TGCCTAATAC ACTTCAGATT TTCTTGGAGA GACTAAAGAA TGAAATTACC 
AGGTTAACTA CAGTAAAGGC ATTGACACTG ATTGCTGGGT CACCTTTGAA GATAGATTTG 
AGGCCTGTTC TGGGAGAAGG GGTTCCTATC CTTGCTTCAT TTCTTAGAAA AAACCAGAGA 
GCTTTGAAAC TGGGTACTCT TTCTGCCCTT GATATTCTAA TAAAAAACTA TAGTGACAGC 
TTGACAGCTG CCATGATTGA TGCAGTTCTA GATGAGCTCC CACCTCTTAT CAGCGAAAGT 
GATATGCATG TTTCACAAAT GGCCATCAGT TTTCTTACCA CTTTGGCAAA AGTATATCCC 
TCCTCCCTTT CAAAGATAAG TGGATCCATT CTCAATGAAC TTATTGGACT TGTGAGATCA 
CCCTTATTGC AGGGGGGAGC TCTTAGTGCC ATGCTAGACT TTTTCCAAGC TCTGGTTGTC 
ACTGGAACAA ATAATTTAGG ATACATGGAT TTGTTGCGCA TGCTGACTGG TCCAGTTTAC 
TCTCAGAGCA CAGCTCTTAC TCATAAGCAG TCTTATTATT CCATTGCCAA ATGTGTAGCT 
GCCCTTACTC GAGCATGCCC TAAAGAGGGA CCAGCTGTAG TAGGTCAGTT TATTCAAGAT 
GTCAAGAACT CAAGGTCTAC AGATTCCATT CGTCTCTTAG CTCTACTTTC TCTTGGAGAA 
GTTGGGCATC ATATTGACTT AAGTGGACAG TTGGAACTAA AATCTGTAAT ACTAGAAGCT 
TTCTCATCTC CTAGTGAAGA AGTCAAATCA GCTGCATCCT ATGCATTAGG CAGCATTAGT 
GTGGGCAACC TTCCTGAATA TCTGCCGTTT GTCCTGCAAG AAATAACTAG TCAACCCAAA 
AGGCAGTATC TTTTACTTCA TTCCTTGAAG GAAATTATTA GCTCTGCATC AGTGGTGGGC 
CTTAAACCAT ATGTTGAAAA CATCTGGGCC TTATTACTAA AGCACTGTGA GTGTGCAGAG 
GAAGGAACCA GAAATGTTGT TGCTGAATGT CTAGGAAAAC TCACTCTAAT TGATCCAGAA 
ACTCTCCTTC CACGGCTTAA GGGGTACTTG ATATCAGGCT CATCATATGC CCGAAGCTCA 
GTGGTTACGG CTGTGAAATT TACAATTTCT GACCATCCAC AACCTATTGA TCCACTGTTA 
AAGAACTGCA TAGGTGATTT CCTAAAAACT TTGGAAGACC CAGATTTGAA TGTGAGAAGA 
GTAGCCTTGG TCACATTTAA TTCAGCAGCA CATAACAAGC CATCATTAAT AAGGGATCTA 
TTGGATACTG TTCTTCCACA TCTTTACAAT GAAACAAAAG TTAGAAAGGA GCTTATAAGA 
GAGGTAGAAA TGGGTCCATT TAAACATACG GTTGATGATG GTCTGGATAT TAGAAAGGCA 
GCATTTGAGT GTATGTACAC ACTTCTAGAC AGTTGTCTTG ATAGACTTGA TATCTTTGAA 
TTTCTAAATC ATGTTGAAGA TGGTTTGAAG GACCATTATG ATATTAAGAT GCTGACATTT 
TTAATGTTGG TGAGACTGTC TACCCTTTGT CCAAGTGCAG TACTGCAGAG GTTGGACCGA 
CTTGTTGAGC CATTACGTGC AACATGTACA ACTAAGGTAA AGGCAAACTC AGTAAAGCAG 
GAGTTTGAAA AACAAGATGA ATTAAAGCGA TCTGCCATGA GAGCAGTAGC AGCACTGCTA 
ACCATTCCAG AAGCAGAGAA GAGTCCACTG ATGAGTGAAT TCCAGTCACA GATCAGTTCT 
AACCCTGAGC TGGCGGCTAT CTTTGAAAGT ATCCAGAAAG ATTCATCATC TACTAACTTG 
GAATCAATGG ACACTAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316604.1
CDS	631..4251
Translation	MATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS

Target ORF information:

RefSeq Version	NM_001329675.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329675.1

ORF Insert Sequence:

ATGGCTACAA ATGATTTGAT GACGGAACTG CAGAAAGATT CCATCAAGTT GGATGATGAT 
AGTGAAAGGA AAGTAGTGAA AATGATTTTG AAGTTATTGG AAGATAAAAA TGGAGAGGTA 
CAGAATTTAG CTGTCAAATG TCTTGGTCCT TTAGTGAGTA AAGTGAAAGA ATACCAAGTA 
GAGACAATTG TAGATACCCT CTGCACTAAC ATGCTTTCTG ATAAAGAACA ACTTCGAGAC 
ATTTCAAGTA TTGGTCTTAA AACAGTAATT GGAGAACTTC CTCCAGCTTC CAGTGGCTCT 
GCATTAGCTG CTAATGTATG TAAAAAGATT ACTGGACGTC TTACAAGTGC AATAGCAAAA 
CAGGAAGATG TCTCTGTTCA GCTAGAAGCC TTGGATATTA TGGCTGATAT GTTGAGCAGG 
CAAGGAGGAC TTCTTGTTAA TTTCCATCCT TCAATTCTGA CCTGTCTACT TCCCCAGTTG 
ACCAGCCCTA GACTTGCAGT GAGGAAAAGA ACCATTATCG CTCTTGGCCA TCTGGTTATG 
AGCTGTGGAA ATATAGTTTT TGTAGATCTT ATTGAACATC TGTTGTCAGA GTTGTCCAAA 
AATGATTCTA TGTCAACAAC AAGAACCTAC ATACAATGTA TTGCTGCTAT TAGTAGGCAA 
GCTGGTCATA GAATAGGTGA ATACCTTGAG AAGATAATTC CTTTGGTGGT AAAATTTTGC 
AATGTAGATG ATGATGAATT AAGAGAGTAC TGTATTCAAG CCTTTGAATC ATTTGTAAGA 
AGATGTCCTA AGGAAGTATA TCCTCATGTT TCTACCATTA TAAATATTTG TCTTAAATAT 
CTTACCTATG ATCCAAATTA TAATTACGAT GATGAAGATG AAGATGAAAA TGCAATGGAT 
GCTGATGGTG GTGATGATGA TGATCAAGGG AGTGATGATG AATACAGTGA TGATGATGAC 
ATGAGTTGGA AAGTGAGACG TGCAGCTGCG AAGTGCTTGG ATGCTGTAGT TAGCACAAGG 
CATGAAATGC TTCCAGAATT CTACAAGACC GTCTCTCCTG CACTAATATC CAGATTTAAA 
GAGCGTGAAG AGAATGTAAA GGCAGATGTT TTTCACGCAT ACCTTTCTCT TTTGAAGCAA 
ACTCGTCCTG TACAAAGTTG GCTATGTGAC CCTGATGCAA TGGAGCAGGG AGAAACACCT 
TTAACAATGC TTCAGAGTCA GGTTCCCAAC ATTGTTAAAG CTCTTCACAA ACAGATGAAA 
GAAAAAAGTG TGAAGACCCG ACAGTGTTGT TTTAACATGT TAACTGAGCT GGTAAATGTA 
TTACCTGGGG CCCTAACTCA ACACATTCCT GTACTTGTAC CAGGAATCAT TTTCTCACTG 
AATGATAAAT CAAGCTCATC GAATTTGAAG ATCGATGCTT TGTCATGTCT ATACGTAATC 
CTCTGTAACC ATTCTCCTCA AGTCTTCCAT CCTCACGTTC AGGCTTTGGT TCCTCCAGTG 
GTGGCTTGTG TTGGAGACCC ATTTTACAAA ATTACATCTG AAGCACTTCT TGTTACTCAA 
CAGCTTGTCA AAGTAATTCG TCCTTTAGAT CAGCCTTCCT CGTTTGATGC AACTCCTTAT 
ATCAAAGATC TATTTACCTG TACCATTAAG AGATTAAAAG CAGCTGACAT TGATCAGGAA 
GTCAAGGAAA GGGCTATTTC CTGTATGGGA CAAATTATTT GCAACCTTGG AGACAATTTG 
GGTTCTGACT TGCCTAATAC ACTTCAGATT TTCTTGGAGA GACTAAAGAA TGAAATTACC 
AGGTTAACTA CAGTAAAGGC ATTGACACTG ATTGCTGGGT CACCTTTGAA GATAGATTTG 
AGGCCTGTTC TGGGAGAAGG GGTTCCTATC CTTGCTTCAT TTCTTAGAAA AAACCAGAGA 
GCTTTGAAAC TGGGTACTCT TTCTGCCCTT GATATTCTAA TAAAAAACTA TAGTGACAGC 
TTGACAGCTG CCATGATTGA TGCAGTTCTA GATGAGCTCC CACCTCTTAT CAGCGAAAGT 
GATATGCATG TTTCACAAAT GGCCATCAGT TTTCTTACCA CTTTGGCAAA AGTATATCCC 
TCCTCCCTTT CAAAGATAAG TGGATCCATT CTCAATGAAC TTATTGGACT TGTGAGATCA 
CCCTTATTGC AGGGGGGAGC TCTTAGTGCC ATGCTAGACT TTTTCCAAGC TCTGGTTGTC 
ACTGGAACAA ATAATTTAGG ATACATGGAT TTGTTGCGCA TGCTGACTGG TCCAGTTTAC 
TCTCAGAGCA CAGCTCTTAC TCATAAGCAG TCTTATTATT CCATTGCCAA ATGTGTAGCT 
GCCCTTACTC GAGCATGCCC TAAAGAGGGA CCAGCTGTAG TAGGTCAGTT TATTCAAGAT 
GTCAAGAACT CAAGGTCTAC AGATTCCATT CGTCTCTTAG CTCTACTTTC TCTTGGAGAA 
GTTGGGCATC ATATTGACTT AAGTGGACAG TTGGAACTAA AATCTGTAAT ACTAGAAGCT 
TTCTCATCTC CTAGTGAAGA AGTCAAATCA GCTGCATCCT ATGCATTAGG CAGCATTAGT 
GTGGGCAACC TTCCTGAATA TCTGCCGTTT GTCCTGCAAG AAATAACTAG TCAACCCAAA 
AGGCAGTATC TTTTACTTCA TTCCTTGAAG GAAATTATTA GCTCTGCATC AGTGGTGGGC 
CTTAAACCAT ATGTTGAAAA CATCTGGGCC TTATTACTAA AGCACTGTGA GTGTGCAGAG 
GAAGGAACCA GAAATGTTGT TGCTGAATGT CTAGGAAAAC TCACTCTAAT TGATCCAGAA 
ACTCTCCTTC CACGGCTTAA GGGGTACTTG ATATCAGGCT CATCATATGC CCGAAGCTCA 
GTGGTTACGG CTGTGAAATT TACAATTTCT GACCATCCAC AACCTATTGA TCCACTGTTA 
AAGAACTGCA TAGGTGATTT CCTAAAAACT TTGGAAGACC CAGATTTGAA TGTGAGAAGA 
GTAGCCTTGG TCACATTTAA TTCAGCAGCA CATAACAAGC CATCATTAAT AAGGGATCTA 
TTGGATACTG TTCTTCCACA TCTTTACAAT GAAACAAAAG TTAGAAAGGA GCTTATAAGA 
GAGGTAGAAA TGGGTCCATT TAAACATACG GTTGATGATG GTCTGGATAT TAGAAAGGCA 
GCATTTGAGT GTATGTACAC ACTTCTAGAC AGTTGTCTTG ATAGACTTGA TATCTTTGAA 
TTTCTAAATC ATGTTGAAGA TGGTTTGAAG GACCATTATG ATATTAAGAT GCTGACATTT 
TTAATGTTGG TGAGACTGTC TACCCTTTGT CCAAGTGCAG TACTGCAGAG GTTGGACCGA 
CTTGTTGAGC CATTACGTGC AACATGTACA ACTAAGGTAA AGGCAAACTC AGTAAAGCAG 
GAGTTTGAAA AACAAGATGA ATTAAAGCGA TCTGCCATGA GAGCAGTAGC AGCACTGCTA 
ACCATTCCAG AAGCAGAGAA GAGTCCACTG ATGAGTGAAT TCCAGTCACA GATCAGTTCT 
AACCCTGAGC TGGCGGCTAT CTTTGAAAGT ATCCAGAAAG ATTCATCATC TACTAACTTG 
GAATCAATGG ACACTAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAND1 ( NM_001329674.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316603.1 Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 2, mRNA.

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CloneID	OHu101665
Clone ID Related Accession (Same CDS sequence)	NM_001329674.1 , NM_001329675.1 , NM_001329674.2 , NM_001329675.2
Accession Version	NM_001329674.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	cullin-associated NEDD8-dissociated protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012518.19, AC078983.17 and AA766567.1. On May 31, 2019 this sequence version replaced NM_001329674.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660809.168437.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01820689, SAMN03267750 [ECO:0000348] ##Evidence-Data-END##

