CHFR cDNA ORF clone, Homo sapiens(human)

The following CHFR gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CHFR cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu15061 NM_001161344.1
Latest version!
Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$219.50-$307.30
$439.00
OHu22042 NM_018223.2
Latest version!
Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$49.50-$69.30
$99.00
OHu15152 NM_001161346.1
Latest version!
Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu15331 NM_001161345.1
Latest version!
Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu15351 NM_001161347.1
Latest version!
Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $307.30
$439.00
OHu109451 NM_001161346.2
Latest version!
Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $307.30
$439.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu15061
    Clone ID Related Accession (Same CDS sequence) NM_001161344.1
    Accession Version NM_001161344.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1995bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase CHFR isoform 1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP313700.1, AF170724.1, AC127070.10 and BC012072.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.193923.1, AF170724.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
    CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC
    GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT
    GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT
    GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGAAGCTAA CAAGGAAAAT
    GTGTTCCATG GGACCAAAGA TACCTCAGGT GCAGGTGCAG GGCGAGGGGC CGATCCCCGG
    GTCCCTCCGT CGTCGCCCGC CACTCAGGTG TGCTTTGAGG AACCACAGCC ATCAACATCG
    ACGTCAGACC TCTTCCCCAC AGCCTCGGCC TCTTCCACGG AGCCTTCTCC TGCAGGGCGA
    GAGCGTTCCT CCAGTTGTGG GTCTGGGGGT GGTGGCATCT CCCCTAAAGG AAGTGGTCCC
    TCTGTGGCAA GTGATGAAGT CTCCAGCTTT GCCTCAGCTC TCCCAGACAG AAAGACTGCG
    TCCTTTTCGT CGTTGGAACC CCAGGATCAG GAGGATTTGG AGCCCGTGAA GAAGAAAATG
    AGAGGAGATG GGGACCTTGA CCTGAACGGG CAGTTGTTGG TCGCACAACC GCGTAGAAAT
    GCCCAAACCG TCCACGAGGA CGTCAGAGCA GCGGCTGGGA AGCCAGACAA GATGGAGGAG
    ACGCTGACAT GCATCATCTG CCAGGACCTG CTGCACGACT GCGTGAGTTT GCAGCCCTGC
    ATGCACACGT TCTGCGCGGC TTGCTACTCG GGCTGGATGG AGCGCTCGTC CCTGTGTCCT
    ACCTGCCGCT GTCCCGTGGA GCGGATCTGT AAAAACCACA TCCTCAACAA CCTCGTGGAA
    GCATACCTCA TCCAGCATCC AGACAAGAGT CGCAGTGAAG AAGATGTGCA AAGTATGGAT
    GCCAGGAATA AAATCACTCA AGACATGCTG CAGCCCAAAG TCAGGCGGTC TTTTTCTGAT
    GAAGAAGGGA GTTCAGAGGA CCTGCTGGAG CTGTCAGACG TTGACAGTGA GTCCTCAGAC
    ATTAGCCAGC CATACGTCGT GTGCCGGCAG TGTCCTGAGT ACAGAAGGCA GGCGGCGCAG
    CCTCCCCACT GCCCAGCACC CGAGGGCGAG CCAGGAGCCC CACAGGCCCT GGGGGATGCA
    CCCTCCACGT CCGTCAGCCT GACGACAGCA GTCCAGGATT ACGTGTGCCC TCTGCAAGGA
    AGCCACGCCC TGTGCACCTG CTGCTTCCAG CCCATGCCCG ACCGGAGAGC GGAGCGCGAG
    CAGGACCCGC GTGTCGCCCC TCAGCAGTGT GCGGTCTGCC TGCAGCCTTT CTGCCACCTG
    TACTGGGGCT GCACCCGGAC CGGCTGCTAC GGCTGCCTGG CCCCGTTTTG TGAGCTCAAC
    CTGGGTGACA AGTGTCTGGA CGGCGTGCTG AACAACAACA GCTACGAGTC AGACATCCTG
    AAGAATTACC TGGCAACCAG AGGTTTGACA TGGAAAAACA TGTTGACCGA GAGCCTCGTG
    GCTCTCCAGC GGGGAGTGTT TCTGCTGTCT GATTACAGAG TCACGGGAGA CACCGTTCTG
    TGTTACTGCT GTGGCCTGCG CAGCTTCCGT GAGCTGACCT ATCAGTATCG GCAGAACATT
    CCTGCTTCCG AGTTGCCAGT GGCCGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC
    TGCCGCACTC AGGTGAAAGC TCACCACGCC ATGAAATTCA ATCATATCTG TGAACAGACA
    AGGTTCAAAA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001154816.1
    CDS85..2079
    Translation

    Target ORF information:

    RefSeq Version NM_001161344.1
    Organism Homo sapiens(human)
    Definition Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001161344.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
    CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC
    GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT
    GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT
    GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGAAGCTAA CAAGGAAAAT
    GTGTTCCATG GGACCAAAGA TACCTCAGGT GCAGGTGCAG GGCGAGGGGC CGATCCCCGG
    GTCCCTCCGT CGTCGCCCGC CACTCAGGTG TGCTTTGAGG AACCACAGCC ATCAACATCG
    ACGTCAGACC TCTTCCCCAC AGCCTCGGCC TCTTCCACGG AGCCTTCTCC TGCAGGGCGA
    GAGCGTTCCT CCAGTTGTGG GTCTGGGGGT GGTGGCATCT CCCCTAAAGG AAGTGGTCCC
    TCTGTGGCAA GTGATGAAGT CTCCAGCTTT GCCTCAGCTC TCCCAGACAG AAAGACTGCG
    TCCTTTTCGT CGTTGGAACC CCAGGATCAG GAGGATTTGG AGCCCGTGAA GAAGAAAATG
    AGAGGAGATG GGGACCTTGA CCTGAACGGG CAGTTGTTGG TCGCACAACC GCGTAGAAAT
    GCCCAAACCG TCCACGAGGA CGTCAGAGCA GCGGCTGGGA AGCCAGACAA GATGGAGGAG
    ACGCTGACAT GCATCATCTG CCAGGACCTG CTGCACGACT GCGTGAGTTT GCAGCCCTGC
    ATGCACACGT TCTGCGCGGC TTGCTACTCG GGCTGGATGG AGCGCTCGTC CCTGTGTCCT
    ACCTGCCGCT GTCCCGTGGA GCGGATCTGT AAAAACCACA TCCTCAACAA CCTCGTGGAA
    GCATACCTCA TCCAGCATCC AGACAAGAGT CGCAGTGAAG AAGATGTGCA AAGTATGGAT
    GCCAGGAATA AAATCACTCA AGACATGCTG CAGCCCAAAG TCAGGCGGTC TTTTTCTGAT
    GAAGAAGGGA GTTCAGAGGA CCTGCTGGAG CTGTCAGACG TTGACAGTGA GTCCTCAGAC
    ATTAGCCAGC CATACGTCGT GTGCCGGCAG TGTCCTGAGT ACAGAAGGCA GGCGGCGCAG
    CCTCCCCACT GCCCAGCACC CGAGGGCGAG CCAGGAGCCC CACAGGCCCT GGGGGATGCA
    CCCTCCACGT CCGTCAGCCT GACGACAGCA GTCCAGGATT ACGTGTGCCC TCTGCAAGGA
    AGCCACGCCC TGTGCACCTG CTGCTTCCAG CCCATGCCCG ACCGGAGAGC GGAGCGCGAG
    CAGGACCCGC GTGTCGCCCC TCAGCAGTGT GCGGTCTGCC TGCAGCCTTT CTGCCACCTG
    TACTGGGGCT GCACCCGGAC CGGCTGCTAC GGCTGCCTGG CCCCGTTTTG TGAGCTCAAC
    CTGGGTGACA AGTGTCTGGA CGGCGTGCTG AACAACAACA GCTACGAGTC AGACATCCTG
    AAGAATTACC TGGCAACCAG AGGTTTGACA TGGAAAAACA TGTTGACCGA GAGCCTCGTG
    GCTCTCCAGC GGGGAGTGTT TCTGCTGTCT GATTACAGAG TCACGGGAGA CACCGTTCTG
    TGTTACTGCT GTGGCCTGCG CAGCTTCCGT GAGCTGACCT ATCAGTATCG GCAGAACATT
    CCTGCTTCCG AGTTGCCAGT GGCCGTAACA TCCCGTCCTG ACTGCTACTG GGGCCGTAAC
    TGCCGCACTC AGGTGAAAGC TCACCACGCC ATGAAATTCA ATCATATCTG TGAACAGACA
    AGGTTCAAAA ACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu22042
    Clone ID Related Accession (Same CDS sequence) NM_018223.2
    Accession Version NM_018223.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1872bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-01
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase CHFR isoform 4
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP313700.1, AK001658.1, AK027687.1, AC127070.10 and BC012072.1. This sequence is a reference standard in the RefSeqGene project. On Jun 6, 2009 this sequence version replaced NM_018223.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK001658.1, SRR1660809.224931.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
    CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC
    GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT
    GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT
    GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGAGATGGT GCCTTGCTGT
    GTTGCCCAGG CTGGTCTAAA ACTCCTGGGA TCAAGTGATC CTCCCACCTT GGCCTCCCAA
    AGTATTGTGA TTACAGGGTC TGGGGGTGGT GGCATCTCCC CTAAAGGAAG TGGTCCCTCT
    GTGGCAAGTG ATGAAGTCTC CAGCTTTGCC TCAGCTCTCC CAGACAGAAA GACTGCGTCC
    TTTTCGTCGT TGGAACCCCA GGATCAGGAG GATTTGGAGC CCGTGAAGAA GAAAATGAGA
    GGAGATGGGG ACCTTGACCT GAACGGGCAG TTGTTGGTCG CACAACCGCG TAGAAATGCC
    CAAACCGTCC ACGAGGACGT CAGAGCAGCG GCTGGGAAGC CAGACAAGAT GGAGGAGACG
    CTGACATGCA TCATCTGCCA GGACCTGCTG CACGACTGCG TGAGTTTGCA GCCCTGCATG
    CACACGTTCT GCGCGGCTTG CTACTCGGGC TGGATGGAGC GCTCGTCCCT GTGTCCTACC
    TGCCGCTGTC CCGTGGAGCG GATCTGTAAA AACCACATCC TCAACAACCT CGTGGAAGCA
    TACCTCATCC AGCATCCAGA CAAGAGTCGC AGTGAAGAAG ATGTGCAAAG TATGGATGCC
    AGGAATAAAA TCACTCAAGA CATGCTGCAG CCCAAAGTCA GGCGGTCTTT TTCTGATGAA
    GAAGGGAGTT CAGAGGACCT GCTGGAGCTG TCAGACGTTG ACAGTGAGTC CTCAGACATT
    AGCCAGCCAT ACGTCGTGTG CCGGCAGTGT CCTGAGTACA GAAGGCAGGC GGCGCAGCCT
