VPS35 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	VPS35
Entrez Gene ID	55737
Full Name	VPS35, retromer complex component
Synonyms	MEM3,PARK17
General protein information	Preferred Names VPS35, retromer complex component Names vacuolar protein sorting-associated protein 35 hVPS35 maternal-embryonic 3 vacuolar protein sorting 35 homolog
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 16 Map Location: 16q11.2
Summary	This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq, Jul 2008].
Disorder MIM:	601501
Disorder Html:	{Parkinson disease 17}, 614203 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_018206.5	NP_060676.2	vacuolar protein sorting-associated protein 35
XM_005256045.3	XP_005256102.1	vacuolar protein sorting-associated protein 35 isoform X2
XM_011523227.2	XP_011521529.1	vacuolar protein sorting-associated protein 35 isoform X1
NM_018206.4	NP_060676.2	vacuolar protein sorting-associated protein 35
NM_018206.6	NP_060676.2	vacuolar protein sorting-associated protein 35
XM_011523227.3	XP_011521529.1	vacuolar protein sorting-associated protein 35 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	VPS35	NP_060676.2
Canis lupus familiaris (dog)	VPS35	XP_532570.3
Eremothecium gossypii	AGOS_AEL107W	NP_984754.1
Arabidopsis thaliana (thale cress)	VPS35B	NP_177713.3
Rattus norvegicus (Norway rat)	Vps35	NP_001099188.2
Danio rerio (zebrafish)	vps35	NP_001020688.2
	VPS35	XP_001108828.2
Gallus gallus (chicken)	VPS35	NP_001005842.1
Caenorhabditis elegans (roundworm)	vps-35	NP_495180.2
Saccharomyces cerevisiae (baker's yeast)	VPS35	NP_012381.1
Kluyveromyces lactis	KLLA0B07535g	XP_451864.1
Magnaporthe oryzae (rice blast fungus)	MGG_05089	XP_003712611.1
Bos taurus (cattle)	VPS35	NP_001039723.1
Drosophila melanogaster (fruit fly)	Vps35	NP_611651.4
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007683	XP_308188.4
Schizosaccharomyces pombe (fission yeast)	vps35	NP_588260.2
Oryza sativa (rice)	Os03g0801600	NP_001051593.1
Pan troglodytes (chimpanzee)	VPS35	XP_001161536.1
Mus musculus (house mouse)	Vps35	NP_075373.1
Neurospora crassa	NCU06284	XP_962546.1

Gene Ontology
Bibliography

Related articles in PubMed

Parkinson's disease-associated pathogenic VPS35 mutation causes complex I deficits.
Zhou L, Wang W, Hoppel C, Liu J, Zhu X
Biochimica et biophysica acta1863(11)2791-2795(2017 Nov)

Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.
McNally KE, Faulkner R, Steinberg F, Gallon M, Ghai R, Pim D, Langton P, Pearson N, Danson CM, N?gele H, Morris LL, Singla A, Overlee BL, Heesom KJ, Sessions R, Banks L, Collins BM, Berger I, Billadeau DD, Burstein E, Cullen PJ
Nature cell biology19(10)1214-1225(2017 Oct)

Architecture of the human interactome defines protein communities and disease networks.
Huttlin EL, Bruckner RJ, Paulo JA, Cannon JR, Ting L, Baltier K, Colby G, Gebreab F, Gygi MP, Parzen H, Szpyt J, Tam S, Zarraga G, Pontano-Vaites L, Swarup S, White AE, Schweppe DK, Rad R, Erickson BK, Obar RA, Guruharsha KG, Li K, Artavanis-Tsakonas S, Gygi SP, Harper JW
Nature545(7655)505-509(2017 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings provide new mechanistic insights into the role of VPS35 in the regulation of AIMP2 levels and cell death.
Title: VPS35 regulates parkin substrate AIMP2 toxicity by facilitating lysosomal clearance of AIMP2.

results lend further support to the notion that VPS35-DLP1 interaction is key to the retromer-dependent recycling of mitochondrial DLP1 complex during mitochondrial fission and provide a novel therapeutic target to control excessive fission and associated mitochondrial deficits.
Title: A conserved retromer sorting motif is essential for mitochondrial DLP1 recycling by VPS35 in Parkinson's disease model.

X-ray crystallographic analysis of a 4-component complex comprising the VPS26 & VPS35 subunits of retromer, sorting nexin SNX3, & recycling signal from the divalent cation transporter DMT1-II; analysis identifies a binding site for canonical recycling signals at the interface between VPS26 & SNX3; shows cooperative interactions among the VPS subunits, SNX3 & cargo that couple signal-recognition to membrane recruitment.
Title: Structural Mechanism for Cargo Recognition by the Retromer Complex.