ATGGCTACAA ATGATTTGAT GACGGAACTG CAGAAAGATT CCATCAAGTT GGATGATGAT 
AGTGAAAGGA AAGTAGTGAA AATGATTTTG AAGTTATTGG AAGATAAAAA TGGAGAGGTA 
CAGAATTTAG CTGTCAAATG TCTTGGTCCT TTAGTGAGTA AAGTGAAAGA ATACCAAGTA 
GAGACAATTG TAGATACCCT CTGCACTAAC ATGCTTTCTG ATAAAGAACA ACTTCGAGAC 
ATTTCAAGTA TTGGTCTTAA AACAGTAATT GGAGAACTTC CTCCAGCTTC CAGTGGCTCT 
GCATTAGCTG CTAATGTATG TAAAAAGATT ACTGGACGTC TTACAAGTGC AATAGCAAAA 
CAGGAAGATG TCTCTGTTCA GCTAGAAGCC TTGGATATTA TGGCTGATAT GTTGAGCAGG 
CAAGGAGGAC TTCTTGTTAA TTTCCATCCT TCAATTCTGA CCTGTCTACT TCCCCAGTTG 
ACCAGCCCTA GACTTGCAGT GAGGAAAAGA ACCATTATCG CTCTTGGCCA TCTGGTTATG 
AGCTGTGGAA ATATAGTTTT TGTAGATCTT ATTGAACATC TGTTGTCAGA GTTGTCCAAA 
AATGATTCTA TGTCAACAAC AAGAACCTAC ATACAATGTA TTGCTGCTAT TAGTAGGCAA 
GCTGGTCATA GAATAGGTGA ATACCTTGAG AAGATAATTC CTTTGGTGGT AAAATTTTGC 
AATGTAGATG ATGATGAATT AAGAGAGTAC TGTATTCAAG CCTTTGAATC ATTTGTAAGA 
AGATGTCCTA AGGAAGTATA TCCTCATGTT TCTACCATTA TAAATATTTG TCTTAAATAT 
CTTACCTATG ATCCAAATTA TAATTACGAT GATGAAGATG AAGATGAAAA TGCAATGGAT 
GCTGATGGTG GTGATGATGA TGATCAAGGG AGTGATGATG AATACAGTGA TGATGATGAC 
ATGAGTTGGA AAGTGAGACG TGCAGCTGCG AAGTGCTTGG ATGCTGTAGT TAGCACAAGG 
CATGAAATGC TTCCAGAATT CTACAAGACC GTCTCTCCTG CACTAATATC CAGATTTAAA 
GAGCGTGAAG AGAATGTAAA GGCAGATGTT TTTCACGCAT ACCTTTCTCT TTTGAAGCAA 
ACTCGTCCTG TACAAAGTTG GCTATGTGAC CCTGATGCAA TGGAGCAGGG AGAAACACCT 
TTAACAATGC TTCAGAGTCA GGTTCCCAAC ATTGTTAAAG CTCTTCACAA ACAGATGAAA 
GAAAAAAGTG TGAAGACCCG ACAGTGTTGT TTTAACATGT TAACTGAGCT GGTAAATGTA 
TTACCTGGGG CCCTAACTCA ACACATTCCT GTACTTGTAC CAGGAATCAT TTTCTCACTG 
AATGATAAAT CAAGCTCATC GAATTTGAAG ATCGATGCTT TGTCATGTCT ATACGTAATC 
CTCTGTAACC ATTCTCCTCA AGTCTTCCAT CCTCACGTTC AGGCTTTGGT TCCTCCAGTG 
GTGGCTTGTG TTGGAGACCC ATTTTACAAA ATTACATCTG AAGCACTTCT TGTTACTCAA 
CAGCTTGTCA AAGTAATTCG TCCTTTAGAT CAGCCTTCCT CGTTTGATGC AACTCCTTAT 
ATCAAAGATC TATTTACCTG TACCATTAAG AGATTAAAAG CAGCTGACAT TGATCAGGAA 
GTCAAGGAAA GGGCTATTTC CTGTATGGGA CAAATTATTT GCAACCTTGG AGACAATTTG 
GGTTCTGACT TGCCTAATAC ACTTCAGATT TTCTTGGAGA GACTAAAGAA TGAAATTACC 
AGGTTAACTA CAGTAAAGGC ATTGACACTG ATTGCTGGGT CACCTTTGAA GATAGATTTG 
AGGCCTGTTC TGGGAGAAGG GGTTCCTATC CTTGCTTCAT TTCTTAGAAA AAACCAGAGA 
GCTTTGAAAC TGGGTACTCT TTCTGCCCTT GATATTCTAA TAAAAAACTA TAGTGACAGC 
TTGACAGCTG CCATGATTGA TGCAGTTCTA GATGAGCTCC CACCTCTTAT CAGCGAAAGT 
GATATGCATG TTTCACAAAT GGCCATCAGT TTTCTTACCA CTTTGGCAAA AGTATATCCC 
TCCTCCCTTT CAAAGATAAG TGGATCCATT CTCAATGAAC TTATTGGACT TGTGAGATCA 
CCCTTATTGC AGGGGGGAGC TCTTAGTGCC ATGCTAGACT TTTTCCAAGC TCTGGTTGTC 
ACTGGAACAA ATAATTTAGG ATACATGGAT TTGTTGCGCA TGCTGACTGG TCCAGTTTAC 
TCTCAGAGCA CAGCTCTTAC TCATAAGCAG TCTTATTATT CCATTGCCAA ATGTGTAGCT 
GCCCTTACTC GAGCATGCCC TAAAGAGGGA CCAGCTGTAG TAGGTCAGTT TATTCAAGAT 
GTCAAGAACT CAAGGTCTAC AGATTCCATT CGTCTCTTAG CTCTACTTTC TCTTGGAGAA 
GTTGGGCATC ATATTGACTT AAGTGGACAG TTGGAACTAA AATCTGTAAT ACTAGAAGCT 
TTCTCATCTC CTAGTGAAGA AGTCAAATCA GCTGCATCCT ATGCATTAGG CAGCATTAGT 
GTGGGCAACC TTCCTGAATA TCTGCCGTTT GTCCTGCAAG AAATAACTAG TCAACCCAAA 
AGGCAGTATC TTTTACTTCA TTCCTTGAAG GAAATTATTA GCTCTGCATC AGTGGTGGGC 
CTTAAACCAT ATGTTGAAAA CATCTGGGCC TTATTACTAA AGCACTGTGA GTGTGCAGAG 
GAAGGAACCA GAAATGTTGT TGCTGAATGT CTAGGAAAAC TCACTCTAAT TGATCCAGAA 
ACTCTCCTTC CACGGCTTAA GGGGTACTTG ATATCAGGCT CATCATATGC CCGAAGCTCA 
GTGGTTACGG CTGTGAAATT TACAATTTCT GACCATCCAC AACCTATTGA TCCACTGTTA 
AAGAACTGCA TAGGTGATTT CCTAAAAACT TTGGAAGACC CAGATTTGAA TGTGAGAAGA 
GTAGCCTTGG TCACATTTAA TTCAGCAGCA CATAACAAGC CATCATTAAT AAGGGATCTA 
TTGGATACTG TTCTTCCACA TCTTTACAAT GAAACAAAAG TTAGAAAGGA GCTTATAAGA 
GAGGTAGAAA TGGGTCCATT TAAACATACG GTTGATGATG GTCTGGATAT TAGAAAGGCA 
GCATTTGAGT GTATGTACAC ACTTCTAGAC AGTTGTCTTG ATAGACTTGA TATCTTTGAA 
TTTCTAAATC ATGTTGAAGA TGGTTTGAAG GACCATTATG ATATTAAGAT GCTGACATTT 
TTAATGTTGG TGAGACTGTC TACCCTTTGT CCAAGTGCAG TACTGCAGAG GTTGGACCGA 
CTTGTTGAGC CATTACGTGC AACATGTACA ACTAAGGTAA AGGCAAACTC AGTAAAGCAG 
GAGTTTGAAA AACAAGATGA ATTAAAGCGA TCTGCCATGA GAGCAGTAGC AGCACTGCTA 
ACCATTCCAG AAGCAGAGAA GAGTCCACTG ATGAGTGAAT TCCAGTCACA GATCAGTTCT 
AACCCTGAGC TGGCGGCTAT CTTTGAAAGT ATCCAGAAAG ATTCATCATC TACTAACTTG 
GAATCAATGG ACACTAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316603.1
CDS	560..4180
Translation	MATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS

Target ORF information:

RefSeq Version	NM_001329674.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329674.2

ORF Insert Sequence:

ATGGCTACAA ATGATTTGAT GACGGAACTG CAGAAAGATT CCATCAAGTT GGATGATGAT 
AGTGAAAGGA AAGTAGTGAA AATGATTTTG AAGTTATTGG AAGATAAAAA TGGAGAGGTA 
CAGAATTTAG CTGTCAAATG TCTTGGTCCT TTAGTGAGTA AAGTGAAAGA ATACCAAGTA 
GAGACAATTG TAGATACCCT CTGCACTAAC ATGCTTTCTG ATAAAGAACA ACTTCGAGAC 
ATTTCAAGTA TTGGTCTTAA AACAGTAATT GGAGAACTTC CTCCAGCTTC CAGTGGCTCT 
GCATTAGCTG CTAATGTATG TAAAAAGATT ACTGGACGTC TTACAAGTGC AATAGCAAAA 
CAGGAAGATG TCTCTGTTCA GCTAGAAGCC TTGGATATTA TGGCTGATAT GTTGAGCAGG 
CAAGGAGGAC TTCTTGTTAA TTTCCATCCT TCAATTCTGA CCTGTCTACT TCCCCAGTTG 
ACCAGCCCTA GACTTGCAGT GAGGAAAAGA ACCATTATCG CTCTTGGCCA TCTGGTTATG 
AGCTGTGGAA ATATAGTTTT TGTAGATCTT ATTGAACATC TGTTGTCAGA GTTGTCCAAA 
AATGATTCTA TGTCAACAAC AAGAACCTAC ATACAATGTA TTGCTGCTAT TAGTAGGCAA 
GCTGGTCATA GAATAGGTGA ATACCTTGAG AAGATAATTC CTTTGGTGGT AAAATTTTGC 
AATGTAGATG ATGATGAATT AAGAGAGTAC TGTATTCAAG CCTTTGAATC ATTTGTAAGA 
AGATGTCCTA AGGAAGTATA TCCTCATGTT TCTACCATTA TAAATATTTG TCTTAAATAT 
CTTACCTATG ATCCAAATTA TAATTACGAT GATGAAGATG AAGATGAAAA TGCAATGGAT 
GCTGATGGTG GTGATGATGA TGATCAAGGG AGTGATGATG AATACAGTGA TGATGATGAC 
ATGAGTTGGA AAGTGAGACG TGCAGCTGCG AAGTGCTTGG ATGCTGTAGT TAGCACAAGG 
CATGAAATGC TTCCAGAATT CTACAAGACC GTCTCTCCTG CACTAATATC CAGATTTAAA 
GAGCGTGAAG AGAATGTAAA GGCAGATGTT TTTCACGCAT ACCTTTCTCT TTTGAAGCAA 
ACTCGTCCTG TACAAAGTTG GCTATGTGAC CCTGATGCAA TGGAGCAGGG AGAAACACCT 
TTAACAATGC TTCAGAGTCA GGTTCCCAAC ATTGTTAAAG CTCTTCACAA ACAGATGAAA 
GAAAAAAGTG TGAAGACCCG ACAGTGTTGT TTTAACATGT TAACTGAGCT GGTAAATGTA 
TTACCTGGGG CCCTAACTCA ACACATTCCT GTACTTGTAC CAGGAATCAT TTTCTCACTG 
AATGATAAAT CAAGCTCATC GAATTTGAAG ATCGATGCTT TGTCATGTCT ATACGTAATC 
CTCTGTAACC ATTCTCCTCA AGTCTTCCAT CCTCACGTTC AGGCTTTGGT TCCTCCAGTG 
GTGGCTTGTG TTGGAGACCC ATTTTACAAA ATTACATCTG AAGCACTTCT TGTTACTCAA 
CAGCTTGTCA AAGTAATTCG TCCTTTAGAT CAGCCTTCCT CGTTTGATGC AACTCCTTAT 
ATCAAAGATC TATTTACCTG TACCATTAAG AGATTAAAAG CAGCTGACAT TGATCAGGAA 
GTCAAGGAAA GGGCTATTTC CTGTATGGGA CAAATTATTT GCAACCTTGG AGACAATTTG 
GGTTCTGACT TGCCTAATAC ACTTCAGATT TTCTTGGAGA GACTAAAGAA TGAAATTACC 
AGGTTAACTA CAGTAAAGGC ATTGACACTG ATTGCTGGGT CACCTTTGAA GATAGATTTG 
AGGCCTGTTC TGGGAGAAGG GGTTCCTATC CTTGCTTCAT TTCTTAGAAA AAACCAGAGA 
GCTTTGAAAC TGGGTACTCT TTCTGCCCTT GATATTCTAA TAAAAAACTA TAGTGACAGC 
TTGACAGCTG CCATGATTGA TGCAGTTCTA GATGAGCTCC CACCTCTTAT CAGCGAAAGT 
GATATGCATG TTTCACAAAT GGCCATCAGT TTTCTTACCA CTTTGGCAAA AGTATATCCC 
TCCTCCCTTT CAAAGATAAG TGGATCCATT CTCAATGAAC TTATTGGACT TGTGAGATCA 
CCCTTATTGC AGGGGGGAGC TCTTAGTGCC ATGCTAGACT TTTTCCAAGC TCTGGTTGTC 
ACTGGAACAA ATAATTTAGG ATACATGGAT TTGTTGCGCA TGCTGACTGG TCCAGTTTAC 
TCTCAGAGCA CAGCTCTTAC TCATAAGCAG TCTTATTATT CCATTGCCAA ATGTGTAGCT 
GCCCTTACTC GAGCATGCCC TAAAGAGGGA CCAGCTGTAG TAGGTCAGTT TATTCAAGAT 
GTCAAGAACT CAAGGTCTAC AGATTCCATT CGTCTCTTAG CTCTACTTTC TCTTGGAGAA 
GTTGGGCATC ATATTGACTT AAGTGGACAG TTGGAACTAA AATCTGTAAT ACTAGAAGCT 
TTCTCATCTC CTAGTGAAGA AGTCAAATCA GCTGCATCCT ATGCATTAGG CAGCATTAGT 
GTGGGCAACC TTCCTGAATA TCTGCCGTTT GTCCTGCAAG AAATAACTAG TCAACCCAAA 
AGGCAGTATC TTTTACTTCA TTCCTTGAAG GAAATTATTA GCTCTGCATC AGTGGTGGGC 
CTTAAACCAT ATGTTGAAAA CATCTGGGCC TTATTACTAA AGCACTGTGA GTGTGCAGAG 
GAAGGAACCA GAAATGTTGT TGCTGAATGT CTAGGAAAAC TCACTCTAAT TGATCCAGAA 
ACTCTCCTTC CACGGCTTAA GGGGTACTTG ATATCAGGCT CATCATATGC CCGAAGCTCA 
GTGGTTACGG CTGTGAAATT TACAATTTCT GACCATCCAC AACCTATTGA TCCACTGTTA 
AAGAACTGCA TAGGTGATTT CCTAAAAACT TTGGAAGACC CAGATTTGAA TGTGAGAAGA 
GTAGCCTTGG TCACATTTAA TTCAGCAGCA CATAACAAGC CATCATTAAT AAGGGATCTA 
TTGGATACTG TTCTTCCACA TCTTTACAAT GAAACAAAAG TTAGAAAGGA GCTTATAAGA 
GAGGTAGAAA TGGGTCCATT TAAACATACG GTTGATGATG GTCTGGATAT TAGAAAGGCA 
GCATTTGAGT GTATGTACAC ACTTCTAGAC AGTTGTCTTG ATAGACTTGA TATCTTTGAA 
TTTCTAAATC ATGTTGAAGA TGGTTTGAAG GACCATTATG ATATTAAGAT GCTGACATTT 
TTAATGTTGG TGAGACTGTC TACCCTTTGT CCAAGTGCAG TACTGCAGAG GTTGGACCGA 
CTTGTTGAGC CATTACGTGC AACATGTACA ACTAAGGTAA AGGCAAACTC AGTAAAGCAG 
GAGTTTGAAA AACAAGATGA ATTAAAGCGA TCTGCCATGA GAGCAGTAGC AGCACTGCTA 
ACCATTCCAG AAGCAGAGAA GAGTCCACTG ATGAGTGAAT TCCAGTCACA GATCAGTTCT 
AACCCTGAGC TGGCGGCTAT CTTTGAAAGT ATCCAGAAAG ATTCATCATC TACTAACTTG 
GAATCAATGG ACACTAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAND1 ( NM_001329675.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316604.1 Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 3, mRNA.