    CCCCACTGCC CAGCACCCGA GGGCGAGCCA GGAGCCCCAC AGGCCCTGGG GGATGCACCC
    TCCACGTCCG TCAGCCTGAC GACAGCAGTC CAGGATTACG TGTGCCCTCT GCAAGGAAGC
    CACGCCCTGT GCACCTGCTG CTTCCAGCCC ATGCCCGACC GGAGAGCGGA GCGCGAGCAG
    GACCCGCGTG TCGCCCCTCA GCAGTGTGCG GTCTGCCTGC AGCCTTTCTG CCACCTGTAC
    TGGGGCTGCA CCCGGACCGG CTGCTACGGC TGCCTGGCCC CGTTTTGTGA GCTCAACCTG
    GGTGACAAGT GTCTGGACGG CGTGCTGAAC AACAACAGCT ACGAGTCAGA CATCCTGAAG
    AATTACCTGG CAACCAGAGG TTTGACATGG AAAAACATGT TGACCGAGAG CCTCGTGGCT
    CTCCAGCGGG GAGTGTTTCT GCTGTCTGAT TACAGAGTCA CGGGAGACAC CGTTCTGTGT
    TACTGCTGTG GCCTGCGCAG CTTCCGTGAG CTGACCTATC AGTATCGGCA GAACATTCCT
    GCTTCCGAGT TGCCAGTGGC CGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGC
    CGCACTCAGG TGAAAGCTCA CCACGCCATG AAATTCAATC ATATCTGTGA ACAGACAAGG
    TTCAAAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_060693.2
    CDS85..1956
    Translation

    Target ORF information:

    RefSeq Version NM_018223.2
    Organism Homo sapiens(human)
    Definition Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_018223.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
    CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC
    GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT
    GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT
    GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGAGATGGT GCCTTGCTGT
    GTTGCCCAGG CTGGTCTAAA ACTCCTGGGA TCAAGTGATC CTCCCACCTT GGCCTCCCAA
    AGTATTGTGA TTACAGGGTC TGGGGGTGGT GGCATCTCCC CTAAAGGAAG TGGTCCCTCT
    GTGGCAAGTG ATGAAGTCTC CAGCTTTGCC TCAGCTCTCC CAGACAGAAA GACTGCGTCC
    TTTTCGTCGT TGGAACCCCA GGATCAGGAG GATTTGGAGC CCGTGAAGAA GAAAATGAGA
    GGAGATGGGG ACCTTGACCT GAACGGGCAG TTGTTGGTCG CACAACCGCG TAGAAATGCC
    CAAACCGTCC ACGAGGACGT CAGAGCAGCG GCTGGGAAGC CAGACAAGAT GGAGGAGACG
    CTGACATGCA TCATCTGCCA GGACCTGCTG CACGACTGCG TGAGTTTGCA GCCCTGCATG
    CACACGTTCT GCGCGGCTTG CTACTCGGGC TGGATGGAGC GCTCGTCCCT GTGTCCTACC
    TGCCGCTGTC CCGTGGAGCG GATCTGTAAA AACCACATCC TCAACAACCT CGTGGAAGCA
    TACCTCATCC AGCATCCAGA CAAGAGTCGC AGTGAAGAAG ATGTGCAAAG TATGGATGCC
    AGGAATAAAA TCACTCAAGA CATGCTGCAG CCCAAAGTCA GGCGGTCTTT TTCTGATGAA
    GAAGGGAGTT CAGAGGACCT GCTGGAGCTG TCAGACGTTG ACAGTGAGTC CTCAGACATT
    AGCCAGCCAT ACGTCGTGTG CCGGCAGTGT CCTGAGTACA GAAGGCAGGC GGCGCAGCCT
    CCCCACTGCC CAGCACCCGA GGGCGAGCCA GGAGCCCCAC AGGCCCTGGG GGATGCACCC
    TCCACGTCCG TCAGCCTGAC GACAGCAGTC CAGGATTACG TGTGCCCTCT GCAAGGAAGC
    CACGCCCTGT GCACCTGCTG CTTCCAGCCC ATGCCCGACC GGAGAGCGGA GCGCGAGCAG
    GACCCGCGTG TCGCCCCTCA GCAGTGTGCG GTCTGCCTGC AGCCTTTCTG CCACCTGTAC
    TGGGGCTGCA CCCGGACCGG CTGCTACGGC TGCCTGGCCC CGTTTTGTGA GCTCAACCTG
    GGTGACAAGT GTCTGGACGG CGTGCTGAAC AACAACAGCT ACGAGTCAGA CATCCTGAAG
    AATTACCTGG CAACCAGAGG TTTGACATGG AAAAACATGT TGACCGAGAG CCTCGTGGCT
    CTCCAGCGGG GAGTGTTTCT GCTGTCTGAT TACAGAGTCA CGGGAGACAC CGTTCTGTGT
    TACTGCTGTG GCCTGCGCAG CTTCCGTGAG CTGACCTATC AGTATCGGCA GAACATTCCT
    GCTTCCGAGT TGCCAGTGGC CGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGC
    CGCACTCAGG TGAAAGCTCA CCACGCCATG AAATTCAATC ATATCTGTGA ACAGACAAGG
    TTCAAAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15152
    Clone ID Related Accession (Same CDS sequence) NM_001161346.1
    Accession Version NM_001161346.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-05-03
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase CHFR isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP313700.1, AK027687.1, AC127070.10 and BC012072.1. Transcript Variant: This variant (3) uses an alternate in-frame splice site in the 5' coding region compared to variant 1. This results in a shorter protein (isoform 3) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.125085.1, SRR1803613.158.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
    CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC
    GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT
    GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT
    GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGATACCTC AGGTGCAGGT
    GCAGGGCGAG GGGCCGATCC CCGGGTCCCT CCGTCGTCGC CCGCCACTCA GGTGTGCTTT
    GAGGAACCAC AGCCATCAAC ATCGACGTCA GACCTCTTCC CCACAGCCTC GGCCTCTTCC
    ACGGAGCCTT CTCCTGCAGG GCGAGAGCGT TCCTCCAGTT GTGGGTCTGG GGGTGGTGGC
    ATCTCCCCTA AAGGAAGTGG TCCCTCTGTG GCAAGTGATG AAGTCTCCAG CTTTGCCTCA
    GCTCTCCCAG ACAGAAAGAC TGCGTCCTTT TCGTCGTTGG AACCCCAGGA TCAGGAGGAT
    TTGGAGCCCG TGAAGAAGAA AATGAGAGGA GATGGGGACC TTGACCTGAA CGGGCAGTTG
    TTGGTCGCAC AACCGCGTAG AAATGCCCAA ACCGTCCACG AGGACGTCAG AGCAGCGGCT
    GGGAAGCCAG ACAAGATGGA GGAGACGCTG ACATGCATCA TCTGCCAGGA CCTGCTGCAC
    GACTGCGTGA GTTTGCAGCC CTGCATGCAC ACGTTCTGCG CGGCTTGCTA CTCGGGCTGG
    ATGGAGCGCT CGTCCCTGTG TCCTACCTGC CGCTGTCCCG TGGAGCGGAT CTGTAAAAAC
    CACATCCTCA ACAACCTCGT GGAAGCATAC CTCATCCAGC ATCCAGACAA GAGTCGCAGT
    GAAGAAGATG TGCAAAGTAT GGATGCCAGG AATAAAATCA CTCAAGACAT GCTGCAGCCC
    AAAGTCAGGC GGTCTTTTTC TGATGAAGAA GGGAGTTCAG AGGACCTGCT GGAGCTGTCA
    GACGTTGACA GTGAGTCCTC AGACATTAGC CAGCCATACG TCGTGTGCCG GCAGTGTCCT
    GAGTACAGAA GGCAGGCGGC GCAGCCTCCC CACTGCCCAG CACCCGAGGG CGAGCCAGGA
    GCCCCACAGG CCCTGGGGGA TGCACCCTCC ACGTCCGTCA GCCTGACGAC AGCAGTCCAG
    GATTACGTGT GCCCTCTGCA AGGAAGCCAC GCCCTGTGCA CCTGCTGCTT CCAGCCCATG
    CCCGACCGGA GAGCGGAGCG CGAGCAGGAC CCGCGTGTCG CCCCTCAGCA GTGTGCGGTC
    TGCCTGCAGC CTTTCTGCCA CCTGTACTGG GGCTGCACCC GGACCGGCTG CTACGGCTGC
    CTGGCCCCGT TTTGTGAGCT CAACCTGGGT GACAAGTGTC TGGACGGCGT GCTGAACAAC
    AACAGCTACG AGTCAGACAT CCTGAAGAAT TACCTGGCAA CCAGAGGTTT GACATGGAAA
    AACATGTTGA CCGAGAGCCT CGTGGCTCTC CAGCGGGGAG TGTTTCTGCT GTCTGATTAC
    AGAGTCACGG GAGACACCGT TCTGTGTTAC TGCTGTGGCC TGCGCAGCTT CCGTGAGCTG
    ACCTATCAGT ATCGGCAGAA CATTCCTGCT TCCGAGTTGC CAGTGGCCGT AACATCCCGT
    CCTGACTGCT ACTGGGGCCG TAACTGCCGC ACTCAGGTGA AAGCTCACCA CGCCATGAAA
    TTCAATCATA TCTGTGAACA GACAAGGTTC AAAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001154818.1
    CDS85..2043
    Translation

    Target ORF information:

    RefSeq Version NM_001161346.1
    Organism Homo sapiens(human)
    Definition Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001161346.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
    CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC
    GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT
    GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT
    GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGATACCTC AGGTGCAGGT
    GCAGGGCGAG GGGCCGATCC CCGGGTCCCT CCGTCGTCGC CCGCCACTCA GGTGTGCTTT
    GAGGAACCAC AGCCATCAAC ATCGACGTCA GACCTCTTCC CCACAGCCTC GGCCTCTTCC
    ACGGAGCCTT CTCCTGCAGG GCGAGAGCGT TCCTCCAGTT GTGGGTCTGG GGGTGGTGGC
    ATCTCCCCTA AAGGAAGTGG TCCCTCTGTG GCAAGTGATG AAGTCTCCAG CTTTGCCTCA
    GCTCTCCCAG ACAGAAAGAC TGCGTCCTTT TCGTCGTTGG AACCCCAGGA TCAGGAGGAT
    TTGGAGCCCG TGAAGAAGAA AATGAGAGGA GATGGGGACC TTGACCTGAA CGGGCAGTTG
    TTGGTCGCAC AACCGCGTAG AAATGCCCAA ACCGTCCACG AGGACGTCAG AGCAGCGGCT
    GGGAAGCCAG ACAAGATGGA GGAGACGCTG ACATGCATCA TCTGCCAGGA CCTGCTGCAC
    GACTGCGTGA GTTTGCAGCC CTGCATGCAC ACGTTCTGCG CGGCTTGCTA CTCGGGCTGG
    ATGGAGCGCT CGTCCCTGTG TCCTACCTGC CGCTGTCCCG TGGAGCGGAT CTGTAAAAAC
    CACATCCTCA ACAACCTCGT GGAAGCATAC CTCATCCAGC ATCCAGACAA GAGTCGCAGT
    GAAGAAGATG TGCAAAGTAT GGATGCCAGG AATAAAATCA CTCAAGACAT GCTGCAGCCC
    AAAGTCAGGC GGTCTTTTTC TGATGAAGAA GGGAGTTCAG AGGACCTGCT GGAGCTGTCA
    GACGTTGACA GTGAGTCCTC AGACATTAGC CAGCCATACG TCGTGTGCCG GCAGTGTCCT
    GAGTACAGAA GGCAGGCGGC GCAGCCTCCC CACTGCCCAG CACCCGAGGG CGAGCCAGGA
    GCCCCACAGG CCCTGGGGGA TGCACCCTCC ACGTCCGTCA GCCTGACGAC AGCAGTCCAG
    GATTACGTGT GCCCTCTGCA AGGAAGCCAC GCCCTGTGCA CCTGCTGCTT CCAGCCCATG
    CCCGACCGGA GAGCGGAGCG CGAGCAGGAC CCGCGTGTCG CCCCTCAGCA GTGTGCGGTC
    TGCCTGCAGC CTTTCTGCCA CCTGTACTGG GGCTGCACCC GGACCGGCTG CTACGGCTGC
