MBD5 cDNA ORF clone, Homo sapiens(human)

The following MBD5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MBD5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu19437 NM_018328.4
Latest version!
Homo sapiens methyl-CpG binding domain protein 5 (MBD5), mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $727.30
$1039.00
OHu36432 XM_017004482.1
Latest version!
Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu36432 XM_024452987.1
Latest version!
Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu69742 XM_011511470.2
Latest version!
Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu69742 XM_011511472.2
Latest version!
Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu69743 XM_017004483.1
Latest version!
Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu69743 XM_024452988.1
Latest version!
Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu69744 XM_024452989.1
Latest version!
Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu69744 XM_017004484.1
Latest version!
Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $951.30
$1359.00
OHu69745 XM_017004485.1
Latest version!
Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00
OHu69745 XM_024452990.1
Latest version!
Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $811.30
$1159.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu19437
    Clone ID Related Accession (Same CDS sequence) NM_018328.4
    Accession Version NM_018328.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4485bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-11-15
    Organism Homo sapiens(human)
    Product methyl-CpG-binding domain protein 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DA240785.1, EF542797.1, AB040894.1 and CA449443.1. This sequence is a reference standard in the RefSeqGene project. On or before Aug 31, 2013 this sequence version replaced XM_005263713.1, NM_018328.3. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: EF542797.1, BC150264.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMEA1968968, SAMEA2148874 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
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    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC
    CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG
    CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA
    ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA
    ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA
    CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA
    CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA
    GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG
    CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG
    AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC
    AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT
    TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT
    GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG
    CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC
    CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG
    CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA
    GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA
    GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA
    TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT
    TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT
    AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT
    GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA
    AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT
    GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG
    AAGGTGGAGC CCGAGAAGTT GAAGACACTA ACAGAAGGTT TGGAAGCCTA CAGCCGTGTC
    CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA AATAACCATT TAGAAGCTGC TATTCATGAG
    GCCATGAGTG AACTGGACAA AATGTCTGGG ACTGTACACC AAATCCCACA GGGTGACAGA
    CAAATGAGAC CCCCCAAACC CAAGAGGAGG AAGATCTCCA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060798.2
    CDS998..5482
    Translation

    Target ORF information:

    RefSeq Version NM_018328.4
    Organism Homo sapiens(human)
    Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018328.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC
    CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG
    CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA
    ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA
    ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA
    CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA
    CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA
    GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG
    CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG
    AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC
    AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT
    TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT
    GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG
    CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC
    CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG
    CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA
    GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA
    GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA
    TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT
    TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT
    AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT
    GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA
    AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT
    GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG
    AAGGTGGAGC CCGAGAAGTT GAAGACACTA ACAGAAGGTT TGGAAGCCTA CAGCCGTGTC
    CGGAAAAGGA ACAGAAAAAG TGGAAAGCTA AATAACCATT TAGAAGCTGC TATTCATGAG
    GCCATGAGTG AACTGGACAA AATGTCTGGG ACTGTACACC AAATCCCACA GGGTGACAGA
    CAAATGAGAC CCCCCAAACC CAAGAGGAGG AAGATCTCCA GATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36432
    Clone ID Related Accession (Same CDS sequence) XM_017004482.1 , XM_024452987.1
    Accession Version XM_017004482.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5184bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product methyl-CpG-binding domain protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG
    AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT
    GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA
    ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC
    AAGAGGAGGA AGATCTCCAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016859971.1
    CDS680..5863
    Translation

    Target ORF information:

    RefSeq Version XM_017004482.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004482.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG
    AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT
    GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA
    ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC
    AAGAGGAGGA AGATCTCCAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu36432
    Clone ID Related Accession (Same CDS sequence) XM_017004482.1 , XM_024452987.1
    Accession Version XM_024452987.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5184bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product methyl-CpG-binding domain protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG
    AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT
    GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA
    ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC
    AAGAGGAGGA AGATCTCCAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308755.1
    CDS313..5496
    Translation

    Target ORF information:

    RefSeq Version XM_024452987.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452987.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG
    AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT
    GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA
    ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC
    AAGAGGAGGA AGATCTCCAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69742
    Clone ID Related Accession (Same CDS sequence) XM_011511470.2 , XM_011511472.2
    Accession Version XM_011511470.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511470.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT
    GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG
    GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA
    GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTACACCAA
    ATCCCACAGG GTGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011509772.1
    CDS1230..6452
    Translation

    Target ORF information:

    RefSeq Version XM_011511470.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011511470.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT
    GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG
    GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA
    GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTACACCAA
    ATCCCACAGG GTGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69742
    Clone ID Related Accession (Same CDS sequence) XM_011511470.2 , XM_011511472.2
    Accession Version XM_011511472.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5223bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product methyl-CpG-binding domain protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011511472.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT
    GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG
    GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA
    GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTACACCAA
    ATCCCACAGG GTGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_011509774.1
    CDS810..6032
    Translation

    Target ORF information:

    RefSeq Version XM_011511472.2
    Organism Homo sapiens(human)
    Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_011511472.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT
    GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG
    GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAAAGTG GAAAGCTAAA TAACCATTTA
    GAAGCTGCTA TTCATGAGGC CATGAGTGAA CTGGACAAAA TGTCTGGGAC TGTACACCAA
    ATCCCACAGG GTGACAGACA AATGAGACCC CCCAAACCCA AGAGGAGGAA GATCTCCAGA
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69743
    Clone ID Related Accession (Same CDS sequence) XM_024452988.1 , XM_017004483.1
    Accession Version XM_024452988.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product methyl-CpG-binding domain protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT
    GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG
    GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAACTGA GGGGAAGAAG AAAAGAGGAA
    AAGGCTGAAA AAGGAAGGTG TCATTTGGAG CAAGATAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308756.1
    CDS313..5454
    Translation

    Target ORF information:

    RefSeq Version XM_024452988.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452988.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT
    GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG
    GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAACTGA GGGGAAGAAG AAAAGAGGAA
    AAGGCTGAAA AAGGAAGGTG TCATTTGGAG CAAGATAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69743
    Clone ID Related Accession (Same CDS sequence) XM_024452988.1 , XM_017004483.1
    Accession Version XM_017004483.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5142bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product methyl-CpG-binding domain protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT
    GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG
    GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAACTGA GGGGAAGAAG AAAAGAGGAA
    AAGGCTGAAA AAGGAAGGTG TCATTTGGAG CAAGATAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016859972.1
    CDS680..5821
    Translation

    Target ORF information:

    RefSeq Version XM_017004483.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004483.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTCTGGGT AATGTGGTTT
    GGTCTTCATA CCTTCACTCA GGTGGAGCCC GAGAAGTTGA AGACACTAAC AGAAGGTTTG
    GAAGCCTACA GCCGTGTCCG GAAAAGGAAC AGAAAACTGA GGGGAAGAAG AAAAGAGGAA
    AAGGCTGAAA AAGGAAGGTG TCATTTGGAG CAAGATAATT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69744
    Clone ID Related Accession (Same CDS sequence) XM_017004484.1 , XM_024452989.1
    Accession Version XM_017004484.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5103bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product methyl-CpG-binding domain protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG
    AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAACTG
    AGGGGAAGAA GAAAAGAGGA AAAGGCTGAA AAAGGAAGGT GTCATTTGGA GCAAGATAAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016859973.1
    CDS680..5782
    Translation

    Target ORF information:

    RefSeq Version XM_017004484.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004484.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG
    AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAACTG
    AGGGGAAGAA GAAAAGAGGA AAAGGCTGAA AAAGGAAGGT GTCATTTGGA GCAAGATAAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69744
    Clone ID Related Accession (Same CDS sequence) XM_017004484.1 , XM_024452989.1
    Accession Version XM_024452989.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5103bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product methyl-CpG-binding domain protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG
    AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAACTG
    AGGGGAAGAA GAAAAGAGGA AAAGGCTGAA AAAGGAAGGT GTCATTTGGA GCAAGATAAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308757.1
    CDS313..5415
    Translation

    Target ORF information:

    RefSeq Version XM_024452989.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452989.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGCAAG TCTGAGATCA ACCTCCACCC TTTAGGTTTT
    CTCAACCCGA ATGTAAACGC TGCTTTAGCT TTTCTCTCCA GTGACATGGA TGGGCAGGTA
    TTGCAGCCTG TTCACTTTCA GCTCTTAGCA GCCTTGCTTC AGAACCAAGC CCAAGCAGCT
    GCCATGCTTC CCCTGCCATC TTTCAATCTG ACCATCTCAG ATCTTTTGCA ACAGCAAAAT
    ACCCCTTTAC CCTCATTAAC ACAGATGACA GCCCCACCAG ACCATTTGCC AAGCAATCAG
    TCAGACAACA GCCGAGCTGA GACCCTTTTA ACCAGCCCCC TGGGGAACCC TTTACCAAGC
    TTTGCAGGCA GTGACACTAC TTTTAACCCC CTGTTCCTCC CAGCTGTCAA TGGGGCCTCA
    GGATTAATGA CCTTGAATCC CCAGCTGTTG GGAGGTGTCC TGAACTCGGC ATCGGCCAAC
    ACCGCTAATC ATCCAGAGGT TTCCATAGCA ACCTCCTCCC AGGCAACCAC TACCACAACC
    ACTACATCAT CAGCAGTGGC AGCACTGACT GTCTCAACAC TTGGTGGGAC AGCAGTGGTG
    TCAATGGCAG AAACATTGCT GAATATATCT AATAATGCTG GGAATACACC TGGTCCAGCT
    AAACTCAACA GTAACTCTGT GGTGCCACAG CTACTTAACC CTCTACTGGG GACAGGTCTA
    CTTGGTGATA TGTCATCAAT AAACAATACT TTGAGTAACC ATCAACTGAC TCATCTACAG
    TCGCTGTTAA ACAACAATCA GATGTTTCCT CCAAATCAGC AACAGCAGCA ACTTCTCCAG
    GGGTACCAGA ATCTCCAGGC GTTCCAAGGA CAGTCCACAA TTCCTTGCCC AGCTAACAAT
    AACCCCATGG CTTGTCTGTT TCAGAACTTT CAGGTGAGAA TGCAGGAAGA TGCAGCTCTC
    CTAAACAAAA GAATAAGCAC TCAGCCTGGG CTCACAGCAC TTCCTGAGAA TCCAAACACT
    ACACTTCCAC CTTTTCAAGA TACACCTTGT GAGTTGCAAC CGAGGATTGA CCCATCTCTT
    GGTCAACAGG TGAAGGATGG CCTCGTTGTG GGTGGCCCAG GTGATGCTTC CGTAGATGCC
    ATTTACAAAG CAGTTGTCGA TGCAGCCAGC AAAGGAATGC AGGTTGTCAT CACCACTGCA
    GTCAACAGTA CAACTCAGAT CAGCCCCATT CCAGCTCTGA GTGCCATGAG TGCCTTCACT
    GCCTCAATTG GTGACCCATT AAATCTCTCC AGTGCTGTCA GTGCGGTCAT TCATGGACGG
    AACATGGGAG GTGTTGATCA TGATGGTAGG CTGAGGAATT CAAGAGGGGC TCGGCTGCCC
    AAGAATCTAG ACCATGGGAA AAATGTGAAC GAAGGAGATG GGTTTGAATA TTTCAAGTCA
    GCAAGTTGCC ACACATCCAA AAAACAGTGG GACGGGGAGC AAAGCCCCAG AGGGGAGCGA
    AACAGGTGGA AGTACGAGGA ATTTTTAGAT CATCCAGGCC ATATCCACAG TAGTCCTTGT
    CATGAAAGGC CCAACAATGT CTCTACACTG CCATTTCTGC CTGGGGAACA GCACCCAATA
    CTGTTACCAC CAAGAAACTG TCCAGGGGAT AAAATTCTAG AGGAAAATTT CAGGTATAAT
    AACTACAAAA GAACTATGAT GAGTTTTAAG GAGAGACTAG AGAACACTGT GGAAAGATGT
    GCACACATAA ATGGGAATAG ACCTCGACAG AGTCGGGGAT TTGGAGAGCT GCTAAGCACT
    GCAAAGCAAG ACCTGGTCCT AGAGGAGCAG TCTCCAAGTT CCTCAAATAG TTTGGAAAAT
    TCTCTGGTCA AAGACTACAT CCATTACAAT GGAGACTTTA ATGCCAAAAG CGTTAATGGG
    TGTGTGCCTA GCCCTTCAGA TGCTAAAAGC ATTAGTAGTG AAGATGACCT AAGGAACCCA
    GACTCCCCCT CTTCAAATGA ATTGATACAT TATAGACCAA GGACGTTCAA TGTTGGCGAC
    TTGGTCTGGG GCCAAATCAA AGGACTGACT TCCTGGCCTG GAAAATTAGT AAGAGAAGAC
    GACGTTCACA ATTCATGTCA ACAAAGCCCC GAGGAAGGGA AGGTGGAGCC CGAGAAGTTG
    AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAACTG
    AGGGGAAGAA GAAAAGAGGA AAAGGCTGAA AAAGGAAGGT GTCATTTGGA GCAAGATAAT
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69745
    Clone ID Related Accession (Same CDS sequence) XM_024452990.1 , XM_017004485.1
    Accession Version XM_024452990.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-05
    Organism Homo sapiens(human)
    Product methyl-CpG-binding domain protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC
    CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG
    CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA
    ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA
    ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA
    CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA
    CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA
    GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG
    CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG
    AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC
    AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT
    TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT
    GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG
    CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC
    CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG
    CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA
    GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA
    GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA
    TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT
    TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT
    AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT
    GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA
    AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT
    GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG
    AAGGTCTGGG TAATGTGGTT TGGTCTTCAT ACCTTCACTC AGGTGGAGCC CGAGAAGTTG
    AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT
    GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA
    ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC
    AAGAGGAGGA AGATCTCCAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024308758.1
    CDS313..4836
    Translation