The absence of mutations in VPS35 genes reveals that they are uncommon causes of Parkinson disease in Brazil
Title: Autosomal dominant Parkinson's disease: Incidence of mutations in LRRK2, SNCA, VPS35 and GBA genes in Brazil.

Silencing VPS35 increased N-Ras's association with cytoplasmic vesicles, diminished GTP loading of Ras, and inhibited mitogen-activated protein kinase signaling and growth of N-Ras-dependent melanoma cells.
Title: VPS35 binds farnesylated N-Ras in the cytosol to regulate N-Ras trafficking.

The following VPS35 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPS35 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu18510	NM_018206.5 Latest version!	Homo sapiens VPS35 retromer complex component (VPS35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu49428	XM_005256045.3 Latest version!	Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu69690	XM_011523227.3 Latest version!	Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
OHu108594	NM_018206.6 Latest version!	Homo sapiens VPS35 retromer complex component (VPS35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00
View Old Accession Versions >>
OHu18510	NM_018206.4	Homo sapiens VPS35 retromer complex component (VPS35), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$189.50-$265.30 $379.00
OHu69690	XM_011523227.2	Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$391.30 $559.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPS35 ( NM_018206.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_060676.2 Homo sapiens VPS35 retromer complex component (VPS35), mRNA.

Price & Availability↑

CloneID	OHu18510
Clone ID Related Accession (Same CDS sequence)	NM_018206.5 , NM_018206.4
Accession Version	NM_018206.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-24
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 35
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB049686.1, BC093036.1, BQ574179.1, AC012186.8 and BU621624.1. This sequence is a reference standard in the RefSeqGene project. On Apr 15, 2016 this sequence version replaced NM_018206.4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660805.1800.1, SRR1803613.283710.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG 
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA 
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC 
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT 
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG 
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG 
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC 
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG 
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG 
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG 
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG 
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA 
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT 
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT 
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG 
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA 
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA 
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC 
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA 
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT 
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG 
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA 
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT 
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT 
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT 
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT 
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT 
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC 
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG 
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT 
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC 
TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT 
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC 
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA 
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT 
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT 
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT 
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG 
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060676.2
CDS	100..2490
Translation	MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL

Target ORF information:

RefSeq Version	NM_018206.5
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS35 retromer complex component (VPS35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018206.5

ORF Insert Sequence:

ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG 
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA 
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC 
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT 
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG 
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG 
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC 
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG 
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG 
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG 
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG 
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA 
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT 
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT 
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG 
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA 
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA 
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC 
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA 
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT 
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG 
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA 
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT 
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT 
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT 
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT 
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT 
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC 
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG 
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT 
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC 
TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT 
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC 
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA 
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT 
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT 
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT 
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG 
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS35 ( NM_018206.4 ) cDNA ORF clone, Homo sapiens(human) -> NP_060676.2 Homo sapiens VPS35 retromer complex component (VPS35), mRNA.

Price & Availability↑

CloneID	OHu18510
Clone ID Related Accession (Same CDS sequence)	NM_018206.5 , NM_018206.4
Accession Version	NM_018206.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-24
Organism	Homo sapiens(Human)
Product	vacuolar protein sorting-associated protein 35
Comment

ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG 
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA 
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC 
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT 
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG 
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG 
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC 
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG 
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG 
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG 
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG 
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA 
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT 
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT 
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG 
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA 
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA 
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC 
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA 
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT 
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG 
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA 
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT 
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT 
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT 
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT 
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT 
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC 
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG 
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT 
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC 
TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT 
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC 
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA 
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT 
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT 
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT 
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG 
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq

NP_060676.2

Translation

MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL

Target ORF information:

RefSeq Version	NM_018206.4
Organism	Homo sapiens(Human)
Definition	Homo sapiens VPS35 retromer complex component (VPS35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018206.4

ORF Insert Sequence:

ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG 
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA 
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC 
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT 
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG 
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG 
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC 
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG 
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG 
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG 
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG 
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA 
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT 
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT 
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG 
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA 
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA 
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC 
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA 
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT 
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG 
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA 
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT 
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT 
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT 
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT 
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT 
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC 
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG 
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT 
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC 
TTCAGTGAAG AGAATCATGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT 
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC 
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA 
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT 
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT 
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT 
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG 
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS35 ( XM_005256045.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_005256102.1 Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X2, mRNA.