    CTGGCCCCGT TTTGTGAGCT CAACCTGGGT GACAAGTGTC TGGACGGCGT GCTGAACAAC
    AACAGCTACG AGTCAGACAT CCTGAAGAAT TACCTGGCAA CCAGAGGTTT GACATGGAAA
    AACATGTTGA CCGAGAGCCT CGTGGCTCTC CAGCGGGGAG TGTTTCTGCT GTCTGATTAC
    AGAGTCACGG GAGACACCGT TCTGTGTTAC TGCTGTGGCC TGCGCAGCTT CCGTGAGCTG
    ACCTATCAGT ATCGGCAGAA CATTCCTGCT TCCGAGTTGC CAGTGGCCGT AACATCCCGT
    CCTGACTGCT ACTGGGGCCG TAACTGCCGC ACTCAGGTGA AAGCTCACCA CGCCATGAAA
    TTCAATCATA TCTGTGAACA GACAAGGTTC AAAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15331
    Clone ID Related Accession (Same CDS sequence) NM_001161345.1
    Accession Version NM_001161345.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1992bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-27
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase CHFR isoform 2
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP313700.1, AK304333.1, AK027687.1, AC127070.10 and BC012072.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK304333.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
    CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC
    GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT
    GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT
    GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGAAGCTAA CAAGGAAAAT
    GTGTTCCATG GGACCAAAGA TACCTCAGGT GCAGGTGCAG GGCGAGGGGC CGATCCCCGG
    GTCCCTCCGT CGTCGCCCGC CACTCAGGTG TGCTTTGAGG AACCACAGCC ATCAACATCG
    ACGTCAGACC TCTTCCCCAC AGCCTCGGCC TCTTCCACGG AGCCTTCTCC TGCAGGGCGA
    GAGCGTTCCT CCAGTTGTGG GTCTGGGGGT GGTGGCATCT CCCCTAAAGG AAGTGGTCCC
    TCTGTGGCAA GTGATGAAGT CTCCAGCTTT GCCTCAGCTC TCCCAGACAG AAAGACTGCG
    TCCTTTTCGT CGTTGGAACC CCAGGATCAG GAGGATTTGG AGCCCGTGAA GAAGAAAATG
    AGAGGAGATG GGGACCTTGA CCTGAACGGG CAGTTGTTGG TCGCACAACC GCGTAGAAAT
    GCCCAAACCG TCCACGAGGA CGTCAGAGCA GCGGCTGGGA AGCCAGACAA GATGGAGGAG
    ACGCTGACAT GCATCATCTG CCAGGACCTG CTGCACGACT GCGTGAGTTT GCAGCCCTGC
    ATGCACACGT TCTGCGCGGC TTGCTACTCG GGCTGGATGG AGCGCTCGTC CCTGTGTCCT
    ACCTGCCGCT GTCCCGTGGA GCGGATCTGT AAAAACCACA TCCTCAACAA CCTCGTGGAA
    GCATACCTCA TCCAGCATCC AGACAAGAGT CGCAGTGAAG AAGATGTGCA AAGTATGGAT
    GCCAGGAATA AAATCACTCA AGACATGCTG CAGCCCAAAG TCAGGCGGTC TTTTTCTGAT
    GAAGAAGGGA GTTCAGAGGA CCTGCTGGAG CTGTCAGACG TTGACAGTGA GTCCTCAGAC
    ATTAGCCAGC CATACGTCGT GTGCCGGCAG TGTCCTGAGT ACAGAAGGCA GGCGGCGCAG
    CCTCCCCACT GCCCAGCACC CGAGGGCGAG CCAGGAGCCC CACAGGCCCT GGGGGATGCA
    CCCTCCACGT CCGTCAGCCT GACGACAGTC CAGGATTACG TGTGCCCTCT GCAAGGAAGC
    CACGCCCTGT GCACCTGCTG CTTCCAGCCC ATGCCCGACC GGAGAGCGGA GCGCGAGCAG
    GACCCGCGTG TCGCCCCTCA GCAGTGTGCG GTCTGCCTGC AGCCTTTCTG CCACCTGTAC
    TGGGGCTGCA CCCGGACCGG CTGCTACGGC TGCCTGGCCC CGTTTTGTGA GCTCAACCTG
    GGTGACAAGT GTCTGGACGG CGTGCTGAAC AACAACAGCT ACGAGTCAGA CATCCTGAAG
    AATTACCTGG CAACCAGAGG TTTGACATGG AAAAACATGT TGACCGAGAG CCTCGTGGCT
    CTCCAGCGGG GAGTGTTTCT GCTGTCTGAT TACAGAGTCA CGGGAGACAC CGTTCTGTGT
    TACTGCTGTG GCCTGCGCAG CTTCCGTGAG CTGACCTATC AGTATCGGCA GAACATTCCT
    GCTTCCGAGT TGCCAGTGGC CGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGC
    CGCACTCAGG TGAAAGCTCA CCACGCCATG AAATTCAATC ATATCTGTGA ACAGACAAGG
    TTCAAAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001154817.1
    CDS85..2076
    Translation

    Target ORF information:

    RefSeq Version NM_001161345.1
    Organism Homo sapiens(human)
    Definition Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001161345.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
    CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC
    GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT
    GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT
    GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGAAGCTAA CAAGGAAAAT
    GTGTTCCATG GGACCAAAGA TACCTCAGGT GCAGGTGCAG GGCGAGGGGC CGATCCCCGG
    GTCCCTCCGT CGTCGCCCGC CACTCAGGTG TGCTTTGAGG AACCACAGCC ATCAACATCG