    Target ORF information:

    RefSeq Version XM_024452990.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024452990.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC
    CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG
    CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA
    ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA
    ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA
    CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA
    CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA
    GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG
    CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG
    AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC
    AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT
    TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT
    GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG
    CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC
    CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG
    CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA
    GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA
    GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA
    TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT
    TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT
    AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT
    GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA
    AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT
    GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG
    AAGGTCTGGG TAATGTGGTT TGGTCTTCAT ACCTTCACTC AGGTGGAGCC CGAGAAGTTG
    AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT
    GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA
    ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC
    AAGAGGAGGA AGATCTCCAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu69745
    Clone ID Related Accession (Same CDS sequence) XM_024452990.1 , XM_017004485.1
    Accession Version XM_017004485.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4524bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-05
    Organism Homo sapiens(human)
    Product methyl-CpG-binding domain protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000002.12) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC
    CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG
    CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA
    ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA
    ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA
    CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA
    CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA
    GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG
    CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG
    AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC
    AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT
    TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT
    GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG
    CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC
    CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG
    CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA
    GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA
    GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA
    TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT
    TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT
    AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT
    GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA
    AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT
    GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG
    AAGGTCTGGG TAATGTGGTT TGGTCTTCAT ACCTTCACTC AGGTGGAGCC CGAGAAGTTG
    AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT
    GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA
    ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC
    AAGAGGAGGA AGATCTCCAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_016859974.1
    CDS680..5203
    Translation

    Target ORF information:

    RefSeq Version XM_017004485.1
    Organism Homo sapiens(human)
    Definition Homo sapiens methyl-CpG binding domain protein 5 (MBD5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017004485.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAATGGAG GCAAAGAGTG TGACGGAGGG GACAAGGAAG GAGGTCTTCC AGCTATACAA 
    GTTCCTGTGG GTTGGCAGCG TCGTGTGGAT CAAAATGGAG TGCTTTATGT CAGTCCCAGT
    GGGTCTTTGT TATCTTGCTT GGAGCAGGTT AAAACATACC TGCTTACTGA TGGAACATGC
    AAGTGTGGCT TGGAATGTCC TCTTATTCTT CCCAAGGTAT TTAATTTTGA TCCTGGAGCT
    GCTGTGAAAC AGAGAACCGC AGAAGATGTT AAGGCAGATG AAGATGTCAC AAAGCTATGC
    ATACATAAAA GAAAAATTAT TGCAGTGGCC ACACTTCATA AAAGCATGGA AGCCCCACAT
    CCTTCTCTGG TGCTCACCAG TCCCGGAGGA GGAACAAATG CAACTCCAGT AGTACCTTCT
    CGGGCAGCAA CTCCAAGATC AGTAAGAAAT AAGTCTCATG AAGGAATTAC AAATTCTGTA
    ATGCCTGAAT GTAAGAATCC TTTCAAGTTA ATGATTGGAT CATCAAATGC CATGGGAAGG
    CTATATGTAC AAGAACTGCC TGGAAGCCAA CAACAAGAAC TCCACCCTGT CTACCCCCGA
    CAGAGATTGG GCAGCAGTGA ACATGGACAG AAATCTCCAT TCCGTGGCAG CCATGGAGGC
    CTGCCCAGCC CAGCGTCATC AGGTTCCCAG ATATATGGAG ATGGTTCAAT CTCTCCAAGG
    ACTGACCCAC TTGGAAGTCC TGATGTTTTC ACAAGAAGTA ATCCTGGTTT TCATGGAGCT
    CCCAATTCTA GTCCTATTCA CCTGAATAGG ACTCCTCTTT CTCCACCTTC AGTAATGCTA
    CATGGTTCTC CTGTACAGTC ATCCTGTGCA ATGGCTGGAA GGACTAATAT ACCTCTTTCC
    CCAACCTTGA CTACAAAGAG TCCAGTAATG AAAAAACCAA TGTGTAATTT TTCAACTAAT
    ATGGAAATAC CACGAGCAAT GTTCCACCAC AAACCACCCC AAGGCCCACC TCCCCCTCCT
    CCACCTTCTT GTGCTCTTCA GAAAAAGCCA TTAACATCTG AGAAAGATCC ACTTGGCATT
    CTTGACCCTA TTCCTAGTAA ACCAGTGAAT CAGAACCCTG TTATCATTAA TCCAACCAGT
    TTCCATTCAA ATGTCCACTC TCAGGTACCT ATGATGAATG TAAGCATGCC TCCTGCTGTT
    GTTCCTTTGC CAAGTAATCT CCCATTGCCA ACTGTAAAAC CTGGTCACAT GAATCATGGG
    AGTCATGTAC AAAGAGTTCA GCATTCAGCT TCAACCTCCC TGTCCCCTTC TCCAGTGACA
    TCCCCCGTGC ACATGATGGG GACTGGAATT GGAAGGATTG AGGCATCGCC CCAAAGATCA
    CGCTCATCTT CCACATCATC AGATCATGGA AATTTCATGA TGCCACCTGT AGGACCCCAG
    GCCACTTCTA GTGGTATTAA GGTTCCACCC AGGTCACCAA GGTCAACAAT AGGGTCCCCA
    AGGCCATCAA TGCCATCAAG CCCTTCTACC AAGTCCGATG GACATCATCA GTACAAGGAT
    ATCCCTAACC CATTAATTGC TGGAATAAGT AATGTACTAA ATACCCCAAG CAGTGCAGCT
    TTTCCTACTG CATCTGCCGG AAGTAGTTCT GTAAAGAGTC AGCCTGGTTT GCTGGGAATG
    CCTTTAAATC AGATCTTGAA CCAGCACAAT GCTGCCTCCT TTCCAGCAAG TAGTTTACTC
    TCAGCAGCAG CCAAAGCACA GCTAGCAAAT CAAAACAAAC TTGCTGGTAA CAACAGTAGC
    AGCAGTAGCA ATTCTGGAGC TGTTGCCGGC AGTGGCAACA CTGAAGGACA TAGCACTTTA
    AACACCATGT TCCCTCCTAC TGCCAACATG CTTCTCCCAA CAGGTGAAGG GCAAAGTGGT
    CGAGCAGCAC TAAGAGATAA GCTGATGTCT CAGCAAAAAG ACGCATTGCG GAAAAGAAAA
    CAACCACCTA CGACAGTGTT GAGTTTGCTC AGACAGTCTC AAATGGATAG TTCTGCAGTT
    CCTAAACCTG GACCTGACTT GCTAAGGAAG CAGGGTCAGG GTTCATTTCC CATCAGTTCA
    ATGTCTCAGT TACTACAGTC TATGAGTTGT CAAAGCTCTC ACTTGAGTAG CAATAGTACC