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CloneID	OHu101665
Clone ID Related Accession (Same CDS sequence)	NM_001329674.1 , NM_001329675.1 , NM_001329674.2 , NM_001329675.2
Accession Version	NM_001329675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	cullin-associated NEDD8-dissociated protein 1 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012518.19, AC078983.17 and AA766567.1. On May 31, 2019 this sequence version replaced NM_001329675.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803611.89650.1 [ECO:0000332] ##Evidence-Data-END##

ATGGCTACAA ATGATTTGAT GACGGAACTG CAGAAAGATT CCATCAAGTT GGATGATGAT 
AGTGAAAGGA AAGTAGTGAA AATGATTTTG AAGTTATTGG AAGATAAAAA TGGAGAGGTA 
CAGAATTTAG CTGTCAAATG TCTTGGTCCT TTAGTGAGTA AAGTGAAAGA ATACCAAGTA 
GAGACAATTG TAGATACCCT CTGCACTAAC ATGCTTTCTG ATAAAGAACA ACTTCGAGAC 
ATTTCAAGTA TTGGTCTTAA AACAGTAATT GGAGAACTTC CTCCAGCTTC CAGTGGCTCT 
GCATTAGCTG CTAATGTATG TAAAAAGATT ACTGGACGTC TTACAAGTGC AATAGCAAAA 
CAGGAAGATG TCTCTGTTCA GCTAGAAGCC TTGGATATTA TGGCTGATAT GTTGAGCAGG 
CAAGGAGGAC TTCTTGTTAA TTTCCATCCT TCAATTCTGA CCTGTCTACT TCCCCAGTTG 
ACCAGCCCTA GACTTGCAGT GAGGAAAAGA ACCATTATCG CTCTTGGCCA TCTGGTTATG 
AGCTGTGGAA ATATAGTTTT TGTAGATCTT ATTGAACATC TGTTGTCAGA GTTGTCCAAA 
AATGATTCTA TGTCAACAAC AAGAACCTAC ATACAATGTA TTGCTGCTAT TAGTAGGCAA 
GCTGGTCATA GAATAGGTGA ATACCTTGAG AAGATAATTC CTTTGGTGGT AAAATTTTGC 
AATGTAGATG ATGATGAATT AAGAGAGTAC TGTATTCAAG CCTTTGAATC ATTTGTAAGA 
AGATGTCCTA AGGAAGTATA TCCTCATGTT TCTACCATTA TAAATATTTG TCTTAAATAT 
CTTACCTATG ATCCAAATTA TAATTACGAT GATGAAGATG AAGATGAAAA TGCAATGGAT 
GCTGATGGTG GTGATGATGA TGATCAAGGG AGTGATGATG AATACAGTGA TGATGATGAC 
ATGAGTTGGA AAGTGAGACG TGCAGCTGCG AAGTGCTTGG ATGCTGTAGT TAGCACAAGG 
CATGAAATGC TTCCAGAATT CTACAAGACC GTCTCTCCTG CACTAATATC CAGATTTAAA 
GAGCGTGAAG AGAATGTAAA GGCAGATGTT TTTCACGCAT ACCTTTCTCT TTTGAAGCAA 
ACTCGTCCTG TACAAAGTTG GCTATGTGAC CCTGATGCAA TGGAGCAGGG AGAAACACCT 
TTAACAATGC TTCAGAGTCA GGTTCCCAAC ATTGTTAAAG CTCTTCACAA ACAGATGAAA 
GAAAAAAGTG TGAAGACCCG ACAGTGTTGT TTTAACATGT TAACTGAGCT GGTAAATGTA 
TTACCTGGGG CCCTAACTCA ACACATTCCT GTACTTGTAC CAGGAATCAT TTTCTCACTG 
AATGATAAAT CAAGCTCATC GAATTTGAAG ATCGATGCTT TGTCATGTCT ATACGTAATC 
CTCTGTAACC ATTCTCCTCA AGTCTTCCAT CCTCACGTTC AGGCTTTGGT TCCTCCAGTG 
GTGGCTTGTG TTGGAGACCC ATTTTACAAA ATTACATCTG AAGCACTTCT TGTTACTCAA 
CAGCTTGTCA AAGTAATTCG TCCTTTAGAT CAGCCTTCCT CGTTTGATGC AACTCCTTAT 
ATCAAAGATC TATTTACCTG TACCATTAAG AGATTAAAAG CAGCTGACAT TGATCAGGAA 
GTCAAGGAAA GGGCTATTTC CTGTATGGGA CAAATTATTT GCAACCTTGG AGACAATTTG 
GGTTCTGACT TGCCTAATAC ACTTCAGATT TTCTTGGAGA GACTAAAGAA TGAAATTACC 
AGGTTAACTA CAGTAAAGGC ATTGACACTG ATTGCTGGGT CACCTTTGAA GATAGATTTG 
AGGCCTGTTC TGGGAGAAGG GGTTCCTATC CTTGCTTCAT TTCTTAGAAA AAACCAGAGA 
GCTTTGAAAC TGGGTACTCT TTCTGCCCTT GATATTCTAA TAAAAAACTA TAGTGACAGC 
TTGACAGCTG CCATGATTGA TGCAGTTCTA GATGAGCTCC CACCTCTTAT CAGCGAAAGT 
GATATGCATG TTTCACAAAT GGCCATCAGT TTTCTTACCA CTTTGGCAAA AGTATATCCC 
TCCTCCCTTT CAAAGATAAG TGGATCCATT CTCAATGAAC TTATTGGACT TGTGAGATCA 
CCCTTATTGC AGGGGGGAGC TCTTAGTGCC ATGCTAGACT TTTTCCAAGC TCTGGTTGTC 
ACTGGAACAA ATAATTTAGG ATACATGGAT TTGTTGCGCA TGCTGACTGG TCCAGTTTAC 
TCTCAGAGCA CAGCTCTTAC TCATAAGCAG TCTTATTATT CCATTGCCAA ATGTGTAGCT 
GCCCTTACTC GAGCATGCCC TAAAGAGGGA CCAGCTGTAG TAGGTCAGTT TATTCAAGAT 
GTCAAGAACT CAAGGTCTAC AGATTCCATT CGTCTCTTAG CTCTACTTTC TCTTGGAGAA 
GTTGGGCATC ATATTGACTT AAGTGGACAG TTGGAACTAA AATCTGTAAT ACTAGAAGCT 
TTCTCATCTC CTAGTGAAGA AGTCAAATCA GCTGCATCCT ATGCATTAGG CAGCATTAGT 
GTGGGCAACC TTCCTGAATA TCTGCCGTTT GTCCTGCAAG AAATAACTAG TCAACCCAAA 
AGGCAGTATC TTTTACTTCA TTCCTTGAAG GAAATTATTA GCTCTGCATC AGTGGTGGGC 
CTTAAACCAT ATGTTGAAAA CATCTGGGCC TTATTACTAA AGCACTGTGA GTGTGCAGAG 
GAAGGAACCA GAAATGTTGT TGCTGAATGT CTAGGAAAAC TCACTCTAAT TGATCCAGAA 
ACTCTCCTTC CACGGCTTAA GGGGTACTTG ATATCAGGCT CATCATATGC CCGAAGCTCA 
GTGGTTACGG CTGTGAAATT TACAATTTCT GACCATCCAC AACCTATTGA TCCACTGTTA 
AAGAACTGCA TAGGTGATTT CCTAAAAACT TTGGAAGACC CAGATTTGAA TGTGAGAAGA 
GTAGCCTTGG TCACATTTAA TTCAGCAGCA CATAACAAGC CATCATTAAT AAGGGATCTA 
TTGGATACTG TTCTTCCACA TCTTTACAAT GAAACAAAAG TTAGAAAGGA GCTTATAAGA 
GAGGTAGAAA TGGGTCCATT TAAACATACG GTTGATGATG GTCTGGATAT TAGAAAGGCA 
GCATTTGAGT GTATGTACAC ACTTCTAGAC AGTTGTCTTG ATAGACTTGA TATCTTTGAA 
TTTCTAAATC ATGTTGAAGA TGGTTTGAAG GACCATTATG ATATTAAGAT GCTGACATTT 
TTAATGTTGG TGAGACTGTC TACCCTTTGT CCAAGTGCAG TACTGCAGAG GTTGGACCGA 
CTTGTTGAGC CATTACGTGC AACATGTACA ACTAAGGTAA AGGCAAACTC AGTAAAGCAG 
GAGTTTGAAA AACAAGATGA ATTAAAGCGA TCTGCCATGA GAGCAGTAGC AGCACTGCTA 
ACCATTCCAG AAGCAGAGAA GAGTCCACTG ATGAGTGAAT TCCAGTCACA GATCAGTTCT 
AACCCTGAGC TGGCGGCTAT CTTTGAAAGT ATCCAGAAAG ATTCATCATC TACTAACTTG 
GAATCAATGG ACACTAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316604.1
CDS	554..4174
Translation	MATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS

Target ORF information:

RefSeq Version	NM_001329675.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329675.2

ORF Insert Sequence:

ATGGCTACAA ATGATTTGAT GACGGAACTG CAGAAAGATT CCATCAAGTT GGATGATGAT 
AGTGAAAGGA AAGTAGTGAA AATGATTTTG AAGTTATTGG AAGATAAAAA TGGAGAGGTA 
CAGAATTTAG CTGTCAAATG TCTTGGTCCT TTAGTGAGTA AAGTGAAAGA ATACCAAGTA 
GAGACAATTG TAGATACCCT CTGCACTAAC ATGCTTTCTG ATAAAGAACA ACTTCGAGAC 
ATTTCAAGTA TTGGTCTTAA AACAGTAATT GGAGAACTTC CTCCAGCTTC CAGTGGCTCT 
GCATTAGCTG CTAATGTATG TAAAAAGATT ACTGGACGTC TTACAAGTGC AATAGCAAAA 
CAGGAAGATG TCTCTGTTCA GCTAGAAGCC TTGGATATTA TGGCTGATAT GTTGAGCAGG 
CAAGGAGGAC TTCTTGTTAA TTTCCATCCT TCAATTCTGA CCTGTCTACT TCCCCAGTTG 
ACCAGCCCTA GACTTGCAGT GAGGAAAAGA ACCATTATCG CTCTTGGCCA TCTGGTTATG 
AGCTGTGGAA ATATAGTTTT TGTAGATCTT ATTGAACATC TGTTGTCAGA GTTGTCCAAA 
AATGATTCTA TGTCAACAAC AAGAACCTAC ATACAATGTA TTGCTGCTAT TAGTAGGCAA 
GCTGGTCATA GAATAGGTGA ATACCTTGAG AAGATAATTC CTTTGGTGGT AAAATTTTGC 
AATGTAGATG ATGATGAATT AAGAGAGTAC TGTATTCAAG CCTTTGAATC ATTTGTAAGA 
AGATGTCCTA AGGAAGTATA TCCTCATGTT TCTACCATTA TAAATATTTG TCTTAAATAT 
CTTACCTATG ATCCAAATTA TAATTACGAT GATGAAGATG AAGATGAAAA TGCAATGGAT 
GCTGATGGTG GTGATGATGA TGATCAAGGG AGTGATGATG AATACAGTGA TGATGATGAC 
ATGAGTTGGA AAGTGAGACG TGCAGCTGCG AAGTGCTTGG ATGCTGTAGT TAGCACAAGG 
CATGAAATGC TTCCAGAATT CTACAAGACC GTCTCTCCTG CACTAATATC CAGATTTAAA 
GAGCGTGAAG AGAATGTAAA GGCAGATGTT TTTCACGCAT ACCTTTCTCT TTTGAAGCAA 
ACTCGTCCTG TACAAAGTTG GCTATGTGAC CCTGATGCAA TGGAGCAGGG AGAAACACCT 
TTAACAATGC TTCAGAGTCA GGTTCCCAAC ATTGTTAAAG CTCTTCACAA ACAGATGAAA 
GAAAAAAGTG TGAAGACCCG ACAGTGTTGT TTTAACATGT TAACTGAGCT GGTAAATGTA 
TTACCTGGGG CCCTAACTCA ACACATTCCT GTACTTGTAC CAGGAATCAT TTTCTCACTG 
AATGATAAAT CAAGCTCATC GAATTTGAAG ATCGATGCTT TGTCATGTCT ATACGTAATC 
CTCTGTAACC ATTCTCCTCA AGTCTTCCAT CCTCACGTTC AGGCTTTGGT TCCTCCAGTG 
GTGGCTTGTG TTGGAGACCC ATTTTACAAA ATTACATCTG AAGCACTTCT TGTTACTCAA 
CAGCTTGTCA AAGTAATTCG TCCTTTAGAT CAGCCTTCCT CGTTTGATGC AACTCCTTAT 
ATCAAAGATC TATTTACCTG TACCATTAAG AGATTAAAAG CAGCTGACAT TGATCAGGAA 
GTCAAGGAAA GGGCTATTTC CTGTATGGGA CAAATTATTT GCAACCTTGG AGACAATTTG 
GGTTCTGACT TGCCTAATAC ACTTCAGATT TTCTTGGAGA GACTAAAGAA TGAAATTACC 
AGGTTAACTA CAGTAAAGGC ATTGACACTG ATTGCTGGGT CACCTTTGAA GATAGATTTG 
AGGCCTGTTC TGGGAGAAGG GGTTCCTATC CTTGCTTCAT TTCTTAGAAA AAACCAGAGA 
GCTTTGAAAC TGGGTACTCT TTCTGCCCTT GATATTCTAA TAAAAAACTA TAGTGACAGC 
TTGACAGCTG CCATGATTGA TGCAGTTCTA GATGAGCTCC CACCTCTTAT CAGCGAAAGT 
GATATGCATG TTTCACAAAT GGCCATCAGT TTTCTTACCA CTTTGGCAAA AGTATATCCC 
TCCTCCCTTT CAAAGATAAG TGGATCCATT CTCAATGAAC TTATTGGACT TGTGAGATCA 
CCCTTATTGC AGGGGGGAGC TCTTAGTGCC ATGCTAGACT TTTTCCAAGC TCTGGTTGTC 
ACTGGAACAA ATAATTTAGG ATACATGGAT TTGTTGCGCA TGCTGACTGG TCCAGTTTAC 
TCTCAGAGCA CAGCTCTTAC TCATAAGCAG TCTTATTATT CCATTGCCAA ATGTGTAGCT 
GCCCTTACTC GAGCATGCCC TAAAGAGGGA CCAGCTGTAG TAGGTCAGTT TATTCAAGAT 
GTCAAGAACT CAAGGTCTAC AGATTCCATT CGTCTCTTAG CTCTACTTTC TCTTGGAGAA 
GTTGGGCATC ATATTGACTT AAGTGGACAG TTGGAACTAA AATCTGTAAT ACTAGAAGCT 
TTCTCATCTC CTAGTGAAGA AGTCAAATCA GCTGCATCCT ATGCATTAGG CAGCATTAGT 
GTGGGCAACC TTCCTGAATA TCTGCCGTTT GTCCTGCAAG AAATAACTAG TCAACCCAAA 
AGGCAGTATC TTTTACTTCA TTCCTTGAAG GAAATTATTA GCTCTGCATC AGTGGTGGGC 
CTTAAACCAT ATGTTGAAAA CATCTGGGCC TTATTACTAA AGCACTGTGA GTGTGCAGAG 
GAAGGAACCA GAAATGTTGT TGCTGAATGT CTAGGAAAAC TCACTCTAAT TGATCCAGAA 
ACTCTCCTTC CACGGCTTAA GGGGTACTTG ATATCAGGCT CATCATATGC CCGAAGCTCA 
GTGGTTACGG CTGTGAAATT TACAATTTCT GACCATCCAC AACCTATTGA TCCACTGTTA 
AAGAACTGCA TAGGTGATTT CCTAAAAACT TTGGAAGACC CAGATTTGAA TGTGAGAAGA 
GTAGCCTTGG TCACATTTAA TTCAGCAGCA CATAACAAGC CATCATTAAT AAGGGATCTA 
TTGGATACTG TTCTTCCACA TCTTTACAAT GAAACAAAAG TTAGAAAGGA GCTTATAAGA 
GAGGTAGAAA TGGGTCCATT TAAACATACG GTTGATGATG GTCTGGATAT TAGAAAGGCA 
GCATTTGAGT GTATGTACAC ACTTCTAGAC AGTTGTCTTG ATAGACTTGA TATCTTTGAA 
TTTCTAAATC ATGTTGAAGA TGGTTTGAAG GACCATTATG ATATTAAGAT GCTGACATTT 
TTAATGTTGG TGAGACTGTC TACCCTTTGT CCAAGTGCAG TACTGCAGAG GTTGGACCGA 
CTTGTTGAGC CATTACGTGC AACATGTACA ACTAAGGTAA AGGCAAACTC AGTAAAGCAG 
GAGTTTGAAA AACAAGATGA ATTAAAGCGA TCTGCCATGA GAGCAGTAGC AGCACTGCTA 
ACCATTCCAG AAGCAGAGAA GAGTCCACTG ATGAGTGAAT TCCAGTCACA GATCAGTTCT 
AACCCTGAGC TGGCGGCTAT CTTTGAAAGT ATCCAGAAAG ATTCATCATC TACTAACTTG 
GAATCAATGG ACACTAGTTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAND1 ( NM_001329676.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316605.1 Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 4, mRNA.