    ACGTCAGACC TCTTCCCCAC AGCCTCGGCC TCTTCCACGG AGCCTTCTCC TGCAGGGCGA
    GAGCGTTCCT CCAGTTGTGG GTCTGGGGGT GGTGGCATCT CCCCTAAAGG AAGTGGTCCC
    TCTGTGGCAA GTGATGAAGT CTCCAGCTTT GCCTCAGCTC TCCCAGACAG AAAGACTGCG
    TCCTTTTCGT CGTTGGAACC CCAGGATCAG GAGGATTTGG AGCCCGTGAA GAAGAAAATG
    AGAGGAGATG GGGACCTTGA CCTGAACGGG CAGTTGTTGG TCGCACAACC GCGTAGAAAT
    GCCCAAACCG TCCACGAGGA CGTCAGAGCA GCGGCTGGGA AGCCAGACAA GATGGAGGAG
    ACGCTGACAT GCATCATCTG CCAGGACCTG CTGCACGACT GCGTGAGTTT GCAGCCCTGC
    ATGCACACGT TCTGCGCGGC TTGCTACTCG GGCTGGATGG AGCGCTCGTC CCTGTGTCCT
    ACCTGCCGCT GTCCCGTGGA GCGGATCTGT AAAAACCACA TCCTCAACAA CCTCGTGGAA
    GCATACCTCA TCCAGCATCC AGACAAGAGT CGCAGTGAAG AAGATGTGCA AAGTATGGAT
    GCCAGGAATA AAATCACTCA AGACATGCTG CAGCCCAAAG TCAGGCGGTC TTTTTCTGAT
    GAAGAAGGGA GTTCAGAGGA CCTGCTGGAG CTGTCAGACG TTGACAGTGA GTCCTCAGAC
    ATTAGCCAGC CATACGTCGT GTGCCGGCAG TGTCCTGAGT ACAGAAGGCA GGCGGCGCAG
    CCTCCCCACT GCCCAGCACC CGAGGGCGAG CCAGGAGCCC CACAGGCCCT GGGGGATGCA
    CCCTCCACGT CCGTCAGCCT GACGACAGTC CAGGATTACG TGTGCCCTCT GCAAGGAAGC
    CACGCCCTGT GCACCTGCTG CTTCCAGCCC ATGCCCGACC GGAGAGCGGA GCGCGAGCAG
    GACCCGCGTG TCGCCCCTCA GCAGTGTGCG GTCTGCCTGC AGCCTTTCTG CCACCTGTAC
    TGGGGCTGCA CCCGGACCGG CTGCTACGGC TGCCTGGCCC CGTTTTGTGA GCTCAACCTG
    GGTGACAAGT GTCTGGACGG CGTGCTGAAC AACAACAGCT ACGAGTCAGA CATCCTGAAG
    AATTACCTGG CAACCAGAGG TTTGACATGG AAAAACATGT TGACCGAGAG CCTCGTGGCT
    CTCCAGCGGG GAGTGTTTCT GCTGTCTGAT TACAGAGTCA CGGGAGACAC CGTTCTGTGT
    TACTGCTGTG GCCTGCGCAG CTTCCGTGAG CTGACCTATC AGTATCGGCA GAACATTCCT
    GCTTCCGAGT TGCCAGTGGC CGTAACATCC CGTCCTGACT GCTACTGGGG CCGTAACTGC
    CGCACTCAGG TGAAAGCTCA CCACGCCATG AAATTCAATC ATATCTGTGA ACAGACAAGG
    TTCAAAAACT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu15351
    Clone ID Related Accession (Same CDS sequence) NM_001161347.1
    Accession Version NM_001161347.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1719bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-01
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase CHFR isoform 5
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BP313700.1, AK302785.1, AK027687.1, AC127070.10 and BC012072.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK302785.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
    CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC
    GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT
    GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAGGTCTGG GGGTGGTGGC
    ATCTCCCCTA AAGGAAGTGG TCCCTCTGTG GCAAGTGATG AAGTCTCCAG CTTTGCCTCA
    GCTCTCCCAG ACAGAAAGAC TGCGTCCTTT TCGTCGTTGG AACCCCAGGA TCAGGAGGAT
    TTGGAGCCCG TGAAGAAGAA AATGAGAGGA GATGGGGACC TTGACCTGAA CGGGCAGTTG
    TTGGTCGCAC AACCGCGTAG AAATGCCCAA ACCGTCCACG AGGACGTCAG AGCAGCGGCT
    GGGAAGCCAG ACAAGATGGA GGAGACGCTG ACATGCATCA TCTGCCAGGA CCTGCTGCAC
    GACTGCGTGA GTTTGCAGCC CTGCATGCAC ACGTTCTGCG CGGCTTGCTA CTCGGGCTGG
    ATGGAGCGCT CGTCCCTGTG TCCTACCTGC CGCTGTCCCG TGGAGCGGAT CTGTAAAAAC
    CACATCCTCA ACAACCTCGT GGAAGCATAC CTCATCCAGC ATCCAGACAA GAGTCGCAGT
    GAAGAAGATG TGCAAAGTAT GGATGCCAGG AATAAAATCA CTCAAGACAT GCTGCAGCCC
    AAAGTCAGGC GGTCTTTTTC TGATGAAGAA GGGAGTTCAG AGGACCTGCT GGAGCTGTCA
    GACGTTGACA GTGAGTCCTC AGACATTAGC CAGCCATACG TCGTGTGCCG GCAGTGTCCT
    GAGTACAGAA GGCAGGCGGC GCAGCCTCCC CACTGCCCAG CACCCGAGGG CGAGCCAGGA
    GCCCCACAGG CCCTGGGGGA TGCACCCTCC ACGTCCGTCA GCCTGACGAC AGCAGTCCAG
    GATTACGTGT GCCCTCTGCA AGGAAGCCAC GCCCTGTGCA CCTGCTGCTT CCAGCCCATG
    CCCGACCGGA GAGCGGAGCG CGAGCAGGAC CCGCGTGTCG CCCCTCAGCA GTGTGCGGTC
    TGCCTGCAGC CTTTCTGCCA CCTGTACTGG GGCTGCACCC GGACCGGCTG CTACGGCTGC
    CTGGCCCCGT TTTGTGAGCT CAACCTGGGT GACAAGTGTC TGGACGGCGT GCTGAACAAC
    AACAGCTACG AGTCAGACAT CCTGAAGAAT TACCTGGCAA CCAGAGGTTT GACATGGAAA
    AACATGTTGA CCGAGAGCCT CGTGGCTCTC CAGCGGGGAG TGTTTCTGCT GTCTGATTAC
    AGAGTCACGG GAGACACCGT TCTGTGTTAC TGCTGTGGCC TGCGCAGCTT CCGTGAGCTG