    CCGGGTTGTG GGGCCTCAAA TACTGCTTTG CCTTGCTCTG CTAACCAGCT GCATTTTACA
    GATCCCAGTA TGAACTCTAG TGTTCTTCAG AACATACCTT TAAGAGGGGA AGCCGTGCAC
    TGCCACAATG CAAACACTAA CTTTGTTCAC AGTAACAGTC CAGTCCCCAA CCACCATCTT
    GCAGGTTTAA TAAATCAGAT TCAGGCTAGC GGGAACTGTG GGATGCTCAG TCAGTCGGGC
    ATGGCTTTAG GAAATTCCTT ACATCCCAAT CCACCTCAGT CAAGAATTTC AACGTCCTCC
    ACTCCAGTGA TACCAAACAG CATTGTTAGC AGCTATAATC AAACAAGTTC TGAAGCAGGC
    GGTTCAGGAC CATCATCCTC CATAGCCATA GCGGGCACCA ACCACCCTGC CATCACAAAG
    ACAACATCTG TTCTTCAAGA TGGCGTCATA GTCACCACTG CAGCTGGAAA CCCACTGCAG
    AGTCAGCTAC CCATTGGGAG TGATTTTCCT TTTGTTGGCC AGGAGCACGC ACTTCATTTT
    CCATCCAACA GCACTTCAAA CAACCATCTT CCACACCCCT TGAACCCCAG CCTCCTCAGT
    TCTCTACCTA TCTCTTTGCC AGTGAATCAA CAGCATCTCC TAAACCAGAA TCTATTAAAT
    ATCCTCCAGC CTTCAGCAGG AGAAGGTGAT ATGTCATCAA TAAACAATAC TTTGAGTAAC
    CATCAACTGA CTCATCTACA GTCGCTGTTA AACAACAATC AGATGTTTCC TCCAAATCAG
    CAACAGCAGC AACTTCTCCA GGGGTACCAG AATCTCCAGG CGTTCCAAGG ACAGTCCACA
    ATTCCTTGCC CAGCTAACAA TAACCCCATG GCTTGTCTGT TTCAGAACTT TCAGGTGAGA
    ATGCAGGAAG ATGCAGCTCT CCTAAACAAA AGAATAAGCA CTCAGCCTGG GCTCACAGCA
    CTTCCTGAGA ATCCAAACAC TACACTTCCA CCTTTTCAAG ATACACCTTG TGAGTTGCAA
    CCGAGGATTG ACCCATCTCT TGGTCAACAG GTGAAGGATG GCCTCGTTGT GGGTGGCCCA
    GGTGATGCTT CCGTAGATGC CATTTACAAA GCAGTTGTCG ATGCAGCCAG CAAAGGAATG
    CAGGTTGTCA TCACCACTGC AGTCAACAGT ACAACTCAGA TCAGCCCCAT TCCAGCTCTG
    AGTGCCATGA GTGCCTTCAC TGCCTCAATT GGTGACCCAT TAAATCTCTC CAGTGCTGTC
    AGTGCGGTCA TTCATGGACG GAACATGGGA GGTGTTGATC ATGATGGTAG GCTGAGGAAT
    TCAAGAGGGG CTCGGCTGCC CAAGAATCTA GACCATGGGA AAAATGTGAA CGAAGGAGAT
    GGGTTTGAAT ATTTCAAGTC AGCAAGTTGC CACACATCCA AAAAACAGTG GGACGGGGAG
    CAAAGCCCCA GAGGGGAGCG AAACAGGTGG AAGTACGAGG AATTTTTAGA TCATCCAGGC
    CATATCCACA GTAGTCCTTG TCATGAAAGG CCCAACAATG TCTCTACACT GCCATTTCTG
    CCTGGGGAAC AGCACCCAAT ACTGTTACCA CCAAGAAACT GTCCAGGGGA TAAAATTCTA
    GAGGAAAATT TCAGGTATAA TAACTACAAA AGAACTATGA TGAGTTTTAA GGAGAGACTA
    GAGAACACTG TGGAAAGATG TGCACACATA AATGGGAATA GACCTCGACA GAGTCGGGGA
    TTTGGAGAGC TGCTAAGCAC TGCAAAGCAA GACCTGGTCC TAGAGGAGCA GTCTCCAAGT
    TCCTCAAATA GTTTGGAAAA TTCTCTGGTC AAAGACTACA TCCATTACAA TGGAGACTTT
    AATGCCAAAA GCGTTAATGG GTGTGTGCCT AGCCCTTCAG ATGCTAAAAG CATTAGTAGT
    GAAGATGACC TAAGGAACCC AGACTCCCCC TCTTCAAATG AATTGATACA TTATAGACCA
    AGGACGTTCA ATGTTGGCGA CTTGGTCTGG GGCCAAATCA AAGGACTGAC TTCCTGGCCT
    GGAAAATTAG TAAGAGAAGA CGACGTTCAC AATTCATGTC AACAAAGCCC CGAGGAAGGG
    AAGGTCTGGG TAATGTGGTT TGGTCTTCAT ACCTTCACTC AGGTGGAGCC CGAGAAGTTG
    AAGACACTAA CAGAAGGTTT GGAAGCCTAC AGCCGTGTCC GGAAAAGGAA CAGAAAAAGT
    GGAAAGCTAA ATAACCATTT AGAAGCTGCT ATTCATGAGG CCATGAGTGA ACTGGACAAA
    ATGTCTGGGA CTGTACACCA AATCCCACAG GGTGACAGAC AAATGAGACC CCCCAAACCC
    AAGAGGAGGA AGATCTCCAG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.