Price & Availability↑

CloneID	OHu101809
Clone ID Related Accession (Same CDS sequence)	NM_001329676.2 , NM_001329676.1
Accession Version	NM_001329676.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-27
Organism	Homo sapiens(human)
Product	cullin-associated NEDD8-dissociated protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012518.19, AC078983.17 and AA766567.1. On May 31, 2019 this sequence version replaced NM_001329676.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.93076.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGATTTTGG AATATTTGCA TATACAAAAT GAGATAACTT GGGGATGGAA CCCAAGTCTA 
AACATGAAAT TAATTTATGT TTCATATATA TCTCTTATAC ACATAGCCCG AAGTCTTGGT 
CCTTTAGTGA GTAAAGTGAA AGAATACCAA GTAGAGACAA TTGTAGATAC CCTCTGCACT 
AACATGCTTT CTGATAAAGA ACAACTTCGA GACATTTCAA GTATTGGTCT TAAAACAGTA 
ATTGGAGAAC TTCCTCCAGC TTCCAGTGGC TCTGCATTAG CTGCTAATGT ATGTAAAAAG 
ATTACTGGAC GTCTTACAAG TGCAATAGCA AAACAGGAAG ATGTCTCTGT TCAGCTAGAA 
GCCTTGGATA TTATGGCTGA TATGTTGAGC AGGCAAGGAG GACTTCTTGT TAATTTCCAT 
CCTTCAATTC TGACCTGTCT ACTTCCCCAG TTGACCAGCC CTAGACTTGC AGTGAGGAAA 
AGAACCATTA TCGCTCTTGG CCATCTGGTT ATGAGCTGTG GAAATATAGT TTTTGTAGAT 
CTTATTGAAC ATCTGTTGTC AGAGTTGTCC AAAAATGATT CTATGTCAAC AACAAGAACC 
TACATACAAT GTATTGCTGC TATTAGTAGG CAAGCTGGTC ATAGAATAGG TGAATACCTT 
GAGAAGATAA TTCCTTTGGT GGTAAAATTT TGCAATGTAG ATGATGATGA ATTAAGAGAG 
TACTGTATTC AAGCCTTTGA ATCATTTGTA AGAAGATGTC CTAAGGAAGT ATATCCTCAT 
GTTTCTACCA TTATAAATAT TTGTCTTAAA TATCTTACCT ATGATCCAAA TTATAATTAC 
GATGATGAAG ATGAAGATGA AAATGCAATG GATGCTGATG GTGGTGATGA TGATGATCAA 
GGGAGTGATG ATGAATACAG TGATGATGAT GACATGAGTT GGAAAGTGAG ACGTGCAGCT 
GCGAAGTGCT TGGATGCTGT AGTTAGCACA AGGCATGAAA TGCTTCCAGA ATTCTACAAG 
ACCGTCTCTC CTGCACTAAT ATCCAGATTT AAAGAGCGTG AAGAGAATGT AAAGGCAGAT 
GTTTTTCACG CATACCTTTC TCTTTTGAAG CAAACTCGTC CTGTACAAAG TTGGCTATGT 
GACCCTGATG CAATGGAGCA GGGAGAAACA CCTTTAACAA TGCTTCAGAG TCAGGTTCCC 
AACATTGTTA AAGCTCTTCA CAAACAGATG AAAGAAAAAA GTGTGAAGAC CCGACAGTGT 
TGTTTTAACA TGTTAACTGA GCTGGTAAAT GTATTACCTG GGGCCCTAAC TCAACACATT 
CCTGTACTTG TACCAGGAAT CATTTTCTCA CTGAATGATA AATCAAGCTC ATCGAATTTG 
AAGATCGATG CTTTGTCATG TCTATACGTA ATCCTCTGTA ACCATTCTCC TCAAGTCTTC 
CATCCTCACG TTCAGGCTTT GGTTCCTCCA GTGGTGGCTT GTGTTGGAGA CCCATTTTAC 
AAAATTACAT CTGAAGCACT TCTTGTTACT CAACAGCTTG TCAAAGTAAT TCGTCCTTTA 
GATCAGCCTT CCTCGTTTGA TGCAACTCCT TATATCAAAG ATCTATTTAC CTGTACCATT 
AAGAGATTAA AAGCAGCTGA CATTGATCAG GAAGTCAAGG AAAGGGCTAT TTCCTGTATG 
GGACAAATTA TTTGCAACCT TGGAGACAAT TTGGGTTCTG ACTTGCCTAA TACACTTCAG 
ATTTTCTTGG AGAGACTAAA GAATGAAATT ACCAGGTTAA CTACAGTAAA GGCATTGACA 
CTGATTGCTG GGTCACCTTT GAAGATAGAT TTGAGGCCTG TTCTGGGAGA AGGGGTTCCT 
ATCCTTGCTT CATTTCTTAG AAAAAACCAG AGAGCTTTGA AACTGGGTAC TCTTTCTGCC 
CTTGATATTC TAATAAAAAA CTATAGTGAC AGCTTGACAG CTGCCATGAT TGATGCAGTT 
CTAGATGAGC TCCCACCTCT TATCAGCGAA AGTGATATGC ATGTTTCACA AATGGCCATC 
AGTTTTCTTA CCACTTTGGC AAAAGTATAT CCCTCCTCCC TTTCAAAGAT AAGTGGATCC 
ATTCTCAATG AACTTATTGG ACTTGTGAGA TCACCCTTAT TGCAGGGGGG AGCTCTTAGT 
GCCATGCTAG ACTTTTTCCA AGCTCTGGTT GTCACTGGAA CAAATAATTT AGGATACATG 
GATTTGTTGC GCATGCTGAC TGGTCCAGTT TACTCTCAGA GCACAGCTCT TACTCATAAG 
CAGTCTTATT ATTCCATTGC CAAATGTGTA GCTGCCCTTA CTCGAGCATG CCCTAAAGAG 
GGACCAGCTG TAGTAGGTCA GTTTATTCAA GATGTCAAGA ACTCAAGGTC TACAGATTCC 
ATTCGTCTCT TAGCTCTACT TTCTCTTGGA GAAGTTGGGC ATCATATTGA CTTAAGTGGA 
CAGTTGGAAC TAAAATCTGT AATACTAGAA GCTTTCTCAT CTCCTAGTGA AGAAGTCAAA 
TCAGCTGCAT CCTATGCATT AGGCAGCATT AGTGTGGGCA ACCTTCCTGA ATATCTGCCG 
TTTGTCCTGC AAGAAATAAC TAGTCAACCC AAAAGGCAGT ATCTTTTACT TCATTCCTTG 
AAGGAAATTA TTAGCTCTGC ATCAGTGGTG GGCCTTAAAC CATATGTTGA AAACATCTGG 
GCCTTATTAC TAAAGCACTG TGAGTGTGCA GAGGAAGGAA CCAGAAATGT TGTTGCTGAA 
TGTCTAGGAA AACTCACTCT AATTGATCCA GAAACTCTCC TTCCACGGCT TAAGGGGTAC 
TTGATATCAG GCTCATCATA TGCCCGAAGC TCAGTGGTTA CGGCTGTGAA ATTTACAATT 
TCTGACCATC CACAACCTAT TGATCCACTG TTAAAGAACT GCATAGGTGA TTTCCTAAAA 
ACTTTGGAAG ACCCAGATTT GAATGTGAGA AGAGTAGCCT TGGTCACATT TAATTCAGCA 
GCACATAACA AGCCATCATT AATAAGGGAT CTATTGGATA CTGTTCTTCC ACATCTTTAC 
AATGAAACAA AAGTTAGAAA GGAGCTTATA AGAGAGGTAG AAATGGGTCC ATTTAAACAT 
ACGGTTGATG ATGGTCTGGA TATTAGAAAG GCAGCATTTG AGTGTATGTA CACACTTCTA 
GACAGTTGTC TTGATAGACT TGATATCTTT GAATTTCTAA ATCATGTTGA AGATGGTTTG 
AAGGACCATT ATGATATTAA GATGCTGACA TTTTTAATGT TGGTGAGACT GTCTACCCTT 
TGTCCAAGTG CAGTACTGCA GAGGTTGGAC CGACTTGTTG AGCCATTACG TGCAACATGT 
ACAACTAAGG TAAAGGCAAA CTCAGTAAAG CAGGAGTTTG AAAAACAAGA TGAATTAAAG 
CGATCTGCCA TGAGAGCAGT AGCAGCACTG CTAACCATTC CAGAAGCAGA GAAGAGTCCA 
CTGATGAGTG AATTCCAGTC ACAGATCAGT TCTAACCCTG AGCTGGCGGC TATCTTTGAA 
AGTATCCAGA AAGATTCATC ATCTACTAAC TTGGAATCAA TGGACACTAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316605.1
CDS	570..4163
Translation	MILEYLHIQNEITWGWNPSLNMKLIYVSYISLIHIARSLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS

Target ORF information:

RefSeq Version	NM_001329676.2
Organism	Homo sapiens(human)
Definition	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329676.2

ORF Insert Sequence:

ATGATTTTGG AATATTTGCA TATACAAAAT GAGATAACTT GGGGATGGAA CCCAAGTCTA 
AACATGAAAT TAATTTATGT TTCATATATA TCTCTTATAC ACATAGCCCG AAGTCTTGGT 
CCTTTAGTGA GTAAAGTGAA AGAATACCAA GTAGAGACAA TTGTAGATAC CCTCTGCACT 
AACATGCTTT CTGATAAAGA ACAACTTCGA GACATTTCAA GTATTGGTCT TAAAACAGTA 
ATTGGAGAAC TTCCTCCAGC TTCCAGTGGC TCTGCATTAG CTGCTAATGT ATGTAAAAAG 
ATTACTGGAC GTCTTACAAG TGCAATAGCA AAACAGGAAG ATGTCTCTGT TCAGCTAGAA 
GCCTTGGATA TTATGGCTGA TATGTTGAGC AGGCAAGGAG GACTTCTTGT TAATTTCCAT 
CCTTCAATTC TGACCTGTCT ACTTCCCCAG TTGACCAGCC CTAGACTTGC AGTGAGGAAA 
AGAACCATTA TCGCTCTTGG CCATCTGGTT ATGAGCTGTG GAAATATAGT TTTTGTAGAT 
CTTATTGAAC ATCTGTTGTC AGAGTTGTCC AAAAATGATT CTATGTCAAC AACAAGAACC 
TACATACAAT GTATTGCTGC TATTAGTAGG CAAGCTGGTC ATAGAATAGG TGAATACCTT 
GAGAAGATAA TTCCTTTGGT GGTAAAATTT TGCAATGTAG ATGATGATGA ATTAAGAGAG 
TACTGTATTC AAGCCTTTGA ATCATTTGTA AGAAGATGTC CTAAGGAAGT ATATCCTCAT 
GTTTCTACCA TTATAAATAT TTGTCTTAAA TATCTTACCT ATGATCCAAA TTATAATTAC 
GATGATGAAG ATGAAGATGA AAATGCAATG GATGCTGATG GTGGTGATGA TGATGATCAA 
GGGAGTGATG ATGAATACAG TGATGATGAT GACATGAGTT GGAAAGTGAG ACGTGCAGCT 
GCGAAGTGCT TGGATGCTGT AGTTAGCACA AGGCATGAAA TGCTTCCAGA ATTCTACAAG 
ACCGTCTCTC CTGCACTAAT ATCCAGATTT AAAGAGCGTG AAGAGAATGT AAAGGCAGAT 
GTTTTTCACG CATACCTTTC TCTTTTGAAG CAAACTCGTC CTGTACAAAG TTGGCTATGT 
GACCCTGATG CAATGGAGCA GGGAGAAACA CCTTTAACAA TGCTTCAGAG TCAGGTTCCC 
AACATTGTTA AAGCTCTTCA CAAACAGATG AAAGAAAAAA GTGTGAAGAC CCGACAGTGT 
TGTTTTAACA TGTTAACTGA GCTGGTAAAT GTATTACCTG GGGCCCTAAC TCAACACATT 
CCTGTACTTG TACCAGGAAT CATTTTCTCA CTGAATGATA AATCAAGCTC ATCGAATTTG 
AAGATCGATG CTTTGTCATG TCTATACGTA ATCCTCTGTA ACCATTCTCC TCAAGTCTTC 
CATCCTCACG TTCAGGCTTT GGTTCCTCCA GTGGTGGCTT GTGTTGGAGA CCCATTTTAC 
AAAATTACAT CTGAAGCACT TCTTGTTACT CAACAGCTTG TCAAAGTAAT TCGTCCTTTA 
GATCAGCCTT CCTCGTTTGA TGCAACTCCT TATATCAAAG ATCTATTTAC CTGTACCATT 
AAGAGATTAA AAGCAGCTGA CATTGATCAG GAAGTCAAGG AAAGGGCTAT TTCCTGTATG 
GGACAAATTA TTTGCAACCT TGGAGACAAT TTGGGTTCTG ACTTGCCTAA TACACTTCAG 
ATTTTCTTGG AGAGACTAAA GAATGAAATT ACCAGGTTAA CTACAGTAAA GGCATTGACA 
CTGATTGCTG GGTCACCTTT GAAGATAGAT TTGAGGCCTG TTCTGGGAGA AGGGGTTCCT 
ATCCTTGCTT CATTTCTTAG AAAAAACCAG AGAGCTTTGA AACTGGGTAC TCTTTCTGCC 
CTTGATATTC TAATAAAAAA CTATAGTGAC AGCTTGACAG CTGCCATGAT TGATGCAGTT 
CTAGATGAGC TCCCACCTCT TATCAGCGAA AGTGATATGC ATGTTTCACA AATGGCCATC 
AGTTTTCTTA CCACTTTGGC AAAAGTATAT CCCTCCTCCC TTTCAAAGAT AAGTGGATCC 
ATTCTCAATG AACTTATTGG ACTTGTGAGA TCACCCTTAT TGCAGGGGGG AGCTCTTAGT 
GCCATGCTAG ACTTTTTCCA AGCTCTGGTT GTCACTGGAA CAAATAATTT AGGATACATG 
GATTTGTTGC GCATGCTGAC TGGTCCAGTT TACTCTCAGA GCACAGCTCT TACTCATAAG 
CAGTCTTATT ATTCCATTGC CAAATGTGTA GCTGCCCTTA CTCGAGCATG CCCTAAAGAG 
GGACCAGCTG TAGTAGGTCA GTTTATTCAA GATGTCAAGA ACTCAAGGTC TACAGATTCC 
ATTCGTCTCT TAGCTCTACT TTCTCTTGGA GAAGTTGGGC ATCATATTGA CTTAAGTGGA 
CAGTTGGAAC TAAAATCTGT AATACTAGAA GCTTTCTCAT CTCCTAGTGA AGAAGTCAAA 
TCAGCTGCAT CCTATGCATT AGGCAGCATT AGTGTGGGCA ACCTTCCTGA ATATCTGCCG 
TTTGTCCTGC AAGAAATAAC TAGTCAACCC AAAAGGCAGT ATCTTTTACT TCATTCCTTG 
AAGGAAATTA TTAGCTCTGC ATCAGTGGTG GGCCTTAAAC CATATGTTGA AAACATCTGG 
GCCTTATTAC TAAAGCACTG TGAGTGTGCA GAGGAAGGAA CCAGAAATGT TGTTGCTGAA 
TGTCTAGGAA AACTCACTCT AATTGATCCA GAAACTCTCC TTCCACGGCT TAAGGGGTAC 
TTGATATCAG GCTCATCATA TGCCCGAAGC TCAGTGGTTA CGGCTGTGAA ATTTACAATT 
TCTGACCATC CACAACCTAT TGATCCACTG TTAAAGAACT GCATAGGTGA TTTCCTAAAA 
ACTTTGGAAG ACCCAGATTT GAATGTGAGA AGAGTAGCCT TGGTCACATT TAATTCAGCA 
GCACATAACA AGCCATCATT AATAAGGGAT CTATTGGATA CTGTTCTTCC ACATCTTTAC 
AATGAAACAA AAGTTAGAAA GGAGCTTATA AGAGAGGTAG AAATGGGTCC ATTTAAACAT 
ACGGTTGATG ATGGTCTGGA TATTAGAAAG GCAGCATTTG AGTGTATGTA CACACTTCTA 
GACAGTTGTC TTGATAGACT TGATATCTTT GAATTTCTAA ATCATGTTGA AGATGGTTTG 
AAGGACCATT ATGATATTAA GATGCTGACA TTTTTAATGT TGGTGAGACT GTCTACCCTT 
TGTCCAAGTG CAGTACTGCA GAGGTTGGAC CGACTTGTTG AGCCATTACG TGCAACATGT 
ACAACTAAGG TAAAGGCAAA CTCAGTAAAG CAGGAGTTTG AAAAACAAGA TGAATTAAAG 
CGATCTGCCA TGAGAGCAGT AGCAGCACTG CTAACCATTC CAGAAGCAGA GAAGAGTCCA 
CTGATGAGTG AATTCCAGTC ACAGATCAGT TCTAACCCTG AGCTGGCGGC TATCTTTGAA 
AGTATCCAGA AAGATTCATC ATCTACTAAC TTGGAATCAA TGGACACTAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAND1 ( NM_001329676.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001316605.1 Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 4, mRNA.