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CloneID	OHu49428
Clone ID Related Accession (Same CDS sequence)	XM_005256045.3
Accession Version	XM_005256045.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2190bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 35 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_005256045.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCATTT CTGATGAACT GCACTACTTG GAGGTCTACC TGACAGATGA GTTTGCTAAA 
GGAAGGAAAG TGGCAGATCT CTACGAACTT GTACAGTATG CTGGAAACAT TATCCCAAGG 
CTTTACCTTT TGATCACAGT TGGAGTTGTA TATGTCAAGT CATTTCCTCA GTCCAGGAAG 
GATATTTTGA AAGATTTGGT AGAAATGTGC CGTGGTGTGC AACATCCCTT GAGGGGTCTG 
TTTCTTCGAA ATTACCTTCT TCAGTGTACC AGAAATATCT TACCTGATGA AGGAGAGCCA 
ACAGATGAAG AAACAACTGG TGACATCAGT GATTCCATGG ATTTTGTACT GCTCAACTTT 
GCAGAAATGA ACAAGCTCTG GGTGCGAATG CAGCATCAGG GACATAGCCG AGATAGAGAA 
AAAAGAGAAC GAGAAAGACA AGAACTGAGA ATTTTAGTGG GAACAAATTT GGTGCGCCTC 
AGTCAGTTGG AAGGTGTAAA TGTGGAACGT TACAAACAGA TTGTTTTGAC TGGCATATTG 
GAGCAAGTTG TAAACTGTAG GGATGCTTTG GCTCAAGAAT ATCTCATGGA GTGTATTATT 
CAGGTTTTCC CTGATGAATT TCACCTCCAG ACTTTGAATC CTTTTCTTCG GGCCTGTGCT 
GAGTTACACC AGAATGTAAA TGTGAAGAAC ATAATCATTG CTTTAATTGA TAGATTAGCT 
TTATTTGCTC ACCGTGAAGA TGGACCTGGA ATCCCAGCGG ATATTAAACT TTTTGATATA 
TTTTCACAGC AGGTGGCTAC AGTGATACAG TCTAGACAAG ACATGCCTTC AGAGGATGTT 
GTATCTTTAC AAGTCTCTCT GATTAATCTT GCCATGAAAT GTTACCCTGA TCGTGTGGAC 
TATGTTGATA AAGTTCTAGA AACAACAGTG GAGATATTCA ATAAGCTCAA CCTTGAACAT 
ATTGCTACCA GTAGTGCAGT TTCAAAGGAA CTCACCAGAC TTTTGAAAAT ACCAGTTGAC 
ACTTACAACA ATATTTTAAC AGTCTTGAAA TTAAAACATT TTCACCCACT CTTTGAGTAC 
TTTGACTACG AGTCCAGAAA GAGCATGAGT TGTTATGTGC TTAGTAATGT TCTGGATTAT 
AACACAGAAA TTGTCTCTCA AGACCAGGTG GATTCCATAA TGAATTTGGT ATCCACGTTG 
ATTCAAGATC AGCCAGATCA ACCTGTAGAA GACCCTGATC CAGAAGATTT TGCTGATGAG 
CAGAGCCTTG TGGGCCGCTT CATTCATCTG CTGCGCTCTG AGGACCCTGA CCAGCAGTAC 
TTGATTTTGA ACACAGCACG AAAACATTTT GGAGCTGGTG GAAATCAGCG GATTCGCTTC 
ACACTGCCAC CTTTGGTATT TGCAGCTTAC CAGCTGGCTT TTCGATATAA AGAGAATTCT 
AAAGTGGATG ACAAATGGGA AAAGAAATGC CAGAAGATTT TTTCATTTGC CCACCAGACT 
ATCAGTGCTT TGATCAAAGC AGAGCTGGCA GAATTGCCCT TAAGACTTTT TCTTCAAGGA 
GCACTAGCTG CTGGGGAAAT TGGTTTTGAA AATCATGAGA CAGTCGCATA TGAATTCATG 
TCCCAGGCAT TTTCTCTGTA TGAAGATGAA ATCAGCGATT CCAAAGCACA GCTAGCTGCC 
ATCACCTTGA TCATTGGCAC TTTTGAAAGG ATGAAGTGCT TCAGTGAAGA GAATCACGAA 
CCTCTGAGGA CTCAGTGTGC CCTTGCTGCA TCCAAACTTC TAAAGAAACC TGATCAGGGC 
CGAGCTGTGA GCACCTGTGC ACATCTCTTC TGGTCTGGCA GAAACACGGA CAAAAATGGG 
GAGGAGCTTC ACGGAGGCAA GAGGGTAATG GAGTGCCTAA AAAAAGCTCT AAAAATAGCA 
AATCAGTGCA TGGACCCCTC TCTACAAGTG CAGCTTTTTA TAGAAATTCT GAACAGATAT 
ATCTATTTTT ATGAAAAGGA AAATGATGCG GTAACAATTC AGGTTTTAAA CCAGCTTATC 
CAAAAGATTC GAGAAGACCT CCCGAATCTT GAATCCAGTG AAGAAACAGA GCAGATTAAC 
AAACATTTTC ATAACACACT GGAGCATTTG CGCTTGCGGC GGGAATCACC AGAATCCGAG 
GGGCCAATTT ATGAAGGTCT CATCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005256102.1
CDS	186..2375
Translation	MAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL

Target ORF information:

RefSeq Version	XM_005256045.3
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005256045.3

ORF Insert Sequence:

ATGGCCATTT CTGATGAACT GCACTACTTG GAGGTCTACC TGACAGATGA GTTTGCTAAA 
GGAAGGAAAG TGGCAGATCT CTACGAACTT GTACAGTATG CTGGAAACAT TATCCCAAGG 
CTTTACCTTT TGATCACAGT TGGAGTTGTA TATGTCAAGT CATTTCCTCA GTCCAGGAAG 
GATATTTTGA AAGATTTGGT AGAAATGTGC CGTGGTGTGC AACATCCCTT GAGGGGTCTG 
TTTCTTCGAA ATTACCTTCT TCAGTGTACC AGAAATATCT TACCTGATGA AGGAGAGCCA 
ACAGATGAAG AAACAACTGG TGACATCAGT GATTCCATGG ATTTTGTACT GCTCAACTTT 
GCAGAAATGA ACAAGCTCTG GGTGCGAATG CAGCATCAGG GACATAGCCG AGATAGAGAA 
AAAAGAGAAC GAGAAAGACA AGAACTGAGA ATTTTAGTGG GAACAAATTT GGTGCGCCTC 
AGTCAGTTGG AAGGTGTAAA TGTGGAACGT TACAAACAGA TTGTTTTGAC TGGCATATTG 
GAGCAAGTTG TAAACTGTAG GGATGCTTTG GCTCAAGAAT ATCTCATGGA GTGTATTATT 
CAGGTTTTCC CTGATGAATT TCACCTCCAG ACTTTGAATC CTTTTCTTCG GGCCTGTGCT 
GAGTTACACC AGAATGTAAA TGTGAAGAAC ATAATCATTG CTTTAATTGA TAGATTAGCT 
TTATTTGCTC ACCGTGAAGA TGGACCTGGA ATCCCAGCGG ATATTAAACT TTTTGATATA 
TTTTCACAGC AGGTGGCTAC AGTGATACAG TCTAGACAAG ACATGCCTTC AGAGGATGTT 
GTATCTTTAC AAGTCTCTCT GATTAATCTT GCCATGAAAT GTTACCCTGA TCGTGTGGAC 
TATGTTGATA AAGTTCTAGA AACAACAGTG GAGATATTCA ATAAGCTCAA CCTTGAACAT 
ATTGCTACCA GTAGTGCAGT TTCAAAGGAA CTCACCAGAC TTTTGAAAAT ACCAGTTGAC 
ACTTACAACA ATATTTTAAC AGTCTTGAAA TTAAAACATT TTCACCCACT CTTTGAGTAC 
TTTGACTACG AGTCCAGAAA GAGCATGAGT TGTTATGTGC TTAGTAATGT TCTGGATTAT 
AACACAGAAA TTGTCTCTCA AGACCAGGTG GATTCCATAA TGAATTTGGT ATCCACGTTG 
ATTCAAGATC AGCCAGATCA ACCTGTAGAA GACCCTGATC CAGAAGATTT TGCTGATGAG 
CAGAGCCTTG TGGGCCGCTT CATTCATCTG CTGCGCTCTG AGGACCCTGA CCAGCAGTAC 
TTGATTTTGA ACACAGCACG AAAACATTTT GGAGCTGGTG GAAATCAGCG GATTCGCTTC 
ACACTGCCAC CTTTGGTATT TGCAGCTTAC CAGCTGGCTT TTCGATATAA AGAGAATTCT 
AAAGTGGATG ACAAATGGGA AAAGAAATGC CAGAAGATTT TTTCATTTGC CCACCAGACT 
ATCAGTGCTT TGATCAAAGC AGAGCTGGCA GAATTGCCCT TAAGACTTTT TCTTCAAGGA 
GCACTAGCTG CTGGGGAAAT TGGTTTTGAA AATCATGAGA CAGTCGCATA TGAATTCATG 
TCCCAGGCAT TTTCTCTGTA TGAAGATGAA ATCAGCGATT CCAAAGCACA GCTAGCTGCC 
ATCACCTTGA TCATTGGCAC TTTTGAAAGG ATGAAGTGCT TCAGTGAAGA GAATCACGAA 
CCTCTGAGGA CTCAGTGTGC CCTTGCTGCA TCCAAACTTC TAAAGAAACC TGATCAGGGC 
CGAGCTGTGA GCACCTGTGC ACATCTCTTC TGGTCTGGCA GAAACACGGA CAAAAATGGG 
GAGGAGCTTC ACGGAGGCAA GAGGGTAATG GAGTGCCTAA AAAAAGCTCT AAAAATAGCA 
AATCAGTGCA TGGACCCCTC TCTACAAGTG CAGCTTTTTA TAGAAATTCT GAACAGATAT 
ATCTATTTTT ATGAAAAGGA AAATGATGCG GTAACAATTC AGGTTTTAAA CCAGCTTATC 
CAAAAGATTC GAGAAGACCT CCCGAATCTT GAATCCAGTG AAGAAACAGA GCAGATTAAC 
AAACATTTTC ATAACACACT GGAGCATTTG CGCTTGCGGC GGGAATCACC AGAATCCGAG 
GGGCCAATTT ATGAAGGTCT CATCCTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS35 ( XM_011523227.2 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521529.1 Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OHu69690
Clone ID Related Accession (Same CDS sequence)	XM_011523227.2 , XM_011523227.3
Accession Version	XM_011523227.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 6, 2016 this sequence version replaced XM_011523227.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Homo sapiens Annotation Release 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC 
ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG 
AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC 
CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT 
TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT 
CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT 
GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA 
ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA 
GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG 
TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA 
GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT 
TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA 
CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT 
GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA 
CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT 
TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT 
GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT 
ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC 
AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC 
TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA 
GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA 
GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC 
CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT 
TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG 
CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG 
GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT 
GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA 
GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG 
GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC 
TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG 
AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT 
GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG 
CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG 
TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT 
TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG 
ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT 
TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA 
ATTTATGAAG GTCTCATCCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521529.1
CDS	917..3220
Translation	MKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL

Target ORF information:

RefSeq Version	XM_011523227.2
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523227.2

ORF Insert Sequence:

ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC 
ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG 
AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC 
CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT 
TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT 
CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT 
GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA 
ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA 
GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG 
TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA 
GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT 
TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA 
CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT 
GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA 
CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT 
TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT 
GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT 
ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC 
AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC 
TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA 
GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA 
GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC 
CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT 
TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG 
CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG 
GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT 
GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA 
GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG 
GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC 
TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG 
AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT 
GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG 
CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG 
TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT 
TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG 
ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT 
TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA 
ATTTATGAAG GTCTCATCCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS35 ( XM_011523227.3 ) cDNA ORF clone, Homo sapiens(human) -> XP_011521529.1 Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X1, mRNA.

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CloneID	OHu69690
Clone ID Related Accession (Same CDS sequence)	XM_011523227.2 , XM_011523227.3
Accession Version	XM_011523227.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2304bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-05
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 35 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000016.10) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 26, 2018 this sequence version replaced XM_011523227.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Updated annotation Annotation Name :: Homo sapiens Updated Annotation Release 109.20191205 Annotation Version :: 109.20191205 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; propagated RefSeq model Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC 
ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG 
AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC 
CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT 
TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT 
CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT 
GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA 
ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA 
GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG 
TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA 
GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT 
TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA 
CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT 
GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA 
CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT 
TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT 
GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT 
ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC 
AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC 
TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA 
GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA 
GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC 
CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT 
TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG 
CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG 
GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT 
GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA 
GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG 
GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC 
TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG 
AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT 
GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG 
CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG 
TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT 
TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG 
ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT 
TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA 
ATTTATGAAG GTCTCATCCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_011521529.1
CDS	916..3219
Translation	MKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL

Target ORF information:

RefSeq Version	XM_011523227.3
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS35 retromer complex component (VPS35), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_011523227.3

ORF Insert Sequence:

ATGAAGAGAT GCCTGGACAA AAACAAGCTT ATGGATGCTC TAAAACATGC TTCTAATATG 
CTTGGTGAAC TCCGGACTTC TATGTTATCA CCAAAGAGTT ACTATGAACT TTATATGGCC 
ATTTCTGATG AACTGCACTA CTTGGAGGTC TACCTGACAG ATGAGTTTGC TAAAGGAAGG 
AAAGTGGCAG ATCTCTACGA ACTTGTACAG TATGCTGGAA ACATTATCCC AAGGCTTTAC 
CTTTTGATCA CAGTTGGAGT TGTATATGTC AAGTCATTTC CTCAGTCCAG GAAGGATATT 
TTGAAAGATT TGGTAGAAAT GTGCCGTGGT GTGCAACATC CCTTGAGGGG TCTGTTTCTT 
CGAAATTACC TTCTTCAGTG TACCAGAAAT ATCTTACCTG ATGAAGGAGA GCCAACAGAT 
GAAGAAACAA CTGGTGACAT CAGTGATTCC ATGGATTTTG TACTGCTCAA CTTTGCAGAA 
ATGAACAAGC TCTGGGTGCG AATGCAGCAT CAGGGACATA GCCGAGATAG AGAAAAAAGA 
GAACGAGAAA GACAAGAACT GAGAATTTTA GTGGGAACAA ATTTGGTGCG CCTCAGTCAG 
TTGGAAGGTG TAAATGTGGA ACGTTACAAA CAGATTGTTT TGACTGGCAT ATTGGAGCAA 
GTTGTAAACT GTAGGGATGC TTTGGCTCAA GAATATCTCA TGGAGTGTAT TATTCAGGTT 
TTCCCTGATG AATTTCACCT CCAGACTTTG AATCCTTTTC TTCGGGCCTG TGCTGAGTTA 
CACCAGAATG TAAATGTGAA GAACATAATC ATTGCTTTAA TTGATAGATT AGCTTTATTT 
GCTCACCGTG AAGATGGACC TGGAATCCCA GCGGATATTA AACTTTTTGA TATATTTTCA 
CAGCAGGTGG CTACAGTGAT ACAGTCTAGA CAAGACATGC CTTCAGAGGA TGTTGTATCT 
TTACAAGTCT CTCTGATTAA TCTTGCCATG AAATGTTACC CTGATCGTGT GGACTATGTT 
GATAAAGTTC TAGAAACAAC AGTGGAGATA TTCAATAAGC TCAACCTTGA ACATATTGCT 
ACCAGTAGTG CAGTTTCAAA GGAACTCACC AGACTTTTGA AAATACCAGT TGACACTTAC 
AACAATATTT TAACAGTCTT GAAATTAAAA CATTTTCACC CACTCTTTGA GTACTTTGAC 
TACGAGTCCA GAAAGAGCAT GAGTTGTTAT GTGCTTAGTA ATGTTCTGGA TTATAACACA 
GAAATTGTCT CTCAAGACCA GGTGGATTCC ATAATGAATT TGGTATCCAC GTTGATTCAA 
GATCAGCCAG ATCAACCTGT AGAAGACCCT GATCCAGAAG ATTTTGCTGA TGAGCAGAGC 
CTTGTGGGCC GCTTCATTCA TCTGCTGCGC TCTGAGGACC CTGACCAGCA GTACTTGATT 
TTGAACACAG CACGAAAACA TTTTGGAGCT GGTGGAAATC AGCGGATTCG CTTCACACTG 
CCACCTTTGG TATTTGCAGC TTACCAGCTG GCTTTTCGAT ATAAAGAGAA TTCTAAAGTG 
GATGACAAAT GGGAAAAGAA ATGCCAGAAG ATTTTTTCAT TTGCCCACCA GACTATCAGT 
GCTTTGATCA AAGCAGAGCT GGCAGAATTG CCCTTAAGAC TTTTTCTTCA AGGAGCACTA 
GCTGCTGGGG AAATTGGTTT TGAAAATCAT GAGACAGTCG CATATGAATT CATGTCCCAG 
GCATTTTCTC TGTATGAAGA TGAAATCAGC GATTCCAAAG CACAGCTAGC TGCCATCACC 
TTGATCATTG GCACTTTTGA AAGGATGAAG TGCTTCAGTG AAGAGAATCA CGAACCTCTG 
AGGACTCAGT GTGCCCTTGC TGCATCCAAA CTTCTAAAGA AACCTGATCA GGGCCGAGCT 
GTGAGCACCT GTGCACATCT CTTCTGGTCT GGCAGAAACA CGGACAAAAA TGGGGAGGAG 
CTTCACGGAG GCAAGAGGGT AATGGAGTGC CTAAAAAAAG CTCTAAAAAT AGCAAATCAG 
TGCATGGACC CCTCTCTACA AGTGCAGCTT TTTATAGAAA TTCTGAACAG ATATATCTAT 
TTTTATGAAA AGGAAAATGA TGCGGTAACA ATTCAGGTTT TAAACCAGCT TATCCAAAAG 
ATTCGAGAAG ACCTCCCGAA TCTTGAATCC AGTGAAGAAA CAGAGCAGAT TAACAAACAT 
TTTCATAACA CACTGGAGCA TTTGCGCTTG CGGCGGGAAT CACCAGAATC CGAGGGGCCA 
ATTTATGAAG GTCTCATCCT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