    ACCTATCAGT ATCGGCAGAA CATTCCTGCT TCCGAGTTGC CAGTGGCCGT AACATCCCGT
    CCTGACTGCT ACTGGGGCCG TAACTGCCGC ACTCAGGTGA AAGCTCACCA CGCCATGAAA
    TTCAATCATA TCTGTGAACA GACAAGGTTC AAAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001154819.1
    CDS85..1803
    Translation

    Target ORF information:

    RefSeq Version NM_001161347.1
    Organism Homo sapiens(human)
    Definition Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001161347.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
    CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC
    GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT
    GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAGGTCTGG GGGTGGTGGC
    ATCTCCCCTA AAGGAAGTGG TCCCTCTGTG GCAAGTGATG AAGTCTCCAG CTTTGCCTCA
    GCTCTCCCAG ACAGAAAGAC TGCGTCCTTT TCGTCGTTGG AACCCCAGGA TCAGGAGGAT
    TTGGAGCCCG TGAAGAAGAA AATGAGAGGA GATGGGGACC TTGACCTGAA CGGGCAGTTG
    TTGGTCGCAC AACCGCGTAG AAATGCCCAA ACCGTCCACG AGGACGTCAG AGCAGCGGCT
    GGGAAGCCAG ACAAGATGGA GGAGACGCTG ACATGCATCA TCTGCCAGGA CCTGCTGCAC
    GACTGCGTGA GTTTGCAGCC CTGCATGCAC ACGTTCTGCG CGGCTTGCTA CTCGGGCTGG
    ATGGAGCGCT CGTCCCTGTG TCCTACCTGC CGCTGTCCCG TGGAGCGGAT CTGTAAAAAC
    CACATCCTCA ACAACCTCGT GGAAGCATAC CTCATCCAGC ATCCAGACAA GAGTCGCAGT
    GAAGAAGATG TGCAAAGTAT GGATGCCAGG AATAAAATCA CTCAAGACAT GCTGCAGCCC
    AAAGTCAGGC GGTCTTTTTC TGATGAAGAA GGGAGTTCAG AGGACCTGCT GGAGCTGTCA
    GACGTTGACA GTGAGTCCTC AGACATTAGC CAGCCATACG TCGTGTGCCG GCAGTGTCCT
    GAGTACAGAA GGCAGGCGGC GCAGCCTCCC CACTGCCCAG CACCCGAGGG CGAGCCAGGA
    GCCCCACAGG CCCTGGGGGA TGCACCCTCC ACGTCCGTCA GCCTGACGAC AGCAGTCCAG
    GATTACGTGT GCCCTCTGCA AGGAAGCCAC GCCCTGTGCA CCTGCTGCTT CCAGCCCATG
    CCCGACCGGA GAGCGGAGCG CGAGCAGGAC CCGCGTGTCG CCCCTCAGCA GTGTGCGGTC
    TGCCTGCAGC CTTTCTGCCA CCTGTACTGG GGCTGCACCC GGACCGGCTG CTACGGCTGC
    CTGGCCCCGT TTTGTGAGCT CAACCTGGGT GACAAGTGTC TGGACGGCGT GCTGAACAAC
    AACAGCTACG AGTCAGACAT CCTGAAGAAT TACCTGGCAA CCAGAGGTTT GACATGGAAA
    AACATGTTGA CCGAGAGCCT CGTGGCTCTC CAGCGGGGAG TGTTTCTGCT GTCTGATTAC
    AGAGTCACGG GAGACACCGT TCTGTGTTAC TGCTGTGGCC TGCGCAGCTT CCGTGAGCTG
    ACCTATCAGT ATCGGCAGAA CATTCCTGCT TCCGAGTTGC CAGTGGCCGT AACATCCCGT
    CCTGACTGCT ACTGGGGCCG TAACTGCCGC ACTCAGGTGA AAGCTCACCA CGCCATGAAA
    TTCAATCATA TCTGTGAACA GACAAGGTTC AAAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu109451
    Clone ID Related Accession (Same CDS sequence) NM_001161346.2
    Accession Version NM_001161346.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1959bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2020-01-04
    Organism Homo sapiens(human)
    Product E3 ubiquitin-protein ligase CHFR isoform 3
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC127070.10. On Aug 2, 2019 this sequence version replaced NM_001161346.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803617.125085.1, SRR1803615.188338.1 [ECO:0000332] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
    CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC
    GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT
    GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT
    GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGATACCTC AGGTGCAGGT
    GCAGGGCGAG GGGCCGATCC CCGGGTCCCT CCGTCGTCGC CCGCCACTCA GGTGTGCTTT
    GAGGAACCAC AGCCATCAAC ATCGACGTCA GACCTCTTCC CCACAGCCTC GGCCTCTTCC
    ACGGAGCCTT CTCCTGCAGG GCGAGAGCGT TCCTCCAGTT GTGGGTCTGG GGGTGGTGGC
    ATCTCCCCTA AAGGAAGTGG TCCCTCTGTG GCAAGTGATG AAGTCTCCAG CTTTGCCTCA
    GCTCTCCCAG ACAGAAAGAC TGCGTCCTTT TCGTCGTTGG AACCCCAGGA TCAGGAGGAT
    TTGGAGCCCG TGAAGAAGAA AATGAGAGGA GATGGGGACC TTGACCTGAA CGGGCAGTTG
    TTGGTCGCAC AACCGCGTAG AAATGCCCAA ACCGTCCACG AGGACGTCAG AGCAGCGGCT
    GGGAAGCCAG ACAAGATGGA GGAGACGCTG ACATGCATCA TCTGCCAGGA CCTGCTGCAC
    GACTGCGTGA GTTTGCAGCC CTGCATGCAC ACGTTCTGCG CGGCTTGCTA CTCGGGCTGG