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CloneID	OHu101809
Clone ID Related Accession (Same CDS sequence)	NM_001329676.2 , NM_001329676.1
Accession Version	NM_001329676.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3594bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-01
Organism	Homo sapiens(human)
Product	cullin-associated NEDD8-dissociated protein 1 isoform 3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC012518.19, AC078983.17 and AA766567.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.93076.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968189 [ECO:0000348] ##Evidence-Data-END##

ATGATTTTGG AATATTTGCA TATACAAAAT GAGATAACTT GGGGATGGAA CCCAAGTCTA 
AACATGAAAT TAATTTATGT TTCATATATA TCTCTTATAC ACATAGCCCG AAGTCTTGGT 
CCTTTAGTGA GTAAAGTGAA AGAATACCAA GTAGAGACAA TTGTAGATAC CCTCTGCACT 
AACATGCTTT CTGATAAAGA ACAACTTCGA GACATTTCAA GTATTGGTCT TAAAACAGTA 
ATTGGAGAAC TTCCTCCAGC TTCCAGTGGC TCTGCATTAG CTGCTAATGT ATGTAAAAAG 
ATTACTGGAC GTCTTACAAG TGCAATAGCA AAACAGGAAG ATGTCTCTGT TCAGCTAGAA 
GCCTTGGATA TTATGGCTGA TATGTTGAGC AGGCAAGGAG GACTTCTTGT TAATTTCCAT 
CCTTCAATTC TGACCTGTCT ACTTCCCCAG TTGACCAGCC CTAGACTTGC AGTGAGGAAA 
AGAACCATTA TCGCTCTTGG CCATCTGGTT ATGAGCTGTG GAAATATAGT TTTTGTAGAT 
CTTATTGAAC ATCTGTTGTC AGAGTTGTCC AAAAATGATT CTATGTCAAC AACAAGAACC 
TACATACAAT GTATTGCTGC TATTAGTAGG CAAGCTGGTC ATAGAATAGG TGAATACCTT 
GAGAAGATAA TTCCTTTGGT GGTAAAATTT TGCAATGTAG ATGATGATGA ATTAAGAGAG 
TACTGTATTC AAGCCTTTGA ATCATTTGTA AGAAGATGTC CTAAGGAAGT ATATCCTCAT 
GTTTCTACCA TTATAAATAT TTGTCTTAAA TATCTTACCT ATGATCCAAA TTATAATTAC 
GATGATGAAG ATGAAGATGA AAATGCAATG GATGCTGATG GTGGTGATGA TGATGATCAA 
GGGAGTGATG ATGAATACAG TGATGATGAT GACATGAGTT GGAAAGTGAG ACGTGCAGCT 
GCGAAGTGCT TGGATGCTGT AGTTAGCACA AGGCATGAAA TGCTTCCAGA ATTCTACAAG 
ACCGTCTCTC CTGCACTAAT ATCCAGATTT AAAGAGCGTG AAGAGAATGT AAAGGCAGAT 
GTTTTTCACG CATACCTTTC TCTTTTGAAG CAAACTCGTC CTGTACAAAG TTGGCTATGT 
GACCCTGATG CAATGGAGCA GGGAGAAACA CCTTTAACAA TGCTTCAGAG TCAGGTTCCC 
AACATTGTTA AAGCTCTTCA CAAACAGATG AAAGAAAAAA GTGTGAAGAC CCGACAGTGT 
TGTTTTAACA TGTTAACTGA GCTGGTAAAT GTATTACCTG GGGCCCTAAC TCAACACATT 
CCTGTACTTG TACCAGGAAT CATTTTCTCA CTGAATGATA AATCAAGCTC ATCGAATTTG 
AAGATCGATG CTTTGTCATG TCTATACGTA ATCCTCTGTA ACCATTCTCC TCAAGTCTTC 
CATCCTCACG TTCAGGCTTT GGTTCCTCCA GTGGTGGCTT GTGTTGGAGA CCCATTTTAC 
AAAATTACAT CTGAAGCACT TCTTGTTACT CAACAGCTTG TCAAAGTAAT TCGTCCTTTA 
GATCAGCCTT CCTCGTTTGA TGCAACTCCT TATATCAAAG ATCTATTTAC CTGTACCATT 
AAGAGATTAA AAGCAGCTGA CATTGATCAG GAAGTCAAGG AAAGGGCTAT TTCCTGTATG 
GGACAAATTA TTTGCAACCT TGGAGACAAT TTGGGTTCTG ACTTGCCTAA TACACTTCAG 
ATTTTCTTGG AGAGACTAAA GAATGAAATT ACCAGGTTAA CTACAGTAAA GGCATTGACA 
CTGATTGCTG GGTCACCTTT GAAGATAGAT TTGAGGCCTG TTCTGGGAGA AGGGGTTCCT 
ATCCTTGCTT CATTTCTTAG AAAAAACCAG AGAGCTTTGA AACTGGGTAC TCTTTCTGCC 
CTTGATATTC TAATAAAAAA CTATAGTGAC AGCTTGACAG CTGCCATGAT TGATGCAGTT 
CTAGATGAGC TCCCACCTCT TATCAGCGAA AGTGATATGC ATGTTTCACA AATGGCCATC 
AGTTTTCTTA CCACTTTGGC AAAAGTATAT CCCTCCTCCC TTTCAAAGAT AAGTGGATCC 
ATTCTCAATG AACTTATTGG ACTTGTGAGA TCACCCTTAT TGCAGGGGGG AGCTCTTAGT 
GCCATGCTAG ACTTTTTCCA AGCTCTGGTT GTCACTGGAA CAAATAATTT AGGATACATG 
GATTTGTTGC GCATGCTGAC TGGTCCAGTT TACTCTCAGA GCACAGCTCT TACTCATAAG 
CAGTCTTATT ATTCCATTGC CAAATGTGTA GCTGCCCTTA CTCGAGCATG CCCTAAAGAG 
GGACCAGCTG TAGTAGGTCA GTTTATTCAA GATGTCAAGA ACTCAAGGTC TACAGATTCC 
ATTCGTCTCT TAGCTCTACT TTCTCTTGGA GAAGTTGGGC ATCATATTGA CTTAAGTGGA 
CAGTTGGAAC TAAAATCTGT AATACTAGAA GCTTTCTCAT CTCCTAGTGA AGAAGTCAAA 
TCAGCTGCAT CCTATGCATT AGGCAGCATT AGTGTGGGCA ACCTTCCTGA ATATCTGCCG 
TTTGTCCTGC AAGAAATAAC TAGTCAACCC AAAAGGCAGT ATCTTTTACT TCATTCCTTG 
AAGGAAATTA TTAGCTCTGC ATCAGTGGTG GGCCTTAAAC CATATGTTGA AAACATCTGG 
GCCTTATTAC TAAAGCACTG TGAGTGTGCA GAGGAAGGAA CCAGAAATGT TGTTGCTGAA 
TGTCTAGGAA AACTCACTCT AATTGATCCA GAAACTCTCC TTCCACGGCT TAAGGGGTAC 
TTGATATCAG GCTCATCATA TGCCCGAAGC TCAGTGGTTA CGGCTGTGAA ATTTACAATT 
TCTGACCATC CACAACCTAT TGATCCACTG TTAAAGAACT GCATAGGTGA TTTCCTAAAA 
ACTTTGGAAG ACCCAGATTT GAATGTGAGA AGAGTAGCCT TGGTCACATT TAATTCAGCA 
GCACATAACA AGCCATCATT AATAAGGGAT CTATTGGATA CTGTTCTTCC ACATCTTTAC 
AATGAAACAA AAGTTAGAAA GGAGCTTATA AGAGAGGTAG AAATGGGTCC ATTTAAACAT 
ACGGTTGATG ATGGTCTGGA TATTAGAAAG GCAGCATTTG AGTGTATGTA CACACTTCTA 
GACAGTTGTC TTGATAGACT TGATATCTTT GAATTTCTAA ATCATGTTGA AGATGGTTTG 
AAGGACCATT ATGATATTAA GATGCTGACA TTTTTAATGT TGGTGAGACT GTCTACCCTT 
TGTCCAAGTG CAGTACTGCA GAGGTTGGAC CGACTTGTTG AGCCATTACG TGCAACATGT 
ACAACTAAGG TAAAGGCAAA CTCAGTAAAG CAGGAGTTTG AAAAACAAGA TGAATTAAAG 
CGATCTGCCA TGAGAGCAGT AGCAGCACTG CTAACCATTC CAGAAGCAGA GAAGAGTCCA 
CTGATGAGTG AATTCCAGTC ACAGATCAGT TCTAACCCTG AGCTGGCGGC TATCTTTGAA 
AGTATCCAGA AAGATTCATC ATCTACTAAC TTGGAATCAA TGGACACTAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316605.1
CDS	647..4240
Translation	MILEYLHIQNEITWGWNPSLNMKLIYVSYISLIHIARSLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS

Target ORF information:

RefSeq Version	NM_001329676.1
Organism	Homo sapiens(human)
Definition	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329676.1

ORF Insert Sequence:

ATGATTTTGG AATATTTGCA TATACAAAAT GAGATAACTT GGGGATGGAA CCCAAGTCTA 
AACATGAAAT TAATTTATGT TTCATATATA TCTCTTATAC ACATAGCCCG AAGTCTTGGT 
CCTTTAGTGA GTAAAGTGAA AGAATACCAA GTAGAGACAA TTGTAGATAC CCTCTGCACT 
AACATGCTTT CTGATAAAGA ACAACTTCGA GACATTTCAA GTATTGGTCT TAAAACAGTA 
ATTGGAGAAC TTCCTCCAGC TTCCAGTGGC TCTGCATTAG CTGCTAATGT ATGTAAAAAG 
ATTACTGGAC GTCTTACAAG TGCAATAGCA AAACAGGAAG ATGTCTCTGT TCAGCTAGAA 
GCCTTGGATA TTATGGCTGA TATGTTGAGC AGGCAAGGAG GACTTCTTGT TAATTTCCAT 
CCTTCAATTC TGACCTGTCT ACTTCCCCAG TTGACCAGCC CTAGACTTGC AGTGAGGAAA 
AGAACCATTA TCGCTCTTGG CCATCTGGTT ATGAGCTGTG GAAATATAGT TTTTGTAGAT 
CTTATTGAAC ATCTGTTGTC AGAGTTGTCC AAAAATGATT CTATGTCAAC AACAAGAACC 
TACATACAAT GTATTGCTGC TATTAGTAGG CAAGCTGGTC ATAGAATAGG TGAATACCTT 
GAGAAGATAA TTCCTTTGGT GGTAAAATTT TGCAATGTAG ATGATGATGA ATTAAGAGAG 
TACTGTATTC AAGCCTTTGA ATCATTTGTA AGAAGATGTC CTAAGGAAGT ATATCCTCAT 
GTTTCTACCA TTATAAATAT TTGTCTTAAA TATCTTACCT ATGATCCAAA TTATAATTAC 
GATGATGAAG ATGAAGATGA AAATGCAATG GATGCTGATG GTGGTGATGA TGATGATCAA 
GGGAGTGATG ATGAATACAG TGATGATGAT GACATGAGTT GGAAAGTGAG ACGTGCAGCT 
GCGAAGTGCT TGGATGCTGT AGTTAGCACA AGGCATGAAA TGCTTCCAGA ATTCTACAAG 
ACCGTCTCTC CTGCACTAAT ATCCAGATTT AAAGAGCGTG AAGAGAATGT AAAGGCAGAT 
GTTTTTCACG CATACCTTTC TCTTTTGAAG CAAACTCGTC CTGTACAAAG TTGGCTATGT 
GACCCTGATG CAATGGAGCA GGGAGAAACA CCTTTAACAA TGCTTCAGAG TCAGGTTCCC 
AACATTGTTA AAGCTCTTCA CAAACAGATG AAAGAAAAAA GTGTGAAGAC CCGACAGTGT 
TGTTTTAACA TGTTAACTGA GCTGGTAAAT GTATTACCTG GGGCCCTAAC TCAACACATT 
CCTGTACTTG TACCAGGAAT CATTTTCTCA CTGAATGATA AATCAAGCTC ATCGAATTTG 
AAGATCGATG CTTTGTCATG TCTATACGTA ATCCTCTGTA ACCATTCTCC TCAAGTCTTC 
CATCCTCACG TTCAGGCTTT GGTTCCTCCA GTGGTGGCTT GTGTTGGAGA CCCATTTTAC 
AAAATTACAT CTGAAGCACT TCTTGTTACT CAACAGCTTG TCAAAGTAAT TCGTCCTTTA 
GATCAGCCTT CCTCGTTTGA TGCAACTCCT TATATCAAAG ATCTATTTAC CTGTACCATT 
AAGAGATTAA AAGCAGCTGA CATTGATCAG GAAGTCAAGG AAAGGGCTAT TTCCTGTATG 
GGACAAATTA TTTGCAACCT TGGAGACAAT TTGGGTTCTG ACTTGCCTAA TACACTTCAG 
ATTTTCTTGG AGAGACTAAA GAATGAAATT ACCAGGTTAA CTACAGTAAA GGCATTGACA 
CTGATTGCTG GGTCACCTTT GAAGATAGAT TTGAGGCCTG TTCTGGGAGA AGGGGTTCCT 
ATCCTTGCTT CATTTCTTAG AAAAAACCAG AGAGCTTTGA AACTGGGTAC TCTTTCTGCC 
CTTGATATTC TAATAAAAAA CTATAGTGAC AGCTTGACAG CTGCCATGAT TGATGCAGTT 
CTAGATGAGC TCCCACCTCT TATCAGCGAA AGTGATATGC ATGTTTCACA AATGGCCATC 
AGTTTTCTTA CCACTTTGGC AAAAGTATAT CCCTCCTCCC TTTCAAAGAT AAGTGGATCC 
ATTCTCAATG AACTTATTGG ACTTGTGAGA TCACCCTTAT TGCAGGGGGG AGCTCTTAGT 
GCCATGCTAG ACTTTTTCCA AGCTCTGGTT GTCACTGGAA CAAATAATTT AGGATACATG 
GATTTGTTGC GCATGCTGAC TGGTCCAGTT TACTCTCAGA GCACAGCTCT TACTCATAAG 
CAGTCTTATT ATTCCATTGC CAAATGTGTA GCTGCCCTTA CTCGAGCATG CCCTAAAGAG 
GGACCAGCTG TAGTAGGTCA GTTTATTCAA GATGTCAAGA ACTCAAGGTC TACAGATTCC 
ATTCGTCTCT TAGCTCTACT TTCTCTTGGA GAAGTTGGGC ATCATATTGA CTTAAGTGGA 
CAGTTGGAAC TAAAATCTGT AATACTAGAA GCTTTCTCAT CTCCTAGTGA AGAAGTCAAA 
TCAGCTGCAT CCTATGCATT AGGCAGCATT AGTGTGGGCA ACCTTCCTGA ATATCTGCCG 
TTTGTCCTGC AAGAAATAAC TAGTCAACCC AAAAGGCAGT ATCTTTTACT TCATTCCTTG 
AAGGAAATTA TTAGCTCTGC ATCAGTGGTG GGCCTTAAAC CATATGTTGA AAACATCTGG 
GCCTTATTAC TAAAGCACTG TGAGTGTGCA GAGGAAGGAA CCAGAAATGT TGTTGCTGAA 
TGTCTAGGAA AACTCACTCT AATTGATCCA GAAACTCTCC TTCCACGGCT TAAGGGGTAC 
TTGATATCAG GCTCATCATA TGCCCGAAGC TCAGTGGTTA CGGCTGTGAA ATTTACAATT 
TCTGACCATC CACAACCTAT TGATCCACTG TTAAAGAACT GCATAGGTGA TTTCCTAAAA 
ACTTTGGAAG ACCCAGATTT GAATGTGAGA AGAGTAGCCT TGGTCACATT TAATTCAGCA 
GCACATAACA AGCCATCATT AATAAGGGAT CTATTGGATA CTGTTCTTCC ACATCTTTAC 
AATGAAACAA AAGTTAGAAA GGAGCTTATA AGAGAGGTAG AAATGGGTCC ATTTAAACAT 
ACGGTTGATG ATGGTCTGGA TATTAGAAAG GCAGCATTTG AGTGTATGTA CACACTTCTA 
GACAGTTGTC TTGATAGACT TGATATCTTT GAATTTCTAA ATCATGTTGA AGATGGTTTG 
AAGGACCATT ATGATATTAA GATGCTGACA TTTTTAATGT TGGTGAGACT GTCTACCCTT 
TGTCCAAGTG CAGTACTGCA GAGGTTGGAC CGACTTGTTG AGCCATTACG TGCAACATGT 
ACAACTAAGG TAAAGGCAAA CTCAGTAAAG CAGGAGTTTG AAAAACAAGA TGAATTAAAG 
CGATCTGCCA TGAGAGCAGT AGCAGCACTG CTAACCATTC CAGAAGCAGA GAAGAGTCCA 
CTGATGAGTG AATTCCAGTC ACAGATCAGT TCTAACCCTG AGCTGGCGGC TATCTTTGAA 
AGTATCCAGA AAGATTCATC ATCTACTAAC TTGGAATCAA TGGACACTAG TTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAND1 ( NM_018448.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060918.2 Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31371
Clone ID Related Accession (Same CDS sequence)	NM_018448.5 , NM_018448.4
Accession Version	NM_018448.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-26
Organism	Homo sapiens(human)
Product	cullin-associated NEDD8-dissociated protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB064887.1, AL136810.1, AB020636.2, BQ024785.1 and AC078983.17. On Nov 23, 2018 this sequence version replaced NM_018448.4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803612.200182.1, SRR1803616.9626.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000545606.6/ ENSP00000442318.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGCGAGCG CCTCGTACCA CATTTCCAAT TTGCTGGAAA AAATGACATC CAGCGACAAG 
GACTTTAGGT TTATGGCTAC AAATGATTTG ATGACGGAAC TGCAGAAAGA TTCCATCAAG 
TTGGATGATG ATAGTGAAAG GAAAGTAGTG AAAATGATTT TGAAGTTATT GGAAGATAAA 
AATGGAGAGG TACAGAATTT AGCTGTCAAA TGTCTTGGTC CTTTAGTGAG TAAAGTGAAA 
GAATACCAAG TAGAGACAAT TGTAGATACC CTCTGCACTA ACATGCTTTC TGATAAAGAA 
CAACTTCGAG ACATTTCAAG TATTGGTCTT AAAACAGTAA TTGGAGAACT TCCTCCAGCT 
TCCAGTGGCT CTGCATTAGC TGCTAATGTA TGTAAAAAGA TTACTGGACG TCTTACAAGT 
GCAATAGCAA AACAGGAAGA TGTCTCTGTT CAGCTAGAAG CCTTGGATAT TATGGCTGAT 
ATGTTGAGCA GGCAAGGAGG ACTTCTTGTT AATTTCCATC CTTCAATTCT GACCTGTCTA 
CTTCCCCAGT TGACCAGCCC TAGACTTGCA GTGAGGAAAA GAACCATTAT CGCTCTTGGC 
CATCTGGTTA TGAGCTGTGG AAATATAGTT TTTGTAGATC TTATTGAACA TCTGTTGTCA 
GAGTTGTCCA AAAATGATTC TATGTCAACA ACAAGAACCT ACATACAATG TATTGCTGCT 
ATTAGTAGGC AAGCTGGTCA TAGAATAGGT GAATACCTTG AGAAGATAAT TCCTTTGGTG 
GTAAAATTTT GCAATGTAGA TGATGATGAA TTAAGAGAGT ACTGTATTCA AGCCTTTGAA 
TCATTTGTAA GAAGATGTCC TAAGGAAGTA TATCCTCATG TTTCTACCAT TATAAATATT 
TGTCTTAAAT ATCTTACCTA TGATCCAAAT TATAATTACG ATGATGAAGA TGAAGATGAA 
AATGCAATGG ATGCTGATGG TGGTGATGAT GATGATCAAG GGAGTGATGA TGAATACAGT 
GATGATGATG ACATGAGTTG GAAAGTGAGA CGTGCAGCTG CGAAGTGCTT GGATGCTGTA 
GTTAGCACAA GGCATGAAAT GCTTCCAGAA TTCTACAAGA CCGTCTCTCC TGCACTAATA 
TCCAGATTTA AAGAGCGTGA AGAGAATGTA AAGGCAGATG TTTTTCACGC ATACCTTTCT 
CTTTTGAAGC AAACTCGTCC TGTACAAAGT TGGCTATGTG ACCCTGATGC AATGGAGCAG 
GGAGAAACAC CTTTAACAAT GCTTCAGAGT CAGGTTCCCA ACATTGTTAA AGCTCTTCAC 
AAACAGATGA AAGAAAAAAG TGTGAAGACC CGACAGTGTT GTTTTAACAT GTTAACTGAG 
CTGGTAAATG TATTACCTGG GGCCCTAACT CAACACATTC CTGTACTTGT ACCAGGAATC 
ATTTTCTCAC TGAATGATAA ATCAAGCTCA TCGAATTTGA AGATCGATGC TTTGTCATGT 
CTATACGTAA TCCTCTGTAA CCATTCTCCT CAAGTCTTCC ATCCTCACGT TCAGGCTTTG 
GTTCCTCCAG TGGTGGCTTG TGTTGGAGAC CCATTTTACA AAATTACATC TGAAGCACTT 
CTTGTTACTC AACAGCTTGT CAAAGTAATT CGTCCTTTAG ATCAGCCTTC CTCGTTTGAT 
GCAACTCCTT ATATCAAAGA TCTATTTACC TGTACCATTA AGAGATTAAA AGCAGCTGAC 
ATTGATCAGG AAGTCAAGGA AAGGGCTATT TCCTGTATGG GACAAATTAT TTGCAACCTT 
GGAGACAATT TGGGTTCTGA CTTGCCTAAT ACACTTCAGA TTTTCTTGGA GAGACTAAAG 
AATGAAATTA CCAGGTTAAC TACAGTAAAG GCATTGACAC TGATTGCTGG GTCACCTTTG 
AAGATAGATT TGAGGCCTGT TCTGGGAGAA GGGGTTCCTA TCCTTGCTTC ATTTCTTAGA 
AAAAACCAGA GAGCTTTGAA ACTGGGTACT CTTTCTGCCC TTGATATTCT AATAAAAAAC 
TATAGTGACA GCTTGACAGC TGCCATGATT GATGCAGTTC TAGATGAGCT CCCACCTCTT 
ATCAGCGAAA GTGATATGCA TGTTTCACAA ATGGCCATCA GTTTTCTTAC CACTTTGGCA 
AAAGTATATC CCTCCTCCCT TTCAAAGATA AGTGGATCCA TTCTCAATGA ACTTATTGGA 
CTTGTGAGAT CACCCTTATT GCAGGGGGGA GCTCTTAGTG CCATGCTAGA CTTTTTCCAA 
GCTCTGGTTG TCACTGGAAC AAATAATTTA GGATACATGG ATTTGTTGCG CATGCTGACT 
GGTCCAGTTT ACTCTCAGAG CACAGCTCTT ACTCATAAGC AGTCTTATTA TTCCATTGCC 
AAATGTGTAG CTGCCCTTAC TCGAGCATGC CCTAAAGAGG GACCAGCTGT AGTAGGTCAG 
TTTATTCAAG ATGTCAAGAA CTCAAGGTCT ACAGATTCCA TTCGTCTCTT AGCTCTACTT 
TCTCTTGGAG AAGTTGGGCA TCATATTGAC TTAAGTGGAC AGTTGGAACT AAAATCTGTA 
ATACTAGAAG CTTTCTCATC TCCTAGTGAA GAAGTCAAAT CAGCTGCATC CTATGCATTA 
GGCAGCATTA GTGTGGGCAA CCTTCCTGAA TATCTGCCGT TTGTCCTGCA AGAAATAACT 
AGTCAACCCA AAAGGCAGTA TCTTTTACTT CATTCCTTGA AGGAAATTAT TAGCTCTGCA 
TCAGTGGTGG GCCTTAAACC ATATGTTGAA AACATCTGGG CCTTATTACT AAAGCACTGT 
GAGTGTGCAG AGGAAGGAAC CAGAAATGTT GTTGCTGAAT GTCTAGGAAA ACTCACTCTA 
ATTGATCCAG AAACTCTCCT TCCACGGCTT AAGGGGTACT TGATATCAGG CTCATCATAT 
GCCCGAAGCT CAGTGGTTAC GGCTGTGAAA TTTACAATTT CTGACCATCC ACAACCTATT 
GATCCACTGT TAAAGAACTG CATAGGTGAT TTCCTAAAAA CTTTGGAAGA CCCAGATTTG 
AATGTGAGAA GAGTAGCCTT GGTCACATTT AATTCAGCAG CACATAACAA GCCATCATTA 
ATAAGGGATC TATTGGATAC TGTTCTTCCA CATCTTTACA ATGAAACAAA AGTTAGAAAG 
GAGCTTATAA GAGAGGTAGA AATGGGTCCA TTTAAACATA CGGTTGATGA TGGTCTGGAT 
ATTAGAAAGG CAGCATTTGA GTGTATGTAC ACACTTCTAG ACAGTTGTCT TGATAGACTT 
GATATCTTTG AATTTCTAAA TCATGTTGAA GATGGTTTGA AGGACCATTA TGATATTAAG 
ATGCTGACAT TTTTAATGTT GGTGAGACTG TCTACCCTTT GTCCAAGTGC AGTACTGCAG 
AGGTTGGACC GACTTGTTGA GCCATTACGT GCAACATGTA CAACTAAGGT AAAGGCAAAC 
TCAGTAAAGC AGGAGTTTGA AAAACAAGAT GAATTAAAGC GATCTGCCAT GAGAGCAGTA 
GCAGCACTGC TAACCATTCC AGAAGCAGAG AAGAGTCCAC TGATGAGTGA ATTCCAGTCA 
CAGATCAGTT CTAACCCTGA GCTGGCGGCT ATCTTTGAAA GTATCCAGAA AGATTCATCA 
TCTACTAACT TGGAATCAAT GGACACTAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060918.2
CDS	361..4053
Translation	MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS

Target ORF information:

RefSeq Version	NM_018448.5
Organism	Homo sapiens(human)
Definition	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018448.5

ORF Insert Sequence:

ATGGCGAGCG CCTCGTACCA CATTTCCAAT TTGCTGGAAA AAATGACATC CAGCGACAAG 
GACTTTAGGT TTATGGCTAC AAATGATTTG ATGACGGAAC TGCAGAAAGA TTCCATCAAG 
TTGGATGATG ATAGTGAAAG GAAAGTAGTG AAAATGATTT TGAAGTTATT GGAAGATAAA 
AATGGAGAGG TACAGAATTT AGCTGTCAAA TGTCTTGGTC CTTTAGTGAG TAAAGTGAAA 
GAATACCAAG TAGAGACAAT TGTAGATACC CTCTGCACTA ACATGCTTTC TGATAAAGAA 
CAACTTCGAG ACATTTCAAG TATTGGTCTT AAAACAGTAA TTGGAGAACT TCCTCCAGCT 
TCCAGTGGCT CTGCATTAGC TGCTAATGTA TGTAAAAAGA TTACTGGACG TCTTACAAGT 
GCAATAGCAA AACAGGAAGA TGTCTCTGTT CAGCTAGAAG CCTTGGATAT TATGGCTGAT 
ATGTTGAGCA GGCAAGGAGG ACTTCTTGTT AATTTCCATC CTTCAATTCT GACCTGTCTA 
CTTCCCCAGT TGACCAGCCC TAGACTTGCA GTGAGGAAAA GAACCATTAT CGCTCTTGGC 
CATCTGGTTA TGAGCTGTGG AAATATAGTT TTTGTAGATC TTATTGAACA TCTGTTGTCA 
GAGTTGTCCA AAAATGATTC TATGTCAACA ACAAGAACCT ACATACAATG TATTGCTGCT 
ATTAGTAGGC AAGCTGGTCA TAGAATAGGT GAATACCTTG AGAAGATAAT TCCTTTGGTG 
GTAAAATTTT GCAATGTAGA TGATGATGAA TTAAGAGAGT ACTGTATTCA AGCCTTTGAA 
TCATTTGTAA GAAGATGTCC TAAGGAAGTA TATCCTCATG TTTCTACCAT TATAAATATT 
TGTCTTAAAT ATCTTACCTA TGATCCAAAT TATAATTACG ATGATGAAGA TGAAGATGAA 
AATGCAATGG ATGCTGATGG TGGTGATGAT GATGATCAAG GGAGTGATGA TGAATACAGT 
GATGATGATG ACATGAGTTG GAAAGTGAGA CGTGCAGCTG CGAAGTGCTT GGATGCTGTA 
GTTAGCACAA GGCATGAAAT GCTTCCAGAA TTCTACAAGA CCGTCTCTCC TGCACTAATA 
TCCAGATTTA AAGAGCGTGA AGAGAATGTA AAGGCAGATG TTTTTCACGC ATACCTTTCT 
CTTTTGAAGC AAACTCGTCC TGTACAAAGT TGGCTATGTG ACCCTGATGC AATGGAGCAG 
GGAGAAACAC CTTTAACAAT GCTTCAGAGT CAGGTTCCCA ACATTGTTAA AGCTCTTCAC 
AAACAGATGA AAGAAAAAAG TGTGAAGACC CGACAGTGTT GTTTTAACAT GTTAACTGAG 
CTGGTAAATG TATTACCTGG GGCCCTAACT CAACACATTC CTGTACTTGT ACCAGGAATC 
ATTTTCTCAC TGAATGATAA ATCAAGCTCA TCGAATTTGA AGATCGATGC TTTGTCATGT 
CTATACGTAA TCCTCTGTAA CCATTCTCCT CAAGTCTTCC ATCCTCACGT TCAGGCTTTG 
GTTCCTCCAG TGGTGGCTTG TGTTGGAGAC CCATTTTACA AAATTACATC TGAAGCACTT 
CTTGTTACTC AACAGCTTGT CAAAGTAATT CGTCCTTTAG ATCAGCCTTC CTCGTTTGAT 
GCAACTCCTT ATATCAAAGA TCTATTTACC TGTACCATTA AGAGATTAAA AGCAGCTGAC 
ATTGATCAGG AAGTCAAGGA AAGGGCTATT TCCTGTATGG GACAAATTAT TTGCAACCTT 
GGAGACAATT TGGGTTCTGA CTTGCCTAAT ACACTTCAGA TTTTCTTGGA GAGACTAAAG 
AATGAAATTA CCAGGTTAAC TACAGTAAAG GCATTGACAC TGATTGCTGG GTCACCTTTG 
AAGATAGATT TGAGGCCTGT TCTGGGAGAA GGGGTTCCTA TCCTTGCTTC ATTTCTTAGA 
AAAAACCAGA GAGCTTTGAA ACTGGGTACT CTTTCTGCCC TTGATATTCT AATAAAAAAC 
TATAGTGACA GCTTGACAGC TGCCATGATT GATGCAGTTC TAGATGAGCT CCCACCTCTT 
ATCAGCGAAA GTGATATGCA TGTTTCACAA ATGGCCATCA GTTTTCTTAC CACTTTGGCA 
AAAGTATATC CCTCCTCCCT TTCAAAGATA AGTGGATCCA TTCTCAATGA ACTTATTGGA 
CTTGTGAGAT CACCCTTATT GCAGGGGGGA GCTCTTAGTG CCATGCTAGA CTTTTTCCAA 
GCTCTGGTTG TCACTGGAAC AAATAATTTA GGATACATGG ATTTGTTGCG CATGCTGACT 
GGTCCAGTTT ACTCTCAGAG CACAGCTCTT ACTCATAAGC AGTCTTATTA TTCCATTGCC 
AAATGTGTAG CTGCCCTTAC TCGAGCATGC CCTAAAGAGG GACCAGCTGT AGTAGGTCAG 
TTTATTCAAG ATGTCAAGAA CTCAAGGTCT ACAGATTCCA TTCGTCTCTT AGCTCTACTT 
TCTCTTGGAG AAGTTGGGCA TCATATTGAC TTAAGTGGAC AGTTGGAACT AAAATCTGTA 
ATACTAGAAG CTTTCTCATC TCCTAGTGAA GAAGTCAAAT CAGCTGCATC CTATGCATTA 
GGCAGCATTA GTGTGGGCAA CCTTCCTGAA TATCTGCCGT TTGTCCTGCA AGAAATAACT 
AGTCAACCCA AAAGGCAGTA TCTTTTACTT CATTCCTTGA AGGAAATTAT TAGCTCTGCA 
TCAGTGGTGG GCCTTAAACC ATATGTTGAA AACATCTGGG CCTTATTACT AAAGCACTGT 
GAGTGTGCAG AGGAAGGAAC CAGAAATGTT GTTGCTGAAT GTCTAGGAAA ACTCACTCTA 
ATTGATCCAG AAACTCTCCT TCCACGGCTT AAGGGGTACT TGATATCAGG CTCATCATAT 
GCCCGAAGCT CAGTGGTTAC GGCTGTGAAA TTTACAATTT CTGACCATCC ACAACCTATT 
GATCCACTGT TAAAGAACTG CATAGGTGAT TTCCTAAAAA CTTTGGAAGA CCCAGATTTG 
AATGTGAGAA GAGTAGCCTT GGTCACATTT AATTCAGCAG CACATAACAA GCCATCATTA 
ATAAGGGATC TATTGGATAC TGTTCTTCCA CATCTTTACA ATGAAACAAA AGTTAGAAAG 
GAGCTTATAA GAGAGGTAGA AATGGGTCCA TTTAAACATA CGGTTGATGA TGGTCTGGAT 
ATTAGAAAGG CAGCATTTGA GTGTATGTAC ACACTTCTAG ACAGTTGTCT TGATAGACTT 
GATATCTTTG AATTTCTAAA TCATGTTGAA GATGGTTTGA AGGACCATTA TGATATTAAG 
ATGCTGACAT TTTTAATGTT GGTGAGACTG TCTACCCTTT GTCCAAGTGC AGTACTGCAG 
AGGTTGGACC GACTTGTTGA GCCATTACGT GCAACATGTA CAACTAAGGT AAAGGCAAAC 
TCAGTAAAGC AGGAGTTTGA AAAACAAGAT GAATTAAAGC GATCTGCCAT GAGAGCAGTA 
GCAGCACTGC TAACCATTCC AGAAGCAGAG AAGAGTCCAC TGATGAGTGA ATTCCAGTCA 
CAGATCAGTT CTAACCCTGA GCTGGCGGCT ATCTTTGAAA GTATCCAGAA AGATTCATCA 
TCTACTAACT TGGAATCAAT GGACACTAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CAND1 ( NM_018448.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060918.2 Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu31371
Clone ID Related Accession (Same CDS sequence)	NM_018448.5 , NM_018448.4
Accession Version	NM_018448.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-24
Organism	Homo sapiens(human)
Product	cullin-associated NEDD8-dissociated protein 1 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB064887.1, AL136810.1, AB020636.2 and BQ024785.1. This sequence is a reference standard in the RefSeqGene project. On Jan 23, 2015 this sequence version replaced NM_018448.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.128103.1, SRR1803613.176951.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGCGAGCG CCTCGTACCA CATTTCCAAT TTGCTGGAAA AAATGACATC CAGCGACAAG 
GACTTTAGGT TTATGGCTAC AAATGATTTG ATGACGGAAC TGCAGAAAGA TTCCATCAAG 
TTGGATGATG ATAGTGAAAG GAAAGTAGTG AAAATGATTT TGAAGTTATT GGAAGATAAA 
AATGGAGAGG TACAGAATTT AGCTGTCAAA TGTCTTGGTC CTTTAGTGAG TAAAGTGAAA 
GAATACCAAG TAGAGACAAT TGTAGATACC CTCTGCACTA ACATGCTTTC TGATAAAGAA 
CAACTTCGAG ACATTTCAAG TATTGGTCTT AAAACAGTAA TTGGAGAACT TCCTCCAGCT 
TCCAGTGGCT CTGCATTAGC TGCTAATGTA TGTAAAAAGA TTACTGGACG TCTTACAAGT 
GCAATAGCAA AACAGGAAGA TGTCTCTGTT CAGCTAGAAG CCTTGGATAT TATGGCTGAT 
ATGTTGAGCA GGCAAGGAGG ACTTCTTGTT AATTTCCATC CTTCAATTCT GACCTGTCTA 
CTTCCCCAGT TGACCAGCCC TAGACTTGCA GTGAGGAAAA GAACCATTAT CGCTCTTGGC 
CATCTGGTTA TGAGCTGTGG AAATATAGTT TTTGTAGATC TTATTGAACA TCTGTTGTCA 
GAGTTGTCCA AAAATGATTC TATGTCAACA ACAAGAACCT ACATACAATG TATTGCTGCT 
ATTAGTAGGC AAGCTGGTCA TAGAATAGGT GAATACCTTG AGAAGATAAT TCCTTTGGTG 
GTAAAATTTT GCAATGTAGA TGATGATGAA TTAAGAGAGT ACTGTATTCA AGCCTTTGAA 
TCATTTGTAA GAAGATGTCC TAAGGAAGTA TATCCTCATG TTTCTACCAT TATAAATATT 
TGTCTTAAAT ATCTTACCTA TGATCCAAAT TATAATTACG ATGATGAAGA TGAAGATGAA 
AATGCAATGG ATGCTGATGG TGGTGATGAT GATGATCAAG GGAGTGATGA TGAATACAGT 
GATGATGATG ACATGAGTTG GAAAGTGAGA CGTGCAGCTG CGAAGTGCTT GGATGCTGTA 
GTTAGCACAA GGCATGAAAT GCTTCCAGAA TTCTACAAGA CCGTCTCTCC TGCACTAATA 
TCCAGATTTA AAGAGCGTGA AGAGAATGTA AAGGCAGATG TTTTTCACGC ATACCTTTCT 
CTTTTGAAGC AAACTCGTCC TGTACAAAGT TGGCTATGTG ACCCTGATGC AATGGAGCAG 
GGAGAAACAC CTTTAACAAT GCTTCAGAGT CAGGTTCCCA ACATTGTTAA AGCTCTTCAC 
AAACAGATGA AAGAAAAAAG TGTGAAGACC CGACAGTGTT GTTTTAACAT GTTAACTGAG 
CTGGTAAATG TATTACCTGG GGCCCTAACT CAACACATTC CTGTACTTGT ACCAGGAATC 
ATTTTCTCAC TGAATGATAA ATCAAGCTCA TCGAATTTGA AGATCGATGC TTTGTCATGT 
CTATACGTAA TCCTCTGTAA CCATTCTCCT CAAGTCTTCC ATCCTCACGT TCAGGCTTTG 
GTTCCTCCAG TGGTGGCTTG TGTTGGAGAC CCATTTTACA AAATTACATC TGAAGCACTT 
CTTGTTACTC AACAGCTTGT CAAAGTAATT CGTCCTTTAG ATCAGCCTTC CTCGTTTGAT 
GCAACTCCTT ATATCAAAGA TCTATTTACC TGTACCATTA AGAGATTAAA AGCAGCTGAC 
ATTGATCAGG AAGTCAAGGA AAGGGCTATT TCCTGTATGG GACAAATTAT TTGCAACCTT 
GGAGACAATT TGGGTTCTGA CTTGCCTAAT ACACTTCAGA TTTTCTTGGA GAGACTAAAG 
AATGAAATTA CCAGGTTAAC TACAGTAAAG GCATTGACAC TGATTGCTGG GTCACCTTTG 
AAGATAGATT TGAGGCCTGT TCTGGGAGAA GGGGTTCCTA TCCTTGCTTC ATTTCTTAGA 
AAAAACCAGA GAGCTTTGAA ACTGGGTACT CTTTCTGCCC TTGATATTCT AATAAAAAAC 
TATAGTGACA GCTTGACAGC TGCCATGATT GATGCAGTTC TAGATGAGCT CCCACCTCTT 
ATCAGCGAAA GTGATATGCA TGTTTCACAA ATGGCCATCA GTTTTCTTAC CACTTTGGCA 
AAAGTATATC CCTCCTCCCT TTCAAAGATA AGTGGATCCA TTCTCAATGA ACTTATTGGA 
CTTGTGAGAT CACCCTTATT GCAGGGGGGA GCTCTTAGTG CCATGCTAGA CTTTTTCCAA 
GCTCTGGTTG TCACTGGAAC AAATAATTTA GGATACATGG ATTTGTTGCG CATGCTGACT 
GGTCCAGTTT ACTCTCAGAG CACAGCTCTT ACTCATAAGC AGTCTTATTA TTCCATTGCC 
AAATGTGTAG CTGCCCTTAC TCGAGCATGC CCTAAAGAGG GACCAGCTGT AGTAGGTCAG 
TTTATTCAAG ATGTCAAGAA CTCAAGGTCT ACAGATTCCA TTCGTCTCTT AGCTCTACTT 
TCTCTTGGAG AAGTTGGGCA TCATATTGAC TTAAGTGGAC AGTTGGAACT AAAATCTGTA 
ATACTAGAAG CTTTCTCATC TCCTAGTGAA GAAGTCAAAT CAGCTGCATC CTATGCATTA 
GGCAGCATTA GTGTGGGCAA CCTTCCTGAA TATCTGCCGT TTGTCCTGCA AGAAATAACT 
AGTCAACCCA AAAGGCAGTA TCTTTTACTT CATTCCTTGA AGGAAATTAT TAGCTCTGCA 
TCAGTGGTGG GCCTTAAACC ATATGTTGAA AACATCTGGG CCTTATTACT AAAGCACTGT 
GAGTGTGCAG AGGAAGGAAC CAGAAATGTT GTTGCTGAAT GTCTAGGAAA ACTCACTCTA 
ATTGATCCAG AAACTCTCCT TCCACGGCTT AAGGGGTACT TGATATCAGG CTCATCATAT 
GCCCGAAGCT CAGTGGTTAC GGCTGTGAAA TTTACAATTT CTGACCATCC ACAACCTATT 
GATCCACTGT TAAAGAACTG CATAGGTGAT TTCCTAAAAA CTTTGGAAGA CCCAGATTTG 
AATGTGAGAA GAGTAGCCTT GGTCACATTT AATTCAGCAG CACATAACAA GCCATCATTA 
ATAAGGGATC TATTGGATAC TGTTCTTCCA CATCTTTACA ATGAAACAAA AGTTAGAAAG 
GAGCTTATAA GAGAGGTAGA AATGGGTCCA TTTAAACATA CGGTTGATGA TGGTCTGGAT 
ATTAGAAAGG CAGCATTTGA GTGTATGTAC ACACTTCTAG ACAGTTGTCT TGATAGACTT 
GATATCTTTG AATTTCTAAA TCATGTTGAA GATGGTTTGA AGGACCATTA TGATATTAAG 
ATGCTGACAT TTTTAATGTT GGTGAGACTG TCTACCCTTT GTCCAAGTGC AGTACTGCAG 
AGGTTGGACC GACTTGTTGA GCCATTACGT GCAACATGTA CAACTAAGGT AAAGGCAAAC 
TCAGTAAAGC AGGAGTTTGA AAAACAAGAT GAATTAAAGC GATCTGCCAT GAGAGCAGTA 
GCAGCACTGC TAACCATTCC AGAAGCAGAG AAGAGTCCAC TGATGAGTGA ATTCCAGTCA 
CAGATCAGTT CTAACCCTGA GCTGGCGGCT ATCTTTGAAA GTATCCAGAA AGATTCATCA 
TCTACTAACT TGGAATCAAT GGACACTAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060918.2
CDS	438..4130
Translation	MASASYHISNLLEKMTSSDKDFRFMATNDLMTELQKDSIKLDDDSERKVVKMILKLLEDKNGEVQNLAVKCLGPLVSKVKEYQVETIVDTLCTNMLSDKEQLRDISSIGLKTVIGELPPASSGSALAANVCKKITGRLTSAIAKQEDVSVQLEALDIMADMLSRQGGLLVNFHPSILTCLLPQLTSPRLAVRKRTIIALGHLVMSCGNIVFVDLIEHLLSELSKNDSMSTTRTYIQCIAAISRQAGHRIGEYLEKIIPLVVKFCNVDDDELREYCIQAFESFVRRCPKEVYPHVSTIINICLKYLTYDPNYNYDDEDEDENAMDADGGDDDDQGSDDEYSDDDDMSWKVRRAAAKCLDAVVSTRHEMLPEFYKTVSPALISRFKEREENVKADVFHAYLSLLKQTRPVQSWLCDPDAMEQGETPLTMLQSQVPNIVKALHKQMKEKSVKTRQCCFNMLTELVNVLPGALTQHIPVLVPGIIFSLNDKSSSSNLKIDALSCLYVILCNHSPQVFHPHVQALVPPVVACVGDPFYKITSEALLVTQQLVKVIRPLDQPSSFDATPYIKDLFTCTIKRLKAADIDQEVKERAISCMGQIICNLGDNLGSDLPNTLQIFLERLKNEITRLTTVKALTLIAGSPLKIDLRPVLGEGVPILASFLRKNQRALKLGTLSALDILIKNYSDSLTAAMIDAVLDELPPLISESDMHVSQMAISFLTTLAKVYPSSLSKISGSILNELIGLVRSPLLQGGALSAMLDFFQALVVTGTNNLGYMDLLRMLTGPVYSQSTALTHKQSYYSIAKCVAALTRACPKEGPAVVGQFIQDVKNSRSTDSIRLLALLSLGEVGHHIDLSGQLELKSVILEAFSSPSEEVKSAASYALGSISVGNLPEYLPFVLQEITSQPKRQYLLLHSLKEIISSASVVGLKPYVENIWALLLKHCECAEEGTRNVVAECLGKLTLIDPETLLPRLKGYLISGSSYARSSVVTAVKFTISDHPQPIDPLLKNCIGDFLKTLEDPDLNVRRVALVTFNSAAHNKPSLIRDLLDTVLPHLYNETKVRKELIREVEMGPFKHTVDDGLDIRKAAFECMYTLLDSCLDRLDIFEFLNHVEDGLKDHYDIKMLTFLMLVRLSTLCPSAVLQRLDRLVEPLRATCTTKVKANSVKQEFEKQDELKRSAMRAVAALLTIPEAEKSPLMSEFQSQISSNPELAAIFESIQKDSSSTNLESMDTS