VPS35 ( NM_018206.6 ) cDNA ORF clone, Homo sapiens(human) -> NP_060676.2 Homo sapiens VPS35 retromer complex component (VPS35), mRNA.

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CloneID	OHu108594
Clone ID Related Accession (Same CDS sequence)	NM_018206.6
Accession Version	NM_018206.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2391bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-10
Organism	Homo sapiens(human)
Product	vacuolar protein sorting-associated protein 35
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC007225.2 and AC012186.8. On May 17, 2019 this sequence version replaced NM_018206.5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1803613.283710.1, SRR1803611.161623.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000299138.12/ ENSP00000299138.7 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG 
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA 
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC 
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT 
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG 
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG 
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC 
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG 
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG 
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG 
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG 
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA 
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT 
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT 
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG 
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA 
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA 
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC 
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA 
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT 
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG 
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA 
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT 
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT 
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT 
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT 
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT 
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC 
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG 
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT 
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC 
TTCAGTGAAG AGAATCACGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT 
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC 
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA 
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT 
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT 
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT 
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG 
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_060676.2
CDS	46..2436
Translation	MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL

Target ORF information:

RefSeq Version	NM_018206.6
Organism	Homo sapiens(human)
Definition	Homo sapiens VPS35 retromer complex component (VPS35), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_018206.6

ORF Insert Sequence:

ATGCCTACAA CACAGCAGTC CCCTCAGGAT GAGCAGGAAA AGCTCTTGGA TGAAGCCATA 
CAGGCTGTGA AGGTCCAGTC ATTCCAAATG AAGAGATGCC TGGACAAAAA CAAGCTTATG 
GATGCTCTAA AACATGCTTC TAATATGCTT GGTGAACTCC GGACTTCTAT GTTATCACCA 
AAGAGTTACT ATGAACTTTA TATGGCCATT TCTGATGAAC TGCACTACTT GGAGGTCTAC 
CTGACAGATG AGTTTGCTAA AGGAAGGAAA GTGGCAGATC TCTACGAACT TGTACAGTAT 
GCTGGAAACA TTATCCCAAG GCTTTACCTT TTGATCACAG TTGGAGTTGT ATATGTCAAG 
TCATTTCCTC AGTCCAGGAA GGATATTTTG AAAGATTTGG TAGAAATGTG CCGTGGTGTG 
CAACATCCCT TGAGGGGTCT GTTTCTTCGA AATTACCTTC TTCAGTGTAC CAGAAATATC 
TTACCTGATG AAGGAGAGCC AACAGATGAA GAAACAACTG GTGACATCAG TGATTCCATG 
GATTTTGTAC TGCTCAACTT TGCAGAAATG AACAAGCTCT GGGTGCGAAT GCAGCATCAG 
GGACATAGCC GAGATAGAGA AAAAAGAGAA CGAGAAAGAC AAGAACTGAG AATTTTAGTG 
GGAACAAATT TGGTGCGCCT CAGTCAGTTG GAAGGTGTAA ATGTGGAACG TTACAAACAG 
ATTGTTTTGA CTGGCATATT GGAGCAAGTT GTAAACTGTA GGGATGCTTT GGCTCAAGAA 
TATCTCATGG AGTGTATTAT TCAGGTTTTC CCTGATGAAT TTCACCTCCA GACTTTGAAT 
CCTTTTCTTC GGGCCTGTGC TGAGTTACAC CAGAATGTAA ATGTGAAGAA CATAATCATT 
GCTTTAATTG ATAGATTAGC TTTATTTGCT CACCGTGAAG ATGGACCTGG AATCCCAGCG 
GATATTAAAC TTTTTGATAT ATTTTCACAG CAGGTGGCTA CAGTGATACA GTCTAGACAA 
GACATGCCTT CAGAGGATGT TGTATCTTTA CAAGTCTCTC TGATTAATCT TGCCATGAAA 
TGTTACCCTG ATCGTGTGGA CTATGTTGAT AAAGTTCTAG AAACAACAGT GGAGATATTC 
AATAAGCTCA ACCTTGAACA TATTGCTACC AGTAGTGCAG TTTCAAAGGA ACTCACCAGA 
CTTTTGAAAA TACCAGTTGA CACTTACAAC AATATTTTAA CAGTCTTGAA ATTAAAACAT 
TTTCACCCAC TCTTTGAGTA CTTTGACTAC GAGTCCAGAA AGAGCATGAG TTGTTATGTG 
CTTAGTAATG TTCTGGATTA TAACACAGAA ATTGTCTCTC AAGACCAGGT GGATTCCATA 
ATGAATTTGG TATCCACGTT GATTCAAGAT CAGCCAGATC AACCTGTAGA AGACCCTGAT 
CCAGAAGATT TTGCTGATGA GCAGAGCCTT GTGGGCCGCT TCATTCATCT GCTGCGCTCT 
GAGGACCCTG ACCAGCAGTA CTTGATTTTG AACACAGCAC GAAAACATTT TGGAGCTGGT 
GGAAATCAGC GGATTCGCTT CACACTGCCA CCTTTGGTAT TTGCAGCTTA CCAGCTGGCT 
TTTCGATATA AAGAGAATTC TAAAGTGGAT GACAAATGGG AAAAGAAATG CCAGAAGATT 
TTTTCATTTG CCCACCAGAC TATCAGTGCT TTGATCAAAG CAGAGCTGGC AGAATTGCCC 
TTAAGACTTT TTCTTCAAGG AGCACTAGCT GCTGGGGAAA TTGGTTTTGA AAATCATGAG 
ACAGTCGCAT ATGAATTCAT GTCCCAGGCA TTTTCTCTGT ATGAAGATGA AATCAGCGAT 
TCCAAAGCAC AGCTAGCTGC CATCACCTTG ATCATTGGCA CTTTTGAAAG GATGAAGTGC 
TTCAGTGAAG AGAATCACGA ACCTCTGAGG ACTCAGTGTG CCCTTGCTGC ATCCAAACTT 
CTAAAGAAAC CTGATCAGGG CCGAGCTGTG AGCACCTGTG CACATCTCTT CTGGTCTGGC 
AGAAACACGG ACAAAAATGG GGAGGAGCTT CACGGAGGCA AGAGGGTAAT GGAGTGCCTA 
AAAAAAGCTC TAAAAATAGC AAATCAGTGC ATGGACCCCT CTCTACAAGT GCAGCTTTTT 
ATAGAAATTC TGAACAGATA TATCTATTTT TATGAAAAGG AAAATGATGC GGTAACAATT 
CAGGTTTTAA ACCAGCTTAT CCAAAAGATT CGAGAAGACC TCCCGAATCT TGAATCCAGT 
GAAGAAACAG AGCAGATTAA CAAACATTTT CATAACACAC TGGAGCATTT GCGCTTGCGG 
CGGGAATCAC CAGAATCCGA GGGGCCAATT TATGAAGGTC TCATCCTTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Parkinson's disease-associated pathogenic VPS35 mutation causes complex I deficits.
Biochimica et biophysica acta1863(11)2791-2795(2017 Nov)
Zhou L,Wang W,Hoppel C,Liu J,Zhu X

Retriever is a multiprotein complex for retromer-independent endosomal cargo recycling.
Nature cell biology19(10)1214-1225(2017 Oct)
McNally KE,Faulkner R,Steinberg F,Gallon M,Ghai R,Pim D,Langton P,Pearson N,Danson CM,N?gele H,Morris LL,Singla A,Overlee BL,Heesom KJ,Sessions R,Banks L,Collins BM,Berger I,Billadeau DD,Burstein E,Cullen PJ

Architecture of the human interactome defines protein communities and disease networks.
Nature545(7655)505-509(2017 05)
Huttlin EL,Bruckner RJ,Paulo JA,Cannon JR,Ting L,Baltier K,Colby G,Gebreab F,Gygi MP,Parzen H,Szpyt J,Tam S,Zarraga G,Pontano-Vaites L,Swarup S,White AE,Schweppe DK,Rad R,Erickson BK,Obar RA,Guruharsha KG,Li K,Artavanis-Tsakonas S,Gygi SP,Harper JW

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VPS35 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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