    ATGGAGCGCT CGTCCCTGTG TCCTACCTGC CGCTGTCCCG TGGAGCGGAT CTGTAAAAAC
    CACATCCTCA ACAACCTCGT GGAAGCATAC CTCATCCAGC ATCCAGACAA GAGTCGCAGT
    GAAGAAGATG TGCAAAGTAT GGATGCCAGA AATAAAATCA CTCAAGACAT GCTGCAGCCC
    AAAGTCAGGC GGTCTTTTTC TGATGAAGAA GGGAGTTCAG AGGACCTGCT GGAGCTGTCA
    GACGTTGACA GTGAGTCCTC AGACATTAGC CAGCCATACG TCGTGTGCCG GCAGTGTCCT
    GAGTACAGAA GGCAGGCGGC GCAGCCTCCC CACTGCCCAG CACCCGAGGG CGAGCCAGGA
    GCCCCACAGG CCCTGGGGGA TGCACCCTCC ACGTCCGTCA GCCTGACGAC AGCAGTCCAG
    GATTACGTGT GCCCTCTGCA AGGAAGCCAC GCCCTGTGCA CCTGCTGCTT CCAGCCCATG
    CCCGACCGGA GAGCGGAGCG CGAGCAGGAC CCGCGTGTCG CCCCTCAGCA GTGTGCGGTC
    TGCCTGCAGC CTTTCTGCCA CCTGTACTGG GGCTGCACCC GGACCGGCTG CTACGGCTGC
    CTGGCCCCGT TTTGTGAGCT CAACCTGGGT GACAAGTGTC TGGACGGCGT GCTGAACAAC
    AACAGCTACG AGTCAGACAT CCTGAAGAAT TACCTGGCAA CCAGAGGTTT GACATGGAAA
    AACATGTTGA CCGAGAGCCT CGTGGCTCTC CAGCGGGGAG TGTTTCTGCT GTCTGATTAC
    AGAGTCACGG GAGACACCGT TCTGTGTTAC TGCTGTGGCC TGCGCAGCTT CCGTGAGCTG
    ACCTATCAGT ATCGGCAGAA CATTCCTGCT TCCGAGTTGC CAGTGGCCGT AACATCCCGT
    CCTGACTGCT ACTGGGGCCG TAACTGCCGC ACTCAGGTGA AAGCTCACCA CGCCATGAAA
    TTCAATCATA TCTGTGAACA GACAAGGTTC AAAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001154818.1
    CDS72..2030
    Translation

    Target ORF information:

    RefSeq Version NM_001161346.2
    Organism Homo sapiens(human)
    Definition Homo sapiens checkpoint with forkhead and ring finger domains (CHFR), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001161346.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    ATGGAGCGGC CCGAGGAAGG CAAGCAGTCG CCGCCGCCGC AGCCCTGGGG ACGGCTCCTG 
    CGTCTGGGCG CGGAGGAGGG CGAGCCGCAC GTCCTCCTGA GGAAGCGGGA GTGGACCATC
    GGGCGGAGAC GAGGTTGCGA CCTTTCCTTC CCCAGCAATA AACTGGTCTC TGGAGATCAC
    TGTAGAATTG TAGTGGATGA AAAATCAGGT CAGGTGACAC TGGAAGATAC CAGCACCAGT
    GGAACAGTGA TTAACAAGCT GAAGGTTGTT AAGAAGCAGA CATGCCCTTT ACAGACTGGG
    GATGTCATCT ACTTGGTGTA CAGGAAGAAT GAACCGGAAC ACAACGTGGC ATACCTCTAT
    GAATCTTTAA GTGAAAAGCA AGGCATGACA CAAGAATCCT TTGATACCTC AGGTGCAGGT
    GCAGGGCGAG GGGCCGATCC CCGGGTCCCT CCGTCGTCGC CCGCCACTCA GGTGTGCTTT
    GAGGAACCAC AGCCATCAAC ATCGACGTCA GACCTCTTCC CCACAGCCTC GGCCTCTTCC
    ACGGAGCCTT CTCCTGCAGG GCGAGAGCGT TCCTCCAGTT GTGGGTCTGG GGGTGGTGGC
    ATCTCCCCTA AAGGAAGTGG TCCCTCTGTG GCAAGTGATG AAGTCTCCAG CTTTGCCTCA
    GCTCTCCCAG ACAGAAAGAC TGCGTCCTTT TCGTCGTTGG AACCCCAGGA TCAGGAGGAT
    TTGGAGCCCG TGAAGAAGAA AATGAGAGGA GATGGGGACC TTGACCTGAA CGGGCAGTTG
    TTGGTCGCAC AACCGCGTAG AAATGCCCAA ACCGTCCACG AGGACGTCAG AGCAGCGGCT
    GGGAAGCCAG ACAAGATGGA GGAGACGCTG ACATGCATCA TCTGCCAGGA CCTGCTGCAC
    GACTGCGTGA GTTTGCAGCC CTGCATGCAC ACGTTCTGCG CGGCTTGCTA CTCGGGCTGG
    ATGGAGCGCT CGTCCCTGTG TCCTACCTGC CGCTGTCCCG TGGAGCGGAT CTGTAAAAAC
    CACATCCTCA ACAACCTCGT GGAAGCATAC CTCATCCAGC ATCCAGACAA GAGTCGCAGT
    GAAGAAGATG TGCAAAGTAT GGATGCCAGA AATAAAATCA CTCAAGACAT GCTGCAGCCC
    AAAGTCAGGC GGTCTTTTTC TGATGAAGAA GGGAGTTCAG AGGACCTGCT GGAGCTGTCA
    GACGTTGACA GTGAGTCCTC AGACATTAGC CAGCCATACG TCGTGTGCCG GCAGTGTCCT
    GAGTACAGAA GGCAGGCGGC GCAGCCTCCC CACTGCCCAG CACCCGAGGG CGAGCCAGGA
    GCCCCACAGG CCCTGGGGGA TGCACCCTCC ACGTCCGTCA GCCTGACGAC AGCAGTCCAG
    GATTACGTGT GCCCTCTGCA AGGAAGCCAC GCCCTGTGCA CCTGCTGCTT CCAGCCCATG
    CCCGACCGGA GAGCGGAGCG CGAGCAGGAC CCGCGTGTCG CCCCTCAGCA GTGTGCGGTC
    TGCCTGCAGC CTTTCTGCCA CCTGTACTGG GGCTGCACCC GGACCGGCTG CTACGGCTGC
    CTGGCCCCGT TTTGTGAGCT CAACCTGGGT GACAAGTGTC TGGACGGCGT GCTGAACAAC
    AACAGCTACG AGTCAGACAT CCTGAAGAAT TACCTGGCAA CCAGAGGTTT GACATGGAAA
    AACATGTTGA CCGAGAGCCT CGTGGCTCTC CAGCGGGGAG TGTTTCTGCT GTCTGATTAC
    AGAGTCACGG GAGACACCGT TCTGTGTTAC TGCTGTGGCC TGCGCAGCTT CCGTGAGCTG
    ACCTATCAGT ATCGGCAGAA CATTCCTGCT TCCGAGTTGC CAGTGGCCGT AACATCCCGT
    CCTGACTGCT ACTGGGGCCG TAACTGCCGC ACTCAGGTGA AAGCTCACCA CGCCATGAAA
    TTCAATCATA TCTGTGAACA GACAAGGTTC AAAAACTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.