Target ORF information:

RefSeq Version	NM_018448.4
Organism	Homo sapiens(human)
Definition	Homo sapiens cullin associated and neddylation dissociated 1 (CAND1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018448.4

ORF Insert Sequence:

ATGGCGAGCG CCTCGTACCA CATTTCCAAT TTGCTGGAAA AAATGACATC CAGCGACAAG 
GACTTTAGGT TTATGGCTAC AAATGATTTG ATGACGGAAC TGCAGAAAGA TTCCATCAAG 
TTGGATGATG ATAGTGAAAG GAAAGTAGTG AAAATGATTT TGAAGTTATT GGAAGATAAA 
AATGGAGAGG TACAGAATTT AGCTGTCAAA TGTCTTGGTC CTTTAGTGAG TAAAGTGAAA 
GAATACCAAG TAGAGACAAT TGTAGATACC CTCTGCACTA ACATGCTTTC TGATAAAGAA 
CAACTTCGAG ACATTTCAAG TATTGGTCTT AAAACAGTAA TTGGAGAACT TCCTCCAGCT 
TCCAGTGGCT CTGCATTAGC TGCTAATGTA TGTAAAAAGA TTACTGGACG TCTTACAAGT 
GCAATAGCAA AACAGGAAGA TGTCTCTGTT CAGCTAGAAG CCTTGGATAT TATGGCTGAT 
ATGTTGAGCA GGCAAGGAGG ACTTCTTGTT AATTTCCATC CTTCAATTCT GACCTGTCTA 
CTTCCCCAGT TGACCAGCCC TAGACTTGCA GTGAGGAAAA GAACCATTAT CGCTCTTGGC 
CATCTGGTTA TGAGCTGTGG AAATATAGTT TTTGTAGATC TTATTGAACA TCTGTTGTCA 
GAGTTGTCCA AAAATGATTC TATGTCAACA ACAAGAACCT ACATACAATG TATTGCTGCT 
ATTAGTAGGC AAGCTGGTCA TAGAATAGGT GAATACCTTG AGAAGATAAT TCCTTTGGTG 
GTAAAATTTT GCAATGTAGA TGATGATGAA TTAAGAGAGT ACTGTATTCA AGCCTTTGAA 
TCATTTGTAA GAAGATGTCC TAAGGAAGTA TATCCTCATG TTTCTACCAT TATAAATATT 
TGTCTTAAAT ATCTTACCTA TGATCCAAAT TATAATTACG ATGATGAAGA TGAAGATGAA 
AATGCAATGG ATGCTGATGG TGGTGATGAT GATGATCAAG GGAGTGATGA TGAATACAGT 
GATGATGATG ACATGAGTTG GAAAGTGAGA CGTGCAGCTG CGAAGTGCTT GGATGCTGTA 
GTTAGCACAA GGCATGAAAT GCTTCCAGAA TTCTACAAGA CCGTCTCTCC TGCACTAATA 
TCCAGATTTA AAGAGCGTGA AGAGAATGTA AAGGCAGATG TTTTTCACGC ATACCTTTCT 
CTTTTGAAGC AAACTCGTCC TGTACAAAGT TGGCTATGTG ACCCTGATGC AATGGAGCAG 
GGAGAAACAC CTTTAACAAT GCTTCAGAGT CAGGTTCCCA ACATTGTTAA AGCTCTTCAC 
AAACAGATGA AAGAAAAAAG TGTGAAGACC CGACAGTGTT GTTTTAACAT GTTAACTGAG 
CTGGTAAATG TATTACCTGG GGCCCTAACT CAACACATTC CTGTACTTGT ACCAGGAATC 
ATTTTCTCAC TGAATGATAA ATCAAGCTCA TCGAATTTGA AGATCGATGC TTTGTCATGT 
CTATACGTAA TCCTCTGTAA CCATTCTCCT CAAGTCTTCC ATCCTCACGT TCAGGCTTTG 
GTTCCTCCAG TGGTGGCTTG TGTTGGAGAC CCATTTTACA AAATTACATC TGAAGCACTT 
CTTGTTACTC AACAGCTTGT CAAAGTAATT CGTCCTTTAG ATCAGCCTTC CTCGTTTGAT 
GCAACTCCTT ATATCAAAGA TCTATTTACC TGTACCATTA AGAGATTAAA AGCAGCTGAC 
ATTGATCAGG AAGTCAAGGA AAGGGCTATT TCCTGTATGG GACAAATTAT TTGCAACCTT 
GGAGACAATT TGGGTTCTGA CTTGCCTAAT ACACTTCAGA TTTTCTTGGA GAGACTAAAG 
AATGAAATTA CCAGGTTAAC TACAGTAAAG GCATTGACAC TGATTGCTGG GTCACCTTTG 
AAGATAGATT TGAGGCCTGT TCTGGGAGAA GGGGTTCCTA TCCTTGCTTC ATTTCTTAGA 
AAAAACCAGA GAGCTTTGAA ACTGGGTACT CTTTCTGCCC TTGATATTCT AATAAAAAAC 
TATAGTGACA GCTTGACAGC TGCCATGATT GATGCAGTTC TAGATGAGCT CCCACCTCTT 
ATCAGCGAAA GTGATATGCA TGTTTCACAA ATGGCCATCA GTTTTCTTAC CACTTTGGCA 
AAAGTATATC CCTCCTCCCT TTCAAAGATA AGTGGATCCA TTCTCAATGA ACTTATTGGA 
CTTGTGAGAT CACCCTTATT GCAGGGGGGA GCTCTTAGTG CCATGCTAGA CTTTTTCCAA 
GCTCTGGTTG TCACTGGAAC AAATAATTTA GGATACATGG ATTTGTTGCG CATGCTGACT 
GGTCCAGTTT ACTCTCAGAG CACAGCTCTT ACTCATAAGC AGTCTTATTA TTCCATTGCC 
AAATGTGTAG CTGCCCTTAC TCGAGCATGC CCTAAAGAGG GACCAGCTGT AGTAGGTCAG 
TTTATTCAAG ATGTCAAGAA CTCAAGGTCT ACAGATTCCA TTCGTCTCTT AGCTCTACTT 
TCTCTTGGAG AAGTTGGGCA TCATATTGAC TTAAGTGGAC AGTTGGAACT AAAATCTGTA 
ATACTAGAAG CTTTCTCATC TCCTAGTGAA GAAGTCAAAT CAGCTGCATC CTATGCATTA 
GGCAGCATTA GTGTGGGCAA CCTTCCTGAA TATCTGCCGT TTGTCCTGCA AGAAATAACT 
AGTCAACCCA AAAGGCAGTA TCTTTTACTT CATTCCTTGA AGGAAATTAT TAGCTCTGCA 
TCAGTGGTGG GCCTTAAACC ATATGTTGAA AACATCTGGG CCTTATTACT AAAGCACTGT 
GAGTGTGCAG AGGAAGGAAC CAGAAATGTT GTTGCTGAAT GTCTAGGAAA ACTCACTCTA 
ATTGATCCAG AAACTCTCCT TCCACGGCTT AAGGGGTACT TGATATCAGG CTCATCATAT 
GCCCGAAGCT CAGTGGTTAC GGCTGTGAAA TTTACAATTT CTGACCATCC ACAACCTATT 
GATCCACTGT TAAAGAACTG CATAGGTGAT TTCCTAAAAA CTTTGGAAGA CCCAGATTTG 
AATGTGAGAA GAGTAGCCTT GGTCACATTT AATTCAGCAG CACATAACAA GCCATCATTA 
ATAAGGGATC TATTGGATAC TGTTCTTCCA CATCTTTACA ATGAAACAAA AGTTAGAAAG 
GAGCTTATAA GAGAGGTAGA AATGGGTCCA TTTAAACATA CGGTTGATGA TGGTCTGGAT 
ATTAGAAAGG CAGCATTTGA GTGTATGTAC ACACTTCTAG ACAGTTGTCT TGATAGACTT 
GATATCTTTG AATTTCTAAA TCATGTTGAA GATGGTTTGA AGGACCATTA TGATATTAAG 
ATGCTGACAT TTTTAATGTT GGTGAGACTG TCTACCCTTT GTCCAAGTGC AGTACTGCAG 
AGGTTGGACC GACTTGTTGA GCCATTACGT GCAACATGTA CAACTAAGGT AAAGGCAAAC 
TCAGTAAAGC AGGAGTTTGA AAAACAAGAT GAATTAAAGC GATCTGCCAT GAGAGCAGTA 
GCAGCACTGC TAACCATTCC AGAAGCAGAG AAGAGTCCAC TGATGAGTGA ATTCCAGTCA 
CAGATCAGTT CTAACCCTGA GCTGGCGGCT ATCTTTGAAA GTATCCAGAA AGATTCATCA 
TCTACTAACT TGGAATCAAT GGACACTAGT TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

miR-33a inhibits cell proliferation and invasion by targeting CAND1 in lung cancer.
Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico(2017 Sep)
Kang M,Li Y,Zhao Y,He S,Shi J

Disclosing the Interaction of Carbonic Anhydrase IX with Cullin-Associated NEDD8-Dissociated Protein 1 by Molecular Modeling and Integrated Binding Measurements.
ACS chemical biology12(6)1460-1465(2017 Jun)
Buonanno M,Langella E,Zambrano N,Succoio M,Sasso E,Alterio V,Di Fiore A,Sandomenico A,Supuran CT,Scaloni A,Monti SM,De Simone G

CAND1 exchange factor promotes Keap1 integration into cullin 3-RING ubiquitin ligase during adipogenesis.
The international journal of biochemistry & cell biology6695-100(2015 Sep)
Dubiel D,Ordemann J,Pratschke J,Dubiel W,Naumann M

Cand1 promotes assembly of new SCF complexes through dynamic exchange of F box proteins.
Cell153(1)206-15(2013 Mar)
Pierce NW,Lee JE,Liu X,Sweredoski MJ,Graham RL,Larimore EA,Rome M,Zheng N,Clurman BE,Hess S,Shan SO,Deshaies RJ

COMMD1 (copper metabolism MURR1 domain-containing protein 1) regulates Cullin RING ligases by preventing CAND1 (Cullin-associated Nedd8-dissociated protein 1) binding.
The Journal of biological chemistry286(37)32355-65(2011 Sep)
Mao X,Gluck N,Chen B,Starokadomskyy P,Li H,Maine GN,Burstein E

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CAND1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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